DifferentiationMultinucleated myotubesRegenerationC2C12TissueBiopsyVitroProgenitor cellsCellsDystrophin-deficientMitochondrialKnockoutGenePromotesOxidativeFusionCellBiopsiesWeaknessCytoplasmSarcopeniaAtrophyAppendicular skeletal muscle massMiceRegulatorOnsetPhenotypeExhibit reducedSkeletal muscle mass indexHumanAcquired by aImmunologyCellularProximalProteinsFibersInherited myopathiesDisordersPrimaryDuchenneAcute
Differentiation26
- The microtubule cytoskeleton is critical for muscle cell differentiation and undergoes reorganisation into an array of paraxial microtubules, which serves as template for contractile sarcomere formation. (elifesciences.org)
- We show that oMAP4 is expressed upon muscle cell differentiation and is the only MAP4 isoform essential for normal progression of the myogenic differentiation programme. (elifesciences.org)
- Upon differentiation, mono-nucleated myoblasts withdraw from the cell cycle and fuse to form syncytial myotubes ( Wakelam, 1985 ). (elifesciences.org)
- Human skeletal myoblast cells were cultured and underwent serial passaging into young and senescent phases and were then treated with C. vulgaris, followed by the induction of differentiation. (iium.edu.my)
- The ability of C. vulgaris to promote myoblast differentiation was analysed through cellular morphology, real-time monitoring, cell proliferation, senescence-associated β-galactosidase (SA-β-gal) expression, myogenic differentiation, myogenin expression, and cell cycle profiling. (iium.edu.my)
- The results obtained showed that senescent myoblasts exhibited an enlarged and flattened morphology, with increased SA-β-gal expression, reduced myogenic differentiation, decreased expression of myogenin, and an increased percentage of cells in the G0/G1 phase. (iium.edu.my)
- In conclusion, C. vulgaris improves the regenerative capacity of young and senescent myoblasts and promotes myoblast differentiation, indicating its potential to promote muscle regeneration. (iium.edu.my)
- The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation. (silverchair.com)
- These results suggest that BC3H1 is a skeletal muscle cell line of ectodermal origin that is defective for commitment to terminal differentiation. (silverchair.com)
- Myoblasts cultured using MyoCult™-SF Expansion Medium can be differentiated into multinucleated myotubes using MyoCult™ Differentiation Kit (Human) . (stemcell.com)
- Promotes myoblast differentiation in C2C12 cells. (tocris.com)
- The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. (cellimagelibrary.org)
- Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)
- Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts. (zen-bio.com)
- Myogenin also identified as myogenic factor 4 is a muscle-specific transcription factorassociated with muscle differentiation and cell cycle. (sigmaaldrich.com)
- Prior research has shown that autophagy and mitophagy are intimately linked to skeletal muscle differentiation and myogenesis. (uwaterloo.ca)
- It is activated at the onset of myoblast differentiation. (antikoerper-online.de)
- however, dfdl3 myoblasts did not achieve terminal differentiation. (bham.ac.uk)
- It was established that Pax7 localises in the cytoplasm of dystrophindeficient myoblasts and high expression is retained during differentiation. (bham.ac.uk)
- Muscle satellite cells are myogenic stem cells whose quiescence, activation, self-renewal and differentiation are influenced by microenvironment oxygen levels. (purdue.edu)
- Expression analyses indicate that HIF1α and HIF2α are preferentially expressed in pre- and post-differentiation myoblasts, respectively. (purdue.edu)
- Analysis of satellite cell dynamics on myofibers confirms that HIF1α/2α dKO myoblasts exhibit reduced self-renewal but more pronounced myogenic differentiation under hypoxia conditions. (purdue.edu)
- Furthermore, KLF15 expression was robustly induced during myoblast differentiation. (bvsalud.org)
- Unbiased transcriptome analysis of muscles on day 7 postinjury revealed downregulated genes involved in cell differentiation and metabolic processes in Klf15KO muscles. (bvsalud.org)
- The FK506-binding protein 51 (FKBP5), a positive regulator of myoblast differentiation, was ranked as one of the most strongly downregulated genes in the Klf15KO group. (bvsalud.org)
- Our findings reveal a positive regulatory role of KLF15 in myoblast differentiation and muscle regeneration by activating FKBP5 expression. (bvsalud.org)
Multinucleated myotubes1
- Moreover, although myoblasts from patients affected with FSHD fully differentiated into multinucleated myotubes, they fused to form either thin and branched myotubes with aligned nuclei or large myotubes with random nuclei distribution. (fshfriends.org)
Regeneration17
- Dock3 KO mice appeared to have poor muscle architecture, locomotive activity, and impaired skeletal muscle regeneration. (mdaconference.org)
