• The first part of this thesis focuses on the interactions of the Ski proteins with ribosomes in the exosome-dependent 3'-to-5' mRNA degradation pathway. (uni-muenchen.de)
  • The insulin-like growth factor binding proteins (IGFBPs) comprise a family of six distinct proteins which modulate insulin-like growth factor action. (eur.nl)
  • Once bound to the mRNA HuR is recognized by adapter proteins which then facilitate nuclear export of the complex. (uncg.edu)
  • I wish to find an order of magnitude for the binding/unbinding constants of mRNA and RNA-binding proteins. (stackexchange.com)
  • The researches studied two RNA binding proteins: HuD and U1A, as well as several mutants. (stackexchange.com)
  • As a means to identify cellular RNA targets for these proteins, we devised combinatorial shape libraries representing naturally derived 3' untranslated regions and were able to select a structurally related subset of transcripts that bound to Hel-N1. (duke.edu)
  • Approximately 10% of the proteins encoded by these subset mRNAs were identifiable in the data bases and most are implicated in cell growth regulation. (duke.edu)
  • This approach provides a means to gain access to novel genes expressed in various cell types by partitioning mRNAs containing common sequence elements using RNA-binding proteins. (duke.edu)
  • RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
  • Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
  • RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
  • Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
  • c ) Overlap of mRNA interactome proteins in yeast and HuH-7. (nature.com)
  • d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
  • In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
  • These data suggest that the 243- and 289-amino acid E1A proteins both interact functionally with the cAMP signaling system to activate transcription of a cellular gene and AP-1-binding activity. (princeton.edu)
  • Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. (elsevierpure.com)
  • Here, we show that the RNA binding proteins TIA1 and TIAL1 are required for the generation of long-lasting GC responses. (ox.ac.uk)
  • RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
  • Together these proteins shorten the end of the mRNAs, preparing the molecules for degradation. (elifesciences.org)
  • Phytochrome Regulation of Greening in Pisum: Chlorophyll Accumulation and Abundance of mRNA for the Light-Harvesting Chlorophyll a/b Binding Proteins. (ncsu.edu)
  • We compared time courses, escape from reversal by far-red light, and fluence-response behavior for induction of mRNA for the light-harvesting chlorophyll a/b binding proteins (Cab mRNA) with those for induction of rapid chlorophyll accumulation in seedlings of Pisum sativum cv Alaska. (ncsu.edu)
  • For example, exosomes and nanovesicles secreted by living cells serve as intercellular couriers of mRNA, microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and proteins and have thus garnered considerable interest [ 3 ]. (jcancer.org)
  • Translational operator of mRNA on the ribosome: how repressor proteins exclude ribosome binding. (igbmc.fr)
  • RNA binding proteins recognize these sequences and assemble with their mRNA target into mRNPs. (europa.eu)
  • It is still not known which RBPs or adaptor proteins are essential to recruit microtubule-binding motor proteins to mRNPs and to which extend this varies between different transported mRNAs. (europa.eu)
  • Mutations affecting zipcodes, RBPs or motor-proteins required for neuronal mRNA localization were shown to lead to severe neurodegenerative diseases as ALS, FXTAS and FXS (7), underlining the need to understand the mechanisms that drive neuronal mRNA transport. (europa.eu)
  • Also in this specific case, it is not understood how RBPs, potential adaptors and motor proteins, which are essential for the transport of CaMKIIa-mRNA are mechanistically contributing to its correct localisation. (europa.eu)
  • Once free, the mRNA is translated by ribosomes to make proteins (left). (acs.org)
  • The crucial ingredient in each injection is mRNA, short-lived strands of genetic material that prompt our cells to start making SARS-CoV-2 proteins, which in turn help our immune systems develop antibodies that prevent future infections. (acs.org)
