Loading...
  • ABSTRACT
  • THE ANATOMICAL RECORD 218~128-135(1987) Quantitative Ultrastructure of Histochemically Identified Avian Skeletal Muscle Fiber Types ROBERT S . HIKIDA Program i n Anatomy, Neurobiology, and Developmental Biology, Department of Zoological and Biomedical Sciences, Ohio University, Athens, OH 45701 ABSTRACT A cryostat retrieval method and combined adenosine triphosphatase (ATPase) and acetylcholinesterase (AChase) method were used to study the ultrastructure and innervation of histochemically identified skeletal muscle fibers in different pigeon muscles. (docme.ru)
  • soleus
  • We used 2 muscles from rat: soleus (which has a greater proportion of type I fibers, oxidative) and extensor digitorum longus (EDL, which has a greater proportion of type II fibers, glycolytic). (scielo.br)
  • Here, we found that myostatin increased the phosphorylation of Smad3 in soleus and EDL muscles and promoted an increase in proteolysis in soleus and a decrease in protein turnover in EDL and C2C12 cell culture. (scielo.br)
  • These responses were associated with upregulation of Atrogin-1 in both muscles and C2C12 cells, and LC3 gene only in soleus. (scielo.br)
  • We have applied purified recombinant chick neural and muscle agrin to rat soleus muscle in vivo and obtained the following results. (rupress.org)
  • It also elicited an alteration of skeletal muscle phenotype toward the type I fibers in both of the soleus (35.8 % in the control mice vs. 46.7 % in the PHD2-deficient mice, p (sigmaaldrich.com)
  • In addition, calcineurin and nuclear factor of activated T cell (NFATc1) protein levels were increased in both the gastrocnemius and soleus muscles, suggesting that the calcineurin/NFATc1 pathway was responsible for the type I fiber transition regardless of PGC-1α, which responded minimally to PHD2 deficiency. (sigmaaldrich.com)
  • Single muscle fibers from rabbit soleus and adductor magnus and from semitendinosus muscles were peeled to remove the sarcolemma and then stimulated to release Ca2+ by (a) caffeine application or (b) ionic depolarization accomplished via substitution of choline chloride for potassium propionate at constant [K+] X [Cl-] in the bathing solution. (rupress.org)
  • vivo
  • Thus, the present approach provides a novel, simple, and efficient method for studying the effects of agrin on muscle under controlled conditions in vivo. (rupress.org)
  • These channels exhibited increased activation time constant (τm) and reduced conductance, similar to what has been observed in denervated muscles in vivo, where the density of NaV1.5 was increasing progressively after denervation. (deepdyve.com)
  • slow fiber
  • Both fiber types respond to a single stimulus with a n action potential and twitch, but the slow fiber has longer twitch times than the fast fibers. (docme.ru)
  • Indeed, we found that tacrolimus (FK-506), a calcineurin inhibitor, successfully suppressed slow fiber-type formation in PHD2-deficient mice. (sigmaaldrich.com)
  • Taken together, stabilized HIF-1α induced by PHD2 conditional knockout resulted in the transition of muscle fibers toward a slow fiber type via a calcineurin/NFATc1 signaling pathway. (sigmaaldrich.com)
  • Neural
  • Both neural and muscle agrin bind uniformly to the surface of innervated and denervated muscle fibers along their entire length. (rupress.org)
  • Muscle agrin does not cluster AChRs and at 10 times the concentration of neural agrin does not reduce binding or AChR-aggregating activity of neural agrin. (rupress.org)
  • Electrical muscle activity affects the stability of agrin binding and the number, size, and spatial distribution of the neural agrin-induced AChR aggregates. (rupress.org)
  • Expression of neural agrin in transfected cells followed by its release and deposition on neighboring fibers induced multiple AChR aggregates. (rupress.org)
  • occurs
  • Regeneration is a unique adaptation of skeletal muscle that occurs in response to injury. (ovid.com)
  • Signaling between nerve and muscle occurs at neuromuscular junctions (NMJs), which consist of specialized and precisely apposed pre- and postsynaptic structures separated by a synaptic cleft ( Sanes and Lichtman 1999 ). (rupress.org)
  • sodium
  • Our data evidenced an increase in NaV1.5 channels and the involvement of β subunits in the regulation of sodium current and fiber excitability. (deepdyve.com)
