Muscle satellite cell proliferation and differentiation satellite cells in human skeletal muscle. Medical search. Frequent questions

Satellite cells and skeletal muscle regeneration, Compr. (copernicus.org)
In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
20% of the original mass invariably results in functional impairment with limited regeneration capacity, which requires reconstructive surgical procedures such as autologous muscle flap transfers 1 . (nature.com)
Therefore, medications that can prevent the progression of muscle atrophy or promote muscle regeneration are in high demand. (biomedcentral.com)
Considering that reinnervation of the TA muscle can occur even after UVFP through these anastomoses, it would be ideal if drugs that promote nerve regeneration could be used to treat UVFP. (biomedcentral.com)
Satellite cells (SCs), which are precursors of skeletal muscles, such as the TA muscle, play a key role in muscle homeostasis and regeneration. (biomedcentral.com)
SCs are normally in a quiescent state in adult muscles, and start proliferating during muscle regeneration in response to denervation. (biomedcentral.com)
Cellular and molecular regulation of muscle regeneration. (scielo.br)
Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
Conditions involving muscles wasting such as for example muscular dystrophies cachexia and sarcopenia would reap the benefits of strategies URMC-099 that promote skeletal muscles regeneration. (biotech-angels.com)
In particular, her work has been focusing on the molecular mechanisms involved in the modulation of the peripheral synaptic transmission, skeletal muscle adaptation and regeneration in physiological and pathological conditions. (units.it)
The research activity of the group is focused on the identification of new strategies to improve skeletal muscle regeneration. (units.it)
Additionally, we are interested in mechanisms responsible for skeletal muscle recovery and regeneration after injury. (uncg.edu)
More specifically, my laboratory investigates the role inflammation plays in muscle atrophy, as well as during recovery and regeneration after injury. (uncg.edu)
Aged skeletal muscle displays increased fibrosis and impaired regeneration. (uncg.edu)
In aged skeletal muscle, impairments in regrowth and regeneration may be explained by a decreased responsiveness of muscle precursor cells (MPCs) to environmental cues such as growth factors. (uncg.edu)
Skeletal muscle growth and regeneration involves the activity of resident adult stem cells, namely satellite cells (SC). (sdbonline.org)
Collectively, this study provides the first evidence that transient levels of the evolutionarily conserved factor RACK1 are critical for adult SC activation and proper skeletal muscle regeneration, favoring the efficient progression of SC from a committed to a fully differentiated state. (sdbonline.org)
Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
One of the great advantages of tissue engineering of skeletal muscle is that adult skeletal muscle contains many myogenic precursor stem cells, termed "satellite cells", which are involved in the repair and regeneration of skeletal muscle throughout the lifespan. (josam.org)

There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
Acupuncture is effective for the alleviation of denervated skeletal muscle atrophy. (acupunctureofiowa.com)
8] They found that the combined therapy successfully alleviated quadricep atrophy while improving knee function in human subjects. (acupunctureofiowa.com)
The results of the aforementioned research indicates that acupuncture is an effective treatment modality for skeletal muscle atrophy. (acupunctureofiowa.com)
Effect of electroacupuncture on denervated skeletal muscle atrophy and expression of IGF-1/PI3K/AKT in rats [J]. Chinese Acupuncture and Moxibustion, 2018,38(12):1311-1317. (acupunctureofiowa.com)
Experimental study of rat denervated skeletal muscle atrophy [J]. Journal of Shanghai Medical University, 1999, 26(4): 265-268. (acupunctureofiowa.com)
This assumption long seemed valid, with many researchers reporting the presence of disintegrating nuclei in muscle tissue during atrophy induced by inactivity, injury or paralysis. (robert-gorter.info)
Muscles get damaged during extreme exercise, and often have to weather changes in food availability and other environmental factors that lead to atrophy. (robert-gorter.info)
We previously reported that local administration of bFGF 1 month after recurrent laryngeal nerve (RLN) paralysis compensated for atrophy of the thyroarytenoid muscle. (biomedcentral.com)
As increased neuromuscular junctions are expected to maintain myofiber volume, bFGF administration may prevent thyroarytenoid muscle atrophy in the mid to long term. (biomedcentral.com)
These treatments provide only static adjustment to the larynx, and their effects may be limited by the remaining hoarseness due to progressive atrophy of the paralyzed thyroarytenoid (TA) muscle. (biomedcentral.com)
Since myostatin and follistatin bind to the same receptor, the follistatin to myostatin (F:M) ratio is associated with muscle atrophy or hypertrophy [ 12 ]. (biomedcentral.com)
In lung diseases, muscle mass usually decreases and severe atrophy occurs. (jeoct.com)
Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
On the other hand, despite the atrophy and reduced myogenic potential of the EDL muscles in old rats, when an EDL muscle is cross-age transplanted from an old donor rat into a young host rat, the muscle regenerates as much mass and has as high a capacity for force development as a young muscle transplanted into a young host [4] . (josam.org)
Physical activity is known to prevent muscle atrophy, improve or boost metabolic activity, improve respiratory function, and positively impact muscles regarding strength, endurance, and stability. (apeironzoh.com)