  • Some studies indicate that matrin 3 binds and stabilizes a type of RNA called messenger RNA (mRNA), which provides the genetic blueprint for proteins. (medlineplus.gov)
  • Matrin 3 may also bind certain abnormal RNAs that could lead to nonfunctional or harmful proteins, thereby blocking the formation of such proteins. (medlineplus.gov)
  • They are RNA-BINDING PROTEINS involved in mRNA processing. (bvsalud.org)
  • T3, the proposed active form (from cell cytoplasm), and T3 and T4 (generated in situ) diffuse into the nucleus and bind to specific thyroid receptor proteins, which appear to be attached primarily to DNA. (medscape.com)
  • Receptor binding leads to the activation or repression of DNA transcription, altering the amounts of mRNA and resultant proteins. (medscape.com)
  • A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. (nih.gov)
  • The relationship between NAFLD and retinol-binding protein 4 - an updated systematic review and meta-analysis. (nih.gov)
  • Positive Expression of Retinol-Binding Protein 4 Is Related to the Malignant Clinical Features Leading to Poor Prognosis of Glioblastoma. (nih.gov)
  • Interplay of retinol binding protein 4 with obesity and associated chronic alterations (Review). (nih.gov)
  • The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. (nih.gov)
  • ATP-Binding Cassette Transporter Family C Protein 10 Participates in the Synthesis and Efflux of Hexosylceramides in Liver Cells. (nih.gov)
  • Scholars@Duke publication: Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. (duke.edu)
  • Hel-N1, a human RNA-binding protein, shares significant homology with Drosophila protein ELAV, which is essential for fly neuronal development. (duke.edu)
  • The myosin-binding protein C, cardiac-type is a protein that in humans is encoded by the MYBPC3 gene. (wikipedia.org)
  • cMyBP-C is a myosin-associated protein that binds at 43 nm intervals along the myosin thick filament backbone, stretching for 200 nm on either side of the M-line within the crossbridge-bearing zone (C-region) of the A band in striated muscle. (wikipedia.org)
  • In its dephosphorylated state, cMyBP-C binds predominantly to myosin S2 and brakes crossbridge formation, however, when phosphorylated in response to β-adrenergic stimulation through activating cAMP-dependent protein kinase (PKA), it favours binding to actin, then accelerating crossbridge formation, enhancing force development and promoting relaxation. (wikipedia.org)
  • Furthermore, GSK3β was described as another protein kinase to phosphorylate cMyBP-C outside the M-domain in the proline-alanine-rich actin-binding site at Ser133 in human myocardium (mouse Ser131). (wikipedia.org)
  • In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization. (ox.ac.uk)
  • In situ hybridization is used to survey the tissue-specific and developmental expression of the cloned mouse gene Sparc, coding for a protein homologous to the bovine Ca++-binding protein, osteonectin. (ox.ac.uk)
  • Here, we present evidence that p21 expression increases through PGA 2 -triggered stabilization of the p21 mRNA and further show that these events require the mitogen-activated protein (MAP) kinase ERK. (johnshopkins.edu)
  • Binding experiments using either endogenous p21 mRNA or in vitro-labeled p21 transcripts revealed a specific PGA 2 -dependent association of the p21 mRNA with the RNA-binding protein HuR. (johnshopkins.edu)
  • In this work, a novel U-rich RNA-binding protein (TcUBP-1) from Trypanosoma cruzi, the agent of Chagas disease, was identified. (elsevierpure.com)
  • Because TcUBP-1 is developmentally regulated, it might have a relevant role in regulating protein expression during trypanosome differentiation, allowing a correct expression pattern of U-rich-containing mRNAs. (elsevierpure.com)
  • D'Orso, I & Frasch, ACC 2001, ' TcUBP-1, a Developmentally Regulated U-rich RNA-binding Protein Involved in Selective mRNA Destabilization in Trypanosomes ', Journal of Biological Chemistry , vol. 276, no. 37, pp. 34801-34809. (elsevierpure.com)
  • Significant E1A-dependent induction of c-fos mRNA and AP-1-binding activity was observed in cells expressing either E1A protein. (princeton.edu)
  • Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. (elsevierpure.com)
  • LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. (elsevierpure.com)
  • eIF4E the mRNA 5′ cap-binding protein is governed by its binding protein (4E-BP) a downstream target of phosphatidylinositol-3-OH kinase [PI(3)K] signaling. (bioxorio.com)
  • When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
  • The more mRNA copies it makes, the more protein it can produce. (elifesciences.org)
  • A simple way to control protein production is to raise or lower the number of these mRNA messages, and living cells have lots of ways to make this happen. (elifesciences.org)
  • The test-tube system confirmed previous suggestions that a protein called Puf3 forms a bridge between Ccr4-Not and mRNAs. (elifesciences.org)
  • mRNA steady state levels of the chlorophyll a/b-binding protein of photosystem II, ferredoxin I, the small and large subunits of ribulose 1,5-bisphosphate carboxylase, and pEA214 and pEA207, two other light-responsive genes, were determined during chlorophyll photooxidation. (ncsu.edu)
  • Analyses of chlorophylls a and b and the chlorophyll a/b-binding protein suggest that accumulation of the protein and its mRNA are coordinated with chlorophyll abundance at several steps. (ncsu.edu)
  • In addition to post-transcriptional regulation at the level of mRNA and protein stability, there may exist coordination at the transcriptional stage. (ncsu.edu)
  • Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. (lu.se)
  • Inhibiting the localisation of this mRNA leads to a significant reduction of CamKIIa protein at postsynaptic densities and a strong reduction of cognitive abilities in animal models (12). (europa.eu)
  • The effects of dermatotoxic chemicals on activator protein (AP1) DNA binding activity and c-jun and c-fos messenger RNA (mRNA) levels were examined in normal human epidermal keratinocytes. (cdc.gov)
  • This protein can attach to (bind) RNA, which is a chemical cousin of DNA. (medlineplus.gov)
  • DNA-binding protein RFX5 (Regulatory factor X subunit 5). (lu.se)
  • A neuron-specific in expression RNA-BINDING PROTEIN, involved in neurological disorders and cancer. (bvsalud.org)
  • Context and state of the art mRNA transport in general: Cytoplasmic mRNA transport and local translation are essential for many processes requiring symmetry breaking such as embryonic development, cell migration and neuronal differentiation (1). (europa.eu)
  • Since the first discovery of cytoplasmic mRNA transport almost three decades ago, several essential questions could not be answered mostly due to the complexity of the in vivo situation and the approaches available. (europa.eu)
  • Independent clinical trials demonstrated 94-95% vaccine efficacy against symptomatic disease caused by SARS-CoV-2 for both the Pfizer/BioNTech and Moderna mRNA-based vaccines 3 , 4 . (nature.com)
  • With the success of COVID-19 vaccines, newly created mRNA vaccines against other infectious diseases are beginning to emerge. (mdpi.com)
  • The unprecedently speedy development of mRNA vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was enabled with previous innovations in nucleoside modifications during in vitro transcription and lipid nanoparticle delivery materials of mRNA. (mdpi.com)
  • Recent updates are briefly described in the status of mRNA vaccines against SARS-CoV-2, influenza virus, and other viral pathogens. (mdpi.com)
  • Messenger RNA (mRNA) emerged as a powerful therapeutic tool for treatments in gene therapy, oncology, and infectious diseases, as recently demonstrated by vaccines against Covid-19. (biaseparations.com)
  • Fragile mRNA molecules used in COVID-19 vaccines can't get into cells on their own. (acs.org)
  • There were many, many skeptics," says Frank DeRosa, who began working with mRNA in 2008 and is now chief technology officer at Translate Bio, a firm developing mRNA vaccines with Sanofi. (acs.org)
  • The mRNA COVID-19 vaccines (Moderna and Pfizer- for inclusion in this test-negative case-control assessment ( 1 , 3 ). (cdc.gov)
  • mRNA vaccines elicit potent neutralizing activity against homologous pseudovirus. (cdc.gov)
