• Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
  • On the other hand, despite the atrophy and reduced myogenic potential of the EDL muscles in old rats, when an EDL muscle is cross-age transplanted from an old donor rat into a young host rat, the muscle regenerates as much mass and has as high a capacity for force development as a young muscle transplanted into a young host [4] . (josam.org)
  • Defective muscle regeneration can severely affect re-innervation by motor axons, and neuromuscular junctions (NMJs) development, ultimately leading to skeletal muscle atrophy. (biomed.news)
  • The effect of deer antler extract on muscle differentiation and muscle atrophy were evaluated to minimize muscle loss following aging. (kosfaj.org)
  • In addition, the expression levels of genes related to muscle differentiation and atrophy were confirmed through qRT-PCR. (kosfaj.org)
  • Gene expression of AMP-activated protein kinase (AMPK), MyoD1, and myogenin, along with the muscle atrophy factors muscle RING finger-1 (MuRF-1) and forkhead box O3a (FoxO3a) upon addition of deer antler extracts to muscle-atrophied C2C12 cells was determined by qRT-PCR after treatment with AICAR. (kosfaj.org)
  • In addition, gene expression of MyoD1 and myogenin in the muscle atrophy cell model was significantly increased compared that into the CON. (kosfaj.org)
  • Therefore, our findings indicate that antler extract can increase the expression of MyoD1, Myf5 and myogenin, inhibit muscle atrophy, and promote muscle differentiation. (kosfaj.org)
  • Muscle atrophy with aging is accompanied by muscle damage caused by oxidative damage and malnutrition caused by lack of muscle metabolism. (kosfaj.org)
  • It is a protein with a key role in regulating muscle differentiation or myogenesis, specifically the development of skeletal muscle. (wikipedia.org)
  • Although it is expressed in both the epaxial (to become muscles of the back) and hypaxial (body wall and limb muscles) portions of the myotome, it is regulated differently in these tissue lines, providing part of their alternative differentiation. (wikipedia.org)
  • The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
  • All cells have been tested for myotube differentiation. (zen-bio.com)
  • Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)
  • Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts. (zen-bio.com)
  • Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. (unipd.it)
  • The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. (biomedcentral.com)
  • IL-2/IL2R also promotes the differentiation of T cells into effector T cells. (ilreceptor.com)
  • Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
  • Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus, Bmc Genomics, 20, 156, https://doi.org/10.1186/s12864-019-5530-7, 2019. (copernicus.org)
  • Although adipocyte differentiation starts early in fetal stages, the increase in adipose cell number and size is maintained along early postnatal growth and later development [ 6 ]. (biomedcentral.com)
  • Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation. (bioseek.eu)
  • Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)
  • The manipulation of fat stores is an obvious therapeutic dream, but disruption of the normal differentiation or development of white adipose tissues (WAT) causes ectopic lipid storage and severe pathology (lipodystrophy) in both humans and experimental animals. (bioseek.eu)
  • Morphological changes according to the effect of antler extracts on muscle differentiation were confirmed by Jenner-Giemsa staining. (kosfaj.org)
  • Therefore, promotion of myoblast proliferation and differentiation and induction of myotube hypertrophy should be beneficial for muscle regeneration and control of muscle mass. (kosfaj.org)
  • Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)
  • ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
  • Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
  • Skeletal myoblasts are available from both male and female donors. (zen-bio.com)
  • Because of the decreased proliferation potential [9] and lower absolute number of satellite cells in muscles from the aged rats compared with adult rats [1,10,11] , one may hypothesize that the aged rats would not yield enough myoblasts to produce myooids in culture. (josam.org)
  • We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)
  • These monocyte myoblasts proliferate and differentiate, following which, they fuse with existing muscle fibers to form multinucleated myotubes and myofibers. (kosfaj.org)
  • Although Myf5 is mainly associated with myogenesis, it is expressed in other tissues, as well. (wikipedia.org)
  • Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
  • To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
  • What was surprising is that administering these inhibitors to the whole animal appears to reduce TGF-beta levels in the whole animal, suggesting some kind of feed-back and perhaps effects on other tissues as well as muscle. (ca.gov)
  • The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
  • The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