Striated muscle tissue is a muscle tissue that features repeating functional units called sarcomeres. (wikipedia.org)
The presence of sarcomeres manifests as a series of bands visible along the muscle fibers, which is responsible for the striated appearance observed in microscopic images of this tissue. (wikipedia.org)
There are two types of striated muscle: Cardiac muscle (heart muscle) Skeletal muscle (muscle attached to the skeleton) Striated muscle tissue contains T-tubules which enables the release of calcium ions from the sarcoplasmic reticulum. (wikipedia.org)
Unlike skeletal and cardiac muscle tissue, smooth muscle tissue is not striated since there are no sarcomeres present. (wikipedia.org)
Striated muscle tissue has more mitochondria than smooth muscle. (wikipedia.org)
The main function of striated muscle tissue is to create force and contract. (wikipedia.org)
Contractions in cardiac muscle tissue are due to a myogenic response of the heart's pacemaker cells. (wikipedia.org)
Adult humans cannot regenerate cardiac muscle tissue after an injury, which can lead to scarring and thus heart failure. (wikipedia.org)
Other vertebrates can regenerate cardiac muscle tissue throughout their entire life span. (wikipedia.org)
Skeletal muscle is able to regenerate far better than cardiac muscle due to satellite cells, which are dormant in all healthy skeletal muscle tissue. (wikipedia.org)
Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
Skeletal muscle is the largest tissue in the body. (frontiersin.org)
More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
These approaches have achieved bioengineered skeletal muscle tissue fabrication with cellular alignment in vitro, and some showed a degree of therapeutic potential in vivo. (nature.com)
It's actually a special type of tissue in your body, where cells are fused together extra close - so close, that they behave a like a giant single cell. (robert-gorter.info)
Skeletal muscle is a target tissue for anabolic steroids. (frontiersin.org)
Adult stem cells are tissue specific and have limited capacity to be expanded while pluripotent stem cells have the ability to differentiate into any cell type of the body while possessing unlimited self-renewal. (biotech-angels.com)
34] Upon damage satellite television cells become activated providing rise to proliferating myoblasts which in turn fuse to existing muscle tissue fibers or even to additional myoblasts to URMC-099 create new myofibers to correct muscle tissue harm. (biotech-angels.com)
To achieve this we firstly established a mouse BAT enriched-miRNA profile by comparing miRNAs expressed in mouse BAT, white adipose tissue and skeletal muscle. (biomedcentral.com)
Discovery of active human brown adipose tissue (BAT) has renewed interest in the possibility that it may regulate metabolism and EE in humans [ 3 - 5 ]. (biomedcentral.com)
She has been reviewer for international Journals ( FASEB J , Cell & Tissue Res , Toxicon, Exp Cell Res ), for national grant agencies (MIUR) and international Foundations (AFM, Association Francaise contre les Myopathies). (units.it)
With ageing, skeletal muscle undergoes a severe reduction in tissue mass, leading to a decrease in strength (sarcopenia). (units.it)
In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)
MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
MSCs can be isolated from a range of tissues, but the most commonly cited sources are bone marrow (BM), adipose tissue, muscle, bone, and perinatal tissues (e.g. (biomedcentral.com)
A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
The results support the concept that fuel excess can drive obesity and diabetes via hyperinsulinaemia, and that an islet beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion. (omicsdi.org)
In testicular tissue, immature Sertoli cell proliferation ability determines the size of mature Sertoli cell populations, which further regulates the spermatogenesis in the adult male animals. (chinagene.cn)
Exosomes are membrane-bound nanovesicles with diameters of 30-150 nm that contain multiple proteins, nucleic acid, lipids and other molecules in a tissue- and cell-specific manner. (biu.ac.il)
The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
Some steroids are more tissue selective, some will antagonize SHBG and/or Estrogen more than others, some will result in a dryer cosmetic look that differs from another, but when it comes to lean muscle growth, they all more or less do the same thing in a nitrogen retention context. (moreplatesmoredates.com)
h and i) PDGFRknock-in mice displayed connective tissue hyperplasia and developed systemic fibrosis including the skeletal muscle,35 and stimulation of PDGFRsignaling promoted proliferation of mouse PDGFRsignaling on human muscle-derived PDGFRon human PDGFRsignaling on the proliferation of PDGFRstimulation promoted the proliferation of PDGFRpromotes the proliferation of PDGFRat the concentrations used in these experiments (see Supplementary Figure S4). (phytid.org)
It is a naturally occurring variant of IGF-1 that has been found in the human brain, cow colostrum, and pig uterine tissue. (purerawz.co)
It is ten times more effective than IGF-1 in the hypertrophy and proliferation of so-called satellite cells in muscle tissue and other tissues due to the changed structure. (purerawz.co)
Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)
Prior to tissue engineering of functional skeletal muscle from satellite cells harvested from aged mammals, two key questions must be addressed. (josam.org)
To study the capacity of satellite cells to form into functional skeletal muscle tissue de novo in culture, we have developed several in vitro tissue models. (josam.org)
The purpose of the current study was to compare the excitability and contractility of myooids engineered from skeletal muscles of 32-month-old rats with that of myooids engineered from adult rat tissue. (josam.org)

Their fate is regulated by the expression of various transcription factors, including paired box 7 (Pax7) and myogenic differentiation (MyoD) [ 6 ]. (biomedcentral.com)
The effects of the isolated EVs on human satellite cell proliferation and differentiation and macrophage activation were examined.Results Exosomes from IM patients decreased human satellite cell proliferation (51%, P<0.01) and inhibited their myogenic differentiation as indicated by lower fusion index (24% inhibition, P<0.01) and expression of myosin heavy chain (72% inhibition, P<0.001). (biu.ac.il)
Another significant difference was in the expression of signaling molecules: Thus, exosomes from BMD patients increased the phosphorylation of Erk and p38, whereas a smaller effect was induced by IM exosomes.Conclusion Exosomes from IM patients decrease satellite cell proliferation and myogenic differentiation compared to healthy exosomes. (biu.ac.il)

Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. (nature.com)
To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
Skeletal muscle progenitors (satellite cells), murine cell line and immortalized human myoblasts are studied in vitro to identify, in a controlled environment, the pathways regulating the regenerative potential of the skeletal muscle. (units.it)
In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)
We have chosen to work on human precursors, either in vitro or in vivo, in xenograft models that we have developed in successive European networks. (institut-myologie.org)
Satellite cells are in general easy to isolate from explanted skeletal muscle, and presumably muscle biopsies from an adult mammal would provide an ample supply of cells committed to the myogenic developmental pathway to facilitate the in vitro engineering of functional skeletal muscle. (josam.org)
Myooids were activated by transverse electrical fields in a manner similar to that used to activate whole skeletal muscles in vitro during standard physiological measures of muscle function [8] . (josam.org)

MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)
We have developed a comprehensive profile of human myogenesis using single cell sequencing. (ca.gov)
We will perform single cell RNA sequencing (scRNA-seq) of embryonic, fetal, juvenile and adult muscle stem cells (MuSCs) and identify unique gene signatures and cell states across human myogenesis. (ca.gov)
In more detail, the team is studying the role of electrical activity and local trophic factors in the microenvironment of satellite cell niche and in the post-mitotic myogenesis. (units.it)

They then leave the cell cycle to self-renew or differentiate as myoblasts. (wikipedia.org)
C2C12 cells, primary myoblasts and myotubes. (sun.ac.za)
After several rounds of proliferation, most SCs differentiate into myoblasts, which fuse to form new myofibers [ 5 ]. (biomedcentral.com)
We previously reported that local injection of single, high-dose bFGF into the paralyzed vocal fold 1 month after RLN transection compensated for an atrophied TA muscle by inducing activation of SC proliferation and increasing the number of myoblasts in the RLN paralysis model of rats [ 12 ]. (biomedcentral.com)
3 20 21 Major factors underlining this poor outcome included low ability of myoblasts to migrate beyond the injection site[22 23 and poor survival of injected cells. (biotech-angels.com)
26-29] As an alternative to poorly-engrafting myoblasts much recent interest has developed around the idea of therapy with stem cells. (biotech-angels.com)
Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
human pluripotent stem cell (hPSC)-derived myoblasts may be cultured on cultureware coated with Corning® Matrigel® hESC-Qualified Matrix. (stemcell.com)
The supplement is combined with a basal medium to prepare MyoCult™-SF Expansion Medium for the culture of primary human and hPSC-derived myoblasts. (stemcell.com)
Myoblasts cultured using MyoCult™-SF Expansion Medium can be differentiated into multinucleated myotubes using MyoCult™ Differentiation Kit (Human) . (stemcell.com)
Because of the decreased proliferation potential [9] and lower absolute number of satellite cells in muscles from the aged rats compared with adult rats [1,10,11] , one may hypothesize that the aged rats would not yield enough myoblasts to produce myooids in culture. (josam.org)

ATGL in beta cells regulates insulin secretion via the production of signalling MAGs. (omicsdi.org)

There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)
Meat science and muscle biology symposium: stem cell niche and postnatal muscle growth, J. Anim. (copernicus.org)
Muscle growth is accompanied by the addition of new nuclei from stem cells to help meet the enhanced synthetic demands of larger muscle cells," explains Schwartz. (robert-gorter.info)
During adolescence muscle growth is enhanced by hormones, nutrition and a robust pool of stem cells, making it an ideal period for individuals to "bank" myonuclei that could be drawn upon to remain active in old age. (robert-gorter.info)
When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
Cell Stem Cell 2013;13(5):590-601. (scielo.br)
Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. (unipd.it)
We determined the true SC state needed to repopulate after reinjury- the gold standard requirement for maintaining stem cell function long term. (ca.gov)
We are developing a target product profile for human satellite cells derived from human pluripotent stem cells which will be used to use muscle stem cells in personalized medicine for patients with devastating muscle wasting or degenerative diseases. (ca.gov)
We will molecularly and functionally define muscle stem cells in human muscle in development, juvenile and adult and develop strategies to generate the most regenerative muscle stem cells from hPSCs. (ca.gov)
The research in this propoal will benefit CA residents tremendously by understanding how to generate a muscle stem cell capable of responding to injury and undergoing long term repopulation after injury to generate new muscle. (ca.gov)
A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. (ca.gov)
These cells have the ability to self-renew and to differentiate into specialized cell types and can be primarily classified as adult and pluripotent stem cells which differ significantly in regard to their differentiation potential and expansion capability. (biotech-angels.com)
Below we review the literature on some of the most studied stem cell populations that have been ascribed with muscle regenerative potential pointing out their advantages as well as caveats. (biotech-angels.com)
1 Adult Stem Cells 1.1 Satellite Cells Studies in the last decade have clearly proven that the regenerative ability of adult skeletal muscle is due to the satellite cell a quiescent stem cell population of muscle precursors located between the basal lamina and sarcolemma of each myofiber. (biotech-angels.com)
31] Down the road Sacco and colleagues proven that intra-muscular transplantation of an individual luciferase-expressing muscle stem cell isolated from Myf5 reporter mice led to intensive proliferation and contribution to muscle materials. (biotech-angels.com)
Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)
From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
He is a member of the Stem Cell Network Centre of Excellence (past member of the Stem Cell Policy Committee and Research Management Committee and current Sub-chair of the Training and Education Committee), and a member of the AllerGen Network Centre of Excellence (Research Management Committee and Co-Chair of the Biomarkers Program). (ubc.ca)
Serra M, Brito C and Alves PM. "Bioengineering strategies for stem cell expansion and differentiation. (sciepub.com)
The use of mesenchymal stem cells (MSCs) as a regenerative therapy holds the promise of regrowing injured vasculature, helping DM patients live healthier and longer lives. (thno.org)

