Myeloid differentiation leukemic cells. Medical search. Frequent questions

In PDX mice transplanted with human IDH2-mutant acute myeloid leukemia cells, enasidenib treatment led to normalization of (R)-2-hydroxyglutarate serum levels, differentiation of leukemic blasts and increased survival. (nih.gov)
Novel treatments for acute lymphoblastic leukemia (ALL) are utilizing the patient's T cells targeting surface molecules on the leukemic blasts. (nih.gov)
CD33 is primarily a myeloid differentiation antigen with endocytic properties broadly expressed on AML blasts and, possibly, some leukemic stem cells. (adcreview.com)
AML includes transplantable leukemia-initiating cells along with a tumor almost all myeloid cells not capable of terminal differentiation (leukemic blasts) accumulating in peripheral bloodstream and bone tissue marrow8. (exatecan-mesylate.com)
In patients samples, positive CD123 expression (CD123+) was defined as expression in 20% of leukemic blasts using MFI by comparison to background fluorescence and fluorescence on non-leukemic gated events, respectively. (nsdfu.org)
AML is characterized by inefficient blood production with progressive anemia (low numbers of red blood cells) and loss of normal mature white blood cells and platelets in the circulation that are being replaced by pernicious leukemic blasts. (ufl.edu)
In this disease, the frequency of the leukemic stem cell (LSC) was approximately 1 per million AML blasts, establishing that not every AML cell had T-IC capacity. (nih.gov)
1. The NPM-MLF1 fusion protein is expressed in blasts from patients with myelodysplasia/acute myeloid leukemia (MDS/AML) containing the t(3;5) chromosomal rearrangement. (ustc.edu.cn)
Malignant C6923 Acute Bilineal Leukemia "An acute leukemia of ambiguous lineage in which there is a dual population of blasts with each population expressing markers of a distinct lineage (myeloid and lymphoid or B-and T-lymphocyte). (nih.gov)
WHO, 2001)" Malignant C4673 Acute Biphenotypic Leukemia "An acute leukemia of ambiguous lineage characterized by blasts which coexpress myeloid and T or B lineage antigens or concurrent B and T lineage antigens. (nih.gov)
WHO, 2001)" Malignant C7464 Acute Leukemia of Ambiguous Lineage Acute Leukemia of Indeterminate Lineage "An acute leukemia in which the blasts lack sufficient evidence to classify as myeloid or lymphoid or they have morphologic and/or immunophenotypic characteristics of both myeloid and lymphoid cells. (nih.gov)
FAB M7 "An acute myeloid leukemia in which at least 50% of the blasts are of megakaryocytic lineage. (nih.gov)

Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. (fishersci.com)
Fes tyrosine kinase expression in the tumor niche correlates with enhanced tumor growth, angiogenesis, circulating tumor cells, metastasis, and infiltrating macrophages. (univ-paris5.fr)
Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. (bvsalud.org)
Additionally, ROS have been implicated in myeloid cell differentiation as high levels of intracellular ROS hinder proper differentiation of myeloid cells into dendritic cells (DC) and macrophages ( 12 ). (frontiersin.org)
GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. (lecturio.com)
Introduction Myeloid progenitors derived from multipotential hematopoietic stem cells can be differentiated into myeloid cells including neutrophils monocytes and macrophages which act as important mediators of innate immunity and play a central role in host defense against infections and to tissue damage.1-3 Conversely defective regulation of myeloid differentiation has devastating consequences leading to myeloid diseases and disorders such as myeloid aplasia dysplasia and leukemia. (academicediting.org)