  • Skeletal muscle regenerative capacity later diminishes with advancing age and decreasing numbers of SCs, and muscle tissue is steadily replaced by adipose and connective tissues[ 13 ]. (biomedcentral.com)
  • Comparison of transcriptome of skeletal muscles and other tissues between phenotypically different pig breeds has been proposed to improve the understanding of the genetic mechanisms underlying differences in growth and meat quality [ 10 ]. (biomedcentral.com)
  • Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. (u-paris.fr)
  • The epidemic of obesity, closely associated with increases in diabetes, hypertension, hyperlipidemia, cancer and other disorders, has propelled a major interest in adipose cells and tissues. (bioseek.eu)
  • Adipose tissues contain two distinct types of fat cells, white and brown. (bioseek.eu)
  • Promotion of increased BAT development in humans, however, offers the possibility of increasing energy expenditure without necessarily causing dysfunction in other tissues. (bioseek.eu)
  • Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)
  • Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
  • In this study, we present a fully automatic multi-atlas segmentation pipeline we have developed for segmentation of quadriceps muscles from magnetic resonance images obtained in twenty five young healthy males.Materials and Methods: A non-linear registration process based on ANTs algorithm was applied to thigh images initially automatically segmented for bone, muscle and fat tissues. (jain-foundation.org)
  • Here, we describe a platform for engineering and testing highly functional biomimetic muscle tissues with a resident satellite cell niche and capacity for robust myogenesis and self-regeneration in vitro. (duke.edu)
  • We determined the developmental and functional status of both skeletal muscle progenitor cells as well as adult human satellite cells. (ca.gov)
  • A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. (ca.gov)
  • Still, the existence of these separate "MyoD-dependent" and "Myf5-dependent" subpopulations has been disputed, with some claiming that these MRFs are indeed coexpressed in muscle progenitor cells. (wikipedia.org)
  • Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
  • Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
  • The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
  • This work could provide a cell therapy for muscle disorders including muscular dystrophies or sarcopenia. (ca.gov)
  • A large majority of California residents are affected or will be affected with a muscle disease in their lifetime including severe muscular dystrophies, aging related muscle loss called sarcopenia or muscle weakness. (ca.gov)
  • Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
  • Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
  • However, during aging, extensive injury, or pathological conditions, the complete regenerative program is severely affected, with a progressive loss of muscle mass and function, a condition known as sarcopenia. (biomed.news)
  • One of the great advantages of tissue engineering of skeletal muscle is that adult skeletal muscle contains many myogenic precursor stem cells, termed "satellite cells", which are involved in the repair and regeneration of skeletal muscle throughout the lifespan. (josam.org)
  • Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
  • We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
  • Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
  • Cellular and molecular regulation of muscle regeneration. (scielo.br)
  • Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies [med. (emf-portal.org)
  • Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
  • The formation of skeletal muscle:from somite to limb. (scielo.br)
  • 7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)
  • 10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
  • Culture conditions instigate neural lineage arrest and promote fetal hypaxial myogenesis toward limb axial anatomical identity, with generation of sustainable uncommitted PAX7 myogenic progenitors and fibroadipogenic (PDGFRa+) progenitor populations equivalent to those from the second trimester of human gestation. (bvsalud.org)
  • We will perform single cell RNA sequencing (scRNA-seq) of embryonic, fetal, juvenile and adult muscle stem cells (MuSCs) and identify unique gene signatures and cell states across human myogenesis. (ca.gov)
  • Myogenic factor 5 is a protein that in humans is encoded by the MYF5 gene. (wikipedia.org)
  • While the product of this gene is capable of directing cells towards the skeletal muscle lineage, it is not absolutely required for this process. (wikipedia.org)
  • To understand the function of SFRP1 and miRNA-1/206 in swine myogenesis, we first predicted the targets of miRNA-1/206 with the TargetScan and PicTar programs, and analyzed the molecular characterization of the porcine SFRP1 gene. (biomedcentral.com)
  • Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. (biomedcentral.com)
  • Duchenne muscular dystrophy (DMD), the most common and severe form of muscular dystrophy, is caused by mutations in the dystrophin gene, the largest gene identified in the human genome. (biomedcentral.com)
