• CD14 antigen is strongly expressed on monocytes, macrophages, and weakly on neutrophils. (beckman.com)
  • In contrast, HLA-G tetramers did bind to peripheral blood monocytes, staining a CD16(+)CD14(mid) subset with greater intensity. (ox.ac.uk)
  • 99% of all CD14 positive monocytes express CD163 and that monocyte derived dendritic cells express low levels of CD163. (nih.gov)
  • The MoP9 antibody reacts with CD14, a 53 - 55 kDa glycosylphosphatidylinositol (GPI)-anchored transmembrane glycoprotein expressed at high levels on the surface of peripheral blood monocytes and macrophages, and at lower levels on granulocytes. (stemcell.com)
  • An approximate 10-fold difference in expression levels between monocytes/macrophages and granulocytes makes CD14 a useful marker for distinguishing these cell populations. (stemcell.com)
  • We show here that these novel intrathymic DCs are truly myeloid, as they arise from CD34 + early thymic progenitors through CD34 lo intermediates which have lost the capacity to generate T cells, but display myelomonocytic differentiation potential. (ashpublications.org)
  • Two soluble forms of CD14 have also been described (~48 and ~55 kDa). (stemcell.com)
  • Moreover, although interleukin 7 (IL-7) supports the generation of such myeloid intermediates, we show that their developmental branching from the main intrathymic T-cell pathway is linked to the up-regulation of the myelomonocytic granulocyte macrophage-colony-stimulating factor (GM-CSF) receptor, to the down-regulation of the IL-7 receptor and to the lack of pre-T-cell receptor α (pTα) gene transcriptional activation. (ashpublications.org)
  • CD14 functions as a high-affinity receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein and modulates LPS-dependent signal transduction during the immune response to gram-negative pathogens by acting as a co-receptor for TLR 4 and MD-2. (stemcell.com)
  • CD135 is a 130-160 kD type III tyrosine kinase receptor expressed on CD34 + hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. (biolegend.com)
  • Within myeloid neoplasms, chronic myelomonocytic leukemia (CMML) represents an attractive disease model since it is characterized by both myelodysplastic and myeloproliferative features, while retaining a relatively simple clonal composition 3 . (nature.com)
  • The CD14 antigen is a glycosyl-phosphatidylinositol-linked single-chain surface membrane glycoprotein with a molecular weight of 53-55 kDa. (beckman.com)
  • Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells. (ox.ac.uk)
  • It is found on cells of myelomonocytic lineage. (beckman.com)
  • Expressed on CD34 + hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. (biolegend.com)
  • Functional recombinant FRβ protein was produced in insect cells and used as antigen to isolate a mAb, m909, from a human naïve Fab phage display library. (biomedcentral.com)
  • The nonclassical MHC class I molecule human histocompatibility leukocyte antigen (HLA)-G is selectively expressed on fetal trophoblast tissue at the maternal-fetal interface in pregnancy. (ox.ac.uk)
  • Taken together, these data challenge the current view that the thymus is colonized by a lymphoid-restricted progenitor and provide evidence that a more immature precursor population with lymphoid and myelomonocytic potential is actually seeding the human postnatal thymus. (ashpublications.org)
  • Figure A. RPE conjugated Mouse anti Human CD14 ( MCA1568PE ) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control ( MCA928A647 ). (bio-rad-antibodies.com)
  • Figure B. RPE conjugated Mouse anti Human CD14 ( MCA1568PE ) and Alexa Fluor 647 conjugated Mouse anti Human CD11b ( MCA551A647 ). (bio-rad-antibodies.com)
  • Perform human leukocyte antigen (HLA) or DNA typing in patients who are potential candidates for allogeneic transplantation . (medscape.com)
  • These results suggest that the primary role of HLA-G may be the modulation of myelomonocytic cell behavior in pregnancy. (ox.ac.uk)
  • We performed a multi-omics interrogation to define gene expression and chromatin remodeling associated with ASXL1 MT in chronic myelomonocytic leukemia (CMML). (nature.com)
  • Meanwhile, the widespread decreased antigen presentation ability and immune disorders caused by deletion of MHC class II gene expression should not be ignored. (bvsalud.org)
  • Immunohistochemistry performed on this myeloid sarcoma involving the salivary gland showed the large neoplastic cells to be diffusely positive for CD34 (upper left), CD117 (upper right), and CD43 (lower left), as well as variably positive for myeloperoxidase (MPO) (middle left), lysozyme (middle right), and CD68 (lower right), indicative of both myeloid and monocytic (ie, myelomonocytic) differentiation. (medscape.com)
  • 2-4 Anti-CD14 can detect these cells, including monocyte-derived cells which are frequently increased in diffuse large B-cell lymphoma (DLBCL), 3 as well as in neoplastic cells in acute myeloid leukemia with monocytic differentiation and chronic myelomonocytic leukemia. (roche.com)
