Mutation nuclear localization sequence nuclear localization signals. Medical search. Frequent questions

However, in an investigation of BRAF localisation using fluorescence microscopy combined with subcellular fractionation of Green Fluorescent Protein (GFP)-tagged proteins expressed in NIH3T3 cells, surprisingly, we detected N-terminally truncated BRAF (ΔBRAF) in both nuclear and cytoplasmic compartments. (bath.ac.uk)
SpCas9 was also tagged with enhanced yellow fluorescent protein (EYFP) gene for visualization of the SpCas9 expression signal in subcellular sites of Eimeria . (biomedcentral.com)
Nucleophosmin (NPM) is known to regulate ARF subcellular localization and MDM2 activity in response to oncogenic stress, though the precise mechanism has remained elusive. (oncotarget.com)
The subcellular localization of OsGAI in vivo shows that OsGAI-GFP fusion protein locates in the nucleus concerned with a nuclear localization signal (NLS) [2] . (ic4r.org)
Accordingly, the subcellular localization of NLS1 and NLS2 in TWIST protein was investigated in U2-OS cells following co-transfection with E12. (uni-marburg.de)
To assess the role of NES2 in regulating ESE-1 subcellular localization and subsequent transformation potency, we site-specifically mutagenized NES2, within full-length GFP-ESE-1 and GFP-NES2-SAR reporter constructs. (biomedcentral.com)
These data highlight that ESE-1 contains NLS and NES signals that play a critical role in regulating its subcellular localization and function, and that an intact SAR domain mediates MEC transformation exclusively in the cytoplasm, via a novel nontranscriptional mechanism, whereby the SAR motif is accessible for ligand and/or protein interactions. (biomedcentral.com)

At the cellular level, the actions of glucocorticoids are mediated by a 94-kd protein, the glucocorticoid receptor (GR). The human (h) GR belongs to the steroid/thyroid/retinoic acid superfamily of nuclear receptors and functions as a ligand-dependent transcription factor that regulates the expression of glucocorticoid-responsive genes positively or negatively. (medscape.com)
The DNA-binding domain (DBD) of the hGRα corresponds to amino acids 420-480 and contains 2 zinc finger motifs through which the hGRα binds to specific DNA sequences, the glucocorticoid-response elements (GREs) in the promoter region(s) of target genes. (medscape.com)
INTRODUCTION: Interpretation of results from mutation screening of tumour suppressor genes known to harbour high risk susceptibility mutations, such as APC, BRCA1 , BRCA2 , MLH1 , MSH2 , TP53 , and PTEN , is becoming an increasingly important part of clinical practice. (xenbase.org)
Subsequent repair of the DSBs is carried out by the host cell through either non-homologous end-joining pathway (NHEJ), leading to insertion and deletion mutations in the targeted genes, or homologous direct repair (HDR) in the presence of a DNA donor template. (biomedcentral.com)
Specific mutations on those points molecular evolution of specific genes of influenza viruses, may enable an avian influenza virus to become a human comprehensive comparisons among the nucleotide virus. (cdc.gov)
PB2 genes (viral ribonucleoproteins [RNPs]) and are most- encoded protein sequences have not been extensively ly located in the functional domains related to RNP-RNP reported. (cdc.gov)
The multifunctional regulator nuclear factor erythroid 2-related factor (Nrf2) is considered not only as a cytoprotective factor regulating the expression of genes coding for anti-oxidant, anti-inflammatory and detoxifying proteins, but it is also a powerful modulator of species longevity. (springer.com)
The discovery of the antioxidant response element (ARE) have led to the conclusion that the battery of genes, including glutamate-cysteine ligase (GCL), thioredoxin reductase 1 (Txnrd1), NAD(P)H-quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HMOX1) is regulated through Nrf2 binding to this consensus binding sequence [ 3 ]. (springer.com)
Nrf2 stabilization and increase in its half-life even to 200 min [ 9 ] allows nuclear translocation and activation of transcription of cytoprotective genes (Fig. 1 ). (springer.com)
Up coming, we ranked molecular apocrine genes primarily based on their fold modify in expres sion following the modulation of AR ERK signaling. (cdantigens.com)
These information indicate that AR ERK signal ing regulates the transcription of selective molecular apocrine genes. (cdantigens.com)
There is no longer a need for signals to activate these genes. (cancerquest.org)
Many of these genes are responsible for providing the positive signals that lead to cell division. (cancerquest.org)
ALL cancers have lots of additional changes, the so-called 'passenger' mutations, that may contribute to the cancer, but are not the main genes. (cancerquest.org)
Although pheochromocytoma has classically been associated with 3 syndromes-von Hippel-Lindau (VHL) syndrome, multiple endocrine neoplasia type 2 (MEN 2), and neurofibromatosis type 1 (NF1)-there are now 10 genes that have been identified as sites of mutations leading to pheochromocytoma. (medscape.com)

