ProteinPolypeptideGeneGenesAntibodyEndoplasmic reticulumMoleculeSomaticSequencesSequenceMonoclonalFormalin-fixedTranscriptsLambdaAmyloidosisVaccineSpleenExpressionYieldsRestrictionSerumLociBone marrowMutantAmino acidsProteinsRegulationContrastAcidicCellsPlasma cellIncreaseTranscriptionLevelsVariableBetaHumanPatientsFullyAlphaMouseChemicalConsists
Protein9
- Deletion of the N-terminal part of the heavy chain disease protein leads to aggregation and signaling of the B cell receptor, presumably due to the loss of the anti-aggregating properties of the light chain. (wikipedia.org)
- Human immunoglobulin-related 14.1 protein mRNA, complete cds. (lu.se)
- Immunoglobulin lambda-like polypeptide 1 precursor (Immunoglobulin- related 14.1 protein) (Immunoglobulin omega polypeptide) (Lambda 5) (CD179b antigen). (lu.se)
- REAP-seq or RNA Expression and Protein Sequencing assay uses barcoded antibodies to label cells prior to scRNA-seq allowing simultaneous mRNA and Protein measurements. (einsteinmed.edu)
- 2012). In this study, we found that ACh could regulate the mRNA expression of osteocytic NPY via m- and n-AChRs without changing the protein levels (Fig. 6). (immunoglobulin-light-chain-variable-region-fragment.com)
- Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. (abcam.cn)
- Importin α-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion. (southernbiotech.com)
- Localized forms of amyloidosis appear to be caused by local production and deposition of an amyloidogenic protein (most often immunoglobulin light chains) within the affected organ rather than by deposition of circulating proteins. (msdmanuals.com)
- HDAC1 and vimentin were significantly increased in HCC cells, and HDAC1 overexpression enhanced vimentin mRNA and protein expression levels in an HDAC1 dose‑dependent manner. (spandidos-publications.com)
Polypeptide1
- The order of amino acids as they occur in a polypeptide chain. (lookformedical.com)
Gene3
- It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). (cancerindex.org)
- This gene encodes the CD8 alpha chain isoforms. (wikidoc.org)
- The hallmark of all BL tumours is the translocation between the MYC gene and one of the immunoglobulin (Ig) heavy or light chain loci. (bmj.com)
Genes2
- Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
- The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. (cancerindex.org)
Antibody1
- Structural analysis showed that this antibody targets a class 1/RBS-A epitope in the receptor binding domain via multiple hydrophobic and polar interactions with its heavy chain complementarity determining region 3 (CDR-H3), in addition to common motifs in CDR-H1/CDR-H2 of class 1/RBS-A antibodies. (cov19longhaulfoundation.org)
Endoplasmic reticulum1
- Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. (lookformedical.com)
Molecule1
- Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing. (academicinfluence.com)
Somatic2
- The defect in the immunoglobulins presumably arises during somatic hypermutation. (wikipedia.org)
- in particular the mechanisms of immunoglobulin variable (V) to diversity (D) and joining (J) element rearrangements and the mechanism of somatic hypermutation, reviewed in Steele and the basic similarities between the immunogenetics of Ig and T cell receptors (TCRs, see text Murphy et al. (sciencerepository.org)
Sequences1
- 5% of the captured cDNA is used in a targeted enrichment amplification to produce a library, which enables sequencing of full-length V(D)J segments including alpha and beta T-cell receptor or heavy and light chain immunoglobulin sequences from B cells. (einsteinmed.edu)
Sequence6
- Partial amino acid sequence of a kappa chain. (wikidata.org)
- Sequence at the 3' end of globin mRNA shows homology with immunoglobulin light chain mRNA. (wikidata.org)
- RNA-sequence analysis revealed that curcumin intake affected hepatic lipid metabolism via the peroxisome proliferator-activated receptor (PPAR) pathway, especially PPARα activation, resulting in increased Apoa2 mRNA expression. (elifesciences.org)