  • Circular transcripts of the testis-determining gene Sry in adult mouse testis, Cell, 73, 1019-1030, https://doi.org/10.1016/0092-8674(93)90279-y, 1993. (copernicus.org)
  • Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)
  • Finally, Prdm16-deficient brown fat displays an abnormal morphology, reduced thermogenic gene expression and elevated expression of muscle-specific genes. (bioseek.eu)
  • This webinar included overviews of the clinical phenotype and genetics of dysferlinopathy, and also highlighted some of the findings of recent clinical studies, in particular the Clinical Outcome Study in Dysferlinopathy (COS), as well as large-scale sequencing studies that document the spectrum of mutations of the DYSF gene and carry implications about the role of the dysferlin protein in muscle biology. (jain-foundation.org)
  • It functions during that time to commit myogenic precursor cells to become skeletal muscle. (wikipedia.org)
  • During embryonic myogenesis, migration of skeletal myogenic progenitors is essential to generate the anlagen of limbs, diaphragm and tongue, whereas in post-natal skeletal muscles, migration of muscle satellite (stem) cells towards regions of injury is necessary for repair and regeneration of muscle fibres. (crick.ac.uk)
  • 1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
  • 6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
  • Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
  • Skeletal muscle myosin heavy chain (MHC) content was measured and MHC isoforms were separated on SDS-PAGE gels and quantified. (josam.org)
  • Adult and aged rat myooids had only ~35-60% of the skeletal muscle MHC content of control skeletal muscle from rats, the remaining MHC content consisting of isoforms found in cultured fibroblasts but not in control skeletal muscle. (josam.org)
  • In addition, myooids expressed only developmental isoforms of skeletal muscle MHC, known to generate less specific force than adult isoforms. (josam.org)
  • Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
  • We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)
  • We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. (nature.com)
  • When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
  • Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
  • In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
  • The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)
  • Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)
  • It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)
  • 3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)
  • We are developing a target product profile for human satellite cells derived from human pluripotent stem cells which will be used to use muscle stem cells in personalized medicine for patients with devastating muscle wasting or degenerative diseases. (ca.gov)
  • This can be achieved using induced pluripotent stem cells (iPSCs), human adult cells that have been 'reprogrammed' to behave like cells in the embryo that can develop in to almost any cell in the body. (bvsalud.org)
  • Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
  • The overall aim is to gain insight into mechanisms of skeletal muscle adaptation in elderly individuals. (regionh.dk)
  • Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)
  • Here, we review mechanisms of myogenic stem/progenitor cell migration during skeletal muscle development and post-natal regeneration. (crick.ac.uk)
  • These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
  • Exploring the mechanisms through which the immune system can modulate skeletal muscle wasting, injury, regeneration and growth is a particular focus of the lab. (ucla.edu)
  • Other NIH-funded investigations in our lab explore epigenetic mechanisms through which an anti-aging protein called Klotho affects myogenesis and muscle regeneration in neonatal and aging muscle. (ucla.edu)
  • ont permis la publication de plus de 370 articles et monographies, dont une 44 en 2020. (u-paris.fr)
  • Myooids were activated by transverse electrical fields in a manner similar to that used to activate whole skeletal muscles in vitro during standard physiological measures of muscle function [8] . (josam.org)
  • in these cells physiological lev els of E2 and low levels of xenoestrogens can rapidly reverse actions of the DAT. (inhibitorkit.com)
  • Skeletal muscle is characterized by a remarkable capacity to rearrange after physiological changes and efficiently regenerate. (biomed.news)
  • In living organisms, if the temperature exceeds the normal range (thermo-neutral zone), it disturbs the normal physiological functioning and induces cell injury. (frontiersin.org)
  • Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury. (duke.edu)
  • Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
  • Here we report that human skeletal muscle organoids can be differentiated from induced pluripotent stem cell lines to contain paraxial mesoderm and neuromesodermal progenitors and develop into organized structures reassembling neural plate border and dermomyotome. (bvsalud.org)
