AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
Cell DifferentiationCells, CulturedCell LineStem CellsMice, Inbred C57BLGene Expression Regulation, DevelopmentalSignal TransductionTranscription FactorsRNA, MessengerMolecular Sequence DataGene Expression RegulationCell DivisionSex DifferentiationEmbryonic Stem CellsCell LineageCell ProliferationBase SequenceLeukemia Virus, MurineMice, KnockoutTretinoinDNA-Binding ProteinsMice, Inbred BALB CMice, TransgenicReverse Transcriptase Polymerase Chain ReactionGene ExpressionPhenotypeAntigens, DifferentiationImmunohistochemistryHomeodomain ProteinsOsteoblastsOsteogenesisTranscription, GeneticHematopoietic Stem CellsBone Marrow CellsTumor Cells, CulturedTransfectionAmino Acid SequenceMesenchymal Stromal CellsFlow CytometryAdipogenesisPromoter Regions, GeneticEmbryo, MammalianLeukemia, Erythroblastic, AcuteCell Culture TechniquesTime FactorsMoloney murine leukemia virusNeuronsIn Situ HybridizationMyoblastsTrans-ActivatorsBlotting, WesternKeratinocytesDimethyl SulfoxideGene Expression ProfilingAdipocytesApoptosisMutationMuscle DevelopmentDown-RegulationBasic Helix-Loop-Helix Transcription FactorsUp-RegulationEpidermisDNA PrimersMice, Inbred StrainsNuclear ProteinsCell CycleHL-60 CellsCell SurvivalDisease Models, AnimalRepressor ProteinsB-LymphocytesMembrane ProteinsModels, BiologicalBone Morphogenetic ProteinsMesodermEpithelial CellsProto-Oncogene ProteinsRecombinant ProteinsFluorescent Antibody TechniqueMyoD ProteinAlkaline PhosphataseHematopoiesisT-LymphocytesGranulocytesNerve Tissue ProteinsBone Morphogenetic Protein 2Leukemia, ExperimentalSpleenMonocytesTransforming Growth Factor betaMorphogenesisCloning, MolecularReceptors, NotchPolymerase Chain ReactionPhosphorylationCore Binding Factor Alpha 1 SubunitCoculture TechniquesMice, Mutant StrainsBlotting, NorthernProtein Binding
VitroEmbryonic stem cell differentiProliferation and differentiationPluripotent StemMesenchymal StemEpithelialGene expressionDifferentiateStromalMyeloidEmbryonal carcinomaLymphoidLevel of differentiationSelf-renewalPopulationsAntibodyPhenotypesInduceRegulationTh17InhibitionHematopoiesisEndothelial2000Stem and progenitorRegenerative medicineCommitsGerminalEpitheliumOsteogenicMaturationFunctionalBoneLupusIsolationAntigenImmuneMiceHMBADistinctPathwaysTranscription factorLineage cellsESCsClonalScaffoldsTissueHumanInflammatoryInflammationNeural StemPhenotypeCytokinesDendriticIPSCsFACSProgenitor cellModulatesBiologyFetalSubsetsRegeneration
Vitro10
  • In this study, we investigated the modulation of pDC differentiation in Flt-3 ligand (Flt3L)-supplemented BM cultures, using highly purified mesenchymal stem cells (MSCs) that were FACS-isolated from murine BM based on surface marker expression and used after in vitro expansion. (nih.gov)
  • In an in vitro study, CD19+ B cells from Id3-/- mice were more differentiated into plasma cells than those from control mice. (bmj.com)
  • Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
  • At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
  • Thymic atrophy was the result of direct action of THC and not based on maternal factors inasmuch as THC was able to induce T cell apoptosis in vitro in fetal thymic organ cultures. (aspetjournals.org)
  • The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. (lu.se)
  • Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. (bvsalud.org)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
Embryonic stem cell differenti2
Proliferation and differentiation2
Pluripotent Stem13
Mesenchymal Stem3
  • Cell-based therapy with mesenchymal stem cells or renal progenitor cells (RPCs) resident in the kidney has appeared to be an effective strategy for the treatment of AKI. (biomedcentral.com)
  • The aim of this study was to compare the osteogenic potential of mesenchymal stem cells obtained from bone marrow (BM-MSC) with those extracted from adipose tissue (AT-MSC) of adult dogs. (scielo.br)
  • Immortalized murine pulp cells (OD-21)6 are considered as the major action mechanism of those lasers.11 undifferentiated mesenchymal stem cells derived from fetal Although this mechanism has not been fully clarified, the most molar papillae7. (bvsalud.org)
Epithelial10
  • Results In IPF, the bronchiolised and enlarged distal airspaces stained for SOX2 are consistent with epithelial differentiation characteristic of conducting airway epithelium. (bmj.com)
  • NRG1α was detected in serous cells within normal submucosal glands and in epithelial cells lining honeycombing areas in IPF, and was not detected in other patients. (bmj.com)
  • Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)
  • Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
  • In this study, we induced iPSCs differentiation into RPCs using a nephrogenic cocktail of factors combined with the renal epithelial cell growth medium. (biomedcentral.com)
  • We showed that the differentiation efficiency of iPSCs to RPCs increased when cultured with renal epithelial cell growth medium after stimulation with a nephrogenic cocktail of factors. (biomedcentral.com)
  • The process is hypothesized to involve epithelial cell dedifferentiation, interstitial cell transdifferentiation and/or activation of quiescent stem cells. (biomedcentral.com)
  • While much work has focused on the failure of epithelial cell populations as a key component of the aging process, additional studies have shown that aging, as a global phenomenon in the lung, also impacts resident endothelial, mesenchymal, and immune cell populations. (karger.com)
  • He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
  • Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. (lu.se)
Gene expression7
  • In the model, the posttranslational modifications and turnover of p45/NF-E2, as mediated by P-JNK, contribute to the control of its homeostatic concentration and consequently, its regulatory functions in the progression of erythroid differentiation and erythroid gene expression. (tmu.edu.tw)
  • Furthermore, we have analyzed the gene expression paterns of iMG cells in both manic and depressive state of patients with bipolar disorders, and revealed state-dependent microglial experession patterns 16 . (nature.com)
  • 2007 ) Polony analysis of gene expression in ES cells and blastocysts. (neurotree.org)
  • He performed some of the first studies to identify transcription factors that regulate the gene expression and differentiation of eosinophils, which are known to play a major role in allergic and asthmatic responses. (ubc.ca)
  • We further detected the gene expression of proinflammatory cytokines in Mφ and genes related to cell differentiation in MSC during the challenge of cPE.Our results demonstrated that the oxidative phosphorylation of Mφ exposed to cPE was significantly decreased when compared with the control group. (stanford.edu)
  • Furthermore, Mφ and MSCs exposed to cPE showed upregulated gene expression levels of glycolytic regulators and Mφ exposed to cPE expressed higher levels of pro-inflammatory cytokines.This study demonstrated the dysfunctional bioenergetic activity of bone marrow-derived Mφ and MSCs exposed to cPE, which could impair the immunoregulatory properties of cells in the bone niche. (stanford.edu)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
Differentiate7
  • Cells from embryonal carcinoma (EC) lines 6050AJ and PCC4.aza 1R differentiate in response to treatment with sodium butyrate as well as retinoic acid (RA) or hexamethylenebisacetamide (HMBA). (rupress.org)
  • A number of micro-evolutionary processes occur within the GCs, leading to the production of high-affinity B cells which acquire the necessary survival signals from T cells and leave the GCs in order to differentiate into plasma cells and memory B cells. (fu-berlin.de)
  • We further demonstrated that similar to non-lymphoid dendritic cells (DCs), CD103(+) LCs originate from pre-DCs, whereas CD11b(+) LCs differentiate from both pre-DCs and monocytic precursors. (nih.gov)
  • Collectively, in a tissue-dependent manner, murine LCs differentiate from at least three distinct precursors (embryonic, pre-DC, and monocytic) in steady state. (nih.gov)
  • When murine erythroleukemia (MEL) cells, containing the transferred rat c‐myc gene under the control of human metallothionein II gene promoter, are induced to differentiate with dimethyl sulfoxide, the level of differentiation is dependent on the c‐Myc level, which is modulated by the addition of Zn ions. (elsevierpure.com)
  • Germinal centers where B cells proliferate and differentiate are poorly developed in all lymphoid tissue, including the spleen. (medscape.com)
  • During development, oligodendrocyte precursor cells (OPCs) differentiate into oligodendrocytes (OLs) that extend processes to contact multiple axons of CNS neurons. (jneurosci.org)
Stromal7
  • Phenotype switching: plasticity and/or differentiation of stromal cells and their progenitors within the tumour microenvironment regulate tumour fate. (europa.eu)
  • Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. (mdpi.com)
  • Sex cord-stromal tumors are groups of tumors composed of granulosa cells, theca cells, Sertoli cells, Leydig cells, and fibroblasts of stromal origin, singly or in various combinations. (medscape.com)
