Mrna protein interactions. Medical search. Frequent questions

The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)
Similarly, a minimal ProQ/FinO domain protein, NMB1681, has been shown to bind a range of structured RNAs in Neisseria meningitidis 15 . (nature.com)
Insight into how these proteins recognize their cognate RNAs initiated with FinO. (nature.com)
A network of interacting RNAs and proteins is active in each of our cells. (embl.org)
A gigantic network of interacting RNAs and proteins is active in each of our cells, but we still know extremely little about the exact composition of this network. (embl.org)
We want to understand which proteins bind to RNAs , and how this differs between cell types, or in conditions when cells are stressed. (embl.org)
Protein interactions with all other RNA types - known as non-coding RNAs - could not be detected using the existing method. (embl.org)
Non-coding RNAs by far outnumber mRNA molecules, and they fulfill various regulatory purposes," explains Krijgsveld. (embl.org)
Here we present evidence that a third process impacts mRNA translation: interactions between noncoding RNAs and mRNAs. (edu.au)
We define the interactions between SARS-CoV-2 proteins and human RNAs. (cdc.gov)
NSP16 binds to the mRNA recognition domains of the U1 and U2 splicing RNAs and acts to suppress global mRNA splicing upon SARS-CoV-2 infection. (cdc.gov)
MicroRNAs (miRNAs) are small, noncoding RNAs that bind to the 3' UTR of target mRNAs, and silence gene expression by inducing degradation of target mRNAs or inhibition of protein translation [ 10 ]. (cdc.gov)

Orthologous to human LSM7 (LSM7 homolog, U6 small nuclear RNA and mRNA degradation associated). (nih.gov)
mRNA translation and degradation are mutually interdependent processes in the cell. (uni-muenchen.de)
It was shown for these pathways that mRNA degradation is initiated in a ribosome-dependent manner directly on the stalled intermediate. (uni-muenchen.de)
However, no structural insights exist on how translation and degradation are coupled for general mRNA turnover and quality control. (uni-muenchen.de)
The first part of this thesis focuses on the interactions of the Ski proteins with ribosomes in the exosome-dependent 3'-to-5' mRNA degradation pathway. (uni-muenchen.de)
Collectively, these results are the first structural insights into how translation and degradation of mRNAs are coupled on a molecular level. (uni-muenchen.de)
Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. (cancerindex.org)

We investigate the functional relevance of de novo missense variants, specifically whether they are likely to disrupt protein interactions, and nominate novel genes in risk for ASD through integrated genomic, transcriptomic, and proteomic analyses. (springer.com)
For genes encoding the disrupted interactions, we evaluate their expression patterns across developing brains and within specific cell types, using both bulk and inferred cell-type-specific brain transcriptomes. (springer.com)
Finally, we integrate protein interactions and cell-type-specific co-expression networks together with published association data to implicate novel genes in ASD risk in a cell-type-specific manner. (springer.com)
Genes encoding disrupted complementary interactors tend to be risk genes, and an interaction network built from these proteins is enriched for ASD proteins. (springer.com)
Consistent with other studies, genes identified by disrupted protein interactions are expressed early in development and in excitatory and inhibitory neuronal lineages. (springer.com)
Using the newly released and larger ASC dataset, we confirm these observations and take them in several new directions: (1) By defining a set of genes encoding these disrupted protein interactors in ASD subjects and another for their siblings, we evaluate their expression patterns in developing brain from fetal to early postnatal development and within general cell types of brain tissue. (springer.com)
A complex interaction between the cleavage and polyadenylation molecular complex and cis -elements determine the polyadenylation site, which may result in the choice of non-canonical sites, resulting in alternative polyadenylation events, involved in the regulation of more than 80% of the genes expressed in plants. (frontiersin.org)
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. (yeastgenome.org)
The present review focuses on circulating miRNAs in patients with TNBC, with an emphasis on their interaction with the immune response checkpoint genes PD‑1, PD‑L1 and CTLA4. (spandidos-publications.com)
One basic problem is how a decrease in tRNA levels, by downregulating Pol III efficiency, influences the expression pattern of protein-coding genes. (mdpi.com)
With such models, the interactions between genes, proteins or other multiomics data can be captured and studied, and provide valuable insight into their functional relationships. (biomedcentral.com)
The resulting hubs (i.e. genes or proteins with a high number of interactions) may again be used to identify central genes, functionally important proteins or pathway initiators, and thus potential drug targets [ 1 ]. (biomedcentral.com)
One setting where integrative network analysis has a lot of potential is when there are two types of data, e.g. measured mRNA and protein, associated with the same genes. (biomedcentral.com)
We identified >500 Drosophila genes that express mRNA isoforms with a long 3' UTR in proliferating spermatogonia but a short 3' UTR in differentiating spermatocytes due to APA. (stanford.edu)
The student will be responsible for improving the annotations, especially for the untranslated regions that lie up and downstream of protein coding genes. (lu.se)

