Myeloid precursors granulocytic differentiation of murine hematopoietic cells. Medical search. Frequent questions

The innate immune system is mainly composed of myeloid lineage cells, such as macrophages, neutrophils, and mast cells [ 2 ]. (hindawi.com)
Although tumor cells were first thought to drive the cellular events underpinning tumor angiogenesis and growth, considerable evidence has now emerged for the central role of tumor infiltrating myeloid cells such as monocytes, macrophages, and neutrophils in this phenomenon [ 8 - 12 ]. (hindawi.com)
Mononuclear myeloid cells include terminally differentiated macrophages and dendritic cells (DC), as well as monocytes, which under inflammatory conditions differentiate in tissues to macrophages and DCs. (aacrjournals.org)
CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. (thermofisher.com)
Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. (thermofisher.com)
Finally, MO-MDSCs were shown to be potential precursors of highly antiproliferative NO-producing mature macrophages. (ashpublications.org)

It may also mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. (wikipedia.org)
Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones (B lymphocyte precursors) and CD34+ megakaryocytes. (wikipedia.org)
Myeloid cells are a heterogeneous population of bone marrow-derived cells that play a critical role during growth and metastasis of malignant tumors. (hindawi.com)
These cells were therefore described as bone marrow derived endothelial progenitor cells (EPCs). (hindawi.com)
Although these disorders share certain clinical features-including bone marrow hypercellularity, frequent splenomegaly, and risks of thrombosis, hemorrhage, and transformation to acute myeloid leukemia (AML)-they also show important phenotypic differences. (oncohemakey.com)
In PV, bone marrow panmyelosis is associated with increased numbers of red blood cells and, in some patients, neutrophilia and/or thrombocytosis. (oncohemakey.com)
Evidence suggests that P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) are one of the cell-cell adhesion ligand-receptor pairs for HSCs to keep contacting bone marrow (BM) stromal cells before being mobilized into circulation. (bvsalud.org)
Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
Effective hematopoiesis is facilitated by interactions of hematopoietic growth factors, various receptors, and the bone marrow microenvironment. (medilib.ir)
in turn, each of these categories is classified as either acute or chronic, depending on the proportion of morphologically and immunophenotypically immature precursors (blasts) in the bone marrow or peripheral blood. (medilib.ir)
bone marrow or peripheral blood blasts of 20 percent or more) and the chronic myeloid disorders (bone marrow or peripheral blood blasts less than 20 percent). (medilib.ir)
The paradox of proliferative bone marrow together with peripheral blood cytopenias in MDS may be explained by increased intramedullary myeloid precursor cell apoptosis [ 6 ]. (medilib.ir)

The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. (wikipedia.org)
The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
Vasculogenesis is the coalescence of new blood vessels from individual endothelial cells or progenitor cells. (hindawi.com)
Moreover, in human studies, JAK2V617F has been identified in the stem cell-enriched CD34 + CD38 − CD90 + Lin − compartment, common myeloid progenitors, granulocyte-monocyte precursors, and megakaryocyte-erythroid precursors as well as natural killer cells, B cells, and T cells in some patients, confirming its origin in an early hematopoietic progenitor. (oncohemakey.com)
Clonal studies in the chronic myeloid disorders - Genetic and enzyme studies based upon X-chromosome inactivation patterns have revealed a multipotent progenitor cell origin for the neoplastic clone in both MDS [ 8 ] and MPN [ 9 ]. (medilib.ir)
The clonal process may develop at different progenitor cell levels in individual patients, and the clonal progenitor cell may or may not involve T and B lymphocytes [ 10 ]. (medilib.ir)

CLASSIFICATION OF HEMATOLOGIC MALIGNANCIES - The hematopoietic pluripotent stem cell is capable of both self-renewal and stepwise differentiation into either the lymphoid or myeloid lineage (which appears to be determined stochastically) [ 1 ]. (medilib.ir)

These cells are closely related to neutrophils and monocytes. (aacrjournals.org)
Granulocytic myeloid cells include populations of terminally differentiated polymorphonuclear neutrophils, eosinophils, basophils, and mast cells. (aacrjournals.org)
Myeloid cells generated under these conditions, although similar to neutrophils and monocytes in morphology and phenotype, have different genomic and biochemical profiles and functional activity. (aacrjournals.org)
The field started changing in the late 1990s when the Gr1 + CD11b + cellular phenotype was suggested as defining the immune-suppressive myeloid cells in spleens of mice and when these cells were shown to be phenotypically similar but functionally distinct from monocytes and neutrophils ( 2, 3 ). (aacrjournals.org)
These fractions consisted of either mononuclear cells (MO-MDSCs), resembling inflammatory monocytes, or low-density polymorphonuclear cells (PMN-MDSCs), akin to immature neutrophils. (ashpublications.org)