Increase inflammation promotes apoptosis in the epithelial cells, endothelial cells, and airways, that resulting Emphysema. (jeoct.com)
Liver kinase B1 (LKB1) plays pivotal roles in cellular processes such as apoptosis, metabolism, and cell cycle regulation. (omicsdi.org)
Studies have demonstrated that microRNAs (miRNAs) participate in the regulation of the immature Sertoli cell proliferation and apoptosis, but the functions of most identified miRNAs remain unclear. (chinagene.cn)
In this study, based on previous RNA-seq results, we analyzed the regulatory role (s) of miR-362 in proliferation and apoptosis of porcine immature Sertoli cells. (chinagene.cn)
Annexin V-FITC/PI staining assays and qRT-PCR technology were used to test the apoptosis and the expression levels of cell survival-related genes, respectively. (chinagene.cn)
Over-expression of miR-362 and knockdown of ZNF644 arrested the porcine immature Sertoli cells in G 1 and G 2 phases of the cell cycle, respectively, and inhibited proliferation, enhanced apoptosis in the porcine immature Sertoli cells, and significantly regulated the expression levels of cell survival-related genes. (chinagene.cn)
Taken together, these data indicate that miR-362 inhibits proliferation and promotes apoptosis in porcine immature Sertoli cells by targeting the ZNF644 gene, thereby providing the scientific basis for further study on the function(s) of miR-362 in the porcine spermatogenesis. (chinagene.cn)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)

The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells. (biomedcentral.com)
The expression levels of miR-362 and ZNF644 gene were measured using qRT-PCR assay in developing porcine testicular tissues and in immature Sertoli cells transfected with either miR-362 mimic or miR-362 inhibitor. (chinagene.cn)
The ZNF644 gene expression level was significantly regulated by miR-362, demonstrating that miR-362 targets ZNF644 gene and inhibits its expression in porcine immature Sertoli cells. (chinagene.cn)
IGF-I DES belongs to the insulin gene family and has been isolated from the bovine colostrum, human brain, and porcine uterus. (purerawz.co)

Sarcopenia (loss of skeletal muscle mass associated with aging) Polymyositis (chronic inflammation) Dermatomyositis (chronic inflammation with skin rash) Inclusion body myositis (common age-related inflammatory disease) Coronary artery disease (narrowed coronary arteries) Arrhythmia (irregular heartbeat) Cardiomyopathy (disease of the heart muscle) Costamere Shadrin, I. Y. (wikipedia.org)
Sarcopenia, the age-related loss of skeletal muscle mass and function, is becoming more prevalent as the lifespan continues to increase in most populations. (frontiersin.org)
The term sarcopenia was first proposed in 1989 by Irwin Rosenberg to describe a multifactorial syndrome that occurs with age and results in loss of skeletal muscle mass and function ( 3 , 4 ). (frontiersin.org)
My research focuses on investigating the mechanisms responsible for sarcopenia (the age-related loss of skeletal muscle mass and strength) and developing exercise interventions for the treatment/prevention of sarcopenia. (uncg.edu)

Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
1999). such as platelets, macrophages, fibroblasts, epithelial The effect of anabolic steroids appears due to an cells, and endothelial cells. (sagepub.com)

Macrophage activation has been described as a dynamic process: the same cell may initially induce proinflammatory and cytotoxic reactions and later may take part in the resolution of inflammation and wound healing [ 9 ]. (hindawi.com)
The present study showed that in addition to Irisin, semaphorin-3A induced by muscle contraction, prevents inflammation and lung damage caused by COPD or other lung disease. (jeoct.com)
Association of plasma visfatin levels with inflammation, atherosclerosis and acute coronary syndromes (ACS) in humans. (jeoct.com)
The IAL aims to synergise the expertise in muscle and neuromuscular diseases of French teams and the expertise in immunology and inflammation of the Brazilian teams . (institut-myologie.org)
Anabolic and progress smoothly through four distinct phases: steroids exert their effect when bound to androgen hemostasis, inflammation, proliferation, and remodel- receptors with activation of deoxyribonucleic acid ing (Kumara Swamy et al. (sagepub.com)

The perimysium organizes the muscle fibers, which are encased in collagen and endomysium, into fascicles. (wikipedia.org)
Because of the gap junctions, the pacemaker cells transfer the depolarization to other cardiac muscle fibers, in order to contract in unison. (wikipedia.org)
Signals from motor neurons cause skeletal muscle fibers to depolarize and therefore release calcium ions from the sarcoplasmic reticulum. (wikipedia.org)
This process begins with the necrosis of damaged muscle fibers, which in turn induces the inflammatory response. (wikipedia.org)
Satellite cells help to repair and regenerate muscle fibers. (acupunctureofiowa.com)
Until recently, scientists thought this meant that nuclei - the cell control centers that build and maintain muscle fibers - are also lost to sloth. (robert-gorter.info)
Two independent studies - one in rodents and the other in insects - have demonstrated that nuclei are not lost from atrophying muscle fibers, and even remain after muscle death has been initiated. (robert-gorter.info)
30 31 One group took the approach of transplanting sole muscle fibers which demonstrated that every myofiber including 7 or fewer satellite television cells could create over 100 new myofibers in engrafted muscles. (biotech-angels.com)
RACK1 was expressed transiently in the skeletal muscle of post-natal mice, being abundant in the early phase of muscle growth and almost disappearing in adult mature fibers. (sdbonline.org)
After acute injury in muscle of both mouse and the fruit fly Drosophila melanogaster (used as alternative in vivo model) this study found that RACK1 accumulated in regenerating fibers while it declined with the progression of repair process. (sdbonline.org)

