Murine megakaryocyte progenitor cells. Medical search. Frequent questions

In summary a number of reports have shown that manifestation of causes improved proliferation and delayed differentiation of murine hematopoietic stem and progenitor cells. (acancerjourney.info)
We consequently set out to study the manifestation and function of in main human being cluster of differentiation 34 positive (CD34+) hematopoietic progenitor cells. (acancerjourney.info)
and 0.5 ng/ml tumor necrosis factor α(TNFα) (all cytokines from Peprotech) for dendritic (Langerhans cell LC) differentiation. (acancerjourney.info)
For megakaryocyte differentiation cells had been extended for 6 times in StemPro-34 mass media (Life Technology) filled with 1 ng/ml IL-3 1 ng/ml SCF and 50 ng/ml TPO (all from Miltenyi Biotec Bergisch Gladbach Germany) and differentiated in mass media supplemented GF 109203X with 50 ng/ml TPO. (acancerjourney.info)
Differentiation was permitted to move forward for 7-13 times based on lineage and was supervised by FACS for lineage-specific cell surface area markers (Compact disc15 for granulocytes Compact disc14 for monocytes. (acancerjourney.info)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
FLT-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. (topsan.org)
Z100225Known to bind to cells that express the tyrosine kinase receptor, FLT3LG promotes long-term expansion and differentiation of human pro-B cells. (topsan.org)
IL-7 is necessary to specify CD8 lineage differentiation during CD4/CD8 cell fate choice in the thymus by inducing expression of the transcription factor Runx3. (biolegend.com)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
A lncRNA identifies Irf8 enhancer element in negative feedback control of dendritic cell differentiation. (costalab.org)
Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
To investigate the proliferation and differentiation of hematopoietic stem and progenitor cells in their physiologic environment, we use state-of-the-art mouse models. (digs-bb.de)
We hypothesize that the continuous but rare differentiation of quiescent HSCs into rapidly dividing progenitors limits mutation accumulation in the hematopoietic system. (digs-bb.de)
These findings suggest that the CSF used in this study accelerated the differentiation of the granulocytic cells and the proliferation of granulocyte colony-forming units in the spleen. (eurekamag.com)

The most abundant and best characterized of these are EVI1 a 1053 amino acid protein with a well-established oncogenic function and myelodysplastic syndrome 1 (MDS1)/EVI1 which in addition to the entire GF 109203X sequence harbours an is mostly expressed in stem and progenitor cells and is necessary because of their maintenance and proliferation (7-10). (acancerjourney.info)

flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes. (topsan.org)

Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
The group are currently focused on the pivotal role that HOXA cluster genes play in the demarcation and possible emergence of haematopoietic stem cells (HSCs) and their role in cancer. (nottingham.ac.uk)
However, eradication of the leukaemia repopulating cell or stem cell whilst retaining normal HSCs remains a challenge with current experimental therapies. (nottingham.ac.uk)
GFP- and by inference Evi1- positive cells were strongly enriched for cells with multilineage and long-term repopulation potential suggesting that was a highly specific and discriminative marker for murine HSCs. (acancerjourney.info)
Our group studies the fundamental biological process contributing to the retention and liberation of haematopoietic stem cells (HSCs) within specific bone marrow (BM) niches and how these HSCs and the BM niche become altered in disease. (edu.au)
During my post-doctoral training I continued to study the effect of cholesterol metabolism on inflammation and made the novel discovery that apolipoprotein E was highly expressed in haematopoietic stem cells (HSCs) and regulated the production of myeloid cells - published in JCI. (edu.au)
Hematopoietic stem cells (HSCs) are at the top of the hematopoietic hierarchy and can differentiate into all types of mature blood cells throughout life. (digs-bb.de)
We recently unraveled an alternative pathway of thrombopoiesis, in which HSCs directly differentiate into megakaryocytes, bypassing the classical multipotent progenitor intermediates (Morcos, Li, Munz et al. (digs-bb.de)
According to this concept, HSCs are activated by an increased demand for mature blood cells in situations such as systemic infection or severe blood loss. (digs-bb.de)