Engineering chimeric, "humanized" yeast ribosomes for ES9S reveals that an evolutionary change in the sequence of ES9S endows species-specific binding of Hoxa9 mRNA to the ribosome. (nih.gov)
Sometimes, different types of RNA come together with specific proteins to form highly complex molecular machines - the best example is the ribosome where protein synthesis takes place. (embl.org)
A high resolution cryo-EM structure of a native ribosome-Ski complex reveals how the Ski complex interacts with the 40S subunit of the ribosome, facilitating the threading of mRNA into the Ski2 helicase. (uni-muenchen.de)
Ribosome profiling revealed that the deletion of EIF4A1 , but not EIF4A2 , rendered the translation of TOP mRNAs resistant to mTOR inactivation. (biorxiv.org)
NSP1 binds to 18S ribosomal RNA in the mRNA entry channel of the ribosome and leads to global inhibition of mRNA translation upon infection. (cdc.gov)

The RNA helicase eIF4A and the scaffold protein eukaryotic translation initiation factor 4G (eIF4G) and the capping protein eIF4E are part of the complex that loads the mRNAs onto the 40 S ribosomal subunit, together with eIF3. (biosyn.com)
In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
Eukaryotic translation initiation factor (eIF) 4A - a DEAD-box RNA-binding protein - plays an essential role in translation initiation. (biorxiv.org)
Alternative processing of nascent mRNAs is widespread in eukaryotic organisms and greatly impacts the output of gene expression. (stanford.edu)

ASH1 mRNA localization is dependent on four cis-acting localization elements as well as Myo4p, She2p, and She3p. (elsevierpure.com)
She2p is an RNA-binding protein that directly interacts with ASH1 cis-acting localization elements and associates with She3p. (elsevierpure.com)
The availability of multiple PAS in the same 3' regulatory region enables the inclusion or exclusion of 3' untranslated region (3'UTR) sequences, resulting in transcripts that may differ in particularities involving post-transcriptional processes such as stability, transport and translation ( Mayr, 2016 ), and even protein localization ( Berkovits and Mayr, 2015 ). (frontiersin.org)
PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. (springer.com)
APA can potentially affect mRNA translation efficiency, localization, stability, and mRNA seeded protein-protein interactions. (stanford.edu)
The interaction of ABHD11-AS1 with SART3 promotes USP15 (ubiquitin specific peptidase 15) nuclear localization. (cdc.gov)

Structural complexes between proteins and their substrates can be studied in the gas-phase to determine equilibrium dissociation constants. (biosyn.com)
Here, we review the structural elements required for designing mRNA vaccine constructs for effective in vitro synthetic transcription reactions. (mdpi.com)
Here, we investigate the structural basis for RNA recognition by ProQ/FinO proteins, through the crystal structure of the ProQ/FinO domain of the Legionella pneumophila DNA uptake regulator, RocC, bound to the transcriptional terminator of its primary partner, the sRNA RocR. (nature.com)
The role of adenovirus structural proteins in the regulation of adenovirus infection. (microbiologyresearch.org)
The protein HMGB1 behaves as a redox sensor and its structural changes, which are conditioned by the oxidative environment, are associated with different functions of the protein. (hindawi.com)
Our structural data supports a model where this interaction leads to the stabilization and orientation of the P-tRNA CCA-end to assist in peptide-bond formation, explaining eIF-5A's function as a general rescuing factor. (uni-muenchen.de)
Developments have been made in mRNA secondary structure prediction programs that use thermodynamics to construct possible structural models of mRNA sequences (Seetin and Mathews 2012). (columbia.edu)
The Arc gene, which contains remnants of a structural GAG retrotransposon sequence, produces a protein that self-assembles into capsid-like structures harboring Arc mRNA. (lu.se)
and capsid protein and minor structural protein mRNAs, complete cds. (cdc.gov)