  • To determine whether immunophenotypic features of monocytes are useful in differentiating chronic myelomonocytic leukemia (CMML) from reactive monocytosis, multiparameter flow cytometry was used to immunophenotype 20 bone marrow samples from patients with CMML, 10 normal marrow samples, and 20 marrow samples with reactive monocytosis. (medscape.com)
  • Monocytes in CMML exhibited aberrant antigen expression in all 20 cases. (medscape.com)
  • Abnormal antigen expression also was observed in monocytes in 11 of 20 reactive marrow samples. (medscape.com)
  • Subpopulations of monocytes with moderate levels of CD14 were present in all 3 groups. (medscape.com)
  • The proportion of CD14(moderate) monocytes was highest in CMML and was 20% or more in 13 of 20 CMML cases vs 3 of 20 reactive marrow samples ( P = .003) and 2 of 10 normal marrow samples ( P = .007). (medscape.com)
  • A combination of monocytosis with 2 or more immunophenotypic aberrancies with 20% or more of marrow monocytes showing moderate CD14 expression was 67% sensitive and 100% specific for CMML. (medscape.com)
  • Imbalanced Inflammatory Responses in Preterm and Term Cord Blood Monocytes and Expansion of the CD14+CD16+ Subset upon Toll-like Receptor Stimulation. (ucdenver.edu)
  • In addition, monocytes serve as antigen-presenting cells and migrate into tissues. (pocketdentistry.com)
  • 3. Immunohistochemical analysis of monocytic leukemias: usefulness of CD14 and Kruppel-like factor 4, a novel monocyte marker. (nih.gov)
  • Here, we report that human NOX2 + myelomonocytic/monocytic AML cell lines showed increased expression of maturation markers along with reduced leukemic cell proliferation after exposure to HDC in vitro . (frontiersin.org)
  • In addition, post hoc analyses of phase III trial results suggested that HDC/IL-2 may be preferentially or specifically efficacious in patients where the leukemic clone is dominated by monocytic cells, i.e., myelomonocytic (FAB class M4) or monocytic (M5) AML ( 7 ). (frontiersin.org)
  • CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. (roche.com)
  • Chronic myelomonocytic leukemia (CMML) is a clonal stem cell disorder defined as a myeloproliferative/myelodysplastic disease by the World Health Organization based on the variability of the presenting WBC count, marrow cellularity, degree of dysplasia, and splenomegaly. (medscape.com)
  • 6] Staining for CD45 (leukocyte common antigen), terminal deoxynucleotidyl transferase (TdT), CD43, CD56, and CD99 is variable and not lineage defining. (medscape.com)
  • Outlined tumor antigen-specific T cells potentiate personalised TCR-T cell remedy and prediction of immunotherapy response. (superpcparts.com)
  • Figuring out and focusing on human tumor antigens for T cell-based immunotherapy of strong tumors. (superpcparts.com)
  • Antigens, CD15" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
  • In addition, CD15 antigen is a stage-specific embryonic antigen. (childrensmercy.org)
  • This graph shows the total number of publications written about "Antigens, CD15" by people in this website by year, and whether "Antigens, CD15" was a major or minor topic of these publications. (childrensmercy.org)
  • Below are the most recent publications written about "Antigens, CD15" by people in Profiles. (childrensmercy.org)
  • Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "CD14 (EPR3653) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the detection of the CD14 protein in formalin-fixed, paraffin-embedded tissue stained on VENTANA BenchMark IHC/ISH instruments. (roche.com)
  • Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "CD14 is a 55kDa glycosyl-phosphatidylinositol-linked membrane protein, involved in endotoxin binding and recognition of apoptotic cells. (roche.com)
  • The value of immunohistochemistry for CD14, CD123, CD33, myeloperoxidase and CD68R in the diagnosis of acute and chronic myelomonocytic leukaemias. (roche.com)
  • Three patients had acquired copy-neutral LOH (CN-LOH) on 6p arms, which may disrupt antigen presentation and act as a mechanism for immune system evasion. (oncotarget.com)
  • It has also been proposed that DCs are intimately involved in the prevention of inappropriate immune responses to "self"-antigens. (ashpublications.org)
  • Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Principle", "Value": "CD14 (EPR3653) Rabbit Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. (roche.com)
  • In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. (roche.com)
  • We performed qRT-PCR and FACS on human VLC to assess the expression of the CD52 antigen, the target of the immunotherapeutic Alemtuzumab. (biomedcentral.com)
  • However, aberrant expression of 2 or more antigens was significantly less frequent in reactive monocytosis than in CMML ( P = .002). (medscape.com)