  • Using in vitro RNA gel shifts we have demonstrated that the C/EBPß message is a ligand for HuR and that the single binding site is an adenylate-uridylate rich element in the 3'untranslated region. (uncg.edu)
  • Hel-N1 has been shown to bind in vitro to 3' untranslated regions of mRNAs encoding c-myc, c-fos, granulocyte/macrophage colony-stimulating factor, and transcriptional repressor, Id. (duke.edu)
  • Here, we review the structural elements required for designing mRNA vaccine constructs for effective in vitro synthetic transcription reactions. (mdpi.com)
  • Interestingly, although inhibition of the ERK pathway did not prevent the PGA 2 -triggered increase in cytoplasmic HuR, it did impair the formation of endogenous and in vitro [HuR-p21 mRNA] complexes and further prevented the PGA 2 -mediated stabilization of the p21 mRNA, suggesting that ERK-mediated events were required for binding HuR to the p21 mRNA and preventing its decay. (johnshopkins.edu)
  • One of the greatest challenges in mRNA production is the removal of process-related impurities stemming from in vitro transcription (IVT) reaction, such as residual nucleotide triphosphates, DNA template, enzymes, abortive transcripts. (biaseparations.com)
  • From the pre-mRNA sequences returned in this screen we discovered physiologically relevant ASF recognition element motifs. (nih.gov)
  • The mRNAs can carry short sequences of genetic letters that can trigger their own destruction. (elifesciences.org)
  • Therefore, the level of Cab mRNA, as measured by the presence of sequences hybridizing to a cDNA probe, does not limit the rate of chlorophyll accumulation after transfer of pea seedlings to white light. (ncsu.edu)
  • This machinery recognizes pre-mRNA sequences at several motifs - the 5′ and 3′ splice sites, the branch point, and polypyrimidine tracts - positioned at exon-intron boundaries [ 4 ]. (biomedcentral.com)
  • mRNA localisation requires primary sequences and secondary structures often localised in their 3'UTR (3). (europa.eu)
  • The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. (elsevierpure.com)
  • A localization element that is predominantly in the 3′-untranslated region (UTR) can direct this mRNA to the bud. (elsevierpure.com)
  • We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors. (elsevierpure.com)
  • The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and translation. (elifesciences.org)
  • The Dynabeads™ mRNA Purification Kit contains magnetic beads for the isolation of the mRNA transcriptome from any total eukaryotic RNA preparation (see figure). (thermofisher.com)
  • We used integrated lncRNA and mRNA microarrays to determine the expression profiles of PEX-treated DCs and normal DCs derived from five healthy donors. (jcancer.org)
  • Our research studied the lncRNA and mRNA expression profiles of monocyte-derived DCs and demonstrated the functional networks that are involved in monocyte-derived DCs-mediated regulation in AR. (frontiersin.org)
  • The expression profiles of long noncoding RNAs (lncRNAs) and mRNAs of PEX-treated dendritic cells are unknown. (jcancer.org)
  • A coexpression network was constructed to identify the correlation between differentially expressed lncRNAs and mRNAs and further validated using real-time quantitative PCR in twenty healthy donors. (jcancer.org)
  • We identified 3,227 and 924 differentially expressed lncRNAs and mRNAs, respectively, in PEX-treated DCs. (jcancer.org)
  • To our knowledge, this is the first study to detect the differential expression of lncRNAs and mRNAs associated with PEX-treated DCs. (jcancer.org)
  • lncRNAs and mRNAs in the NP group (DCs isolated from NP) and the test group (DCs isolated from patients with AR) were identified via chip technology and bioinformatic analyses. (frontiersin.org)
  • Moreover, bioinformatic analyses were employed to identify the related biological functions of monocyte-derived DCs and construct the functional networks of lncRNAs and mRNAs that are differentially expressed (DE) in imDCs and mDCs. (frontiersin.org)
  • Quantitative RT-qPCR was used to study the significant fold changes of lncRNAs and mRNAs. (frontiersin.org)
  • ASF binds two motifs: a C-rich and a purine rich motif. (nih.gov)
  • Different mRNAs carry different destabilising motifs. (elifesciences.org)
