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Chlamydomonas reinhardtiiChlamydomonasAlgal ProteinsChloroplastsFlagellaRNA, AlgalCytochromes fPhotosynthesisDNA, AlgalChlorophytaCytochrome b6f ComplexPhotosystem II Protein ComplexMolecular Sequence DataChlorophyllLightPhotosystem I Protein ComplexPhotosynthetic Reaction Center Complex ProteinsPlant ProteinsAmino Acid SequenceGenes, ProtozoanMutationPlastocyaninThylakoidsLight-Harvesting Protein ComplexesAxonemeProtozoan ProteinsTransformation, GeneticArylsulfatasesDiuronDyneinsAmylopectinSequence Homology, Amino AcidBase SequencePlastoquinoneDarknessGenetic Complementation TestVolvoxRibulose-Bisphosphate CarboxylaseSulfurCytochromes c6RNA, ChloroplastMicroalgaeCloning, MolecularChloroplast ProteinsDNA, ChloroplastHydrogenasePhenotypeCarbonic AnhydrasesCytochrome b GroupElectron TransportMutagenesis, InsertionalMalate Dehydrogenase (NADP+)KineticsStarchCarbon DioxideGenes, PlantSequence AlignmentHydrogenCytochromesOxidation-ReductionMutagenesis, Site-DirectedGene Expression Regulation, PlantStarch SynthasePhylogenyChlorellaGeranylgeranyl-Diphosphate GeranylgeranyltransferaseFerredoxinsRNA, MessengerOrganelle SizeElectrophoresis, Polyacrylamide GelMicrotubulesRNA, ProtozoanPhototrophic ProcessesEscherichia coliMutant ProteinsIsoamylaseOxygenTemperatureCrosses, GeneticFluorescenceDNA, ComplementaryGenesPyruvate SynthaseCiliaMolecular WeightRestriction MappingCoproporphyrinogen OxidaseThioredoxin hModels, MolecularMicroscopy, ElectronDNA, ProtozoanAnaerobiosisEukaryotaTranscription, GeneticIron-Sulfur ProteinsSuppression, GeneticProtein BiosynthesisRNA, PlantTubulinCell Nucleus
AlgaPhotosystemDeficientChloroplastAbstractGeneticsMitochondrialFlagellarCytochromeChlorophyllMicroalgaProteinPhotosynthesisInsertionalPhotosyntheticOuter dyneinThylakoidMolecularKineticsStrainStrainsDyneinCellsGenome sequenceSpeciesNuclearProtegensArabidopsisGreenProteinsGene expressionPhotoautotrophicallyChromatinMetabolicBiomassSurvivalMetabolismSignificantlyExhibitStudyReveals
Alga10
  • Chlamydomonas reinhardtii, a unicellular green alga, often experiences hypoxic/anoxic soil conditions that activate fermentation metabolism. (montana.edu)
  • Chlamydomonas reinhardtii is a single-cell green alga about 10 micrometres in diameter that swims with two flagella. (wikipedia.org)
  • Therefore, the aim of this study was to define a specific role for ABA in the motile green alga, Chlamydomonas reinhardtii , providing evidence that this phytohormone mediates the light-dependent diurnal rhythm of up and down gravitaxis of the algae in the water column. (nature.com)
  • An epigenetic gene silencing pathway selectively acting on transgenic DNA in the green alga Chlamydomonas. (mpg.de)
  • Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii. (mpg.de)
  • The research, performed mostly by Graham Peers in the laboratory of Krishna Niyogi from the University of California, Berkeley, included researchers at the University of Münster, Germany, and used a mutant strain of the single-celled green alga Chlamydomonas reinhardtii, originally isolated at the Carnegie Institution, to show that a specific protein of the light harvesting family of proteins plays a critical role in eliminating excess absorbed light energy. (scienceblog.com)
  • Here, we characterised physiological and proteomic changes between a low-starch C. reinhardtii strain and the snow alga Chlamydomonas nivalis, to reveal insights into their contrasting responses to salinity stress. (biomedcentral.com)
  • Several aspects of the photoresponse have previously been analyzed in volvocine algae, particularly in the unicellular alga Chlamydomonas. (uni-bielefeld.de)
  • The sites of synthesis of the major thylakoid membrane polypeptides have been studied in the green alga Chlamydomonas reinhardtii by pulse labeling of cells with [14C]acetate in the presence of inhibitors specific for chloroplast and cytoplasmic protein synthesis. (rupress.org)
  • The unicellular alga Chlamydomonas reinhardtii and the bacterium Pseudomonas protegens serve as a model to study the interactions between photosynthetic and heterotrophic microorganisms. (leibniz-hki.de)
Photosystem6
Deficient2
Chloroplast4
  • Conditional Depletion of the Chlamydomonas Chloroplast ClpP Protease Activates Nuclear Genes Involved in Autophagy and Plastid Protein Quality Control. (mpg.de)
  • Chloroplast DnaJ-like proteins 3 and 4 (CDJ3/4) from Chlamydomonas reinhardtii contain redox-active Fe-S clusters and interact with stromal HSP70B. (mpg.de)
  • Further characterization of Trp-14 using chloroplast transformation in Chlamydomonas indicated that substitution of D1 Trp-14 to Phe, mimicking Trp oxidation enhanced FtsH-mediated D1 degradation under high light, although the substitution did not affect protein stability and PSII activity. (elifesciences.org)
