• To clarify functions of the Mre11/Rad50 (MR) complex in DNA double-strand break repair, we report Pyrococcus furiosus Mre11 crystal structures, revealing a protein phosphatase-like, dimanganese binding domain capped by a unique domain controlling active site access. (rcsb.org)
  • Furthermore, the structure of the P. furiosus Rad50 ABC-ATPase with its adjacent coiled-coil defines a compact Mre11/Rad50-ATPase complex and suggests that Rad50-ATP-driven conformational switching directly controls the Mre11 exonuclease. (rcsb.org)
  • Electron microscopy, small angle X-ray scattering, and ultracentrifugation data of human and P. furiosus MR reveal a dual functional complex consisting of a (Mre11)2/(Rad50)2 heterotetrameric DNA processing head and a double coiled-coil linker. (rcsb.org)
  • The Mre11/Rad50/Nbs1 complex is known to be responsible for the resection of DSB to ssDNA. (dartmouth.edu)
  • Consistent with this, PARP-1 activity increases the rate of recruitment of the MRE11-RAD50-NBS1 (MRN) complex 10 and stimulates Ku binding in Dictyostelium 22 . (nature.com)
  • The levels of Nibrin (NBN) and MRE11, members of the MRN complex (MRE11/Rad50/NBN) responsible for DSB recognition, were significantly down-regulated. (biomedcentral.com)
  • The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. (origene.com)
  • The Mre11 complex, composed of RAD50, NBS1 and MRE11, has an essential role in the maintenance of genomic integrity and preventing cells from malignancy. (lu.se)
  • These structures unify Mre11's multiple nuclease activities in a single endo/exonuclease mechanism and reveal eukaryotic macromolecular interaction sites by mapping human and yeast Mre11 mutations. (rcsb.org)
  • MRE11 Polyclonal Antibody detects endogenous levels of MRE11 protein. (abbkine.com)
  • Ataxialike disorder (ATLD) syndrome involves a mutation in meiotic recombination 11 homolog (MRE11). (medscape.com)
  • Here we report the association of three Mre11 complex mutations with hereditary breast cancer susceptibility, studied by using a case-control design with 317 consecutive, newly diagnosed Northern Finnish breast cancer patients and 1000 geographically matched healthy controls (P = 0.0004). (lu.se)
  • In vitro, DYNLL1 binds directly to MRE11 to limit its end-resection activity. (broadinstitute.org)
  • However, we show that inhibition of the Mre11 nuclease activity leads, not only to a decrease in the amount of ssDNA following Chk1 inhibition, but also inhibits the formation of DSB, suggesting that DSB are a consequence of ssDNA formation. (dartmouth.edu)
  • Active Motif's MRE11 antibody (pAb) was raised in a Rabbit host. (mybio.ie)
  • Belongs to the mre11/rad32 family. (lu.se)
  • DYNLL1 binds to MRE11 to limit DNA end resection in BRCA1-deficient cells. (broadinstitute.org)
  • The Mre11 Nuclease is Critical for the Sensitivity of Cells to Chk1 In" by Ruth Thompson, Ryan Montano et al. (dartmouth.edu)
  • These findings were corroborated by the discovery that Mre11-deficient ATLD1 cells are highly resistant to MK-8776 and form neither ssDNA nor DSB following treatment. (dartmouth.edu)
  • However, once complimented with exogenous Mre11, the cells accumulate both ssDNA and DSB when incubated with MK-8776. (dartmouth.edu)
  • The results highlight a novel role for Mre11 in the production of DSB and may help define which tumors are more sensitive to MK-8776 alone or in combination with DNA damaging agents. (dartmouth.edu)
  • Saccharomyces cerevisiae RAD50 and MRE11 genes are required for the nucleolytic processing of DNA double-strand breaks. (nebraska.edu)
  • We have overexpressed Rad50 and Mre11 in yeast cells and purified them to near homogeneity. (nebraska.edu)
  • Consistent with the genetic data, we show that the purified Rad50 and Mre11 proteins form a stable complex. (nebraska.edu)
  • In the Rad50-Mre11 complex, the protein components exist in equimolar amounts. (nebraska.edu)
