• Myeloid cell Nuclear Differentiation Antigen is a protein that in humans is encoded as MNDA gene. (wikipedia.org)
  • The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. (wikipedia.org)
  • Expression of MY7 antigen on myeloid precursor cells. (bdbiosciences.com)
  • 2014) Purification, characterization and docking studies of the HIN domain of human myeloid nuclear differentiation antigen (MNDA). (guidetopharmacology.org)
  • Absent in melanoma (AIM)-like receptors (ALRs): myeloid cell nuclear differentiation antigen. (guidetopharmacology.org)
  • Herein, we report a potential leukocyte differentiation antigen gene VSTM1 ( V-set and transmembrane domain-containing 1 ) that was downregulated in bone marrow cells from leukemia patients and exhibited a higher degree of promoter methylation. (biomedcentral.com)
  • Therefore, VSTM1-v1 might represent an important myeloid leukocyte differentiation antigen and provide a potential target for the diagnosis and treatment of leukemia. (biomedcentral.com)
  • VSTM1 ( V-set and transmembrane domain-containing 1 ) encodes a potential leukocyte differentiation antigen that is highly expressed in myeloid cells, but silenced in multiple leukemia cell lines [ 1 ]. (biomedcentral.com)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • Ly6C high MC presented downregulated co-stimulatory receptors (CD2, GITR, and TIM1) which direct immune cell proliferation, and upregulated co-stimulatory ligands (LIGHT and SEMA4A) which trigger antigen priming and differentiation. (frontiersin.org)
  • This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. (biolegend.com)
  • Dendritic cells (DCs) are specialized antigen-presenting cells that have a notable role in the initiation and regulation of innate and adaptive immune responses. (mdpi.com)
  • Conventional dendritic cells (cDCs), also known as myeloid dendritic cells or classical dendritic cells, are a type of antigen-presenting cell (APC), cDCs originate from hematopoietic stem cells, specifically from the common myeloid progenitor in the bone marrow. (origene.com)
  • The CD117 (c-Kit) antigen is the cell-surface receptor for stem cell factor (SCF). (southernbiotech.com)
  • Demonstration that human mast cells arise from a progenitor cell population that is CD34(+), c-kit(+), and expresses aminopeptidase N (CD13). (bdbiosciences.com)
  • In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
  • We investigated the origin of SF3B1 mutations within the bone marrow hematopoietic stem and progenitor cell compartments in patients with MDS-RS. (nih.gov)
  • All recurrent mutations identified in mononuclear cells could be tracked back to the phenotypically defined hematopoietic stem cell (HSC) compartment in all investigated patients and were also present in downstream myeloid and erythroid progenitor cells. (nih.gov)
  • While in agreement with previous studies, little or no evidence for clonal ( SF3B1 mutation) involvement could be found in mature B cells, consistent involvement at the pro-B-cell progenitor stage was established, providing definitive evidence for SF3B1 mutations targeting lymphomyeloid HSCs and compatible with mutated SF3B1 negatively affecting lymphoid development. (nih.gov)
  • Assessment of stem cell function in vitro as well as in vivo established that only HSCs and not investigated progenitor populations could propagate the SF3B1 mutated clone. (nih.gov)
  • Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
  • Moreover, the CD34 + CD38 - progenitor cells expressed variable amounts of the target receptor CD33, CD133 and c-kit (CD117) [ 20 ]. (researchsquare.com)
  • Ectopic expression of AML1-MTG8 in L-G murine myeloid progenitor cells inhibits differentiation to mature neutrophils and induces cell proliferation in response to granulocyte colony-stimulating factor (G-CSF). (embl-heidelberg.de)
  • According to the classical model, it proposes a common myeloid progenitor (CMP) as the precursor of both monocytes/macrophages and DCs. (origene.com)
  • Signaling through SCF/c-Kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells. (southernbiotech.com)
  • A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. (southernbiotech.com)
  • For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. (southernbiotech.com)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • MSCs have a lower ability to differentiate into osteoblasts but a higher ability to differentiate into adipocytes, which could be linked to myeloid malignancies ( Woods and Guezguez, 2021 ). (techscience.com)
  • Acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) are myeloid blood cell malignancies that show great heterogeneity. (biomedcentral.com)
