• Interestingly, it has been shown that platelets contain a broad spectrum of RNA molecules, including, in addition to mRNAs and miRNAs, also pre-mRNAs and a role of mRNA splicing in regulation of platelet protein synthesis has been proposed 18 , 19 . (nature.com)
  • Protective role of mRNA demethylase FTO on axon guidance molecules of nigro-striatal projection system in manganese-induced parkinsonism. (bvsalud.org)
  • The Overcoming COVID-19 network conducted a test-negative, case-control study at 20 pediatric hospitals in 17 states during July 1, 2021-January 17, 2022, to assess effectiveness of maternal completion of a 2-dose primary mRNA COVID-19 vaccination series during pregnancy against COVID-19 hospitalization in infants. (cdc.gov)
  • Using a test-negative, case-control study design, vaccine performance was assessed by comparing the odds of having completed a 2-dose primary mRNA COVID-19 vaccination series during pregnancy among mothers of case-infants and control-infants (those with negative SARS-CoV-2 test results) ( 6 ). (cdc.gov)
  • An mRNA vaccine termed BNT162b2 showed satisfactory safety and efficacy in the clinical trials but several issues including variability in the individual immune response or determination of target antibody titre that exerts sufficient immune protection needs to be assessed for planning a more efficient vaccination strategy. (researchsquare.com)
  • A new preprint study published in medRxiv suggests mRNA vaccines' efficacy against infection-particularly the Pfizer-BioNTech vaccine-has dropped significantly since the delta variant became widespread, although the vaccines' protection against hospitalization and death remain strong. (advisory.com)
  • Moreover, the researchers said vaccine efficacy could have been affected by other, unknown factors not accounted for in the study. (advisory.com)
  • This study includes 3 parts: Parts A, B, and C. The purpose of this study is to evaluate the immunogenicity and safety of mRNA-1010 seasonal influenza vaccine in adults. (clinicaltrials.gov)
  • Treated acute-onset COVID-19 mRNA vaccine-associated hypersensitivity events were relatively rare and mostly mild. (aaaai.org)
  • Reactions were more common with first COVID-19 mRNA vaccine doses than second doses. (aaaai.org)
  • The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
  • The polyadenylation site (PAS), point from which the pre-mRNA is cleaved and polyadenylated, is defined by surrounding cis- elements ( Tian and Manley, 2016 ). (frontiersin.org)
  • Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)
  • Polyadenylation is a two-step process, and this process is accomplished by multisubunit protein factors. (researchwithrutgers.com)
  • Here, we comprehensively compare the protein machinery responsible for polyadenylation of mRNAs across many evolutionary divergent species, and we have found these protein factors to be remarkably conserved in nature. (researchwithrutgers.com)
  • These data suggest that polyadenylation of mRNAs is an ancient process. (researchwithrutgers.com)
  • Hrp1 is a component of the cleavage and polyadenylation complex (CPF-CF) for pre-mRNA cleavage and polyadenylation in S. cerevisiae. (uni-goettingen.de)
  • We propose that Hrp1 might also be a guard protein that surveils pre-mRNA cleavage and polyadenylation and have carried out a series of experiments to support this idea. (uni-goettingen.de)
  • The variability of individual antibody responses, however, suggests the existence of factors other than age or male gender that affect the immune response to the vaccination. (researchsquare.com)
  • If we could have better understandings about those factors, we would be able to improve the vaccination strategy basing on the individual background. (researchsquare.com)
  • The teams from Henri-Mondor AP-HP hospital and Paris-Est Créteil University, Inserm, CNRS, University of Paris, within the Necker-Enfants Malades Institute, and the Institut Pasteur analyzed the capacity of the immune memory generated after mRNA vaccination to recognize and neutralize SARS-CoV-2 variants. (pasteur.fr)