  • Experiments with a mutant whose chloroplast ribosomes are resistant to spectinomycyn (spr-u-1-6-2) show that polypeptides whose synthesis takes place on chloroplast ribosomes are made in the presence of spectinomycin in the mutant although their synthesis is blocked by this antibiotic in wild type cells. (rupress.org)
Abstract1
  • abstract = "Chlamydomonas reinhardtii swims toward or away from light (phototaxis) in a graded way depending on various conditions. (syr.edu)
Genetics2
  • Chlamydomonas reinhardtii is an especially well studied biological model organism, partly due to its ease of culturing and the ability to manipulate its genetics. (wikipedia.org)
  • Genetics and Gene are inextricably linked to his Chlamydomonas reinhardtii research. (research.com)
Mitochondrial2
  • The genome of C. reinhardtii is significant for mitochondrial study as it is one species where the genes for 6 of the 13 proteins encoded for the mitochondria are found in the nucleus of the cell, leaving 7 in the mitochondria. (wikipedia.org)
  • The deduced primary structure of subunit I from cytochrome c oxidase suggests that the genus Polytomella shares a common mitochondrial origin with Chlamydomonas. (bio.net)
Flagellar3
  • These mutants are useful tools for studying a variety of biological processes, including flagellar motility, photosynthesis, and protein synthesis. (wikipedia.org)
  • In the Chlamydomonas reinhardtii flagellar axoneme, at least 11 dynein heavy chains (three outer arm and eight inner arm) exist, and each could play crucial and distinct roles in proper flagellar functions ( Kagami and Kamiya, 1992 ). (rupress.org)
  • We analyzed two Chlamydomonas reinhardtii mutants, mia1 and mia2, which display slow swimming and low flagellar beat frequency. (emory.edu)
Cytochrome1
Chlorophyll2
Microalga2
  • These differences in protein abundance have given greater understanding of the mechanism by which salt stress promotes fatty acid accumulation in the un-sequenced microalga C. nivalis as it switches to a non-growth state, whereas C. reinhardtii does not have this response. (biomedcentral.com)
  • The bacterium Pseudomonas protegens antagonizes the microalga Chlamydomonas reinhardtii using a blend of toxins. (uni-leipzig.de)
Protein4
Photosynthesis2
  • Systems Analysis of the Response of Photosynthesis, Metabolism, and Growth to an Increase in Irradiance in the Photosynthetic Model Organism Chlamydomonas reinhardtii. (mpg.de)
  • Photosynthesis and respiration rates are reduced in C. reinhardtii relative to C. nivalis in response to 0.2 M NaCl. (biomedcentral.com)
Insertional1
Photosynthetic4
  • Reasoning that high-light-induced photo-oxidation likely poses the main threat to photosynthetic algae, here the initial investigation focused on the effect of ABA on the photosynthetic efficiency of C. reinhardtii by measuring the ability of treated algae to deplete their growing media of dissolved HCO 3 − under different light levels. (nature.com)
  • Systems-Wide Analysis of Acclimation Responses to Long-Term Heat Stress and Recovery in the Photosynthetic Model Organism Chlamydomonas reinhardtii. (mpg.de)
  • Nitrogen‐Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism. (mpg.de)
  • Evidence for a Role of VIPP1 in the Structural Organization of the Photosynthetic Apparatus in Chlamydomonas. (mpg.de)
Outer dynein2
  • Two functional thioredoxins containing redox-sensitive vicinal dithiols from the Chlamydomonas outer dynein arm. (bio.net)
  • In axonemes from mutants that lack both the outer dynein arms and the MIA complex, I1 dynein failed to assemble, suggesting physical interactions between these three axonemal complexes and a role for the MIA complex in the stable assembly of I1 dynein. (emory.edu)
Thylakoid1
Molecular3
  • Chlamydomonas is used as a model organism for research on fundamental questions in cell and molecular biology such as: How do cells move? (wikipedia.org)
  • The majority of molecular research in this area has focused on Chlamydomonas reinhardtii . (biomedcentral.com)
  • The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. (research.com)
Kinetics2
Strain4
  • This strain, like the pfl1 mutants, secreted lactate, but it also exhibited a significant increase in the levels of extracellular glycerol, acetate, and intracellular reduced sugars and a decrease in dark, fermentative H2 production. (montana.edu)
  • The C. reinhardtii wild-type laboratory strain c137 (mt+) originates from an isolate collected near Amherst, Massachusetts, in 1945 by Gilbert M. Smith. (wikipedia.org)
  • The effect of 100 mM NaCl salt stress on lipid content in wild-type CC124 C. reinhardtii has revealed a 50-fold increase from approximately 0.1 µg TAG 10 -6 cells to approximately 5 µg TAG 10 -6 cells [ 9 ], to reach similar levels to those found for this strain under nitrogen deprivation. (biomedcentral.com)
