• Micro RNAs (MiRNAs) are endogenous, small RNA molecules that were identified in a wide range of eukaryotes. (usda.gov)
  • About 30% of the human protein-coding genes are negatively regulated by miRNAs which suggests that they have a major role in regulating gene expression. (usda.gov)
  • We now have identified and characterized 54 miRNAs from rainbow trout somatic tissues and profiled their expression patterns. (usda.gov)
  • The majority of the miRNAs showed characteristic tissue-specific expression patterns suggesting potential roles in maintaining tissue identity. (usda.gov)
  • Potential miRNA-target interactions were computationally predicted and single nucleotide polymorphisms (SNPs) were identified in the miRNAs and their target sites in the rainbow trout transcripts. (usda.gov)
  • miRNAs are a family of small non-coding RNAs that emerged as significant and versatile regulators involved in regulation of immune responses. (frontiersin.org)
  • MicroRNAs (miRNAs) are highly conserved, single-stranded, small non-coding RNAs that have been shown to act as essential regulators of gene expression at the posttranscriptional level. (frontiersin.org)
  • miRNAs induce gene degradation and suppression of translation, or both, mainly by imperfect binding to miRNA recognition elements (MREs) within the 3′-untranslated region (UTR) of target mRNAs ( 1 ). (frontiersin.org)
  • microRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression, predominantly through imperfect base pairing with the 3′-untranslated region of target mRNAs ( 5 ). (spandidos-publications.com)
  • There is evidence that clearly demonstrates that, apart from genetic and epigenetic abnormalities, the dysregulation of miRNAs may also contribute to the aberrant activation of oncogenes and the inactivation of tumor suppressor genes in human carcinogenesis ( 9 , 10 ). (spandidos-publications.com)
  • Aberrant expression of miRNAs has been widely reported in human cancers with both up- and downregulation detected in HCC tumor tissue relative to the corresponding normal tissue ( 11 - 13 ). (spandidos-publications.com)
  • miRNAs are notably stable in blood, and their expression patterns appear to be tissue-specific. (spandidos-publications.com)
  • A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
  • Various studies have indicated that microRNAs (miRNAs) are involved in multiple processes of embryo implantation, but the role of miRNAs in endometrial receptivity in patients with recurrent implantation failure (RIF) remains elusive. (biomedcentral.com)
  • MicroRNAs (miRNAs) are an integral part of the post-transcriptional machinery of gene expression and have been implicated in the carcinogenic cascade. (cdc.gov)
  • SNPs in 6 miRNA processing genes and in 78 miRNAs which target genes known to be important in breast cancer among 906 African American (AA) and 653 European American (EA) cases and Author Manuscript controls enrolled in the Women's Circle of Health Study (WCHS). (cdc.gov)
  • We found a number of SNPs in miRNAs and processing genes in association with breast cancer overall or stratified by estrogen receptor (ER) status. (cdc.gov)
  • Background: MicroRNAs (miRNA) are small, highly conserved, non-coding RNAs that regulate gene expression of target mRNAs through cleavage or translational inhibition. (usda.gov)
  • Real-time PCR was used to measure miRNA expression patterns in adult somatic tissues and unfertilized eggs. (usda.gov)
  • Identification of sequence variations in the miRNA -target interactions may be particularly useful for rainbow trout breeding programs. (usda.gov)
  • microRNA (miRNA) expression level alterations between endometrial tissue and endometriotic lesions indicate their involvement in endometriosis pathogenesis. (mdpi.com)
  • These findings suggest miR-486-5p negatively regulates NEK2, which is a critical prognostic indicator of HCC patient survival after liver transplantation. (oncotarget.com)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • Circulating extracellular RNAs (ex-RNAs) are an emerging class of biomarkers with target-organ epigenetic effects. (researchgate.net)
  • Therefore, to improve treatment efficacy, it is necessary to tailor therapies to patient and tumor characteristics, using appropriate molecular targets. (frontiersin.org)
  • The expression of serum miR‑101 in patients with HBV‑related HCC was significantly higher than that in the healthy controls, and this increase correlated with hepatitis B surface antigen positivity, HBV DNA levels and tumor size. (spandidos-publications.com)
  • High NEK2 expression correlated with tumor size, pathological grade and macro- and microvascular invasion. (oncotarget.com)
  • Tumor xenograft data from Balb/c nude mice demonstrated that HCC cells with high NEK2 expression formed larger tumors than those with low NEK2 expression. (oncotarget.com)
  • Incorporated into a protein complex named RISC, microRNAs perform posttranscriptional gene regulation either through perfect binding to a cis-regulatory target site in the 3'UTR that is subsequently cleaved, leading to mRNA degradation, or by imprecise binding preferably of the microRNA 5' end to a target site, leading to possibly reversible repression of protein production. (biomedcentral.com)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • I am molecular and cellular biologist with expertise in virus and microRNAs. (researchgate.net)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • The expression level of miR-30d-5p decreased in the endometria during the implantation window of patients with RIF, whereas the mRNA and protein levels of SOCS1 were significantly higher in the RIF group than in the control group. (biomedcentral.com)