We demonstrate that AKT and PI3K inhibitors may be effective in treatment of therapeutically resistant tumors with elevated AKT and carrying gain of function mutations in p53. (oncotarget.com)
Three semi-dominant dwarf mutants (Slr1-d1, Slr1-d2 and Slr1-d3) associated with this gene have been identified, which were caused by gain-of-function mutations in the N-terminal region of SLR1 [4] . (ic4r.org)

3 WT1 is also overexpressed and mutated in AML, in which it confers inferior survival, 4 , 5 yet the interplay between these two signaling proteins has not been examined previously within a hematopoietic context. (haematologica.org)
If the nuclear exclusion of truncated BRCA2 proteins is also observed in primary tumour samples, then individuals with truncating mutations in BRCA2 could be screened by immunostaining. (biomedcentral.com)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. (nih.gov)
The BLM protein shows similarity to sequences of RecQ subfamily of ATP-dependent DNA helicases, including RecQ, Sgs1, Rqh1, and the human RECQL and WRN proteins. (lu.se)
SUMOylation is a post-translational modification of proteins that has been found to play a major role in the Wnt/β-catenin signaling pathway. (frontiersin.org)
Cell membrane proteins can trigger intracellular signal transduction and regulate cell proliferation, differentiation and survival. (cgu.edu.tw)
Nuclear localization sequences and conserved domains of the MoSom1 and MoCdtf1 proteins are crucial for 192203-60-4 his or her biological function. (bio2009.org)
Rgs1, a regulator of G-protein signaling, interacts with all the three G subunits and functions as a negative regulator of G-proteins in [52]. (bio2009.org)

A causal role for TDP-43 assembly in neurodegeneration is evidenced by dominantly inherited missense mutations in TARDBP, the gene encoding TDP-43, that promote assembly and give rise to ALS and FTLD3,4,5,6,7. (lafurnitura.com)
Understanding missense mutations in the BRCA1 gene: an evolutionary approach. (xenbase.org)

The loss of proper response to DNA damage leads to genomic instability such as gene mutations, incomplete replication and the loss or gain of chromosomes to future generations. (biomedcentral.com)

A series of plasmids encoding GFP-tagged, C-terminal fragments of BRCA2 containing mutations in the different putative NLSs were used for further immunofluorescence studies. (biomedcentral.com)
FAK also includes putative nuclear localization sequences (NLS) inside the F2 lobe of its FERM site and may localize towards the nucleus upon receipt of mobile tension, where it binds to p53 (Lim et?al. (ufe-eg.org)
To map the minimal functional nuclear localization (NLS) and nuclear export (NES) signals, we fused in-frame putative NLS and NES motifs between GFP and the SAR domain. (biomedcentral.com)
Moreover, analysis of a putative NLS located in the DBD ( 316 GQKKKNSN 323 ) by a similar GFP-SAR reporter or by internal deletion of the DBD, revealed this sequence to lack NLS activity. (biomedcentral.com)

