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Escherichia coli ProteinsEscherichia coliEscherichia coli InfectionsMolecular Sequence DataBacterial ProteinsGenes, BacterialEscherichia coli O157Amino Acid SequencePlasmidsMutationBase SequenceCloning, MolecularDNA, BacterialEscherichia coli K12Gene Expression Regulation, BacterialGenetic Complementation TestKineticsMutant ProteinsEscherichiaOperonMutagenesis, Site-DirectedAdhesins, Escherichia coliPhenotypeSequence Homology, Amino AcidBinding SitesTemperatureChromosomes, BacterialRNA, BacterialGenesBacterial Outer Membrane ProteinsRestriction MappingProtein BindingTranscription, GeneticModels, MolecularMutagenesis, InsertionalRecombinant Fusion ProteinsRecombinant ProteinsCulture MediaProtein ConformationChromosome MappingGene DeletionSubstrate SpecificityMice, Mutant StrainsDrug Resistance, MicrobialColiphagesMembrane ProteinsElectrophoresis, Polyacrylamide GelProtein Structure, TertiaryCarrier ProteinsSequence AlignmentDNA PrimersAnti-Bacterial AgentsAmino Acid SubstitutionVirulenceSuppression, GeneticDNA Transposable ElementsConjugation, GeneticDNA-Binding ProteinsBacterial AdhesionStructure-Activity RelationshipRecombination, GeneticBacterial ToxinsGenetics, MicrobialSequence Analysis, DNAMembrane Transport Proteinsbeta-GalactosidaseFimbriae, BacterialTransduction, GeneticPromoter Regions, GeneticCell LineEnterotoxigenic Escherichia coliCell MembranePoint MutationMolecular WeightHydrogen-Ion ConcentrationShiga-Toxigenic Escherichia coliDNA ReplicationTranscription FactorsSequence DeletionAdenosine TriphosphatasesEnteropathogenic Escherichia coliEnterotoxinsPolymerase Chain ReactionSpecies SpecificitySalmonella typhimuriumHot TemperatureGenes, RegulatorChloramphenicolGene ExpressionDNA-Directed RNA PolymerasesCatalysisBacteriophage lambdaBiological TransportCysteineLac OperonEnterohemorrhagic Escherichia coliTransformation, BacterialRepressor ProteinsMicrobial Sensitivity TestsDNA Restriction Enzymes
Expressed in Escherichia coliStrainResistant mutantsOuter membrane proteinGenesMG1655Coli mutantEnzymeKlebsiellaMutationsBacterialStrainsMetabolicMetabolismProteinAlleleGeneticCharacterizationSdhCResiduesIsolationOperonExpressionEnzymesBacteriaCiprofloxacinVitroSynthesisPreventionResistanceExperimentalKineticGrowthRegulationBoundConcentrationAnalysisFoundSpecificFunctionsResponseProductionFactors
Expressed in Escherichia coli3
  • 2004. Aggregation of ALS mutant superoxide dismutase expressed in Escherichia coli. . (oregonstate.edu)
  • The violaxanthin de-epoxidase (VDE) gene from spinach (Spinacia oleracea) was cloned, sequenced (GenBank AJ 250433), and expressed in Escherichia coli. (lu.se)
  • article{939b7e14-4ce7-4517-a0a3-5bfc01c5e6af, abstract = {{The violaxanthin de-epoxidase (VDE) gene from spinach (Spinacia oleracea) was cloned, sequenced (GenBank AJ 250433), and expressed in Escherichia coli. (lu.se)
Strain6
  • A cmlB mutant accumulated tetracycline at a threefold] lower rate than the wild-type strain, and it is proposed that the mutants have an altered permeability to the drugs and that this acts syner-gistically with the products of the R factor chloramphenicol and tetracycline resistance genes. (microbiologyresearch.org)
  • Variations in resistance to three antibiotics among some single-step mutants to chloramphenicol resistance in a strain of Escherichia coli K12. (microbiologyresearch.org)
  • There is a significant reduction of adherence ability of papG mutant strain as compared to control observed through microscopy. (microbiologyresearch.org)
  • By comparing the growth patterns of prototypical enteropathogenic, uropathogenic, and nonpathogenic Escherichia coli strains devoid of σ 54 , we uncovered that the absence of σ 54 results in two differently sized colonies that appear at different times specifically in the uropathogenic E. coli (UPEC) strain. (nih.gov)
  • Notably, UPEC bacteria devoid of individual activator ATPases of the σ 54 -RNAP do not phenocopy the σ 54 mutant strain. (nih.gov)
  • The resultant pseudo F plasmid is transferred by conjugation to the newly established single gene deletion mutant and selected Hfr strain integrated F plasmid by antibiotic resistance selection. (cam.ac.uk)
Resistant mutants2
  • Plasmid-less derivatives of these moderately resistant mutants were phenotypically similar to the cmlB mutants described by Reeve (1966 , 1968) , and also mapped in the same region. (microbiologyresearch.org)
  • From the comparison of the kinetic parameters of transport of maltose and maltotriose in wild‐type and λ‐resistant mutants with the binding constants for both sugars to purified maltose‐binding protein, we conclude that the λ receptor facilitates the diffusion of maltose and maltodextrins through the outer membrane. (princeton.edu)
