• The molecular link between splicing and mRNA localization/local translation has also been demonstrated in mammalian neurons. (frontiersin.org)
  • Here, we show that the endosomal protein WDFY2 controls the recycling of MT1-MMP. (nature.com)
  • To define the localization of WDFY2 in the endocytic pathway, we transiently transfected hTERT-RPE1 cells with green flourescent protein (GFP)-WDFY2 and performed structured illumination microscopy (SIM) together with APPL1 and EEA1 visualized with antibodies (Fig. 1a ). (nature.com)
  • Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
  • The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
  • To examine the involvement of interchromatin granule clusters (IGCs) in transcription and pre-mRNA splicing in mammalian cell nuclei, the serine-arginine (SR) protein kinase cdc2-like kinase (Clk)/STY was used as a tool to manipulate IGC integrity in vivo. (rupress.org)
  • Throughout these described changes in expression, DmFKBP12 mRNA remain stable, which indicates that protein dynamics are attributed to regulation at the mRNA to protein translation level. (biomedcentral.com)
  • In the tests described in this specific article, we used gene microarray evaluation to correlate modifications in ribonucleic acidity (RNA) transcription in AVMs and CCMs towards the previously released abnormal protein appearance in these lesions. (health-e-nc.org)
  • When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
  • The more mRNA copies it makes, the more protein it can produce. (elifesciences.org)
  • A simple way to control protein production is to raise or lower the number of these mRNA messages, and living cells have lots of ways to make this happen. (elifesciences.org)
  • The test-tube system confirmed previous suggestions that a protein called Puf3 forms a bridge between Ccr4-Not and mRNAs. (elifesciences.org)
  • We demonstrate that miR-31 inhibits MCM2 expression via its 3'-untranslated region, that knockdown of MCM2 in DAOY cells leads to a degree of growth inhibition comparable to that by miR-31 restoration, and that overexpression of miR-31 reduces the chromatin loading of MCM2 at the point of G1/S transition. (oncotarget.com)
  • However, both the accumulation of splicing factors at sites of pre-mRNA synthesis as well as pre-mRNA splicing are dramatically reduced, demonstrating that IGC disassembly perturbs coordination between transcription and pre-mRNA splicing in mammalian cell nuclei. (rupress.org)
  • IGCs are composed of particles measuring 20-25 nm in diameter, and they contain numerous factors that are involved in RNA synthesis and processing. (rupress.org)
  • Fertilized egg, through the profile as under-membrane cortex distribution concentering onto basement at cellular blastoderm, to the profile as three-gem layer localization in primitive neuronal and digestion architecture of early Drosophila gastrula. (biomedcentral.com)
  • In this study, we identified both temporal and localization changes in expression of DmFKBP12, a translational and transcriptional regulator of Drosophila RyR (DmRyR) and FKBP12, through embryonic development. (biomedcentral.com)
  • RNA transport and regulated local translation play critically important roles in spatially restricting gene expression in neurons. (frontiersin.org)
  • Global gene expression profiling of mouse medulloblastomas and bioinformatics analyses of microRNA targets suggest that minichromosome maintenance complex component 2 (MCM2) is a likely target gene of miR-31 in suppressing cell growth. (oncotarget.com)
  • Shortening or removal of mRNA poly(A) tails (deadenylation) represses gene expression in eukaryotes. (elifesciences.org)
  • Research areas in Molecular Biology and Genomics, focusing on large-scale analysis of gene expression, molecular markers of cancer, noncoding RNAs. (fapesp.br)
  • This project aims to investigate the contribution of long non-coding RNAs (lncRNAs) to epigenetic reprogramming and control of gene expression in Pancreatic Ductal Adenocarcinoma (PDAC). (fapesp.br)
  • The type I target gene folded gastrulation is activated only in response to peak levels of the Dl gradient, so that expression is restricted to a subdomain of the presumptive mesoderm. (sdbonline.org)
  • The zerknullt target gene is repressed by high and low levels of the gradient, so that expression is restricted to the presumptive dorsal ectoderm. (sdbonline.org)
  • Heterogeneous population of RNA granules serve as motile units to translocate, store, translate, and degrade mRNAs in the dendrites contain cis -elements and trans -acting factors such as RNA-binding proteins and microRNAs to convey stimulus-, transcript-specific local translation. (frontiersin.org)
  • Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
  • RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
  • Phosphatidylinositol 4,5-bisphosphate (PI4,5P2, hereafter referred to as PIP2) either binds to intracellular proteins and directly modulates their subcellular localization and activity or acts as a precursor for the generation of other second messengers. (biomedcentral.com)
  • Consequently, motif quality is a major determinant of mRNA stability for Puf3 targets in vivo and can be used for the prediction of mRNA targets. (elifesciences.org)
  • The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and translation. (elifesciences.org)