  • Single-cell comparison to human fetal and adult myogenic progenitor /satellite cells reveals distinct molecular signatures for non-dividing myogenic progenitors in activated (CD44High/CD98+/MYOD1+) and dormant (PAX7High/FBN1High/SPRY1High) states. (bvsalud.org)
  • Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
  • Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
  • In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
  • Indeed, several human diseases are characterized by attenuated repair responses and imbalances in the inflammatory response with increased number of infiltrating macrophages [ 3 - 5 ]. (hindawi.com)
  • Alternatively activated M2 macrophages comprise cells exposed to low concentrations of M-CSF in the presence of IL-4, IL-13, or IL-10. (hindawi.com)
  • In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
  • Macrophages later arrive at the tissue to remove cellular debris, and satellite cells (SCs) are activated and proliferate to induce myofiber regeneration. (biomedcentral.com)
  • For example, our findings have shown that macrophages and eosinophils are key effector cells in the pathogenesis of Duchenne muscular dystrophy. (ucla.edu)
  • However, discoveries in our lab have also shown that regulatory interactions between cytotoxic, M1 macrophages in dystrophic muscle and anti-inflammatory, M2 macrophages are important in regulating the balance between the death of dystrophic muscle and regenerative processes. (ucla.edu)
  • The objective of this work was the evaluation of muscle transcriptome profile in piglets of both genetic types, in order to identify genes, pathways and regulatory factors responsible for their phenotypic differences. (biomedcentral.com)
  • One of the key reasons for this negative outcome is the limited migration of donor cells, which hinders the overall cell engraftment potential. (crick.ac.uk)
  • Together, these data suggest that CXCR4 plays an important role in donor cell engraftment. (biomedcentral.com)
  • Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)
  • We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
  • However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
  • We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
  • Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
  • We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
  • Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
  • MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
  • Until quite recently, brown adipose tissue (BAT) was thought to be of metabolic importance only in smaller mammals and infant humans. (bioseek.eu)
  • Some evidence shows that Myf5 and MyoD are responsible for the development of separate muscle lineages, and are not expressed concurrently in the same cell. (wikipedia.org)
  • As no muscle is formed in Myf5;Myod double mutant zebrafish, Myf5 cooperates with Myod to promote myogenesis. (wikipedia.org)
  • All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
  • Evaluate myogenic ability and functional differences of embryonic, fetal, juvenile and adult MuSCs in fusion, proliferation, engraftment and repopulation assays in mouse models of muscle disease. (ca.gov)
  • A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. (nature.com)
  • In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
  • 20% of the original mass invariably results in functional impairment with limited regeneration capacity, which requires reconstructive surgical procedures such as autologous muscle flap transfers 1 . (nature.com)
  • We also demonstrated the feasibility of using these 3D bioprinted human skeletal muscle constructs to treat critical-sized muscle defect injuries with structural and functional restoration in a rodent model 2 . (nature.com)
  • Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
  • Although administration of various myogenic cell types has been done intramuscularly or intravascularly, functional restoration has not been achieved yet in patients with degenerative diseases affecting multiple large muscle. (crick.ac.uk)
  • Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
  • We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
  • The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
  • Duchenne muscular dystrophy (DMD) is the most common muscular dystrophy and an X-linked recessive, progressive muscle wasting disease caused by the absence of a functional dystrophin protein. (biomedcentral.com)
  • Functional three-dimensional skeletal muscle constructs (myooids) were engineered from myogenic cells harvested from the muscles of adult and aged rats. (josam.org)
  • Satellite cells are in general easy to isolate from explanted skeletal muscle, and presumably muscle biopsies from an adult mammal would provide an ample supply of cells committed to the myogenic developmental pathway to facilitate the in vitro engineering of functional skeletal muscle. (josam.org)
  • Prior to tissue engineering of functional skeletal muscle from satellite cells harvested from aged mammals, two key questions must be addressed. (josam.org)
  • First, to what extent are the satellite cells from aged mammals capable of fusion into functional myotubes, and second, do satellite cells from aged mammals have any form of impaired function when compared with those from young or adult mammals? (josam.org)