  • Macy and co-workers from Kaiser Permanente Southern California recently published an article in The Journal of Allergy and Clinical Immunology: In Practice on the population-based incidence, severity, and risk factors associated with treated acute-onset COVID-19 mRNA vaccination-associated hypersensitivity reactions. (aaaai.org)
  • We have used in situ hybridization and immunocytochemistry to compare the nuclear localization of a specific nascent pre-mRNA and the essential non-snRNP splicing factor SC-35. (northwestern.edu)
  • Spector, David L. / Nascent pre-mRNA transcripts are associated with nuclear regions enriched in splicing factors . (northwestern.edu)
  • The capping apparatus is the first of the assemblies to act on the nascent pre-mRNA. (mskcc.org)
  • Are mRNA vaccines less effective against delta? (advisory.com)
  • There has been significant interest in acute-onset hypersensitivity reactions associated with the widespread use of COVID-19 mRNA vaccines, particularly since this was the first time this technology has been used in millions of people. (aaaai.org)
  • This report, like others, supports our growing confidence in the overall safety of COVID-19 mRNA vaccines. (aaaai.org)
  • I was excited to attend the launch event announcing the establishment of a hub for technology transfer of mRNA vaccines in South Africa. (who.int)
  • Most eukaryotic cellular mRNAs are modified at their 5 ́ends by the addition of a 7-methyl guanosine (m 7 G) residue in a 5 ́ → 5 ́ triphosphate linkage to the first encoded nucleotide of the transcript. (neb.com)
  • In RAW264.7 cells stimulated by lipopolysaccharide (LPS), n-3 DPA significantly down-regulated mRNA expression of pro-inflammatory factors such as IL-6, IL-1β, iNOS and COX-2. (unboundmedicine.com)
  • Furthermore, expression levels of IL-6 and IL-1β mRNAs were measured in the presence of the delta-6 desaturase inhibitor SC26196 in the culture medium to inhibit the conversion of n-3 DPA to DHA. (unboundmedicine.com)
  • There was no significant difference in the down-regulation in the mRNA expression of pro-inflammatory cytokines in RAW264.7 cells by n-3 DPA with or without presence of SC26196. (unboundmedicine.com)
  • In addition, we studied the mRNA expression of the six mIGFBPs during development and in various adult tissues. (eur.nl)
  • In liver, a peak of mIGFBP-1 mRNA expression was found around birth, suggesting a function for mIGFBP-1 in the newborn mouse. (eur.nl)
  • Expression of receptor proteins was examined by immunohistochemical analysis in paraffin-embedded prostatic tissue sections, and mRNA expression was determined by Northern blot and RT-PCR analysis. (nih.gov)
  • Moreover, there was a significant decrease in the expression of R-I and R-II mRNA, in all 20 primary prostatic tumors and 4 lymph nodes positive for metastases, indicating that the decreased protein expression was due to down-regulation of gene expression for the two receptors. (nih.gov)
  • there-after, GK mRNA expression, GK activity and insulin release were studied. (nih.gov)
  • Results demonstrated that biotin has a biphasic effect on GK mRNA expression, being stimulatory after short-term treatment and inhibitory after longterm treatment. (nih.gov)
  • The scaling factors will always be applied on the single-cell dataset first, altering its expression profiles accordingly, and then the pseudo-bulk samples are generated by summing up the count data from the sampled cells. (github.io)
  • We can also try out some custom scaling factors, for example increasing the expression levels for a single cell-type (T cells CD8) by 10-fold compared to the rest. (github.io)
  • Studies in animal and cellular models of human diseases showed that modified small nuclear RNAs (snRNAs) can promote changes in mRNA splicing and thus in gene expression. (unife.it)
  • We explored the snRNA-mediated rescue of coagulation factor VII (FVII) expression impaired by the IVS7+5 g/a mutation, which is associated to life-threatening bleeding in homozygous patients. (unife.it)