We demonstrated the significant eosinophilic growth of leukemic cells in the presence of interleukin-5 (IL-5) in 2 of 15 cases of acute myeloid leukemia. (elsevierpure.com)
Leukemia involves uncontrolled proliferation of specific cells within the immune system (myeloid or lymphoid). (medscape.com)
Myeloproliferative diseases are a heterogeneous group of disorders characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. (medscape.com)
Hematopoietic stem cell transplantation can be considered in young patients with chronic myelogenous leukemia in chronic phase if a human leukocyte antigen (HLA)-matched donor is available. (medscape.com)
Dasatinib (Sprycel): Indicated for the treatment of adult patients with chronic myeloid leukemia in chronic, accelerated, or myeloid or lymphoid blast phase who are resistant or intolerant to prior therapy including imatinib. (medscape.com)
First found to be important in blocking (inhibiting) growth of blood cancer (leukemia) cells, this signaling is also involved in the formation of bone and the development of nerve cells. (medlineplus.gov)
Tomida M. Structural and functional studies on the leukemia inhibitory factor receptor (LIF-R): gene and soluble form of LIF-R, and cytoplasmic domain of LIF-R required for differentiation and growth arrest of myeloid leukemic cells. (medlineplus.gov)
ONUREG is a nucleoside metabolic inhibitor indicated for continued treatment of adult patients with acute myeloid leukemia who achieved first complete remission (CR) or complete remission with incomplete blood count recovery (CRi) following intensive induction chemotherapy and are not able to complete intensive curative therapy ( 1 ). (nih.gov)
It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. (cancerindex.org)
Of the 1,785 seed genes analyzed, 13 prognostic feature genes, including collagen type XXVIII α1 chain (COL28A1), PDS5 cohesin‑associated factor A (PDS5A), zinc‑finger DHHC‑type containing 2 (ZDHHC2), zinc‑finger protein 24 (ZNF24), myosin VA (MYO5A) and myeloid/lymphoid or mixed‑lineage leukemia translocated to 4 (MLLT4), were identified. (cancerindex.org)
Early clinical data in patients with relapsed/refractory IDH2-mutant acute myeloid leukemia show that enasidenib is well tolerated and induces durable complete remissions as a single agent in about 20% of cases. (nih.gov)
The CXXC5 gene encodes a transcriptional activator with a zinc-finger domain, and high expression in human acute myeloid leukemia (AML) cells is associated with adverse prognosis. (oncotarget.com)
2019 ) All-trans retinoic acid (ATRA) induced TFEB expression is required for myeloid differentiation in acute promyelocytic leukemia (APL). (neurotree.org)
The interactions between your cancerous cells of acute myeloid leukemia (AML) as well as the bone marrow (BM) microenvironment have already been postulated to make a difference for resistance to chemotherapy and disease relapse in AML. (bioinf.org)
Not so long ago, therapeutic decisionsin chronic myeloid leukemia(CML) were ratherstraightforward: Allogeneic bonemarrow transplantation was performedin all patients with a donorwho were deemed fit enough for theprocedure, interferon-alfa was administeredto all others able to tolerate it,and the remainder received hydroxyurea.The advent of imatinibmesylate (Gleevec) and, to a lesserdegree, the development of reducedintensityconditioning regimens, haveradically changed the approach totreating CML and have considerablyincreased the complexity of treatmentdecisions. (cancernetwork.com)
Expression of truncated transcripts of the proto-oncogene c-fps/fes in human lymphoma and lymphoid leukemia cell lines. (univ-paris5.fr)
Our results show that NKG2A+ NK cell subsets are the most effective against acute lymphoid leukemia (ALL) targets and represent the most frequent NK cell subsets in the NK cell repertoire. (theses.fr)
In contrast, CD57+ and/or KIR+ NK cell subsets, poorly represented in the NK cell repertoire, were the most effective against acute myeloid leukemia (AML) targets. (theses.fr)
Meanwhile, DHODH inhibitors exert potent differentiation activity in vitro and in vivo in both murine and human models of acute myeloid leukemia (AML). (cancer-research-network.com)
In acute myeloid leukemia (AML), leukemic stem cells (LSCs) underlie mortality but are difficult to isolate due to their low abundance and high similarity to healthy hematopoietic stem cells (HSCs). (nature.com)
Acute myeloid leukemia (AML) serves as a paradigm for the study of cancer stem cells 5 . (nature.com)
11 Moreover, we recently discovered that NPM1 is mutated in about one-third of adult patients with acute myeloid leukemia (AML), 12 which makes NPM1 mutations the most frequent genetic lesions so far identified in de novo AML. (haematologica.org)
Differentiation syndrome is a frequent complication while patients have active acute promyelocytic leukemia (APL). (medscape.com)
But this action should be reserved for patients with a severe condition and severe manifestations of differentiation syndrome in active promyelocytic leukemia. (medscape.com)
The acute myeloid leukemia (AML) patients treated with some agents that can also work through differentiation, like IDH (isocitrate dehydrogenase) inhibitors and FLT3 (fms-like tyrosine kinase 3) inhibitors. (medscape.com)
It should be mentioned that differentiation syndrome in the context of AML is not frequently as severe as compared with acute promyelocytic leukemia. (medscape.com)
In patients with acute myeloid leukemia (AML), treatment with histamine dihydrochloride (HDC) and low-dose IL-2 (HDC/IL-2) in the post-chemotherapy phase has been shown to reduce the incidence of leukemic relapse. (frontiersin.org)
Acute myeloid leukemia (AML) is characterized by unrestrained growth of myeloid cells in bone marrow (BM) and other organs. (frontiersin.org)
Therefore an improved understanding of the molecular mechanisms that control myeloid differentiation will not only provide new insights into fundamental developmental processes but also improve our abilities to treat leukemia and other myeloid disorders. (academicediting.org)
Leukemia cell lines that can be induced to myeloid cells in the presence of chemical inducers such as DMSO and retinoid acid are karyotypically abnormal and thus may not recapitulate the normal myeloid cells. (academicediting.org)
Methods Cell culture W4/129S6 mESCs (Taconic) were plated on γ-irradiated mouse embryonic fibroblasts or 0.1% gelatin-coated 6-well plates and managed in DMEM (high glucose Invitrogen) with 15% FBS 1000 U/mL leukemia inhibitory factor (Chemicon) 0.1 nonessential amino acids 2 l-glutamine 1 sodium pyruvate 10 2 100 Empagliflozin U/mL penicillin and 100 U/mL streptomycin. (academicediting.org)
were as explained previously.5 Briefly EBs were induced from ESC and cultivated for 8 days trypsinized to single cells and coated onto semiconfluent OP9 cells in medium made up of 25 ng/mL oncostatin M 10 ng/mL basic fibroblast growth factor 5 ng/mL IL-6 20 ng/mL SCF 5 ng/mL IL-11 and 1 ng/mL recombinant mouse leukemia inhibitory factor. (academicediting.org)
The success of all-trans retinoic acid (ATRA) therapy for acute promyelocytic leukemia (APL) provides a rationale for using retinoic acid (RA)-based therapy for other subtypes of acute myeloid leukemia (AML). (beatingcancercenter.com)
Preliminary studies suggest that the epigenetic or transcriptional state of leukemia cells determines their susceptibility to ATRA. (beatingcancercenter.com)
AML relapse is caused by a subpopulation of leukemia cells, named leukemic stem cells (LSCs), which are in a different epigenetic state than the total bulk of the AML. (beatingcancercenter.com)
Immune-Onc Therapeutics will conduct a clinical trial for patients with acute myeloid leukemia (AML) and chronic myelomonocytic leukemia (CMML), both of which are types of blood cancer. (ca.gov)
ASXL1 mutation correction by CRISPR/Cas9 restores gene function in leukemia cells and increases survival in mouse xenografts. (ox.ac.uk)
Recurrent somatic mutations of the epigenetic modifier and tumor suppressor ASXL1 are common in myeloid malignancies, including chronic myeloid leukemia (CML), and are associated with poor clinical outcome. (ox.ac.uk)
These results show that the sole correction of a driver mutation in leukemia cells increases survival in vivo in mice. (ox.ac.uk)
This study provides proof-of-concept for driver gene mutation correction via CRISPR/Cas9 technology in human leukemia cells and presents a strategy to illuminate the impact of oncogenic mutations on cellular function and survival. (ox.ac.uk)
Imbalance between differentiation and self-renewal is the root cause of diseases such as acute myeloid leukemia (AML) and other blood cancers. (ufl.edu)
If proper epigenetic regulation is lost, it can lead to some genes being incorrectly marked for activation or not red-flagged for shutoff, disturbing the balance between self-renewal and differentiation, corrupting blood production, and contributing to leukemia development. (ufl.edu)
Her lab studies the epigenetic (or gene regulation) mechanisms that sustain normal blood production and what can go haywire leading to premalignant changes in the HSCs and ultimately to acute myeloid leukemia. (ufl.edu)
One of the first tumors in which a stem cell was identified was acute myeloid leukemia (AML). (nih.gov)
A CD34 positive/CD38 negative cell fraction representing 0.1 to 1 percent of the tumor cells possessed all the leukemia initiating activity in the NOD/SCID transplantation model. (nih.gov)
By contrast, the major fraction of the CD34 positive/ CD38 positive cells and the majority of CD34 negative cells, which comprise most of the cells in the tumor, could not initiate leukemia. (nih.gov)
Nucleophosmin (NPM), a previously characterized nucleolar phosphoprotein, contributes to two other fusion proteins found in lympho-hematopoietic malignancies, anaplastic large cell lymphoma (NPM-ALK) and acute promyelocytic leukemia (NPM-RARalpha). (ustc.edu.cn)
By contrast, the function of the carboxy-terminal fusion partner, myelodysplasia/myeloid leukemia factor 1 (MLF1), is unknown. (ustc.edu.cn)
One of the genes isolated (HLS7) is homologous to the novel human oncogene myeloid leukemia factor 1 (MLF1) involved in the t(3;5)(q25.1;q34) translocation associated with acute myeloid leukemia. (ustc.edu.cn)
3. A yeast two-hybrid screen was conducted to identify binding partners of Mlf1, an oncoprotein recently identified in a translocation with nucleophosmin that causes acute myeloid leukemia. (ustc.edu.cn)
4. Myeloid leukemia factor 1 (MLF1) is a novel oncoprotein involved in translocations associated with acute myeloid leukemia (AML), especially erythroleukemias. (ustc.edu.cn)
A 5-year-old male child was diagnosed with interdigitating dendritic cell sarcoma (IDCS) during his maintenance therapy for B-cell precursor acute lymphoblastic leukemia (B-ALL). (frontiersin.org)
Secondary histiocytic malignancies have been documented following clonal lymphoid proliferations, such as ALL, CLL, follicular lymphoma, hairy cell leukemia, and diffused large B-cell lymphoma ( 4 , 8 , 9 ). (frontiersin.org)
Leukemia Basophilic A rare acute myeloid leukemia in which the immature cells differentiate towards basophils. (nih.gov)
M6 Acute Myeloid Leukemia "An acute myeloid leukemia characterized by a predominant immature erythroid population. (nih.gov)
In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)
The American Cancer Society estimates that in the United States in 2023 there will be about 20,000 new cases of acute myeloid leukemia (AML) and about 11,300 deaths , almost all in adults. (msdmanuals.com)
acute myeloid leukemia is caused by a series of acquired genetic aberrations. (msdmanuals.com)
Acute myeloid leukemia has a number of subtypes and precursor neoplasms that are distinguished from each other by morphology, immunophenotype, cytochemistry, and genetic abnormalities (see also The 2016 World Health Organization [WHO] Classification of myeloid neoplasms ) all of which have important implications for prognosis and treatment. (msdmanuals.com)
RUNX1-positive leukemia cells in preclinical models. (lu.se)