  • These malignancies are further characterized by the maturity and differentiation of the individual cell types and are divided into acute leukemias such as acute myeloid leukemia and acute lymphoblastic leukemia and chronic leukemias such as chronic myeloid leukemia and chronic lymphocytic leukemia. (medscape.com)
  • We examined the CD45 dim CD34 + CD38 - CD133 + cells on bone marrow samples of hematologic malignancies and healthy controls using four-color flow cytometry experiments. (researchsquare.com)
  • The functional diversity of cells that compose myeloid malignancies, i.e., the respective roles of genetic and epigenetic heterogeneity in this diversity, remains poorly understood. (philinbiomed.org)
  • The present study demonstrated the biological and functional evidence for the critical role of RUNX1 -MT in ASXL1 -mutated leukemia in the pathogenesis of myeloid malignancies. (biomedcentral.com)
  • Although HCL is definitely a relatively rare malignancy the present data further demonstrate that mature B cell malignancies can initiate in the HSC compartment. (academicediting.org)
  • Even though stem cell source for myeloid malignancies such as myeloproliferative neoplasms myelodysplastic syndromes and acute myeloid leukemia (AML) is definitely well established a link between aberrations in HSPCs and development of mature lymphoid malignancies has been less thoroughly investigated. (academicediting.org)
  • Therefore the paradigm of linking B cell malignancies to counterparts in normal B cell development has been a predominant model to describe the cell of source for these disorders and could have got obscured the id of a far more primitive cell of origins. (academicediting.org)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • Decreased WBC count, leukopenia, is seen when supply is depleted by infection or treatment such as chemotherapy or radiation therapy, or when a hematopoietic stem cell abnormality does not allow normal growth/maturation within the bone marrow, such as myelodysplastic syndrome or leukemia. (medscape.com)
  • RUNX1 was first identified as a RUNX1-ETO fusion protein in acute myeloid leukemia (AML) and is frequently mutated in AML and myelodysplastic syndrome due to chromosomal translocation. (biolegend.com)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • 15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)
  • Some persons with clonal hematopoiesis are at increased risk for the development of myeloid cancers such as acute myeloid leukemia or myelodysplastic syndromes, a risk that increases as the hematopoietic clone expands in size.16 Stopping this expansion may delay or avert leukemic progression, and therapeutic approaches to this end are being developed and tested. (cdc.gov)
  • The expression level of its predominant encoded product, VSTM1-v1, was positively correlated with myeloid cell maturation state. (biomedcentral.com)
  • A combination of CD34/CD117/CD13/CD16 staining showed that VSTM1-v1 expression in myeloid cells was positively correlated with cell maturation state. (biomedcentral.com)
  • Monoclonal antibodies reactive with myeloid precursor cells in the bone marrow or cells undergoing early myeloid differentiation are capable of detecting antigens that may then appear at discrete stages of myeloid maturation or on cells from some patients with myeloid and/ or monocytic leukemia. (duke.edu)
  • The maturation pathway involves several stages, with differentiating cells passing through a monocyte-like stage before fully maturing into dendritic cells. (origene.com)
  • 3. Åbrink M, Grujic M, Pejler G. Serglycin is essential for maturation of mast cell secretory granule. (southernbiotech.com)
  • Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. (bdbiosciences.com)
  • Leucocyte Typing IV: White Cell Differentiation Antigens. (bdbiosciences.com)
  • Howard MR, Thomas L, Reid MM. Variable detection of myeloid antigens in childhood acute lymphoblastic leukaemia. (bdbiosciences.com)
  • Rohrschneider focused initially on cell-surface proteins in cells infected with avian oncorna viruses, identifying the envelope and group-specific antigens (gag proteins). (fredhutch.org)
  • The isolation of temperature-sensitive transformation-defective mutants of Rous sarcoma virus led to the hypothesis that the viral protein or proteins should be temperature-sensitive in its expression or function, and Dr. John Wyke and Rohrschneider found that expression of some of the cell-surface tumor antigens correlated with transformation, suggesting that they were under control of the transforming gene. (fredhutch.org)
