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  • viability
  • Not all of the concentrations tested in the study affected cell viability. (mdpi.com)
  • Automated particle counting / characterization is a crucial component of every research stage because it can quickly characterize cells (and assess their viability), as well as particles in virtually all types of liquids, suspensions, emulsions and powders. (beckman.com)
  • We make specific reference to two assisted reproductive technologies, namely mitochondrial supplementation and somatic cell nuclear transfer, and how modulating the mitochondrial content in the oocyte influences embryo viability and the potential to generate enhanced offspring for livestock production purposes. (publish.csiro.au)
  • New microfluidic and bioplotting devices offer increased resolution, little impact on cell viability and have been tested with several bioink materials including fibrin, collagen, hyaluronic acid, poly(caprolactone), and poly(ethylene glycol). (frontiersin.org)
  • Methods
  • Methods and Results- Peripheral blood CD34 + cells were isolated from total mononuclear cells of patients with limb ischemia by apheresis after 5-day administration of granulocyte colony-stimulating factor. (ahajournals.org)
  • cardiac
  • Recently, human ES cell lines have been established that can be used to investigate cardiac development and the function of human heart cells and to determine the basic strategies of regenerative cell therapy. (ahajournals.org)
  • The effects of inoculating different cell numbers, time of EB adherence to gelatin-coated dishes, and rotation speed for optimal EB formation and cardiac differentiation were investigated. (springer.com)
  • Using 3 × 10 5 cells/ml, 10 rpm rotary speed and plating of EBs onto gelatin-coated surfaces three days after culture, were the best parameters for optimal size and EB quality on consequent cardiac differentiation. (springer.com)
  • These optimized parameters enrich cardiac differentiation in ES cells when using the STLV method. (springer.com)
  • Chen M, Lin YQ, Xie SL, Wu HF, Wang JF (2010) Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method. (springer.com)
  • Reverse transcriptase-polymerase chain reaction indicated that human-specific gene expression of cardiomyocyte (brain natriuretic peptide, cardiac troponin-I, myosin heavy chain, and Nkx 2.5), SMC (smooth muscle actin and sm22α), and endothelial cell (CD31 and KDR) markers were dose-dependently augmented in MI tissue. (ahajournals.org)
  • precursor
  • Primary cortical precursor cultures obtained from embryonic day 12 were exposed to 0 nM, 0.25 nM, 0.5 nM, 2.5 nM, and 5 nM MeHg for 48 or 72 h. (mdpi.com)
  • In the case of cartilage tissue formation, the standard cues to produce tissue from differentiated cells (chondrocytes) or adult stem cells did not translate to the embryonic precursor population ( 9 ). (pnas.org)
  • functional
  • Functional assessments with the use of echocardiography and a microtip conductance catheter at day 28 revealed dose-dependent preservation of left ventricular function by CD34 + cell transplantation. (ahajournals.org)
  • Conclusions- Human CD34 + cell transplantation may have significant and dose-dependent potential for vasculogenesis and cardiomyogenesis with functional recovery from MI. (ahajournals.org)
  • Development
  • HNF-3beta is essential for node and notochord formation in mouse development," Cell 78:561-574,1994. (patentgenius.com)
  • Such restriction of GJIC and the segregation of cells into communication compartment domains are almost always associated with embryogenesis and development. (frontiersin.org)
  • During the last 40 years, awareness of the role of ChEs role in regulating non-neuromuscular cell-to-cell interactions has been increasing such as the ones occurring during gamete interaction and embryonic development. (frontiersin.org)
  • Having patient-specific vascular smooth muscle cells (SMCs) available facilitates the study of disease and development of novel therapeutic interventions. (yale.edu)
  • Multilineage
  • We describe an efficient derivation method and characterization of mesenchymal stem cells (MSCs) from hESCs (hESCd-MSCs) that have multilineage differentiation potential and are capable of producing fat, cartilage, and bone in vitro. (pnas.org)
  • In this study, we generated and characterized an embryonic-derived mesenchymal progenitor population without cell sorting, hESCd-MSCs, and demonstrated the capacity for significant cell expansion and multilineage differentiation. (pnas.org)
  • therapies
  • Current treatment options for degenerative diseases focus on physical rehabilitation or drug therapies, which temporarily mask the effects of cell damage, but quickly lose their efficacy. (frontiersin.org)
  • gene expression
  • In this study, we showed that the evaluated compounds affected osteoblast differentiation both at the level of calcium concentrations in the culture as well as on multiple gene expression. (uu.nl)
  • These cells manifest gene expression profiles that are distinct from those of proliferating mESCs. (pubmedcentralcanada.ca)
  • transduction
  • The autocrine and paracrine factors secreted by the differentiated cells may readily interact with stem cells to lead a direct and efficient transduction of molecular signals that may result in expression of tissue-specific markers. (pnas.org)
  • Viral transduction of a doxycycline inducible EGFP-PAX6 expression vector was used to re-introduce (rescue) PAX6 expression in the PAX6 knock out cells. (biomedcentral.com)
  • adult
  • The potential application of these cells has been further enabled by recent advances in creating embryonic-like stem cells from adult skin cells that can easily be harvested from a patient ( 3 , 4 ). (pnas.org)
  • tumor cells
  • Using anti-CS antibodies, normal stroma was always negative whereas tumoral stroma always showed a positive staining, more intense in the highest grade carcinomas, while tumor cells were negative. (frontiersin.org)