Mrna and protein protein clone cells. Medical search. Frequent questions

In molecular biology and genetics, a transcription factor is a protein that binds to specific DNA sequences, thereby controlling the flow of genetic information from DNA to mRNA. (absoluteastronomy.com)
Molecular chaperones are known to play a role in stabilizing protein structure and function during events of cellular stress. (bioone.org)
Therefore, it is important to explore candidate genes that could have significance to the clinical diagnosis, as well as the treatment of esophageal squamous cell carcinoma (ESCC) at the molecular level [ 4 ]. (hindawi.com)
The molecular mechanisms potentially responsible for cadmium carcinogenesis were investigated by differential gene expression analysis of Balb/c-3T3 cells morphologically transformed with cadmium chloride. (cdc.gov)
Immunoprecipitation experiments with anti-CR2 antibodies and SDS-PAGE analysis reveal a protein with an apparent molecular mass of 155 kDa which is higher than the one seen in B cells. (nih.gov)
Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)
In transfected COS7 cells the two long forms, HSPDE4B1 and HSPDE4B3, had molecular masses of approx. (ox.ac.uk)
1993). Previous studies have shown that neurexophilins are secreted proteins of small molecular weight with structural features that resemble those of neuropeptides, suggesting a possible signaling function mediated by alpha-neurexins (Petrenko et al. (uni-goettingen.de)

Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
Environmental injury has been associated with endoplasmic reticulum (ER) stress, a response characterized by activation of the unfolded protein response, proteasomal degradation of proteins, and induction of HSPA5, also known as GRP78 or BiP. (bioone.org)
mRNA degradation by nonsense-mediated decay (NMD) before translation, so no protein is produced (Fig. 1b). (horizondiscovery.com)
miRNAs exert their effects by suppressing the translation and often inducing the degradation of their target mRNAs. (nature.com)

Nucleophosmin has multiple functions: Histone chaperones Ribosome biogenesis and transport Genomic stability and DNA repair Endoribonuclease activity Centrosome duplication during cell cycle Regulation of ARF-p53 tumor suppressor pathway RNA helix destabilizing activity Inhibition of caspase-activated DNase Prevents apoptosis when located in nucleolus The NPM1 gene is up-regulated, mutated and chromosomally translocated in many tumor types. (wikipedia.org)
X-linked inhibitor of apoptosis (XIAP) is the most potent endogenous caspase inhibitor preventing cell death via caspase-9, -7 and -3 (initiator and executioner caspase pathways). (nih.gov)
SCARA5 promoted cell proliferation and migration but inhibited apoptosis in ESCC. (hindawi.com)
miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)
KLK6 is a secreted protein commonly overexpressed in CRC due to its effects on cell growth, apoptosis and metastasis. (arizona.edu)
Apoptosis is a genetically controlled cell death that was initially recognized for its role in development. (jneurosci.org)
Nearly half of neural cells die by apoptosis during brain development. (jneurosci.org)
The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
After antigen recognition and activation, CD8 + cytotoxic T cells may trigger keratinocyte apoptosis. (medscape.com)
Current data suggest that oral lichen planus (OLP) is a T-cell-mediated autoimmune disease in which autocytotoxic CD8 + T cells trigger the apoptosis of oral epithelial cells. (medscape.com)
Shan J, Ma JM, Wang R, Liu QL, Fan Y. Proliferation and Apoptosis of Peripheral Blood Mononuclear Cells in Patients with Oral Lichen Planus. (medscape.com)

The latest VOC, Omicron, carries 37 mutations in its spike protein, most of which lie within the receptor-binding domain (RBD), the target for neutralizing antibodies (nAbs). (news-medical.net)
The resulting antibodies reacted on immunoblots with the core protein of a detergent extracted heparan sulfate proteoglycan. (rupress.org)
When the anti-core protein antibodies were used to stain tissue sections immunoreactivity was present in peripheral nerve, newborn rat brain, heart, aorta, and other neonatal tissues. (rupress.org)
Our study shows that Jurkat human T cells have a molecule that reacts with both anti-CR2 antibodies and the third component of complement, C3. (nih.gov)
The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes. (rndsystems.com)
TIA1- and TIAL1-deficient GC B cells fail to undergo antigen-mediated positive selection, expansion and differentiation into B-cell clones producing high-affinity antibodies. (ox.ac.uk)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

