Paramount among these is the robustness of early embryos and their amenability to microinjection and microsurgery. (wikipedia.org)
The large size of embryos and amenability to microinjection also makes them extremely well suited to microarray approaches. (wikipedia.org)
Here we present a method for transgenesis in using microinjection of DNA into single-cell stage embryos and demonstrate its robustness by generating multiple transgenic tissue-specific reporter lines. (careersfromscience.org)
First, we tested how the egg handling and microinjection process itself impacts survival of the embryos (Supplementary Table?1). (careersfromscience.org)
Next, we injected in vitro synthesized mRNA encoding green fluorescent proteins (GFP) and noticed its expression in every effectively injected embryos (embryos are amenable to microinjection. (careersfromscience.org)
Xenopus embryos are large and easily manipulated, so that the function of various macromolecules, such as RNA and protein, can be assayed by microinjection into living embryos. (xenbase.org)
Embryo2
Rothbächer, U. (2016): Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis. (uibk.ac.at)
Methods: Knockdown or even overexpression of BDNF was brought on within hippocampus or VTA involving rats through microinjection of specific lentiviral vectors. (tgf-betasignal.com)
We therefore performed cortical unilateral microinjections of BDNF in awake rats and measured SWA during the subsequent sleep period. (ox.ac.uk)
Approaches1
Approaches covered will include microinjection and molecular manipulations such as CRISPR/Cas9 knockouts, antisense morpholino-based depletions, transgenics, and mRNA overexpression. (cshl.edu)
Process1
In the fertilization process, classical or microinjection methods are preferred depending on the condition of the reproductive cells. (mommemos.com)
Genetic2
Twenty five years ago Genetic Engineering was a scene from an unrealistic invention on a scene from a science fiction movie. (bartleby.com)
The most reliable method has been proven both effective and safe, but it requires genetic manipulation of in vitro roses by microinjection techniques using DNA fluorotypes (genes that determine flower color) taken from pansies, carnations and other sources of blue pigment. (dudeasks.com)
Methods1
We reasoned that microinjection methods used in additional model organisms, such as eggs (Fig.?1d, Supplementary Movie?1). (careersfromscience.org)
Image2
Secondary-click and "save image/movie" or "save page as" to download enlargement. (xenbase.org)
Next, create a microinjection image and a vertical black matte mat sha. (bothdrop.com)
Download3
These images and movies are available for display and download. (xenbase.org)
David RodgersCarlson N, Rogers S, Rechsteiner M. Microinjection of download appearances in: costumes in wood playwright in HeLa groups was to Part. (jollyrodgers.net)
These are ' La download american folk songs y la santeria en Nueva York y la Habana '( Culturas Encontradas: Cuba y los Estados Unidos, Introduction. (jollyrodgers.net)
Xenopus4
Xenbase provides a large database of images illustrating the full genome, movies detailing embryogenesis, and multiple online tools useful for designing and conducting experiments using Xenopus. (wikipedia.org)
A novel method for microinjection into Xenopus eggs and embryos supported in methylcellulose solution. (xenbase.org)
Possible projects in my lab would include the functional characterization of these new genes using embryological and cell biological techniques in Xenopus, such as microinjection, antisense depletion of maternal mRNAs, gene expression analysis and studies of morphogenesis. (xenbase.org)
a Microinjection strategy for a-f, and representative phenotypes of Xenopus laevis tadpoles (St. 32) injected with the indicated mRNAs radially at 4-cell stage. (xenbase.org)
Sustained depletion1
Here the need for genetic manipulations is replaced by physical microinjection of purified antibodies, which requires specialized equipment and sustained depletion requires repeated injections. (nature.com)
Quicktime1
We recommend VLC media player for viewing Quicktime movies on Windows system. (cmu.edu)
Multiple1
A microinjection visualizes multiple arterial feeds to a part of the fistulous arterial channel (pink), and portions of the sigmoid sinus are visible. (neuroangio.org)
Live1
Cell labeling was achieved by a painstaking process involving protein purification, fluorescence conjugation, and microinjection into live cells, before the convenient GFP technology became widely used in the 1990's. (cmu.edu)
Early1
was delivered to zebrafish through caudal vein microinjection during distinct periods in early neurodevelopment. (nih.gov)
Treatment1
Consisting of a cutting-edge, ultra-complete injection of powerful ingredients able to inhibit and notably soften wrinkles and expression lines, combined with advanced facial massage techniques, this non-invasive treatment achieves a powerful Botox effect without microinjections. (formacaulovers.com)