- and (ii) a light chain variable domain comprising a VLCDR1 having the amino acid sequence RASESVDNYGISFMN (SEQ ID NO: 6), a VLCDR2 having the amino acid sequence GASNQGS (SEQ ID NO: 7), and a VLCDR3 having the amino acid sequence QQSKEVP YT (SEQ ID NO: 8). (justia.com)
- Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)
- Subsequently, truncation and mutation of a vimentin promoter demonstrated that HDAC1‑induced vimentin expression was dependent on a nuclear factor κ‑light‑chain‑enhancer of activated B cells (NF‑κB) binding site in the vimentin promoter sequence. (spandidos-publications.com)
Monoclonal1
- AL is caused by overproduction of an amyloidogenic immunoglobulin light chain in patients with a monoclonal plasma cell or other B cell lymphoproliferative disorder. (msdmanuals.com)
Formalin-fixed2
- In such cases, formalin-fixed, paraffin-embedded (FFPE) tissues are required, but methods such as immunohistochemistry (IHC) may not be sensitive enough to detect light chain expression in B-cells. (biospace.com)
- Immunohistochemistry (IHC) or in situ hybridization (ISH) are commonly used to assess light chain restriction in formalin fixed, paraffin embedded (FFPE) tissues. (unm.edu)
Transcripts3
- The syntheiss of high yields of full-length reverse transcripts of globin mRNA. (wikidata.org)
- mRNA transcripts for osteopontin were determined using RT- -PCR with specific primers. (bvsalud.org)
- mRNA transcripts were detected in all culture conditions since the first day of culture. (bvsalud.org)
Lambda5
- MINNEAPOLIS, May 10, 2023 /PRNewswire/ -- Bio-Techne (NASDAQ: TECH), a global life sciences company providing innovative tools and bioactive reagents for the research and clinical diagnostic communities today announced the launch of two new RNAscope™ in situ Hybridization Probes Kappa and Lambda as analyte specific reagents (ASRs) for the detection of immunoglobulin kappa and lambda light chains mRNA in B-cells. (biospace.com)
- Immunoglobulin kappa and lambda chains are important biomarkers for the assessment of B-cell clonality. (biospace.com)
- Flow cytometry is the current gold standard for assessing kappa and lambda light chains, but it may not be effective for certain B-cell lymphomas that lack surface immunoglobulin expression. (biospace.com)
- RNAscope ISH Probes Kappa and Lambda are designed to detect immunoglobulin kappa and lambda light chains mRNA. (biospace.com)
- Ultrasensitive RNA in Situ Hybridization for Kappa and Lambda Light Ch" by Michael M. Franklin, Chelsey Deel et al. (unm.edu)
Amyloidosis1
- The United States is a major exception to this in that the immunoglobulin-related amyloid light chain type (AL) of amyloidosis is more frequent than AA as the cause of systemic amyloid deposition. (medscape.com)
Vaccine2
- The Omicron variants can escape recognition by COVID-19 vaccine-associated immunity to varying extents, thereby significantly reducing the neutralizing potency of serum antibodies from convalescent, fully mRNA-vaccinated individuals and individuals boosted with the new WT/BA.5 bivalent mRNA vaccine ( 9 , 10 ). (cov19longhaulfoundation.org)
- 5. Therapeutic Goods Administration (TGA) FOI Reply 2389-6, p.45 Nonclinical Evaluation Report: BNT162b2 [mRNA] COVID-19 Vaccine (COMIRNATYTM). (medicatrix.be)
Spleen1
- The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)
Expression2
- During the past decade, mRNA expression profiling has been successfully applied to the molecular characterization of breast cancers. (karger.com)
- Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. (cipsm.de)
Yields1
- 4. Kariko K., Muramatsu H., Welsh F.A., Ludwig J., Kato H., Akira S., Weissman D. Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. (medicatrix.be)
Restriction1
Serum2
- Diagnosis is made by the demonstration of an anomalous serum M component that reacts with anti-IgG but not anti-light chain reagents. (wikipedia.org)
- We purified free light chains (FLCs) from both urine and serum of healthy volunteers and individuals with acute PUUV illness, and verified the presence of antigen-specific FLCs. (sciencepop.org)
Bone marrow1
- This is characterized by a localization of the mutated heavy chains in extramedullary tissue, or solely in the bone marrow. (wikipedia.org)