Findings also indicated that acupuncture increased the number of progenitor and satellite cells. (acupunctureofiowa.com)
Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
We determined the developmental and functional status of both skeletal muscle progenitor cells as well as adult human satellite cells. (ca.gov)
For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)

The purpose of this brief review is to discuss whether or not ambulatory exercise elicits leg muscle hypertrophy in older adults. (researchgate.net)
In addition, slow-walk training with a combination of leg muscle blood flow restriction elicits muscle hypertrophy only in the blood flow restricted leg muscles. (researchgate.net)
Competitive marathon running and regular high intensity distance running in young and middle-aged adults may not produce leg muscle hypertrophy due to insufficient recovery from the damaging running bout, although there have been no studies that have investigated the effects of running on leg muscle morphology in older subjects. (researchgate.net)
It is clear that skeletal muscle hypertrophy can occur independently of exercise mode and load. (researchgate.net)
High levels are associated with muscle hypertrophy, whereas low levels are epidemiologically associated with metabolic syndrome and diabetes, which negatively impact muscle functions. (frontiersin.org)
This study aimed to determine the efficacy of functional training with and without blood flow restriction (BFR) on muscle hypertrophy indices and strength in older men. (biomedcentral.com)
The findings showed that FT was effective in increasing circulating biomarkers involved in hypertrophy in older adults while adding BFR to FT had a slight increase in these biomarkers but had a tremendous increase in muscle strength. (biomedcentral.com)
Myokines are peptides or proteins released from skeletal muscle and are involved in multiple physiological processes, including metabolism and hypertrophy in the autocrine and paracrine manner [ 6 ]. (biomedcentral.com)
IGF-1 DES is approximately ten times more potent than IGF-I in stimulating hypertrophy and proliferation of cultured cells. (purerawz.co)
It is ten times more potent than IGF-1 in stimulating cell hypertrophy and proliferation because it is unaffected by IGF-1 binding proteins and thus, more bioavailable. (purerawz.co)

Yamashita D, Machigashira M, Miyamoto M, Takeuchi H, Noguchi K, Izumi Y, Ban S. "Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia. (sciepub.com)
Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. (sciepub.com)
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)

As well, many older individuals suffer from sarcopenia, a protracted muscle wasting disorder that typically begins after the age of 50 and involves a loss of about 1% of muscle mass per year ( Woo, 2017 ). (frontiersin.org)
Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
Testosterone concentrations decline as age increase, suggesting that low plasma testosterone levels can cause or accelerate muscle- and age-related diseases, as sarcopenia. (frontiersin.org)
Currently, there is increasing interest on the anabolic properties of testosterone for therapeutic use in muscle diseases including sarcopenia. (frontiersin.org)
This age-related decline in skeletal muscle mass and strength generation, the primary function of skeletal muscle mass, is known as sarcopenia ( 2 , 3 ). (frontiersin.org)
Indeed, most of the intrinsic as well as extrinsic (systemic) muscle changes that occur with age are believed to be involved in the development of sarcopenia ( 5 , 6 ). (frontiersin.org)
This work could provide a cell therapy for muscle disorders including muscular dystrophies or sarcopenia. (ca.gov)
A large majority of California residents are affected or will be affected with a muscle disease in their lifetime including severe muscular dystrophies, aging related muscle loss called sarcopenia or muscle weakness. (ca.gov)

After adipogenic differentiation, PDGFR(data not shown). (phytid.org)

The dynamics of RACK1 levels in isolated adult SC of mice, i.e., progressively high during differentiation and low compared to proliferating conditions, and RACK1 silencing indicated that RACK1 promotes both the formation of myotubes and the accretion of nascent myotubes. (sdbonline.org)
In this study, we examined whether AdipoR mRNA expression in human myotubes correlates with in vivo measures of insulin sensitivity. (diabetesjournals.org)
First, to what extent are the satellite cells from aged mammals capable of fusion into functional myotubes, and second, do satellite cells from aged mammals have any form of impaired function when compared with those from young or adult mammals? (josam.org)

The main interest of her research activity is the nerve-muscle communication and the skeletal muscle physiology, including muscle plasticity and impact of ageing on the neuromuscular system. (units.it)
The results of the proposed research will advance the understanding of important aspects of the neuromuscular physiology such as the modulation of the neuromuscular transmission and the skeletal muscle plasticity. (units.it)

