• We describe the development of a multiplex PCR that detects four categories of diarrheagenic E. coli and the application of the assay to human diarrheal stools and food in Mexico City. (cdc.gov)
  • We compared light microscopy examination and a semi-nested multiplex PCR [‎SnM-PCR]‎ assay in endemic areas of the Islamic Republic of Iran. (who.int)
  • The current study aimed to evaluate the diagnostic performances of K-ras testing using droplet digital polymerase chain reaction (ddPCR) and a novel single-nucleotide variant (SNV) assay performed on pancreatic EUS-FNA samples. (nih.gov)
  • We have developed a multiplexed, real-time polymerase chain reaction assay targeting mitochondrial genes that is capable of detecting blood meal sources in fleas collected off-host in East Africa. (uncst.go.ug)
  • We evaluated a new multiplex polymerase chain reaction (mPCR), "STDFinder assay", a novel multiplex ligation-dependent probe amplification (MLPA) assay for the simultaneous detection of 7 clinically relevant pathogens of STDs, i.e. (aku.edu)
  • In conclusion, the STDFinder assays have comparable clinical sensitivity to the conventional mono and duplex real-time PCR assay and are suitable for the routine detection of a broad spectrum of these STDs at relatively low cost due to multiplexing. (aku.edu)
  • An investigation of genital ulcers in Jackson, Mississippi, with use of a multiplex polymerase chain reaction assay: high prevalence of chancroid and human immunodeficiency virus infection. (qxmd.com)
  • A multiplex real-time PCR assay was developed to simultaneously genotype plants for the I and bc-12 alleles, which condition resistance in beans to Bean common mosaic virus and Bean common mosaic necrosis virus. (usda.gov)
  • A multiplexed allele-specific polymerase chain reaction assay for the detection of common thiopurine S-methyltransferase (TPMT) mutations. (otago.ac.nz)
  • Purified DNA were analyzed for 37 types of HPV by means of a multiplex polymerase-chain-reaction (PCR) assay targeted to the conserved L1 region of the viral genome, using PGYM09/11 primer pools and primers for ß-globin (33). (cdc.gov)
  • A polymerase chain reaction (PCR) multiplex assay capable of amplifying 13 rapidly mutating Y chromosome short tandem repeats (RM Y-STRs) simultaneously was developed and optimised. (bl.uk)
  • The value of RM-Yplex was demonstrated for differentiating close male relatives in a case where a previously used Y-STR multiplex assay had shown identical haplotypes for those individuals. (bl.uk)
  • A highly sensitive and reliable RM-Yplex multiplex assay has been thus developed, which is expected to help genetic populations studies and forensic casework. (bl.uk)
  • We uncovered two new ITS2 lineages that require further study before resolving their true taxonomic status, and designed a diagnostic polymerase chain reaction (PCR) assay to distinguish five morphologically similar species. (biomedcentral.com)
  • Based on species-specific differences in ITS2 sequences, the multiplex PCR assay developed here can be used to improve the efficiency of vector identification. (biomedcentral.com)
  • Depicted in this 2007 photograph, was Centers for Disease Control and Prevention (CDC) Biologist, Damien Danavall, as he was performing a multiplex real-time PCR (polymerase chain reaction) assay, able to differentiate between four sexually transmitted organisms, responsible for causing genital ulcerations. (cdc.gov)
  • Finally, the developed TaqMan probe multiplex real-time PCR (mqPCR) assay successfully detected 0.003 ng DNA in a pure state and 0.1% target meat in mixed and commercial matrices. (um.edu.my)
  • The ClonoSEQ assay is an in vitro diagnostic that uses multiplex PCR and NGS to identify and quantify certain gene sequences in DNA extracted from bone marrow from patients with ALL or multiple myeloma. (fda.gov)
  • In 2020, RT-PCR multiplex assays were designed that combined multiple gene targets from the Center for Diseases and Control in a single reaction to increase molecular testing accessibility and throughput for SARS-CoV-2 diagnostics. (wikipedia.org)
