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  • antigen
  • Using a murine pre-B cell line that is heterozygous for several loci on chromosome 12, including one encoding the cell surface antigen Ly-18, we found that one of the two Ly-18 antigenic forms was lost at a rate of 1.5 x 10(-5) per cell per generation. (asm.org)
  • In the context of the adaptive immune response, an ever-growing number of studies have investigated the experimental conditions (such as skewing cytokines, antigen-presenting cells, or receptor co-stimulation) that favor the differentiation of T cells into a specific effector T helper (Th) type. (frontiersin.org)
  • We have demonstrated that the expression of the relevant antigen is dependent on the replication and budding of a B-tropic ecotropic murine retrovirus. (aacrjournals.org)
  • Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. (currentprotocols.com)
  • 264.7 cells
  • Pre-treatment of Raw 264.7 cells with 4-PBA (an ER stress-inhibitor) reduced the activation of the ER stress indicators, caspase activation and its downstream poly (ADP-ribose) polymerase (PARP) cleavage, phosphorylation of TBK1 and IRF3 and cytoplasmic co-localization of STING and TBK1. (frontiersin.org)
  • adult
  • To assay expression profiles or functions of microglia, we have developed a method to isolate microglial cells and infiltrating leukocytes from adult mouse brain. (mendeley.com)
  • In the present investigation, we cloned and characterized a murine Sp1-related zinc finger transcription factor cDNA, which is expressed in the adult and developing brain. (jneurosci.org)
  • Carcinogen label-retaining cells were compared with slowly cycling [ 3 H]thymidine label-retaining cells and "maturing" basal cells, two distinct proliferative subsets of adult murine epidermis. (aacrjournals.org)
  • Methods: Primarily cultured fetal skin-derived mast cells (FSMCs) isolated from embryos and adult peritoneal MCs were analyzed for storage and release of granule molecules in response to MC activation. (diva-portal.org)
  • Lack of SIRPα signaling in adult SIRPα-mutant mice resulted in a reduced maturation of B cells in the bone marrow, evident by reduced numbers of semi-mature IgD+IgMhi follicular type-II (F-II) and mature IgD+IgMlofollicular type-I (F-I) B cells, as well as reduced blood B cell numbers. (diva-portal.org)
  • RECEPTOR
  • This chapter describes a method by which activating receptor-mediated calcium signaling can be measured in individual murine NK cells using a flow cytometer fitted with a UV laser. (nih.gov)
  • The cell line was created using bone marrow cells from leptin receptor-deficient mice. (springer.com)
  • The cells are MAC-2 and F4/80 positive and have phagocytic activity similar to primary macrophages from wild-type and leptin receptor-deficient mice. (springer.com)
  • We believe that DB-1 cells provide a dependable tool to study the role of leptin or the leptin receptor in obesity-associated inflammation and immune system dysregulation. (springer.com)
  • When the latent infection reactivates in these immunized animals, infected cells would be recognized by these CTLs through T-cell receptor binding to specific intracellularly derived viral peptides bound to cell surface MHC class I proteins. (asm.org)
  • CD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development. (rupress.org)
  • The CD27/CD70 receptor-ligand pair cooperates with the TCR in the regulation of the peripheral T cell response. (rupress.org)
  • Arnold Kristjuhan, The studies on aryl hydrocarbon receptor expression mechanisms in murine granulosa cells (Arüülsüsivesinike retseptori ekspressioonimehhanismide uurimine hiire granuloosarakkudes), Tartu Ülikool. (etis.ee)
  • Activation of the B cells by LPS is not influenced by the presence of E-S 62/PC, suggesting that they may target some aspect of signaling via the Ag receptor. (jimmunol.org)
  • Here we provide evidence that signal regulatory protein α (SIRPα), an Ig-superfamily ITIM-receptor expressed by myeloid but not by lymphoid cells, is involved in regulating B cell maturation. (diva-portal.org)
  • Adding on to our previous findings of a stromal cell defect in SIRPα-mutant mice was the finding that gene expression of receptor activator of nuclear factor-ĸB ligand (RANKL) was significantly lower in cultured bone marrow stromal cells of SIRPα mutant mice. (diva-portal.org)
  • The ubiquitously expressed cell surface glycoprotein CD47 and its receptor signal regulatory protein α (SIRPα) are members of the immunoglobulin superfamily. (diva-portal.org)
  • peritoneal
  • After six sequential interactions, all cell lines were examined for their susceptibility to lysis mediated by murine peritoneal exudate macrophages activated with liposomes containing muramyl tripeptide phosphatidylethanolamine. (aacrjournals.org)
  • Identification of mast cells from splenic and peritoneal populations has also been hindered by poor FcεR1a and c-Kit resolution. (jimmunol.org)
  • progenitors
  • In particular, staining of B cell progenitors in the bone marrow has proven difficult due to poor CD43 and CD24 staining, both of which are critical to separation of early B cell populations. (jimmunol.org)
  • Subpopulation
  • We have previously cloned the cDNA encoding murine CD27, prepared specific mAbs and observed that murine CD27 is expressed on virtually all thymocytes, with the exception of a subpopulation of CD4-8- precursor T cells. (rupress.org)
  • Here, we demonstrate that DAZL, an RNA-binding protein known to play a key role in germ-cell development, marks a subpopulation of ESCs that is actively transitioning toward naïve pluripotency. (sigmaaldrich.com)
  • tumors
  • Neither macrophage infiltration into s.c. tumors nor the experimental or spontaneous metastatic potentials of the parental tumors differed from the lines established by cells surviving macrophage-mediated lysis. (aacrjournals.org)
  • Constitutive Hedgehog (HH) signaling underlies several human tumors, including basal cell carcinoma (BCC). (pubmedcentralcanada.ca)