  • What is puzzling is that the destabilising motifs that control different groups of mRNAs can be very similar, and scientists do not yet know how Ccr4-Not can tell the difference, or what triggers it to start breaking down groups of mRNAs. (elifesciences.org)
  • Stepwise sequential assembly of spliceosome components on these pre-mRNA motifs executes splicing reactions. (biomedcentral.com)
  • To address the second, we investigated RNAs bound by the metabolic enzyme hydroxysteroid dehydrogenase 17-β 10 (HSD17B10). (nature.com)
  • The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)
  • The B+/C+ genes show a significant change in mRNA expression for the 7 transcription factors for which deletion and ChIP data is available. (biomedcentral.com)
  • Products of the adenovirus E1A gene can act synergistically with cAMP to activate transcription of several viral early genes and the cellular genes c-fos and jun-B. Transcription factor AP-1-binding activity is also induced by the combined action of E1A and cAMP. (princeton.edu)
  • This allows the cell to switch networks of related genes off together without affecting the mRNAs it still needs. (elifesciences.org)
  • Nuclear and cytoplasmic mRNAs for several phytochrome-regulated genes were examined in Pisum seedlings in order to investigate possible light effects on mRNA partitioning between the nucleus and cytoplasm. (ncsu.edu)
  • Cancer genomics analysis revealed that 36 of the most differentially expressed mRNAs were involved in a pancreatic cancer network and were associated with many critical mutated genes such as TP53, KRAS, SMAD4, and CDKN2A. (jcancer.org)
  • The authors conclude that dermatotoxic chemical exposure increases the AP1 DNA binding activity and the stimulation of early/immediate genes. (cdc.gov)
  • Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
  • Over-expression of TcUBP-1 in trypanosomes decreases the half-life of SMUG mucin mRNAs in vivo but does not affect the stability of other parasite mRNAs. (elsevierpure.com)
  • mRNA constructs typically contain a 3' polyA tail to increase stability in vivo, thereby enabling affinity purification using oligo-deoxythymidinic acid (Oligo dT) probes covalently coupled to a solid support. (biaseparations.com)
  • Consequently, motif quality is a major determinant of mRNA stability for Puf3 targets in vivo and can be used for the prediction of mRNA targets. (elifesciences.org)
  • In this study, we compared immune responses to the BNT162b2 mRNA Coronavirus Disease 2019 vaccine in patients with solid tumors ( n = 53) who were on active cytotoxic anti-cancer therapy to a control cohort of participants without cancer ( n = 50). (nature.com)
  • Significant decrease in humoral and cellular responses induced by Pfizer-BioNTech vaccine BNT162b2 ( https://www.pfizer.com ) against SARS-CoV-2 from 3 weeks to 16 weeks after second vaccination, observed in a study of immune response against variants of concern in dialysis patients 4 months after SARS-CoV-2 mRNA vaccination. (cdc.gov)
  • Safety and efficacy of the BNT162b2 mRNA Covid-19 vaccine through 6 months. (cdc.gov)
  • Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. (lu.se)
  • We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure. (lu.se)
  • Intact mRNA can be obtained in approximately one hour, and eluted in small volumes. (neb.com)
  • Additionally, magnetic separation technology permits elution of intact mRNA in small volumes, thereby eliminating the need for precipitation of the poly(A)+ transcripts in the eluent. (neb.com)
  • The Dynabeads™ mRNA Purification Kit rapidly isolates the mRNA transcriptome in typically 15 minutes, delivering pure, intact mRNA. (thermofisher.com)
  • The ability to destroy specific mRNA messages is critical for cell survival. (elifesciences.org)
  • Before we can understand how overall distributions of thousands of mRNA are generated, we first need to understand how any specific mRNA can be transported along microtubules - a question unanswered since the first MT based mRNA transport was observed more than 2 decades ago. (europa.eu)
  • One problem with identifying binding sites for splicing factors arises from the transient role of RNA in gene expression. (nih.gov)