This study identifies two nuclear localisation signals (NLSs) within BRCA2 and makes the important observation that they are downstream of all known cancer-associated truncating mutations in BRCA2 . (biomedcentral.com)
2014). A lot of FAKs features in tumor are via its part in signaling downstream of integrins and development factor receptors in the plasma membrane. (ufe-eg.org)
Primer 1/4 may also be introduced to this PCR assay for detection of some mutations close to the downstream EBE site, which could not be captured by Primer 2/3. (biomedcentral.com)
functions downstream of the cAMP/PKA signaling pathway and are novel transcriptional regulators associated with 192203-60-4 cellular differentiation during flower infection from the rice blast fungus. (bio2009.org)

In the early research, using bulk RNA sequencing of tumor samples from 7 PPGL patients, we found that PPGL tumor tissues exhibited high PD-L1 mRNA expression compared with adjacent normal adrenal medulla tissues, and this was related to T-cell exhaustion biomarkers. (bvsalud.org)
Abnormally activated Wnt/β-catenin signaling pathway plays a crucial role in promoting malignant tumor occurrence, development, recurrence, and metastasis, particularly in cancer stem cells. (frontiersin.org)
Localisation of hRad9 protein were performed on paired tumor and normal breast tissues. (biomedcentral.com)
Once an adrenal or extra-adrenal tumor is detected, CT scanning or MRI of the region may be performed for anatomic localization prior to surgical removal. (medscape.com)

Immunofluorescence of 293T cells, transfected with plasmids expressing GFP-tagged BRCA2 fragments, suggests that the C terminus of BRCA2 is responsible for nuclear translocation. (biomedcentral.com)
Here we show that GFP-ΔBRAF nuclear translocation does not involve a canonical Nuclear Localisation Signal (NLS), but is suppressed by N-terminal sequences. (bath.ac.uk)

Additionally, FUS/TLS has been found to bind a relatively long region in the 3′ untranslated region (UTR) of the actin-stabilising protein Nd1-L mRNA, suggesting that rather than recognising specific short sequences, FUS/TLS interacts with multiple RNA-binding motifs or recognises secondary conformations. (wikipedia.org)
Indeed, FUS/TLS interacts with several nuclear receptors. (wikipedia.org)
Rev interacts with Crm1 through a leucine-rich nuclear export signal located in its C-terminus, and with RRE through an Arginine rich motif (ARM) located in the N-terminal region. (nih.gov)
hRad9 is a nuclear protein that interacts with hRad1 and hHus1 to form a hetero-trimeric complex (the 9-1-1 complex) which is then loaded onto DNA [ 9 ]. (biomedcentral.com)

Different cancer types tend to depend on a limited number of 'driver' oncogene mutations. (cancerquest.org)

BRCA2 is localised to the nucleus and the functions ascribed to it are nuclear functions. (biomedcentral.com)
Nuclear GFP-ΔBRAF retains MEK/ERK activating potential and is associated with the accumulation of phosphorylated MEK and ERK in the nucleus. (bath.ac.uk)
The nuclear localization sequence (nls) of His4 was introduced into the N-terminal of Streptococcus pyogenes (SpCas9) to target the nucleus of the endonuclease SpCas9. (biomedcentral.com)
The signals of EYFP and DAPI (4′,6-diamidino-2-phenylindole) were co-localized in the nucleus of sporozoites (Figure 1 A). These results demonstrated that the nls of His4 efficiently localized SpCas9 to the nucleus. (biomedcentral.com)
The EYFP signal was located in the nucleus of the sporozoites, both after in vitro culture and in vivo propagation (sporozoites released from the sporulated oocyst was stained with DAPI and polyclonal antibodies against SAG13 as index of parasites nucleus and periphery). (biomedcentral.com)
It also contains a nuclear localization sequence (NLS) that targets the hRad9 protein into the nucleus [ 11 ]. (biomedcentral.com)
Presumably the C-terminal domain is essential for transport of the 9-1-1 complex from the cytoplasm into the nucleus, for activation of the G2 checkpoint signalling cascade [ 12 ]. (biomedcentral.com)
Prior to the first meiotic division, homologous chromosomes pair, synapse, and recombine to ensure their proper disjunction at anaphase I. Additionally, telomeres tethered at the nuclear envelope cluster in the bouquet configuration where they are subjected to dramatic pulling forces acting from outside of the nucleus. (biorxiv.org)
These attachment sites are linked to actin-bundles that surround the nucleus via an Ndj1-Mps3-Csm4 protein bridge that spans the inner and outer nuclear membranes. (biorxiv.org)