On Northern blots the cDNA hybridized to a single 5.6-kb mRNA that was present in Schwann cells, neonatal rat brain, rat heart, and rat smooth muscle cells. (rupress.org)
Relative levels of heat shock protein (Hsp)70 were measured in developing and diapausing embryos of A. limnaeus using quantitative Western blots. (bioone.org)
Southern nalyses at medium and high stringency indicated that G. cingulata possesses ne gene for the secreted aspartic proteinase, and Northern blots indicated that gene expression was induced by exogenous protein and repressed by ammonium salts. (microbiologyresearch.org)

The parent (MCF-7) and tamoxifen-selected cell lines were characterized for protein expression of ER, progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) using immunohistochemistry (IHC). (umass.edu)
Recent findings in our laboratory and those of others, however, have shown that the EBV receptor is also present on T cells. (nih.gov)
Normally, upon the stimulations by growth factors (GFs), the receptor tyrosine kinases (RTKs) are activated, and then recruit PI3K protein complex on the membrane to generate phosphatidylinositol-3,4,5-triphosphate (PIP3) from phosphatidylinositol-4,5-biophosphate (PIP2) [ 2 ]. (biomedcentral.com)
The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. (rndsystems.com)
In contrast, based on ligand binding analysis and receptor level (as assessed using anti‑CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (3). (rndsystems.com)
Neurexophilins (Nphs) form a family of proteins that were discovered as components of the alpha-latrotoxin receptor, based on their strong binding to the synaptic membrane proteins alpha-neurexins (Petrenko et al. (uni-goettingen.de)
[ 5 ] Specifically, a novel oncogenic mutation ( FIP1L1-PDGFRA ), which results in a constitutively activated platelet-derived growth factor receptor-alpha ( PDGFRA ), has been invariably associated with a primary eosinophilic disorder and results in clonal lines of pathologic cells. (medscape.com)
Younes F, Quartey EL, Kiguwa S, Partridge M. Expression of TNF and the 55-kDa TNF receptor in epidermis, oral mucosa, lichen planus and squamous cell carcinoma. (medscape.com)

In contrast, the previously established ER-negative TMX2-28 cell line has decreased expression of both DOK7 and ELF5 and increased DNA methylation in the transcriptional start site region of these genes. (umass.edu)
Alternative splicing of mRNA generated from both the human and rat PDE4B genes produces long and short splice variants that have unique N-terminal splice regions. (ox.ac.uk)
Moreover, genes known to be important in the innate and adaptive immune responses of terrestrial mammals and in responses to stress and contaminant exposure have been targeted for cloning using PCR-based techniques. (vin.com)
A total of 62 dolphin genes of known stress or immune function have been cloned by targeted PCR (including immunoglobulins, T-cell receptors, cytokines, Toll-like receptors, stress proteins and toxin response proteins). (vin.com)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