Benzene metabolite's enhancement of growth of myeloid cells through an active oxygen mechanism may be involved in a number of aspects of benzene toxicity, including enhanced granulocytic growth and differentiation, stimulation of GM-CSF-induced colony formation, apoptosis inhibition, and stimulation of progenitor cell mitogenesis in the bone marrow. (nih.gov)
However, as the results vary, more study is required to explore the prospect of CXCR4 inhibitors to induce cell loss of life through apoptosis. (bioinf.org)
They discovered that ulocuplumab displays antitumor activity in set up tumors including subcutaneous xenograft types of APL and induces apoptosis on the -panel of cell lines including AML. (bioinf.org)
Furthermore, DHODH can connect the bioenergy of mitochondria, cell proliferation, ROS production, and apoptosis of some cell types. (cancer-research-network.com)
Extracellular release of ROS from myeloid cells may also trigger dysfunction and apoptosis of adjacent cells, including elements of lymphocyte-mediated immunity ( 14 ). (frontiersin.org)
By this mechanism, HDC safeguards natural killer cells and cytotoxic T cells from apoptosis inflicted by neighboring ROS-producing myeloid cells, thus facilitating immune-mediated elimination of malignant cells ( 14 , 16 - 18 ). (frontiersin.org)
Recently, several studies showed that ATRA may drive leukemic cells efficiently into differentiation and/or apoptosis in a subset of AML patients with an NPM1 mutation, a FLT3-ITD, an IDH1 mutation, and patients overexpressing EVI-1. (beatingcancercenter.com)
Searching for response biomarkers might also reveal novel RA-based combination therapies with an efficient differentiation/apoptosis-inducing effect in non-APL AML patients. (beatingcancercenter.com)
it regulates the production of hematopoietic cells by stimulating cell cycle progression, differentiation, and inhibition of apoptosis. (nsdfu.org)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)

13. Transfection of rhabdomyosarcoma cells with connexin43 induces myogenic differentiation. (nih.gov)
2]The authors comment that we stilldo not understand 'precisely how[Bcr-Abl] induces the leukemic phenotype. (cancernetwork.com)
It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. (bvsalud.org)
LIF inhibits differentiation in normal stem cells, but in leukemic myeloid cells, it induces differentiation thereby preventing further growth. (bio-rad-antibodies.com)
Reduced amount of PU.1 expression by 80%C100% induces AML in mice, whereas PU.1 halpoinsufficiency causes subtle adjustments in hematopoietic differentiation, but isn't sufficient to induce leukemia3, 9, 11, 13, 14. (exatecan-mesylate.com)

