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  • tumor
  • The characterization of cancer stem cells (CSCs), the identification of oncogene and tumor suppressor genes for CSCs, and the epigenetic study of CSCs and their microenvironments are important topics. (springer.com)
  • The tumor induction technique is based on the MARCM system 10 and exploits the cooperation between an activated oncogene, Ras V12 and loss of cell polarity genes ( scribbled , discs large and lethal giant larvae ) to generate invasive tumors 9 . (jove.com)
  • Recent reports revealed that melanomas, like the majority of tumors, harbor a small subpopulation of cancer stem cells (CSCs), which seem to exclusively fuel tumor initiation and progression towards the metastatic state. (jove.com)
  • DCs pulsed with ALDH high CSC lysates induced significantly higher protective antitumor immunity than DCs pulsed with the lysates of unsorted whole tumor cell lysates in both models and in a lung metastasis setting and a s.c. tumor growth setting, respectively. (jove.com)
  • In particular, splenocytes isolated from the host subjected to CSC-DC vaccine produced significantly higher amount of IFNγ and GM-CSF than splenocytes isolated from the host subjected to unsorted tumor cell lysate pulsed-DC vaccine. (jove.com)
  • In contrast, sebaceous adenoma appears distinct from sebaceous carcinoma in that it is typically a distinctly circumscribed lobular tumor with mature sebocytes and basaloid germinative cells in the periphery. (clinicalpainadvisor.com)
  • glial cells
  • We are interested in understanding the development and function of oligodendrocytes (OLs), the myelin-forming glial cells of the CNS. (jneurosci.org)
  • iPSCs
  • Most recently, his current research is focused on the derivation and characterization of human iPSCs and iPSC-derived neural stem cells from patients of various neurological disease backgrounds in order to facilitate the development of novel cell based assays. (selectbiosciences.com)
  • All of this research led to Kazutoshi Takahashi and Shinya Yamanaka's breakthrough development of induced pluripotent stem cells (iPSCs) in both mouse and human cells in 2006 and 2007, respectively. (addgene.org)
  • iPSCs, much like their ES cell cousins, have the hallmarks of pluripotent cells--they are capable of differentiating into every other cell type in the body and have the capacity to give rise to an entire organism. (addgene.org)
  • iPSC technology removes the possible ethical concerns related to embryonic stem cell use and has the major advantage that iPSCs can potentially be derived from patient-specific or disease-specific cells. (addgene.org)
  • For this reason, iPSCs have become an excellent unlimited stem cell source for therapeutic use. (addgene.org)
  • The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and wait for cells to de-differentiate. (addgene.org)
  • The tables below will help you find plasmids available from Addgene that can be used to create iPSCs or another cell type. (addgene.org)
  • Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). (addgene.org)
  • gene
  • QIAGEN provides a broad range of assay technologies for cell lineage research that enable analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation. (qiagen.com)
  • All organisms exposed to sunlight possess a diurnal biological clock that controls a circadian rhythm of gene expression changes in most cell types. (qiagen.com)
  • Furthermore, we showed that zinc promoted neurite outgrowth via the inactivation of RhoA and led to the induction of neuronal gene expression (MAP2 and nestin) in differentiated stem cells. (hindawi.com)
  • The Desired gene construct is injected in the pronucleus of a reproductive cell using a glass needle around 0.5 to 5 micrometers in diameter. (wikipedia.org)
  • Regenerative
  • The ability to control the transition from an undifferentiated stem cell to a specific cell fate is one of the key techniques that are required for the application of interventional technologies to regenerative medicine and the treatment of tumors and metastases and of neurodegenerative diseases. (springer.com)
  • The use of human embryonic stem cells (ESCs) is a promising approach in the clinical applications of regenerative medicine and cancer research. (springer.com)
  • Types of stem cells used for cardiac regenerative therapy. (hindawi.com)