  • Real-time PCR assays were developed that were specific for each allele, and a multiplex PCR reaction could unambiguously assign F2 plants to one of nine genotypes. (usda.gov)
  • Genotyping was performed using multiplex polymerase chain reaction (PCR) and 5' nucleasereal-time PCR assays. (cdc.gov)
  • Valve multiplexing has many biological applications such as biomolecular separations, enzymatic assays, polymerase chain reactions. (openwetware.org)
  • Several new commercial multiplex polymerase chain reaction (PCR) assays capable of simultaneously testing a single specimen for an array of pathogens that cause blood infections, meningitis, or encephalitis are available. (cdc.gov)
  • For these cases, multiplex PCR assays capable of simultaneously testing a single specimen for an array of pathogens that cause blood infections, meningitis, or encephalitis were used. (cdc.gov)
  • Beacon Designer software is used to design various Polymerase Chain Reaction (PCR) assays, probes, and primers. (va.gov)
  • The technology can also design TaqMan Probes, High Resolution Melting Analysis (HRMA) probes, Molecular Beacons, and Multiplex Real Time PCR Assays. (va.gov)
  • For the first time, here I targeted double gene sites in short-amplicon length multiplex polymerase chain reaction (mPCR) assays for the detection and differentiation of bovine, buffalo and porcine materials in food chain. (um.edu.my)
  • Currently, providers test for MRD using diagnostics called flow cytometry assays or polymerase chain reaction (PCR)-based assays. (fda.gov)
  • We performed PMS2 mutation analysis using long-range polymerase chain reaction and multiplex ligation-dependent probe amplification for 99 probands diagnosed with Lynch syndrome-associated tumors showing isolated loss of PMS2 by immunohistochemistry. (nih.gov)
  • The reaction was been applied to human herpesviruses by nested amplification of fragments of the DNA polymerase genes. (nih.gov)
  • For cholera surveillance of aquatic environments and ballast water, total DNA extraction, followed by V. cholerae PCR, and O1/O139 serogroup and tcpA/ctxA genes by multiplex PCR offers an efficient system, permitting risk analysis for cholera in coastal areas. (umd.edu)
  • Design and optimization of four multiplex groups targeting 14 genes and their alleles on the KIR locus has been completed. (elsevierpure.com)
  • To rapid ly and effective ly detect transgenic components of soybean oil, in the present study, we isolated DNA from transgenic soybean oil by modified method, and employed the multiplex PCR method to identify targeted genes, including CaMV35S promoter, Nos terminator, NPT II , CP4-EPSPS and endogenous gene Lectin. (scirp.org)
  • Multiplex PCR is successfully established by targeted genes which include CaMV35S promoter, Nos terminator, NPTII, CP4-EPSPS and endogenous gene Lectin. (scirp.org)
  • 17. The prevalence of cagA and cagE genes in Helicobacter pylori strains isolated from different patient groups by polymerase chain reaction. (nih.gov)
  • Identification of phylogroup and genes that encodes for virulence factors was done using multiplex polymerase chain reaction (PCR). (biomedcentral.com)
  • The use of molecular methods, especially real-time multiplex polymerase chain reaction (mPCR), has considerably improved the detection of respiratory pathogens [ 12 ]. (hindawi.com)
  • An internal amplification control was included in the mPCR reaction. (aku.edu)
  • Multiplex PCR (mPCR) adalah salah satu aplikasi teknologi PCR yang memungkinkan digunakan untuk deteksi Salmonella sp, Vibrio cholerae, dan Pseudomonas aeruginosa sekaligus dalam sekali reaksi. (ubaya.ac.id)
  • Some of the applications of multiplex PCR include: Pathogen Identification High Throughput SNP Genotyping Mutation Analysis Gene Deletion Analysis Template Quantitation Linkage Analysis RNA Detection Forensic Studies Diet Analysis Chamberlain JS, Gibbs RA, Ranier JE, Nguyen PN, Caskey CT (1988). (wikipedia.org)
  • Nucleic acid hybridization techniques, exploited by colony hybridizations or polymerase chain reaction (PCR), apply a single detection method to a diversity of organisms. (cdc.gov)