We are seeking a postdoc for the Wennerberg group to study drug responses of leukemic stem and progenitor cells from patients with acute myeloid leukaemia using single cell approaches. (biologists.com)
The purpose of this initiative is to encourage research aimed at delineating the function and regulation of homing determinants and their receptors (or ligands) that are involved in regulation of growth and differentiation of hematopoietic stem and progenitor cells. (nih.gov)
This initiative, Homing Determinants in Hematopoietic Stem and Progenitor Cells, is related to the priority areas of maternal and infant health and cancer. (nih.gov)
PU.1 expression in hematopoietic multipotent stem and progenitor cells sorted from UREhet mice exhibited a significant ( 0.05), but very modest reduction of expression compared to wild type (WT) littermates (37 8% in Lin?Sca-1+cKit+ (LSK) cells, 33% 4% in common myeloid progenitors (CMP), and 26% 20% in granulocytic/monocytic progenitors (GMP)) (Fig. 1a and Supplementary Fig. 1a,b). (exatecan-mesylate.com)

None of the geneticlesions associated with blast crisis, exceptrearrangements affecting the EVI-1transcription factor,[3] explain the moststriking feature of blast crisis, which isthe loss of terminal differentiation. (cancernetwork.com)

We used several assays to determine the effects of benzene metabolites (hydroquinone, benzenetriol, benzoquinone) and active oxygen species (xanthine/xanthine oxidase) on cell growth and cell cycle kinetics of the human myeloid cell line HL-60. (nih.gov)
Significantly reduced cell growth and increased myeloid differentiation were observed in ASXL1 mutation-corrected cells, providing new insights into the role of ASXL1 in human myeloid cell differentiation. (ox.ac.uk)

Nonetheless, the anti-leukemic activity of DHODH inhibitors points toward a novel link between uridine biosynthesis and cell-fate decisions. (cancer-research-network.com)
Details regarding the mechanisms of anti-leukemic activity of HDC/IL-2 have only partially been explored, in particular regarding the contribution by HDC ( 8 , 9 ). (frontiersin.org)

It is currently unknown what effect immune-stimulating dietary supplements may have on proliferation and differentiation of hematopoietic cells in these patients. (medscape.com)
Proliferation and differentiation of myeloid cells and Regulation of myeloid transcription factors and other proteins in hematopoiesis and their dysregulation in leukemic cancers. (icsccb.org)
Basics of Carbohydrates responsible for the proliferation and differentiation of hematopoietic progenitor cells in the bone marrow Bone marrow The soft tissue filling the cavities of bones. (lecturio.com)
Pharmacologic erythropoietin Erythropoietin Glycoprotein hormone, secreted chiefly by the kidney in the adult and the liver in the fetus, that acts on erythroid stem cells of the bone marrow to stimulate proliferation and differentiation. (lecturio.com)
The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. (lecturio.com)

It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells. (biovendor.com)
2019 ) Inhibition of UBE2L6 attenuates ISGylation and impedes ATRA-induced differentiation of leukemic cells. (neurotree.org)
A number of different systems of CXCR4 inhibition in charge of antileukemia results have been discovered: physical mobilization results, reduced prosurvival signaling via CXCL12-CXCR4 downstream signaling (AKT and CD244 MAPK pathways), the induction of differentiation, results on BCL-XL via the CXCR4/YY1/allow-7a axis (also on non-mobilized AML cells), as well as the activation of ADCC and/or CDC regarding anti-CXCR4 monoclonal antibodies. (bioinf.org)
Mediates inhibition of natural killer cells cytotoxicity. (nih.gov)

Hematologic malignancies are more profoundly immunosuppressive secondary to the clonal expansion of nonfunctional, immune, cancer cells (T or B lymphocytes). (medscape.com)
The team at GT Biopharma intends to study GTB-3550, an anti-CD16-IL-15-anti-CD33 tri-specific killer engager, or TriKE, in CD33 positive leukemias, a marker expressed on tumor cells in AML, myelodysplastic syndrome, or MDS, and other hematopoietic malignancies. (adcreview.com)
Recent data from both hematologic malignancies and solid tumors have suggested that there are only minor populations of cells in each malignancy that are capable of tumor initiation. (nih.gov)
Currently, tumor stem cells have been isolated and characterized in several hematologic malignancies and some solid tumors. (nih.gov)

Bone marrow examination revealed the rather high percentages (6% and 9%) of atypical eosinophils in the total nucleated bone marrow cells in these two cases. (elsevierpure.com)
In the remaining 13 cases, eosinophils were less than 2% in the nucleated bone marrow cells. (elsevierpure.com)
Our findings suggest that atypical eosinophils observed in the bone marrow were derived from the leukemic clone in two cases of AML. (elsevierpure.com)
[ 89 ] The use of myeloid growth factors after chemotherapy to facilitate bone marrow recovery has become common-place in some aspects of cancer treatment. (medscape.com)
Such studies will undoubtedly have a major impact on our ability to identify the factors that are involved in such critical functions such as stem cell self-renewal, maintenance of progenitor cells, bone marrow and stem cell engraftment, and graft maintenance and rejection. (nih.gov)
Clinical characteristics at diagnosis were collected, including peripheral white blood cell (WBC) counts, blast percentages in peripheral blood (PB) and bone marrow (BM), French-American-British (FAB) subtypes, cytogenetic risk group, and frequencies of common recurrent genetic mutations. (researchsquare.com)
Another unmet medical need is observed in patients with Myelodysplastic Syndrome or MDS, a series of conditions that can occur when the blood-forming cells in the bone marrow become abnormal, leading to low numbers of one or more types of blood cells. (adcreview.com)
Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells. (lecturio.com)
Main myeloid progenitors isolated from bone marrows are physiologic but they are generally Empagliflozin of limited quantities hard to purify to homogeneity refractory to genetic manipulations and not suited for long-term culture 4 thus Empagliflozin limiting their applications. (academicediting.org)
HEK293T cells and OP9 bone marrow stromal cells were purchased from ATCC and were cultured following ATCC's recommendations. (academicediting.org)
Our findings indicate that chondro cytes had not just differentiated in direction of osteoblast like cells, but also finished the differentiation to cells that had been capable of making mineralized bone matrix. (inhibitorkit.com)
By producing over 2 million new mature blood cells every second, the bone marrow, which is the home of the hematopoietic system, is the hardest working organ in the human body. (ufl.edu)
Normal blood production starts with the hematopoietic stem cells (HSCs), specialized cells in the bone marrow that make mature cells of the blood in the process called differentiation. (ufl.edu)
These entities are now considered as "true" hematopoietic tumors arising from bone marrow precursors, together with an L-type histiocytosis, Langerhans cell histiocytosis (LCH), and Erdheim-Chester disease (ECD), while follicular dendritic cell sarcoma shows a different molecular signature similar to the sarcomas of mesenchymal origin ( 4 , 5 ). (frontiersin.org)

