• Recently, amplified fragment length polymorphism (AFLP) analysis was applied as a new method for the typing of VREF ( 1 , 33 ). (asm.org)
  • This is due to the amount of data generated from nucleotide sequencing and the information generated from an array of analytical tools improves our understanding of bacterial pathogens. (nih.gov)
  • These tools will facilitate bacterial typing based on draft genomes of multidrug-resistant Enterobacteriaceae species by the rapid detection of known plasmid types. (asm.org)
  • In conclusion, testing of the two Web tools using both fully assembled plasmid sequences and WGS-generated draft genomes showed them to be able to detect a broad variety of plasmids that are often associated with antimicrobial resistance in clinically relevant bacterial pathogens. (asm.org)
  • In infants and young children, H. influenzae type b (Hib) causes bacteremia, pneumonia, epiglottitis and acute bacterial meningitis. (wikipedia.org)
  • However, these methods, although generally superior to serotyping and phage typing, also vary in their reproducibilities and discriminatory abilities. (asm.org)
  • Because of the importance of Salmonella in food-borne disease, numerous typing methodologies have been developed and have been used to trace salmonellosis outbreaks to the contaminated source and to delineate the epidemiology of Salmonella infections. (asm.org)
  • ClonalFrame is a model-based method for using multilocus sequence data to infer the clonal relationships of bacteria and the chromosomal position of homologous recombination events that disrupt a clonal pattern of inheritance. (pubmedcentralcanada.ca)
  • Such features aid the successful spread of different plasmid types among bacteria of different sources and geographical origins. (asm.org)
  • The different sequences at each locus are assigned different allele numbers, and each strain is defined by the alleles at the seven loci (the allelic profile). (asm.org)
  • Numerous computer-based statistical packages have been developed in recent years and it has become easier to analyze nucleotide sequence data and gather subsequent information that would not normally be available. (nih.gov)
  • AFLP typing also disclosed two different human-associated lineages. (asm.org)
  • First, the annealing temperature of the PCR amplification was reduced to 47°C. Second, one of the oligonucleotide primers used to amplify the glyA locus (glyA2) was replaced with glyS4, 5′-AGGTGATTATCCGTTCCATCGC-3′, derived from the C. jejuni sequence. (pubmedcentralcanada.ca)
  • A huge set of data is produced during the sequencing and identification process so bioinformatic techniques are used to arrange, manage, analyze and merge all of the biological data. (wikipedia.org)
  • The method presented here enables investigators to determine the sequence types of their isolates on the basis of WGS data. (asm.org)
  • To strike the balance between the acceptable identification power, time and cost for the strain typing, about seven to eight house-keeping genes are commonly used in the laboratories. (wikipedia.org)
  • A series of profiles can then be the identification marker for strain typing. (wikipedia.org)
  • Multilocus Sequence Typing of Pseudomonas syringae Sensu Lato Confirms Previously Described Genomospecies and Permits Rapid Identification of P. syringae pv. (wikipedia.org)
  • Notwithstanding, proper identification remains difficult due to the complexity involved in the sequencing process as well as the relatively high costs associated with these advanced techniques. (wikipedia.org)
  • This means that advocates of phylogenetic nomenclature have removed a large number of basal Devonian and Carboniferous amphibian-type tetrapod groups that were formerly placed in Amphibia in Linnaean taxonomy, and included them elsewhere under cladistic taxonomy. (wikipedia.org)
  • Streptomyces is the largest genus of the Actinobacteria and is the type genus of the family Streptomycetaceae. (wikipedia.org)
  • Detection of sequences highly similar to B. bissettii strain DN127 in cardiac valve tissue of a patient with endocarditis and aortic valve stenosis [ 8 ], and in serum samples of patients in the Czech Republic [ 9 ], raised concern about the pathogenic potential of B. bissettii . (biomedcentral.com)
  • By knowing the epitope sequence of the antibody, ELISA can also be used for antigen detection in a sample. (wikipedia.org)
  • The class comprises one order, the Hydrogenophilales (type order), which contains thermophilic organisms - both autotrophs and heterotrophs, the former of which utilise molecular hydrogen as their electron donor, coupling its oxidation to the reduction of NAD+ with the enzyme hydrogenase. (wikipedia.org)
  • The four orders of the Betaproteobactera are sub-divided into families: Burkholderiales (type order) comprises the families Burkholderiacae (type family), Alcaliginaceae, Commamonadaceae, Oxalobacteraceae and Stutterellaceae. (wikipedia.org)
  • Both heterotrophs and photoheterotrophs are found along with some facultative autotrophs.Neisseriales comprises the families Neisseriaceae (type family) and Chromobacteriaceae. (wikipedia.org)
  • Most organisms are heterotrophs with some facultative methylotrophs and chemolithoheterotrophs.Nitrosomonadales comprises the families Nitrosomonadaceae (type family), Methylophilacae, Thiobacillaceae, Sterolibacteriacae, Spirillaceae and Gallionellaceae. (wikipedia.org)
  • Most organisms are chemolithoautotrophs with some methylotrophs and heterotrophsRhodocyclales comprises the families Rhodocyclaceae (type family), Azonexaceae and Zoogloeaceae. (wikipedia.org)