The genes that encode the main porins in K. pneumoniae, ompK35 and ompK36, and several beta-lactamase genes were analyzed. (bvsalud.org)
Functional characterization of KPC-109 was performed investigating the impact of KPC-109 production on the β-lactam resistance phenotype of various Escherichia coli and Klebsiella pneumoniae strains, including derivatives of K. pneumoniae with OmpK35 and OmpK36 porin alterations. (imperial.ac.uk)
Strains that lack the outer membrane porins OmpK35 and OmpK36, which allow passive diffusion of small hydrophilic molecules, exhibit antibiotic resistance. (biobanking.com)
Only four clinical isolates with OmpK35 and OmpK36 porin defects and carrying multiple beta lactamases and carbapenemases had raised MICs against ceftaroline/avibactam, 86% were successfully inhibited despite the porin defects and β-lactamases. (biobanking.com)
Proteins5
In addition, mutation or altered and OmpF porin proteins, and 1 showed decreased pro- expression of outer membrane proteins constituting porins duction of both. (cdc.gov)
The purified mutant proteins were reconstituted into lipid bilayer membranes and the single-channel conductance was studied. (uni-wuerzburg.de)
Virulome analysis of M-12 isolate revealed the presence of the following factors: siderophores, heme uptake systems, capsule synthesis, curli and type I fimbriae, flagella proteins, OmpA porin, etc. (bvsalud.org)
The monomeric porin OmpG is especially attractive compared with multisubunit proteins because appropriate modifications of the pore can be easily achieved by mutagenesis. (ox.ac.uk)
T ations may protect E. coli and allow subsequent selection he development of antibiotic resistance in Escherichia for promoter and attenuator mutants. (cdc.gov)
Here we report the effects of the mutation of two different aspartate residues of Escherichia coli OmpC porin on the polyamine sensitivity of the channel. (tamu.edu)
The Escherichia coli protein SurA which has been characterized as a periplasmic peptidyl-prolyl-isomerase (PPIase) and chaperon is involved in the maturation of trimeric outer membrane porins. (uni-goettingen.de)
Coli3
In the last part the influence of point mutants within the greasy slide of the maltose specific porin (LamB) of E. coli were shown. (uni-wuerzburg.de)
E. coli nonspecifically transports glucose from the extracellular medium into the periplasmic space through the outer membrane porins. (encyclopedia.pub)
Whole-cell studies with a decisive set of E. coli porin mutants provide evidence that translocation of kanamycin via porins is relevant for antibiotic potency. (chemrxiv.org)
Strains4
Porin mutants may occur more commonly and be less fit and less inclined to spread knowledge, no investigators have concurrently looked for or cause disease than strains with increased -lactamase alterations in porins in addition to promoter-attenuator expression. (cdc.gov)
Comparative virulome analysis among available E. marmotae genomes revealed the presence of capsule K1 genes mostly in pathogenic isolates and OmpA porin presence among all strains. (bvsalud.org)
Ceftaroline showed potent activity against K. pneumoniae ATCC 13883 and all the mutant porin strains including double deletion mutants with a minimum inhibitory concentration (MIC) range of 0.125-0.25 µg/ml. (biobanking.com)
The various effects of the above mentioned antibiotics and inhibitors were also tested on strains carrying mutant derivatives of the Col VIK-94. (ucl.ac.uk)
Alterations3
Porin alterations might work together to pro- duce a higher level of resistance. (cdc.gov)
However, in the presence of porin alterations commonly encountered in high-risk clonal lineages of K. pneumoniae, KPC-109 was able to confer clinical-level resistance to carbapenems as well. (imperial.ac.uk)
However, avibactam diffusion into the bacteria was not influenced by the presence of different porin alterations, consistent with earlier findings, making this drug combination a potentially effective therapeutic agent to treat infections caused by multidrug-resistant bacteria with permeability defects," stated the authors. (biobanking.com)
Outer membrane permeability1
Jonathan R. Iredell and colleagues from The Westmead Institute for Medical Research including Westmead Biobank, New South Wales, Australia, investigated the effect of altered porin mediated outer membrane permeability alone and together with engineered β-lactamase enzyme mutations on antibiotic susceptibility of K. pneumoniae . (biobanking.com)
Mutations3
For each antibiotic, resistant mutants from both lifestyles possessed conserved 'driver' mutations, however mechanistic distinctions between lifestyles were identified based on the repertoire of secondary mutations. (uea.ac.uk)
The OmpG porin with both stabilizing mutations exhibited a 95% reduction in gating activity. (ox.ac.uk)
Mutations associated with dysfunction of the porin OprD were found in 888 (87.1%) of the genomes and were correlated with carbapenem resistance, and a machine learning model incorporating hundreds of genetic variations among the chromosomal mechanisms of resistance was able to classify resistant genomes. (cdc.gov)
Antibiotic1
Outer membrane porins in Gram-negative bacteria facilitate antibiotic influx. (imperial.ac.uk)
Deficient2