Although IL-5 acts on normal eosinophil committed precursors as a lineage-specific growth factor, at least some leukemic cells reacted to IL-5 and could proliferate and differentiate along eosinophilic pathway. (elsevierpure.com)
Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). (nih.gov)
Empagliflozin Two in vitro experimental models (primary normal myeloid precursors and leukemic cells arrested at numerous developmental stages) have been utilized for the studies of myeloid differentiation. (academicediting.org)

2020 ) Mutations in long-lived epithelial stem cells and their clonal progeny in pre-malignant lesions and in oral squamous cell carcinoma. (neurotree.org)
In 10-20% of healthy individuals over age 70, the acquisition of pre-leukemic mutations in hematopoietic stem cells (HSCs) results in the dominance of a small number of HSC-derived clones, a process termed Clonal Hematopoiesis of Indeterminate Potential (CHIP) 6 , 7 . (nature.com)
Fig. 1: MutaSeq for high-quality single-cell RNA-seq data with clonal information. (nature.com)
Comprehensive genomic profiling of the formalin fixed the tumor tissue, and the cryopreserved leukemic cells revealed several common alterations and divergent clonal evolution with a novel MAP2K1 mutation of the IDCS, which is responsible for the trans-differentiation of the common lymphoid-committed tumor progenitor. (frontiersin.org)

Leukemic cells also differentiated to neutrophils or both neutrophils and eosinophils in response to granulocyte colony-stimulating factor or interleukin-3, respectively, but did not respond noticeably to granulocyte-macrophage colony-stimulating factor. (elsevierpure.com)
It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. (fishersci.com)
The ESC-derived immortalized progenitor cells demonstrate normal karyotyping are genetically manipulatable and can be differentiated into functional neutrophils. (academicediting.org)

This molecule is expressed by monocytic/myeloid lineage cells. (fishersci.com)

CD19 has been targeted through genetically engineered chimeric-antigen receptor (CAR) T cells, and via bispecific CD3-CD19 antibodies, with reports of relapse via CD19 loss. (nih.gov)
Our data demonstrates plasticity of leukemic cells under antigen-specific immune pressure, with de-differentiation and lineage switch. (nih.gov)
The Salmonella enterica effector SteD depletes mature MHC class II (mMHCII) molecules from the surface of infected antigen-presenting cells through ubiquitination of the cytoplasmic tail of the mMHCII β chain. (academic-accelerator.com)
6028 Background: Eftilagimod alpha (efti) is a soluble LAG-3 protein that binds to a subset of MHC class II molecules to mediate antigen presenting cell (APC) activation and CD8 T-cell activation. (academic-accelerator.com)

Interdigitating dendritic cell sarcoma (IDCS) is an exceedingly rare dendritic cell neoplasm with even less frequent occurrence among children ( 1 , 2 ). (frontiersin.org)
While most of the cases are primary tumors, IDCS may also present as secondary histiocytosis, which term applies to cases where histiocytic/dendritic cell tumors follow or appear simultaneously with another hematopoietic tumor. (frontiersin.org)

In addition, development of nerve cells, particularly those involved in the autonomic nervous system, is abnormal, leading to the problems with breathing, feeding, and regulating body temperature characteristic of this condition. (medlineplus.gov)
Understanding the process of myeloid differentiation offers important insights into both normal and abnormal developmental processes but is limited by the dearth of experimental models. (academicediting.org)

16. Up-regulation of type XIX collagen in rhabdomyosarcoma cells accompanies myogenic differentiation. (nih.gov)
Later relapses had loss of all the B cell surface markers, along with down regulation of B cell transcripts (such as EBF1 and PAX5) in the mRNA. (nih.gov)
Immune modulation group has an interest in understanding the mechanisms of lysophosphatidic acid (LPA) in immune cell regulation and cancer. (ils.res.in)
The polycomb genes have an essential role in embryogenesis, regulation of the cell cycle, and lymphopoieisis. (nih.gov)

In addition, changes in gene expression in lys-gfp-er-hoxa9 cells with ML390 for 12, 36, and 72 hours were similar to primary neutrophil differentiation. (cancer-research-network.com)

While mutational status discriminates between healthy and cancerous cells, gene expression distinguishes stem cells and progenitor cell populations. (nature.com)

