These abnormalities were less frequent in transgenic zebrafish embryos expressing rat αA-crystallin suggesting a specific role of α-crystallins in embryonic lens development. (nih.gov)
We then analyzed the expression of a number of Class I and Class II Hh responsive genes in wild type, Hh mutant, and Hh over-expressing zebrafish embryos. (zfin.org)
This comprehensive expression study provides an important tool for the characterization of Hh signaling in zebrafish and provides a sensitive assay for determining the degree to which newly identified zebrafish mutants affect Hh signaling. (zfin.org)
Via positional cloning, we show that mutations in a zebrafish LTCC encoding gene, cav1.3a , underlie the auditory-vestibular defects of gemini ( gem ) circler mutants. (jneurosci.org)
RT-PCR was used to show that the beta3 and alpha2 subunit RNAs were present in zebrafish embryos only 2-5hours post-fertilization (hpf) while alpha7 subunit RNA was not detected until 8hpf, supporting the differential regulation of nAChRs during development. (zfin.org)
nAChR binding techniques were used to detect the early expression of two high-affinity [3H]-epibatidine binding sites in 2 days post-fertilization (dpf) zebrafish embryos with IC(50) values of 28.6pM and 29.7nM and in 5dpf embryos with IC(50) values of 28.4pM and 8.9nM. (zfin.org)
Zebrafish embryos offer a unique opportunity to investigate the mechanisms by which nicotine exposure impacts early vertebrate development. (oregonstate.edu)
The nicotine-induced alteration in muscle development does not occur in the zebrafish mutant (sofa potato, [sop]), which lacks muscle-specific AChRs. (oregonstate.edu)
Developmental2
When nonhuman mammalian development is compared with human development, the study subjects must be compared at the same developmental stage (fetal, perinatal, postnatal) When collected appropriately, data from experimental studies of nonhuman mammalian embryos elucidate important aspects of human facial development. (medscape.com)
The investigators also state that the developmental primordia from which the premaxilla of nonmammalian tetrapods arises only rarely is involved in forming the upper jaw in therian mammals, instead developing into a motile nose. (medscape.com)
Genes2
These studies provide genetic evidence for an essential function of a homeodomain protein in ß-catenin-mediated induction of the dorsal gastrula organizer and place boz at the top of a hierarchy of zygotic genes specifying the dorsal midline of a vertebrate embryo. (zfin.org)
In contrast, more dorsally expressed genes were variably affected in different Hh pathway mutants, indicating mid-levels of Hh signaling are differentially affected. (zfin.org)
Dorsal1
For example, the formation of feather tracts on the dorsal surface of chick embryos [ 9 ] and mammary (milk) lines on the ventral surface of embryonic mammals [ 10 ] serve to pre-pattern regions competent for the initiation of these structures. (biomedcentral.com)
Zygotic1
A more consistent and severe lens phenotype was evident in maternal/zygotic αA-crystallin mutants compared to those observed by morpholino knockdown. (nih.gov)
Defects1
Merlin mutants show defects in nuclear migration and mRNA localisation in the oocyte. (ox.ac.uk)
Embryonic1
Shortly after this the embryo begins to undergo embryonic turning, the process that results in the embryo taking up the classic foetal position. (biomedcentral.com)
Occurs1
This occurs in a L-R asymmetric manner such that the caudal-most region of the embryo passes to the right side of the head. (biomedcentral.com)
Functional1
Apical FM1-43 uptake, however, is unaffected in gem mutant HCs, suggesting that mechanotransduction channels are functional. (jneurosci.org)
Expression1
Further, ventral-most Class II gene expression was completely lost in all Hh pathway mutants analyzed, indicating high levels of Hh signaling are blocked in all of these mutants. (zfin.org)
Development3
Overexpression of boz in the extraembryonic yolk syncytial layer of boz mutant embryos is sufficient for normal development of the overlying blastoderm, revealing an involvement of extraembryonic structures in anterior patterning in fish similarly to murine embryos. (zfin.org)
We previously demonstrated that secondary spinal motoneurons in nicotine-exposed embryos were delayed in development and that their axons made pathfinding errors (Svoboda, K.R., Vijayaraghaven, S., Tanguay, R.L., 2002. (oregonstate.edu)
Even though muscle development is unaffected by nicotine exposure in sop mutants, motoneuron axonal pathfinding errors still occur in these mutants, indicating a direct effect of nicotine exposure on nervous system development. (oregonstate.edu)
Cells1
Merlin is also required non-autonomously, only in follicle cells that have received the Gurken signal, to maintain cell polarity and limit proliferation, but is not required in embryos and larvae. (ox.ac.uk)
When nonhuman mammalian development is compared with human development, the study subjects must be compared at the same developmental stage (fetal, perinatal, postnatal) When collected appropriately, data from experimental studies of nonhuman mammalian embryos elucidate important aspects of human facial development. (medscape.com)
The investigators also state that the developmental primordia from which the premaxilla of nonmammalian tetrapods arises only rarely is involved in forming the upper jaw in therian mammals, instead developing into a motile nose. (medscape.com)
Drosophila2
Additionally, Clogmia lost maternal germ plasm, which contributes to embryo polarity in fruit flies ( Drosophila ). (nih.gov)
Their name derives from the observation of a hedgehog-like appearance in DROSOPHILA embryos with genetic mutations that block their action. (lookformedical.com)
Proteins1
These mutant embryos exhibit fragmented or decondensed nuclei and accumulate higher levels of SUMO-conjugated proteins, suggesting a role for Dgrn in genome stability. (washington.edu)