  • Such multiplex detection is an appropriate solution to the challenge of finding diarrheagenic E. coli in stools and in food. (cdc.gov)
  • A new approach to simultaneous detection and typing of related agents by the multiplex polymerase chain reaction (PCR) is described. (nih.gov)
  • The results showed high specificity for the detection and typing of the human herpesviruses with known sequences and no amplification of human DNA, in spite of the presence of the same consensus regions within human DNA polymerase alpha. (nih.gov)
  • Diagnostic value of digital droplet polymerase chain reaction and digital multiplexed detection of single-nucleotide variants in pancreatic cytology specimens collected by EUS-guided FNA. (nih.gov)
  • Screening for steroid sulfatase (STS) gene deletions by multiplex DNA amplification" (PDF). (wikipedia.org)
  • This process amplifies DNA in samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler. (wikipedia.org)
  • We developed a single multiplex PCR reaction to detect ETEC, EPEC, EIEC, and STEC, using specific previously described ( 4 - 6 ) or new primers (GIBCO-BRL, Gaithersburg, MD) for diverse virulence traits ( Table 1 ). (cdc.gov)
  • The specific fragments obtained were the substrate for a second, multiplex reaction for which primers were designed to produce different-size fragments for each related virus. (nih.gov)
  • Now enter our superstar molecule-Taq Polymerase-a heat-stable enzyme obtained from bacteria living in hot springs that synthesizes ("builds") new strands of DNA using nucleotides, starting at those primers. (aboutdarwin.com)
  • The DNA polymerase is an enzyme, which is used for in vitro DNA replication. (brighthub.com)
  • The original DNA molecule is replicated by DNA polymerase enzyme and it doubles the number of DNA molecules. (brighthub.com)
  • At this temperature, the DNA polymerase enzyme works well and extends the chain further. (brighthub.com)
  • Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction). (wikipedia.org)
  • Multiplex-PCR consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. (wikipedia.org)
  • Multiplex-PCR was first described in 1988 as a method to detect deletions in the dystrophin gene. (wikipedia.org)
  • Cite this: Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli - Medscape - Jan 01, 2003. (medscape.com)
  • The use of molecular methods especially real-time multiplex PCR allowed to detect a wide range of respiratory viruses and their subtype as well as some atypical bacteria. (hindawi.com)
  • The ability of two-band and three-band multiplex polymerase chain reactions to detect and differentiate Mycobacterium tuberculosis complex from non-tuberculous mycobacteria was evaluated. (who.int)
  • Polymerase chain reaction (PCR) products of each locus. (cdc.gov)
  • Current methodologies for the analysis of the killer-cell immunoglobulin-like receptor (KIR) locus utilize specific primer-directed polymerase chain reaction (SSP-PCR), which require a wide range of DNA input, multiple reaction conditions, and up to 16 individual reactions. (elsevierpure.com)
  • We have developed and validated a multiplex SSP-PCR method for the genetic analysis of the KIR locus. (elsevierpure.com)
  • Product portfolios focus on multiplex diagnostics for sexually transmitted infection (STI), antibiotic resistance markers, and respiratory disease. (businesswire.com)
  • PCR or the Polymerase Chain Reaction is a technique, which is widely used in various scientific fields including molecular biology, diagnostics, genetics, forensic science, and paternity testing among others. (brighthub.com)
  • The test was developed by BioFire® [ 4 ] Diagnostics (Salt Lake City, Utah) and is a qualitative multiplex polymerase chain reaction (PCR) test used on the Film Array system. (biomedcentral.com)
  • The aim of this study was to investigate the epidemiology of respiratory pathogens detected in children admitted with SARI and to highlight the role of real-time multiplex PCR in the rapid diagnosis of viral and bacterial SARI. (hindawi.com)