Caution should also be used when patients are receiving therapy with colony-stimulating factors pegfilgrastim and filgrastim, because of the presence of the granulocyte colony-stimulating-factor receptor target of pegfilgrastim and filgrastim on tumor cell lines, including some myeloid, T-lymphoid, lung, head and neck, and bladder tumor cell lines. (medscape.com)
This receptor spans the cell membrane, which allows it to attach (bind) to other proteins, called ligands, outside the cell and send signals inside the cell that help the cell respond to its environment. (medlineplus.gov)
Finally, CXXC5 knockdown in human AML cell lines caused significantly increased expression of the potential tumor suppressor gene TSC22 and genes encoding the growth factor receptor KIT, the cytokine Angiopoietin 1 and the selenium-containing glycoprotein Selenoprotein P. Thus, high CXXC5 expression seems to affect several steps in human leukemogenesis, including intracellular events as well as extracellular communication. (oncotarget.com)
A major aspect of the program is to encourage research on the stem cell homing receptor or receptors and methods to manipulate receptor expression and binding. (nih.gov)
Not surprisingly, thetranscriptional profile of Bcr-Abl-transformed hematopoietic cells is distinctfrom that of platelet-derivedgrowth factor receptor-beta-transformedcells. (cancernetwork.com)
Also referred to as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC3, gp67, p67), CD33 is a transmembrane receptor expressed on cells of myeloid lineage. (adcreview.com)
KIT is a receptor tyrosine kinase type III, which binds to stem cell factor. (inhibitorkit.com)
BCG can activate the pattern recognition receptor (PRR) on the surface of alveolar epithelial cells. (ijbs.com)

Subsequently, many groups explored if the US Meals and Medication Administration (FDA)-authorized little molecular CXCR4 inhibitor, plerixafor (AML3100), affected the trafficking and success of AML cells and and data exposed that LY2510924 at nanomolar concentrations quickly and durably disrupts the CXCL12-CXCR4 axis in AML cells, which inhibits proliferation of AML cells instead of causing cell loss of life (as opposed to BKT140 data). (bioinf.org)
In vitro, treatment of Lys-GFP-ER-HoxA9 cells with ML390 for 48 hr inhibits DHODH activity. (cancer-research-network.com)
Prostaglandin E2 inhibits the synthesis of G-CSF, while in epithelial, endothelial, and fibroblastic cells, secretion of G-CSF is induced by IL-17. (rockland.com)

They are positive for the neural stem cell marker CD133 and have a marked capacity for self renewal and differentiation. (nih.gov)

In low concentrations, benzene and its metabolite hydroquinone are known to have diverse biological effects on cells, including the synergistic stimulation with GM-CSF of hematopoietic colony formation in vitro, stimulation of granulocytic differentiation in vitro and in vivo, and general suppression of hematopoiesis in vivo. (nih.gov)

Murine CD19 CAR treatment of ALL-bearing mice resulted in long-term remission with long persistence of T cells. (nih.gov)
Classic chemotherapy regimens primarily target actively cycling "blast" cells and initially lead to remission. (nature.com)
The initial treatment comprises induction and consolidation chemotherapy aimed at inducing and sustaining the disappearance of leukemic cells (complete remission, CR) ( 1 ). (frontiersin.org)
Treatment includes induction chemotherapy to achieve remission and postremission chemotherapy (with or without stem cell transplantation) to avoid relapse. (msdmanuals.com)

LIFR signaling can control several cellular processes, including growth and division (proliferation), maturation (differentiation), and survival. (medlineplus.gov)
Re-introduction of miR-155 into HL-60 cells restored monocytic maturation and repressed cell proliferation. (molcells.org)
Here, we report that human NOX2 + myelomonocytic/monocytic AML cell lines showed increased expression of maturation markers along with reduced leukemic cell proliferation after exposure to HDC in vitro . (frontiersin.org)
In contrast, HLS7 promoted maturation of M1 monoblastoid cells and increased myeloid colony formation in vitro. (ustc.edu.cn)
Ectopic expression of Madm in M1 myeloid cells suppressed cytokine-induced differentiation unlike Mlf1, which promotes maturation. (ustc.edu.cn)
In this study, we demonstrate that ectopic expression of Mlf1 prevented J2E erythroleukemic cells from undergoing biological and morphological maturation in response to erythropoietin (Epo). (ustc.edu.cn)

No matter if the suggested trans chondroid ossification is trans differentiation like a sudden switch from the chon drogenic to your osteogenic phenotype or maybe a continuous differentiation was not assessed within this experiment. (inhibitorkit.com)
In addition to isolating and characterizing tumor stem cells themselves, it is also important to identify the genes and proteins that facilitate the self renewal phenotype that characterize all stem cells. (nih.gov)
Two families of proteins related to self renewal, the polycomb gene Bmi-1 and the Wnt signaling pathway proteins, have been related to the maintenance of the tumor stem cell phenotype. (nih.gov)
2. Hemopoietic lineage switching occurs when leukemic cells, apparently committed to one lineage, change and display the phenotype of another pathway. (ustc.edu.cn)
Enforced expression of HLS7 in J2E cells induced a monoblastoid phenotype, thereby recapitulating the spontaneous erythroid to myeloid lineage switch. (ustc.edu.cn)

Furthermore, we have investigated the influence of KIR/HLA interactions on Antibody Dependant Cellular Cytotoxicity (ADCC) modulation, showing that the low number of KIR ligands in lymphoma patients favors a good rituximab-dependent NK cell responses. (theses.fr)
NPM-ALK indentifies a new category of T/Null lymphomas with distinctive molecular and clinicopathological features, that is going to be included as a novel disease entity (ALK+ anaplastic large cell lymphoma) in the new WHO classification of lymphoid neoplasms. (haematologica.org)

By gain- and loss-of-function analysis, we determined that CCAT1 repressed monocytic differentiation and promoted cell growth of HL-60 by sequestering tumor suppressive miR-155. (molcells.org)
Determine the safety, pharmacokinetics (PK), pharmacodynamics (PD), and preliminary efficacy of IO-202 as monotherapy in patients with AML with monocytic differentiation and CMML refractory to or relapsed after available therapies known to be active in their disease. (ca.gov)

2019 ) Retinoids induce antagonism between FOXO3A and FOXM1 transcription factors in human oral squamous cell carcinoma (OSCC) cells. (neurotree.org)
They can induce differentiation of leukemic cells in AML. (medscape.com)

The initiative will identify the cascade of interactive events in early phases of hematopoietic cell differentiation and its relation to engraftment. (nih.gov)