  • In addition, we studied the mRNA expression of the six mIGFBPs during development and in various adult tissues. (eur.nl)
  • In liver, a peak of mIGFBP-1 mRNA expression was found around birth, suggesting a function for mIGFBP-1 in the newborn mouse. (eur.nl)
  • The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
  • RNA interference-based knockdown of HuR abundance further served to demonstrate the contribution of HuR-mediated p21 mRNA stabilization toward enhancing p21 expression after PGA 2 treatment. (johnshopkins.edu)
  • Shortening or removal of mRNA poly(A) tails (deadenylation) represses gene expression in eukaryotes. (elifesciences.org)
  • Chen J, Wang S, Jia S, Ding G, Jiang G, Cao L. Integrated Analysis of Long Non-Coding RNA and mRNA Expression Profile in Pancreatic Cancer Derived Exosomes Treated Dendritic Cells by Microarray Analysis. (jcancer.org)
  • Notably, organ transplant recipients mount very poor antibody responses to the first mRNA immunization relative to healthy individuals 10 , which increase somewhat after the second immunization 8 . (nature.com)
  • Antibody persistence through 6 months after the second dose of mRNA-1273 vaccine for Covid-19. (cdc.gov)
  • Immunohistochemistry using antibody binding to LINE-1 open. (lu.se)
  • Available residual clinical serum specimens were collected from prior SARS-CoV-2 infection, serum antibody levels (anti-spike fully vaccinated hospitalized control patients at all sites and tested immunoglobulin G [IgG] and anti-receptor binding domain at CDC. (cdc.gov)
  • Poly(A) RNA was enriched using Dynabeads ® mRNA Purification Kit (Invitrogen), Poly(A)Purist™ MAG (Invitrogen) or the NEBNext High Input Poly(A) mRNA Magnetic Isolation Module from 50 μg Universal Human Reference RNA (UHR, Agilent) or RNA extracted from mouse kidney tissue or S. cerevisiae (yeast) using the Monarch ® Total RNA Miniprep Kit. (neb.com)
  • The Dynabeads™ mRNA Purification Kit uses a robust affinity purification principle for the enrichment of polyadenylated mRNA. (thermofisher.com)
  • mRNA translation and degradation are mutually interdependent processes in the cell. (uni-muenchen.de)
  • It was shown for these pathways that mRNA degradation is initiated in a ribosome-dependent manner directly on the stalled intermediate. (uni-muenchen.de)
  • However, no structural insights exist on how translation and degradation are coupled for general mRNA turnover and quality control. (uni-muenchen.de)
  • Collectively, these results are the first structural insights into how translation and degradation of mRNAs are coupled on a molecular level. (uni-muenchen.de)
  • Trans-acting factors binding to cis-elements that affect mRNA stability of mature transcripts have not been identified in these cells. (elsevierpure.com)
  • A video system was applied to the determination of relative changes in the mRNA transcripts. (cdc.gov)
  • Here we investigated whether d4E-BP is essential under starvation and oxidative stress conditions because dFOXO activates the transcription of and mRNA levels increase upon starvation (Zinke et al. (bioxorio.com)
  • Dermatotoxic chemical stimulate of c-jun and c-fos transcription and AP-1 DNA binding in human keratinocytes. (cdc.gov)
  • Reverse transcription and polymerase chain reaction was performed with extracted mRNA. (cdc.gov)
  • Puf3 allows Ccr4-Not to select specific mRNAs from a mixture of molecules. (elifesciences.org)
  • The kit is designed to specifically target, capture, and purify mRNA molecules from total RNA preparations. (thermofisher.com)
  • The ribosomal RNA and small RNA molecules (transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA) do not bind to the beads and are discarded. (thermofisher.com)
  • DARPA & Moderna teamed up to create mRNA gene ther. (davidicke.com)
  • The intervention that we're focused on is an additional dose of mRNA COVID-19 vaccine, an additional dose of Pfizer vaccine or Moderna vaccine after an initial two-dose primary series of an mRNA vaccine in immunocompromised people. (cdc.gov)
  • A high resolution cryo-EM structure of a native ribosome-Ski complex reveals how the Ski complex interacts with the 40S subunit of the ribosome, facilitating the threading of mRNA into the Ski2 helicase. (uni-muenchen.de)