These two cell types differed, however, in their NF-κB and AP-1 binding after lipopolysaccharide stimulation, possibly due to differences in the expression of the subunits that comprise these transcriptional complexes. (ersjournals.com)
This study characterizes the expression of hormone receptors, and the mRNA and DNA methylation levels of docking protein 7 (DOK7), and E74-like factor 5 (ELF5), in 21 novel tamoxifen-resistant cell lines and extends the findings to primary and recurrent human breast tumors. (umass.edu)
Expression of DOK7 and ELF5 is significantly up-regulated in half of the tamoxifen-selected cell lines as compared to the parental MCF-7. (umass.edu)
SCARA5 expression is significantly downregulated in various tumor tissues including liver cancer (LC), rectal cancer, and glioblastoma [ 15 , 16 ] and might affect cell proliferation and invasion abilities by the focal adhesion kinase (FAK) signaling pathway and epithelial-mesenchymal transition (EMT) [ 5 , 17 - 20 ]. (hindawi.com)
Transfection of mammalian cells with an expression vector containing either TIF3 or TEF-1d cDNA resulted in overexpression of the encoded protein. (cdc.gov)
qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
Mammalian neuropeptide Y receptors (NPYRs) show significant homology with sNPFR1 (ref. 7 ) and treatment of rat insulinoma cells with the mammalian sNPF orthologue NPY enhances their expression of insulin 5 . (nature.com)
MicroRNAs (miRNAs) are endogenous noncoding RNAs that act as post-transcriptional regulators of gene expression by binding to the 3′-untranslated region (UTR) of target mRNAs. (nature.com)
Expression levels of mouse Akt2 mRNA and Akt2 protein varied among tissues, with the highest levels in skeletal muscle. (nih.gov)
Akt2 expression was low in a multipotent fibroblast cell line, but it was upregulated when these cells were transformed with Myod and induced to differentiate into myocytes. (nih.gov)
The knockouts were successfully created and the effectiveness of the CRIPSR silencing was proven by real time PCR to measure mRNA expression, and ELISA to measure KLK6 protein secretion. (arizona.edu)
The heterozygous clones however, showed either normal expression of KLK6, or complete silencing of the gene, verified by the same methods mentioned. (arizona.edu)
Western blot and RT-PCR analyses were then used to detect Smad 7 protein and mRNA expression levels. (eurekamag.com)
The two clones showed an increased expression of MMP-2 protein and enhanced enzyme activity. (eurekamag.com)
It is possible that Smad 7 can alleviate the development of tissue fibrosis by upregulating the expression of MMP-2 and downregulating the expression of TIMP-2 in mesangial cells. (eurekamag.com)
CPEB3 overexpression had insignificant effects on gene expression in HT22 cells. (aging-us.com)
Injury-induced cytochrome c -specific cleavage of caspase-9 followed by activation of caspase-3 in mature brain correlated with marked increases in Apaf-1 and caspase-3 mRNA and protein expression. (jneurosci.org)
After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)
Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
For example, increased expression of LINC00958 attributes to cell metastasis in gastric cancer 7 . (researchsquare.com)
NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
Differential cell surface marker expression and divergent responses to differentiation conditions suggest that the NP subtypes may correspond to distinct maturation stages and represent distinct NP cell subpopulations. (biomedcentral.com)
In this study, we examined whether AdipoR mRNA expression in human myotubes correlates with in vivo measures of insulin sensitivity. (diabetesjournals.org)
AdipoR1 mRNA expression was positively correlated with in vivo insulin and C-peptide concentrations, first-phase insulin secretion, and plasma triglyceride and cholesterol concentrations before and after adjustment for sex, age, waist-to-hip ratio, and body fat. (diabetesjournals.org)
Expression of AdipoR2 mRNA clearly associated only with plasma triglyceride concentrations. (diabetesjournals.org)
In multivariate linear regression models, mRNA expression of AdipoR1, but not AdipoR2, was a determinant of first-phase insulin secretion independent of insulin sensitivity and body fat. (diabetesjournals.org)
Due to the conflicting reports published, use of monoclonal MAB4227 may result in an underestimation of CXCR7 expression on certain cell types. (rndsystems.com)
Reports of mRNA levels and/or protein expression (as assessed using anti‑CXCR7, clone 9C4) (1, 2) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. (rndsystems.com)
PM 2.5 increased the mRNA and protein expression of M1 markers including interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α) and inducible nitric oxide synthase (iNOS) in RAW264.7 cells. (medsci.org)
PM 2.5 decreased the protein level of SIRT1 but not the mRNA expression in vitro and in vivo experiments. (medsci.org)
Overexpression of miR-146a-3p downregulated the expression of SIRT1 protein in untreated RAW264.7 cells. (medsci.org)
Mechanistically, TIA1 and TIAL1 control the transcriptional identity of dark- and light-zone GC B cells and enable timely expression of the prosurvival molecule MCL1. (ox.ac.uk)
Two cDNA libraries from known health status dolphin PBL have been generated including an IL-2 and an LPS-stimulated cDNA library, respectively biased towards T and B-cell gene expression. (vin.com)
1998). In parallel to the in vivo approach, a bacterial protein expression system was established, leading to the production of recombinant Nph1 and Nph3 for use in future in vitro experiments.Analysis of Nph3 expression in Nph3-KI mice was performed by assaying brain sections for beta-galactosidase activity, based on the co-expression of beta-galactosidase from Nph3-expressing cells. (uni-goettingen.de)
This method revealed the Nph3 expression pattern with high accuracy and sensitivity, suggesting a novel approach for the detailed in vivo identification of cells that express a particular protein. (uni-goettingen.de)
A highly localized Nph3 expression pattern was observed with the most characteristic area of expression being the deep layers of the neocortex, where a band of expressing cells persisted throughout ontogeny and adulthood. (uni-goettingen.de)
In addition, expression of Nph3 was observed in a number of transient cell populations in the marginal zone of the developing neocortex, the dentate gyrus of the hippocampal formation and the granule cell layer of lobules 9 and 10 of the cerebellum, where low expression levels were also observed in adulthood. (uni-goettingen.de)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