Wnt/β-catenin signaling is a delicate and complex signal transduction pathway mediated by multiple signaling molecules, which plays a significant role in regulating human physiology and pathology. (frontiersin.org)
MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)

The N-terminal ( IPR005398 ) portion of the protein is conserved neither in length nor sequence, but, in TUB, contains the nuclear localisation signal and may have transcriptional-activation activity. (embl.de)
However, recent studies have suggested that BRAFi/MEKi and ERK1/2i resistance can arise through activation of a parallel signalling pathway leading to activation of ERK5, an unusual protein kinase that contains both a kinase domain and a transcriptional transactivation domain. (babraham.ac.uk)
Studies have shown that the Wnt/β-catenin signaling pathway controls cell fate and function through the transcriptional and post-translational regulation of omics networks. (frontiersin.org)
The N-terminal region of SLR1 has two roles in GA signaling: interaction with GID1 and transactivation activity, and the suppressive function of the rice DELLA protein SLR1 is dependent on its transcriptional activation activity [7] . (ic4r.org)
ESE-1 initiates transformation of MCF-12A cells via a non-transcriptional, cytoplasmic process that is mediated by a unique 40-amino acid serine and aspartic acid rich (SAR) subdomain, whereas, ESE-1's nuclear transcriptional property is required to maintain the transformed phenotype of MCF7, ZR-75-1 and T47D breast cancer cells. (biomedcentral.com)
While previous publications have established ESE-1's transcription factor function, we have reported that ESE-1 initiates transformation of MECs via a novel non-nuclear, non-transcriptional mechanism [ 13 ]. (biomedcentral.com)

The cyclic AMP-dependent protein kinase A signaling pathway plays a major role in regulating plant infection from the rice blast fungus and or are both necessary for development of spores and appressoria by and play roles in cell wall differentiation, regulating melanin pigmentation and cell surface hydrophobicity during spore formation. (bio2009.org)

Pathogenic TDP-43 gene (TARDBP) mutations have been identified in familial ALS kindreds, and here we report a TARDBP variant (A90V) in a FTLD/ALS patient with a family history of dementia. (elsevierpure.com)
TDP-43 is a 43 kDa heterogeneous nuclear ribonuclear protein (hnRNP) composed of 414 amino acids and is encoded by the TARDBP gene located on chromosome 1 (1p36.22) [ 14 ]. (biomedcentral.com)

The ARM is a highly specific sequence which contains a nuclear localization signal. (nih.gov)

Furthermore, the Wnt/β-catenin can crosstalk with other signaling pathways including Notch, FGF, Hedgehog, and TGF-β/BMP signaling cascades to form a signaling network to regulate the survival and progression of cancer cells ( 14 - 16 ). (frontiersin.org)

Tubby, an autosomal recessive mutation, mapping to mouse chromosome 7, was recently found to be the result of a splicing defect in a novel gene with unknown function. (embl.de)

Notably, Debcl modulates Drp1 mitochondrial localization during apoptosis. (sdbonline.org)
Lysine-71 in the large delta antigen of hepatitis delta virus clade 3 modulates its localization and secretion. (cgu.edu.tw)

Germ-line mutations in the breast cancer susceptibility gene BRCA2 predispose carriers to breast cancer. (biomedcentral.com)

From a series of 20,000 full sequence tests of BRCA1 carried out at Myriad Genetic Laboratories, a total of 314 different missense changes and eight in-frame deletions were observed. (xenbase.org)
METHODS: We have used a combination of a multiple sequence alignment of orthologous BRCA1 sequences and a measure of the chemical difference between the amino acids present at individual residues in the sequence alignment to classify missense variants and in-frame deletions detected during mutation screening of BRCA1 . (xenbase.org)
Mutations, amplification and deletions of the upstream regulators of AKT are among the most frequent somatic events in cancer [ 2 , 3 ]. (oncotarget.com)

