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Mycobacterium avium subsp. paratuberculosisParatuberculosisMycobacterium aviumMycobacterium avium ComplexCattle DiseasesMycobacteriumMycobacterium avium-intracellulare InfectionCattleMycobacterium tuberculosisMycobacterium InfectionsDNA, BacterialMilkMycobacterium bovisSheep DiseasesTuberculosis, AvianFecesPolymerase Chain ReactionMycobacterium smegmatisTuberculosisNontuberculous MycobacteriaDairyingBisonDNA Transposable ElementsAntibodies, BacterialBacteriological TechniquesAntigens, BacterialGoatsColony Count, MicrobialGoat DiseasesMycobacteriophagesBacterial ProteinsBenzophenoneidumSensitivity and SpecificityMycobacterium Infections, NontuberculousVeterinary MedicinePolymorphism, Restriction Fragment LengthDeerCulture MediaCrohn DiseaseEthambutolBacterial Typing TechniquesEnzyme-Linked Immunosorbent AssayMycobacterium lepraeMycobacterium kansasiiSheepRuminantsCetylpyridiniumRifabutinBacterial LoadMolecular Sequence DataBacterial SheddingAnimals, DomesticMacrophagesIleumSpecies SpecificityMycobacterium phleiAnimals, WildMycobacterium fortuitumCheeseMycobacterium scrofulaceumInterferon-gammaAntitubercular AgentsManureNative Polyacrylamide Gel ElectrophoresisMicrobial ViabilitySequence Analysis, DNAMycobacterium chelonaeClarithromycinMycobacterium marinumGenome, BacterialImmunomagnetic SeparationGenes, BacterialTuberculosis, BovineMolecular TypingAIDS-Related Opportunistic InfectionsBacterial VaccinesClofazimineBase SequenceGranulomaChaperoninsTuberculosis, PulmonaryVirulenceMycobacterium ulceransMycolic AcidsMicrobial Sensitivity TestsRifamycinsMacrophage ActivationGlycolipidsAnti-Bacterial AgentsLeukocytes, MononuclearChaperonin 60RifampinSoil MicrobiologyDNA Primers
Johne'sSubspeciesInfectionPathogenCausativeAntibodiesGenomesAntigensAbstractDetectionIsolatesTuberculosisAssayGenomeMycobactin-dependent mycobacteriaPurified protein deBovisNontuberculous mycobacteriaStrainsPorcineGenesPathogenicDiagnosisRuminantsIsolationParatuberkuloseStreptococcusSubclinicalOpportunisticMilkHumansStrainMycobacterialCattleFecesSpeciesSignificant economic lossesCultureCountsDeerSamplesPresenceRiskFieldPositiveComplex
Johne's9
Subspecies4
  • Mycobacterium avium subspecies paratuberculosis (MAP) is an obligate pathogenic bacterium in the genus Mycobacterium. (wikipedia.org)
  • One study has argued that the presence of antibodies against Mycobacterium avium subspecies paratuberculosis is associated with increased propensity of patients with Crohn's disease to receive biological therapy. (wikipedia.org)
  • Subspecies determination by rpoB sequencing and IS 1245 /IS 901 analysis showed that 98.9% of Belgian human M. avium strains belong to the subspecies hominissuis (MAH). (eurosurveillance.org)
  • Mycobacterium avium subspecies paratuberculosis (Map) stellt demnach nicht nur eine Gefahr für Rinder und andere Wiederkäuer dar, sondern kann als Zoonoseerreger nicht sicher ausgeschlossen werden. (fu-berlin.de)
Infection3
Pathogen2
  • paratuberculosis (MAP) is an obligate intracellular pathogen that infects many ruminant species. (psu.edu)
  • paratuberculosis (MAP) is an intracellular pathogen causative of Jonhe disease or paratuberculosis in most domestic or wild rumiant species. (cibnor.mx)
Causative1
  • paratuberculosis (MAP), the causative agent of Johne disease in ruminants, has been incriminated as the cause of Crohn disease in humans. (cdc.gov)
Antibodies1
  • paratuberculosis or M. bovis BCG Pasteur did not produce cross-reactive antibodies that could be detected by the DPP VetTB assay. (nih.gov)
Genomes3
  • Ninety-six primer sets were used for amplified fragment length polymorphism (AFLP) to characterize the genomes of 20 Mycobacterium avium subsp. (tamu.edu)
  • This work revealed the presence of extensive polymorphisms in the genomes of M. avium subsp. (tamu.edu)
  • Representative genomes are assigned to type strain assemblies if there is no current reference genome or if one other than the reference is scientifically significant and exhibits strong sequence diversity as compared to the assigned reference genome(s) (ex: Mycobacterium avium subsp. (nih.gov)
Antigens2
  • paratuberculosis specific antigens in DNA vaccinated and Map infected mice. (sciensano.be)
  • paratuberculosis (PPD)--and antigens: tetanus toxoid and KLH. (nih.gov)
Abstract1