The growth and metastasis abilities of cells are evaluated in vitro and in vivo. (biomedcentral.com)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)

It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
In addition, cluster of differentiation 24 (CD24)-negative NP responder clones formed spheroid structures in various culture systems, suggesting the preservation of a more immature phenotype compared to CD24-positive nonresponder clones. (biomedcentral.com)

Two classes of intracellular proteins act as inhibitors of G protein activation: GTPase activating proteins (GAPs), which enhance GTP hydrolysis and guanine dissociation inhibitors (GDIs), which inhibit GDP dissociation. (embl-heidelberg.de)
The cells were then fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, and stained with 0.06 µg of Rat IgG2a kappa Isotype Control, APC (Product # 17-4321-81) (left) or 0.06 µg of CXCL13 Monoclonal Antibody, APC (right). (thermofisher.com)
Two differentially regulated mRNAs with different 5' ends encode secreted and intracellular forms of yeast invertase. (microbiologyresearch.org)

Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
HN - 2008 BX - Von Ebner's Glands MH - Cumulus Cells UI - D054885 MN - A05.360.319.114.630.535.200.500 MN - A06.407.312.497.535.300.500 MN - A11.436.300.500 MS - The granulosa cells of the cumulus oophorus which surround the OVUM in the GRAAFIAN FOLLICLE. (bvsalud.org)
HN - 2008 BX - Granulosa Cells, Cumulus MH - Coronary Sinus UI - D054326 MN - A07.231.908.194.500 MS - A short vein that collects about two thirds of the venous blood from the MYOCARDIUM and drains into the RIGHT ATRIUM. (bvsalud.org)

In a subsequent angiotensin-converting enzyme 2 (ACE2)-spike surrogate neutralization assay, P5C3, and P2G3 exhibited broad and highly potent inhibition of binding (of VOC spike proteins) to ACE2. (news-medical.net)
Author Manuscript and RNA repair protein AlkB-derived enzyme mixture was first used to remove methylations at the Watson-Crick face. (cdc.gov)

We show via an in vitro binding assay that miR-9a binds to sNPFR1 mRNA in insect cells and to the mammalian orthologue NPY2R in rat insulinoma cells. (nature.com)

In the mammalian brain, alternative pre-mRNA splicing is a fundamental mechanism that modifies neuronal function dynamically where secretion of different splice variants regulates neurogenesis, development, pathfinding, maintenance, migration, and synaptogenesis. (aging-us.com)

DNA and Cell Biology. (wikipedia.org)
X. laevis is the only amphibian where true lymphoid tumors have been discovered and cell lines have been obtained opening new avenues for tumor biology and tumor immunity. (rochester.edu)

A ribonuclease protection assay was used to quantitate levels of the core protein mRNA. (rupress.org)

Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
The human gastric epithelial permanent cell line (GES-1), human gastric cancer cell lines (AGS, BGC-823, MKN45, and SGC-7901), and HEK293 cells were provided by the Biomedical Experiment Center of Xi'an Jiaotong University. (hindawi.com)
EBV infects cells via the CR2 that was previously thought to be expressed only on the surface of B cells and certain epithelial cells. (nih.gov)
We detected severe acute respiratory syndrome coronavirus 2 antigen in alveolar epithelial cells and macrophages. (cdc.gov)
Coronavirus disease is essentially a lower respiratory tract disease characterized by direct viral injury of alveolar epithelial cells. (cdc.gov)

[ 4 ] In 3 patients with T-cell lymphomas, eosinophilia has been correlated with increased production of these cytokines by the lymphomas. (medscape.com)
Activated CD8 + T cells (and possibly keratinocytes) may release cytokines that attract additional lymphocytes into the developing lesion. (medscape.com)
Les numérations plaquettaire et leucocytaire ainsi que la concentration en cytokines (IL-2R, IL-8 et TNF-) ont été me- surées. (who.int)