Mutant1
- A mutant immunoglobulin light chain is formed by aberrant DNA- and RNA-splicing events. (wikidata.org)
Amino acids1
- Proteins are composed of long chains of amino acids that fold on themselves to form three-dimensional structures. (elifesciences.org)
Proteins3
- These heavy chain disease proteins have various deletions, mainly in their amino-terminal part, which causes the heavy chains to lose the ability to form disulfide bonds with the light chains. (wikipedia.org)
- Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
- The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits. (lookformedical.com)
Regulation1
- The present study showed a reciprocal regulation of RANKL and OPG mRNA caused by ACh via the cholinergic receptors, which resulted in a significant increase in the mRNA ratio of RANKL:OPG in osteocytes (Fig. 3B). (immunoglobulin-light-chain-variable-region-fragment.com)
Contrast1
- In contrast, mRNA levels are now shown not to change by both Northern and RNAse protection assays. (embl.de)
Acidic1
- Beta-crystallins exist as oligomers formed from acidic (BETA-CRYSTALLIN A CHAIN) and basic (BETA-CRYSTALLIN B CHAIN) subunits. (lookformedical.com)
Cells1
- Heavy chain disease is a form of paraproteinemia and plasma cell dyscrasia that involves the proliferation of cells producing immunoglobulin heavy chains. (wikipedia.org)
Plasma cell1
- However, except for cases with plasma cell differentiation, these techniques often fail to identify immunoglobulin light chains. (unm.edu)
Increase2
- [ 5 , 6 ] Under the influence of the inflammatory cytokine IL-6, hepatic transcription of the messenger ribonucleic acid (mRNA) for SAA may increase 1000-fold when exposed to an inflammatory stimulus. (medscape.com)
- This lack of increase contrasts with previous reports as does the mRNA length: this is 50% larger as confirmed by sequencing the 3' end. (embl.de)
Transcription1
- The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. (cancerindex.org)
Levels2
- NPY and reelin, two Peptone neurogenic markers, have been identified having higher mRNA levels in osteocytes than osteoblasts (Paic et al. (immunoglobulin-light-chain-variable-region-fragment.com)
- Cell density regulates prolyl 4-hydroxylase activity independent of mRNA levels. (embl.de)
Variable1
- Both alpha and beta chains share significant homology to immunoglobulin variable light chains. (wikidoc.org)
Beta2
- The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. (wikidoc.org)
- Beta crystallin a chain. (lookformedical.com)
Human1
- Alternatively spliced mRNA encodes a secreted form of human CD8 alpha. (wikidoc.org)
Patients2
- Immunoglobulin testing may be indicated for patients who develop repeated infections. (medscape.com)
- Immunoglobulin testing may be indicated for patients developing repeated infections. (medscape.com)
Fully1
- and -IgA in urine, nevertheless, the presence of intact immunoglobulins did not fully explain the LFRET signals. (sciencepop.org)
Alpha2
- There are four forms: alpha chain disease (Seligmann's disease) gamma chain disease (Franklin's disease) mu chain disease delta chain disease The most common type of heavy chain disease is the IgA type, known as αHCD. (wikipedia.org)
- A new mouse cardiac electrophysiology method was used to study mice harboring an alpha-myosin heavy chain Arg403Gln missense mutation (alpha-MHC403/+), which results in histological and hemodynamic abnormalities characteristic of familial hypertrophic cardiomyopathy (FHC) and sudden death of uncertain etiology during exercise. (jci.org)
Mouse1
- Mouse immunoglobulin chains. (wikidata.org)
Chemical1
- Chemical carcinogenesis was brought to light with the observations of Paracelsus on cancer onset and environmental exposure to chemicals undertaken over six centuries ago ( 1 - 3 ). (jcancer.org)
Consists1
- Furthermore, very small molecules, such as histamine (11.14 g/mol) which consists of an imidazole ring and a short carbon chain terminated with a primary amine, pose even more of a challenge due in part to their limited functionalities. (justia.com)