  • Leukocyte differentiation antigens often represent important markers for the diagnosis, classification, prognosis, and therapeutic targeting of myeloid leukemia. (biomedcentral.com)
  • The development of technology to produce monoclonal antibodies for the immunological detection of cell membrane antigens has been extensively applied to the identification of human lymphocyte cell surface antigens. (duke.edu)
  • These antigens can thus be especially useful for the classification of leukemias and as a marker of myeloid cell differentiation. (duke.edu)
  • This invention is a murine monoclonal antibody used to detect cell membrane antigens specific for human myeloid cells. (duke.edu)
  • First discovered by Ralph Steinman and Zanvil A. Cohn in the 1970s, named for their tree-like branches or "dendrites", these cells patrol our bodies, ingest pathogens, process them, and present fragments (antigens) to T-cells, thus initiating a targeted immune response [2]. (origene.com)
  • cDC1: These cells are very effective at presenting antigens to CD8+ T cells, a type of cytotoxic T cell that can kill infected or cancerous cells. (origene.com)
  • cDC2: These cells are specialized in presenting antigens to CD4+ T cells, a type of helper T cell that helps coordinate the immune response. (origene.com)
  • Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
  • MNDA can be detected exclusively in nuclei of hematopoietic cells of the monocyte lineage. (guidetopharmacology.org)
  • Nevertheless, as terminally differentiated cells of the myeloid lineage, DC share with macrophages an inherent resistance to genetic modification, greatly restricting strategies available for studying their physiology and function. (ox.ac.uk)
  • Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. (biolegend.com)
  • Mice sacrificed at 12 months of age acquired no overt phenotype beyond the B lineage despite apparent activation of mitogen-activated proteins kinase (MAPK) signaling in B lineage cells (Fig. 3 A to fig and D. S3 G and F. = 0.006) upsurge in spleen weight aswell as the quantity and size of GC B cells in = 0.02) in Cd19-cre on HSC self-renewal. (academicediting.org)
  • Latest genomic analyses of leukemias of another lymphoid lineage T cell severe lymphoblastic leukemia (T-ALL) uncovered that a particular subset of T-ALL is normally highly similar on track and myeloid leukemic HSCs in gene appearance and mutational profile (25). (academicediting.org)
  • The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
  • This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
  • 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • Furthermore, the study examined the effects of cooperating oncoproteins such as Ras or Bcr/Abl, which allowed for both factor-independent proliferation and differentiation, or Bcl-2, which permitted factor-independent survival but not proliferation. (nih.gov)
  • These myeloid cell lines provide tools for examining the biochemical and genetic pathways that accompany normal differentiation as well as a system in which to dissect how other leukemic oncoproteins interfere with these pathways. (nih.gov)
  • Acute myeloid leukemia (AML) is generally regarded as a stem cell disease, and it originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity [ 1 , 2 ]. (researchsquare.com)
  • Generated to the HL60 promyelocytic cell line, it can react with all hematopoietic myeloid cells, granulocytes and normal monocytes, as well as leukemic cells from some patients with AML, AMML, and ALL. (duke.edu)
  • Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines. (lu.se)
  • A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
  • In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
  • Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
  • In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
  • STAT3 protein belongs to a group of intracellular transcription factors that mediate a variety of functions such as cellular differentiation, proliferation, and apoptosis. (biolegend.com)
  • We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
  • Etoposide does not influence phenotype of this sub-population of melanoma cells, while a combined treatment with Etoposide and Bevacizumab significantly abolished P1 sphere-forming ability, an effect associated with apoptosis of this subset of cells. (oncotarget.com)
  • Hypoxic melanoma cells sorted for VEGF-R2/CD133 positivity also undergo apoptosis when exposed to Etoposide and Bevacizumab. (oncotarget.com)
  • A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. (wikipedia.org)
