• Moderna said via Twitter on July 8 that it completed enrollment of its Phase II trial of mRNA-1273 ( NCT04405076 ). (genengnews.com)
  • Six days earlier, Moderna said via Twitter on July 2 that its planned 30,000-patient Phase III trial of mRNA-1273 is still expected to begin in July, "and we expect to be the first to start a Phase III trial," despite changes to the trial's protocol following talks with the NIH that pushed back the start from an expected July 9 launch . (genengnews.com)
  • A day earlier, Moderna said it would partner for large-scale, commercial fill-finish manufacturing of mRNA-1273 with Catalent at its biologics facility in Bloomington, IN. (genengnews.com)
  • The companies are in talks to secure fill-finish capacity for continued production of hundreds of millions of additional doses, Moderna said, while Catalent also agreed to provide clinical supply services from its facilities in Philadelphia-including packaging and labeling, as well as storage and distribution to support Moderna's Phase III clinical study for mRNA-1273. (genengnews.com)
  • Moderna on June 12 said it finalized the study protocol for its planned Phase III trial of mRNA-1273, based on feedback from the FDA. (genengnews.com)
  • When you have the right sequence … you will get neutralizing antibodies," Moderna CEO Stephane Bancel told CNBC, predicting mRNA-1273 had an 80% to 90% chance of success. (genengnews.com)
  • Earlier in June, the Biomedical Advanced Research and Development Authority (BARDA) said it was expanding its collaboration with Moderna in order to increase the domestic manufacturing capacity of mRNA-1273 by accelerating manufacturing scale-up. (genengnews.com)
  • The expansion is expected to increase BARDA's commitment made in April of up to $483 million to Moderna toward supporting development and manufacturing of mRNA-1273 through FDA licensure. (genengnews.com)
  • MRNA - Moderna, Inc. (yahoo.com)
  • Moderna, Inc., (MRNA) a clinical stage biotechnology company pioneering messenger RNA (mRNA) therapeutics and vaccines to create a new generation of transformative medicines for patients, today announced that it will host its annual Science Day for analysts and investors on Tuesday, May 7, 2019 at 8:00 a.m. (yahoo.com)
  • Investors need to pay close attention to Moderna (MRNA) stock based on the movements in the options market lately. (yahoo.com)
  • Moderna, Inc., (MRNA) a clinical stage biotechnology company pioneering messenger RNA (mRNA) therapeutics and vaccines to create a new generation of transformative medicines for patients, today announced that Tal Zaks, M.D., Ph.D., Moderna's Chief Medical Officer, will participate in a fireside chat at the 18th Annual Needham Healthcare Conference on Wednesday, April 10, 2019 at 11:20 a.m. (yahoo.com)
  • How Much Of Moderna, Inc. (NASDAQ:MRNA) Do Insiders Own? (yahoo.com)
  • If you want to know who really controls Moderna, Inc. (NASDAQ:MRNA), then you'll have to look at the makeup of its share registry. (yahoo.com)
  • The vaccine is called mRNA-1273 and was developed by the NIAID scientists and their collaborators at the biotechnology company Moderna, Inc., based in Cambridge, Massachusetts. (indiatimes.com)
  • Moderna is supposed to sidestep that expensive and time-consuming process, by synthesizing mRNAs, injecting them, and letting them carry the desired genetic code into cells so the body itself acts like a drugmaking factory, churning out the needed protein in its proper shape and place. (xconomy.com)
  • All told, those moves have left Moderna with $413 million in the bank right now, Bancel says, to plow forward with its audacious plan to make injectable mRNA molecules that trigger the production of protein drugs within the body -a completely new drug modality, if it works in humans. (xconomy.com)
  • Moderna has been using its status as a private company to keep many of the details behind its technology close to the vest, such as how it can deliver these mRNA molecules into the body without setting off alarm bells in the immune system. (xconomy.com)
  • That's all before starting its first clinical trial, though CEO Stephane Bancel has said that the Moderna expects to have several mRNA drugs in clinical testing by the end of 2016. (xconomy.com)
  • Bancel says by the end of 2016, several mRNA drugs should be in clinical testing, and that Moderna would start sharing its data with the scientific community. (xconomy.com)
  • Moderna itself won't develop and sell the mRNA drugs it creates, but it also won't have to do all the work-and carry all the risk of potential failure-on its own. (xconomy.com)