  • The addition of Rad50 does not significantly alter the exonucleolytic function of Mre11. (nebraska.edu)
  • Using homopolymeric oligonucleotide-based substrates, we show that the exonuclease activity of Mre11 and Rad50-Mre11 is enhanced for substrates with duplex DNA ends. (nebraska.edu)
  • These endonuclease activities of Mre11 are enhanced markedly by Rad50 but only in the presence of ATP. (nebraska.edu)
  • Trujillo, KM & Sung, P 2001, ' DNA Structure-specific Nuclease Activities in the Saccharomyces cerevisiae Rad50-Mre11 Complex ', Journal of Biological Chemistry , vol. 276, no. 38, pp. 35458-35464. (nebraska.edu)
  • Mre11-Rad50-Nbs1 (MRN) complex involvement in nonhomologous end joining (NHEJ) is controversial. (mdc-berlin.de)
  • In vertebrates, Mre11, Rad50, and Nbs1 are essential genes, and studies have been limited to cells carrying hypomorphic mutations in Mre11 or Nbs1, which still perform several MRN complex-associated activities. (mdc-berlin.de)
  • Here, we find that p97 physically and functionally interacts with the MRE11-RAD50-NBS1 (MRN) complex on chromatin and that inactivation of p97 blocks the disassembly of the MRN complex from the sites of DNA damage upon ionizing radiation (IR). (ox.ac.uk)
  • Protein Mre11, u okviru kompleksa Mre11-Rad50-Nbs1/Xrs2 (MRN/X), ima važnu ulogu u popravcima dvolančanih lomova DNA svih do sada istraživanih organizama, od bakterija do čovjeka. (unist.hr)
  • Background: MRE11 Antibody: MRE11 is involved in the repair of DNA double strand breaks as part of a complex that includes the Rad50 and NBS1 protein and is thought to act in the same pathway as the A-T mutated (ATM) protein. (hatinhibitor.com)
  • Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks. (nih.gov)
  • An interaction between RPA and Mre11 was demonstrated by co-immunoprecipitation of both protein complexes with anti-Mre11, anti-Rad50, anti-NBS1, or anti-RPA antibodies. (nih.gov)
  • 5. MRE11-RAD50-NBS1 complex dictates DNA repair independent of H2AX. (nih.gov)
  • 6. NBS1 promotes the endonuclease activity of the MRE11-RAD50 complex by sensing CtIP phosphorylation. (nih.gov)
  • 7. Deubiquitinase USP2 stabilizes the MRE11-RAD50-NBS1 complex at DNA double-strand break sites by counteracting the ubiquitination of NBS1. (nih.gov)
  • 13. Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair. (nih.gov)
  • 17. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex. (nih.gov)
  • 18. Nbs1 Converts the Human Mre11/Rad50 Nuclease Complex into an Endo/Exonuclease Machine Specific for Protein-DNA Adducts. (nih.gov)
  • 20. Stable maintenance of the Mre11-Rad50-Nbs1 complex is sufficient to restore the DNA double-strand break response in cells lacking RecQL4 helicase activity. (nih.gov)
  • Expression of MRE11 complex (MRE11, RAD50, NBS1) and hRap1 and its relation with telomere regulation, telomerase activity in human gastric carcinomas. (nature.com)
  • 2006). The trimeric Mre11-Rad50-Nbs1 (MRN) complex binds to DSB and forms foci rapidly and independently of ATM following DSB (Mirzoeva et al. (nih.gov)
  • The Mre11-Rad50-Nbs1 complex is represented as MRN (B2). (nih.gov)
  • Each Nbs1(also referred to as Nibrin) molecule recruits a dimer of Mre11 & Rad50 to the DSB focus with the CTD of Nbs1 bound to Mre11 (Nakanishi et al. (nih.gov)
  • 2001). Nbs1 also translocates the Mre11-Rad50 complex to the nucleus (Desai-Mehta et al. (nih.gov)
  • Inhibition of POLθ DNA polymerase activity leaves fork gaps unprotected, enabling their cleavage by the MRE11-NBS1-CtIP endonuclease, which produces broken forks with asymmetric single-ended DSBs, hampering BRCA2-defective cell survival. (unimi.it)
  • Based on these results, we speculate that the Mre11 nuclease complex may mediate the nucleolytic digestion of the 5′ strand at secondary structures formed upon DNA strand separation. (nebraska.edu)