  • In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons. (wikipedia.org)
  • The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
  • Hematopoietic stem cells (HSC) generate all of the blood cells throughout their life-span. (haematologica.org)
  • Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)
  • however, the level of telomerase activity is low or absent in the majority of stem cells regardless of their proliferative capacity. (nature.com)
  • Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
  • Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
  • The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
  • Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
  • In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
  • As stem cells have elongated proliferative capacity, they should have a mechanism that maintains telomere length through many cell divisions. (nature.com)
  • In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
  • MDS is a clonal disorder of myeloid stem cells. (medscape.com)
  • As an alternative, the clone may progressively deteriorate, and the appearance of marrow may return to normal as healthy stem cells repopulate it. (medscape.com)
  • Leukemia stem cells (LSCs) in acute myeloid leukemia (AML) played important roles in development of leukemia, chemotherapeutic drug resistance, and disease relapse and progression. (researchsquare.com)
  • Therefore, our results suggest that CD45 dim CD34 + CD38 - CD133 + cells in AML might have the potential of leukemia stem cells. (researchsquare.com)
  • For examples, Rhenen et al showed that a high percentage of CD34 + CD38 - stem cells at diagnosis significantly correlated with a high minimal residual disease frequency and subsequently to relapse especially after the third course of chemotherapy in AML patients. (researchsquare.com)
  • Stem cells are unique due to their ability to limitlessly self-renew and differentiate into each cell type in the adult body. (biolegend.com)
  • Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
  • RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)
  • Genetic modification of dendritic cells through the directed differentiation of embryonic stem cells. (ox.ac.uk)
  • Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)
  • Tumors contain a sub-population of self-renewing and expanding cells known as cancer stem cells (CSCs). (oncotarget.com)
  • Indeed, a 5-year survival rate of advanced stage malignant melanoma is less than 10% and resistance to chemotherapy, most likely due to the presence of cancer stem cells (CSCs), is the major cause of treatment failure [ 1 - 3 ]. (oncotarget.com)
  • Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. (southernbiotech.com)
  • Homing of lin - /CD117 + hematopoietic stem cells. (southernbiotech.com)
  • Splenectomy influences homing of transplanted stem cells in bone marrow-ablated mice. (southernbiotech.com)
  • Stem Cells Dev. (southernbiotech.com)
  • Signaling mechanism in stem cells. (iranconferences.ir)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
  • The CD13 antibody, clone L138 (also known as Leu-M7), is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c × C57BL/6 hybrid mice immunized with the KG-1a cell line. (bdbiosciences.com)
  • Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • antibody (clone AD2.35) on mouse primary astrocytes. (biolegend.com)
  • The cells were fixed, permeabilized, blocked and then stained with primary antibody, and Alexa Fluor® 594 conjugated secondary IgG (Cat. (biolegend.com)
  • This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. (abcam.com)
  • Differentiation was normal and terminal as evidenced by morphology, cell surface markers, gene expression, and functional assays. (nih.gov)
  • The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
  • RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)
  • To generate induced pluripotent stem cell clones, we reprogrammed CD34 + cells collected from a patient with a chronic myelomonocytic leukemia in which whole exome sequencing of peripheral blood monocyte DNA had identified 12 gene mutations, including a mutation in KDM6A and two heterozygous mutations in TET2 in the founding clone and a secondary KRAS (G12D) mutation. (philinbiomed.org)
  • The human eight twenty-one (ETO, MTG8 or CBFA2T1) and related myeloid transforming gene products MTGR1 and MTG16 as well as the Nervy protein contain the NHR1-4 domains. (embl-heidelberg.de)
  • In the t(8;21) translocation associated with acute myeloid leukemia (AML), the AML1(CBFA2/PEBP2alphaB) gene is juxtaposed to the MTG8(ETO/CDR) gene. (embl-heidelberg.de)