- In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
- 20% of the original mass invariably results in functional impairment with limited regeneration capacity, which requires reconstructive surgical procedures such as autologous muscle flap transfers 1 . (nature.com)
- This study is aimed at determining the effects of C. vulgaris on promoting muscle regeneration by evaluating myoblast regenerative capacity in vitro. (iium.edu.my)
- Research concludes ginger extract has the potential to reduce muscle loss by triggering changes in some metabolites and their pathways that could promote muscle regeneration in ageing. (nutraingredients.com)
- PLF was located in satellite cells and/ or myoblasts, which increased in number with continued task performance, supporting our hypothesis that PLF plays a role in muscle repair or regeneration. (cdc.gov)
- Regeneration of muscle after injury normally occurs rapidly with satellite stem cells that reside in the space between the basal lamina and the sarcolemma, playing a major role [ 11 ]. (hindawi.com)
- BACKGROUND: Skeletal muscle (SKM) health is impacted by the aging process and results in delayed regeneration and mitochondrial dysfunction. (wku.edu)
- Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
- More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
- Monthly AMS (Amsterdam Movement Sciences) Mechanobiology meetings RaMBaM: Regeneration and Mechanobiology of Bone and Muscle. (vu.nl)
- However, HIF1α/2α dKO in postnatal satellite cells impairs injury-induced muscle repair, accompanied by a reduced number of myoblasts during regeneration. (purdue.edu)
- Successful muscle regeneration following injury is essential for functional homeostasis of skeletal muscles. (bvsalud.org)
- However, little is known regarding its function in muscle regeneration. (bvsalud.org)
- Additionally, we found that Klf15 knockout (Klf15KO) impaired muscle regeneration following injury in mice. (bvsalud.org)
- Local delivery of FKBP5 rescued the impaired muscle regeneration in Klf15KO mice. (bvsalud.org)
- dystrophic changes (eg, muscle fiber necrosis and regeneration) are seen on biopsy specimens. (msdmanuals.com)
C2C125
- cell separations reported here relied on the different cell types (C2C12 myoblasts, myotubes and MRC-5 fibroblasts) exhibiting different the effective permittivity values. (technuc.com)
- On the basis of the effective DEP-based cell separations reported right here, the same conclusion might be reached regarding splendour between stages of C2C12 myoblast cell difference. (technuc.com)
- Cell civilizations (1) C2C12 mouse myoblast cell range: Cells had been plated at a thickness of 3000 cells/cm2 and expanded in high blood sugar Dulbecco's customized Eagle moderate (DMEM) development moderate (General motors) supplemented with 10% fetal bovine serum (FBS) (HyClone), 2?millimeter of 4-HQN IC50 L-glutamine, 100 products/ml of penicillin and 100?beliefs. (technuc.com)
- t) Superimposed forwards spread plots of land … The forwards scatter plots of land attained for C2C12 myoblasts (n? (technuc.com)
- C2C12 cells, primary myoblasts and myotubes. (sun.ac.za)
Tissue18
- Myoblasts are muscle tissue derived mesenchymal control cell progenitors that have got great potential for make use of in regenerative medication, for cardiomyogenesis grafts and intracardiac cell transplantation especially. (technuc.com)
- More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
- These approaches have achieved bioengineered skeletal muscle tissue fabrication with cellular alignment in vitro, and some showed a degree of therapeutic potential in vivo. (nature.com)
- The goal of this study was to use mice expressing human tissue factor pathway inhibitor (TFPI) on α-smooth muscle actin (α-SMA)(+) cells as recipients of allogeneic aortas to gain insights into the cellular mechanisms of intimal hyperplasia (IH). (nih.gov)
- Mesenchymal cells derived from the dermomyotome condense within connective tissue (mesenchymal) scaffolds to form 2 common muscle masses. (medscape.com)
- Anti-myogenin reactivity isseen in the nuclei of myoblasts in developing muscle tissue. (sigmaaldrich.com)
- Skeletal muscle is a unique tissue composed of multinucleated, post-mitotic fibers. (uwaterloo.ca)
- 2) Cultured meat based on tissue engineering has allowed the creation of cultured muscle, adipose, and multi-component meat. (researchgate.net)
- However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
- Skeletal muscle is the largest tissue in the body. (frontiersin.org)
- Now, researchers at the University of Tokyo report innovative biofabrication of bovine muscle tissue in the laboratory that may help meet escalating future demands for dietary meat. (sciencedaily.com)