It is now known that new blood vessels originate from preexisting vessels by activation, proliferation and migration of endothelial cells through a process named "angiogenesis" [ 4 ]. (hindawi.com)
Specific growth factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), stimulate the proliferation and migration of naturally quiescent endothelial cells, resulting in the formation of new vessel structures during embryonic development and tumor growth [ 7 ]. (hindawi.com)
CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage. (thermofisher.com)
They confer resistance against many different viruses, inhibit proliferation of normal and malignant cells, impede multiplication of intracellular parasites, enhance macrophage and granulocyte phagocytosis, augment natural killer cell activity, and show several other immunomodulatory functions. (lookformedical.com)

Depletion of Runx1 with antisense morpholino oligonucleotides abrogated the development of both blood and vessels, as demonstrated by loss of circulation, incomplete development of vasculature and the accumulation of immature hematopoietic precursors. (biologists.com)

MDS disorders have been referred to as "preleukemias" because of their tendency to transform into acute myeloid leukemia (AML). (medscape.com)

Thus, during normal hematopoiesis, there exists a cellular hierarchy headed by a stable population of pluripotent stem cells that generate lineage-specific progenitors, which differentiate into the various types of mature blood cells [ 2 ]. (medilib.ir)

Tumors exhibit significant myeloid cell infiltrates, which are actively recruited to the tumor microenvironment. (hindawi.com)
In 1971, Dr. Judah Folkman observed that neovascularization occurs around tumors and proposed that new blood vessel growth is necessary to supply nutrients and oxygen to tumor cells during exponential tumor growth [ 6 ]. (hindawi.com)
The observations of the accumulation in spleens and tumors of large numbers of these cells with potent immune-suppressive activity were readily reproducible in most murine tumor models. (aacrjournals.org)
The induction of CD11b + Gr-1 + myeloid-derived suppressor cells (MDSCs) is an important immune-evading mechanism used by tumors. (ashpublications.org)
However, distinct tumors differentially regulated this inherent MO-MDSC differentiation program, indicating that this phenomenon was tumor driven. (ashpublications.org)

13 ] isolated mononuclear cells from human peripheral blood that were enriched for expression of the hematopoietic stem cell marker CD34 [ 13 ]. (hindawi.com)

Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. (wikipedia.org)

Interestingly, both MO-MDSCs and PMN-MDSCs suppressed antigen-specific T-cell responses, albeit using distinct effector molecules and signaling pathways. (ashpublications.org)

All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)

CD34+ is often used clinically to quantify the number of haemopoietic stem cells for use in haemopoietic stem cell transplantation. (wikipedia.org)
Mutations in TET2, other epigenetic regulators, and other regulators of cytokine signaling are not specific to the classic myeloproliferative neoplasms (MPNs) but may influence prognosis and play roles in hematopoietic stem cell (HSC) dysregulation and progression to accelerated or blast-phase disease. (oncohemakey.com)
Autologous Stem Cell Transplant (ASCT) is increasingly used to treat hematological malignancies. (bvsalud.org)
MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)

The neutrophil maturation and differentiation pathway termed myelopoiesis takes approximately 12 days. (medscape.com)

Upon culture in endothelial growth media, these cells expressed endothelial lineage markers, such as CD31, Tie2, and VEGF receptor 2 (VEGFR2), and incorporated into blood vessels in ischemic tissues. (hindawi.com)
Male mice with P-selectin deficiency (Selp-/-) were further employed to investigate whether P-selectin is essential for G-CSF-induced HSC mobilization and determine which cell lineage is sP-sel derived from. (bvsalud.org)

Natural-killer (NK) cells are specialized lymphocytes that have cytotoxic properties in addition to their ability to produce cytokines that assist in the orchestration of adaptive immunity. (medscape.com)
The adaptive immune system consists of 2 types of lymphocytes: T cells (70-75% of the adaptive immune force) and B cells (10-20% of the adaptive immune force). (medscape.com)
In addition to antiviral activity, it activates NATURAL KILLER CELLS and B-LYMPHOCYTES , and down-regulates VASCULAR ENDOTHELIAL GROWTH FACTOR expression through PI-3 KINASE and MAPK KINASES signaling pathways. (lookformedical.com)
TIME possesses distinct populations of myeloid cells and lymphocytes to influence the immune escape of cancer, the response to immunotherapy, and the survival of patients. (biomedcentral.com)
TIME primarily consists of myeloid cells, lymphocytes and some other innate immune cells. (biomedcentral.com)

This suggested that EPCs are able to differentiate into endothelial cells and that such cells are incorporated into sites of active angiogenesis including ischemia, tumor angiogenesis, and metastasis in adult organisms [ 16 , 17 ]. (hindawi.com)