  • To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
  • Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)
  • Explore our solutions for multiple applications - vaccines, monoclonal antibodies, recombinant proteins, cell or gene therapy. (vwr.com)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
  • The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
  • To determine whether it plays a role in leukemogenesis, we characterized its expression pattern and function in bone marrow cells from AML/CML patients and myeloid leukemia cell lines. (biomedcentral.com)
  • We measured VSTM1 expression in leukemia cell lines and bone marrow biopsies from leukemia patients using qRT-PCR. (biomedcentral.com)
  • These are the malignant proliferations of abnormal clones of white blood cells within the bone marrow that are broadly categorized into lymphoid and myeloid neoplasms depending on the type of white cell proliferation. (medscape.com)
  • This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)
  • As the affected cell lines continue to divide and to provide the marrow with dysplastic cells, bone marrow dysfunction becomes apparent. (medscape.com)
  • Interestingly, the CD45 dim CD34 + CD38 - CD133 + cells were highly expressed in bone marrow of patients with AML compared to that of healthy controls (HC). (researchsquare.com)
  • In this study, we focused on measuring LSCs easily in the bone marrow cells from AML patients by developing a four-color flow cytometric analysis. (researchsquare.com)
  • Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)
  • Monocytes (MC) are bone marrow (BM) derived mononuclear phagocytes that play an important role in innate immune response and are the major immune cell population in chronic tissue inflammatory ( 1 , 2 ). (frontiersin.org)
  • C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b PE and anti-mouse Ly-6G/Ly-6C (Gr-1) (clone RB6-8C5) Spark Violet™ 423 (left) or CD11b PE only (right). (biolegend.com)
  • Dendritic cells (DCs) are bone-marrow-derived immune cells found in blood, lymphoid organs, and various tissue of the body. (origene.com)
  • Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)
  • C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse CD117-BIOT (SB Cat. (southernbiotech.com)
  • 4. Hodek M, Vávrová J, Šinkorová Z, Mokŕy J, Filip S. Hematopoietic recovery after transplantation CD117 + B220 - (LacZ + ) bone marrow cells in lethally irradiated mice. (southernbiotech.com)
  • This question is addressed in chronic myelomonocytic leukemia, a myeloid neoplasm in which clinical diversity contrasts with limited genetic heterogeneity. (philinbiomed.org)
  • Additional sex combs-like 1 ( ASXL1 ) mutations have been described in all forms of myeloid neoplasms including chronic myelomonocytic leukemia (CMML) and associated with inferior outcomes, yet the molecular pathogenesis of ASXL1 mutations ( ASXL1 -MT) remains poorly understood. (biomedcentral.com)
  • Previously, we observed that transcription factor RUNX1 mutations ( RUNX1 -MT) coexisted with ASXL1 -MT in CMML and at myeloid blast phase of chronic myeloid leukemia. (biomedcentral.com)
  • K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. (nkmaxbio.com)
  • Hairy cell leukemia (HCL) is certainly a chronic lymphoproliferative disorder seen as a somatic recently discovered somatic inhibition in hematopoiesis inside our murine choices aswell as in individuals with in HCL individuals we performed quantitative sequencing of the spot of ITD-1 p. (academicediting.org)
  • Th17 cell may have a role in severe asthma and chronic obstructive pulmonary disease (COPD). (cdc.gov)
  • The cell lines were established by conditional immortalization of primary murine marrow progenitors with an estrogen-regulated E2a/Pbx1-estrogen receptor fusion protein. (nih.gov)
  • Clones were identified that proliferated as immortalized blasts in the presence of estrogen, and that exhibited granulocytic, monocytic, or bipotential (granulocytic and monocytic) differentiation on estrogen withdrawal. (nih.gov)
  • Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. (nkmaxbio.com)
  • Thus, the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs, significantly impacting MDS-MSCs differentiation. (techscience.com)
  • The variant rs7922621 modulates AD risk through control of TSPAN14 expression in this cell type. (nature.com)
  • Restoration of VSTM1-v1 expression inhibited myeloid leukemia cells' growth. (biomedcentral.com)
  • Therefore, we speculated that VSTM1-v1 expression might be associated with the maturity of myeloid cells. (biomedcentral.com)