  • A good example is the CaMKIIa-mRNA, which encodes the a-subunit of the Ca2+-calmodulin kinase II. (europa.eu)
  • In most patients with cancer, we detected spike receptor-binding domain and other S1-specific memory B cell subsets as potential predictors of anamnestic responses to additional immunizations. (nature.com)
  • Cross-neutralization of strains with receptor binding domain (RBD) mutations is poor. (cdc.gov)
  • A lipid nanoparticle (LNP) containing messenger RNA (mRNA) enters a cell through an endosome (right). (acs.org)
  • Messenger RNA (mRNA) is having a moment. (acs.org)
  • HuR is a ligand for nuclear mRNAs containing adenylate-uridylate rich elements in the 3'- untranslated region. (uncg.edu)
  • Different mRNAs are characterized by different nuclear-cytoplasmic `partitioning coefficients', indicating that post-transcriptional events play a significant role in regulating the accumulation of these mRNAs during light induction. (ncsu.edu)
  • Inhibition of mRNA deadenylation by the nuclear cap binding complex (CBC). (wikigenes.org)
  • Gene Ontology (GO), KEGG, and cancer genomics analyses were performed to identify significant functions, pathways, and the associations of differentially expressed mRNAs. (jcancer.org)
  • GO and pathway analysis revealed differentially expressed mRNAs involved in many critical biological processes and molecular functions. (jcancer.org)
  • LncRNAs such as ENST00000560647 and mRNAs such as legumain (lgmn) were differentially expressed in PEX-treated DCs, and the data were validated using RT-qPCR. (jcancer.org)
  • Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. (nature.com)
  • We also determined the RNA targets of an RNA-binding mitochondrial enzyme and show its specificity in RNA binding. (nature.com)
  • When the LNP is inside the acidic endosome (middle), the ionizable lipids become positively charged and help release the LNP and mRNA into the cell's cytoplasm. (acs.org)
  • Superparamagnetic Dynabeads™, coupled to oligo-(dT) 25 , are first equilibrated with Binding Buffer, and then mixed with purified total RNA. (thermofisher.com)
  • Kamar N , Abravanel F , Marion O , Couat C , Izopet J , Del Bello A . Three doses of an mRNA Covid-19 vaccine in solid-organ transplant recipients. (cdc.gov)
  • Matrin 3 binds and stabilizes mRNA. (mpg.de)
  • Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)
  • Pre-mRNA is a labile species with a steady state level that is three orders of magnitude less abundant than mRNA. (nih.gov)
  • This defines the extent to which transport of an mRNP is biased towards one direction, which ultimately gives rise to the steady state distributions pattern of mRNAs observed (5). (europa.eu)
  • Then, mRNA cDNA and miRNA cDNA were synthesized using miRcute miRNA first-strand cDNA kit (Tiangen Biotech) and One Step Real-Time PCR kit (Beijing Transgen Biotech) [ 11 ]. (hindawi.com)
  • After induction of long-term potentiation, CaMKIIa-mRNA is transported to distal parts of dendrites where its translation is locally regulated (12, 13). (europa.eu)
  • CPSF30 and Wdr33 directly bind to AAUAAA in mammalian mRNA 3' processing. (bvsalud.org)
  • mRNA was detectable in all brain areas examined. (nih.gov)
  • Chemical treatment increased the detectable levels of c-fos mRNA in keratinocytes. (cdc.gov)
  • Binding of human papillomavirus 16 E6 to p53 and E6AP is impaired by monoclonal antibodies directed against the second zinc-binding domain of E6. (igbmc.fr)
  • Collectively, our results indicate that PGA 2 stabilizes the p21 mRNA through an ERK-independent increase in cytoplasmic HuR levels and an ERK-dependent association of HuR with the p21 mRNA. (johnshopkins.edu)
  • We also adapted the mRNA interactome capture protocol to yeast (see Methods and Supplementary Fig. 1 ) using PAR-CL at 0.72 or 7.2 J cm −2 (ref. 8 ) with 4-thio-uracil. (nature.com)
  • Figure 1: mRNA interactome capture in yeast and HuH-7 cells. (nature.com)
  • a ) Schematic representation of the mRNA interactome capture protocol in yeast and HuH-7 cells using PAR crosslinking (PAR-CL) or conventional crosslinking (cCL). (nature.com)
  • The purpose of this study was to determine the mRNA levels in the yeast mutant rpc128-1007 and its overdose suppressors, RBS1 and PRT1. (mdpi.com)