In addition, post hoc analyses of phase III trial results suggested that HDC/IL-2 may be preferentially or specifically efficacious in patients where the leukemic clone is dominated by monocytic cells, i.e., myelomonocytic (FAB class M4) or monocytic (M5) AML ( 7 ). (frontiersin.org)

LDHB was aberrantly overexpressed in human AML peripheral blood mononuclear cell (PBMC) and AML cell lines. (nih.gov)
Peripheral smear of a patient with agnogenic myeloid metaplasia (myelofibrosis) shows leukoerythroblastosis. (medscape.com)
A) Peripheral blood smear showing many hypochromic and microcytic cells. (haematologica.org)
The protein is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. (lecturio.com)

Furthermore, NOX2-derived ROS were recently found to facilitate mitochondrial transfer from BM stromal cells to AML cells, which may enhance metabolism and survival of AML cells ( 13 ). (frontiersin.org)
Leukemic stem cells group is investigating the mechanisms responsible for the survival of the lineage negative CD34+ leukemic stem cells against tyrosine kinase inhibitors. (ils.res.in)
The survival of LSCs after therapy is the main cause of the poor prognosis of AML patients, and novel differentiation therapies should drive these LSCs into maturity. (beatingcancercenter.com)
Mice xenografted with mutation-corrected KBM5 cells showed significantly longer survival than uncorrected xenografts. (ox.ac.uk)
The mechanisms, by which these alter cell function and survival, are outlined below. (ukaachen.de)
Physiological ABL1 codes for a tyrosine kinase involved in cell proliferation, differentiation, and survival. (ukaachen.de)

While such pre-leukemic stem cells (pre-LSCs) are capable of giving rise to healthy blood and immune cells, additional mutations can cause a complete block in differentiation and thereby result in the malignant expansion of aberrant progenitor cells 8 . (nature.com)
Based on the recent WHO classification, this entity belongs to the malignant histiocytosis group that also includes histiocytic, Langerhans cell (HS, LCS), and indeterminate cell sarcomas ( 3 ). (frontiersin.org)
Acinic Cell Carcinoma A malignant glandular epithelial neoplasm consisting of secretory cells forming acinar patterns. (nih.gov)
Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)

This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. (cancerindex.org)
Fps/Fes protein-tyrosine kinase regulates mast cell adhesion and migration downstream of Kit and beta1 integrin receptors. (univ-paris5.fr)
Under native conditions, both in resting and proliferating cells, over 95% of cellular NPM protein exists as an oligomer. (haematologica.org)
We have used the CRISPR/Cas9 system to correct the ASXL1 homozygous nonsense mutation present in the CML cell line KBM5, which lacks ASXL1 protein expression. (ox.ac.uk)

However, detailed analyses of molecular pathways involved in the trans-differentiation process are lacking. (frontiersin.org)
Here, we present a secondary pediatric IDCS case featuring an activating MAP2K1 mutation, possibly driving the trans-differentiation of the common lymphoid-committed tumor progenitor. (frontiersin.org)

These genes performed well on sample classification and prognostic risk differentiation, and six pathways, including adherens junction, cyclic adenosine 3',5'‑monophosphate signaling and Ras signaling pathways, were enriched for these feature genes. (cancerindex.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Particularly, ML390 is an inducer of myeloid differentiation and causes myeloid differentiation in murine (ER-HoxA9) and human (U937 and THP1) AML models. (cancer-research-network.com)

[ 86 ] The sargramostim prescribing information contains similar precautions regarding tumor cell growth and the need to exercise caution, particularly in cancers with myeloid characteristics. (medscape.com)
[ 88 ] While these effects may clearly be detrimental to an untreated patient, many investigators have attempted to exploit this effect by "priming" tumor cells with a growth factor (e.g., filgrastim) and then immediately administering cytotoxic chemotherapy. (medscape.com)
Cayphas S, Van Damme J, Vink A, Simpson RJ, Billiau A, Van Snick J. Identification of an interleukin HP1-like plasmacytoma growth factor produced by L cells in response to viral infection. (biovendor.com)
Enhancement of myeloid cell growth by benzene metabolites via the production of active oxygen species. (nih.gov)
HL-60 cells treated with these chemicals for 2 h in PBS showed increased growth over untreated controls in a subsequent 18h growth period in complete media. (nih.gov)
Catalase treatment abrogated the increased cell growth of all chemicals, suggesting an oxidative mechanism for the effect of all treatments alike. (nih.gov)
Cell cycle kinetics assays showed that the growth increase was caused by an increased recruitment of cells from G0/G1 to S-phase for both hydroquinone and active oxygen, rather than a decrease in the length of the cell cycle. (nih.gov)
Therefore, repressing cell growth or overcoming differentiation arrest by manipulating such key regulators becomes the major driving force of this blood cancer. (molcells.org)
The consumption of DHODH also increased ROS production, decreased membrane potential, and delayed cell growth. (cancer-research-network.com)
After 3-day growth the progenitor cells were transferred onto new semiconfluent OP9 cells and cultured in. (academicediting.org)
Additionally, reduction of cell integrity via cell proliferation was prominent in the border amongst the osteoblastic growth zone along with the chondrocytic areas while in the arch centra and in interverte bral room. (inhibitorkit.com)
SC.912.L.16.8 Explain the relationship between mutation, cell cycle, and uncontrolled cell growth potentially resulting in cancer. (ufl.edu)
Acidophil Stem Cell Pituitary Neuroendocrine Tumor/Adenoma "An infrequent pituitary neuroendocrine tumor composed of cells showing immunoreactivity for prolactin and less often growth hormone. (nih.gov)

Oncogenic mutations in these cells can cause cancers that maintain a hierarchical organization reminiscent of the tissue of origin. (nature.com)

First of all, we need to mention that the diagnosis of differentiation syndrome is based on the clinical symptoms and signs. (medscape.com)
The clinical benefit of HDC/IL-2 is pronounced in monocytic forms of AML, where the leukemic cells express histamine type 2 receptors (H 2 R) and the NAPDH oxidase-2 (NOX2). (frontiersin.org)
The ability of HDC to protect immune cells with tumor-killing capacity has been proposed to contribute to the clinical benefit of HDC-based therapy in AML ( 8 , 19 , 20 ). (frontiersin.org)