  • Expression of extended FVII minigenes in human hepatoma cells (Hep3B) and studies at the mRNA level (RT-PCR, fluorescent labeling and capillary electrophoresis) indicated that the IVS7+5g/a induces exon 7 skipping and activation of the first downstream cryptic 5'ss, thus generating frameshifts. (unife.it)
  • Depletion of Pop2, Not1, Not2, or Not3 subunits alleviates Pumilio RD-mediated repression of protein expression and mRNA decay, whereas depletion of other CNOT components had little or no effect. (umn.edu)
  • The authors examined the expression of EF1γ mRNA in 30 gastric carcinomas and their corresponding paired adjacent normal mucosae obtained during surgery while also examining 10 human digestive tract carcinoma cell lines using Northern blot hybridization. (elsevierpure.com)
  • Mechanistically, 3‑hydroxy‑3‑methylglutaryl‑CoA synthase 1 (HMGCS1) was identified as one of the direct downstream targets of VIM‑AS1, and VIM‑AS1 promoted HMGCS1 expression by enhancing HMGCS1 mRNA stability through a VIM‑AS1/insulin like growth factor 2 mRNA binding protein 2 (IGF2BP2)/HMGCS1 RNA‑protein complex. (spandidos-publications.com)
  • The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)
  • Our research studied the lncRNA and mRNA expression profiles of monocyte-derived DCs and demonstrated the functional networks that are involved in monocyte-derived DCs-mediated regulation in AR. (frontiersin.org)
  • PBMCs are divided into various functional subtypes with respect to the specific cytokine expression profiles, surface markers, and the transcription factors. (frontiersin.org)
  • The aim of this study was to assess the mRNA expression of MIF gene and its serum levels in the clinical manifestations of ACS and unrelated individuals age- and sex-matched with patients as the control group (CG). (hindawi.com)
  • ST-segment elevation myocardial infraction (STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI) showed 0.8 and 0.88, respectively, less expression of MIF mRNA with regard to CG. (hindawi.com)
  • Dysfunctional endothelium is a key factor in atherosclerosis that favors the increase of the expression of chemotactic and adhesion molecules (such as, intercellular adhesion molecule 1, ICAM1, and vascular cell adhesion molecule 1, VCAM1, as well as E-selectin and P-selectin) and enhanced recruitment and accumulation of monocytes [ 6 , 8 , 9 ]. (hindawi.com)
  • The B+/C+ genes show a significant change in mRNA expression for the 7 transcription factors for which deletion and ChIP data is available. (biomedcentral.com)
  • Because stromal cells are thought to influence glandular proliferation in the primate endometrium, we investigated the hormonal regulation and cellular localization of KGF mRNA expression in the rhesus monkey uterus. (elsevierpure.com)
  • Smooth muscle cells in the myometrium and the walls of the spiral arteries also expressed KGF mRNA, but the degree of this expression did not differ with hormonal state. (elsevierpure.com)
  • Moreover, sex differences in responses to pulmonary exposure to CNC were also detected at the level of global mRNA expression as well as in inflammatory cytokine/chemokine activity. (cdc.gov)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treat- ment. (lu.se)
  • This study aimed to investigate whether IGFBP7 levels and messenger ribonucleic acid (mRNA) expression are associated with the patient and tumor characteristics and prognosis in breast cancer. (lu.se)
  • IGFBP7 mRNA expression and its corresponding clinical data were obtained. (lu.se)
  • IGFBP7 mRNA expression and its corresponding clinical data were obtained from The Cancer Genome Atlas and analyzed for 809 patients. (lu.se)
  • Both low IGFBP7 protein levels and mRNA expression were associated with less aggressive tumor characteristics. (lu.se)
  • Gene regulation can occur at any point during gene expression, but most commonly occurs at the level of transcription (when the information in a gene's DNA is passed to mRNA). (medlineplus.gov)