- We determined the optimal scaffolding and electrical stimulation to promote contractility and anatomical alignment of the muscle tissue to best simulate steak meat. (sciencedaily.com)
- Our morphological, functional and food feature analyses showed that the cultured muscle tissue holds promise as a credible steak substitute. (sciencedaily.com)
- This mostly means being a selective agonist of androgen receptors in bone and muscle tissue only. (thecni.org)
- S-23 and RAD-140 are the main SARMs that are very potent and closer to steroids because they less easily target muscle and bone tissue receptors. (thecni.org)
- This appears to be the driver for an enhanced metabolic rate, with fatty acid transport into skeletal muscle tissue being enhanced. (thecni.org)
- TNC RNA expression is higher in myoblasts and myotubes compared to skeletal muscle tissue. (lu.se)
- With higher expression in undifferentiated myoblast cells than muscle tissue, it is likely that TNC plays a role in muscle tissue remodelling in humans. (lu.se)
Biopsy6
- Muscle biopsy and genetic testing are the most important tools used in the diagnostic evaluation of patients in whom limb-girdle muscular dystrophy (LGMD) is suspected. (medscape.com)
- METHODS: Thirty-nine healthy men performed a Wingate test followed by a muscle biopsy. (lu.se)
- Cardinal morphologic features of myofibrillar myopathies on muscle biopsy are vacuolated muscle fibers and inclusions that were initially given different names in the 1970s. (medscape.com)
- Diagnosis is by DNA analysis and muscle biopsy. (msdmanuals.com)
- Maximal incremental cycling tests, involuntary electrically stimulated isometric quadriceps-muscle contractions, and biopsy of vastus lateralis muscle. (medscape.com)
- Also, muscle biopsy specimens were not obtained in the STOMP trial to investigate whether changes in mitochondrial content and/or function occur during statin treatment andwhether they relate to muscle complaints and exercise performance. (medscape.com)
Vitro2
- Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. (nature.com)
- The purpose of this study is to investigate the molecular effects of an in vitro SKM myoblast aging protocol in combination with a β-2 adrenergic receptor agonist treatment on the expression of genes related to mitochondrial health. (wku.edu)
Progenitor cells3
- Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
- For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)
- Skeletal muscle contains a number of different progenitor cells, including satellite and mesenchymal progenitor cells. (hindawi.com)
Cells24
- Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
- These constructs (mm 3 -cm 3 scale) consisted of tens to hundreds of long parallel myofiber bundles, containing densely packed, highly viable, and aligned muscle cells. (nature.com)
- This was indicated by higher DNA incorporation of thymidine and a higher number of cells expressing PCNA in intact muscle, accompanied by higher Pax7 levels, all reflecting a higher number of myogenic cells, and suggesting that the increased hypertrophy can be attributed to a higher reservoir of myogenic progeny cells produced in response to the TM. (agri.gov.il)
- Most notably, oMAP4 is required for paraxial microtubule organisation in muscle cells and prevents dynein- and kinesin-driven microtubule-microtubule sliding. (elifesciences.org)
- Skeletal muscles-which enable animals to move-are made up of large elongated muscle cells that span the entire length of the muscle. (elifesciences.org)
- Cells called myoblasts elongate and fuse together at their tips to make the muscle cells. (elifesciences.org)
- The filaments act as a template that helps the sarcomeres to align as the muscle cells form. (elifesciences.org)
- The experiments show that the filaments progressively become more ordered as the myoblasts develop into muscle cells. (elifesciences.org)
- The microtubules in cells that make smaller amounts of this protein were more disorganised, and these cells were unable to fuse with each other to form muscle cells. (elifesciences.org)
- This, in turn, allows the muscle cells to become very long. (elifesciences.org)
- Making more oMAP4 alone is not sufficient to form the elongated muscle cells. (elifesciences.org)
- Skeletal muscle fibre formation requires a coordinated programme of morphological and biochemical changes in the differentiating cells. (elifesciences.org)
- These cells, derived from a nitrosourea-induced mouse brain neoplasm, have some of the morphological characteristics of smooth muscle and have been shown to express the vascular smooth muscle isoform of alpha-actin. (silverchair.com)
- However, when BC3H1 cells differentiate in response to incubation in serum-deprived medium or upon approaching confluence, they express TnT as well as sarcomeric muscle isoforms of MHC, MLC 2 and 3, alpha-Tm, and alpha-actin. (silverchair.com)