A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. (nih.gov)
Our research studied the lncRNA and mRNA expression profiles of monocyte-derived DCs and demonstrated the functional networks that are involved in monocyte-derived DCs-mediated regulation in AR. (frontiersin.org)
Gene expression" refers to the process by which a DNA sequence (i.e. a gene) is used to make a specific RNA or protein, while "epigenetics" refers to the ways phenotypes are impacted by factors other than genetic sequence (e.g. gene repression by chromatin packing). (umassmed.edu)
The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
The candidate will be trained in mRNA Display, peptide synthesis, protein expression and purification, and biophysical analysis of target-ligand interactions. (gettinghired.com)
Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase. (stanford.edu)
In addition, spermatocyte-specific isoforms of Syncrip (Syp) are required for expression of CycB protein in mature spermatocytes and normal entry into the meiotic divisions. (stanford.edu)
Thus a set of spermatocyte-specific regulators choreograph the timing of expression of CycB protein during male meiotic prophase. (stanford.edu)
In addition, changes in APA mark a variety of pathological states, including many types of cancer, in which mRNAs are preferentially cleaved at more proximal PASs, causing expression of mRNA isoforms with short 3'UTRs. (stanford.edu)
Developmentally regulated alternate 3' end cleavage of nascent transcripts controls dynamic changes in protein expression in an adult stem cell lineage. (stanford.edu)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Model based on GA and DNN for prediction of mRNA-Smad7 expression regulated by miRNAs in breast cancer. (cancerindex.org)
In Paper I, we showed that miR-96 can upregulate the mRNA and protein expression of adhesion proteins E-Cadherin and EpCAM through direct interaction with target sites in the mRNA coding sequence. (lu.se)
This is not surprising given the strong evidence for interaction between myelin and axon gene expression in development and after experimental nerve lesions. (medscape.com)
Mounting evidence suggests that the genetic disorders/mutation and diseases change not only the protein expression patterns but also membranes themselves. (lu.se)

The ProQ/FinO family of RNA binding proteins mediate sRNA-directed gene regulation throughout gram-negative bacteria. (nature.com)
As CXXC1 is orthologous to Saccharomyces cerevisiae Spp1 that links DSB sites to the DSB machinery on the chromosome axis, we propose that these molecular interactions involved in the regulation of meiotic DSB formation are conserved in mouse meiosis. (springer.com)
Luciferases have become indispensable for the study of fundamental cellular processes including the regulation of transcription and translation, mRNA and protein stability, and nucleic acid/protein interactions. (neb.com)
With a minimalist model of metabolism, cell growth and transcriptional regulation in a microorganism, we explore how the interaction between environmental conditions and gene regulation set the growth rate of cells in the phase of exponential growth. (lu.se)

sRNAs usually work by pairing with target mRNAs, often with the assistance of protein partners called RNA chaperones. (nature.com)

DSB sites are preferentially located within chromatin loops, while several proteins that are required for DSB formation (Rec114, Mei4, and Mer2) localize on the chromosome axis (Blat et al. (springer.com)
RNA-immunoprecipitation sequencing (RIP-Seq) of the two eIF4A paralogs revealed that eIF4A1 preferentially binds to mRNAs containing terminal oligopyrimidine (TOP) motifs, whose translation is rapidly repressed upon mTOR inhibition. (biorxiv.org)

Our data show that the distinct protein interactions of these highly homologous translation factor paralogs shape protein synthesis during mTORC1 inhibition and provide a unique example of the repressive role of a universal translation activator. (biorxiv.org)
We provide a diverse portfolio of premium products to aid in your research, from tools and kits to isolate and purify total RNA to cells and enzymes that accelerate the synthesis of mRNA. (sigmaaldrich.com)
Find reagents for synthesis of mRNA. (sigmaaldrich.com)
Protein synthesis, folding, and tertiary and quaternary structure ultimately determine much of the body's structure and function. (msdmanuals.com)

Likewise, the ProQ/FinO domain-containing protein RocC of Legionella pneumophila interacts with only one trans -acting sRNA (RocR) to repress post-transcriptionally multiple mRNA targets 6 . (nature.com)
In S. cerevisiae , this interaction is at least in part provided by Spp1 that directly interacts with both methylated H3K4 near DSB sites and the axis-localized protein Mer2 (Acquaviva et al. (springer.com)
Nuclear localized USP15 interacts with pre-mRNA processing factor 19 (PRPF19) to increase CD44 RNA alternative splicing activating β-catenin and enhancing cancer stemness. (cdc.gov)