Hematopoietic stem cells can differentiate into one of two progenitor cells: the common lymphoid progenitor and the common myeloid progenitor. (wikipedia.org)
MEPs derive from the common myeloid progenitor lineage. (wikipedia.org)
Committed myeloid progenitor cells and cells with long term reconstituting potential were lost from bone marrow within four days after poly injection. (biomedcentral.com)
Myeloid Progenitor Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
This graph shows the total number of publications written about "Myeloid Progenitor Cells" by people in this website by year, and whether "Myeloid Progenitor Cells" was a major or minor topic of these publications. (uchicago.edu)
Below are the most recent publications written about "Myeloid Progenitor Cells" by people in Profiles. (uchicago.edu)
Open-Label Phase II Prospective, Randomized, Controlled Study of Romyelocel-L Myeloid Progenitor Cells to Reduce Infection During Induction Chemotherapy for Acute Myeloid Leukemia. (uchicago.edu)

We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
Objectives Seeking to elucidate the origin of these vascular and BBB abnormalities, we studied platelets that are known to play a role in maintaining the integrity of the vasculature and thrombotic pathways linked to this, given they surprisingly contain the highest concentration of mHtt of all blood cells. (bmj.com)
We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
We also have a strong interest in the role of plaque macrophages and pathways contributing to foam cell formation. (edu.au)
Investigating pathways contributing to foam cell formation in the atherosclerotic lesion. (edu.au)
Regulation of hematopoietic stem and progenitor cell mobilization by cholesterol efflux pathways. (edu.au)
In addition, IL-7 controls T-cell size and metabolism through the activation of PI3 kinase-dependent pathways and regulation of glucose metabolism. (biolegend.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

Megakaryocyte-erythroid progenitor cells, among other blood cells, are generated as a result of hematopoiesis, which occurs in the bone marrow. (wikipedia.org)
Most of the blood cells produced in the bone marrow during hematopoiesis come from megakaryocyte/erythrocyte progenitor cells. (wikipedia.org)
Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)
Similar to tissue-resident macrophages, mast cells are of dual ontogeny and are generated during both fetal and adult ("definitive") hematopoiesis. (digs-bb.de)

Consistent with the in vitro functions of IL‑11, in vivo administration of rhIL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors. (rndsystems.com)
Intracytoplasmic neutrophils develop membrane contiguity with the demarcation membrane system, thereby transferring membrane to the megakaryocyte and to daughter platelets. (elifesciences.org)
This phenomenon occurs in otherwise unmanipulated murine marrow in vivo, resulting in circulating platelets that bear membrane from non-megakaryocytic hematopoietic donors. (elifesciences.org)
These findings identify emperipolesis as a new cell-in-cell interaction that enables neutrophils and potentially other cells passing through the megakaryocyte cytoplasm to modulate the production and membrane content of platelets. (elifesciences.org)
Megakaryocytes (MKs) are the cellular source of platelets. (elifesciences.org)
We also explore how these diseases influence the function of circulating cells, namely monocytes, neutrophils and platelets. (edu.au)
Platelets are anucleate blood cells, long known to be critically involved in hemostasis and thrombosis. (comprehensivephysiology.com)
However, the notion that platelets represent immune cells is not broadly recognized in the field of Physiology. (comprehensivephysiology.com)
This article reviews the role of platelets in inflammation and immune responses, and highlights their interactions with other immune cells, including examples of major functional consequences of these interactions. (comprehensivephysiology.com)
Platelets contain a number of integrins and cell adhesion molecules on their surface which bind to both leukocytes and endothelial cells (green). (comprehensivephysiology.com)
Platelets also adhere to endothelial cells and help capture flowing leukocytes from the circulation. (comprehensivephysiology.com)

In adult mice, IL-7/IL-7R signaling up regulates expression of early B cell factor (EBF), and EBF in turn regulates expression of B cell-specific genes required for the transition from lymphoid progenitor to pro-B cells. (biolegend.com)

