ProliferationDifferentiationErythroleukemiaInsertionalProstate cancerGerm cellsStudying cell populationsTumor cellsRegulationMorphologyVitroGeneGenomeLinesIsolationCellularSynthesisTranscriptionBiologyHumanVarious biologicalBiologicalProteinCultureGrowthMiceAnalysisDirectLevelsMethodsWallRoleStudiesHighProvideShown
Proliferation1
- We have examined the effects of a stromal cell-derived cytokine designated interleukin 11 (IL-11) on the proliferation of murine hemopoietic progenitors in methylcellulose culture. (shengsci.com)
Differentiation3
- Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
- ALA induced erythroid differentiation of human myelogenous leukemia K562 and murine erythroleukemia MEL cells, leads to hemoglobinization and cellular typical maturation. (longdom.org)
- Hemoglobin synthesis and assembly is largely dependent on coordinated gene expression control, it is well established that the histone deacetylase inhibitor butyric acid activates transcription of heme synthesis an globin mRNA expression, leading to changes in cell morphology and induction of erythroid differentiation of erythroleukemic cell lines. (longdom.org)
Erythroleukemia1
- The genotoxic effects of taxol on mouse erythroleukemia cells. (lafayette.edu)
Insertional3
- Fli-1, a known person in the ETS transcription aspect family members, is also the mark of insertional activation by Friend murine leukemia trojan (F-MuLV) and it is preferentially portrayed in vascular endothelial cells and hematopoietic tissue [6]. (columbiagypsy.net)
- Germ-cell deficient (gcd), an insertional mutation manifested as infertility in transgenic mice. (shengsci.com)
- Toward this end, an insertional transgenic mouse mutant has been isolated that results in abnormal germ-cell development. (shengsci.com)
Prostate cancer2
- Itens: Toxicity evaluation of selenium compounds on human prostate cancer and mouse erythroleukemic cells. (lafayette.edu)
- Toxicity evaluation of selenium compounds on human prostate cancer and mouse erythroleukemic cells. (lafayette.edu)
Germ cells2
- Plant germ cells develop in specialized haploid structures, termed gametophytes. (shengsci.com)
- The respective changes include a modified reproductive behavior of one of the female germ cells (central cell) and an extended lifespan of three adjacent accessory cells (antipodals). (shengsci.com)
Studying cell populations1
- However, the vast majority of biological knowledge has emerged as a consequence of studying cell populations and not individual cells. (justia.com)
Tumor cells1
- Many ORs are expressed by tumor cells and tissues, suggesting that they may be associated with cancer progression or may be cancer biomarkers. (bmbreports.org)
Regulation2
- Modulates cyclin B levels and participates in the regulation of cell cycle progression through the G2 phase CCNDBP1 NM_012142 May negatively regulate cell cycle progression CCNG1 NM_004060 May play a role in growth regulation CCNH NM_001239 Involved in cell cycle control and in RNA transcription by RNA polymerase II. (wikipedia.org)
- Our results thus identify an unexpected noncell autonomous role for mitochondria in the regulation of cellular lifespan and provide a basis for the coordinated development of gametic and nongametic cells. (shengsci.com)
Morphology2
- All of them could disrupt the morphology, internal microscopic and cell wall structures of H. pylori thereby acting as an inhibitor. (bvsalud.org)
- The mechanism of action was found to be the disruption of H. pylori morphology, internal microstructure, and cell wall. (bvsalud.org)
Vitro2
- Toxic and antitumorigenic potential of tamoxifen, raloxifene, and the combination on 4t1 mouse breast cancer cells in vitro and in balb/c-j female mice in vivo. (lafayette.edu)
- Their Nrf2 activation activity was evaluated in 293 T cells in vitro. (bvsalud.org)
Gene7
- For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
- Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
- The modification of the three 5' CCGG 3' sequences at nucleotides +24, +6 and -215, relative to the cap site in this promoter, sufficed to silence the gene in transient expression either in Xenopus laevis oocytes or in mammalian cells, and after the fixation of the E2A promoter-chloramphenicol-acetyltransferase (CAT) gene construct in the genome of hamster cells. (uni-bielefeld.de)
- Reactivation of the methylation-inactivated E2A promoter could also be demonstrated in two BHK21 cell lines (mc14 and mc20), which carried the late E2A promoter-CAT gene assembly in an integrated form. (uni-bielefeld.de)
- In these cell lines the late E2A promoter was methylated and the CAT gene was not expressed. (uni-bielefeld.de)
- Heme synthesis enhances globin formation, not only because it is the precursor of Hb but also by modulating the machinery of gene expression and globin synthesis and coordinating erythroid cell maturation. (longdom.org)
- Additional, the mechanism of inhibition involves disrupting the structure of H. pylori cells, altering gene expression, and interfering with bacterial metabolic pathways. (bvsalud.org)
Genome1
- Microfluidic devices employing active valving to position and isolate cells have allowed for the isolation and genome amplification of individual microbial cells (32). (justia.com)
Lines3
- It was demonstrated in transient expression experiments that the 5' CCGG 3'-methylated late E2A promoter was at least partly reactivated in cell lines constitutively expressing the E1 region of Ad2 or of adenovirus type 5 (Ad5) DNA. (uni-bielefeld.de)