  • González, F. 1999-05-15 00:00:00 The role of methionine residues on the fast inactivation of the sodium channel from toad skeletal muscle fibers was studied with the mild oxidant chloramine-T (CT). (deepdyve.com)
  • Metabolism
  • The three main proteolytic processes involved in the control of muscle protein metabolism in mammals are the lysosomal, the Ca 2+ -dependent, and the ubiquitin-proteasome (UPS) systems. (scielo.br)
  • contrast
  • In contrast, slow twitch fibers have a low activity after alkaline preincubation, and high activity after acid preincubation. (docme.ru)
  • In contrast, detubulated fibers had nonlinear capacitances resembling those of q beta charge, and were not affected by adding tetracaine. (rupress.org)
  • experimental
  • The characterization of skeletal muscle fiber types is important for clinical, functional, and developmental studies because there may be fiber type specificity of the muscles' reactions to various experimental, ontogenetic, and pathologic changes. (docme.ru)
  • Our approach allows quantitative kinetic characterization of Foxo1 and Foxo3A nuclear-cytoplasmic movements in living muscle fibers under various experimental conditions. (physiology.org)
  • Single skinned fibers were obtained from rabbit psoas muscles and were then placed in an experimental chamber containing relaxing solution maintained at 15 degrees C. Isometric tension was measured in solutions containing maximally and submaximally activating levels of free Ca2+ (a) in control fiber segments, (b) in the same segments after partial extraction of TnC, and finally (c) after recombination of TnC into the segments. (rupress.org)
  • With approval of the Institutional Animal Care Committee of the University of Heidelberg (Heidelberg, Germany), we chose the well-established model of murine skinned fiber preparations, using an experimental protocol described before. (asahq.org)
  • segments
  • Fiber segments, obtained from vastus lateralis muscle of human subjects by needle biopsy, were mechanically skinned, and their Ca(2+) release and contractile apparatus properties were characterized. (biomedsearch.com)
  • Isolated segments of fibers were voltage clamped in a triple Vaseline® gap chamber. (deepdyve.com)
  • The readdition of TnC to the extracted fiber segments resulted in a recovery of tension to near-control levels and in the return of the tension-pCa relation to its original position. (rupress.org)
  • voltage
  • Short muscle fibers (less than 1.5 mm) from the m. lumbricalis IV digiti of Rana pipiens were voltage-clamped at -100 mV with a two-microelectrode technique, in normal Ringer's solution containing 10(-6) g/ml tetrodotoxin. (rupress.org)
  • types
  • In the present study, we compared protein turnover among different types of muscle fibers in the presence of myostatin. (scielo.br)
  • We aimed to investigate how the size, number and location of LDs are associated with insulin sensitivity and muscle fiber types, and are regulated by aerobic training and treatment with an erythropoiesis-stimulating agent (ESA) in healthy young untrained males. (physiology.org)
  • percentage
  • The volume percentage sarcotubular system of the slow-twitch fibers was intermediate between and significantly different from that of the fasttwitch and slow tonic fibers. (docme.ru)
  • However, the resistance program did not result in greater relative submaximal performance in individuals with a higher percentage of ST fibers. (ovid.com)
  • nerve
  • In the endomysium, individual muscle fibers (cells) are supported by nearby capillaries and nerve cells. (getbodysmart.com)
  • Aggregation of acetylcholine receptors (AChRs) in muscle fibers by nerve-derived agrin plays a key role in the formation of neuromuscular junctions. (rupress.org)
  • dysfunction
  • A common comorbidity of diabetes is skeletal muscle dysfunction, which leads to compromised physical function. (hindawi.com)
  • Yet, little is known about the impact of acute elevated glucose on the temporal properties of AP-induced Ca 2+ transients and ionic underlying mechanisms that lead to muscle dysfunction. (hindawi.com)
  • significantly
  • In previous studies, we showed that bupivacaine as well as ropivacaine destroy skeletal muscle fibers at the injection site, with ropivacaine being characterized by a significantly lower rate of myotoxicity in comparison with bupivacaine. (asahq.org)
  • multiple
  • 400 muscle fibers from one muscle, and horizontal bars denote average of the means ± S.E. p values were determined with a one-way ANOVA and Dunnett's multiple comparison test. (nih.gov)