  • The virus can be detected by polymerase chain reaction, usually using a multiplex panel of common respiratory pathogens. (msdmanuals.com)
  • The effects of a range of plasticizers and related compounds commonly encountered in the environment were evaluated kinetically against human DHEA sulfotransferase (SULT 2A1) and by reverse transcriptase-polymerase chain reaction (RT-PCR) against several enzymes involved in the synthesis of the sulfotransferase cofactor adenosine 3′-phosphate 5′-phosphosulfate (PAPS). (nih.gov)
  • This somewhat modified technique is known as Reverse transcriptase polymerase chain reaction, or RT-PCR. (brighthub.com)
  • The specificity of multiplex O1/O139 PCR was investigated by analysing 120 strains of V. cholerae, Vibrio and other Bacteria species. (umd.edu)
  • The inclusion of reactions and conditions that apply to a variety of virulence loci so that multiple candidate pathogens can be sought in a single reaction makes this technology more efficient and economical. (cdc.gov)
  • We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga-toxin-producing Escherichia coli . (cdc.gov)
  • The test of employing Multiplex PCR technology is conducted in a number of targeted gene testing at the same time. (scirp.org)
  • Current methods to authenticate these animal materials in food chain are based on mainly single gene target which are generally longer in length and thus breakdown during food processing treatments. (um.edu.my)
  • Identification of bacterial species was performed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry and pneumococci were serotyped using multiplex polymerase chain reactions. (springer.com)
  • Between May and October 2015 we analysed the serum samples of 140 patients with a suspicion of dengue, using ELISA and multiplex polymerase chain reaction. (who.int)
  • Les échantillons de sérum de 140 patients susceptibles d'avoir contracté le virus de la dengue ont été analysés entre mai et octobre 2015 à l'aide du test ELISA et de l'amplification en chaîne par polymérase multiplexe. (who.int)
  • The goal of this activity is for learners to be better able to incorporate multiplex polymerase chain reaction (PCR) testing into the comprehensive management of patients with infectious diseases to improve antibiotic stewardship practices. (medscape.org)
  • There were no cross-reactions among any of the probes. (aku.edu)
  • The machine heats and cools the test tubes present in the reaction mixture. (brighthub.com)
  • PV infection was assessed in eyebrow hair by using polymerase chain reaction-based methods. (uni-koeln.de)
  • Commercial soybean, genetically modified soy bean and oil were detected with the Multiplex PCR. (scirp.org)
  • Multiplex PCR technology is based upon the tradition PCR technology. (scirp.org)
  • Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes, i.e., their base pair length, should be different enough to form distinct bands when visualized by gel electrophoresis. (wikipedia.org)
  • Each multiplex group contains PCR products that differ in size by a minimum of 15 bp to allow sufficient fragment length resolution for size discrimination by gel electrophoresis. (elsevierpure.com)
  • These cycles are called chain reactions and millions of copies of DNA are produced from a single piece of DNA. (brighthub.com)
  • Multiplexing is often used to convert multiple analog or digital input signals through common transmission lines to output. (openwetware.org)
  • We describe here a method for DNA extraction from coastal water and a multiplex polymerase chain reaction (PCR) for O1 and O139 serogroups. (umd.edu)
  • In 2008, multiplex-PCR was used for analysis of microsatellites and SNPs. (wikipedia.org)
  • Each pair of multiplexing lines are arranged in the form a bit with an alternating pattern of thicker and thinner parts as shown in the figure. (openwetware.org)
  • A valve multiplexing system similar to electronic integrated circuits is developed to allow complex fluidic functions with a minimal number of inputs. (openwetware.org)