Mycoplasma fermentans has been associated with rheumatoid arthritis. (biomedcentral.com)
reported the presence of Mycoplasma fermentans in the synovial fluid of patients with rheumatoid arthritis [ 7 ], but these results could not be reproduced in further investigations probably because of the fastidious growth requirements of the microorganism [ 9 ]. (biomedcentral.com)
This mycoplasma has been detected in blood of patients with rheumatoid arthritis, producing systemic mycoplasmal infections [ 14 ], these results suggest that M. fermentans may play a role in the development of the disease. (biomedcentral.com)
Site-specific proteolysis of the MALP-404 lipoprotein determines the release of a soluble selective lipoprotein-associated motif-containing fragment and alteration of the surface phenotype of Mycoplasma fermentans . (zju.edu.cn)
A Mycoplasma fermentans -derived synthetic lipopeptide induces AP-1 and NF-κB activity and cytokine secretion in macrophages via the activation of mitogen-activated protein kinase pathways. (zju.edu.cn)
Induction of leukemia cell differentiation and apoptosis by recombinant P48, a modulin derived from Mycoplasma fermentans . (zju.edu.cn)
Some mycoplasmas, such as M. penetrans, M. fermentans and M. pirum, can enter a variety of tissues and cells and cause a broad spectrum of signs and symptoms [3]. (thearthritiscenter.com)
Mitogen3
M. arthritidis mitogen (MAM), a superantigen produced by M. arthritidis , activates T cells by forming a complex between the major histocompatability complex II on antigen presenting cells and the T cell receptor on CD4+ T lymphocytes. (biomedcentral.com)
Much of the disease phenotype and outcomes are influenced by the M. arthritidis mitogen (MAM) superantigen produced by the organism. (biomedcentral.com)
It was equivalent to the experiences, which proposed that the conversation of M. arthritidis mitogen with TLR2 and TLR4 may well participate in an significant function in ailment outcomes by M. arthritidis [29]. (ack1inhibitor.com)
Strains2
Mycoplasma arthritidis causes arthritis in specific mouse strains. (biomedcentral.com)
The largest group of the class Mollicutes is divided into more than 100 mycoplasma species, which are further subclassified into various strains. (thearthritiscenter.com)
Reagents2
Your culture is easily treatable with InvivoGen's anti-mycoplasma reagents. (invivogen.com)
Mycoplasma is a major problem in cell culture, with various sources of contamination such as lab operators, dirty water baths/incubators, and biological reagents. (invivogen.com)
Hominis1
Bartholomew isolated M. hominis , M. hyorhinis and M. arthritidis from the synovial fluid in 14 of 17 patients with rheumatoid arthritis, systemic lupus erythematosus and Reiter's Syndrome in 1965 [ 8 ]. (biomedcentral.com)
Bacteria3
Mycoplasmas are the smallest self-replicating, pleotrophic bacteria that lack cell walls [1,2]. (thearthritiscenter.com)
A species of gram-negative bacteria causing PNEUMONIA, MYCOPLASMA in calves. (uchicago.edu)
Mycoplasma dispar also possibly plays a synergistic role with other BACTERIA and VIRUSES to cause other RESPIRATORY TRACT DISEASES in calves. (uchicago.edu)
TLR22
In this study, we evaluated serum hepcidin, transferrin saturation, ferritin, IL-6, IL-1, and hemoglobin levels in M. arthritidis injected C3H/HeJ (TLR2 +/+ , TLR4 -/- ) mice and C3H/HeSnJ (TLR2 +/+ , TLR4 +/+ ) mice over a 21 day period. (biomedcentral.com)
To determine if hepcidin could be expressed independent of TLR4 activation, we measured serum hepcidin levels in C3H/HeJ mice (TLR2 +/+ , TLR4 -/- ) and compared the values to C3H/HeSnJ mice (TLR2 +/+ , TLR4 +/+ ) after infection with M. arthritidis . (biomedcentral.com)
Proteins1
Interactions between mycoplasma lipid-associated membrane proteins and the host cells[J]. Journal of Zhejiang University Science B, 2006, 7(5): 342-350. (zju.edu.cn)
Commonly4
Simply prepare your sample and add our proprietary Reaction Mix to target and amplify the 16S rRNA gene for the most commonly found mycoplasma species in cell culture. (invivogen.com)
MycoStrip™ has been specifically designed to detect the Mycoplasma and Acholeplasma species that most commonly contaminate cell culture. (invivogen.com)
These findings may help explain why mycoplasmas are commonly isolated from the joints of rheumatic patients. (biomedcentral.com)
Mycoplasmas are commonly found in the oral cavity and as symbiotic gut flora. (thearthritiscenter.com)
Mycoplasmas are often found as extracellular parasites attached to the external surfaces of host cells, but some species invade host tissues and cells, and replicate intracellularly. (thearthritiscenter.com)