Furthermore, we show by ribosomal profiling analysis that this interaction is probably not limited to mRNA quality control, but might rather represent a general mRNA turnover intermediate. (uni-muenchen.de)

This machinery recognizes pre-mRNA sequences at several motifs - the 5′ and 3′ splice sites, the branch point, and polypyrimidine tracts - positioned at exon-intron boundaries [ 4 ]. (biomedcentral.com)
In principle, much of the variation in protein and mRNA levels should be predictable from genomic sequences. (edu.au)

High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis. (nature.com)
In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. (springer.com)
One pathway, which has been extensively studied in yeast, is mainly guided by chromatin structure and the other, analyzed in detail in mice, is driven by the sequence-specific DNA-binding PR domain-containing protein 9 (PRDM9). (springer.com)
To create a systems view of the signaling apparatus in budding yeast, we generated an epistatic miniarray profile (E-MAP) comprised of 100,000 pairwise, quantitative genetic interactions, including virtually all protein and small-molecule kinases and phosphatases as well as key cellular regulators. (thebiogrid.org)
The purpose of this study was to determine the mRNA levels in the yeast mutant rpc128-1007 and its overdose suppressors, RBS1 and PRT1. (mdpi.com)

The 5'- terminal ends of cellular mRNAs contain an m7GpppN cap, in which N can be any nucleotide. (biosyn.com)
Binding to dsRNA by these proteins may result in initiation of helicase activity, concomitant with a conformational change that leads to recruiting additional cellular factors, including the recently identified IPS-1/MAVS protein ( 5 , 6 ). (cdc.gov)
Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). (thermofisher.com)
Together with colleagues from EMBL, Krijgsveld has succeeded in developing a method called XRNAX to analyse the interactions of all RNA types with cellular proteins. (embl.org)
Reversible protein phosphorylation is a signaling mechanism involved in all cellular processes. (thebiogrid.org)
Mitochondrial porin , also known as the voltage-dependent anion channel (VDAC), is a multi-functional channel protein that shuttles metabolites between the mitochondria and the cytosol and implicated in cellular life and death decisions. (sdbonline.org)
It was shown that the ALG-TYR + P/5 min hydrogel exhibited favorable viscoelastic, morphological, mechanical, biocompatible, and cellular heat-shock protein amplification behaviors. (bvsalud.org)
Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)
Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)

Genome editing to site-specifically disrupt the Hoxa9-ES9S interaction demonstrates the functional importance for such selective mRNA-rRNA binding in translation control. (nih.gov)
Moreover, bioinformatic analyses were employed to identify the related biological functions of monocyte-derived DCs and construct the functional networks of lncRNAs and mRNAs that are differentially expressed (DE) in imDCs and mDCs. (frontiersin.org)
The model proposes the cap's interactions, with eIF4E, eIF4G, PABP, and the poly(A) tail and that the disruption of these interactions leads to functional effects. (biosyn.com)
Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. (thermofisher.com)
Synthetic mRNA is viewed as a viable and functional replacement for mature mRNA in both in vitro and in vivo research projects, including therapeutic development and delivery. (sigmaaldrich.com)
A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)

GenElute™ Direct mRNA Miniprep kit for smaller-scale purification. (sigmaaldrich.com)

Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)

We will apply this approach to a non-model sparrow study system to answer questions about how hybridization can disrupt evolved miRNA-mRNA interactions, leading to insights about the formation of new species. (lu.se)

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
Specifically, alternative cleavage and polyadenylation (APA) is a co-transcriptional molecular process that switches the polyadenylation site (PAS) at which a nascent mRNA is cleaved, resulting in mRNA isoforms with different 3'UTR length and content. (stanford.edu)
Plasmids purified from genomic DNA, proteins, ribosomes, and the bacterial cell wall are used in molecular biology research. (sigmaaldrich.com)

The 'closed-loop' model explains that the induction of 5'- to 3'-proximity of mRNAs requires the initiation factors eIF4E, eIF4G, and PABP. (biosyn.com)