Mutation at Y317 of p110α reduces the proliferation, migration, and invasion of cancer cells through Src-MLC2 pathway, while mutation at Y508 of p110α impairs AKT signaling. (biomedcentral.com)
Long noncoding RNAs (lncRNAs) regulate a number of physiological and pathological processes, including gene transcription and translation, chromatin modification, cell cycle progression, cell proliferation, and oncogenic and tumor-suppressive signals in cancer 6 . (researchsquare.com)

We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)

A cDNA clone coding for a membrane proteoglycan core protein was isolated from a neonatal rat Schwann cell cDNA library by screening with an oligonucleotide based on a conserved sequence in cDNAs coding for previously described proteoglycan core proteins. (rupress.org)
When making a knockout cell line using CRISPR, the first step is to isolate edited clones and sequence them to check for the presence of a frameshift mutation. (horizondiscovery.com)
The gcsap open reading frame encodes a protein with a 78 residue prepro-sequence typical of other fungal secreted aspartic proteinases. (microbiologyresearch.org)
Single-cell sequencing has made considerable progress relying on the notion that the sequence is the tag. (nature.com)
One of the clones possesses an identical nucleotide sequence to the one corresponding to B cell CR2, whereas the other represents a differentially spliced transcript which lacks the exon 8b of B cell CR2. (nih.gov)
Sequence analysis revealed that, like its human homolog, Akt2 encodes a protein-serine/threonine kinase containing a pleckstrin homology domain at its amino terminus. (nih.gov)
Sequence-specific RNA-Binding Protein CPEB3 has distinctive isoform-distinct biochemical interactions and neuronal development assembly roles. (aging-us.com)
Random Expressed Sequence Tag (EST) clones have been isolated and sequenced from dolphin Peripheral Blood Leukocyte (PBL) cDNA libraries. (vin.com)
Homo sapiens chromosome 17, clone hRPK.192_H_23, complete sequence. (lu.se)

The dense subepithelial mononuclear infiltrate in oral lichen planus is composed of T cells and macrophages, and there are increased numbers of intraepithelial T cells. (medscape.com)
Therefore, early in the formation of oral lichen planus lesions, CD8 + T cells may recognize an antigen associated with the major histocompatibility complex (MHC) class I on keratinocytes. (medscape.com)
[ 8 ] T cells in oral lichen planus contain mRNA for TNF and secrete TNF in vitro. (medscape.com)
The role of autoimmunity in the pathogenesis is supported by many autoimmune features of oral lichen planus, including its chronicity, onset in adults, predilection for females, association with other autoimmune diseases, occasional tissue-type associations, depressed immune-suppressor activity in patients with oral lichen planus, and the presence of autocytotoxic T-cell clones in lichen planus lesions. (medscape.com)
Sugerman PB, Satterwhite K, Bigby M. Autocytotoxic T-cell clones in lichen planus. (medscape.com)

cSH2 domain S690 phosphorylation decreases p85 binding affinity to tyrosine-phosphorylated proteins resulting in less PI3K membrane localization [ 15 ]. (biomedcentral.com)
A Nph3-KI targeting vector was designed in a way to result in the generation of a bicistron ic mRNA, encoding for a fusion Nph3-Flag protein, as well as for beta-galactosidase fused to a nuclear localization signal. (uni-goettingen.de)

Two cDNA clones were identified upon screening of a Jurkat cell cDNA library with the B cell CR2 cDNA. (nih.gov)

In heterotrimeric G-protein signalling, cell surface receptors (GPCRs) are coupled to membrane-associated heterotrimers comprising a GTP-hydrolysing subunit G-alpha and a G-beta/G-gamma dimer. (embl-heidelberg.de)

It is a serine protease ( EC 3.4.21.68 ) found on endothelial cells , the cells that line the blood vessels . (wikidoc.org)
The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)

For example, mRNA where the targeted exon is skipped may be present alongside mRNA including the targeted exon. (horizondiscovery.com)
As a first step, the Nph3 gene was cloned and its exon-intron structure was mapped. (uni-goettingen.de)

Abstract Starting from mature vegetable compost, four bac- the utilization of these bacteria as cell factories for upgrading terial strains were selected using a lignin-rich medium. (lu.se)

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
The western blot in Figure 2 uses an antibody recognizing an epitope in the N-terminus of the wildtype protein. (horizondiscovery.com)
Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)