  • A worldwide database analysis revealed that younger age, smaller tumor size, early stage, and granulosa cell tumor histologic type appear to be independent prognostic factors for improved survival in patients with malignant sex cord-stromal tumors who undergo lymph node dissection. (medscape.com)
  • Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. (scielo.br)
  • Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. (scielo.br)
  • We hypothesized that impaired immunoregulation and bone regeneration from inflammatory states are associated with glycolytic reprogramming and mitochondrial dysfunction in macrophages (Mφ) and mesenchymal stromal cells (MSCs).We used the Seahorse XF96 analyzer and real-time qPCR to study the bioenergetics of Mφ and MSCs exposed to cPE. (stanford.edu)
Myeloid3
  • The absence of significant MSC-mediated inhibitory effects on myeloid DC differentiation was confirmed by additional experiments in GM-CSF/IL-4-supplemented BM cultures. (nih.gov)
  • iGal-8KO mice displayed anincreased development of splenomegaly (evaluated by cellularity and weight) compared to iWT.Spleen cells analysis by Flow-cytometry showed no percentage differences among CD4 Tlymphocytes, myeloid cells and their different subpopulations, between iGal-8KO and iWT. (conicet.gov.ar)
  • 8. Lui O, Mak N, Leung K. Conjugated linoleic acid induces monocytic differentiation of murine myeloid leukemia cells. (southernbiotech.com)
Embryonal carcinoma1
  • Sodium butyrate induces histone hyperacetylation and differentiation of murine embryonal carcinoma cells. (rupress.org)
Lymphoid8
  • GCs are transient structures which arise after challenge with a T cell-dependent antigen within secondary lymphoid organs, such as the spleen and lymph nodes. (fu-berlin.de)
  • During the immune response activated B cells migrate to the T cell zones within the secondary lymphoid organs and acquire help from the residing antigen-specific, activated T cells. (fu-berlin.de)
  • The obtained results showed that the blood derived GC B cells immigrate into secondary lymphoid organs and are recruited to the already existing GC reaction. (fu-berlin.de)
  • 3\. To address the question whether the capability to immigrate into secondary lymphoid organs is a GC B cell specific feature, additional transfer experiments were conducted with blood derived CD38hiIgG1+ and CD38hiIgM+ potential memory B cells, which correspond to later stages of GC B cell ontogeny. (fu-berlin.de)
  • Hayday, A.C. γδ T cells and the lymphoid stress-surveillance response. (nature.com)
  • IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells and called CTLA-8. (biolegend.com)
  • In the absence of mature B cells, patients lack lymphoid tissue and fail to develop plasma cells, the cells that manufacture antibodies. (medscape.com)
  • Nanoceria increased spleen lymphoid white pulp cell density in BALB/c but not C57BL/6 mice. (cdc.gov)
Level of differentiation1
  • Sodium butyrate treatment of these cells results in only a modest level of differentiation. (rupress.org)
Self-renewal4
  • Stem cells, including embryonic stem cells (ESCs) and adult stem cells, have the potential of self-renewal and differentiation into multiple cell types and therefore hold great promise in regenerative medicine [ 11 - 13 ]. (biomedcentral.com)
  • Stem cells are characterized by their ability to undergo self-renewal to maintain stem cell reserves, and, when required, to produce new, terminally differentiated cells. (karger.com)
  • To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
  • A broader interest of our laboratory is to understand the molecular regulation of HSCs both from the perspectives of self-renewal and differentiation, and how these features become altered upon disease-causing or disease-associated perturbations. (lu.se)
Populations8
  • In these mice, we evaluated the presentation of lupus-mimicking phenotypes and changes in immune cell populations. (bmj.com)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • In this review, we examine aging as a process dependent on specific changes in molecular pathways within multiple lung cell populations. (karger.com)
  • Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
Antibody4
  • The production of such "class switched" antibodies is important for optimizing the immune response to particular antigens, since the antibody class defines its effector functions, such as complement activation, opsonization, neutralization of bacterial toxins and mast cell activation. (fu-berlin.de)
  • The principal role of these cells, in a healthy host, is the elimination of antibody bound parasites through the release of cytotoxic granule proteins. (wikipedia.org)