  • In 2014, Alexion teamed up with Moderna in a $100m agreement to discover and develop messenger RNA (mRNA) therapeutics to treat rare diseases. (outsourcing-pharma.com)
  • This mRNA program for rare disease is part of Alexion's collaboration with Moderna [and] Alexion wanted access to our industry-leading LNP technology and know-how. (outsourcing-pharma.com)
  • Given that the same transcriptional unit can potentially give rise to both miRNA and mRNA transcripts raises the intriguing question of the level of interaction between these processes. (hindawi.com)
  • During nuclear maturation of primary transcripts, both ends of mRNA are post‐transcriptionally modified through the addition of a 7‐methyl‐guanosine cap structure at the 5′ end and a polyadenosine tail at the 3′ end. (els.net)
  • Containing coding sequences called transcripts, mRNA aids in th. (reference.com)
  • Identity of the first 10 nucleotides in B. subtilis mRNA transcripts. (esrf.eu)
  • When one looks at primary transcripts in B. subtilis , it is clear that there is a counter-selection for mRNAs with G in the second position ( Figure 69 ). (esrf.eu)
  • This innovative technology offers protection of full-length poly(A) mRNA transcripts present in stored samples of purified total RNA. (sigmaaldrich.com)
  • Results show successful preservation of full-length poly(A) mRNA transcripts following 21 month dry storage in RNAstable. (sigmaaldrich.com)
  • The A allele resulted in an increased production of mature transcripts with a long 5′ leader in several cell lines, and the extended mRNAs generated more proinsulin in culture supernatants than natural transcripts. (diabetesjournals.org)
  • As intron 1 separates noncoding and coding exons, differential utilization of its 3′ splice site by the uridine- and adenosine-containing pre-mRNAs would be predicted to result in distinct representation of mature transcripts with short and long 5′ untranslated regions (UTRs) with potential effects on translation. (diabetesjournals.org)
  • In fact, up to 70% of the mRNA mass is from globin transcripts. (bio-medicine.org)
  • The kit employs a novel hybridization capture technology (see "Simple, Effective Globin mRNA Reduction Protocol" at right) that uses the power of biotin/streptavidin binding and the specificity of nucleic acid hybridization to effectively remove 9099.5% globin transcripts from blood total RNA (Figure 1). (bio-medicine.org)
  • To further test the specificity of globin mRNA reduction, synthetic globin transcripts were added to HeLa cell RNA, and samples were processed or left unprocessed with the GLOBINclear Kit (Figure 2). (bio-medicine.org)
  • With the removal of globin transcripts, the enriched RNA samples become superior templates for RNA expression profiling because the presence of globin mRNA is no longer a factor in decreasing the sensitivity of gene detection on microarrays (Figure 2). (bio-medicine.org)
  • In animal cells, these small untranslated RNAs repress gene expression by annealing to mRNAs to which they are partially complementary. (pnas.org)
  • Mitochondrial extracts from Trypanosoma brucei were fractionated on 10-30% glycerol gradients and assayed for RNAs and activities potentially involved in editing, including pre-edited mRNA, guide RNA (gRNA), endonuclease, terminal uridylyltransferase (TUTase), RNA ligase and gRNA-mRNA chimera-forming activities. (nih.gov)
  • Cis -regulatory elements play a number of important roles in determining the fate of messenger RNAs (mRNAs). (springer.com)
  • Cuscuta pentagona (dodder) is a parasitic plant that forms symplastic connections with its hosts and takes up host messenger RNAs (mRNAs). (sciencemag.org)
  • Human acetyl-coA cholesterol acyltransferase (ACAT1) gene produces a chimeric mRNA through the interchromosomal processing of two discontinuous RNAs transcribed from chromosomes 1 and 7. (wikipedia.org)
  • The last chapter highlights how the discovery of disease-causing defects (or modifications) in mRNA can provide a variety of therapeutic targets that can be used for the development of new RNA-based therapeutics. (springer.com)
  • Indeed, one of Moderna's nascent startups, Valera , is developing mRNA vaccines and therapeutics for infectious diseases. (xconomy.com)
  • Establishing Onkaido as a standalone unit will accelerate the development of mRNA therapeutics for oncology, it adds. (pharmatimes.com)