The phosphatidylinositol 3 kinase (PI3K)-AKT signaling cascade is a vital mediator of essential cellular activities including proliferation and survival [ 1 ]. (oncotarget.com)

Sequence analysis of BRCA2 revealed the presence of four potential nuclear localisation signals (NLSs), three near the C terminus. (biomedcentral.com)
First, I focused on functional characterization of the NLS1 and NLS2 domains as potential nuclear localization signals in TWIST. (uni-marburg.de)

We find that AKT phosphorylation of NPM-Ser48 prevents oligomerization that results in nucleoplasmic localization of ARF, constitutive MDM2 inhibition and stabilization of p53. (oncotarget.com)
Lee SL, Thomas P , Fenech M. Extracellular amyloid beta 42 causes necrosis, inhibition of nuclear division, and mitotic disruption under both folate deficient and folate replete conditions as measured by the cytokinesis-block micronucleus cytome assay. (academictree.org)
We observed that PIP, DUSP6, S100A8, and FOXA1 expression had been consis tently diminished from the inhibition of AR and ERK as well since the mixed inhibition of these two signaling path strategies in each cell lines. (cdantigens.com)
PIP expression is extremely regulated by AR ERK signaling We observed that PIP expression was consistently lowered following the inhibition of AR ERK signaling with a fold change of 0. (cdantigens.com)

C) Enlargement of part of the DBD showing the amino acid sequence (single letter codes) of the 2 zinc fingers and the dimerization loop (in bold). (medscape.com)
The amino acid sequence for this part of the protein resembled that of a human cytokine, EMAP II, which recruits circulating immune cells in tissues to promote inflammation. (fascea.pics)
Amino acid substitution during functionally constrained divergent evolution of protein sequences. (xenbase.org)
Characterization of disease-associated single amino acid polymorphisms in terms of sequence and structure properties. (xenbase.org)
It has a 17-amino acid deletion affecting the DELLA region, which results in a loss-of-function mutation of the SLR1 gene, which is an ortholog of RHT-1Da in wheat, D8 in maize, and GAI and RGA in Arabidopsis [3] . (ic4r.org)
In particular, TWIST NLS1 altered at amino acid position K38R was found to be retained in the cytoplasm of transiently transfected U2-OS cells, suggesting that NLS1 is functional and essential for the nuclear transport of TWIST. (uni-marburg.de)
Additionally, to understand the role of the TWIST NLS2 in nuclear localization, amino acid at positions 73, 76 and 77 were substituted in this motif. (uni-marburg.de)

METHODS: Here we combined bulk RNA sequencing, single-cell RNA sequencing, ProcartaPlex multiple immunoassays, functional experiments, and TissueFAXS panoramic tissue quantification assays to demonstrate that LRFN2 shapes a non-inflammatory TME in BLCA. (bvsalud.org)

Significantly, A90V is located between the bipartite nuclear localization signal sequence of TDP-43 and the in vitro expression of TDP-43-A90V led to its sequestration with endogenous TDP-43 as insoluble cytoplasmic aggregates. (elsevierpure.com)

Slr1-1 mutant which shows a slender phenotype is caused by a single recessive mutation and results in a constitutive GA response phenotype. (ic4r.org)

The study of mutant indicates that SLR1 is a negative regulator in GA signaling pathway, and overexpressing SLR1 gene in transgenic rice plants cause dwarf phenotype [1] . (ic4r.org)

TAR DNA-binding protein-43 (TDP-43) is a highly conserved, ubiquitously expressed nuclear protein that was recently identified as the disease protein in frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U) and amyotrophic lateral sclerosis (ALS). (elsevierpure.com)

The vast majority of these mutations are predicted to lead to the production of a truncated BRCA2 protein, lacking the C terminus. (biomedcentral.com)
Western blots of nuclear and cytoplasmic fractions of BRCA2 mutant cells. (biomedcentral.com)
Mice homozygous for some truncating mutations of Brca2 suffer embryonic lethality. (biomedcentral.com)
RAD51, a nuclear protein, has been shown to interact with the 6174delT form of BRCA2, which lacks the NLSs and is localised to the cytoplasm. (biomedcentral.com)
Spain BH, Larson CJ, Shihabuddin LS, Gage FH, Verma IM: Truncated BRCA2 is cytoplasmic: Implications for cancer-linked mutations. (biomedcentral.com)
Sequence analysis of BRCA1 and BRCA2: correlation of mutations with family history and ovarian cancer risk. (xenbase.org)