  • Peripheral T cells from such mice showed decreased proliferative response to T cell mitogen as well as both T cell and antibody response to HIV-1 p17/p24/gp120 antigens. (aspetjournals.org)
  • However, murine models, including knockout and transgenic mice, have been useful in understanding the mechanisms of B lymphopoiesis, B-cell differentiation, and antibody formation. (medscape.com)
Phenotypes3
  • Conclusions Genetic suppression of Id3 in murine models exacerbated lupus-like phenotypes with aberrant B cell differentiation. (bmj.com)
  • Macrophage differentiation into functional phenotypes influences the fate of nanomaterials taken up by these immune cells. (cdc.gov)
  • In contrast, both macrophage phenotypes show identical nanoceria biotransformation to cerium phosphate nanoneedles and simultaneous nanoceria with ferritin co-precipitation within the cells. (cdc.gov)
Induce2
  • An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. (scielo.br)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
Regulation6
  • Based on the above data and analysis of the chromatin-binding kinetics of p45/NF-E2 and the erythroid gene repressor Bach1 during the early phase of MEL differentiation, we suggest a model for the regulation of erythroid maturation. (tmu.edu.tw)
  • Homeostatic regulation of intestinal epithelia by intraepithelial γδ T cells. (nature.com)
  • The transplantation of iPSC-derived RPCs decreased the levels of biomarkers indicative of renal injury and attenuated the necrosis and apoptosis of renal tissues, but resulted in the up-regulation of renal tubules formation, cell proliferation, and the expression of pro-renal factors. (biomedcentral.com)
  • Activation of Btk results in a cascade of signaling events resulting in calcium mobilization and fluxes, cytoskeletal rearrangements, and transcriptional regulation involving nuclear factor-kappaB (NF-kappaB) and nuclear factor of activated T cells (NFAT). (medscape.com)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
  • Aberrant regulation of blood cell formation underlies a wide range of hematological disorders, including leukemia. (lu.se)
Th1711
  • Results In Id3-/- mice, proportions of effector T cells such as Th1, Th2, and Th17 cells were elevated whereas those of regulatory T cells were lower than in control mice. (bmj.com)
  • Mucosal Langerhans Cells Promote Differentiation of Th17 Cells in a Murine Model of Periodontitis but Are Not Required for Porphyromonas gingivalis-Driven Alveolar Bone Destruction. (bvsalud.org)
  • We tested the contribution of mucosal Langerhans cells (LCs) to alveolar bone homeostasis in mice following oral colonization with a well-characterized human periodontal pathogen, Porphyromonas gingivalis We found that oral mucosal LCs did not protect from or exacerbate crestal alveolar bone destruction but were responsible for promoting differentiation of Th17 cells specific to P. gingivalis. (bvsalud.org)
  • Furthermore, destruction of crestal alveolar bone induced by P. gingivalis colonization occurred regardless of the presence of mucosal LCs or P. gingivalis-specific Th17 cells . (bvsalud.org)
  • Our data indicate that both LCs and Th17 cells are redundant in contributing to alveolar bone destruction in a murine model of periodontitis . (bvsalud.org)
  • designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4 T cells, Th17 cells. (biolegend.com)
  • IL-23, which also activates Stat3, serves to maintain Th17 cells lineage. (biolegend.com)
  • Here, we present evidence that the calcium/calmodulin-dependent protein kinase IV (CaMK4) is increased and required during Th17 cell differentiation. (jci.org)
  • Isolation of naive T cells from a murine model of lupus revealed increased levels of CaMK4 following stimulation with Th17-inducing cytokines but not following Treg, Th1, or Th2 induction. (jci.org)
  • Inhibition of CaMK4 reduced Il17 transcription through decreased activation of the cAMP response element modulator α (CREM-α) and reduced activation of the AKT/mTOR pathway, which is known to enhance Th17 differentiation. (jci.org)
  • Importantly, silencing CaMK4 in T cells from patients with SLE and healthy individuals inhibited Th17 differentiation through reduction of IL17A and IL17F mRNA. (jci.org)
Inhibition3
  • This inhibition was cell contact-dependent and soluble factor-independent, as indicated by trans-well experiments. (nih.gov)
  • In this work, we examined the point of inhibition of differentiation by elevated levels of c‐Myc in one (clone 38‐2) of the typical transformants. (elsevierpure.com)