This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
HMGB1 is an abundant protein, 10 6 molecules per cell [ 7 ], which has been postulated as a redox sensor [ 8 ]. (hindawi.com)
To carry out their vital tasks, all RNA molecules in our cells require proteins as binding partners. (embl.org)
RNA molecules perform vital tasks in every cell: messenger RNA (mRNA) carries genetic information from the DNA to be translated into proteins. (embl.org)
However, many other RNA molecules exist which are not translated into proteins. (embl.org)
For many of their functions, RNA molecules have to interact with proteins. (embl.org)
To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous. (lu.se)
To enable the tracking of Arc molecules from individual neurons in vivo, we devised an adeno-associated virus (AAV) mediated approach to tag the N-terminal of the mouse Arc protein with a fluorescent reporter using CRISPR/Cas9 homologous independent targeted integration (HITI). (lu.se)
CD117 by immunohistochemical methods sion molecules, involved in cell-cell and in order to clarify the role of the infiltrating cell matrix interactions and thought to take inflammatory cells in the pathomechanisms part in cell motility [ 2,3 ]. (who.int)

Predicted to be involved in mRNA splicing, via spliceosome. (nih.gov)
Stepwise sequential assembly of spliceosome components on these pre-mRNA motifs executes splicing reactions. (biomedcentral.com)
The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)
Mechanistically, it was found that ABHD11-AS1 directly binds SART3 (spliceosome associated factor 3, U4/U6 recycling protein). (cdc.gov)
It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. (thermofisher.com)

Dynamic m 6 A mRNA methylation directs translational control of heat shock response. (nature.com)
Previous work showed that the cell cycle regulator Cyclin B (CycB) is subject to translational repression in immature spermatocytes, mediated by the RNA-binding protein Rbp4 and its partner Fest. (stanford.edu)
Here we show that the spermatocyte-specific protein Lut is required for translational repression of cycB in an 8-hour window just before spermatocytes are fully mature. (stanford.edu)

Alternative polyadenylation (APA) generates transcript isoforms that differ in the position of the 3' cleavage site, resulting in the production of mRNA isoforms with different length 3' UTRs. (stanford.edu)

Moreover, eIF4A1 enhances the affinity between TOP mRNAs and LARP1 and thus ensures stronger translation repression upon mTORC1 inhibition. (biorxiv.org)

In eukaryotes, mRNA decay is intimately associated with translation. (igbmc.fr)
We are investigating how mRNA decay factors interact and/or interfere with the translation apparatus and how RNA modifications affect translation. (igbmc.fr)
The best characterized examples of the interplay between these two processes are the mRNA quality control pathways taking care of aberrant mRNAs that cause translation stalling in most cases. (uni-muenchen.de)

Dr. Pryciak studies how protein kinases regulate cell signaling and the cell cycle. (umassmed.edu)
4E-binding proteins (4E-BPs) block the interaction of eIF4E with eIF4G to negatively regulate the formation of the eIF4F complex. (biosyn.com)
miRNAs regulate proteins expressions by arresting or degrading the mRNAs. (cancerindex.org)
Here, we report that the SNARE-associated protein Sft2 acts downstream of the Arl1-Imh1 axis to regulate SNARE recycling upon ER stress. (bvsalud.org)

In accordance with its tumor suppressor role, the Retinoblastoma protein pRb can ensure pro-apoptotic functions. (sdbonline.org)

Recent updates are briefly described in the status of mRNA vaccines against SARS-CoV-2, influenza virus, and other viral pathogens. (mdpi.com)
The outcome of viral infections depends on a complex set of interactions between the viruses and their hosts. (cdc.gov)
These viral-host interactions shape the subsequent phases of the disease and influence the adaptive immune response. (cdc.gov)
The regulatory activities of this viral protein play a major role in the pathogenicity of influenza virus and appear partially responsible for the ability of influenza viruses to infect multiple animal species, which likely contributes to the generation of new pandemic viruses in humans. (cdc.gov)
Temperature sensitivity of processing of viral proteins. (microbiologyresearch.org)
Many protein-RNA interactions are suspected to be the underlying cause for diseases including cancer, amyotrophic lateral sclerosis, or viral infections like HIV. (embl.org)

The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. (cancerindex.org)

Extending earlier work, we show that de novo missense variants that disrupt protein interactions are enriched in individuals with ASD, often affecting hub proteins and disrupting hub interactions. (springer.com)