Detection7
Isolates6
  • paratuberculosis isolates. (tamu.edu)
  • paratuberculosis field isolates, 1 American Type Culture Collection (ATCC) M. avium subsp. (tamu.edu)
  • avium isolates (ATCC 35716 and Mac 104). (tamu.edu)
  • paratuberculosis isolates that may be used to establish diagnostic patterns useful for the epidemiological tracking of M. avium subsp. (tamu.edu)
  • avium isolates failed to act as templates for PCR amplification in seven of eight reactions. (tamu.edu)
  • Virulence and immunogenicity of genetically defined human and porcine isolates of M. avium subsp. (sciensano.be)
Tuberculosis2
Assay4
  • The FASTPlaqueTB phage assay provided more rapid enumeration of surviving M. avium subsp. (calfstart.com)
  • paratuberculosis derived using the phage assay and culture results were not significantly different (P 0.077). (calfstart.com)
  • Objective -To determine whether cattle testing positive for Mycobacterium avium subsp paratuberculosisas determined by microbial culture of feces or antibody ELISA were more likely to have false-positive responses on the caudal fold tuberculin (CFT) test or interferon-γ (IFN-γ) assay for Mycobacterium bovis than cattle testing negative for M paratuberculosis . (avma.org)
  • Conclusions and Clinical Relevance -No significant association was detected among cattle testing positive for M paratuberculosis as determined by microbial culture of feces and antibody ELISA and positive CFT test and IFN-γ assay results for M bovis . (avma.org)
Genome3
  • avium genome. (tamu.edu)
  • Genome-wide mosaicism within Mycobacterium abscessus: evolutionary and epidemiological implications. (cdc.gov)
  • paratuberculosis (MAP) and Crohn's disease, and a progress report on the Map genome project at the National Animal Disease Laboratory. (vin.com)
Mycobactin-dependent mycobacteria1
Purified protein de2
  • Plasma samples were tested for antibody against M paratuberculosis , and IFN-γ after stimulation with purified protein derivative tuberculin from M bovis or M avium . (avma.org)
  • At recapture, blood was sampled for determination of plasma levels of PCBs and organochlorine pesticides (OCPs) and lymphocyte proliferation after in vitro stimulation with specific mitogens--phytohemagglutinin (PHA), pokeweed mitogen (PWM), concanavalin A (Con A), lipopolysaccharide (LPS), and purified protein derivative of Mycobacterium avium subsp. (nih.gov)
Bovis3
  • We evaluated the diagnostic accuracy of this test by using serum samples collected from groups of white-tailed deer experimentally inoculated with Mycobacterium bovis, M. avium subsp. (nih.gov)
  • paratuberculosis, or M. bovis BCG Pasteur. (nih.gov)
  • PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. (visavet.es)
Nontuberculous mycobacteria2
  • Pulmonary disease due to nontuberculous mycobacteria: an epidemiologist's view. (eurosurveillance.org)
  • Nontuberculous mycobacteria: opportunistic environmental pathogens for predisposed hosts. (eurosurveillance.org)
Strains2
  • Ninety-two human M. avium strains were selected for a retrospective study. (eurosurveillance.org)
  • paratuberculosis strains in 319 tissue samples from cattle herds and goat flocks. (visavet.es)
Porcine1
Genes4
  • However, because this gene is similar to genes in other mycobacteria, there is a need for sensitive and reliable methods to confirm the presence of MAP. (biomedcentral.com)
  • The acquisition of foreign genes via horizontal gene transfer has been postulated to contribute to its pathogenesis, as these genetic elements are absent from its putative ancestor, M. avium subsp. (psu.edu)
  • En base a los resultados de predicción inmunogénica, se seleccionaron los genes que codifican para las proteínas MAP0164, MAP0189 y MAP2100. (cibnor.mx)
  • Luego, los genes MAP2100 y MAP0189 se clonaron en el vector p320 para la modificación genética del cloroplasto de C. reinhardtii. (cibnor.mx)
Pathogenic2
  • This report suggests a pathogenic role of MAP for immunocompromised patients, raising the question of whether this strain so far has not been detected because of its limited growth, whether it has been misidentified as M. avium, or whether its occurrence in infections is low. (medscape.com)
  • Revisiting the expression signature of pks15/1 unveils regulatory patterns controlling phenolphtiocerol and phenolglycolipid production in pathogenic mycobacteria. (ulisboa.pt)
