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  • kinase
  • Execution of cell death, but not induction of autophagy, was strictly dependent on expression of receptor-interacting protein (RIP-1) kinase and cylindromatosis (turban tumor syndrome) (CYLD), two key regulators of necroptosis. (jci.org)
  • resistance
  • In vivo resistance to first-line chemotherapy, including to glucocorticoids, is a strong predictor of poor outcome in children with acute lymphoblastic leukemia (ALL). (jci.org)
  • Modulation of cell death regulators represents an attractive strategy for subverting such drug resistance. (jci.org)
  • Consistent with a protective role for mTOR in glucocorticoid resistance in childhood ALL, combination of rapamycin with the glucocorticoid dexamethasone triggered autophagy-dependent cell death, with characteristic features of necroptosis. (jci.org)
  • Accordingly, both inhibition of RIP-1 and interference with CYLD restored glucocorticoid resistance completely. (jci.org)
  • progression
  • E ) Mcl-1 ubiquitination was analyzed by anti-Mcl-1 co-IP and Western blot using anti-HA antibody following transfection with HA-tagged ubiquitin (HA-Ub) constructs during cell cycle progression. (jci.org)
  • F ) Mitochondrial and nuclear fractions were isolated from cells at various time points during cell cycle progression. (jci.org)