Trans -acting splicing factors, pre-mRNA secondary structure and chromatin organization are now known to directly affect alternative splicing decisions [ 6 ]. (biomedcentral.com)
Some variation can be accounted for by mRNA secondary structure and codon usage, both of which influence protein abundance. (edu.au)
We show that there has been strong selection for avoidance of off-target ncRNA:mRNA interactions across prokaryotes, and that, such interactions have a greater impact on protein abundance than either mRNA secondary structure or codon usage. (edu.au)
Although splicing is a complex process that is affected by multiple interactive variables, attempts have been made to use pre-mRNA secondary structure to explain how splicing decisions are made. (columbia.edu)
These findings advance our understanding of the influence of secondary structure on mRNA-protein interactions and encourage us to delve deeper into the complexities of splice site recognition. (columbia.edu)

article{3ef64d42-e741-442e-a1b5-bd0843c8bfaa, abstract = {{The activity-regulated cytoskeleton-associated (Arc) protein is essential for synaptic plasticity and memory formation. (lu.se)

The Smad7 protein is negative regulator of the TGF-β signaling pathway, which is upregulated in patients with breast cancer. (cancerindex.org)

High-throughput analysis of protein/peptide complexes by immunoprecipitation and automated LC-MS/MS". J Biomol Tech. 14 (2): 149-55. (wikipedia.org)

Changes in the redox state of cells affect proteins, lipids, and nucleic acids in different ways. (hindawi.com)
Due to the lack of detergent solubilization and reconstitution steps, the nSLBs created using this approach contain essentially all of the native lipids, as well as the membrane-associated proteins and carbohydrates from the donor membrane. (lu.se)

The unprecedently speedy development of mRNA vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was enabled with previous innovations in nucleoside modifications during in vitro transcription and lipid nanoparticle delivery materials of mRNA. (mdpi.com)
For in vitro delivery of mRNA for applications such as stem cell induction, protein replacement, cancer immunotherapies, or vaccine modalities, synthetic mRNA has become a critical tool in diverse biopharmaceutical approaches. (sigmaaldrich.com)

Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)

An Epistatic MiniArray Profile (E-MAP) analysis was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. (thebiogrid.org)
Genetic interaction can be defined as a deviation of the phenotypic quantitative effect of a double gene mutation from the effect predicted from single mutations using a simple (e.g., multiplicative or linear additive) statistical model. (researchgate.net)
Experimentally characterized genetic interaction networks in model organisms provide important insights into relationships between different biological functions. (researchgate.net)
We describe a computational methodology allowing to systematically and quantitatively characterize a Boolean mathematical model of a biological network in terms of genetic interactions between all loss of function and gain of function mutations with respect to all model phenotypes or outputs. (researchgate.net)
We demonstrate this methodology on three published models for each of which we derive the genetic interaction networks and analyze their properties. (researchgate.net)
In genetic interactions, synergy occurs when the contribution of two mutations to the phenotype of a double mutant exceeds the expectations from the additive effects of the individual mutations [74]. (researchgate.net)
In addition, it can be used to predict genetic interactions [3] by comparing the sensitivity value of a double gene mutation from the value predicted from single mutations, and reveal the network intervention [4] by applying the state-flip mutation subject to a single gene. (researchgate.net)

More recently, the application of RNA-seq technologies has enabled the elucidation of the biological partners and targets of several ProQ/FinO family proteins. (nature.com)
These results provide a mechanism by which Drosophila Bcl-2 family proteins can control apoptosis and shed light on a link between Rbf1 and mitochondrial dynamics, in vivo. (sdbonline.org)

What pathways are this gene/protein implicaed in? (cancerindex.org)

Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)
That messenger RNA (mRNA) abundances should broadly correlate with protein abundance is a critical assumption in transcriptomics. (edu.au)
Taking crosstalk interactions into account enables precise modulation of protein abundance. (edu.au)

These characteristic modifications that delimits full mRNAs become ultimately the targets of the mRNA decay machinery. (igbmc.fr)

In Saccharomyces cerevisiae, ASH1 mRNA is localized to the tip of daughter cells during anaphase of the cell cycle. (elsevierpure.com)
Messenger RNA (mRNA) regulates cell proliferation. (biosyn.com)
Scientists at the German Cancer Research Center (DKFZ) and EMBL have developed a method that enables researchers to analyse the composition of the entire protein-RNA network of the cell. (embl.org)
In fact, only 5% of RNA in a human cell is mRNA. (embl.org)
For example, neurons tend to express mRNAs with long 3'UTRs due to preferential processing at PASs more distal than other PASs used in other cell types. (stanford.edu)
Finally, NSP8 and NSP9 bind to the 7SL RNA in the Signal Recognition Particle and interfere with protein trafficking to the cell membrane upon infection. (cdc.gov)
This interaction enhanced cell migration of CRC cells and TAMs. (lu.se)
The enhanced levels of MMP-7 and -9 mRNA expressions and increased cell invasion into matrigel were observed in colon cancer cells upon stimulation with TAM-conditioned medium. (lu.se)