  • The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
  • Human MC were initially divided into three subsets based on the cell surface expression of CD14 and CD16, and recently classified based on CD40 expression ( 2 - 5 ). (frontiersin.org)
  • In humans, pDCs can be identified by the expression of several specific cell surface proteins, including CD123 (the IL-3 receptor alpha chain), BDCA-2, and BDCA-4. (origene.com)
  • The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
  • Putative CSCs were isolated from human melanoma cells of a different aggressiveness, Hs294T and A375 cell lines, grown under hypoxia using "sphere-forming assay", CD133 surface expression and migration ability. (oncotarget.com)
  • We found that a cell sub-population enriched for P1 sphere-initiating ability and CD133 expression also express larger amount of VEGF-R2. (oncotarget.com)
  • Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)
  • Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells. (lu.se)
  • Differentiation phenotypes on cells of acute myeloid leukemia studied by My7, My9, My4, Mo1 and Ja monoclonal antibodies. (bdbiosciences.com)
  • Report of the National Cancer Institute-sponsored workshop on definitions of diagnosis and response in acute myeloid leukemia. (bdbiosciences.com)
  • MDS disorders have been referred to as "preleukemias" because of their tendency to transform into acute myeloid leukemia (AML). (medscape.com)
  • This state may persist until a clone undergoes further transformation to leukemia and the marrow becomes fibrotic and aplastic. (medscape.com)
  • In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
  • These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
  • show that this approach can detect characteristic changes that affect blood cells as a result of malaria, spherocytosis, bacterial and viral infections, and leukemia. (elifesciences.org)
  • MTG8 (HGMW-approved symbol CBFA2T1) was originally identified as one of the loci involved in the t(8;21)(q22;q22) of acute myeloid leukemia. (embl-heidelberg.de)
  • Over the following week, the patient experienced fevers, and acute myeloid leukemia was diagnosed. (cdc.gov)
  • Four months after her initial acute myeloid leukemia diagnosis, the patient received a paternal haploidentical stem cell transplant. (cdc.gov)
  • The contribution of RUNX1 mutations in the pathogenesis of myeloid transformation in ASXL1 -mutated leukemia, however, remains unclear. (biomedcentral.com)
  • RUNX1-positive leukemia cells in preclinical models. (lu.se)
  • The molecular pathways of normal myeloid differentiation, as well as the mechanisms by which oncogenes disrupt this process, remain poorly understood. (nih.gov)
  • Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
  • To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
  • A critical length of telomere repeats is required to ensure proper telomere function and avoid the activation of DNA damage pathways that result in replicative senescence or cell death. (nature.com)
  • Ly6C high MC exhibited activated neutrophil degranulation, lysosome, cytokine production/receptor interaction and myeloid cell activation pathways, and Ly6C low MC presented features of lymphocyte immunity pathways in both mice. (frontiersin.org)
  • Ly6C low MC manifested activated T-cell signaling pathways and potentially can adapt the function of lymphocytes. (frontiersin.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
  • 2 Haematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. (nih.gov)
  • Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. (deepdyve.com)
  • The Organizer cordially invites abstracts and full length research papers from all over the World to participate in the World Congress on "Stem Cell Research, Cancer Biology and Applied Biotechnology" (Biotech-2014) is the premier forum for the presentation of new advances and research results in the fields of theoretical, experimental, applied molecular biology, genetics, cell sciences, cancer biology, stem cell research and applied biotechnology. (iranconferences.ir)
  • Avantor® can help equip your life sciences lab with the products, equipment, and supplies you need - whether you work in cell biology, genomics, proteomics, or other fields. (vwr.com)
  • Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
  • A major limitation in approaching this problem has been the lack of suitable cell lines that exhibit normal, terminal, and synchronous differentiation in the absence of endogenous oncoproteins and in response to physiologic cytokines, and whose differentiation can be arrested by ectopically expressed human oncoproteins. (nih.gov)