  • Genetic or pharmacologic inhibition of CaMK4 decreased the frequency of IL-17-producing T cells and ameliorated EAE and lupus-like disease in murine models. (jci.org)
Hematopoiesis1
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
Endothelial1
20003
  • A type of neuroglia, ependymal cells are essential for CSF production and have a cuboidal, multi-ciliated morphology ( Brody et al , 2000 ). (cdc.gov)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
Stem and progenitor1
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Regenerative medicine3
  • We also investigate mechanisms of glial cell development and biology to elaborate regenerative medicine approaches in multiple sclerosis . (igbmc.fr)
  • Unfortunately, regenerative medicine is not yet feasible outside a laboratory setting as available protocols to generate target cells are complicated and expensive. (shinshu-u.ac.jp)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
Commits2
Germinal1
  • Development of B cell memory and generation of high affinity antibodies are crucially dependent on germinal centers (GC). (fu-berlin.de)
Epithelium5
Osteogenic3
  • The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. (scielo.br)
  • On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)
  • AT-MSC submitted to osteogenic differentiation showed higher MTT conversion in formazan than BM-MSC, under the same conditions on days 7 and 21. (scielo.br)
Maturation5
  • Supposedly, such a scheme could lead to faster affinity maturation and a higher diversity of GC B cells. (fu-berlin.de)
  • This enables protection at sites directly at risk but also enables environmental factors to influence the maturation and function of immune structures and cells. (nature.com)
  • Although defects may occur in many steps in B-cell development and maturation, resulting in agammaglobulinemia, the most common and well-described defect is the impaired maturation of the pro-B cells to pre-B cells. (medscape.com)
  • There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
  • There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
Functional2
  • In multiple organs, including the lungs, age-related tissue and organ dysfunction interferes with tissue regeneration, which requires functional stem cells. (karger.com)
  • In the absence of functional Btk, mature B cells that express surface Ig and the marker CD19 are few to absent. (medscape.com)
Bone6
  • Despite their crucial role as a link between innate and adaptive immunity, little is known about the modulation of pDC differentiation by other bone marrow (BM) cells. (nih.gov)
  • The localization and differentiation status of the donor B cells within spleen, bone marrow and mesenteric lymph nodes of recipients were determined during one week after transfer via flow cytometry and immunofluorescence microscopy. (fu-berlin.de)
  • CD4(+) Th cells activated by a microbial biofilm are thought to contribute to the destruction of alveolar bone surrounding teeth by influencing osteoclastogenesis through IL-17A and receptor activator for NF-κB ligand effects. (bvsalud.org)
  • The relative roles of mucosal Ag presentation cells in directing Th cell immune responses against oral pathogens and their contribution to destruction of alveolar bone remain unknown. (bvsalud.org)
  • My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
  • Bone morphogenetic proteins (BMP), known as active components of odontogenic differentiation and tooth development, have been used as a growth factor in an appropriate medium. (scielo.br)
Lupus4
  • Previous studies have reported that Id3 plays an essential role in the development and function of regulatory T cells in lupus. (bmj.com)
  • Additionally, we assessed the influences of B cell-specific Id3 depletion in lupus-induced mice by R848. (bmj.com)
  • Induction using R848 exacerbated lupus-like manifestations including increased proteinuria and higher serum immunoglobulin levels in B cell-specific Id3-depleted mice than in control mice. (bmj.com)
  • Aberrant high expression of B lymphocyte chemokine (BLC/CXCL13) by C11b + CD11c + dendritic cells in murine lupus and preferential chemotaxis of B1 cells towards BLC. (southernbiotech.com)
Isolation2
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
Antigen2
  • 2020 ) Chimeric antigen receptor T-cell therapy after allogeneic stem cell transplant for relapsed/refractory large B-cell lymphoma. (neurotree.org)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
Immune7
  • The immune system consists of many cell types and is highly integrated within other tissues. (nature.com)