  • Earlier this week, Alexion said it was paying $100 million upfront to purchase ten product options to Moderna's mRNA therapeutics platform. (pharmatimes.com)
  • By combining Merck's strength in vaccine and antiviral therapeutic development with Moderna's mRNA Therapeutics technology we are well positioned to develop differentiated candidates with the potential to provide meaningful benefit to patients," said Roger Perlmutter, president of Merck Research Laboratories, commenting on the deal. (pharmatimes.com)
  • The article titled, Leveraging Rational Protein Engineering to Improve mRNA Therapeutics, is co-authored by Romesh Subramanian and colleagues from Alexion, New Haven, Connecticut, USA. (siasat.com)
  • Moderna's Science Day will feature presentations from Stephen Hoge M.D., president and Melissa Moore Ph.D., chief scientific officer of Moderna's mRNA Research Platform with a focus on the Company's newest advances from its commitment to basic and applied sciences. (yahoo.com)
  • Whereas Moderna's initial plan was to create both a messenger RNA (mRNA) platform and develop its own mRNA drugs when it started up a few years ago, it has quietly changed that focus. (xconomy.com)
  • Although initial reports suggested that down-regulation by partially complementary miRNAs was due entirely to decreased translation ( 2 - 4 ), recent studies have indicated that miRNAs can also reduce the cellular concentration of the mRNAs that they regulate, both in vitro and in vivo ( 12 - 16 ). (pnas.org)
  • mRNA untranslated regions - UTRs - are involved in the post‐transcriptional regulation of gene expression by modulating the mRNA stability, nucleocytoplasm transport, subcellular localisation and translation efficiency, thus allowing a fine control of the protein product. (els.net)
  • Fabian MR, Sonenberg N and Filipowicz W (2010) Regulation of mRNA translation and stability by microRNAs. (els.net)
  • Axonal mRNA transport and localized translational regulation of kappa-opioid receptor in primary neurons of dorsal root ganglia. (mendeley.com)
  • kappa-opioid receptor (KOR) is detected pre- and postsynaptically, but the subcellular localization, translation, and regulation of kor mRNA in presynaptic compartments of sensory neurons remain elusive. (mendeley.com)
  • This book provides a collection of novel studies and hypotheses aimed to define the pathophysiological consequences of altered mRNA metabolism events in human cells, and is written for a wide spectrum of readers in the field of gene expression regulation. (springer.com)
  • This report describes the development and application of real time RT-PCR to measure the expression of several important elk ( Cervus elaphus ) cytokine mRNAs, including interleukin (IL)-2, IL-4, IL-10, IL-12p40, interferon-gamma, tumor necrosis factor (TNF)- α, and the enzyme-inducible nitric oxide synthase, all of which are involved in immune responses and regulation. (bioone.org)
  • In addition, FMRP, FXR1, and FXR2 are components of stress granules (SG) and have been implicated in the translational regulation of mRNAs (6). (cellsignal.com)
  • Reed R and Hurt E (2002) A conserved mRNA export machinery coupled to pre‐mRNA splicing. (els.net)
  • Mignone F, Gissi C, Liuni S and Pesole G (2002) Untranslated regions of mRNAs. (els.net)
  • In nerve cells, which form cytoplasmic processes that can be very long, many neuronal mRNAs must be conveyed to the sites of action of their protein products to ensure that the correct intercellular connections can be established. (eurekalert.org)
  • The MS2-fused GFP tracked kor mRNA transport from DRG neuronal soma to axons, requiring its 5' and 3' UTRs. (mendeley.com)
  • To date there has been no direct evidence that neuronal derived mRNAs are transported into and translated within mature PNS or CNS axons in vivo . (jneurosci.org)
  • Conversely, although poly(A) removal appears to be a key step in miRNA-mediated mRNA decay, a poly(A) tail is not required for translational repression by miRNAs. (pnas.org)
  • UTRs are miRNA targets to repress translation or destabilise mRNA. (els.net)
  • Subcellular localisation of mRNA is often mediated by miRNA elements located in the 3′ UTR. (els.net)
  • When performing miRNA quantification, your qPCR mRNA experiments' success can be impacted by cost, sensitivity, reproducibility, low multiplexing level and difficulty in designing or finding the right assay. (qiagen.com)
  • Novel lipid nanoparticle (LNP)-encapsulated mRNA vaccine encoding for a prefusion stabilized form of the Spike (S) protein. (genengnews.com)