The eponymous FinO protein was discovered as a regulator of F plasmid conjugation nearly 50 years ago, and acts to bind a single partner sRNA called FinP to stabilize FinP and facilitate its interactions with its antisense partner, the mRNA encoding the major F plasmid transcription factor, TraJ 5 . (nature.com)
PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Krüppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. (springer.com)

With the success of COVID-19 vaccines, newly created mRNA vaccines against other infectious diseases are beginning to emerge. (mdpi.com)
Understand how mRNA vaccines induce immunity. (sigmaaldrich.com)

Gene and mRNA for precursor polypeptide VI from adenovirus type 2. (microbiologyresearch.org)

Moreover, Debcl and Drp1 can interact and Buffy inhibits this interaction. (sdbonline.org)

This review describes recent advances in the role of human HMGB proteins and other proteins interacting with them, in cancerous processes related to oxidative stress, with special reference to ovarian and prostate cancer. (hindawi.com)
Human recombinant protein fragment corresponding to amino acids 1-300 of human SLU7 produced in E.coli. (thermofisher.com)

A specific mRNA molecule is transcribed from each gene, which then can be translated into a specific protein. (biomedcentral.com)
A gene is a segment of DNA that provides the code to construct a protein or RNA molecule. (msdmanuals.com)

Figure 1: Chemical structures of mRNA Cap0, Cap1, and Cap2. (biosyn.com)

NanoFabTx™ reagent and microfluidic device kits provide a ready-to-use platform to screen multiple formulations, and ultimately synthesize mRNA or drug-encapsulated liposomes for preclinical assessments. (sigmaaldrich.com)

By proximity ligation assay (PLA), we demonstrated that the mCherry-Arc fusion protein retains the Arc function by interacting with the transmembrane protein stargazin in postsynaptic spines. (lu.se)
This new approach has been shown to preserve mobility and enzymatic activity of transmembrane proteins in the resulting nSLB. (lu.se)

The polyadenylation site (PAS), point from which the pre-mRNA is cleaved and polyadenylated, is defined by surrounding cis- elements ( Tian and Manley, 2016 ). (frontiersin.org)

Connecting all disrupted pairs of proteins, we construct an "ASD disrupted network. (springer.com)

The Massi lab uses their expertise in NMR Spectroscopy to understand the dynamics of protein-RNA interactions. (umassmed.edu)
While many studies have been focused on the membrane structure, the dynamics of such systems are crucial for the function of the membrane including membrane bound proteins. (lu.se)

Plasmid preparations can be contaminated with host DNA, RNA, and proteins. (bioprocessintl.com)

Lipid nanoparticles (LNPs) are critical vehicles for mRNA delivery - encapsulating and conveying mRNA safely to cells. (sigmaaldrich.com)

Anatomy4

Organisms2

Chemicals and Drugs16

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes23

Disciplines and Occupations3

Information Science9

Health Care1

RNAs12

Degradation7

Genes15

Ribosome5

Eukaryotic4

Localization6

Structural9

Expression15

Regulation4

Target mRNAs1

Preferentially2

Synthesis4

Interacts3

Ribosomal1

Sequences2

Yeast5

Cellular9

Functional6

Purification1

Stability1

Hybridization1

Molecular3

Initiation1

Molecules9

Spliceosome5

Translational3

Isoforms1

Inhibition1

Translation3

Regulate4

Tumor1

Viral6

Binds1

Disrupt1

Secondary5

Abstract1

Pathway1

Complexes1

Lipids2

Vitro2

Genomic1

Genetic7

Family proteins2

Pathways1

Abundance3

Decay1

Cell8

Transcription2

Vaccines2

Polypeptide1

Interact1

Human2

Molecule2

Structures1

Ultimately1

Transmembrane2

Polyadenylation site1

Construct1

Dynamics2

Plasmid1

Nanoparticles1