  • Activation of immune cells in the brain called microglia has attracted attention as a potential underlying pathological mechanism in chronic pain. (nature.com)
  • Microglia are immune cells in the CNS, and known to have inflammatory functions via releasing proinflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-1β 6 . (nature.com)
  • Together, our data demonstrate for the first time that perinatal exposure to THC triggers profound T cell dysfunction, thereby suggesting that the offspring of marijuana abusers who have been exposed to THC in utero may be at a higher risk of exhibiting immune dysfunction and contracting infectious diseases including HIV. (aspetjournals.org)
  • Through their beneficial interaction with cell surface proteins, fucoidans exert beneficial effects on cells within the immune system, where glycoproteins serve as vital receptors and detectors of changes in the internal environment. (lifeextension.com)
  • Targeted delivery of tumor necrosis factor-α to tumor vessels induces a therapeutic T cell-mediated immune response that protects the host against syngeneic tumors of different histologic origin. (southernbiotech.com)
  • The production of distinct blood cell types can be selectively adapted to meet an individual's acute needs, such as for instance the rapid and enhanced generation of innate immune cells following infection. (lu.se)
Mice8
  • Methods To investigate the role of Id3 in B cells, we generated C57BL/6 mice with a CD19Cre-mediated B cell-specific depletion of Id3 as well as mice (Id3-/-) with a conventional knockout of Id3. (bmj.com)
  • Furthermore, proportions of plasma cells were significantly elevated in Id3-/- mice. (bmj.com)
  • An elevated proportion of plasma cells was replicated in mice with B cell-specific Id3 depletion. (bmj.com)
  • Expression of cell-cycle regulator CDK2-associating protein 1 (p12CDK2AP1) in transgenic mice induces testicular and ovarian atrophy in vivo. (nih.gov)
  • Murine gene mutations in human counterparts may be associated with a clinical illness different from the illness seen in mice. (medscape.com)
  • Furthermore, naive T cells from mice lacking CaMK4 did not produce IL-17. (jci.org)
  • Here, through the use of conditional knock-out mice, we demonstrate a requirement for ILK in oligodendrocyte differentiation and axonal myelination in vivo . (jneurosci.org)
  • Conclusions: BALB/c mice were more responsive to nanoceria-induced effects, e.g. liver caspase-1 activation, reduced liver vacuolation, and increased spleen cell density. (cdc.gov)
HMBA1
  • These results might indicate that the mechanism by which sodium butyrate promotes differentiation of EC cells is different from the ways in which RA and HMBA act. (rupress.org)
Distinct2
  • ii) To monitor the migratory behavior of GC B cell emigrants, different B cell subsets, corresponding to distinct stages of GC B cell ontogeny were isolated and enriched from blood. (fu-berlin.de)
  • Stem cells of all organs - including the lung, which harbors distinct stem cells for each separate tissue that makes up the lung as a whole - reside in niches described as a microenvironment that supports and maintains the 'stemness' of cells as a critical reservoir for maintaining tissue homeostasis and responding to injury [ 3 ]. (karger.com)
Pathways3
  • Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
  • In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
  • We employ screening and targeted testing (CRISPR and small molecule) to ask which genes and pathways are required in B cells and primary lymphoma cells for resistance to lymphoma drugs. (seattlechildrens.org)
Transcription factor4
  • Singularly, SPDEF, a transcription factor associated with mucus metaplasia, was rarely detected in mucus cells in IPF. (bmj.com)
  • Mucus cells lining abnormal airways from patients with IPF expressed MUC5B and SOX2 but lacked SAM pointed domain containing ETS transcription factor (SPDEF) and forkhead box A3(FOXA3). (bmj.com)
  • In the mouse, the differentiation of this new lineage is initiated by TGFbeta-1 and IL-6 or IL-21, which activates Stat3 and induces the expression of the transcription factor retinoic acid-related orphan receptors (RORγt and RORα). (biolegend.com)
  • His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
Lineage cells1
ESCs2
  • The team, led by Associate Professor Tomohide Takaya from the Department of Agricultural and Life Sciences at Shinshu University, recently discovered that an anti-nucleolin aptamer, myogenetic oligodeoxynucleotide iSN04, induced myocardial differentiation in embryonic stem cells (ESCs). (shinshu-u.ac.jp)