  • During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
  • T helper (Th) 2 cells, which recruit and accumulate in the lungs and produce a range of different effector cytokines. (cdc.gov)
  • However, more recent studies have revealed the potential collaboration of other helper T cells and their cytokines in this process. (cdc.gov)
  • The aim of this review is to summarize the current knowledge about the possible roles of newly identified helper T cells derived cytokines (IL-9, 17, 22, 25 and IL-33) in asthma. (cdc.gov)
  • For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
  • However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
  • In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
  • Embryonic stem (ES) cells are, by contrast, relatively amenable to genetic modification. (ox.ac.uk)
  • We captured a part of the genetic heterogeneity observed in the patient, i.e. we analyzed five clones with two genetic backgrounds, without and with the KRAS (G12D) mutation. (philinbiomed.org)
  • These analyses also disclosed significant discrepancies in the behavior of hematopoietic cells derived from induced pluripotent stem cell clones with similar genetic background, correlating with limited epigenetic changes. (philinbiomed.org)
  • CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. (thermofisher.com)
  • This report describes clonal, granulocyte-macrophage colony-stimulating factor-dependent myeloid cell lines that exhibit these properties. (nih.gov)
  • Likewise Kikushige lately demonstrated which the propensity to create clonal B cells in sufferers with the older B cell malignancy CLL is normally obtained in the HSC area (24). (academicediting.org)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
  • In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
  • Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
  • Moreover our use of granulocyte DNA as matched somatic cells may have obscured additional mutations acquired early in the hematopoietic compartment and present at related frequencies in granulocyte and HCL DNA. (academicediting.org)
  • Interleukin-21 (IL-21) is produced by activated T cells and it plays many diverse roles by regulating the functions of normal and abnormal cells. (intechopen.com)
  • Screening for recurrently mutated genes in the mononuclear cell fraction revealed mutations in SF3B1 in 39 of 40 cases (97.5%), combined with TET2 and DNMT3A in 11 (28%) and 6 (15%) patients, respectively. (nih.gov)
  • The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
  • Here, we describe how another defining property of ES cells, their demonstrable pluripotency, may be harnessed for their directed differentiation along the DC pathway, enabling the generation of limitless numbers of DC faithfully expressing candidate genes of interest. (ox.ac.uk)
  • explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
  • The concept of polymorphonuclear neutrophils' (PMNs') single-track fate as terminally differentiated and short-lived primary effector cells of host defense and acute inflammation is rapidly evolving. (aai.org)
  • Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)
  • In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. (elifesciences.org)
  • Polymorphonuclear neutrophils (PMN) have distinct phenotypes that drive inflammation but also regulate lymphocytes and are the rate-limiting cell for generating anti-inflammatory lipoxin A 4 (LXA 4 ). (aai.org)
  • Examples of the former are mast cell deficiency and severe macrocytic anemia while the latter mutation has been identified in mastocytomas. (southernbiotech.com)
  • at 16 weeks after transplantation) competitive advantage of mutation affects the differentiation and function of different committed hematopoietic progenitors which may drive the disease phenotype. (academicediting.org)
  • Second although our analyses of the VAF of the mutation in HSPC subsets from HCL individuals these analyses used cDNA where the level of wild-type and mutant manifestation may differ from your VAF at the level of genomic DNA in these cell subsets. (academicediting.org)
  • POT1 mutation carriers had a range of benign and malignant neoplasms involving epithelial, mesenchymal, and neuronal tissues in addition to B- and T-cell lymphoma and myeloid cancers. (cdc.gov)
  • Rising evidence shows that HSPCs might enjoy essential roles in various other neoplasms of mature B cells. (academicediting.org)
  • Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
  • Interleukin-21 (IL-21) was first identified in 2000 through screening of a cDNA library from CD3+ human T cells [ 1 ]. (intechopen.com)