- By transfection of cell lines mc14 and mc20, the reactivating functions were shown to reside in the pAd2E1A-13 S cDNA clone of Ad2 DNA. (uni-bielefeld.de)
- Cytotoxic evaluation of Raloxifene, a protein antibreast cancer drug, on human and mouse cancer cell lines. (lafayette.edu)
Isolation1
- Although these systems provide a high-throughput qPCR readout, they do not address the front end sample preparation and require single cell isolation by FACS or micropipette followed by off-chip processing and pre-amplification of starting template prior to analysis. (justia.com)
Cellular1
- In molecular biology, housekeeping genes are typically constitutive genes that are required for the maintenance of basic cellular function, and are expressed in all cells of an organism under normal and patho-physiological conditions. (wikipedia.org)
Synthesis1
- Recently we demonstrated that downregulation or over expression of ALA dehydratase (ALAD) and porphobilinogen deaminase (PBGD), the second and the third enzymes of the pathway, by specific shRNAs caused a marked alteration in PpIX synthesis in K562 erythroleukemic cells. (longdom.org)
Transcription1
- Accordingly, a goal of microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. (justia.com)
Biology1
- Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
Human2
- The aim of this study was to test for the molecular and functional expression of the non-gastric H + /K + ATPase ATP12A/ATP1AL1 in unstimulated and butyrate-stimulated (1 and 10 mM) human myelomonocytic HL-60 cells, to unravel its potential role as putative apoptosis-counteracting ion transporter as well as to test for the effect of the H + /K + ATPase inhibitor SCH28080 in apoptosis. (karger.com)
- Nude mice were subcutaneously inoculated with cells of the IL-6-producing human lung cancer cell line LC-06-JCK and assessed as a model of cancer-related anemia. (biomedcentral.com)
Various biological1
- IL-6 exerts various biological activities on responding cell populations through its binding to transmembrane IL-6 receptor as well as to soluble IL-6 receptor. (biomedcentral.com)
Biological1
- Single cells represent a fundamental biological unit. (justia.com)
Protein1
- SYCO localizes to the mitochondria, and ultrastructural analysis of mutant central cells revealed that the protein is necessary for mitochondrial cristae integrity. (shengsci.com)
Culture1
- Direct and synergistic effects of interleukin 11 on murine hemopoiesis in culture. (shengsci.com)
Growth1
- Earlier KLHL1 antibody studies have shown that knocking-down Fli-1 prospects to designated growth inhibition and death in erythroleukemic cells, indicating a possible use of Fli-1 like a restorative target to induce tumor suppression [18C20]. (columbiagypsy.net)
Mice1
- LC-06-JCK-bearing mice showed high red blood cell counts and erythropoietin levels as compared to NTB mice, whereas MR16-1 treatment did not affect their levels. (biomedcentral.com)
Analysis4
- Moreover, the device does not allow isolated cells to be washed from the supernatant prior to treatment or analysis. (justia.com)
- Microfluidic systems provide numerous advantages for single cell analysis: economies of scale, parallelization and automation, and increased sensitivity and precision that comes from small volume reactions. (justia.com)
- Considerable effort over the last decade has been directed towards developing integrated and scalable single cell genetic analysis on chip (7, 8). (justia.com)
- The critical step of integrating all steps of single cell analysis into a robust system capable of performing measurements on large numbers of cells has yet to be reported. (justia.com)
Direct1
- The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. (justia.com)
Levels1
- Existing methods for measuring transcript levels in single cells include RT-qPCR (1), single molecule counting using digital PCR (2) or hybridization probes (3, 4), and next generation sequencing (5). (justia.com)
Methods2
- Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. (justia.com)
- In particular, the invention relates to microfluidic devices and their uses and methods for assaying cells. (justia.com)
Wall1
- Engineering an artificial cell wall by self-assembly of polyelectrolytes. (lafayette.edu)
Role1
- Dysregulated continuous production of IL-6 by a distinct cell population plays a pathological role in various inflammatory autoimmune diseases. (biomedcentral.com)
Studies1
- In particular, microfluidic qPCR devices (Biomark Dynamic Array, Fluidigm) have recently been applied to single cell studies (15, 16). (justia.com)
High2
- High-throughput single cell measurement technologies are therefore of interest and have broad applications in clinical and research settings. (justia.com)
- Of these, single cell RT-qPCR provides combined advantages of sensitivity, specificity, and dynamic range, but is limited by low throughput, high reagent cost, and difficulties in accurately measuring low abundance transcripts (6). (justia.com)
Provide1
- Iron is strictly conserved, and iron from the Hb of senescent red blood cells is recycled to provide iron for new red blood cells. (biomedcentral.com)
Shown1
- In addition, ORs were recently shown to be expressed in many nonolfactory tissues and cells, indicating that these receptors have physiological and pathophysiological roles beyond olfaction. (bmbreports.org)