The objective of this study was to elucidate the effects of early bFGF administration on the thyroarytenoid muscle after RLN transection and to investigate the underlying mechanisms. (biomedcentral.com)
Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
However, the pathophysiological mechanisms underlying this muscle syndrome and its relationship with plasma level of androgens are not completely understood. (frontiersin.org)
Comparing and contrasting the molecular mechanisms controlling BAT growth and development in mice and humans will increase our understanding or how human BAT is developed and may identify potential therapeutic targets to increase EE. (biomedcentral.com)
MicroRNAs are molecular mechanisms involved in mouse BAT development however, little is known about the miRNA profile in human BAT. (biomedcentral.com)
Understanding the mechanisms controling BAT differentiation may identify potential therapeutic targets to enhance BAT-controlled metabolism and to increase EE. (biomedcentral.com)
However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
then we review the studies on the molecular mechanisms of LPLI-induced proliferation since January 1999, which will serve as a reference for the researchers in this field. (biomedcentral.com)
These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)

Studies targeted at establishing such effects at cellular level and their correlations with in vivo models, will broaden our understanding of the roles played by androgens on skeletal muscle function in elderly. (frontiersin.org)
Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
Activation of the IGF-1R signaling pathway plays a crucial role in various cellular processes, including cell growth, proliferation, and differentiation. (purerawz.co)
Musgrove E, Seaman M, Hedley D. "Relationship between cytoplasmic pH and proliferation during exponential growth and cellular quiescence. (sciepub.com)
We have a strong expertise on human cellular models (this includes the immortalization facility that we have initiated) - and xenotransplantation (this includes several immunodeficient mouse models and grafting procedures). (institut-myologie.org)
We hypothesize that the remaining force deficit is due to cellular and sub-cellular disorganization-Myooids lack the density and organization of sarcomeric arrays seen in skeletal muscle. (josam.org)
Mostly, there is a finite amount of time that cells can survive and cumulative damage incurred from life choices can increase or decrease the speed of cellular damage. (apeironzoh.com)

Exercise can produce Irisin and Semaphorin-3A by stimulating muscle and nerve cell, which have positive effects on other tissues, including the lungs. (jeoct.com)
The light must be absorbed by the endogenous chromophores of cells or tissues for actions. (biomedcentral.com)

Liver kinase B1 inhibits smooth muscle calcification via high mobility group box 1. (omicsdi.org)

CD34 is expressed on early precursor cells of myeloid and B-cell lineages, and CD15 is expressed on immature monocytic lineage cells and highly expressed on granulocytic lineage cells.31 As diseased muscle contains many myeloid cells such as neutrophils, monocytes, and macrophages, a more specific marker for mesenchymal cells with adipogenic potential is AAF-CMK required for detailed characterization of these cells in human diseased muscle. (phytid.org)

Recently, the signaling proteins involved in LPLI-induced proliferation merit special attention, some of which are regulated by mitochondrial signaling. (biomedcentral.com)
LPLI-induced cell cycle progression can be regulated by the activation or elevated expressions of cell cycle-specific proteins. (biomedcentral.com)
Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
This decreases risk for various chronic diseases by eliminating damaging proteins, improving strength, and increasing muscle mass. (apeironzoh.com)

Identifying biomarkers that can differentiate IM from other muscle disorders may elucidate the pathophysiology of IM, guide novel therapies, monitor disease activity/response to treatments and predict prognosis. (biu.ac.il)
Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)

Adult and aged rat myooids had only ~35-60% of the skeletal muscle MHC content of control skeletal muscle from rats, the remaining MHC content consisting of isoforms found in cultured fibroblasts but not in control skeletal muscle. (josam.org)

Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)
To construct a risk map of different human organs, researchers at Shanghai Jiao Tong University analyzed the single-cell RNA sequencing (scRNA-seq) datasets derived from major human physiological systems, including the respiratory, cardiovascular, digestive, and urinary systems. (rna-seqblog.com)

Cardiac muscle lies between the epicardium and the endocardium in the heart. (wikipedia.org)
Cardiac muscle cells generally only contain one nucleus, located in the central region. (wikipedia.org)
Unlike skeletal muscle, cardiac muscle cells are unicellular. (wikipedia.org)
Both smooth muscle cells and cardiac muscle cells have a single nucleus, and skeletal muscle cells have many nuclei. (wikipedia.org)
These contractions in cardiac muscle will pump blood throughout the body. (wikipedia.org)

(C) Scatter plots revealed that the cluster of cells with ACE2 expression also expressed canonical markers of respiratory epithelial cells PIGR and MUC1. (rna-seqblog.com)
Finally, a new class was defined called carcinoma with pleomorphic, sarcomatoid, or sarcomatous elements, which brings together a number of proliferations characterized by a spectrum of epithelial to mesenchymal differentiation. (ersjournals.com)

Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
identification of cells equivalent to these mesenchymal progenitors in humans has considerable clinical implication. (phytid.org)

Thus, these markers in combination with basal lamina staining were useful for the identification of human satellite cells. (phytid.org)
In the oral mucosa, melanocytes are observed in a ratio of about 1 melanocyte to 10 basal cells. (medscape.com)

Cell Metabolism 2013;17(2):210-24. (scielo.br)

Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)

We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. (nature.com)
Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)

These phases include the inflammatory response, the activation, differentiation, and fusion of satellite cells, and the maturation and remodeling of newly formed myofibrils. (wikipedia.org)
But modern cell-type-specific dyes and genetic markers have shown that the dying nuclei other researchers had detected were in fact inflammatory and other cells recruited to atrophic muscle. (robert-gorter.info)
Indeed, several human diseases are characterized by attenuated repair responses and imbalances in the inflammatory response with increased number of infiltrating macrophages [ 3 - 5 ]. (hindawi.com)
They have proinflammatory functions: they produce effector molecules (reactive oxygen and nitrogen intermediates) and inflammatory cytokines (IL-1 β , TNF α , and IL-6) and participate as inducer and effector cells in polarized Th1 responses. (hindawi.com)
Background Inflammatory myopathies (IM) are a heterogeneous group of disorders characterized by autoimmune inflammatory destruction of skeletal muscles. (biu.ac.il)