  • World Health Organization (WHO) Chief Scientist Soumya Swaminathan, MD, MBBS, told reporters June 26 she considered mRNA-1273 "not far behind" what she called "probably the leading candidate" among COVID-19 vaccine candidates in development worldwide, AZD1222 by AstraZeneca, University of Oxford and Vaccitech. (genengnews.com)
  • mRNA-1273 will be the first COVID-19 product to be shifted for advanced development from NIH to BARDA, which said expansion activities are expected to begin with delivering material for manufacturing "mid-year," with the vaccine available as early as the end of 2020. (genengnews.com)
  • And today CureVac is announcing a new partnership, with the non-profit International AIDS Vaccine Initiative , to try and develop an mRNA-based AIDS vaccine. (xconomy.com)
  • Together, these broad technology patents strengthen CureVac's industry-leading position in the mRNA space as the Company develops preventative vaccine candidates for severe infectious diseases, such as RSV and influenza. (globenewswire.com)
  • Messenger RNA is demonstrating success in vaccine manufacturing and CureVac is giving it the place to do so with the development of a prophylactic vaccine with mRNA at its core. (in-pharmatechnologist.com)
  • CureVac, a biopharmaceutical company, recently announced it began a Phase I dose-escalation clinical trial for its mRNA-based rabies vaccine and stated that mRNA can " simplify" ​ vaccine manufacturing. (in-pharmatechnologist.com)
  • Big pharma companies like Pfizer ​ have signed hundred-million dollar deals into mRNA-based vaccine research. (in-pharmatechnologist.com)
  • Manufacturers like BioNTech, Pfizer's partner in mRNA vaccine research, said that mRNA vaccine manufacturing can be more cost-effective as the vaccines do not require the same amount of quality control as typical virus-based vaccines. (in-pharmatechnologist.com)
  • mRNA vaccine manufacturing isn't limited to these companies, AstraZeneca ​, eTheRNA ​, and Sanofi ​ have all invested into the potential 'game-changer' ​ technology. (in-pharmatechnologist.com)
  • In-PharmaTechnologist ( IPT ​) spoke with Dan Menichella ( DM ​), CEO of CureVac, about its prophylactic rabies vaccine (CV7202) and where mRNA exists in the vaccine manufacturing sector. (in-pharmatechnologist.com)
  • IPT: What role does mRNA technology play in today's vaccine industry? (in-pharmatechnologist.com)
  • mRNA vaccines are a promising alternative to conventional vaccine approaches because of their high potency, potential for rapid development and production, and low cost to manufacture. (in-pharmatechnologist.com)
  • mRNA vaccines have the potential to significantly simplify vaccine manufacturing and product release. (in-pharmatechnologist.com)
  • We believe mRNA technology has the potential to be disruptive and replace a significant part of traditional vaccine manufacturing since it can be easier and faster, resulting in reduced costs in the long -term. (in-pharmatechnologist.com)
  • IPT: In the future can mRNA vaccine technology be applied to different uses outside the prophylactic use? (in-pharmatechnologist.com)
  • The lipid nanoparticle (LNP) formulation we are using for our rabies vaccine is also applicable to other local administrations of mRNA requiring a local immune activation, like therapeutic cancer vaccines or intratumoral mRNA treatments. (in-pharmatechnologist.com)
  • With our mRNA-based vaccine, we are testing either a one-shot dose for the travelers market and a two-shot dose for post exposure. (in-pharmatechnologist.com)
  • In a pre-clinical study, a single intramuscular vaccination of non-human primates with this mRNA vaccine induced antibody titers well above the World Health Organization's (WHO) [guidance of] titer of 0.5 IU/ml, which remained stable during an observation period of up to nine months. (in-pharmatechnologist.com)
  • A clinical update on COVID-19, phase I preliminary results of the SARS-CoV-2 mRNA vaccine, and factors associated with death in 17 million patients. (asm.org)
  • Daniel Griffin provides a clinical update on COVID-19, then we discuss phase I preliminary results of the SARS-CoV-2 mRNA vaccine, factors associated with death in 17 million patients, and answer listener questions. (asm.org)
  • The eukaryotic gene expression pathway involves a number of interlinked steps, with messenger RNA (mRNA) being the key intermediate. (springer.com)
  • Although eukaryotic cells have evolved elaborate mRNA quality control mechanisms that ensure the fidelity of gene expression, some defects are not detected, thus affecting mRNA metabolism. (springer.com)