  • Treatment with L-arginine has been shown before to regulate hyperglycemia and dyslipidemia7 and inhibit the polyol pathway8 in diabetic rats. (californiaehealth.org)
  • DmFKBP12 is first expressed at the syncytial blastoderm stage and undergoes increased expression during the cellular blastoderm and early gastrulation stages. (biomedcentral.com)
  • In higher eukaryotes, dynein/dynactin requires additional components such as Bicaudal D to form an active motor complex and for regulating its cellular localization. (biologists.com)
  • One of the best-characterized examples is oskar mRNA which localizes to the posterior region of Drosophila oocytes ( Besse and Ephrussi, 2008 ). (frontiersin.org)
  • In addition to temporal changes in expression, dynamic expression profiles during Drosophila development also revealed DmFKBP12 localization. (biomedcentral.com)
  • Throughout these changes in distribution, in situ DmFKBP12 mRNA monitoring detected equal distribution of DmFKBP12 mRNA, once again indicating that regulation of DmFKBP12 occurs at the translational level in Drosophila development. (biomedcentral.com)
  • As a critical regulator of the DmRyR-FKBP complex, DmFKBP12 expression in Drosophila fluctuates temporally and geographically with the formation of organ systems. (biomedcentral.com)
  • Both immunofluorescence and transmission electron microscopy analyses of cells overexpressing Clk/STY indicate that IGC components are completely redistributed to a diffuse nuclear localization, leaving no residual structure. (rupress.org)
  • Recent studies indicate that dys-regulation of microRNA expression plays important roles in tumorigenesis. (oncotarget.com)
  • This allows the cell to switch networks of related genes off together without affecting the mRNAs it still needs. (elifesciences.org)
  • Because the members of the PIP5KI and PIP4KII families exhibit specific and distinct subcellular localization patterns, it has been postulated that local increases in PIP2 concentration by a specific kinase member may underlie its diverse signaling functions [ 5 , 8 ]. (biomedcentral.com)
  • Some of the E(spl) complex also exhibit early expression in the presumptive mesectoderm. (sdbonline.org)
  • Here, we show that the genomic region surrounding human miR-31 at 9p21.3 is frequently deleted in many solid tumor cell lines, and reintroducing miR-31 into DAOY cells, a line of human medulloblastoma cells devoid of miR-31, strongly suppresses cell growth, causes cell cycle arrest at the G1/S boundary, and inhibits colony formation in vitro and xenograft tumorigenesis in nude mice. (oncotarget.com)
  • This means that when groups of mRNAs all carry the same motif, the cell can destroy them all together. (elifesciences.org)
  • Labelling different mRNAs with two colours of fluorescent dye showed how Puf3 helps the cell to choose which to destroy. (elifesciences.org)
  • The ability to destroy specific mRNA messages is critical for cell survival. (elifesciences.org)
  • Consequently, depletion of PLK1 has been shown to counteract the PIP4KIIγ depletion-induced instability of spindle pole-associated MT and cell resistance to arsenite. (biomedcentral.com)
  • Consistent with this finding, core components of EJC (eIF4AIII, Barentsz, Tsunagi, and Mago nashi) are critical for oskar mRNA localization ( Hachet and Ephrussi, 2001 , 2004 ). (frontiersin.org)
  • In addition to the cis -elements in its 3′UTR and trans -acting factor Staufen, posterior localization of oskar mRNA requires deposition of exon junction complex (EJC) in its first exon through splicing ( Hachet and Ephrussi, 2004 ). (frontiersin.org)
  • This enhancer contains a cluster of low-affinity Dl binding sites that restrict expression to the ventral-most regions of early embryos. (sdbonline.org)
  • A synthetic enhancer containing high-affinity Dl-binding sites and Twist binding sites exhibits expression in this region. (sdbonline.org)
  • First-strand complementary deoxyribonucleic acidity (cDNA) was synthesized in the poly-A formulated with messenger RNA (mRNA) as indicated below. (health-e-nc.org)
  • Here, we reconstitute accelerated and selective deadenylation of RNAs containing AU-rich elements (AREs) and Pumilio-response elements (PREs). (elifesciences.org)
  • Find support for a specific problem in the support section of our website. (mdpi.com)
  • WDFY2 showed labeling of two vesicle pools, a small pool negative for both APPL1 and EEA1, and one major pool, which is positive for EEA1. (nature.com)
  • What is puzzling is that the destabilising motifs that control different groups of mRNAs can be very similar, and scientists do not yet know how Ccr4-Not can tell the difference, or what triggers it to start breaking down groups of mRNAs. (elifesciences.org)
  • Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
  • The PE enhancer from the twist promoter region exhibits a similar pattern of expression. (sdbonline.org)
  • Our results show that treatment with L-arginine increased S-glutathiolation of AR in diabetic animals and that this was associated with a decrease in sorbitol accumulation, PKC activation, and ROS generation. (californiaehealth.org)
  • Puf3 could distinguish between mRNAs that differed in a single letter of genetic code. (elifesciences.org)
  • TABLE 1 Overview of sufferers and lesionsa for thirty minutes, top of the aqueous stage was used in a new pipe. (health-e-nc.org)