• However, common approaches for counteracting matrix effects (unspecific QuEChERS clean-up, standard addition, matrix-matched standard addition, SIDA [stable isotope dilution assay]) are not feasible for LC-MS-MS methods covering more than 200 mycotoxins. (chromatographyonline.com)
  • For assessing the folate content in a range of Brazilian PANCs, we extended the validation of an existing stable isotope dilution assay (SIDA) for the stably 13 C-labelled 10-formyl-Pte[ 13 C 5 ]Glu (10-CHO-Pte[ 13 C 5 ]Glu). (frontiersin.org)
  • The solution containing the chloropropyl esters was concentrated and then analyzed using gas chromatography-positive chemical ionization-tandem mass spectrometry. (cdc.gov)
  • Zhang Y, Wang Q, Zhang G, Jia W, Ren Y, Wu Y. Biomarker analysis of hemoglobin adducts of acrylamide and glycidamide enantiomers for mid-term internal exposure assessment by isotope dilution ultra-high-performance liquid chromatography tandem mass spectrometry. (cdc.gov)
  • Isobaric mass labeling leads to isobaric analytes that can be differentiated by isotopic mass reporters released from the analyte during tandem mass spec. (genengnews.com)
  • The Tandem Mass Tag® (TMT®) core structure invented by Proteome Sciences allows for the generation of different sets of isotopic and isobaric mass tags. (genengnews.com)
  • This IDMS method utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to analyze sample tryptic digests for detection and quantification of selected conserved peptides of SARS-CoV-2 spike and nucleocapsid proteins. (cdc.gov)
  • In the mouse bioassay, when the injected matography-tandem mass spectrometry method for quan- dose is high, mice typically develop signs of botulism with- tifying active toxin, where the amount of toxin can be in 8 hours. (cdc.gov)
  • It also requires several days to determine the toxin ionization-tandem mass spectrometry (HPLC- level and type (4-6). (cdc.gov)
  • A liquid chromatography tandem mass spectrometry (LC-MS-MS) "dilute and shoot" method for the determination of 331 (toxic) secondary metabolites of fungi and bacteria has recently been optimized and validated for different food matrices (1) according to the guidelines established in the Directorate General for Health and Consumer Affairs of the European Commission (SANCO) document No. 12495/2011. (chromatographyonline.com)
  • The method was established based on the stable isotope-dilution liquid chromatography-tandem mass spectroscopy (ID-LC-MS/MS) technique. (biomed.news)
  • By using our newly developed liquid chromatography-tandem mass spectrometry quantification method, we were able to measure the absolute amount of MRP2/Mrp2 in liver tissues and isolated hepatocytes across species. (aspetjournals.org)
  • The method can be applied for the analyses of target phytohormones in small tissue samples obtained from any plant species and/or plant part relying on any commercially available (even less sensitive) tandem mass spectrometry instrumentation. (biomedcentral.com)
  • A targeted ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established and validated for the simultaneous determination of 34 amino acids in tissue samples from colorectal cancer (CRC) patients. (sagepub.com)
  • To develop a reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for therapeutic drug monitoring (TDM) of unbound ciprofloxacin concentrations in critically ill patients. (betasciencepress-publishing.com)
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly utilized in clinical laboratories because it has advantages in terms of specificity and sensitivity over other analytical technologies. (annlabmed.org)
  • In this study, we tested the LTD-IDMS assay on A(H5N1) vaccine material that had been stressed by low pH, heat, and multiple freeze-thaw cycles. (cdc.gov)
  • Current methods for detecting BoNT include a mouse cause human botulism (A, B, E, and F). We developed a bioassay (2-4) and an enzyme-linked immunosorbent assay rapid, mass spectrometry-based method (Endopep-MS) to (ELISA) (5,6). (cdc.gov)
  • However, because of the unknown binding affinity of antibody to MRP2/Mrp2 protein across species and lack of purified MRP2/Mrp2 proteins for calibration, the immunoblotting assay was excluded from the absolute quantification of MRP2/Mrp2 protein for multiple species. (aspetjournals.org)
  • Full validation of the above assays, including intra-assay/inter-assay variability, spike recovery, dilution linearity, and limit of detection, is not usually reported. (jneurosci.org)
  • The DAP metabolites were quantified using isotope- dilution calibration. (cdc.gov)
  • The 13C distribution in intracellular metabolites can be detected via mass spectrometry and used as a constraint in intracellular metabolic flux calculations. (biomed.news)
  • Phytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. (biomedcentral.com)
  • Usually synthetic or recombinant proteins are used as isotopic standards and spiked into samples to serve as internal standards for quantification. (genengnews.com)
  • For both modes, absolute quantification is achieved by spiking in the reference in known amounts. (genengnews.com)
  • We present an isotope dilution mass spectrometry method (IDMS) for rapid and accurate quantification of the primary antigens, spike and nucleocapsid proteins. (cdc.gov)