  • Seed cells are the key components of regenerative medicine, which leads to healing. (hindawi.com)
  • Autologous cartilage is the gold standard for cartilage seed cells in regenerative medicine [ 4 ]. (hindawi.com)
  • 40 . An implant according to claim 22 which is supplemented with a cell type having resorptive and/or regenerative capabilities. (google.com)
  • This "reprogramming" of the cell to an embryonic state holds immense potential for drug development, disease modeling, and regenerative medicine. (addgene.org)
  • dendritic cells
  • We identified cancer stem cell (CSC)-enriched populations from murine melanoma D5 syngeneic to C57BL/6 mice and the squamous cancer SCC7 syngeneic to C3H mice using ALDEFLUOR/ALDH as a marker, and tested their immunogenicity using the cell lysate as a source of antigens to pulse dendritic cells (DCs). (jove.com)
  • Mesenchymal stem cells inhibit generation and function of both CD34 + -derived and monocyte-derived dendritic cells. (springer.com)
  • regeneration
  • Increasing studies have indicated that stem-cell-based cardiovascular regeneration has the potential to revolutionize current treatments for patients with ischemic heart disease [ 4 , 5 ]. (hindawi.com)
  • Much research has examined cartilage regeneration utilizing stem cells. (hindawi.com)
  • clinical
  • Therefore, more pragmatic alternatives, including reprogramming, are required to pave the path for the clinical application of pluripotent stem cells (PSCs) in humans. (springer.com)
  • FEK was diagnosed by clinical appearance and evidence of eosinophil and/or mast cells in corneal cytology. (springer.com)
  • Implanted cells were well-tolerated and effective reducing clinical signs of FEK with a sustained effect during the study period. (springer.com)
  • Together with high quality clinical data, especially on patient follow-up, these cells will be instrumental to better understand the molecular mechanisms behind melanoma progression. (jove.com)
  • In this paper, we assess the current state of stem cell therapy in clinical application, explore the main strategies of cell delivery, and discuss the future direction of the field. (hindawi.com)
  • Until that happens, induced pluripotent cells cannot substitute for adult or embryonic stem cells in clinical medicine or research. (conservapedia.com)
  • immune
  • Cytokines, small signaling proteins secreted primarily by immune cells, activate inter- and intracellular signaling during immune responses. (qiagen.com)
  • The success of this requires efficient drainage of vaccine to lymph nodes where antigen presenting cells can interact with lymphocytes for generation of the wanted immune responses. (jove.com)
  • In this case, a stem cell must be able to produce new blood cells and immune cells over a long term, demonstrating potency. (thefullwiki.org)
  • Cancer
  • This review summarizes the application of cell reprogramming technologies to cancer modeling and treatment and discusses possible obstacles, such as genetic and epigenetic alterations in cancer cells, as well as the strategies that can be used to overcome these obstacles to cancer research. (springer.com)
  • BM-MSC may also contribute to tumour growth by differentiating into cancer associated fibroblasts (CAFs), which form part of the tumour microenvironment. (nuigalway.ie)
  • While genetic changes, or mutations, may arise in any type of cells, probably only those mutations in stem cells can initiate and drive cancer development. (umdnj.edu)
  • Leukaemia - cancer of blood or bone marrow - is caused by mutations that allow defective blood cells to accumulate and displace healthy blood. (innovations-report.com)
  • To develop drugs with a more general efficacy it may therefore be more efficient to target the molecules and pathways shared between different cancer stem cells. (innovations-report.com)
  • Published in Cancer Cell on 8 April 2008. (innovations-report.com)
  • Gupta PB, Chaffer CL, Weinberg RA (2009) Cancer stem cells: mirage or reality? (springer.com)
  • Understanding the mechanisms underlying the regulation of these stem cells has led to many recent breakthroughs in the field of cancer biology. (conservapedia.com)
  • A recent paradigm-shift in cancer biology was the discovery that cancer is a disease resulting from dysregulation of adult stem cells. (conservapedia.com)