Distinct requirements for optimal growth and In vitro expansion of human CD34(+)CD38(-) bone marrow long-term culture-initiating cells (LTC-IC), extended LTC-IC, and murine in vivo long-term reconstituting stem cells. (ox.ac.uk)
Such extended long-term culture-initiating cells (ELTC-IC) are of the CD34(+)CD38(-) phenotype, are quiescent, and are difficult to recruit into proliferation, implicating ELTC-IC as the most primitive human progenitor cells detectable in vitro. (ox.ac.uk)
Here, CD34(+)CD38(-) BM ELTC-IC (12-week) were efficiently recruited into proliferation and expanded in vitro in response to early acting cytokines, but conditions for expansion of ELTC-IC activity were distinct from those of traditional (5-week) LTC-IC and murine long-term repopulating cells. (ox.ac.uk)
The distinct requirements for expansion of ELTC-IC activity when compared with traditional LTC-IC suggest that the ELTC-IC could prove more reliable as a predictor for true human stem cell activity after in vitro stem cell manipulation. (ox.ac.uk)
Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
In vitro , IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G 0 period of early hematopoietic progenitors. (rndsystems.com)
Emperipolesis is amplified in models of murine inflammation associated with platelet overproduction, contributing to platelet production in vitro and in vivo. (elifesciences.org)
Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. (ixcellsbiotech.com)
Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
In vitro, MPL515/630 but not MPL515/625 or MPL515/625/630 retained the ability to induce TPO-independent proliferation and increase colony-forming unit megakaryocytes (CFU-Mk). (biomedcentral.com)

Clonal progeny of single Sca1(+) SP cells show cardiomyocyte, endothelial and smooth muscle lineage potential after cardiac grafting, augmenting cardiac function although durable engraftment is rare. (ox.ac.uk)
Alone, flt-3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU-GEMM, and the very primitive high proliferative potential colony-forming cells, HPP-CFC. (topsan.org)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
Single-cell analysis of mixed-lineage states leading to a binary cell fate choice. (uchicago.edu)

It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. (ixcellsbiotech.com)
On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effectors functions of granulocytes, monocytes/macrophages and eosinophils. (ixcellsbiotech.com)

Chronic myelogenous leukemia (CML), also known as chronic myeloid leukemia, is a myeloproliferative disorder characterized by increased proliferation of the granulocytic cell line without the loss of their capacity to differentiate. (medscape.com)

Single-cell RNA-seq unmasked the existence of two subsets within the LT-HSC population that was apparent upon stimulation but not steady-state. (biorxiv.org)
While our initial manuscript focused on our unexpected finding that committed progenitor subsets could not be identified in mitotic progenitors, all reviewers requested further analysis of when distinct interneuron subsets first emerge during development, and how heterogeneity in the eminences could be connected to later timepoints. (authorea.com)

Furthermore, increase in mRNA level of VNN3 was confirmed in murine intestinal epithelial samples with aGVHD. (biomedcentral.com)
Furthermore, these 5 mRNA expressions were tested with real-time polymerase chain reaction (Real-Time PCR) in murine intestinal epithelial samples and VNN3 mRNA expression was validated for GI GVHD. (biomedcentral.com)
These data suggest that GT3 has radioprotective function in intestinal epithelial and crypt cells. (tocotrienolresearch.org)
DNMT3A expression is downregulated in intestinal epithelial cells from IBD patients and upon tumor necrosis factor treatment in murine intestinal organoids. (nature.com)
Genetic deletion of Dnmt3a in intestinal epithelial cells ( Dnmt3a ΔIEC ) in mice confirms the phenotype of an altered epithelial ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in the colon in vivo. (nature.com)
Intestinal epithelial cells (IECs) have evolved to build a complex physiological barrier that separates the intestinal lumen from the underlying mucosal tissue. (nature.com)
In children diagnosed with IBD, genome-wide DNA methylation profiling of purified intestinal epithelial cells clearly distinguishes between healthy individuals and the IBD group 8 . (nature.com)