- We demonstrate that transgenic AAVR overexpression greatly increases the efficiency of transduction of many diverse cell types, including muscle stem cells, which are normally refractory to AAV transduction. (nature.com)
- Activates senescent muscle stem cells (muSCs) and improves regenerative capacity of aged skeletal muscle. (tocris.com)
- Periostin, on the other hand, was not present in satellite cells and/ or myoblasts. (cdc.gov)
- Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
- Skeletal muscles are the largest cells in the body and are one of the few syncytial ones. (frontiersin.org)
- There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)
- Skeletal muscle is fairly unique in that the mature cells are syncytial and can contain hundreds of nuclei. (frontiersin.org)
- Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)
- However, the in vivo roles of HIFs in quiescent satellite cells and activated satellite cells (myoblasts) are poorly understood. (purdue.edu)
- 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
Dystrophin-deficient2
- Dock3 KO mice on the dystrophin-deficient background exacerbated skeletal muscle and cardiac phenotypes. (mdaconference.org)
- Finally, minidystrophintransfection of both types of myoblasts was utilised to examine the effect, especially in dystrophin-deficient myoblasts. (bham.ac.uk)
Mitochondrial6
- FORM appears to stabilize mitochondrial biogenesis-related genes in AGED myoblasts, indicating improved overall mitochondrial health. (wku.edu)
- To determine whether differences exist in exercise performance, muscle function, and muscle mitochondrial oxidative capacity and content between symptomatic and asymptomatic statin users, and control subjects. (medscape.com)
- Statin use attenuated substrate use during maximal exercise performance, induced muscle fatigue during repeated muscle contractions, and decreased muscle mitochondrial oxidative capacity. (medscape.com)
- This suggests disturbances in mitochondrial oxidative capacity occur with statin use even in patients without statin-induced muscle complaints. (medscape.com)
- [ 4-6 ] Although the mechanisms are poorly understood, statins have been shown to reduce muscle mitochondrial oxidative capacity and content in humans [ 7-10 ] and impair exercise-mediated mitochondrial adaptations in skeletal muscle. (medscape.com)
- The aim of the current study was to examine whether differences exist in aerobic exercise performance, muscle contractile function, and muscle mitochondrial oxidative capacity and content between long-term symptomatic and asymptomatic statin users, and control subjects who did not use a statin drug. (medscape.com)
Knockout1
- Interestingly, double knockout of HIF1α and HIF2α (HIF1α/2α dKO) in embryonic myoblasts results in apparently normal muscle development and growth. (purdue.edu)
Gene4
- The gene is highly expressed in brain and in myoblasts. (antikoerper-online.de)
- Also observed was increased gene expression in skeletal muscle. (thecni.org)
- The disruption of the dystrophin-glycoprotein complex (DGC) is caused by a mutation in the dmd gene, which effects muscle integrity, resulting in progressive muscle degeneration and weakness. (bham.ac.uk)
- Here, we examined microarray datasets from the Gene Expression Omnibus database, which indicated downregulated KLF15 in muscles from patients with various muscle diseases. (bvsalud.org)
Promotes1
- Exercise promotes the formation of intracellular junctions in skeletal muscle between stacks of sarcoplasmic reticulum (SR) cisternae and extensions of transverse-tubules (TT) that increase co-localization of proteins required for store-operated Ca 2+ entry (SOCE). (elifesciences.org)
Oxidative1
- In conclusion, this study shows that myoblasts derived from both clinically unaffected and affected muscles of patients with FSHD are more susceptible to oxidative stress than control myoblasts. (fshfriends.org)
Fusion2
- For this, myoblasts drawn from commercial beef were cultured in hydrogel modules that could be stacked allowing fusion into larger chunks. (sciencedaily.com)
- Myoblasts with KLF15 deficiency showed a marked reduction in their fusion capacity. (bvsalud.org)
Cell11
- TMs had immediate (E17) and later (up to 2 wk posthatch) effects in elevating muscle cell proliferation relative to controls. (agri.gov.il)
- Whereas hypertrophy was similar in both TM groups, cell proliferation and Pax7 levels were more robust in the 6-h muscle, mainly posthatch, suggesting a differential effect of various TM periods on cell reservoir vs. hypertrophy and a high sensitivity of myoblasts to relatively small changes in heat duration with respect to these processes, which is manifested in the short and long term. (agri.gov.il)
- Therefore, the next challenge is to understand how other processes-such as the selective stabilisation of some microtubules and the movement of cell materials along the microtubules-cooperate to control muscle fibre formation. (elifesciences.org)