Injection of CD34+ hematopoietic stem cells has been clinically applied to treat various diseases including spinal cord injury, liver cirrhosis and peripheral vascular disease. (wikipedia.org)
A key requisite for ASCT is mobilization of hematopoietic stem cells into peripheral blood, where they are collected by apheresis and stored for later transplantation. (bvsalud.org)
In mice, GPC-100 mobilized more white blood cells into peripheral blood compared to plerixafor. (bvsalud.org)
Myeloproliferative neoplasms (MPN), unlike MDS, usually exhibit terminal myeloid cell expansion in the peripheral blood [ 7 ]. (medilib.ir)
One of the type I interferons produced by peripheral blood leukocytes or lymphoblastoid cells. (lookformedical.com)

Importantly, during tumor progression, myeloid cells are implicated in promoting tumor angiogenesis, causing resistance against antiangiogenic therapies in cancer, and suppressing the immune response during cancer [ 3 - 5 ]. (hindawi.com)
These cells have emerged as an important contributor to tumor progression. (aacrjournals.org)
Reports on the accumulation of immune-suppressive myeloid cells associated with tumor progression were published sporadically beginning in the early 1970s ( 1 ). (aacrjournals.org)

Cancer is not a chaotic malignant cell mass, but a delicate "hostile" organ, where many other cells are recruited and domesticated to become "accomplices", thereby protecting themselves from recognition and attack by the immune system [ 1 ]. (biomedcentral.com)

Zebrafish runx1 is expressed in hematopoietic and neuronal cells during early embryogenesis. (biologists.com)

This process is epitomized by myeloid-derived suppressor cells (MDSC). (aacrjournals.org)

The chronic myeloproliferative neoplasms (MPNs) are clonal disorders characterized by overproduction of mature myeloid cells. (oncohemakey.com)
INTRODUCTION - An overview of the four classic myeloproliferative neoplasms (MPN): polycythemia vera, essential thrombocythemia, primary myelofibrosis, and chronic myeloid leukemia will be presented here. (medilib.ir)

i.e., they are multipotent hematopoietic stem cells. (wikipedia.org)

Among the components of TME, distinct populations of innate and adaptive immune cells consist of tumor immune microenvironment (TIME). (biomedcentral.com)

During the 1980s and early 1990s, work from the laboratories of Diana Lopez, Jim Talmadge, M. Rita Young, and Hans Schreiber demonstrated that various types of myeloid cells could inhibit immune function in cancer. (aacrjournals.org)

Finally, we will comment on the mechanisms regulating myeloid cell recruitment to the tumor microenvironment and on the potential of myeloid cells as new targets for cancer therapy. (hindawi.com)

Innate immunity resides in the skin, mucous membranes, polymorphonuclear (PMN) cells, complement system, and a select group of cells that possess cytotoxic capabilities. (medscape.com)

Finally, wild-type mice were injected with either G-CSF or recombinant sP-sel to investigate whether sP-sel alone is sufficient for inducing HSC mobilization and whether it accomplishes this by binding to HSCs and disrupting their interaction with stromal cells in the BM. (bvsalud.org)
G-CSF induces the release of sP-sel from platelets, which is sufficient to mobilize BM HSCs into the circulation of mice by disrupting the PSGL-1 and P-selectin interaction between HSCs and stromal cells. (bvsalud.org)

The activating effects of the mutation are thought to reflect disruption of the normal autoinhibitory function of the JH2 domain, within which JAK2V617F is found, and have been shown to affect downstream pathways, including STATs (especially STAT5), phosphatidylinositol 3-kinase, and MAP kinase pathways in cell lines and animal models. (oncohemakey.com)
In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)

The MPNs comprise a set of clonal HSC disorders, characterized by the overproduction of 1 or more mature myeloid cell types. (oncohemakey.com)

Furthermore, we describe a subset of myeloid cells with immunosuppressive activity (known as myeloid-derived suppressor cells). (hindawi.com)
Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)

Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)
Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
and the innate immune cells contain natural killer (NK) cells and dendritic cells (DCs) (Fig. 1 ). (biomedcentral.com)

Th17 cell may have a role in severe asthma and chronic obstructive pulmonary disease (COPD). (cdc.gov)
See "Clinical manifestations and diagnosis of chronic myeloid leukemia" . (medilib.ir)
The chronic myeloid disorders - The chronic myeloid disorders encompass several clinicopathologic entities. (medilib.ir)
MPNs include polycythemia vera, essential thrombocythemia, chronic myeloid leukemia (CML), primary myelofibrosis, chronic neutrophilic leukemia, and other less well defined entities such as chronic eosinophilic leukemia, not otherwise categorized. (medilib.ir)

The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
The reason for the myeloid bias in these diseases is unclear, given the role of JAK2 downstream of numerous cytokine receptors, but could reflect qualitative differences in the consequences of JAK2V617F in the context of different receptors. (oncohemakey.com)