The proteins involved in self renewal in embryonic and adult stem cells appear to be subverted in tumorigenesis to allow the tumor-initiating cells to maintain self renewal capacity. (nih.gov)

The cytotoxicity of cisplatin correlates with cellular arrest in the G2 phase of the cell cycle. (lookformedical.com)

It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. (adcreview.com)

cDNA representational difference analysis was used to identify myeloid-specific genes that may be associated with an erythroid to myeloid lineage switch involving the murine J2E erythroleukemic cell line. (ustc.edu.cn)
These data show that HLS7 can influence erythroid/myeloid lineage switching and the development of normal hemopoietic cells. (ustc.edu.cn)

The postdoc will combine the drug response information with other molecular profiling with the goal of discovering new approaches to treat individual or targeted groups of leukemic patients. (biologists.com)
T cell-replete haplo-identical Hematopoietic Stem Cell Transplantation (HSCT), using Post-transplant Cyclophosphamide (PTCy) as a treatment for Graft-versus- Host-Disease (GvHD), constitute a potential curative treatment for leukemic patients without identical HLA donor. (theses.fr)
Overexpression of microRNA-93 (miR-93) predicted worse outcome in non-small cell lung cancer (NSCLC) and gastric cancer patients, yet the prognostic role of miR-93 in AML is still unclear. (researchsquare.com)
Moreover, the expression levels of miR-93 was no association with either EFS or OS in patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). (researchsquare.com)
And we can have some patients, depending between 3% with FLT3 inhibitors and up to 15% with IDH inhibitors, that are manifesting differentiation syndrome. (medscape.com)
However, most AML patients will experience life-threatening relapses in the post-chemotherapy phase, likely as the result of expansion of residual leukemic cells, and few treatments are available to prevent relapse ( 2 , 3 ). (frontiersin.org)
The team will treat AML and CMML patients with an antibody therapy called IO-202 that targets leukemic stem cells. (ca.gov)
Patients with ETP-ALL were defined as described previously.6 Additional details regarding the study group are provided in the non-leukemic events). (nsdfu.org)
symptoms of extramedullary leukemic infiltration are present in only about 5% of patients (often as skin manifestations). (msdmanuals.com)

4. Genomic imbalances in rhabdomyosarcoma cell lines affect expression of genes frequently altered in primary tumors: an approach to identify candidate genes involved in tumor development. (nih.gov)
We also observed that exposure to HDC altered the expression of genes involved in differentiation and cell cycle progression in AML cells and that these effects required the presence of NOX2. (frontiersin.org)

A shift in NP cell population coincides with spinal problems like IDD and adjustments inside the synthesis of matrix molecules vary together with the degree of degeneration. (inhibitorkit.com)

6 , 7 Distinctive laboratory features are microcytic anemia with hypochromic red cells, increased red cell distribution width and evidence of parenchymal iron overload: for a conclusive diagnosis of XLSA, however, identification of the ALAS2 mutation is required. (haematologica.org)

Colon cancer-associated transcript-1 ( CCAT1 ) gene encodes an lncRNA whose over-activation was observed in an expanding list of primary human solid tumors and tumor cell lines, however its biological roles in acute myeloid leukaemia (AML) has not been reported yet at present. (molcells.org)
Two theories have been developed to account for the observation that apparently not every tumor cell is a tumor initiating cell (T-IC). (nih.gov)

However, the role of NK cells in this modality of transplantation remains poorly understood. (theses.fr)
Transplantation of these putative neural tumor stem cells into the forebrains of NOD/SCID mice yields tumors phenotypically resembling the tumors from which the stem cells were isolated. (nih.gov)

The tumor initiating capacity of the cells was again verified in an in vivo NOD/SCID engraftment assay. (nih.gov)

2020 ) Doxycycline-induced exogenous Bmi-1 expression enhances tumor formation in a murine model of oral squamous cell carcinoma. (neurotree.org)
Oral cancer group has interest on understanding and targeting chemoresistance in Oral Squamous Cell Carcinoma (OSCC). (ils.res.in)

Down regulated genes included genes relevant to blood cell synthesis and mitochondrial perform. (inhibitorkit.com)

Expression studies as well as gene silencing experiments suggest that CXXC5 is important in normal myelopoiesis [ 2 ] and for differentiation of endothelial cells [ 3 ]. (oncotarget.com)
Tumor angiogenesis group is interested in understanding the mechanisms of endothelial gene expression during tumor angiogenesis using human endothelial cells and zebrafish as a model. (ils.res.in)

Anatomy42

Organisms8

Diseases29

Chemicals and Drugs100

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes51

Disciplines and Occupations2

Information Science3

Blasts12

Macrophages6

Leukemia60

Apoptosis10

Induces5

Stem and progenitor4

Terminal differentiation1

Human myeloid cell2

Anti-leukemic activity2

Proliferation and differentiation5

Inhibition4

Malignancies4

Bone14

Precursors3

Clonal4

Neutrophils3

Expressed by monocytic1

Antigen4

Dendritic cell2

Abnormal2

Regulation4

Neutrophil differentiation1

Progenitor cell1

Receptor8

Inhibits3

Self-renewal and differentiation1

Vitro and in vivo1

Remission4

Maturation6

Phenotype5

Lymphoma2

Monocytic differentiation2

Induce2

Hematopoietic cell1

Myelomonocytic1

Peripheral4

Survival6

Malignant4

Protein4

Trans differentiation2

Pathways2

Inducer1

Growth13

Cancers1

Clinical3

Proteins1

Arrest1

Lymphoid cells1

Erythroid2

Patients9

Expression of ge2

Molecules1

Mutation1

Tumor cell2

Transplantation2

Engraftment1

Squamous cell carc2

Genes1

Endothelial cells2