- The BC3H1 cell line has been used widely as a model for studying regulation of muscle-related proteins, such as the acetylcholine receptor, myokinase, creatine kinase, and actin. (silverchair.com)
- Prof. Myburgh is the Leader of both the Muscle Research Group and the NRF RNA for Integrative Skeletal Muscle Research and the academic head of the Central Analytical Facility for Live Cell Imaging. (sun.ac.za)
- human pluripotent stem cell (hPSC)-derived myoblasts may be cultured on cultureware coated with Corning® Matrigel® hESC-Qualified Matrix. (stemcell.com)
- Histopathological changes including excess collagen deposition around myofibers, cell necrosis, inflammatory cell infiltration, and increased cytokine expression result from eccentric exercise, forced lengthening, exertion-induced injury, and repetitive strain-induced injury of muscles. (cdc.gov)
- Cyanobacteria hydrolysate was assumed to be used as the nutrient and energy source for muscle cell growth. (researchgate.net)
- Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
- Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
- Together, HIF1α and HIF2α are dispensable for muscle stem cell function under normoxia, but are required for maintaining satellite cell self-renewal under hypoxic environment. (purdue.edu)
Biopsies2
Weakness6
- Therefore, these abnormalities could be responsible for the muscle weakness observed in patients with FSHD and provide an important marker for FSHD myoblasts. (fshfriends.org)
- Limb-girdle muscular dystrophy refers to a group of genetic disorders that cause progressive weakness and wasting of the skeletal muscles, predominantly around the shoulders and hips. (medscape.com)
- Most patients present with a history of progressive, symmetric, proximal muscle weakness that starts in childhood to young adulthood. (medscape.com)
- Pelvic muscle weakness is most often the first symptom. (medscape.com)
- They are usually adult-onset diseases with slowly progressive weakness involving proximal (and distal) muscles. (medscape.com)
- Patients typically present with slowly progressive, symmetric, proximal muscle weakness with or without facial involvement and diminished or absent tendon reflexes. (msdmanuals.com)
Cytoplasm1
- There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
Sarcopenia10
- Sarcopenia is characterized by the loss of muscle mass, strength, and function with ageing. (iium.edu.my)
- As well, many older individuals suffer from sarcopenia, a protracted muscle wasting disorder that typically begins after the age of 50 and involves a loss of about 1% of muscle mass per year ( Woo, 2017 ). (frontiersin.org)
- These adverse changes in muscle quantity, muscle quality, and the resulting functional deficits are referred to under a common name of "sarcopenia. (hindawi.com)
- 4 ] used the relative skeletal muscle mass index which is calculated by normalizing appendicular skeletal muscle mass, measured by dual-energy X-ray absorptiometry, to height squared in meters to diagnose sarcopenia. (hindawi.com)
- The European Working Group on Sarcopenia in Older People (EWGSOP) recommends diagnosis of sarcopenia based on the presence of low muscle mass and low muscle strength or low physical performance [ 5 ]. (hindawi.com)
- For the purposes of this paper, we define sarcopenia as originally defined by Rosenberg [ 3 ], an age-related loss of muscle, and view the functional aspects of the most recent definitions as consequences of sarcopenia. (hindawi.com)
- Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
- The European Working Group on Sarcopenia in Older People and the Asian Working Group for Sarcopenia recommend SMI, as measured by the bioelectrical impedance analysis (BIA), and CC as methods for screening skeletal muscle mass. (medscape.com)
- Sarcopenia, which is marked by a loss of skeletal muscle mass and strength, is associated with risks of adverse outcomes. (medscape.com)
- Patients with heart failure have a high rate of sarcopenia, but assessing skeletal muscle mass in these patients is difficult because of the fluid retention they often have. (medscape.com)
Atrophy1
- Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
Appendicular skeletal muscle mass1
- SMI was calculated using the BIA by dividing appendicular skeletal muscle mass by height squared. (medscape.com)
Mice2
- The functional benefits of exercise on SOCE, constitutive Ca 2+ entry and muscle force production were lost in mice with muscle-specific loss of Orai1 function. (elifesciences.org)
- In studies, Activation of Rev-Erba in mice increased exercise capacity by increasing mitochondria counts in skeletal muscle. (thecni.org)
Regulator1
- Krüppel-like factor 15 (KLF15) is a metabolic transcriptional regulator in the muscles. (bvsalud.org)