Here we show that retroviral overexpression of Jak2 V617F mutant into wild-type p53 murine bone marrow cells induced polycythemia vera (PV) in the recipient mice, whereas Jak2 V617F-transduced p53-null mice developed lethal leukemia after the preceding PV phase. (elsevierpure.com)
MEP)) and erythroid progenitors (CD71 + ) from Jak2 V617F-transduced p53-null leukemic mice had leukemia-initiating capacity, however, myeloid differentiated populations (Mac-1 + ) could not recapitulate the disease. (elsevierpure.com)
Drayer AL, Boer AK, Los EL, Esselink MT, Vellenga E. Stem cell factor synergistically enhances thrombopoietin-induced STAT5 signaling in megakaryocyte progenitors through JAK2 and Src kinase. (rug.nl)
Here, we established induced pluripotent stem cells (iPSCs) derived from MF patient samples (MF-iPSCs) harboring JAK2 V617F, CALR type 1, or CALR type 2 mutations. (ashpublications.org)
CAMK2 inhibition was effective against JAK2 inhibitor-sensitive and JAK2 inhibitor-resistant cells. (ashpublications.org)
We investigated the molecular mechanisms underlying the therapeutic effect of CAMK2G inhibition and found that CAMK2G is activated by MPL signaling in MF model cells and is an effector in the MPL-JAK2 signaling pathway in these cells. (ashpublications.org)

Expansion of LTC-IC was most optimal after 7 days of culture, whereas optimal expansion of ELTC-IC activity required 12 days, most likely reflecting the delayed recruitment of quiescent CD34(+)CD38(-) progenitors. (ox.ac.uk)
CD34 + cells were isolated from UCB specimens using a high magnetic gradient MiniMACS purification system (Miltenyi, Sunnyvale, CA). (biomedcentral.com)
This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)

Using single-cell qRT-PCR and clonal analyses, here we define four subpopulations of cardiac progenitor/stem cells in adult mouse myocardium all sharing stem cell antigen-1 (Sca1), based on side population (SP) phenotype, PECAM-1 (CD31) and platelet-derived growth factor receptor-α (PDGFRα) expression. (ox.ac.uk)
Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
3 Myelofibrosis (MF) refers to the Philadelphia chromosome ( BCR-ABL1 )-negative myeloproliferative neoplasm (MPN) originating at the level of the multipotent hematopoietic stem cell. (haematologica.org)
Gastrointestinal acute graft-versus-host disease (GI aGVHD) is a lethal complication following allogeneic hematopoietic stem cell transplantation (HSCT). (biomedcentral.com)
Development of pre-clinical models using stem cell technologies. (nottingham.ac.uk)
Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
They were expanded for three times in serum-free X-vivo 15 moderate (Lonza Basel Switzerland) filled with penicillin streptomycin glutamax (Sigma-Aldrich GF 109203X Seelze Germany) and 50 ng/ml of every fms-related tyrosine kinase 3 ligand (FLT3-L) stem cell aspect (SCF) and thrombopoietin (TPO) (Peprotech Hamburg Germany). (acancerjourney.info)
We then linked defects in cholesterol efflux to enhanced HSC mobilization, published in Cell Stem Cell. (edu.au)
ApoE regulates hematopoietic stem cell proliferation, monocytosis, and monocyte accumulation in atherosclerotic lesions in mice. (edu.au)
We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
Long-term hematopoietic stem cell damage in a murine model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)
Several functional components of the epithelium contribute to this unique role, among them the secretion of a mucus layer and life-long regeneration of the different epithelial cell types from a stem cell compartment, allowing for quick repair of barrier defects 2 . (nature.com)

BAHCC1 silencing leads to decreased cell proliferation and delayed DNA repair. (bvsalud.org)