  • On a human dataset revealing fold-change in protein production for five selected microRNAs our method shows superior performance in all classes. (biomedcentral.com)
  • While recent estimates suggest that more than 50% of human protein-coding genes may be regulated by microRNAs and that each microRNA may bind to 300-400 target genes, the latest release of the TarBase database contains information on only 995 human in vivo microRNA-gene interactions involving 103 distinct microRNAs and 825 distinct genes, a far cry from the actual extent of microRNA targeting [ 2 , 4 ]. (biomedcentral.com)
  • Computational prediction of microRNA/gene interactions is a valuable tool for guiding wet-lab experiments, and it remains the only option for systematic genome-wide reconstruction of the complex combinatorial picture of microRNA-mediated target binding. (biomedcentral.com)
  • Experimental detection of microRNA target sites is a costly and time-consuming process. (biomedcentral.com)
  • Long noncoding-RNAs (lncRNAs) are critical regulators of cardiac development as well as pathological hypertrophy and heart failure (HF). (researchgate.net)
  • It is also a challenging task because of the daunting difficulty of distinguishing true microRNA-mRNA hybrids against the noisy background of millions of possible microRNA-gene combinations and, more generally, because the basic mechanisms of microRNA target recognition remain largely unknown. (biomedcentral.com)
  • Extracellular small non-coding RNAs (exRNAs) have been associated with cardiac inflammation and fibrosis and we hypothesized that they are associated with post-MI LVRm phenotypes. (researchgate.net)
  • We estimate that TargetSpy predicts between 26 and 112 functional target sites without a seed match per microRNA that are missed by all other currently available algorithms. (biomedcentral.com)
  • B-cell lymphomas (BCLs) constitute a diverse set of tially identify new functional, diagnostic, and therapeutic tumors, both morphologically and clinically, that are mainly targets. (lu.se)
  • Thus, to maintain a balance between host preservation and inflammatory pathology, the immune response and inflammation need to be tightly regulated. (frontiersin.org)
  • I am very interested in making gene therapies through viral vectors (AAV, lentivirus, adenovirus) available, affordable, and reliable. (researchgate.net)
  • In the last decade, diagnostic and prognostic evaluation has been facilitated by global gene expression profiling (GEP), provid- ing a new powerful means for the classification, prediction of survival, and response to treatment of lym- phomas. (lu.se)
  • Our current knowledge about the function of specific microRNAs, their targeted messenger RNAs, and the exact location of binding sites is limited. (biomedcentral.com)
  • (12 ). However, the versatility of the system allows the use of any microRNA of choice, including neuron-specific microRNAs ( 14 ). (lu.se)
  • The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treat- ment. (lu.se)
  • When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
  • While long noncoding RNAs (lncRNAs) are important i. (researchgate.net)
  • While long noncoding RNAs (lncRNAs) are important in cardiac development and disease, their role in physiol. (researchgate.net)
  • We found that lipopolysaccharide and Vibrio anguillarum significantly upregulated the expression of fish miR-21. (frontiersin.org)
  • Analysis of multiple microarrays in the Oncomine database revealed that NEK2 expression was higher in HCC tissues than adjacent normal liver tissues. (oncotarget.com)
  • Notably, serum miR‑101 levels were found to have an inverse correlation with tissue miR‑101 expression levels. (spandidos-publications.com)
  • Recently however, it has been shown that this requirement might be too stringent, leading to a substantial number of missed target sites. (biomedcentral.com)
  • Only a few algorithms can predict target sites without demanding a seed match and TargetSpy demonstrates a substantial improvement in prediction accuracy in that class. (biomedcentral.com)
  • Moreover, we have demonstrated that the application of machine learning techniques in combination with upcoming deep sequencing data results in a powerful microRNA target site prediction tool http://www.targetspy.org . (biomedcentral.com)
  • In addition, a significant decrease in LIF and p-STAT3 expression was observed during the implantation window in patients with RIF. (biomedcentral.com)
  • Quantitative reverse transcription-polymerase chain reaction and western blotting were used to investigate the expression levels of miR-30d-5p, SOCS1, and the LIF pathway. (biomedcentral.com)
  • We also demonstrated an inverse relationship between miR-486-5p and NEK2 expression in HCC patients. (oncotarget.com)
  • A recent study of the disparity in breast cancer mortality between AA and EA women concluded that differences in mortality are driven by higher hazard rates of breast cancer death in AA women, irrespective of ER expression [9]. (cdc.gov)
  • We analyzed the relationship between differential expression of NEK2 and hepatocellular carcinoma (HCC) patient outcomes after liver transplants. (oncotarget.com)