  • Finally, topical vitamin D3 treatment of existing murine BCC tumors significantly decreases Gli1 and Ki67 staining. (pubmedcentralcanada.ca)
  • protocol
  • Here, we present a protocol to develop and characterize tolerogenic dendritic cells (TolDCs) and evaluate their immunotherapeutic utility. (jove.com)
  • L929
  • After pseudorabies virus (PRV) infection of murine L929 cells, the cell surface expression of major histocompatibility complex (MHC) class I proteins changes such that the total amount of MHC class I molecules remains relatively constant but the levels of the individual alleles D k and K k vary. (asm.org)
  • Berberine at a concentration above 0.1 mg/mL altered the morphology of L929 cells. (hindawi.com)
  • differentiation
  • Differentiation in murine erythroleukemic cells is arrested at the proerythroblast stage. (aacrjournals.org)
  • The results indicate that murine erythroleukemic cells destined to undergo spontaneous differentiation first undergo commitment, an irreversible process characterized by cells becoming ( a ) capable of producing hemoglobin coupled with ( b ) a loss of their ability to undergo more than six subsequent cell divisions. (aacrjournals.org)
  • When logarithmic growth was maintained continuously, the rate of the spontaneous differentiation increased (20- to 100-fold) due to the higher probability of cell commitment. (aacrjournals.org)
  • 2004) "c-Myc Controls the Balance Between Hematopoietic Stem Cell Self-Renewal and Differentiation", Genes a& Development, 25(24): 2747-63. (patentgenius.com)
  • Together, our results provide first evidence for a specific effect of EE on T cell differentiation and its associated changes in gene expression profile. (frontiersin.org)
  • Endoglin is required for myogenic differentiation potential of neural crest stem cells," Developmental Biology , vol. 308, no. 2, pp. 520-533, 2007. (hindawi.com)
  • Origin and differentiation of natural killer cells. (currentprotocols.com)
  • syngeneic
  • Randomly chosen clones of the murine K-1735 melanoma tumor were used by Raz and Geiger to address the question of whether variations in actin organization in these cells may be related to their lung colonization capability in syngeneic hosts (Cancer Res. (biomedsearch.com)
  • Motility
  • The low-metastatic cell variants displayed large focal adhesion plaques, tightly packed actin bundles, elaborate extracellular networks of fibronectin, and restricted motility. (biomedsearch.com)
  • dendritic cells
  • Both individually and upon their interaction, CD47 and SIRPα have been found to play important role in the homeostasis of T lymphocytes or CD8 - conventional dendritic cells (cDCs) in secondary lymphoid organs. (diva-portal.org)
  • Transfection
  • Co-transfection of the Drosophila SL2 cells using mGIF expression plasmid and reporter constructs having Sp1 binding sites indicated that mGIF represses transcription from a TATA-containing as well as from a TATA-less promoter. (jneurosci.org)
  • Line
  • The MSCV System includes the RetroPack PT67 Packaging Cell Line, which produces high-titer virus able to infect a broad range of mammalian host cells. (clontech.com)
  • The LMP-transfected SHG cells showed a significantly higher invasive capacity than the original cell line. (nih.gov)
  • A nonmalignant fibroblastic cell line, 3T3, exhibited little or no capacity to take up exogenous hematin. (aacrjournals.org)
  • regulators
  • B and T lymphoid commitment is better understood than that of NK cells and a set of key genetic regulators of B and T cells fate have been identified and their expression consigns progeny to that lineage ( Warren and Rothenberg, 2003 ). (frontiersin.org)
  • mammalian
  • They can also be used effectively with most mammalian cell lines. (clontech.com)
  • Heterozygous mammalian cell lines normally express both parental alleles at most autosomal loci. (asm.org)
  • homeostasis
  • Cells in homeostasis metabolize glucose mainly through the tricarboxylic acid cycle and oxidative phosphorylation, while activated cells switch their basal metabolism to aerobic glycolysis. (sigmaaldrich.com)
  • tumor cell
  • Direct demonstration that the response was due to tumor cell activation and not that of contaminating normal B lymphocytes was provided by karyotype analysis and by immunoprecipitation, which showed the restriction of light chains on secreted IgM molecules to the λ isotype. (jimmunol.org)
  • melanoma
  • We have further shown that remarkable variations exist in the adhesive properties and locomotor activity of four K-1735 melanoma cell variants that exhibit distinct metastatic properties. (biomedsearch.com)
  • mechanisms
  • The mechanisms by which NK cells recognize infected cells are under study in many laboratories, but one idea is that cells with reduced levels of major histocompatibility complex (MHC) class I proteins on the cell surface are preferentially attacked by NK cells ( 16 ). (asm.org)
  • Together, these observations demonstrate how tumor cells expressing reduced levels of TRPM2 evade neutrophil cytotoxicity in two interrelated mechanisms-downregulation of neutrophil chemoattractants and blocking of the apoptotic Ca 2+ -dependent cascade. (springer.com)
  • advances
  • Despite these advances in our understanding of effector and regulatory functions of NK cells, many key questions surrounding their origin and development remain. (frontiersin.org)
  • Here we discuss the recent advances that have been made in the field of transcriptional regulation of NK cell lineage commitment. (frontiersin.org)
  • transitional cell carc
  • The significant antitumor efficacy of subcutaneous and oral AS warrants further investigation and suggests that AS may provide a new and effective form of therapy for transitional cell carcinoma of the bladder. (chiro.org)
  • freshly
  • One major advantage of this method is that the calcium response of the minority NK cell population and even smaller NK cell subpopulations can be measured simultaneously from a mixture of freshly prepared total splenocytes without resorting to prior cell sorting or expansion in culture. (nih.gov)