  • I performed a metal(loid)s resistance assay for both strains using the wild type strain C. reinhardtii 950 as control. (edu.hk)
Strains2
  • In the lead up to the Artemis-1 launch, Nislow and his team devised a payload of 6,000 mutant yeast strains. (nsnews.com)
  • Two mutant strains, Cds18 and CdrB2 with Cd sensitive and resistance respectively, were generated by insertational mutagensis in our laboratory previously. (edu.hk)
Dynein2
  • We used cryo-electron tomography to reconstruct the three-dimensional structure of inner dynein arms from Chlamydomonas reinhardtii . (rupress.org)
  • Cryo-electron tomography of mia mutant axonemes revealed that the MIA complex was located immediately distal to the intermediate/light chain complex of I1 dynein and structurally appeared to connect with the nexin-dynein regulatory complex. (emory.edu)
Cells3
  • Vegetative cells of reinhardtii species are haploid with 17 small chromosomes. (wikipedia.org)
  • Nonphotochemical quenching (NPQ) induction in low-light (LL) acclimated Chlamydomonas reinhardtii cells. (elifesciences.org)
  • P. protegens secretes the cyclic lipopeptide orfamide A that interferes with cytosolic Ca 2+ homeostasis in C. reinhardtii resulting in deflagellation of the algal cells. (leibniz-hki.de)
Genome sequence2
  • In 2007, the complete nuclear genome sequence of C. reinhardtii was published. (wikipedia.org)
  • The Chlre3 draft of the Chlamydomonas nuclear genome sequence prepared by Joint Genome Institute of the U.S. Dept of Energy comprises 1557 scaffolds totaling 120 Mb. (wikipedia.org)
Species4
  • Chlamydomonas species are widely distributed worldwide in soil and fresh water. (wikipedia.org)
  • The species' name has been spelled several different ways because of different transliterations of the name from Russian: reinhardi, reinhardii, and reinhardtii all refer to the same species, C. reinhardtii Dangeard. (wikipedia.org)
  • Since Chlamydomonas species are normally haploid, the effects of mutations are seen immediately without further crosses. (wikipedia.org)
  • In this study, genes related to heavy metal responses in Chlamydomonas reinhardtii, a unicellular green algae model species with its entire genome sequenced, were characterized. (edu.hk)
Nuclear2
Protegens1
  • Here, we studied the roles of additional secondary metabolites secreted by P. protegens using individual compounds and co-cultivation of algae with bacterial mutants. (leibniz-hki.de)
Arabidopsis2
  • We revealed that Arabidopsis mutant lacking FtsH2 had increased levels of oxidative Trp residues in D1, among which an N-terminal Trp-14 was distinctively localized in the stromal side. (elifesciences.org)
  • The heterologous expression of HpDGAT2D in Arabidopsis thaliana and Chlamydomonas reinhardtii significantly increased the TAG content and obviously promoted the MUFAs and PUFAs contents. (researchsquare.com)
Green1
  • Synthesis of lurlene, the sex pheromone of the green flagellate Chlamydomonas allensworthii. (bio.net)
Proteins1
  • Channelrhodopsin-1 and Channelrhodopsin-2, proteins that function as light-gated cation channels, were originally isolated from C. reinhardtii. (wikipedia.org)
Gene expression1
  • 2019). Regulation of photoprotection gene expression in Chlamydomonas by a putative E3 ubiquitin ligase complex and a homolog of CONSTANS. (agrisera.com)
Photoautotrophically1
  • When illuminated, C. reinhardtii can grow photoautotrophically, but it can also grow in the dark if supplied with organic carbon. (wikipedia.org)
Chromatin3
Metabolic2
  • Although the metabolic adjustments observed in the mutants facilitate NADH reoxidation and sustained glycolysis under dark, anoxic conditions, the observed changes could not have been predicted given our current knowledge of the regulation of fermentation metabolism. (montana.edu)
  • Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts. (mpg.de)
Biomass1
Survival1
Metabolism1
  • Co-cultivation with a regulatory mutant of bacterial secondary metabolism (ΔgacA) promotes algal growth under spatially structured conditions. (leibniz-hki.de)
Significantly1
  • We show that exogenous ABA significantly altered the HCO 3 − uptake of Chamydomonas reinhardtii in a light-intensity-dependent manner. (nature.com)
Exhibit1
  • ABA loss of function mutants exhibit pleiotropic phenotypes and simply applying ABA to plants induces an immediate stress response. (nature.com)
Study1
Reveals2
  • Dissecting the Heat Stress Response in Chlamydomonas by Pharmaceutical and RNAi Approaches Reveals Conserved and Novel Aspects. (mpg.de)
  • Quantitative shotgun proteomics using a uniform 15N-labeled standard to monitor proteome dynamics in time course experiments reveals new insights into the heat stress response of Chlamydomonas reinhardtii. (mpg.de)