  • Tissue repair was assessed by proliferating cell nuclear antigen (PCNA) immunoreactivity and by expression of keratinocyte growth factor (KGF) mRNA by real-time PCR. (cdc.gov)
  • KGF mRNA was constitutively expressed in both groups and did not increase significantly after Cl2 but expression was lower in TCR-delta-/- mice. (cdc.gov)
  • The results of in vivo and in vitro experiments showed that Mn exposure caused abnormal projection of dopaminergic neurons and decreased mRNA expression and protein levels of FTO. (bvsalud.org)
  • We have also compared the localization of c-fos transcripts with the speckled nuclear regions that are enriched in snRNPs and the non-snRNP splicing factor SC-35. (northwestern.edu)
  • This study demonstrates a direct link between specific nascent RNA transcripts and nuclear speckles that are enriched in pre-mRNA splicing factors. (northwestern.edu)
  • Currently, there is a growing body of evidence to suggest that RNA molecules around the size of full-length mRNA transcripts can be assayed in the supernatant of human urine and urinary extracellular mRNA could provide information about transcription in cells of urogenital tissues. (byrdlab.org)
  • Conversion of ~100% of 5′-triphosphorylated transcripts to capped mRNA is routinely achievable using enzyme-based capping. (neb.com)
  • They were able to identify all 391,123 unique Kaiser Permanente Southern California (KPSC) members who received COVID-19 mRNA vaccinations, first and/or second doses, between December 15, 2020, and March 11, 2021, at a KPSC facility. (aaaai.org)
  • Factor XIIIa is an enzyme of the blood coagulation system that crosslinks fibrin. (wikipedia.org)
  • We found that Hrp1 physically and genetically interacts with the mRNA export machinery Mex67-Mtr2 and directly contacts a component of the nuclear pore complex (NPC) named Mlp1, which is important for surveillance of mRNA export. (uni-goettingen.de)
  • Most strikingly, in comparison to the nuclear retention of faulty mRNAs in the exosome mutant rrp6Δ and the CPF-CF complex mutant cft2-1, these RNAs leak into the cytoplasm when functional Hrp1 is missing. (uni-goettingen.de)
  • Moreover, we discovered that Hrp1 binds more faulty mRNAs in cft2-1 but recruits less Mex67, which is consistent with its function in nuclear retention. (uni-goettingen.de)
  • Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. (rnagrade.com)
  • OBJECTIVE- Chronic activation of the nuclear factor-κB (NF-κB) in white adipose tissue leads to increased production of pro-inflammatory cytokines, which are involved in the development of insulin resistance. (diabetesjournals.org)
  • In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
  • abstract = "Pumilio is an RNA-binding protein that represses a network of mRNAs to control embryogenesis, stem cell fate, fertility and neurological functions in Drosophila. (umn.edu)
  • Changes in fibroblast growth factor 9 mRNA in granulosa and theca cells during ovarian follicular growth in dairy cattle. (ouhsc.edu)
  • Fibroblast growth factor receptor 1 contains three extracellular IMMUNOGLOBULIN C2-SET DOMAINS and is a tyrosine kinase that transmits signals through the MAP KINASE SIGNALING SYSTEM. (bvsalud.org)
  • See the reference protein sequence for complement factor H precursor (NP_569093.2). (nih.gov)
  • In fact, we were able to show that 3'-elongated mRNAs reached the cytoplasm in the hrp1-1 cft2-1 double mutant with cell fractionation experiments. (uni-goettingen.de)
  • Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. (rnagrade.com)
  • 0.05).During the formation and development of breast cancer, immune-related genes are always activated, and immunosuppressive factors CD274, IL8, MIF and VEGFA are upregulated. (preprints.org)
  • By contrast, the response of the B+/C- genes is insignificant for most transcription factors. (biomedcentral.com)
  • For what factors should we normalize urinary extracellular mRNA biomarkers? (byrdlab.org)
  • In this paper, we describe relevant first principles as well as research findings from our lab and other labs toward normalization of urinary extracellular mRNA. (byrdlab.org)
