Mrna aspartate ammonia ligase. Medical search. Frequent questions

Asparagine synthetase (or aspartate-ammonia ligase) is a chiefly cytoplasmic enzyme that generates asparagine from aspartate. (wikipedia.org)
Thus, after being released in, and channeled from, the glutaminase site, the ammonia molecule attacks the bound βAspAMP 1 to give asparagine and AMP via a tetrahedral intermediate. (wikipedia.org)
In mammals, asparagine synthetase expression has been found to be linked to cell growth, and its mRNA content is linked to changes in the cell cycle. (wikipedia.org)
Upregulation of asparagine synthetase mRNA was observed as well in these hamster cells. (wikipedia.org)
Other experiments demonstrated that quiescent rat thyroid cells entering S phase as a result of thyroid-stimulating hormone treatment was matched with a concurrent increase in asparagine synthetase mRNA content. (wikipedia.org)
There seem to be two major groups of asparagine synthetase: Majority of prokaryotic isolated enzymes (asnA) utilize ammonia as the sole nitrogen source. (wikipedia.org)
The lack of ammonia-dependent asparagine synthetase in eukaryotes is presumably because of the need to maintain cellular concentrations of ammonia at very low levels. (wikipedia.org)

After treatment with 1.92% arginine level diet to broilers or 300 µg/mL arginine culture medium to LMH cell line with FAdV-4 infection at the same time, we found that the mRNA levels of IL-6, IL-1ß, IFN-α and the protein levels of p-JAK2, p-STAT3 were down-regulated, compared with FAdV-4 infection group. (bvsalud.org)

JNK1 shRNA expressing INS1 cells demonstrated elevated apoptosis and cleaved caspase 9 and 3 in comparison to nonsense shRNA expressing control INS1 cells when subjected to palmitate and high blood sugar associated with elevated CHOP appearance, ROS development and mRNA appearance. (cgp60474.com)
JNK2 shRNA expressing INS1 cells didn't influence palmitate and high blood sugar induced apoptosis or ER tension markers, but increased appearance in comparison to non-sense shRNA expressing INS1 cells mRNA. (cgp60474.com)
Finally, JNK3 shRNA expressing INS1 cells didn't induce apoptosis in comparison to nonsense shRNA expressing INS1 cells when subjected to palmitate and high blood sugar but showed elevated caspase 9 and 3 cleavage connected with elevated and mRNA appearance. (cgp60474.com)

Regulates E3 ubiquitin-protein ligase activity of RNF19A (By similarity). (nih.gov)

The lack of ammonia-dependent asparagine synthetase in eukaryotes is presumably because of the need to maintain cellular concentrations of ammonia at very low levels. (wikipedia.org)
Individuals with HHH syndrome should be maintained on an age-appropriate protein-restricted diet, citrulline supplementation, and sodium phenylbutyrate to maintain plasma concentrations of ammonia, glutamine, arginine, and essential amino acids within normal range. (nih.gov)

Asparagine synthetase (ASNS) converts aspartate and glutamine to asparagine and glutamate in an ATP-dependent reaction. (nih.gov)
We then examined the mRNA levels of ASNS and ATF6 in 90 HCC patients, 77 chronic hepatitis B patients and 70 controls. (nih.gov)
The mRNA levels of ASNS and ATF6 were positively correlated with each other. (nih.gov)

The N-terminal active site catalyzes glutamine hydrolysis to yield glutamate and ammonia. (wikipedia.org)

The tunnel that links the two active sites allows for the passage of an ammonia molecule to act as a common intermediate to couple the two half-reactions carried out in the independent active sites of the enzyme. (wikipedia.org)
Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. (nih.gov)

Eukaryotic isolated and some prokaryotic isolated enzymes (asnB) utilize glutamine as the preferred nitrogen source, although these enzymes can also employ ammonia as an alternate substrate. (wikipedia.org)
Attention to subtle changes in mood, behavior, and eating and/or the onset of vomiting, which may suggest that plasma concentrations of glutamine and ammonia are increasing. (nih.gov)

Lack of reliable tools to rapidly evaluate PROTACs based on new ligases is hindering the progress. (pharmaceuticalintelligence.com)

PROTACs have been explored in multiple disease fields with focus on only few ligases like cereblon (CRBN), Von Hippel-Lindau (VHL), IAP and MDM2. (pharmaceuticalintelligence.com)