  • Mouse anti-human CD45-FITC (Clone 2D1, Cat No. 347463), Mouse Anti-human CD34-PE [Clone 8G12 (also known as HPCA2), Cat No. 348057], Mouse anti-human CD38-PE-Cy™5 (Clone HIT2, Cat No. 555461) and appropriated isotype control antibodies were purchased from BD Biosciences (San Diego, CA, USA). (researchsquare.com)
  • Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714-82) (blue histogram) or Anti-Human CD14 Super Bright 645 (purple histogram). (thermofisher.com)
  • Additionally, the high levels of the CD45 dim CD34 + CD38 - CD133 + cells in AML patients were an independently significant poor risk factor for overall survival and event free survivals. (researchsquare.com)
  • CD34 + cells from an age-matched healthy donor were also reprogrammed. (philinbiomed.org)
  • Molecular cloning of cDNA indicated that the AML1-MTG8-binding protein (MTGR1) is highly related to MTG8 and similar to Drosophila Nervy. (embl-heidelberg.de)
  • In the context of cancer, appropriately activated DCs can induce anti-tumor immunity by activating innate immune cells and tumor-specific lymphocytes that target cancer cells. (mdpi.com)
  • Around this time, Dr. Chuck Sherr at St. Jude's and Dr. Richard Stanley at Albert Einstein identified the normal cellular Fms protein as the cell surface receptor for a growth factor known as the macrophage colony-stimulating factor or M-CSF. (fredhutch.org)
  • Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
  • In normal cells, glucose participates in cellular energy production through glycolysis as well as through its complete catabolism via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation (OXPHOS). (hindawi.com)
  • Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
  • This was one of the initial discoveries that mutations in a cell surface receptor could initiate oncogenesis, just one year after the avian erythroblastosis virus oncogene was found to be a mutant EGF receptor. (fredhutch.org)
  • The differentiation of the cells could be arrested by heterologous oncoproteins including AML1/ETO, PML/RARalpha, PLZF/RARalpha, Nup98/HoxA9, and other Hox proteins. (nih.gov)
  • RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
  • Depressed lymph node PMN and LXA 4 in females correlated with an increase in effector T cells (Th1 and Th17), a decrease in regulatory T cells (Treg), and increased dry eye pathogenesis. (aai.org)
  • Ab depletion of tissue PMN abrogated LXA 4 formation in lymph nodes, as well as caused a marked increase in Th1 and Th17 cells and a decrease in Tregs. (aai.org)
  • Those first immunoprecipitation experiments also revealed a related protein in uninfected cells, one of the first sightings of the Src proto-oncogene product. (fredhutch.org)
  • Rb , or retinoblastoma protein, is a key regulator of the cell cycle, particularly during the transition from the G1 to S phases. (biolegend.com)
  • Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)
  • In mice, they express the cell surface protein XCR1, while in humans, they can be identified by the presence of CD141 (or BDCA-3). (origene.com)
  • The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis. (techscience.com)
  • Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)
  • Recent years have witnessed a progressive acceptance of the dual role played by dendritic cells (DC) in the initiation of immune responses and their specific attenuation through the induction of immunological tolerance. (ox.ac.uk)
  • Unlike other immune cells, dendritic cells are not just a single entity, but a complex assortment of subtypes with varying functions. (origene.com)
  • Several models are proposed to describe the development and differentiation of dendritic cells (DCs). (origene.com)
  • In humans and mice, conventional dendritic cells are divided into two main subtypes: cDC1 and cDC2, each with distinct functions and markers [2]. (origene.com)
  • Plasmacytoid dendritic cells (pDCs), are round plasma-shaped cells specialized for the production of large amounts of type I and type III interferon in response to viral infection. (origene.com)
  • Aberration occurs in a stem cell that can give rise to multiple lineages. (medscape.com)
  • Hierarchical differentiation competence in response to retinoic acid ensures stem cell maintenance during mouse spermatogenesis. (southernbiotech.com)
  • Furthermore, their propensity for self-renewal, one of the cardinal features of a stem cell, permits cloning at the single cell level and the rational design of ES cell lines, uniformly expressing a desired, mutant phenotype. (ox.ac.uk)