  • Budeme skúmať účinky exogénnej mRNA kodujúcej rastové faktory na neuronálnu excitabilitu použitím in vitro a in vivo elektrofyziológie, na neurogenézu použitím použitím značkovačov bunkového delenia a imunohistochemického farbenia a dendrito- a synaptogenéza bude sledovaná elektrónovou mikroskopiou. (sav.sk)
  • In vitro studies have shown that keratinocyte growth factor (KGF, also known as FGF-7) is secreted by fibroblasts and is mitogenic specifically for epithelial cells. (elsevierpure.com)
  • It specifically binds to a relatively conserved UA-rich domain called the efficiency-element (EE) upstream of the cleavage site to promote the accuracy and efficiency of pre-mRNA 3' processing. (uni-goettingen.de)
  • Factor XIII binds to the clot via their B units. (wikipedia.org)
  • We have shown that AUF1 induces EMT and stemness in breast epithelial cells via stabilization of the SNAIL1 and TWIST1 mRNAs, and their consequent upregulation. (nature.com)
  • Elongation factor 1 gamma (EF1γ) has been isolated from a pancreatic cancer cell line, while the overexpression of EF1γ has also been observed in colorectal carcinomas and adenomas. (elsevierpure.com)
  • In a clinicopathologic study, no relationship was observed between the overexpression of EF1γ mRNA and the histologic grading or depth of tumor invasion. (elsevierpure.com)
  • The overexpression of EF1γ mRNA was observed in both the gastric carcinoma tissue and in all the other cell lines examined. (elsevierpure.com)
  • Similar to the other guard proteins, overexpression of HRP1 is toxic to cells and retains mRNAs in the nucleus. (uni-goettingen.de)
  • Factor VII (FVII) is the plasma protease triggering coagulation, and its absence is lethal. (unife.it)
  • These findings suggest compensatory U1-snRNAs as therapeutic tools in coagulation factor deficiencies caused by mutations at 5'ss, a frequent cause of severe defects. (unife.it)
  • Catridecacog Coagulation D-dimer Factor XIII deficiency Muszbek L, Bereczky Z, Bagoly Z, Komáromi I, Katona É (July 2011). (wikipedia.org)
  • The product Assay kit for Guinea pig Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16(DHX16) (ELISA) is intended to be used for research purposes only. (rnagrade.com)
  • The product Assay kit for Guinea pig Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16(DHX16) (ELISA) should be kept between two and eight degrees Celsius to ensure the retention of the stability and reactivity of the reagents included in the kit. (rnagrade.com)
  • Time course of striatal DeltaFosB-like immunoreactivity and prodynorphin mRNA levels after discontinuation of chronic dopaminomimetic treatment. (lu.se)
  • In situ hybridization with nonradioactive digoxigenin- labeled cDNA probes revealed that the KGF mRNA signal, which was present only in stromal and smooth muscle cells, was substantially increased by P primarily in the stromal cells located in the basalis region. (elsevierpure.com)
  • Elongating RNA polymerase II is targeted by macromolecular assemblies that regulate mRNA synthesis and processing. (mskcc.org)
  • The evidence that platelets are capable of de novo protein synthesis in response to stimuli raised the issue of how megakaryocyte-derived mRNAs are regulated in these anucleate cell fragments. (nature.com)
  • Evidence that platelets are capable of de novo protein synthesis 17 also raised the issue of whether resident mRNAs are regulated in circulating platelets and, if so, why. (nature.com)
  • mRNA is a critical biomolecule involved in the manifestation of the genetic code into functional protein molecules. (byrdlab.org)
  • Transforming growth factor-beta (TGF-beta1) is a potent negative regulator of cell growth that transduces signals through interactions with type I and II receptors. (nih.gov)
  • Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei. (rupress.org)
  • We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. (rupress.org)
  • The snRNPs and an SR non-snRNP splicing factor are present in excess, mainly distributed throughout the interchromatin. (rupress.org)