The prokaryotes are a group of organisms that lack a cell nucleus , or any other membrane-bound organelles. (absoluteastronomy.com)
The second messenger PIP3 recruits and activates 3-phosphoinositide dependent protein kinase-1 (PDK1) and AKT serine/threonine kinase on the membrane [ 2 ]. (biomedcentral.com)
Tyrosine phosphorylation of RTKs, or adaptor proteins such as IRS-1 facilitate their binding with p85 regulatory subunits of PI3K, and then bring PI3K complex on membrane [ 2 ]. (biomedcentral.com)

Single-cell omics provide insight into cellular heterogeneity and function. (nature.com)
Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
tRNAs are essential for cells and their synthesis is under stringent cellular control. (cdc.gov)

X. laevis LG15 cloned adult injected sc with 10,000 15/0 tumor cells. (rochester.edu)
The 15/0 tumor line derived from the LG-15 clone (MHC a/c haplotype), grows well in LG-15 (Fig. 3) and other LG sharing the same MHC (LG6 and 7). (rochester.edu)
They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)

Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)

Nucleophosmin (NPM), also known as nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. (wikipedia.org)

Other types of cell lines are also available in X. laevis including: fibroblasts lines of the different strains and LG clones, and a kidney cells line (A6). (rochester.edu)

XIAP mRNA levels were established by RT-PCR, the four X (XIAP knockdown) clonal cell lines show 82-93% reduction in XIAP mRNA when compared to the four L (luciferase control) cell lines. (nih.gov)
In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)

This protease activity has recently been ascribed to serine metalloprotease(s), including pregnancy-associated plasma protein-A (PAPP-A), which was first detected in human follicular fluid nearly 20 yr ago. (bioone.org)

The KEGG and GO analyses indicated 'Human T − cell leukemia virus 1 infection' and 'Hippo signaling pathway' were the main affected biological processes. (researchsquare.com)
To further identify the potential signaling pathways after the knockdown of LINC00958 in Ishikawa cells, we performed the KEGG and GO analyses (Figure 2). (researchsquare.com)

Eosinophils in hypereosinophilic syndrome infiltrate multiple organs where they inflict tissue damage through the release of granule proteins, including eosinophil peroxidase, major basic protein, eosinophil-derived neurotoxin, and eosinophil cationic protein. (medscape.com)

Endogenous substances are those that originate from within an organism, tissue, or cell. (absoluteastronomy.com)
A provirus is a virus genome that is integrated into the DNA of a host cell.This state can be a stage of virus replication, or a state that persists over longer periods of time as either inactive viral infections or an endogenous retrovirus. (absoluteastronomy.com)

Therefore, we co-cultured AML cells with macrophages. (frontiersin.org)
Macrophages are the most abundant white blood cells in the immune microenvironment and have a high degree of heterogeneity. (frontiersin.org)

Importantly, inhibition of miR-146a-3p upregulated SIRT1 protein and suppressed M1 polarization in PM 2.5 treated RAW264.7 cells. (medsci.org)

Forkhead box protein N1 (Transcription factor winged-helix nude). (lu.se)

The basic structural material of hair is the protein keratin, a long, coiled chain of amino acids. (mcgill.ca)

If the mRNA is destroyed by NMD, or if a splice variant without the N-terminus is generated, bands will not be observed on the blot. (horizondiscovery.com)

The mRNA levels of ER, DOK7, and ELF5 were assessed using quantitative RT-PCR. (umass.edu)
High levels were found in neonatal rat brain, heart, and Schwann cells. (rupress.org)
Challenge of rGMCs with 1 or 10 μM HgCl 2 or Pb 2 acetate increased HSPA5 mRNA and protein levels. (bioone.org)

Both technologies have been primarily focused on measurement of protein epitopes, but have been used to measure nucleic acids such as targeted mRNA 11 , 12 . (nature.com)

Hypereosinophilic syndrome etiology can involve (1) primitive involvement of myeloid cells, essentially due to the occurrence of an interstitial chromosomal deletion on band 4q12 leading to the creation of the FIP1L1-PDGFRA fusion gene (F/P + variant), or (2) increased interleukin (IL)-5 production by a clonally expanded T-cell population (lymphocytic variant), most frequently characterized by a CD3 - CD4 + phenotype. (medscape.com)

Microdroplets can barcode more cells but worries persist about the differential chemistry and resulting biases accomplished in each droplet. (nature.com)

RNA-seq results showed that transcription factors and macrophage inflammatory proteins were significantly downregulated in the DNMT3A mutant clones. (frontiersin.org)