The corresponding plasmids were then sequenced to verify TALEN-induced mutations. (biomedcentral.com)

Mutations in Cu-ATPases, a subfamily of ATPases, are associated with Menkes and Wilson's diseases in humans. (nih.gov)

In 2009 two separate research groups analysed 26 unrelated families who presented with a type6 ALS phenotype, and found 14 mutations in the FUS gene. (wikipedia.org)

BRAF is a cytoplasmic protein kinase, which activates the MEK-ERK signalling pathway. (bath.ac.uk)

Computed tomography (CT) scanning and magnetic resonance imaging (MRI) have higher sensitivity in detecting pheochromocytomas than nuclear medicine scanning with iodine-131 metaiodobenzylguanidine ( 131 I-MIBG), although 131 I-MIBG uptake is more specific. (medscape.com)
Luster et al investigated the specificity and sensitivity of (18)F-3,4-dihydroxyphenylalanine (DOPA) positron emission tomography (PET) scanning, CT scanning, and a combination of the 2 modalities in the identification and localization of adrenal and extra-adrenal pheochromocytomas. (medscape.com)

It is caused by nucleotide sequence changes within or in proximity of the TWIST1 gene. (uni-marburg.de)

Signaling is initiated when the Wnt ligand binds to the Frizzled receptor on the cell membrane and the LDL receptor-associated protein 5/6 (LRP5/6) co-receptor. (frontiersin.org)

2 Protein interactions are critical to the stability, localization and activity of b-catenin, and we recently performed the first proteomic analyses of the b-catenin interactome in myeloid cells. (haematologica.org)
Our results show that the clinical candidate AKT inhibitor MK-2206 promotes ARF nucleolar localization, reduced p53 mut stability and increased sensitivity to ionizing radiation in a xenograft model of pancreatic cancer. (oncotarget.com)
In contrast to the stability of the epitope tags, the SV40 NLS sequence was rapidly mutated by a frameshift mutation that introduced negatively charged amino acids into the basic NLS. (researchwithrutgers.com)
Pathogenic Signal Sequence Mutations in Progranulin Disrupt SRP Interactions Required for mRNA Stability. (academictree.org)

b-Catenin is the central mediator of Wnt signaling and is frequently overexpressed in AML in which it is associated with poor prognosis. (haematologica.org)

This suggests the possibility that strictly regulated self-renewal mediated by Wnt signaling in cancer cells may be disturbed by the SUMOylation pathway to allow more malignant proliferation. (frontiersin.org)

Deregulation of the pathway is associated with the presence of BRAF mutations in human cancer, the most common being V600E BRAF, although structural rearrangements, which remove N-terminal regulatory sequences, have also been reported. (bath.ac.uk)
Melanoma in particular exhibits a high incidence of activating BRAF and NRAS mutations and such cells are addicted to the activity of these mutant oncoproteins. (babraham.ac.uk)

A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). (nih.gov)
We subse- the mechanism of influenza viral infection and replication quently used 15,785 protein sequences from the National in different host species. (cdc.gov)

Each TALEN monomer consists of a nuclear location signal (NLS), 152 amino acids deletion N-terminal, 63 amino acids from C-terminal, the TALE repeat domains, and modified Fok I nuclease domain ELD/KKR. (biomedcentral.com)

Specifically the effects of various NLS substitutions in TWIST on cellular localization was assayed by immunoflourescence assay. (uni-marburg.de)
C ) PCR assay determining indel mutations induced by TALENs. (biomedcentral.com)

RNA-binding protein FUS/TLS (FUsed in Sarcoma/Translocated in LipoSarcoma), also known as heterogeneous nuclear ribonucleoprotein P2 is a protein that in humans is encoded by the FUS gene. (wikipedia.org)