  • To study the capacity of satellite cells to form into functional skeletal muscle tissue de novo in culture, we have developed several in vitro tissue models. (josam.org)
  • Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo. (duke.edu)
  • Using a mouse dorsal window implantation model and transduction with fluorescent intracellular calcium indicator, GCaMP3, we nondestructively monitored, in real time, vascular integration and the functional state of engineered muscle in vivo. (duke.edu)
  • We will identify unique cell surface markers on in vivo derived MuSCs that distinguish PAX7-positive cells from other muscle cells and across cell states. (ca.gov)
  • These approaches have achieved bioengineered skeletal muscle tissue fabrication with cellular alignment in vitro, and some showed a degree of therapeutic potential in vivo. (nature.com)
  • This is being done in human studies in vivo and in vitro. (regionh.dk)
  • We have chosen to work on human precursors, either in vitro or in vivo, in xenograft models that we have developed in successive European networks. (institut-myologie.org)
  • The described in vitro and in vivo models of biomimetic engineered muscle represent enabling technology for novel studies of skeletal muscle function and regeneration. (duke.edu)
  • More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
  • These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration. (nature.com)
  • These constructs (mm 3 -cm 3 scale) consisted of tens to hundreds of long parallel myofiber bundles, containing densely packed, highly viable, and aligned muscle cells. (nature.com)
  • While host nerve integration and formation of NMJs were evident within the implanted skeletal muscle constructs, we observed that the constructs did not support the full restoration of defected muscles at 8-week post-implantation, which is likely due to the delayed integration of host nerve. (nature.com)
  • Enhancing Myoblast Fusion and Myotube Diameter in Human 3D Skeletal Muscle Constructs by Electromagnetic Stimulation [med. (emf-portal.org)
  • The use of mesenchymal stem cells (MSCs) as a regenerative therapy holds the promise of regrowing injured vasculature, helping DM patients live healthier and longer lives. (thno.org)
  • Neurogenesis-on-Chip: Electric field modulated transdifferentiation of human mesenchymal stem cell and mouse muscle precursor cell coculture [med. (emf-portal.org)
  • Furthermore, Myf5 is a master regulator of muscle development, possessing the ability to induce a muscle phenotype upon its forced expression in fibroblastic cells. (wikipedia.org)
  • The absence of all three of these factors results in a phenotype with no skeletal muscle. (wikipedia.org)
  • LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)
  • This webinar included findings from metabolic studies on dysferlin-deficient muscle fibers, and the impact of dietary interventions on the phenotype of dysferlinopathy. (jain-foundation.org)
  • Cell Metabolism 2013;17(2):210-24. (scielo.br)
  • More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
  • We observed alteration in biological functions related to extracellular matrix function and organization, cellular adhesion, muscle growth, lipid metabolism and proteolysis. (biomedcentral.com)
  • BAT may thus play a much more important role in human metabolism than was previously appreciated. (bioseek.eu)
  • Although the specific roles and dependency of Myf5 in adipogenesis and neurogenesis have remained to be explored, these findings show that Myf5 may play roles outside of myogenesis. (wikipedia.org)
  • These findings had been corroborated by westernblot analysis displaying a powerful reduction of DNMT1 and DNMT3a protein in each cell lines but not of DNMT3b. (ilreceptor.com)
  • Panobinostat therapy significantly repressed mRNA for DNMT1 and DNMT3a in the two cell lines while no modifications had been observed in DNMT3b levels. (thrombininhibitors.com)
  • Panobinostat treatment drastically repressed mRNA for DNMT1 and DNMT3a in the two cell lines whilst no adjustments have been observed in DNMT3b levels. (ilreceptor.com)
  • In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
  • Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
  • As one of the key cell markers of satellite cells (the stem cell pool for skeletal muscles), it plays an important role in the regeneration of adult muscle. (wikipedia.org)
  • Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
  • In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)
  • Most notably, while Myf5 is activated by Sonic hedgehog in the epaxial lineage, it is instead directly activated by the transcription factor Pax3 in hypaxial cells. (wikipedia.org)
  • Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
  • Molecular biology of the cell. (kcl.ac.uk)
  • Therefore, we aimed to exploit the tumor biological implication and the therapeutic vulnerabilities of a clinically relevant molecular PDAC subgroup characterized by SMAD4 deficiency and high expression of the Nuclear Factor of Activated T-cells (SMAD4-/-/NFATc1High). (bvsalud.org)
  • Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
  • These changes take place on the molecular level impacting properties of specific the different parts of cell inner structure, but resulting in alterations in mechanical properties of the complete cell ultimately. (globaltechbiz.com)
  • Molecular cell. (illinois.edu)
  • Validation of a novel tailored therapeutic option was conducted in patient-derived organoids and cells and transgenic as well as orthotopic PDAC models. (bvsalud.org)
  • Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
  • Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
  • Directional cell migration is a critical process underlying morphogenesis and post-natal tissue regeneration. (crick.ac.uk)
  • While all vertebrates are capable of some types of tissue regeneration, most, including humans, have lost the ability to regenerate whole structures such as limbs ( epimorphic regeneration ), [ 1 ]. (biomedcentral.com)
  • We have a strong expertise on human cellular models (this includes the immortalization facility that we have initiated) - and xenotransplantation (this includes several immunodeficient mouse models and grafting procedures). (institut-myologie.org)
  • Cytoskeleton, U-93631 which is a network of interconnected filaments of different types, connects the cell membrane with underlying sub-cellular components. (globaltechbiz.com)
  • The nucleus is the largest organelle among sub-cellular components, demonstrating solid-elastic behavior [4], and it is typically stiffer than the cell itself [5]. (globaltechbiz.com)
  • We hypothesize that the remaining force deficit is due to cellular and sub-cellular disorganization-Myooids lack the density and organization of sarcomeric arrays seen in skeletal muscle. (josam.org)
  • Our approach provides a robust 3D in vitro developmental system for investigating muscle tissue morphogenesis and homeostasis. (bvsalud.org)
  • Dystrophin has a structural role as a cytoskeletal stabilization protein and protects cells against contraction-induced damage. (biomedcentral.com)
  • Right here, only a transient decrease in protein levels was observed right after 24 to 48 h in both cell lines. (ilreceptor.com)
  • however, priority needs to be given to resistance exercise training, and secondarily to adequate protein intake should be part of this dietary regime to mitigate losing muscle mass. (biomed.news)
  • The poultry sector is noted to make a considerable contribution to global nutrition and food security, which helps in the provision of cheap protein, essential micronutrients, and energy to humans ( 1 ). (frontiersin.org)
  • Macrophage activation has been described as a dynamic process: the same cell may initially induce proinflammatory and cytotoxic reactions and later may take part in the resolution of inflammation and wound healing [ 9 ]. (hindawi.com)
  • Ongoing investigations in the lab are revealing the identity of specific molecules released by myeloid cells that promote muscular dystrophy. (ucla.edu)
  • Metabolic changes in skeletal muscle are a hallmark of several types of muscular dystrophy. (jain-foundation.org)
  • The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)
  • Meat science and muscle biology symposium: stem cell niche and postnatal muscle growth, J. Anim. (copernicus.org)
  • Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. (scielo.br)
  • When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)
  • Models of injury and overload are employed to study the activity of muscle stem cells (satellite cells) and muscle extracellular matrix. (regionh.dk)
  • In addition to this, we are currently studying cross-talk between the muscle and its extracellular matrix, throughout the muscle as well as specifically at the myotendinous junction, in human models of regeneration and overload. (regionh.dk)
  • In addition, cytokines released in the process of myonecrosis recruit inflammatory cells, which release inflammatory cytokines to activate fibroblasts that lay down extracellular matrix proteins and lead to fibrosis[ 12 ]. (biomedcentral.com)
  • Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)
  • They have proinflammatory functions: they produce effector molecules (reactive oxygen and nitrogen intermediates) and inflammatory cytokines (IL-1 β , TNF α , and IL-6) and participate as inducer and effector cells in polarized Th1 responses. (hindawi.com)
  • Firstly, such modeling methods give an insight into how cell parts function under stress. (globaltechbiz.com)
  • In the next reporting period we will re-confirm that the levels of candidate proteins from the 500 antibody array actually are very highly produced by hESCs and that the signals from these proteins are perceived by regenerating muscle cells. (ca.gov)
  • The cell membrane is really a viscous fluid-like matter which includes several lipids, cholesterol, and inserted proteins. (globaltechbiz.com)
  • In particular, in skeletal muscle, specific semaphorin molecules are involved in the restoration and remodeling of the nerve-muscle connections, thus emphasizing their plausible role to ensure the success of muscle regeneration. (biomed.news)