  • The shorter the half-life of its mRNA, the more rapidly a cell can alter its gene expression programme in response to changes in the environment. (esrf.eu)
  • the isolation of mRNA for GeneChips arrays (Affymetrix Inc.) (1,2), for Gene Expression Microarray™ (Incyte Genomics Inc) (3, 4), and for different types of self-spotted arrays (5-11). (thermofisher.com)
  • The relationship between gene expression measured at the mRNA level and the corresponding protein level is not well characterized in human cancer. (mcponline.org)
  • In human lung cancer, however, little is currently understood regarding the relationship between gene expression as determined by measuring mRNA levels and the corresponding abundance of the protein products. (mcponline.org)
  • Karen Slobod, a former Novartis Vaccines executive, will head the new outpost, whose focus will be developing mRNA-based prophylactic vaccines for things like HIV, respiratory syncytial virus, and the flu. (xconomy.com)
  • In a strong show of confidence in the technology, Merck said it will pay the biotech $50 million upfront in cash coupled with a $50 million equity investment, to bind a three-year research collaboration focused on the development of new mRNA-based treatments and vaccines against four undisclosed viruses. (pharmatimes.com)
  • The breakthrough of vaccines ended epidemics and prevented outbreaks - a powerful technology that is gaining breakthrough status with mRNA leading the charge. (in-pharmatechnologist.com)
  • Vaccines with mRNA technology are also able to rapidly adjust to changing viruses allowing for greater protection, according to BioNTech. (in-pharmatechnologist.com)
  • In addition, mRNA offers a unique potential for the development of multivalent vaccines targeting a high number of antigens. (in-pharmatechnologist.com)
  • IPT: How does manufacturing differ for the creation of vaccines using mRNA as opposed to traditional methods? (in-pharmatechnologist.com)
  • mRNA-based vaccines, for different disease targets, can be manufactured in a single multiproduct facility using standardized production methods. (in-pharmatechnologist.com)
  • Treatment of the human bronchial epithelial cell line, BEAS-2B, with arsenic(III) chloride (As3+) resulted in a significant increase in GADD45alpha protein and mRNA. (cdc.gov)
  • The Zaher lab has found that that oxidative damage to a single base (bold X) on a messenger RNA (the jagged ribbon) can jam the cellular nanomachine (green) that translates the mRNA into protein (ribbon into beads). (eurekalert.org)
  • Studies demonstrate that mRNA is stabilized for up to 21 months at room temperature and even at elevated temperatures (+50°C) for extended time periods. (sigmaaldrich.com)
  • Our results demonstrate that mRNA delivery is a potential approach for cardiomyocyte generation. (dovepress.com)
  • Andreassi C and Riccio A (2009) To localize or not to localize: mRNA fate is in 3′ UTR ends. (els.net)
  • mRNAs localize to specific regions within neurons, where they can be translated to quickly generate large amounts of a protein in the place where it is needed. (rupress.org)
  • mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. (neb.com)
  • The second nucleotide of this mRNA is naturally a G-residue, consistent with the enzyme's nucleotide preference in this position. (esrf.eu)
  • These results indicate that −23 Hph I and IVS-69 are the most important INS variants affecting pre-mRNA splicing and suggest that −23 Hph I+/− is a common functional single nucleotide polymorphism at IDDM2 . (diabetesjournals.org)
  • Since uridine is the preferred PPT nucleotide in pre-mRNA ( 9 ), the A allele, which reduces the Shapiro-Senapathy matrix score and other splicing prediction scores for the acceptor splice site of intron 1 (online appendix Table 1 , available at http://diabetes.diabetesjournals.org ), is likely to weaken efficient splicing of this intron. (diabetesjournals.org)
  • Nucleotide sequencing of the resulting mRNAs identified six alternatively spliced isoforms designated 1-6 ( Fig. 1 B ). Isoforms 2, 4, and 6 have correctly spliced the 3′ splice site of intron 2, whereas isoforms 1, 3, and 5 lack the 5′ part of exon 3 due to a cryptic 3′ splice site activation ( Fig. 1 A and B ), confirming an earlier observation ( 10 ). (diabetesjournals.org)
  • EXOSC8 mutations alter mRNA metab. (mendeley.com)
  • These modifications can alter mRNA structure, being able to modulate different steps of the mRNA metabolism that can be associated with various human diseases, such as systemic lupus erythematosus and cancer. (springer.com)