Moreover, substitution with autophosphorylation-deficient PINK1 fails to rescue pink1 null mutant phenotypes. (sdbonline.org)
Mutants functionally deficient in vivo show no binding to K30 polymer in vitro.Apparently, Wzi is a novel outer-membrane lectin that assists in the formation of the bacterial capsule via direct interaction with capsular polysaccharides. (tcdb.org)
Protein2
dcerk1 mutants show protein hyperacetylation in the unique cellular compartments. (nicotinic-receptor.com)
Proteomic analysis of this mutant identified protein candidates that could be responsible for the reduction in virulence. (encyclopedia.pub)
In conclusion, the results of this study demonstrated that the combination of avibactam at 4µg/ml with ceftaroline exponentially expands its spectrum of activity to cover the highly resistant organisms with multiple β-lactamases and serine carbapenemases commonly present in porin defective clinical isolates of K. pneumoniae . (biobanking.com)
Porin channels with a D118Q mutation, at the root of L3, still showed some, albeit milder, sensitivity to polyamine modulation. (tamu.edu)
Experiments to obtain a mutant in the presence of a second copy of impC containing an active-site mutation, in the presence of porin-like gene of M. smegmatis , or in the absence of inositol 1-phosphate synthase activity, were also unsuccessful. (biomedcentral.com)
Plasmid1
The plasmid may affect porin function or open up another entry pathway. (ucl.ac.uk)
Mutagenesis1
Mutagenesis of cysQ (Rv2131c) was initially unsuccessful, but was possible when a porin-like gene of Mycobacterium smegmatis was expressed, and also by gene switching in the merodiploid strain. (biomedcentral.com)
Carbohydrate3
Stability constants for binding of maltopentaose to the mutant channels were measured using titration experiments with the carbohydrate. (uni-wuerzburg.de)
However, binding was again possible when Y118 was replaced by tryptophane (W). The carbohydrate-induced block of the channel function could also be used for the study of current noise through the different mutant LamB-channels. (uni-wuerzburg.de)
The analysis of the power density spectra of some of the mutants allowed the evaluation of the on- and off-rate constants (k1 and k-1) of carbohydrate binding to the binding-site inside the channels. (uni-wuerzburg.de)
Sensitivity1
The sensitivity to copper ions is due to the uptake of the ions by the Omp F porin. (ucl.ac.uk)
Uptake1
Molecular simulations elucidate the uptake mechanism of kanamycin through these porins. (chemrxiv.org)
Resistance1
One strain had neither porin alteration or can also contribute to cephamycin resistance. (cdc.gov)
Species1
PI-containing molecules have been demonstrated as essential for growth in the fast-growing species Mycobacterium smegmatis , as mutants lacking PI synthase are not viable [ 8 ]. (biomedcentral.com)
Channels1
The gating activity of the wild-type and mutant channels were studied by patch-clamp of liposomes containing reconstituted outer membrane fractions, in the absence or the presence of either polyamine spermine or cadaverine. (tamu.edu)
Antibiotics1
Despite decades of therapeutic application of aminoglycosides, it is still a matter of debate if porins contribute to the translocation of the antibiotics across the bacterial outer membrane. (chemrxiv.org)
Activity3
dcerk1 mutants show acetylation of many OXPHOS subunits and decrease in complex V activity, that is rescued by supplementing NAD+ and inhibited by nicotinamide. (nicotinic-receptor.com)
We examined the porin-forming activity of the FupA/B proteoliposomes using a fluorescent 8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt (ANTS) probe. (encyclopedia.pub)
Bacterial outer membrane porins have a robust beta-barrel structure and therefore show potential for use as stochastic sensors based on single-molecule detection. (ox.ac.uk)
Acid1
Surprisingly, in addition to LOS-sialic acid, we found that gonococcal porin (PorB) mediated binding to multiple Siglecs. (datadryad.org)
Analysis1
Raw data and statistical analysis of binding assay with Siglec-Fcs and gonococcal porins. (datadryad.org)
Channel2
Porin mutants with new channel properties. (expasy.org)
Structural studies have demonstrated that the extracellular L3 loop of porin constricts the channel and suggest that this loop might be involved in channel selectivity and gating. (tamu.edu)
Play1
We assume that the K1 capsule and OmpA porin play a key role in the virulence of E. marmotae. (bvsalud.org)
Shown1
It was shown that both mutant plasmids sensitise 1829 to these two agents. (ucl.ac.uk)
Expression1
Cross-resistant mutants arose through a variety of mechanisms, including constitutive expression of multidrug efflux, hypermutation, cell wall remodelling and porin loss. (uea.ac.uk)
Regions1
Structural and modeling data, for example, identify TprK as an integral outer membrane porin with the V regions exposed on the pathogen's surface. (biorxiv.org)
Type1
This included efflux pump regulators, porins and biofilm-dependent pathways including type IV pili and alginate biosynthesis genes. (uea.ac.uk)
Transport1
Furthermore, LamB mutants were created in which one, two, three, four and five aromatic residues were replaced to study their effects on ion and maltopentaose transport through LamB. (uni-wuerzburg.de)