Outer membrane protein1
  • Modulations in lipid A and phospholipid biosynthesis pathways influence outer membrane protein assembly in Escherichia coli K-12. (wikigenes.org)
Genes1
MG16551
  • Characterization of E. coli MG1655 and frdA and sdhC mutants at various aerobiosis levels. (mpg.de)
Coli mutant2
  • In this work, we describe an EPR-detectable QFR semiquinone using Escherichia coli mutant QFR (FrdC E29L) and the wild-type enzyme. (lu.se)
  • However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)
Enzyme9
  • Steady-state kinetic analysis revealed that mutant enzymes exhibited k(cat) values 1.1-95-fold lower than that of the wild-type enzyme. (rcsb.org)
  • Analysis of pre-steady-state kinetics of ternary complex formation revealed that the productive binding of CH(2)H(4)folate is weaker to mutant TSs than to the wild-type enzyme. (rcsb.org)
  • Chemical transformation constants (k(chem)) for the mutant enzymes were lower by 1.1-6.0-fold relative to the wild-type enzyme. (rcsb.org)
  • E. coli QFR activity and the fast relaxing SQ species observed in the mutant enzyme are sensitive to the inhibitor 2-n-heptyl-4-hydroxyquinoline N-oxide (HQNO). (lu.se)
  • In the case of Pyk mutant, the enzyme level regulation was made clear such that Pyk knockout caused PEP concentration to be up-regulated and activated Ppc, which caused the increase in MAL concentration and backed up reduced PYR through Mez, resulting in the phenotypic growth characteristics similar to the wild type. (biomedcentral.com)
  • Balanced biosynthesis of major membrane components through regulated degradation of the committed enzyme of lipid A biosynthesis by the AAA protease FtsH (HflB) in Escherichia coli. (wikigenes.org)
  • In Escherichia coli branching enzyme, this residue (Y300) is located prior to the conserved region 1. (unboundmedicine.com)
  • TY - JOUR T1 - Tyrosine residue 300 is important for activity and stability of branching enzyme from Escherichia coli. (unboundmedicine.com)
  • To achieve enzyme-activatable cMOs, we focused on the Escherichia coli nitroreductase NfsB as the triggering catalyst. (cdc.gov)
Klebsiella3
Mutations3
Bacterial2
  • Mutant prevention concentration (MPC) is an in vitro test used to determine the lowest drug concentration needed to inhibit the growth of a single-step-mutant bacterial subpopulation. (ugm.ac.id)
  • The creation and isolation of specific mutants is a cornerstone of bacterial genetics. (lu.se)
Strains1
  • Here, we describe a simple and reproducible protocol of mouse model of infection to evaluate the attenuation of the genetically modified strains of Pseudomonas aeruginosa in comparison to the United States Food and Drug Administration (FDA)-approved Escherichia coli for commercial applications. (jove.com)
Metabolic3
  • It is preferred to model the whole of the main metabolic pathways in Escherichia coli , allowing for the estimation of energy generation and cell synthesis, based on intracellular fluxes and that may be used to characterize phenotypic growth. (biomedcentral.com)
  • Characterisation of metabolic mutants with respect to their growth behaviour with different oxygen availabilities. (mpg.de)
  • Model-Based metabolic engineering enables high yield itaconic acid production by Escherichia coli. (mpg.de)
Metabolism1
  • A mathematical model of metabolism and regulation provides a systems-level view of how Escherichia coli responds to oxygen. (mpg.de)
Protein1
  • These phenotypes appear to result, at least in part, from impaired interactions of the mutant β159 clamp protein with the replicative DNA polymerase, Pol III ( 38 ). (asm.org)
Allele2
  • The Escherichia coli dnaN159 allele encodes a mutant form of the β-sliding clamp (β159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. (asm.org)
  • The dnaN159 allele encodes a mutant form of the β-sliding clamp (β159) that bears two amino acid substitutions: G66E (glycine at position 66 replaced with glutamic acid) and G174A ( 14 , 35 , 38 ). (asm.org)
Genetic2
  • METHODOLOGY/PRINCIPAL FINDINGS: Genetic analysis of two recA(C) mutants was used to determine the mechanism of constitutive SOS (SOS(C)) expression in a population of log phase cells using fluorescence of single cells carrying an SOS reporter system (sulAp-gfp). (umass.edu)
  • Genetic analysis of flagellar mutants in Escherichia coli. (yale.edu)
Characterization1
  • Barbé, J , Guerrero, R & Villaverde, A 1983, ' Further characterization of the expression of SOS functions in recA430 mutants of Escherichia coli ', Mutation Research , vol. 121, no. 3-4, pp. 171-175. (uab.cat)
SdhC1