The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)
Evaluate myogenic ability and functional differences of embryonic, fetal, juvenile and adult MuSCs in fusion, proliferation, engraftment and repopulation assays in mouse models of muscle disease. (ca.gov)
In this project, the group intends to investigate the nature and the role of adenosine and AR signalling pathways on the two isoforms of muscle AChRs: the embryonic (g-AChR) regulating synaptogenesis and muscle development and the adult (e-AChR) with a role in nerve-muscle communication. (units.it)

These constructs (mm 3 -cm 3 scale) consisted of tens to hundreds of long parallel myofiber bundles, containing densely packed, highly viable, and aligned muscle cells. (nature.com)
30-32] The satellite cell was first described by Mauro in 1961 using electron microscopy[33] and later by Bishoff in 1986 utilizing phase-contrast microscopy on solitary myofiber explants. (biotech-angels.com)

45 47 48 It took about fifty years using their initial identification in the first 1960s for genuine preparations of mouse satellite television cells to become isolated and tested for his or her URMC-099 regenerative potential. (biotech-angels.com)

It's been suggested that miRNAs regulate up to 50% of all mRNAs in the human genome. (genengnews.com)
Moreover, miRNAs play a modulatory role in the development and growth of skeletal muscles [ 22 ]. (biomedcentral.com)
Three miRNAs, miRNA-1, −133 and −206, are specifically expressed in muscle and are considered to be myomiRs [ 23 , 24 ]. (biomedcentral.com)
The aims of this study were to establish a mouse BAT-enriched miRNA profile and compare this with miRNAs measured in human BAT. (biomedcentral.com)
We also identified 145 miRNAs expressed in both mouse and human BAT, of which 25 were enriched in mouse BAT. (biomedcentral.com)
For the first time, 69 miRNAs were identified in human BAT but absent in mouse BAT, and 181 miRNAs were expressed in mouse but not in human BAT. (biomedcentral.com)
The present study has identified a small sub-set of miRNAs common to both mouse and human BAT. (biomedcentral.com)
The large number of miRNAs found only in mouse BAT or only in human BAT highlights the differing molecular profile between species that is likely to influence the functional role of BAT across species. (biomedcentral.com)

A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. (nature.com)
Among the many different therapeutic approaches, including exercise, hormone, nutritional therapy, and/or a combination thereof, no one approach has emerged as superior in animal or human studies, and the great variability in the clinical outcomes of patients treated with these approaches has prevented identification of the most effective therapy ( 7 , 8 ). (frontiersin.org)

Skeletal muscle myosin heavy chain (MHC) content was measured and MHC isoforms were separated on SDS-PAGE gels and quantified. (josam.org)
In addition, myooids expressed only developmental isoforms of skeletal muscle MHC, known to generate less specific force than adult isoforms. (josam.org)

This strategy has been applied to develop a three-dimensional (3D) human muscle model with endothelium specificity and endomysium for the study of fibrosis 22 . (nature.com)

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)

While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)

Mice develop tumors resembling those seen in humans, including expression of high levels of miR-21. (genengnews.com)
31] Cells isolated from mature skeletal muscles displayed homogenous expression of Pax7 and contributed to both dietary fiber repair also to the muscle satellite television cell URMC-099 compartment subsequent their transplantation into dystrophic mice. (biotech-angels.com)
At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)
Mice with muscle-speci?c inactive AMPK (AMPK DN) were used to invest. (uncg.edu)

We will identify unique cell surface markers on in vivo derived MuSCs that distinguish PAX7-positive cells from other muscle cells and across cell states. (ca.gov)
30] The additional approach used a transgenic reporter mouse for Pax3 a paralog of Pax7 which allowed for the immediate isolation of Pax3+ (GFP+) muscle satellite television cells by movement cytometry. (biotech-angels.com)
Furthermore these authors demonstrated that Pax7(+)luciferase(+) mononuclear cells could possibly be readily re-isolated offering proof for the self-renewal of the cell human population. (biotech-angels.com)

Clinical studies of androgen supplementation in age-related diseases and muscle wasting are a focus of emerging interest ( 11 ). (frontiersin.org)
16-18] Regardless of the stimulating findings obtained using the dystrophin-deficient mouse super model tiffany livingston clinical studies performed within a cohort of DMD individuals were disheartening because of poor myoblast transfer efficacy and failure to boost strength in treated muscles. (biotech-angels.com)
Because of this alternative use of MSCs, Caplan and Sorell [ 14 ] suggested a renaming of MSCs to medicinal signaling cells to suggest a new era of MSC clinical relevance due to their immunomodulatory properties. (biomedcentral.com)
Use something with zero data on humans and had sh*t results in the one preclinical rodent model published on it, or use something with lots of clinical data that has proven for years to be one of the most well tolerated mass building agents we can deploy? (moreplatesmoredates.com)

Hepatocyte growth factor receptor (c-Met), a member of tyrosine protein kinase receptors (TPKR), is phosphorylated during LPLI-induced proliferation, but tumor necrosis factor alpha (TNF-alpha) receptor has not been affected. (biomedcentral.com)
Cell invasion is a crucial step of tumor metastasis , finding new regulators of which offers potential drug targets for cancer therapy. (sdbonline.org)