  • Axonal mRNA transport and localiz. (mendeley.com)
  • Axonal mRNA transport is robust in cultured neurons but there has been limited evidence for this in vivo . (jneurosci.org)
  • Here, we have used a transgenic approach to directly test for axonal mRNA transport in vivo . (jneurosci.org)
  • When a single mRNA aligned in multiple places, the alignment having the highest base identity was found. (ucsc.edu)
  • Upon stimulation, Pin1 becomes dephosphorylated and AUF1 subunits are phosphorylated, leading to the release of the AUF1 complex from the mRNA and the binding of the hnRNP C, which promotes mRNA stability and allows increased production of the GM-CSF protein. (sciencemag.org)
  • Our data indicate that the 3′-UTR of β-actin is sufficient to target a reporter mRNA for transport into distal axons in vivo . (jneurosci.org)
  • Here, we develop a modular, synthetic mRNA-based approach to express neutralizing antibodies directly in the lung via aerosol, to prevent RSV infections. (nature.com)
  • First, we expressed whole palivizumab (secreted, termed sPali) in the lung via synthetic mRNA delivery by intratracheal aerosol. (nature.com)
  • The therapeutic effect is long term, as shown by markedly improved survival following myocardial infarction with a single administration of the synthetic mRNA when given within 48 hours after the heart attack. (medindia.net)
  • In particular, it will become of interest to engineer new device technology to deliver the synthetic mRNA via conventional catheter technology. (medindia.net)
  • Organelles called ribosomes are the site of translation, the process by which mRNA is decoded into a corresponding protein. (wisegeek.com)
  • But just as cars sometimes drive through stop signals on the road, ribosomes sometimes "read through" an mRNA's stop codon, staying attached to the mRNA even though they have strayed into the mRNA's 3′ noncoding region. (genengnews.com)
  • A small proportion of ribosomes translating AMD1 mRNA stochastically read through the stop codon of the main coding region," wrote the article's authors. (genengnews.com)
  • The team of scientists, led by Dmitry Andreev, Ph.D., a senior research associate Lomonosov Moscow State University, found out that approximately one in 60 ribosomes moves through the stop codon of the AMD1 mRNA, but after some time it stalls on the "wrong" sequence. (genengnews.com)
  • The chemical reactions and pathways resulting in the breakdown of histone messenger RNA (mRNA). (yeastgenome.org)
  • Messenger RNA (mRNA) is made up of four molecular building blocks: adenosine, cytidine, guanosine and uridine, which can be modified by the attachment of chemical tag. (idw-online.de)
  • To coax the heart to make the VEGFA, the investigators in the Nature Biotechnology study used new technology where synthetic messenger RNA (mRNA) that encodes VEGFA is injected into the muscle cell. (medindia.net)
  • Washington: Rational protein engineering can improve the effectiveness of messenger RNA (mRNA) therapies, a new research has revealed. (siasat.com)
  • This process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). (wikipathways.org)
  • TÜBINGEN, Germany and BOSTON, Jan. 08, 2019 (GLOBE NEWSWIRE) -- CureVac AG, a fully integrated biopharmaceutical company pioneering the field of mRNA-based drugs, announced that the U.S. Patent and Trademark Office (USPTO) has granted a fundamental patent for the use of mRNA encoding the Respiratory Syncytial Virus (RSV) F-protein for vaccination of infants up to two years of age (US Patent No. 10,172,935). (globenewswire.com)
  • b) After splicing, the TREX complex is recruited to a region near the 5′ end of the mRNA via an interaction between CBP80 and Aly. (els.net)
  • Huang Y and Steitz JA (2001) Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA. (els.net)
  • 2005) Recruitment of the human TREX complex to mRNA during splicing. (els.net)
  • Strasser K and Hurt E (2001) Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p. (els.net)
  • During the pre-mRNA splicing process, introns are removed and exons are joined in order to generate the mature mRNA [ 1 - 3 ]. (hindawi.com)
  • Alternative splicing can result in mRNAs encoding the same protein under the control of different UTRs. (els.net)
  • It is also found in pre-mRNA-splicing factor 18 [ PMID: 9000057 ]. (ebi.ac.uk)
  • A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor. (ebi.ac.uk)