  • The membrane anchor of SQR in mammalian mitochondria and proteobacteria, such as Escherichia coli , consists of two polypeptides (SdhC and SdhD) and contains one heme group. (lu.se)
Residues1
  • To examine the role of water penetration, we have measured the 17O and 2H magnetic relaxation dis- persions (MRD) for the V66E and V66K mutants of staphylococcal nuclease, where glutamic acid and lysine residues are buried in predominantly apolar environments. (lu.se)
Isolation1
Operon1
  • Transcription of the aml operon is significantly repressed in the wild type when growing on glucose and repression is absent in an Δ amlR deletion mutant. (frontiersin.org)
Expression5
  • Differential requirements of two recA mutants for constitutive SOS expression in Escherichia coli K-12. (umass.edu)
  • SOS(C) expression in recA4142 mutants was dependent on its initial level of transcription, recBCD, recFOR, recX, dinI, xthA and the type of medium in which the cells were grown. (umass.edu)
  • The VHH (variable domain of heavy chain antibody) gene of Nb28 was subcloned into the expression vector pecan45 containing the AP double-mutant gene. (cdc.gov)
  • The Nb28-AP construct was transformed into Escherichia coli BL21(DE3)plysS, and soluble expression in bacteria was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot. (cdc.gov)
  • Chromosomal AmpC expression can become induced by certain antibacterials or may become constitutively expressed (ie, overproduced) in de-repressed mutants. (msdmanuals.com)
Enzymes3
  • Relative to wild-type TS, K(m) values of the mutant enzymes for 2'-deoxyuridylate (dUMP) were 5-90 times higher, while K(m) values for 5,10-methylenetetrahydrofolate (CH(2)H(4)folate) were 1.5-16-fold higher. (rcsb.org)
  • The rate of dehalogenation of 5-bromo-2'-deoxyuridine 5'-monophosphate (BrdUMP), a reaction catalyzed by TS that does not require CH(2)H(4)folate as cosubstrate, by mutant TSs was analyzed and showed that only S167A and S167G catalyzed the dehalogenation reaction and values of k(cat)/K(m) for the mutant enzymes were decreased by 10- and 3000-fold, respectively. (rcsb.org)
  • Replacement of Y300 with Ala, Asp, Leu, Ser, and Trp resulted in mutant enzymes with less than 1% of wild-type activity. (unboundmedicine.com)
Bacteria1
Ciprofloxacin2
  • The purpose of this study was to determine the MPC value of ciprofloxacin against pathogenic Escherichia coli to obtained the range of mutant selection windows (MSW) of ciprofloxacin. (ugm.ac.id)
  • Based on the results of this MPC assessment it can be concluded that the dose of ciprofloxacin in production animals has a wide range of MSW that is allow for single-step mutants. (ugm.ac.id)
Vitro1
Synthesis1
  • Regulatory mechanism for synthesis of capsular polysaccharide in mucoid mutants of Escherichia coli K-12. (yale.edu)
Prevention1
  • New concepts in antimicrobial susceptibility testing: the mutant prevention concentration and mutant selection window approach. (ugm.ac.id)
Resistance4
Experimental1
Kinetic2
Growth2
  • The slower growth rate of the Ppc mutant was properly estimated by taking into account the lower specific ATP production rate. (biomedcentral.com)
  • Enabling anaerobic growth of Escherichia coli on glycerol in defined minimal medium using acetate as redox sink. (mpg.de)
Regulation1
  • Kinase activity of ArcB from Escherichia coli is subject to regulation by both ubiquinone and demethylmenaquinone. (mpg.de)
Bound2
  • Notably, certain B. subtilis mutants with mutated Fp lack covalently bound flavin and do not assemble the SQR polypeptides in the membrane [3]. (lu.se)
  • The slight difference in electrophoretic mobility of normal Fp and that produced in E. coli could be due to the absence of covalently FAD but could have another explanation because this difference was not observed for some mutant Fp without covalently bound FAD [3]. (lu.se)
Concentration1
  • It was shown to be difficult for the cell to grow in Ppc mutant due to low concentration of OAA, while Pck mutant does not necessarily show this phenomenon. (biomedcentral.com)
Analysis1
Found1
  • Escherichia coli (/ˌɛʃəˈrɪkiə ˈkoʊlaɪ/ ESH-ə-RIK-ee-ə KOH-ly) is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms. (wikipedia.org)
Specific1
  • Enforced ATP futile cycling increases specific productivity and yield of anaerobic lactate production in Escherichia coli. (mpg.de)
Functions1
  • The Escherichia coli dnaN -encoded β-sliding clamp functions as a homodimer and is "loaded" onto primed DNA by the multisubunit DnaX clamp loader complex ( 5 , 21 ). (asm.org)
Response1
Production1
  • Temperature-dependent dynamic control of the TCA cycle increases volumetric productivity of itaconic acid production by Escherichia coli. (mpg.de)
Factors1