TME consists of cell component, the extracellular matrix (ECM) and abundant soluble signaling molecules. (biomedcentral.com)

CD34 derives its name from the cluster of differentiation protocol that identifies cell surface antigens. (wikipedia.org)

CD34 was first described on hematopoietic stem cells independently by Civin et al. (wikipedia.org)
CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. (wikipedia.org)
Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration. (wikipedia.org)
CD34 is expressed in roughly 20% of murine haematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
Subsequent studies described a VEGFR2 and AC133 expressing subpopulation of these CD34 positive circulating cells that could form endothelial colonies in vitro [ 14 , 15 ]. (hindawi.com)

It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES. (lookformedical.com)

Transient expression of runx1 in cloche embryos resulted in partial rescue of the hematopoietic defect. (biologists.com)

Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. (lookformedical.com)

as a cell surface glycoprotein and functions as a cell-cell adhesion factor. (wikipedia.org)

The main functional characteristic of these cells is their potent ability to suppress various types of immune responses. (aacrjournals.org)
Substantial cells are divided into immune cells and non-immune cells. (biomedcentral.com)
The infiltrating immune cells in the TME constitute the main body of TIME. (biomedcentral.com)

Cell-to-cell interactions set off a cascade of events that may result in T- or B-cell activation and, ultimately, host defense. (medscape.com)

We used the Video Performance Evaluation Resource (ViPER) software (Doermann and Mihalcik, 2000) to manually draw bounding boxes around cells in each video frame and index the sequences of bounding boxes corresponding JQ1 in vitro to each individual cell to designate tracks. (peptidesolubility.com)
Cells propagated in vitro in special media conducive to their growth. (lookformedical.com)

Mammalian cells require oxygen and nutrients for their survival and are therefore located within 100 to 200 m of blood vessels, the diffusion limit of oxygen. (hindawi.com)

The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)
Th9 cells were first identified as a Th2 subpopulation have many important roles. (cdc.gov)

However, in cancer, myeloid cells are largely converted to serve a new master-tumor cells. (aacrjournals.org)
In addition to tumor cells, there are also important stromal components in tumor niche. (biomedcentral.com)

runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl , and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche . (biologists.com)
PHOSPHORYLATION of IRF-3 causes the protein to be translocated from the CYTOPLASM to CELL NUCLEUS where it binds DNA , and activates transcription. (lookformedical.com)

BACKGROUND: Granulocyte colony-stimulating factor (G-CSF)-mediated mobilization of hematopoietic stem cells (HSCs) is a well-established method to prepare HSCs for transplantation nowadays. (bvsalud.org)

Myeloid cells promote tumor growth by stimulating tumor angiogenesis, suppressing tumor immunity, and promoting metastasis to distinct sites. (hindawi.com)

This study hypothesized that blockage of PSGL-1 and P-selectin may disrupt HSC-stromal cell interaction and facilitate HSC mobilization. (bvsalud.org)

This is generally a useful marker for cell dosing although there is some evidence that the CD34+ quantification may not be reliable in some circumstances. (wikipedia.org)

In the present study, we show that GPC-100 has high affinity towards the chemokine receptor CXCR4, and it potently inhibits ß-arrestin recruitment, calcium flux and cell migration mediated by its ligand CXCL12. (bvsalud.org)

Over the past two decades, a series of significant advances have been achieved in exploring the biogenesis, abundance, and function of lncRNAs across different species and cell types. (biomedcentral.com)

CD34+ cells may be isolated from blood samples using immunomagnetic techniques and used for CD34+ transplants, which have lower rates of graft-versus-host disease. (wikipedia.org)
It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. (lookformedical.com)

Anatomy57

Organisms19

Diseases13

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes56

Disciplines and Occupations1

Information Science3

Macrophages6

Bone marrow14

Progenitor6

Pluripotent stem1

Neutrophils5

Proliferation4

Immature1

Acute1

Progenitors1

Tumors5

Mononuclear cells1

Dendritic cell2

MDSCs1

Embryonic development1

Stem cell4

Myelopoiesis1

Lineage2

Lymphocytes5

Endothelial cells1

Peripheral5

Progression3

Malignant1

Neuronal1

MDSC1

Myeloproliferative2

Multipotent1

Populations1

Inhibit1

Microenvironment1

Polymorphonuclear1

Mice2

Pathways2

Clonal1

Subset2

Innate5

Chronic4

Receptors3

Extracellular matrix1

Cluster1

CD345

Antigens1

Defect1

Inflammatory1

Adhesion1

Immune3

Interactions1

Vitro2

Survival1

Roles2

Tumor cells2

Transcription2

Granulocyte1

Distinct1

Facilitate1

Marker1

Recruitment1

LncRNAs1

Transplants2