  • Mre11 has a 3′ to 5′ exonuclease activity that results in the release of mononucleotides. (nebraska.edu)
  • MRE11, which contains a poly( ADP )-ribose binding motif and associates with PARP1, possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. (bvsalud.org)
  • Mutations in the MRE11 gene are associated with ATAXIA-TELANGIECTASIA-like disorder 1. (bvsalud.org)
  • Examining oocytes from mice, and from women 24 to 41 years old, the researchers found that the activity of four DNA repair genes (BRCA1, MRE11, Rad51 and ATM) declined with age. (nih.gov)
  • DYNLL1 binds to MRE11 to limit DNA end resection in BRCA1-deficient cells. (broadinstitute.org)
  • In vitro, DYNLL1 binds directly to MRE11 to limit its end-resection activity. (broadinstitute.org)
  • p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation. (ox.ac.uk)
  • The inhibition of p97 function results in excessive 5'-DNA end resection mediated by MRE11 that leads to defective DNA repair and radiosensitivity. (ox.ac.uk)
  • A Rare Case of Ataxia-Telangiectasia-Like Disorder With MRE11 Mutation. (cdc.gov)
  • Effect of MRE11 overexpression and knockdown on cell proliferation, invasion, and radioresistance was assessed in vitro using breast cancer cell lines (MCF-7 and MDA-MB-231). (medscape.com)
  • Effect of MRE11 overexpression on tumor growth was assessed in an orthotopic xenograft model (n = 8 mice per group). (medscape.com)
  • U mutanata mre11-2 tretiranih bleomicinom rezultati RT-PCR-a pokazali su smanjenu transkripciju gena CYCB1;1, biljega faze G2/M staničnog ciklusa, te gena ključnih za popravak DNA (BRCA1, RAD51, PARP1), za razliku od povećane ekspresije tih gena kod biljaka divljeg tipa. (unist.hr)
  • Ekspresija gena staničnog odgovora u mre11-2 mutantnoj liniji Arabidopsis thaliana (L.) nakon indukcije dvolančanih lomova DNA Bleomicinom', Diplomski rad, Sveučilište u Splitu, Prirodoslovno-matematički fakultet, citirano: 10.12.2023. (unist.hr)
  • In this study, we analyze the effects of Mre11 loss on the mechanism of vertebrate NHEJ by using a chromatinized plasmid double-strand break (DSB) repair assay in cell-free extracts from Xenopus laevis. (mdc-berlin.de)
  • We investigated the role of MRE11 in breast cancer in both clinical and in vitro settings. (medscape.com)
  • Mre11-depleted extracts are able to support efficient NHEJ repair of DSBs regardless of the end structure. (mdc-berlin.de)
  • 16. Mre11 regulates CtIP-dependent double-strand break repair by interaction with CDK2. (nih.gov)
  • Methods We examined MRE11 expression in tumor tissues from invasive ductal carcinoma breast cancer patients (n = 254) by immunohistochemistry, and associations with clinicopathological characteristics and overall survival were assessed using Cox proportional hazards regression models and Kaplan-Meier survival curves. (medscape.com)
  • Results Of the 254 tissue samples, 69.3% and 30.7% showed high and low MRE11 expression, respectively. (medscape.com)
  • Conclusion High MRE11 expression was associated with a more malignant behavior in breast cancer. (medscape.com)
  • Storage Conditions: MRE11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. (hatinhibitor.com)
  • Immunogen: MRE11 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus human MRE11.The immunogen is located within amino acids 40 - 90 of MRE11. (hatinhibitor.com)
  • Analysis of RPA and Mre11 in fractionated lysates (cytoplasmic/nucleoplasmic, chromatin-bound, and nuclear matrix fractions) showed increased hyperphosphorylated-RPA and phosphorylated-Mre11 in the chromatin-bound fractions. (nih.gov)
  • Analysis of MRE11 and Mortality Among Adults With Muscle-Invasive Bladder Cancer Managed With Trimodality Therapy. (moffitt.org)
  • Mre11 depletion does not alter the kinetics of end joining or the type and frequency of junctions found in repaired products. (mdc-berlin.de)
  • Mechanistically, this is mediated via increased MRE11 nuclease accumulation. (ox.ac.uk)