Here we have synthesized and investigated new variants of sp2-IGLs for their ability to suppress the activation of human monocyte-derived dendritic cells (DCs) by lipopolysaccharide (LPS) signaling through Toll-like receptor 4. (cnrs.fr)
Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
Z100127CD40LG protein is expressed on all B lymphocytes during various stages of development, activated T cells and monocytes, follicular dendritic. (topsan.org)
IL-7 also controls T cell-dendritic cell interactions that are essential for both T-cell homeostasis and activation in vivo . (biolegend.com)

For decades, hematopoietic stem and progenitor cells have been studied primarily through such transplantation-based assays. (digs-bb.de)

Thus, PDGFRα demarcates the clonogenic cardiogenic Sca1(+) stem/progenitor cell. (ox.ac.uk)

Whereas c-kit ligand (KL), interleukin-3 (IL-3), and IL-6 promoted proliferation and maintenance or expansion of murine long-term reconstituting activity and human LTC-IC, they dramatically depleted ELTC-IC activity. (ox.ac.uk)
In contrast, KL, flt3 ligand (FL), and megakaryocyte growth and development factor (MGDF) (and KL + FL + IL-3) expanded murine long-term reconstituting activity as well as human LTC-IC and ELTC-IC. (ox.ac.uk)

Relevant information has now been established on solid tumors and their micro-environments, which include immune, endothelial and mesenchymal cells. (fortunejournals.com)

Pathobiology: Cells and Genes;Cellular and Molecular Haematology. (nottingham.ac.uk)
We propose that powerful machine-learning techniques based on 'joint manifold learning', often used in the 'alignment' of massive imaging datasets to recognized shared high-dimensional features, can be used to recognize shared cellular phenotypes across single cell datasets. (authorea.com)
IPA revealed a network of associated proteins involved in cellular movement, immune cell trafficking, and inflammatory response. (tocotrienolresearch.org)
Additional processes in which mitochondria are involved include heat production, storage of calcium ions, calcium signaling, programmed cell death (apoptosis) and cellular proliferation. (justia.com)

Exploring how the lipidomes of immune cells regulates their function. (edu.au)
This domain interacts with adaptor molecules and activates a cascade which regulates the inflammatory status in cells [12]. (fortunejournals.com)
Pyk2 regulates megakaryocyte-induced increases in osteoblast number and bone formation. (iu.edu)

Thus we considered the hematopoietic system and found that seven days after poly injection, the number of blood cells in peripheral blood decreased drastically. (biomedcentral.com)
In addition, this CSF induced faster recoveries of neutrophils in the peripheral blood and progenitor spleen cells of cyclophosphamide (CY)-treated mice. (eurekamag.com)
Consequently, the peripheral blood cell profile shows an increased number of granulocytes and their immature precursors, including occasional blast cells. (medscape.com)

Megakaryocyte/erythrocyte progenitor cells must commit to becoming either platelet-producing megakaryocytes via megakaryopoiesis or erythrocyte-producing erythroblasts via erythropoiesis. (wikipedia.org)

Z500147FLT3 is a receptor tyrosine kinase that has been shown to play a role in proliferation and survival of hematopoietic progenitor cells as well as. (topsan.org)
In this fashion, IL-7 induces cell activation, survival, and proliferation of T lymphocytes. (biolegend.com)
PEGylated G-CSF (BBT-015), GM-CSF (BBT-007), and IL-11 (BBT-059) analogs enhance survival and hematopoietic cell recovery in a mouse model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)

Recombinant Human IL-11 (Catalog # 218-IL ) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). (rndsystems.com)
Lyophilized recombinant murine Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) remains stable up to 6 months at lower than -70°C from date of receipt. (ixcellsbiotech.com)

Investigation of bone marrow cells showed that these became apoptotic within seven days after poly injection. (biomedcentral.com)
Furthermore, infusion of normal bone marrow cells rescued mice from death induced by loss of THOC5/Fms interacting protein. (biomedcentral.com)
We showed that CAMK2G hetero knockout in the primary bone marrow cells expressing MPL W515L decreased colony-forming capacity. (ashpublications.org)
WO 2016/135723 to the present inventors discloses mammalian bone marrow cells enriched with mitochondria for treatment of mitochondrial diseases. (justia.com)