Onset1
- The guideline provides algorithms for diagnosis based on the clinical phenotype, including pattern of muscle involvement, inheritance pattern, age of onset, and associated manifestations (e.g. contractures, cardiomyopathy, respiratory failure). (medscape.com)
Phenotype3
- It is concluded that the data establish the principle that such grafts effect an alteration of the genetic constitution and phenotype of skeletal muscle and, therefore, might be used to alleviate recessively inherited myopathies. (nih.gov)
- Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)
- Patients with LGMD2B can have a variable MRI picture with involvement of gastrocnemius in Miyoshi myopathy and involvement of glutei as well as anterior and posterior thigh muscle in patients with a LGMD phenotype. (medscape.com)
Exhibit reduced1
Skeletal muscle mass index1
- In patients with heart failure , mid-upper arm circumference (MUAC) and arm muscle circumference (AMC) are more significantly associated with prognosis than guideline-recommended skeletal muscle mass index (SMI) or calf circumference (CC) measurements, research suggests. (medscape.com)
Human9
- Isolated from human muscle of the pectoral girdle. (sciencellonline.com)
- We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. (nature.com)
- These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration. (nature.com)
- This strategy has been applied to develop a three-dimensional (3D) human muscle model with endothelium specificity and endomysium for the study of fibrosis 22 . (nature.com)
- We also demonstrated the feasibility of using these 3D bioprinted human skeletal muscle constructs to treat critical-sized muscle defect injuries with structural and functional restoration in a rodent model 2 . (nature.com)
- The supplement is combined with a basal medium to prepare MyoCult™-SF Expansion Medium for the culture of primary human and hPSC-derived myoblasts. (stemcell.com)
- CYTOO has developed a first-in-class myotube model by fully maturing human primary myoblasts. (cytoo.com)
- ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
- METHODS: Human SKM myoblasts (n = 6 per group) were cultured (3 x 10 5 seeding density) and proliferated to 85% confluency upon extraction. (wku.edu)
Acquired by a1
- These and other data argue against the current interpretation of the myonuclear domain hypothesis and suggest that once a nucleus has been acquired by a muscle fiber it persists. (frontiersin.org)
Immunology1
- The IAL aims to synergise the expertise in muscle and neuromuscular diseases of French teams and the expertise in immunology and inflammation of the Brazilian teams . (institut-myologie.org)
Cellular2
- We investigated the cellular and molecular bases for the promotion of muscle development and growth by temperature manipulations (TMs) during late-term chick embryogenesis. (agri.gov.il)
- Limb-girdle muscular dystrophy (LGMD) protein defects occur in several pathways involved in the biologic function of muscle and can be divided into groups based on cellular localization. (medscape.com)
Proximal1
- Symptoms are slowly progressive, impacting the proximal muscles. (msdmanuals.com)
Proteins3
- Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects on myogenic regulatory factors, insulin growth factor-1, and heat-shock proteins. (frontiersin.org)
- However, in the mid 1990s, other proteins were also found to accumulate in the abnormal muscle fibers, and molecular genetic studies revealed several chromosomal loci. (medscape.com)
- These include proteins associated with the sarcolemma (see image below), proteins associated with the contractile apparatus (see image below), and various enzymes involved in muscle function. (medscape.com)
Fibers1
- Further compounding the loss in function with age is preferential loss of cross-sectional area of stronger and faster contracting type II muscle fibers [ 9 ]. (hindawi.com)
Inherited myopathies1
- Invited review: myoblast transfer: a possible therapy for inherited myopathies? (nih.gov)
Disorders2
- We're currently working on a new website to reflect our current focus on muscle disorders! (cytoo.com)
- KLF15 signaling may be a novel therapeutic target for muscle disorders associated with injuries or diseases. (bvsalud.org)
Primary1
- Over several years, evidence has accumulated to suggest that primary diseases of skeletal muscle, such as Duchenne muscular dystrophy, may be susceptible to this approach. (nih.gov)
Duchenne1
- We previously identified DOCK3 as being strongly upregulated in Duchenne muscular dystrophy (DMD), specifically in the skeletal muscles of DMD patient and mouse skeletal muscles. (mdaconference.org)
Acute1
- These results indicate that TT association with SR-stacks enhances Orai1-dependent SOCE to optimize Ca 2+ dynamics and muscle contractile function during acute exercise. (elifesciences.org)