Respiratory3
The primary habitats of human and animal mycoplasmas are the mucous surface of the respiratory and urogenital tracts, the eyes, mammary glands and joints [ 5 ]. (biomedcentral.com)
Mycoplasmas are common inhabitants of the human respiratory and genitourinary tracts where they produce chronic infections, they also are able to invade other tissues and in some cases induce an autoimmune response [ 5 ]. (biomedcentral.com)
And Mycoplasma infection correlates with the an infection of other secondary respiratory pathogens by inducing the immunomodulation of host animals [eighteen?1]. (ack1inhibitor.com)
Arthritis3
Mycoplasma arthritidis ( M. arthritidis ) is a rodent pathogen that causes arthritis and a toxic shock-like syndrome in C3H mice. (biomedcentral.com)
M. arthritidis injected mice have been used as a mouse model of human inflammatory arthritis for more than 30 years. (biomedcentral.com)
These abilities are used by mycoplasmas during the induction of natural arthritis in animals. (biomedcentral.com)
Cells2
Interplay between mycoplasmas and host target cells. (zju.edu.cn)
Some mycoplasmas can enter a variety of tissues and cells and cause system-wide or systemic signs and symptoms. (thearthritiscenter.com)
Experimental1
MycoStrip™ is able to detect as low as 10-10 2 CFU/ml and thus, can be used to detect mycoplasma contamination before it significantly affects experimental results, which typically occurs at ~10 7 CFU/ml [1]. (invivogen.com)
Special1
Easy to perform, and unlike most other mycoplasma detection assays, no special lab equipment is required. (invivogen.com)
Year1
This graph shows the total number of publications written about "Mycoplasma dispar" by people in this website by year, and whether "Mycoplasma dispar" was a major or minor topic of these publications. (uchicago.edu)
With over 40 years of experience in developing mycoplasma solutions for the scientific community, InvivoGen's MycoStrip™ offers a new method for the detection of mycoplasma in cell culture, allowing you to combat the contamination. (invivogen.com)
Detection of cell culture contaminating mycoplasma by MycoStrip™ is based on isothermal PCR. (invivogen.com)
Besides affecting cell growth and morphology, mycoplasmas are able to alter metabolic, immunological and biochemical functions [3]. (thearthritiscenter.com)
Complex1
Mycoplasmas have also been shown to have a complex relationship with the immune system [4]. (thearthritiscenter.com)
Species2
So far culture is most commonly used for detection of genital Mycoplasma, but it requires special handling, complex media, and cultivation positive samples need further testing to determinate the species cultivated [ 16 ]. (biomedcentral.com)
Polyclonal sera have been and continue to be helpful reagents for identifying mycoplasmas to the species level but are not as discriminating as MAbs for studying differences among strains, especially with respect to defining pathogenic properties of an isolate. (docksci.com)
Pulmonis1
Mycoplasma pulmonis is deemed the most important in the rat and Sendai virus the most important in the mouse. (nih.gov)
Amplicon1
The detection of the mycoplasma-specific amplicon is based on using the specific FAM-labelled probe. (eurx.com.pl)
Reagents1
Duplex Mycoplasma qPCR Kit contains reagents and primers for the amplification ofthe 16S rRNA coding region in the mycoplasma genome. (eurx.com.pl)
Detection3
Duplex Mycoplasma qPCR Kit is a ready-to-use system for thequantitative detection of Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma, Ureaplasma) DNA in cell cultures, animal tissue samples and other biological matrices. (eurx.com.pl)
Duplex Mycoplasma qPCR Kit provides the efficient detection of Mollicutes with high sensitivity (LOD ≤ 10 CFU/ml or LOD ≤ 5 Mollicutes DNA copies per reaction). (eurx.com.pl)
When performing a PCR detection of mycoplasmas in a GMP production surrounding, this very crude and basic protocol has to be changed in several aspects and controls have to be included. (incelligence.de)
Contamination1
Now you grow and passage the cells (best without antibiotics) for at least a week to give the potential mycoplasma contamination time to grow. (incelligence.de)
Polymerase1
Duplex Mycoplasma Master Mix contains onTaq DNA Polymerase, optimized reaction buffer and dNTPs. (eurx.com.pl)
Specific1
The amplification of the inner control does not interfere with the amplification of the specific mycoplasma DNA and confirms DNA isolation and/or PCR reaction correctness. (eurx.com.pl)