Diagnosis2
Ruminants1
  • Paratuberculosis (MAP) in fecal and tissue samples (e.g., small intestine, mesenterial lymph nodes) from ruminants and culture slants (individual and pooled samples). (indical.com)
Isolation1
Paratuberkulose1
Streptococcus2
  • Investigation of falsely reported resistance of Streptococcus equi subsp. (wanfangdata.com.cn)
  • paratuberculosis K-10 or Streptococcus thermophilus JIM 8232). (nih.gov)
Subclinical1
  • Since paratuberculosis is often subclinical, under-reporting is suspected, even though the disease is notifiable in numerous countries. (nih.gov)
Opportunistic1
  • Opportunistic infections caused by various Mycobacterium species are among the leading AIDS indicator diseases in HIV-positive patients (1) . (cdc.gov)
Milk5
  • paratuberculosis in Middlebrook 7H9 broth and whole and semiskim milk was investigated using a laboratory-scale UV machine that incorporated static mixers within UV-penetrable pipes. (calfstart.com)
  • paratuberculosis suspended in milk (0.5- to 1.0-log10 reduction per 1,000 mJ/ml) than that suspended in Middlebrook 7H9 broth (2.5- to 3.3-log10 reduction per 1,000 mJ/ml). (calfstart.com)
  • View of Is the effectivity of copper ions treatment of milk enough to block Mycobacterium avium subsp. (ajvs.cl)
  • They found that raw milk is a significant source of exposure Mycobacterium avium subsp. (thedairysite.com)
  • paratuberculosis (MAP), causes weight loss, diarrhoea, and reduced milk yields in clinically infected cattle. (aber.ac.uk)
Humans1
Strain1
Mycobacterial3
  • We describe the case of an HIV-infected patient who had a severe mycobacterial disorder thought to be caused by M. avium complex. (medscape.com)
  • In the case we describe, mycobacterial growth could be detected in liquid media in 8 of 21 specimens, all confirmed as M. avium complex/ M. paratuberculosis . (medscape.com)
  • Mycobacterium avium complex is the most important mycobacterial species. (cdc.gov)
Cattle2
  • Forty-five (4.3%) and 115 (11.0%) cattle tested positive for M paratuberculosis as determined by microbial culture of feces and antibody ELISA, respectively. (avma.org)
  • Cattle with positive responses for M paratuberculosis appeared to have an increased likelihood of false-positive results on the CFT test, although this association was not significant. (avma.org)
Feces2
  • Microbiologic analyses included culture for mycobacteria (liquid media: BACTEC 460TB or MGIT [Becton, Dickinson and Company, Cockeysville, MD] and solid media produced in-house, all media without supplementation of mycobactin) from at least 21 specimens (blood, urine, sputum, biopsy, feces) over a 3-year period. (cdc.gov)
  • Of these, eight specimens (blood, feces, and biopsy) were positive for mycobacteria in liquid media after 6 to 16 weeks of incubation. (cdc.gov)
Species3
  • M. avium complex includes the species M. avium and M. intracellulare , with M. avium consisting of M. avium subsp. (cdc.gov)
  • For species identification, AccuProbe assays (Gen-Probe, San Diego, CA) for M. avium complex were performed on liquid media, all yielding strong positive results. (cdc.gov)
  • The resulting sequence was compared with those stored in the International Nucleotide Sequence Database (8) , showing the signature sequence of M. avium / M. paratuberculosis , which is identical for both species and confirmed the AccuProbe results. (cdc.gov)
Significant economic losses1
  • Paratuberculosis in domestic livestock entails significant economic losses due to several factors (e.g. reduced production, premature culling and increased veterinary costs). (nih.gov)
Culture3
Counts1
  • Despite the fact that plaque counts were consistently 1 to 2 log10 lower than colony counts throughout the study, UV inactivation rates for M. avium subsp. (calfstart.com)
Deer1
  • Deer experimentally inoculated with either M. avium subsp. (nih.gov)
Samples1
  • Herds were selected based on the presence of risk factors for paratuberculosis (74 herds), sampling was performed by veterinarians who collected negative samples in all of their herds in a previous survey (75 herds) and clinical paratuberculosis cases (nine herds). (vetline.de)
Presence1
Risk2
Field1
Positive1
Complex1
  • Since M. avium complex usually grows very well, the primary identification was questionable. (cdc.gov)