  • Absolute quantification was achieved by quantifying and averaging 5 peptides for spike protein (3 peptides in the S1 subunit and 2 peptides in the S2 subunit) and 4 peptides for nucleocapsid protein. (cdc.gov)
  • 10 fmol/L) and precision for quantification of SARS-CoV-2 spike and nucleocapsid proteins. (cdc.gov)
  • Osmium isotope stratigraphy suggests a most plausible depositional age of ~11 Ma (Miocene) for this layer, as determined by fitting with the seawater evolution curve. (nature.com)
  • b ) Depositional age determined by Os isotope stratigraphy. (nature.com)
  • Instead, we used Os isotope stratigraphy to constrain the depositional age of the core samples. (nature.com)
  • Although the precise chemical and physical factors involved in ion suppression are not fully understood, it has been proposed that basicity, high concentration, mass and more intuitively, co-elution with the analyte of interest are factors which should not be ignored. (wikipedia.org)
  • Isotopic mass labeling leads to a distinct mass difference of structurally identical reference and target analyte. (genengnews.com)
  • Good linearity (R 2 ≥ 0.9887) and good repeatability (RSDs ≤ 10.35%, n = 5) for 16 analytes in spiked standard analyte samples were achieved. (biomedcentral.com)
  • Mixtures of neutralizing anti- detection of the unique peptide products by mass spec- bodies are given to mice in conjunction with the sample. (cdc.gov)
  • The minor isotopes 47 Ti (7.44% abundance) and 49 Ti (5.41% abundance) are therefore typically used for Ti quantitation by ICP-MS, so sensitivity and detection limits are poorer than they would be if a major isotope was available. (spectroscopyonline.com)
  • Conversely, chemical labeling, attaching small tags that carry isotope differences to the analytes, presents a simpler approach without the need to actually synthesize any peptides. (genengnews.com)
  • The product peptides derived from the endopeptidase injection containing a sample with more than a minimum activities of BoNTs are detected by matrix-assisted laser- lethal dose show symptoms of botulinum intoxication and desorption ionization time-of-flight mass spectrometry. (cdc.gov)
  • Mice receiving the appropriate anti-BoNT serotype anti- a unique cleavage site on a unique peptide, the mass-spe- body are asymptomatic and survive, while mice treated cific product peptides detected by mass spectrometry dif- with the other serotype antibodies show symptoms of botu- ferentiate active BoNT serotypes. (cdc.gov)
  • The main disadvantage of flight mass spectrometry (MALDI-TOF-MS) or by high- the mouse bioassay is that it requires euthanizing many ani- performance liquid chromatography (HPLC)-electrospray mals. (cdc.gov)
  • The concentrations of 20 urinary metals were determined using inductively coupled plasma mass spectrometer. (bvsalud.org)
  • Stable isotope 13C labeling is an important tool to analyze cellular metabolic flux. (biomed.news)
  • Therefore, it is essential to correct the mass spectrometry data before performing metabolic flux calculations. (biomed.news)
  • This continuum goes from preconception to fertilization to implantation through multiple critical as well as sensitive stages of differentiation, development, and growth in pregnancy followed by birth, breastfeeding, and postnatal development. (canadiancovidcarealliance.org)
  • Besides this relatively time-consuming step, even the modern GC-MS approaches still require at least 300 mg fresh plant material to reach dynamic range of a mass spectrometer [ 11 ]. (biomedcentral.com)
  • The overall relative standard deviation of the method was 3.67% for spike protein and 5.11% for nucleocapsid protein. (cdc.gov)
  • The anabolic effects of Dianabol increase muscle mass and physical strength by promoting testosterone production and protein synthesis. (shida-thaimassage.de)
  • Gas chromatography-mass spectrometry (GC-MS) is typically employed to analyze volatile components in flavorings. (biomedcentral.com)
  • However, only 28 distal impact ejecta or spherule layers have been confirmed from the Earth's surface 3 from the presence of shocked quartz, Ni-rich spinel, anomalous PGE enrichments or negative excursions of Os isotope composition. (nature.com)
  • Briefly, 4 mL of urine were spiked with an isotopically-labeled internal standard mixture, then concentrated to dryness using an azeotropic codistillation with acetonitrile or lyophilization. (cdc.gov)
  • QCM), and the third pool (QCH) were spiked with the native standard stock solution to yield concentrations of 5 ng/ml, 15 ng/ml and 30 ng/ml, respectively. (cdc.gov)
  • This clay layer has high platinum group element (PGE) concentrations and features a conspicuous negative Os isotope anomaly ( 187 Os/ 188 Os as low as ~0.2), indicating an influx of extraterrestrial material. (nature.com)
  • Below, we report bulk major- and trace-element compositions, Re-Os isotope data and PGE concentrations in the piston core samples, as well as detailed petrographic observations of this layer, that collectively indicate the presence of extraterrestrial material. (nature.com)
  • a ) Re and Os concentrations and isotope ratios. (nature.com)
  • In general, two different methods of chemical labeling are used-isotopic mass labeling and isobaric mass labeling. (genengnews.com)