These and other data argue against the current interpretation of the myonuclear domain hypothesis and suggest that once a nucleus has been acquired by a muscle fiber it persists. (frontiersin.org)

Macrophages induce phagocytosis of the cell debris. (wikipedia.org)
These macrophages can also facilitate the proliferation and differentiation of satellite cells. (wikipedia.org)
Alternatively activated M2 macrophages comprise cells exposed to low concentrations of M-CSF in the presence of IL-4, IL-13, or IL-10. (hindawi.com)

Develop transcription factor over-expression in combination with improved directed differentiation strategies to generate MuSCs from hPSCs with genetic and functional signatures of the in vivo MuSCs. (ca.gov)

Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)

Cell damage was assessed using MTT assays, and the lactate dehydrogenase (LDH) release rate and flow cytometry were used to detect the accumulation of reactive oxygen species (ROS) and mitochondrial membrane potential respectively. (sdbonline.org)

Immunohistochemistry indicated that bFGF significantly increased the number of satellite cells in the thyroarytenoid muscle up to day 14 and that of neuromuscular junctions on days 28 and 56. (biomedcentral.com)
A single, early local administration of high-dose bFGF prevented atrophic changes in the thyroarytenoid muscles by activating satellite cell proliferation and reforming neuromuscular junctions. (biomedcentral.com)
In this light, they will be useful for identification of new pharmacological tools to control the activity of muscle AChRs and, thus, for finding novel strategies for neuromuscular diseases associated with altered neuromuscular transmission. (units.it)

Such lack of clarity can be attributed to difficulty in isolating the individual events responsible for alterations in skeletal muscle, most of which occur simultaneously, among the multiple age-associated changes and co-morbidities associated with advanced age. (frontiersin.org)

Zou X, Chen K, Zou J, Han P, Hao J, Han Z. (2020) Single-cell RNA-seq data analysis on the receptor ACE2 expression reveals the potential risk of different human organs vulnerable to 2019-nCoV infection . (rna-seqblog.com)

The functional unit of a muscle fiber is called a sarcomere. (wikipedia.org)
We also demonstrated the feasibility of using these 3D bioprinted human skeletal muscle constructs to treat critical-sized muscle defect injuries with structural and functional restoration in a rodent model 2 . (nature.com)
Several types of adenosine receptors have been detected in developing and differentiated skeletal muscle cells, but little is known about the functional outcome of AR modulation on muscle AChRs. (units.it)
Functional three-dimensional skeletal muscle constructs (myooids) were engineered from myogenic cells harvested from the muscles of adult and aged rats. (josam.org)

Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. (sciepub.com)

Many nuclei are present in each muscle cell placed at regular intervals beneath the sarcolemma. (wikipedia.org)
Skeletal muscle is fairly unique in that the mature cells are syncytial and can contain hundreds of nuclei. (frontiersin.org)
But according to a review published in Frontiers in Physiology, modern lab techniques now allow us to see that nuclei gained during training persist even when muscle cells shrink due to disuse or start to break down. (robert-gorter.info)

Based on their contractile and metabolic phenotypes, skeletal muscle can be classified as slow-oxidative (Type I) or fast-oxidative (Type II). (wikipedia.org)
3 metabolic equivalents [METs], which is defined as the ratio of the work metabolic rate to the resting metabolic rate) intensity estimated by accelerometer is positively correlated with lower body muscle size and function in older adults. (researchgate.net)
After 1935 the best method of discovering and measuring the protein-building action of androgenic steroids in humans proved to be metabolic balance studies. (moreplatesmoredates.com)

Recent mechanistic insights into the trophic activities of these cells point to ultimate regulation by nitric oxide, nuclear factor-kB, and indoleamine, among other signaling pathways. (biomedcentral.com)
Yamaguchi DT, Huang JT, Ma D. "Regulation of gap junction intracellular communication by pH in MC3T3-E1 osteoblastic cells. (sciepub.com)

These aged male rats constitute only the surviving 15% of the original cohort of Wistar rats, and for the purposes of aging research are considered equivalent to human beings of ~90 years of age or older (US Dept. of Health and Human Services, Vital Statistics of the United States, 1994). (josam.org)

Anatomy22

Organisms8

Diseases5

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes26

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena2

Information Science4

Health Care1

Regeneration21

Atrophy17

Tissue41

Myogenic differentiation3

Vitro9

Myogenesis4

Myoblasts11

Regulates1

Stem26

Apoptosis8

Porcine6

Loss of skeletal muscle mass4

Endothelial cells2

Inflammation5

Fibers10

Progenitor4

Hypertrophy10

Osteoblast-like cells3

Sarcopenia9

Adipogenic differentiation1

Myotubes3

Plasticity2

Mechanisms9

Cellular7

Tissues2

Inhibits1

Precursor cells1

Proteins6

Differentiate2

Fibroblasts1

Physiological2

Cardiac5

Epithelial2

Progenitors2

Basal2

Metabolism1

Precursors1

Myofibers2

Inflammatory5

Embryonic3

Myofiber2

Regenerative potential1

MiRNAs8

Therapeutic2

Isoforms2

Fibrosis1

Adipose2

Stimulation1

Mice4

Pax73

Clinical4

Tumor2

Hypothesis1

Macrophages3

Vivo1

Molecular2

Assays1

Neuromuscular3

Occur1

20201

Functional4

Characterization1

Nuclei3

Metabolic3

Regulation2

19941