Quantum barcoding (QBC) avoids isolation of single cells by building cell-specific oligo barcodes dynamically within each cell. (nature.com)
TRI reagent ® is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. (sigmaaldrich.com)
A single reagent that can be used for RNA, DNA and protein isolation that is free of any contamination. (sigmaaldrich.com)

Homogenization or lysis of the tissue sample in TRI reagent ® dissolves RNA, DNA and protein. (sigmaaldrich.com)
TRI Reagent ® performs well with large or small amounts of tissue or cells and works with many samples including human, plant, yeast, bacterial and viral samples. (sigmaaldrich.com)
The whole process of RNA extraction starting with fresh tissue or cells can be completed in less than one hour. (sigmaaldrich.com)
To investigate matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) expressions in the cultured rat mesangial cells (MsC) transfected with Smad 7 vector and to elucidate the mechanism of Smad 7 in blocking tissue fibrosis. (eurekamag.com)
Tissue plasminogen activator (abbreviated tPA or PLAT ) is a protein involved in the breakdown of blood clots . (wikidoc.org)
The initial stages of DDD correlate with reduced cellularity, aberrant cell function, loss of proteoglycans and concomitant tissue dehydration [ 6 ]. (biomedcentral.com)

Human HCT116 colon cancer cell lines were transfected with either XIAP (X) or luciferase (L) shRNA. (nih.gov)
Method: A 1.1 kb mTRAF6 promoter region was cloned into pCR-BluntII-TOPO vector, after which a promoter-less pGL4-mTRAF6-luciferase construct was engineered. (umich.edu)

Using short hairpin RNA (shRNA) against XIAP, stably expressed in a parent HCT116 human colon cancer cell line, a series of clones have been developed. (nih.gov)
Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)

Six B-cell clones were prioritized for mAb synthesis by expressing light and heavy chains in ExpiCHO cells. (news-medical.net)
Regulation and synthesis of cell-walldegrading enzymes of plant pathogens. (microbiologyresearch.org)
With minimal instrumentation (four 96-well plates and a multichannel pipette), cell-specific codes are added to each tagged molecule within cells through sequential rounds of classical split-pool synthesis. (nature.com)

Using western blotting for verification can be a helpful tool, but correct interpretation of the results is crucial to avoid misidentification of cell lines with functional proteins as knockouts (and vice versa). (horizondiscovery.com)
Here we created KLK6 knockouts and heterozygous clones from the SW480 cell line and measured cell growth and viability over multiple days. (arizona.edu)

In addition to the fibrillar amyloid protein, the deposits also contain serum amyloid P component and glycosaminoglycans. (msdmanuals.com)

Microorganisms capable of catabolizing lignin-derived ar- carbohydrate and protein components of the organic matter omatics are essentially fungi and bacteria (Bugg et al. (lu.se)

Except for the B3B3 line that has undergone unproductive gene rearrangement, all other lines express IgH and L mRNAs but do not synthesize any Ig protein (Robert et al. (rochester.edu)
In this blog post we will look at the possible fates of a protein after gene knockout using CRISPR and explore how best to validate a knockout cell line. (horizondiscovery.com)
Some of these fates can co-occur in the same cell line. (horizondiscovery.com)
DNA, mRNA, and protein in a wildtype cell line (a) and possible outcomes after a frameshift mutation (b-f). (horizondiscovery.com)
Why are bands visible in a western blot using a knockout cell line? (horizondiscovery.com)
The targeting vector was inserted into ES cells in culture and a Nph3-KI mouse line was generated (blastocyst injections and transplantations were performed in collaboration). (uni-goettingen.de)

GC B cells rely on post-transcriptional RNA mechanisms to translate activation-associated transcriptional programs into functional changes in the cell proteome. (ox.ac.uk)

Thrombospondin type 1 domain-containing 7A ( THSD7A ) was reported to play a procancer role in esophageal squamous cell carcinoma (ESCC). (hindawi.com)

Anatomy29

Organisms20

Diseases2

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes86

Disciplines and Occupations1

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Molecular9

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Apoptosis11

Antibodies7

Blots3

Receptor8

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Expression40

Vitro and in vivo2

Differentiation2

Intracellular3

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Enzyme2

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Exon2

Abstract1

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Membrane3

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Tissues2

Humans1

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