  • Reaction gas methods can use either on-mass or mass-shift measurement. (spectroscopyonline.com)
  • This makes the isotopic information of elements other than the 13C marker interspersed in the isotopic distribution measured by the mass spectrometry, especially that of the molecules containing many other elements, which leads to large errors. (biomed.news)
  • Steam distillation/drop-by-drop extraction with gas chromatography-mass spectrometry for fast determination of volatile components in jujube (Ziziphus jujuba Mill. (biomedcentral.com)
  • A method coupling steam distillation/drop-by-drop extraction with gas chromatography-mass spectrometry (S3DE/GC-MS) was developed to determine the volatile components of jujube extract. (biomedcentral.com)
  • Gas chromatography (GC) coupled on-line to mass spectrometry (MS) is routinely applied for the simultaneous analysis of multiple hormones in plant tissues [ 10 ]. (biomedcentral.com)
  • The content knowledge of LC-MS/MS users is quite broad and deep and spans multiple scientific fields, including biology, clinical chemistry, chromatography, engineering, and MS. However, there are no formal academic programs or specific literature to train laboratory staff on the fundamentals of LC-MS/MS beyond the reports on method development. (annlabmed.org)
  • Currently standards must be generated one by one by incorporating isotope-labeled amino acids into the peptide sequence by chemical synthesis or by addition to culture media. (genengnews.com)
  • Multiple lines of evidence suggest that Aβ oligomers are more toxic than other peptide forms. (jneurosci.org)
  • In this paper, we proposed a method for construction of correction matrix based on Python language for correcting the measurement errors due to natural isotope distribution. (biomed.news)
  • CoVs have four main structural proteins: spike(S), membrane (M), envelope (E), and nucleocapsid (N) proteins. (medchemexpress.com)
  • As a consequence, in the hands of inexperienced groups data generation based on the improved resolving power of mass spectrometers continues to outperform the ability of many users for data interpretation. (genengnews.com)
  • e multiple reaction monitoring mode was chosen with an electrospray ionization source operating in the positive ionization mode for data acquisition. (sagepub.com)
  • Quadrupole inductively coupled plasma-mass spectrometry (ICP-QMS) is a standard technique for analyzing trace metals in many sample types including environmental, food, and biological materials. (spectroscopyonline.com)
  • This contribution presents a new reference material, ASH-15, a flowstone that is dated here by isotope dilution (ID) thermal ionization mass spectrometry (TIMS) analysis using 37 sub-samples, 1-7 mg each. (copernicus.org)
  • The range of isotope ratios in these samples is 0.781-16.9 for 187 Re/ 188 Os and 0.2274-0.927 for 187 Os/ 188 Os. (nature.com)
  • In sample matrices that contain high levels of Ca, P, C, Cl, or S, common matrix elements in many natural materials, all of the Ti isotopes can also be affected by polyatomic interferences including CaH + , PO + , SOH + , and CCl + (2). (spectroscopyonline.com)
  • Hielscher J, Monien BH, Abraham K, Jessel S, Seidel A, Lampen A. An isotope-dilution UPLC-MS/MS technique for the human biomonitoring of the internal exposure to glycidol via a valine adduct at the N-terminus of hemoglobin. (cdc.gov)
  • Here, we present petrographic and geochemical evidence of a possible impact ejecta layer, dating from about 11 Ma, in deep-sea clayey sediment in the Northwest Pacific. (nature.com)
  • We here present petrographic and geochemical evidence of a possible impact ejecta layer produced by a middle or late Miocene impact event at around 11 Ma. (nature.com)
  • However, in addition to carbon, other elements such as oxygen in the nature also have natural stable isotopes (e.g., 17O, 18O). (biomed.news)
  • To address this time lag, the isotope dilution mass spectrometry (IDMS) method was developed to quantify the absolute hemagglutinin (HA, the main influenza antigen) amount in the vaccine without the need for purified, inactivated, and calibrated virus reference antigens. (cdc.gov)
  • A conspicuous negative Os isotope excursion and an abnormal enrichment of Os were found in a ~40-cm-thick layer of clay ~350 cm below the seafloor (cmbsf) in piston core 11 (PC11). (nature.com)
  • In this study, PMBL was defined as patients with a dominant mass within the anterior mediastinum, irrespective of the tumor size. (angiogenesis-blog.com)
  • Specifically, this study investigates the temperature-driven seasonal oscillation of ACE to assess multiple sources of wastewater inputs at a riverbank filtration site. (univie.ac.at)
  • For large-scale and long-term metabolomics studies that involve a large batch or multiple batches of analyses, batch effects cause nonbiological systematic biases that may lead to false positive or false negative findings. (biomed.news)
  • Age results presented here are slightly younger compared to previous ID isotope ratio mass spectrometry (IRMS) U−Pb dates of ASH-15 but within uncertainties and in agreement with in situ analyses using WC1 as the primary RM. (copernicus.org)