• An alternative strategy for insertional mutagenesis has been used in vertebrate animals to find genes that cause cancer. (wikipedia.org)
  • While the selective inactivation of Ophiostoma genes by insertional mutagenesis remains a challenge, an alternative approach based on RNA interference is now available for down-regulating the expression of targeted genes. (sisef.it)
  • The researchers used a molecular biology technique called gene-trap insertional mutagenesis to identify hundreds of human genes that enable viruses to hijack a human cell, but are not necessary for the cell itself to survive. (infectioncontroltoday.com)
  • The contributors describe strategies for producing genetic models, including transgenic germline models, gene knockouts and knockins, and conditional and inducible systems, as well as models derived using transposon-based insertional mutagenesis, RNA interference, viral-mediated gene delivery, and chemical carcinogens. (cshlpress.com)
  • Eucaryotic elongation factor 2 (EF-2) contains a post-translationally modified histidine residue termed diphthamide that is specifically ADP-ribosylated by diphtheria toxin (DT) or Pseudomonas exotoxin A. To analyze the potential physiological role of ADP-ribosylation of EF-2 by cellular ADP-ribosyl transferase, we constructed DT-resistant, non-ADP-ribosylatable Saccharomyces cerevisiae EF-2 by site-directed mutagenesis and expressed the mutant EF-2 in yeast. (nih.gov)
  • In molecular biology, insertional mutagenesis is the creation of mutations in DNA by the addition of one or more base pairs. (wikipedia.org)
  • Such insertional mutations can occur naturally, mediated by viruses or transposons, or can be artificially created for research purposes in the lab. (wikipedia.org)
  • Historically, lentiviral vectors included strong viral promoters which had a side effect of insertional mutagenesis, nuclear DNA mutations that effect the function of a gene. (wikipedia.org)
  • The CDC defines DNA vaccines as "purified plasmid preparations containing one or more DNA sequences capable of inducing and/or promoting an immune response against a pathogen," yet there is no proof that this really works as stated, and research shows that when these sequences are injected they can cause "insertional mutagenesis," which means gene and cell mutations can result - and that's also the definition of carcinogenesis, or "cancer. (ipetitions.com)
  • Comprehensive Functional Analysis of the Enterococcus faecalis Core Genome Using an Ordered, Sequence-Defined Collection of Insertional Mutations in Strain OG1RF. (cdc.gov)
  • An insertional mutagenesis protocol using Agrobacterium tumefacience-mediated transformation is developed to transform S. sclerotiorum. (usda.gov)
  • Advancing Functional Genetics Through Agrobacterium -Mediated Insertional Mutagenesis and CRISPR/Cas9 in the Commensal and Pathogenic Yeast Malassezia . (bvsalud.org)
  • Insertional mutagenesis is possible whether the virus is of the self-inactivating types commonly used in gene therapy or competent to replicate. (wikipedia.org)
  • Insertional inactivation is a technique used in recombinant DNA engineering where a plasmid (such as pBR322) is used to disable the expression of a gene. (wikipedia.org)
  • A mutant library was created with insertional mutagenesis via electroporation in order to transform a BASTA resistance gene into conidial nuclei. (uni-muenchen.de)
  • Mouse insertional mutagenesis experiments support the designation of JARID2 as a cancer causing gene. (sanger.ac.uk)
  • Reference: Cheng F, Murray JL, Zhao J, Sheng J, Zhao Z, Rubin DH (2016) Systems Biology-Based Investigation of Cellular Antiviral Drug Targets Identified by Gene-Trap Insertional Mutagenesis. (infectioncontroltoday.com)
  • tax causes malignant transformation not through integration and insertional mutagenesis, as many retroviruses do, but by inhibition of DNA repair (base excision pathway) and trans-activating disruption of cellular growth control mechanisms ( 2 ). (cdc.gov)
  • Because many viruses integrate their own genomes into the genomes of their host cells in order to replicate, mutagenesis caused by viral infections is a fairly common occurrence. (wikipedia.org)
  • Not all integrating viruses cause insertional mutagenesis, however. (wikipedia.org)
  • There is an example of an insertional mutagenesis event caused by a retrotransposon in the human genome where it causes Fukuyama-type muscular dystrophy. (wikipedia.org)
  • What you might get is what's known as "insertional mutagenesis" and it can result in all sorts of bad things happening. (civileats.com)
  • Historically, lentiviral vectors included strong viral promoters which had a side effect of insertional mutagenesis, nuclear DNA mutations that effect the function of a gene. (wikipedia.org)
  • However, as Signal Transduction and Targeted Therapy notes, LV vectors have historically carried the risk of insertional mutagenesis, though this risk can be minimized by using third-generation, self-inactivating LV vectors. (corning.com)
  • They also have lower risk of genotoxicity due to insertional mutagenesis than gammaretroviral vectors. (researchsquare.com)
  • Unlike viral vectors, non-viral systems do not carry the risk of immune responses or insertional mutagenesis. (atonce.com)
  • In molecular biology, insertional mutagenesis is the creation of mutations in DNA by the addition of one or more base pairs. (wikipedia.org)
  • Ideally, the insertional mutagenesis system developed would permit a wide range of genetic analyses and manipulations, including enhancer-trapping, conditional knockouts, conditional expression or overexpression, etc. (nih.gov)
  • The contributors describe strategies for producing genetic models, including transgenic germline models, gene knockouts and knockins, and conditional and inducible systems, as well as models derived using transposon-based insertional mutagenesis, RNA interference, viral-mediated gene delivery, and chemical carcinogens. (cshlpress.com)
  • 17. Insertional mutagenesis and development of malignancies induced by integrating gene delivery systems: implications for the design of safer gene-based interventions in patients. (nih.gov)
  • Because many viruses integrate their own genomes into the genomes of their host cells in order to replicate, mutagenesis caused by viral infections is a fairly common occurrence. (wikipedia.org)
  • Until the occurrence of these adverse events was related to an integrating retroviral vector, the risk of insertional mutagenesis was estimated as only 10 −6 -10 −8 per integration event. (medscape.com)
  • Specifically, we characterize the mechanism of vector integration into the host cell genome and the risks of insertional mutagenesis involved in this process. (unc.edu)
  • tax causes malignant transformation not through integration and insertional mutagenesis, as many retroviruses do, but by inhibition of DNA repair (base excision pathway) and trans-activating disruption of cellular growth control mechanisms ( 2 ). (cdc.gov)
  • This RFA, "Tools for Insertional Mutagenesis in the Mouse," is related to one or more of the priority areas. (nih.gov)
  • His group uses MSI to support their studies of insertional mutagenesis for cancer gene discovery and mouse functional genomics. (umn.edu)
  • Mouse insertional mutagenesis experiments DO NOT support the designation of CTU1 as a cancer causing gene. (sanger.ac.uk)
  • This can lead to poor expression of the integrated gene if it is inserted into a region of heterochromatin, or to undesirable insertional mutagenesis of the host cell if it integrates in an important gene. (gla.ac.uk)
  • Here, we describe the occurrence of clonal T cell acute lymphoblastic leukemia (T-ALL) promoted by insertional mutagenesis in a completed gene therapy trial of 10 SCID-X1 patients. (nih.gov)
  • Analysis of the patients with leukemia revealed insertional mutagenesis in the leukemic T-cell clone, which was correlated with the onset of leukemia. (medscape.com)
  • He explains that they are required to follow-up patients for 15 years to monitor for insertional mutagenesis-caused leukemias. (the-scientist.com)