  • Using various assays like RNA sequencing, cell staining and imaging, and molecular interaction and pathway analysis, the researchers investigated iSN04's effect on murine ESCs and iPSCs. (shinshu-u.ac.jp)
Clonal1
  • Interestingly, we find that in vivo clonal expansion of P. gingivalis-specific Th cells and induced regulatory T cells does not depend on mucosal LCs. (bvsalud.org)
Scaffolds2
  • This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
  • Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
Tissue2
  • I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
  • I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
Human13
  • To overcome this limitation, we have recently developed a technique to create human-induced microglia-like (iMG) cells from human peripheral blood monocytes. (nature.com)
  • Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
  • The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. (mdpi.com)
  • Using primary human B cells, we developed a murine model for non-Hodgkin's lymphoma. (seattlechildrens.org)
  • editors Culture of human stem cells. (scielo.br)
  • 2019 ) Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding. (neurotree.org)
  • High-resolution electron microscopy was used to investigate the uptake, distribution, and biotransformation of nanoceria in human and murine M1 and M2 macrophages in unprecedented detail. (cdc.gov)
  • We also found that the same ferritin biomineralization mechanism occurs after the uptake of Ce-ions into polarized macrophages and into unpolarized human monocytes and murine RAW 264.7 cells. (cdc.gov)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
Inflammatory3
  • It was also reported to be involved in pro-inflammatory or anti-inflammatory processes, as well as in regulatory T cells differentiation. (conicet.gov.ar)
  • Here, we investigatedthe role of Gal-8 in the murine inflammatory chronic infection induced by T. cruzi (4 months postinfection). (conicet.gov.ar)
  • Mitochondria are highly dynamic organelles that modulate cell metabolism and orchestrate the inflammatory responses that involve both resident and recruited cells. (stanford.edu)
Inflammation1
  • Memory of Inflammation in Regulatory T cells. (ucsf.edu)
Neural Stem1
Phenotype2
  • The major finding of this work was the detection of B cells bearing a GC phenotype in blood. (fu-berlin.de)
  • The detection of GC B cells within blood led to the postulation of the "recirculation" hypothesis, which states that a fraction of GC B cells exits the GCs and enters the peripheral blood without losing the GC B cell phenotype. (fu-berlin.de)
Cytokines1
Dendritic1
  • Plasmacytoid dendritic cells (pDCs) are rare central regulators of antiviral immunity and unsurpassed producers of interferon-α (IFN-α). (nih.gov)
IPSCs1
  • Guiding iPSCs toward a target cell line has garnered much attention, but the process remains challenging. (shinshu-u.ac.jp)
FACS1
  • We have developed and applied FACS-based high-resolution cell fractionation procedures to map the differentiation processes from HSCs. (lu.se)
Progenitor cell1
  • By studying murine transgenic leukemia models developed in our laboratory, we are currently exploring the relationships between hematopoietic progenitor cell identities and the development and progression of acute leukemia. (lu.se)
Modulates1
Biology3
  • I cover mainly cell biology and physiology. (nottingham.ac.uk)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • Frontiers in cell and developmental biology. (lu.se)
Fetal1
  • We noted that murine fetal thymus expressed high levels of the cannabinoid receptors CB1 and CB2. (aspetjournals.org)
Subsets2
  • In contrast, there were no significant differences in other B cell subsets. (bmj.com)
  • This work delivers a deeper insight into the mentioned topics by performing the following analyses: i) A kinetic of the GC B cell subsets was conducted by means of flow cytometric analyses and immunofluorescence microscopy methods. (fu-berlin.de)
Regeneration3
  • Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation. (scielo.br)
  • Current knowledge about the identity and function of these stem cell compartments has been largely drawn from a variety of transgenic and spontaneously mutated mouse models that are characterized by rapid rates of aging or have been used to examine regeneration from injury in the context of natural or accelerated aging. (karger.com)
  • In this review, we will present current data on lung aging and lung regeneration, with specific attention paid to stem cell-driven regeneration within the context of aging. (karger.com)