Integrative genomics analyses reveal that BAHCC1 is a transcriptional regulator controlling expression of E2F/KLF-dependent cell-cycle and DNA-repair genes. (bvsalud.org)
One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)

PDGFRα demarcates the cardiogenic clonogenic Sca1+ stem/progenitor cell in adult murine myocardium. (ox.ac.uk)
In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)

Consequently, BAHCC1 deficiency cooperates with PARP inhibition to induce melanoma cell death. (bvsalud.org)
Inhibition of HOX/PBX dimer formation leads to necroptosis in acute myeloid leukemia cells ONCOTARGET. (nottingham.ac.uk)
The inhibition of NAMPT is usually shown to induce cell death and reduce osteoclastogenesis in MM [10,11]. (forgetmenotinitiative.org)
MF model cells and mice induced by MPL W515L, another type of mutation recurrently detected in MF patients, were used to elucidate the therapeutic potential of CAMK2 inhibition. (ashpublications.org)

Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Stromal cell feeder layers (e.g. (stemcell.com)
Interleukin 11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. (rndsystems.com)

IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. (rndsystems.com)

Bone marrow megakaryocytes engulf neutrophils in a phenomenon termed emperipolesis. (elifesciences.org)
Tethered neutrophils enter in membrane-bound vesicles before penetrating into the megakaryocyte cytoplasm. (elifesciences.org)
Transit through megakaryocytes can be completed as rapidly as minutes, after which neutrophils egress intact. (elifesciences.org)
Almost 50 years ago, it was observed that MKs can engulf other hematopoietic cells, most commonly neutrophils ( Larsen, 1970 ). (elifesciences.org)

The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)

The leukemic mice had severe anemia, hepatosplenomegaly, pulmonary hemorrhage and expansion of dysplastic erythroid progenitors. (elsevierpure.com)

CD4 T cell lymphopenia increases the expression of circulating IL-7, and TGFb induces IL-7 downregulation. (biolegend.com)

Besides CHS, mutation in gene is usually one of the important factors that trigger hemophagocytic lymphohistiocytosis, a insufficiency in resistant program function, and life-threatening disease characterized by uncontrolled macrophage and T-cell account activation [14]. (forgetmenotinitiative.org)
In an ongoing project, we are investigating how this mast cell ontogeny modifies the effect of a somatic kit mutation and may explain the heterogeneous phenotype of mastocytosis. (digs-bb.de)

Both new datasets strongly validate our original findings, producing nearly identical developmental trajectories, and exhibiting no evidence of distinct subpopulations of fate-restricted cells in the subventricular zone. (authorea.com)
Cre ;Ai9 line that uniformly marks interneuron progenitors, We therefore collected a set of 15,522 single cells across a developmental timecourse (E13.5, E18, P10), obtaining 3,432 additional P56 cells from a public resource hosted by the Allen Brain Atlas. (authorea.com)

These cells were used for chemical screening, and calcium/calmodulin-dependent protein kinase 2 (CAMK2) was identified as a promising therapeutic target. (ashpublications.org)

We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)

Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
Loss of mature blood cells as well as systemic inflammation does not stimulate additional HSC contribution. (digs-bb.de)

Nuclear transfer efficiency is enhanced by introduction of compatible cytoplasm or mitochondrial DNA (same species or similar to donor cell or nucleus). (justia.com)
WO 2013/002880 describes compositions and methods comprising bio-energetic agents for restoring the quality of aged oocytes, enhancing oogonial stem cells or improving derivatives thereof (e.g., cytoplasm or isolated mitochondria) for use in fertility-enhancing procedures. (justia.com)