  • Approximately 10% of the U2 snRNP and an SR non-snRNP splicing factor are associated with the chromosomes, highly enriched in active gene loci where they are bound to RNA. (rupress.org)
  • We demonstrate that the splicing factors are specifically recruited to a defined gene upon induction of transcription during physiological conditions. (rupress.org)
  • Concomitantly, the splicing factors leave gene loci in which transcription is turned off. (rupress.org)
  • We also demonstrated that upon general transcription inhibition, the splicing factors redistribute from active gene loci to the interchromatin. (rupress.org)
  • Our findings demonstrate the dynamic intranuclear organization of splicing factors and a tight linkage between transcription and the intranuclear organization of the splicing machinery. (rupress.org)
  • Splicing mutations in clotting factors, a relatively frequent cause of severe bleeding, represent ideal models to test this strategy, because tiny increases in functional full-length protein levels in patients significantly ameliorate hemorrhagic phenotypes. (unife.it)
  • These results indicate that RNA splicing events occur in platelets during activation and that maturation of specific pre-mRNAs is part of the activation cascade, contributing to a dynamic fine-tuning of the transcriptome. (nature.com)
  • In papillary renal cell carcinomas the TFE3 transcription factor becomes fused to the PSF and NonO pre-mRNA splicing factors and most commonly to a protein of unknown function designated PRCC. (uea.ac.uk)
  • In investigations of the normal function of PRCC we observed that PRCC expressed as a green fluorescent fusion protein colocalizes within the nucleus with Sm pre-mRNA splicing factors. (uea.ac.uk)
  • It was also found that endogenous PRCC is coimmunoprecipitated by antibodies that recognize a variety of pre-mRNA splicing factors including SC35, PRL1 and CDC5. (uea.ac.uk)
  • Several variants of the receptor exist due to multiple ALTERNATIVE SPLICING of its mRNA. (bvsalud.org)
  • We sought to identify the mechanism of Pumilio-mediated repression and find that it accelerates degradation of target mRNAs, mediated by three N-terminal Repression Domains (RDs), which are unique to Pumilio orthologs. (umn.edu)
  • To screen and validate immunosuppressive factors in luminal- and basal-like breast cancer cell lines and tissue samples associated with malignant phenotypes. (preprints.org)
  • A subunits of human factor XIII are made primarily by platelets and other cells of bone marrow origin. (wikipedia.org)
  • However, because individual immune-related factors can play several biological roles, the mechanistic relationship between immunosuppressive factors and breast cancer malignant phenotypes and the feasibility of their application as drug targets require further investigation. (preprints.org)
  • Moreover, bioinformatic analyses were employed to identify the related biological functions of monocyte-derived DCs and construct the functional networks of lncRNAs and mRNAs that are differentially expressed (DE) in imDCs and mDCs. (frontiersin.org)
  • We found that n-3 DPA treatment resulted in greater IL-6 mRNA down-regulation than that achieved with EPA treatment, and was similar to that of DHA treatment. (unboundmedicine.com)
  • Highest efficiency mRNA capping is achieved using the Faustovirus Capping Enzyme ( NEB #M2081 ). (neb.com)
  • Factor XIII levels are not measured routinely, but may be considered in patients with an unexplained bleeding tendency. (wikipedia.org)
  • This gene encodes one of the mitochondrial translation elongation factors. (nih.gov)
  • The insulin-like growth factor binding proteins (IGFBPs) comprise a family of six distinct proteins which modulate insulin-like growth factor action. (eur.nl)
  • The prognostic impact of insulin-like growth factor binding protein 7 (IGFBP7) in breast cancer is unclear. (lu.se)
  • Thus, we propose that Rna14 might be the trigger for Hrp1 mediated recruitment of Mex67 to mRNAs. (uni-goettingen.de)