  • Neuronal mRNA transport occurs in packages of single to a few copies of mRNAs (8-11), mostly in a translationally repressed mode. (europa.eu)
  • High densities of LGR7 mRNA hybridization were detected in deep layers of neocortex, hypothalamic paraventricular and supraoptic nuclei and within hippocampal subiculum and CA3, the basolateral amygdala and subfornical organ. (wiley.com)
  • Dendritic localisation of CaMKIIa-mRNA requires its 3'UTR harbouring binding sites for RBPs as FMRP and Staufen2, which are required for its correct localisation. (europa.eu)
  • Characterization of a chloroplast sequence-specific DNA binding factor. (ncsu.edu)
  • Extra column washes before elution may help in minimizing binding reagent carry over. (neb.com)
  • Enzymes in the environment and in our bodies are quick to chop mRNA into pieces, making lab experiments difficult and the delivery of mRNA to our cells daunting. (acs.org)
  • Genetic variation in the ATP binding cassette transporter ABCC10 is associated with neutropenia for docetaxel in Japanese lung cancer patients cohort. (nih.gov)
  • Puf3 could distinguish between mRNAs that differed in a single letter of genetic code. (elifesciences.org)
  • The NEBNext High-Input Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from high inputs (5-50 µg per 0.2 ml reaction) of previously isolated total RNA, using oligo d(T) 25 -coupled paramagnetic beads. (neb.com)
  • The NEBNext High-Input Poly(A) mRNA Magnetic Isolation Module isolates intact poly(A)+ RNA from high inputs (5-50 µg per 0.2 ml reaction) of previously isolated total RNA. (neb.com)
  • For inputs less than 5 µg, the NEBNext ® Poly(A) mRNA Magnetic Isolation Module ( NEB #E7490 ) is recommended. (neb.com)
  • Affinity-based chromatographic isolation of mRNA is robust and simple, lending itself as a useful industrial platform. (biaseparations.com)
  • These parasites make use of post-transcriptional regulatory mechanisms and modification of mRNA half-life is a relevant one. (elsevierpure.com)
  • Furthermore, we show by ribosomal profiling analysis that this interaction is probably not limited to mRNA quality control, but might rather represent a general mRNA turnover intermediate. (uni-muenchen.de)
  • There's even a lab in my department working on getting binding/unbinding rates from single-molecule microscopy data. (stackexchange.com)
  • That article has a good explanation of SPR and how it works, so I won't offer to much detail other than to say it basically measures the change of refractive index as one molecule binds to another, which is immobilized on a chip. (stackexchange.com)
  • The best characterized examples of the interplay between these two processes are the mRNA quality control pathways taking care of aberrant mRNAs that cause translation stalling in most cases. (uni-muenchen.de)
  • However within 30 min of exposure to the differentiation stimulus the HuR content in the cytosol increases consistent with HuR regulating the availability of relevant mRNAs for translation. (uncg.edu)
  • The fluence-response curve for the Cab mRNA increase, on the other hand, showed threshold and saturation at fluences 10-fold lower than threshold and saturation values for the greening response. (ncsu.edu)
  • The technology is based on the coupling of oligo d(T) 25 to paramagnetic beads, which is then used as the solid support for the direct binding of poly(A)+ RNA. (neb.com)
  • Intact poly(A)+ RNA, which is representative of the mRNA population of the original sample, can be obtained in approximately one hour. (neb.com)
  • Because poly(A) addition by Poly(A) Polymerase is distributive, the tailed mRNA is heterogeneous in length, gel purification of tailed mRNA is not recommended. (neb.com)
  • Spatially restricted but high levels of SPARC mRNA are also seen in the adult adrenal glands, testis, and ovary. (ox.ac.uk)
  • pEA207 mRNA levels, on the other hand, were resistant to the effects of photobleaching. (ncsu.edu)
  • Within 60 minutes of exposure to PMA, c-jun mRNA levels were elevated. (cdc.gov)
  • mRNA is produced by an enzymatic reaction that can be rapidly designed and scaled-up, and the platform is highly adaptable to different targets. (biaseparations.com)