  • Here, we report that dysregulation of exon junction complex-driven (EJC-driven) mRNA splicing confers hyperpinocytosis to macrophages during atherogenesis. (jci.org)
  • In prokaryotes , mRNA is produced by splicing a large primary transcript from a DNA sequence . (biology-online.org)
  • We examined whether INS polymorphisms affect pre-mRNA splicing and proinsulin secretion using minigene reporter assays. (diabetesjournals.org)
  • hnRNP A1 regulates the alternative splicing of c-Src and c-H-Ras (2,3) and modifies initiation of translation of the fibroblast growth factor 2 mRNA (4). (cellsignal.com)
  • Exons are regularly alternatively spliced, meaning that they are either included or excluded from the final mature mRNA transcript. (hindawi.com)
  • Six transcript variants of mlf1 mRNA, with more than 2000 bps, are analyzed by focusing on the autocorrelation of each distribution. (hindawi.com)
  • The mRNA assays have exceptional sensitivity and specificity using short, LNA-enhanced primers and are optimized to eliminate nonspecific amplification. (qiagen.com)
  • In addition to repressing translation, miRNAs can down-regulate the concentration of mRNAs that contain elements to which they are imperfectly complementary. (pnas.org)
  • UTRs regulate mRNA stability, nucleocytoplasm transport, subcellular localisation and translation efficiency. (els.net)
  • Export of mRNA involves a specific cellular machinery that is highly conserved from yeast to humans. (els.net)
  • 1999) The Mex67p‐mediated nuclear mRNA export pathway is conserved from yeast to human. (els.net)
  • Dierk Niessing, a professor at LMU's Biomedical Center and leader of a research group in the Institute of Structural Biology at the Helmholtz Zentrum München, has now characterized the structure of a macromolecular complex involved in the transport of mRNAs in yeast cells. (eurekalert.org)
  • P. Chartrand, X.H. Meng, S. Huttelmaier, D. Donato, R.H. Singer: Asymmetric sorting of ash1p in yeast results from inhibition of translation by localization elements in the mRNA, Mol. (springer.com)
  • Recent advances in the systematic comparison of different method variants in yeast have permitted the identification of the least invasive methodologies that reflect half-lives the most faithfully, which is expected to open the way for a consistent quantitative analysis of the determinants of mRNA stability. (mdpi.com)
  • This mechanism emerged from a study of the mRNA that is transcribed from the AMD1 gene, which encodes the protein adenosylmethionine decarboxylase 1 (AdoMetDC). (genengnews.com)
  • At this stage par-1 is required for polarisation of the oocyte microtubule network, and for localisation of oskar mRNA, which encodes the posterior determinant of the fly. (biologists.org)
  • CureVac, a Germany-based mRNA company spun out of the University of Tubigen 15 years ago, said this morning that it's opening up a U.S. hub in Cambridge . (xconomy.com)
  • Expansion into the U.S. is an important step in CureVac's corporate and clinical growth as it will enable us to capitalize on the numerous research and business opportunities related to our industry-leading mRNA technology platform," CureVac co-founder and CEO Ingmar Hoerr said in a statement. (xconomy.com)
  • For such optimized mRNA constructs, CureVac already holds a U.S. patent that supplements CureVac's patent portfolio regarding RSV vaccination. (globenewswire.com)
  • The hybridisation kinetics between mRNA and the Dynabeads® are not only fast and efficient, but also comparable to that of free solution hybridisation. (thermofisher.com)
  • It might be that putative structural elements in the PrP mRNA influence the kinetics of sequential folding of the protein during translation, and that the process of infection acts via those structural elements. (cyber-dyne.com)
  • Pharmacological inhibition of Pin1 with juglone prevented increased mRNA abundance in and secretion of GM-CSF from isolated eosinophils in response to hyaluronic acid. (sciencemag.org)
  • The mRNA and tRNA temporarily bind at the codon site, allowing the amino acid on the tRNA to get close enough to the previous amino acid to form a peptide bond. (wisegeek.com)
  • This primer is used for the priming and sequencing of mRNA adjacent to the 3'-poly A tail (1). (neb.com)
  • The precursor mRNA is transcribed from DNA, processed by removal of introns and addition of the cap structure and the poly(A) tail. (springer.com)
  • Both mRNAs are attached with poly A following this overlap region. (bio.net)
  • RNAstable protects poly(A) mRNA. (sigmaaldrich.com)