Super-enhancers (SEs) are stretches of enhancers ensuring a high level of expression of key genes associated with cell function. (bvsalud.org)
In parallel, we used a version of the SMART-Seq2 protocol to obtain deep sequencing data (4-7,000 genes/cell) from 400 mitotic progenitors. (authorea.com)
Finally, we added data produced using the 10X genomics system for 7,000 postmitotic progenitors with significantly increased sequencing depth (1,500-11,000 UMI/cell, 1,000-4,000 genes/cell), and observed identical branching patterns as in our original Drop-seq dataset. (authorea.com)
Our results offer a even more unique understanding of the jobs of and genes in the molecular pathogenesis of MM. MATERIALS AND METHODS Cell collection The myeloma cell collection RPMI 8226 was purchased from the American Type Culture Collection (ATCC, USA). (forgetmenotinitiative.org)
This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

Our studies have revealed distinct features of tyrosine sites 625 and 630 in mediating MPL W515L-induced megakaryocyte hyperproliferation and MPNs. (biomedcentral.com)

Schepers H, Geugien M, van der Toorn M, Bryantsev AL, Kampinga HH, Eggen BJ, Vellenga E. HSP27 protects AML cells against VP-16-induced apoptosis through modulation of p38 and c-Jun. (rug.nl)
Cell proliferation was decided using MTT assay, while apoptosis was analyzed with circulation cytometry using Annexin V-fluorescein isothiocyanate/propidium iodide assay. (forgetmenotinitiative.org)
or gene silencing significantly inhibited cell proliferation and induced apoptosis in RPMI 8226 cells ( (forgetmenotinitiative.org)

3) We are defining the properties of cancer stem cells that initiate and sustain the unique disease features of acute leukemias through the use of various adoptive animal models. (stanford.edu)

Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
Although these findings have been confirmed using cell lines, there is a growing evidence to suggest that cell lines do not fully recapitulate the biology of human disease. (biomedcentral.com)
CRISPR-Cas9, an RNA-guided genome editing technology, is revolutionizing cell biology due to the ease and efficiency by which it enables genetic manipulation of mammalian cells. (stemcell.com)
The Gerbaulet group studies the biology of hematopoietic stem and progenitor cells in their native environment. (digs-bb.de)
9 Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA. (jci.org)

A) EM image of an inflamed mouse cremaster venule, demonstrating platelet‐neutrophil‐endothelial cell interaction. (comprehensivephysiology.com)

To get a new insight into pluripotency, we employed a plurifaceted experimental design combining expression proteomics with the proteome-wide integral solubility alteration (PISA) assay 7 to compare pluripotent cells with their isogenic progenies and parental cells as well as with allogeneic cells. (nature.com)
The NAMPT protein manifestation in siRNA-treated cells was estimated by enzyme-linked immunosorbent assay. (forgetmenotinitiative.org)

McDonald's group used several proteins to predict severe GI aGVHD and non-relapse mortality, such as T-cell immunoglobulin and mucin-domain containing-3, soluble tumor necrosis factor-alpha, interleukin 1 receptor-like 1 and interleukin −6 [ 8 ]. (biomedcentral.com)

Anatomy10

Organisms1

Diseases1

Chemicals and Drugs10

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes7

Differentiation21

Expressed in stem and progenitor cells1

Erythroid cells1

HSCs12

Myeloid progenitor7

Pathways8

Hematopoiesis7

Platelets11

Lymphoid progenitor1

Vitro12

Lineage6

Macrophages2

Differentiate1

Subsets2

Intestinal epithelial7

JAK26

CD345

Stem cell14

Proliferation and delayed1

Dendritic4

Assays1

Sca11

Ligand2

Mesenchymal Cells1

Cellular4

Regulates3

Peripheral3

Erythropoiesis1

Survival3

Recombinant2

Bone Marro4

Transcriptional2

Clonogenic2

Inhibition4

Stromal cell3

Colony formation1

Neutrophils4

Vivo3

Mice1

Induces1

Mutation2

Developmental2

Kinase1

Pluripotent Stem1

Inflammation2

Cytoplasm2

Genes6

Tyrosine1

Apoptosis3

Sustain1

Biology5

Endothelial1

Assay2

Tumor1