Using a hybrid positional cloning and next generation sequencing approach, we identified mutations in the nuclear localisation signal (NLS) of T-box transcription factor 4 (Tbx4) that impair nuclear localisation of the protein, resulting in altered gene expression patterns during pelvic fin development and the failure of pelvic fin development. (monash.edu)
Here, we record a function for nuclear FAK in regulating transcription of inflammatory chemokines and cytokines, in turn advertising an immuno-suppressive, pro-tumorigenic microenvironment. (ufe-eg.org)
Using the entire coding sequence of TWIST1 gene, an interesting candidate was found belonging to the class A bHLH transcription factors that includes the SEF2 gene product. (uni-marburg.de)
In a PCR array analysis of 84 transcription factors, either overexpressing DEFB1 or siRNA silencing of DEFB1 expression significantly modulated the expression of signal transducer and activator of transcription 3 (STAT3). (cdc.gov)

As shown in Figure 1F-H, the b-catenin:WT1 interaction remained in K562, HEL and KG-1 cells under both basal (DMSO) and stimulated (CHIR99021) Wnt signaling. (haematologica.org)
A new HIV-1 Rev structure optimizes interaction with target RNA (RRE) for nuclear export. (nih.gov)

It mainly resides in nuclear ribonucleoprotein granules but also undergoes nucleocytoplasmic shuttling to participate in cytoplasmic ribonucleoprotein granules10. (lafurnitura.com)

a GSK3b inhibitor), and naturally activated (with recom-binant rWNT3A) Wnt signaling conditions ( Figure 1C ). (haematologica.org)

Thus, A90V may be a genetic risk factor for FTLD/ALS because it predisposes nuclear TDP-43 to redistribute to the cytoplasm and form pathological aggregates. (elsevierpure.com)
RAF-MEK-ERK signalling is normally thought to occur in the cytoplasm of the cell. (bath.ac.uk)

However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)

cAMP is a signaling molecule important for a variety of cellular functions. (nih.gov)

These results demonstrated that substitution at NLS2 position 76 does not play an essential role in the nuclear localization of TWIST, in contrast to the K73R and K77R that inhibit nuclear accumulation. (uni-marburg.de)
These results suggest that a physical impediment to nuclear division is created in the absence of Nup2 and Ndj1. (biorxiv.org)

The instability of the NLS suggests that the strong nuclear localization of the IN-SV40 NLS may have detrimental effects on virus assembly. (researchwithrutgers.com)
Mutations in HELLS, its activator CDCA7, and the de novo DNA methyltransferase DNMT3B, cause immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome, a genetic disorder associated with the loss of DNA methylation. (elifesciences.org)

The nuclear localization sequences of the BRCA1 protein interact with the importin-alpha subunit of the nuclear transport signal receptor. (xenbase.org)
However, these problems in mutants could be complemented by adding exogenous cAMP or by spontaneous mutations in the regulatory subunit of PKA gene [44]. (bio2009.org)

3. Translational research - to identify predictive biomarkers for treatment decision - by immunohistochemistry, gene expression or sequencing. (lu.se)

abstract = "Moloney murine leukemia virus (M-MuLV) proviruses carrying integrase (IN) protein tagged either with a simian virus 40 (SV40) nuclear localization signal (NLS) or various antigenic epitopes were generated. (researchwithrutgers.com)

These resistance mechanisms frequently involve reinstatement of ERK1/2 signalling and BRAFi are now deployed in combination with one of three approved MEK1/2 inhibitors (MEKi) to provide more durable, but still transient, clinical responses. (babraham.ac.uk)

DNA methylation, particularly 5-methylcytosine (5mC) at CpG sequences, is widely conserved in eukaryotes. (elifesciences.org)

Our findings revise theories of labour division between the major RNA polymerases, and identify nucleolar Pol II as a major factor in protein synthesis and nuclear organization, with potential implications for health and disease. (nature.com)
These observations have implications for studying nuclear transport properties of M-MuLV and for engineering a murine-based retroviral vector for gene therapy. (researchwithrutgers.com)

Anatomy25

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Proteins9

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