As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis. (cellsignal.com)
Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.com)
Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. (cellsignal.com)
Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.com)
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
Western blot analysis of HepG2 cell lysate using P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/4844R). (neobiotechnologies.com)
IL2RA polyclonal antibody CSB-PA011649LA01HU was produced in rabbit immunized by using the Recombinant Human Interleukin-2 receptor subunit alpha protein (22-185AA) as the immunogen. (cusabio.com)
Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is CDK6 (E3E3Q) Rabbit mAb. (cellsignal.com)
Anti-rabbit IgG1
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody. (cellsignal.com)
CDK4 and CDK62
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. (neobiotechnologies.com)
miR-34a inhibits CDK2 CDK4 and CDK6 as well as E2F a transcription factor that after release from the retinoblastoma tumor suppressor Rb initiates S-phase entry. (careersfromscience.org)
Polyclonal antibody1
This Rabbit anti-Homo sapiens (Human) IL2RA Polyclonal antibody was tested in the ELISA, WB, IF and IHC. (cusabio.com)
Rabbit6
Western blot analysis of extracts from various cell lines using CDK6 (E3E3Q) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
using CDK6 (E3E3Q) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
Western blot analysis was performed using CDK6 (E3E3Q) Rabbit mAb. (cellsignal.com)
Immunohistochemical analysis of paraffin-embedded normal human colon (two fields of view) using CDK6 (E3E3Q) Rabbit mAb (left) or a CDK6 Rabbit mAb (right). (cellsignal.com)
Formalin-fixed, paraffin-embedded human cervix stained with P16INK4aRecombinant Rabbit Monoclonal Antibody (CDKN2A/4844R). (neobiotechnologies.com)
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. (affbiotech.cn)
Reacts1
It reacts with the IL2RA proteins of human or mouse-origin and may be used to detect the endogenous levels of IL2RA protein. (cusabio.com)
Reactivity1
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Activin A Receptor Type II A (ACVR2A) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (lotuskringpoeldijk.nl)
Protein4
Low expression of CDK6 protein in ZR-75-1, Neuro-2a, and INS-1 cells is consistent with the predicted expression pattern. (cellsignal.com)
Immunoprecipitation of CDK6 protein from Jurkat cell extracts. (cellsignal.com)
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. (abcam.cn)
The non-conjugated IgG got purified by protein G and reached up to 95% in purity. (cusabio.com)
Inhibitor2
Based on a database search, we identified abemaciclib, an FDA-approved Cdk4/Cdk6 inhibitor used for the treatment of metastatic breast cancer, as potent inhibitor of HIPK2, HIPK3, and DYRK1A. (nature.com)
It is a specific inhibitor of cdk4/cdk6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. (neobiotechnologies.com)
Endogenous levels1
Hsp90 beta Antibody detects endogenous levels of total Hsp90 beta. (affbiotech.cn)
Sensitivity1
At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
GAPDH1
Description: Mouse monoclonal to GAPDH (Human Specific). (sebov-detection.com)
Lysates1
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Activin A Receptor Type II A (ACVR2A) in tissue homogenates, cell lysates and other biological fluids. (lotuskringpoeldijk.nl)
Vitro2
In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. (cellsignal.com)
Remarkably, DYRK1A is inhibited by abemaciclib to the same extent as Cdk4/Cdk6 in vitro, raising the question of whether targeting of DYRK1A contributes to the transcriptional inhibition and therapeutic activity of abemaciclib. (nature.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kinase 1 Delta (CSNK1d) in Tissue homogenates and other biological fluids. (1elisakits.com)
Precursor1
miRNAs are a recently discovered class of small evolutionary highly conserved non-coding RNAs of 18-25 nucleotides length which are generated by sequential processing of primary PD173955 and Mouse monoclonal to alpha Actin precursor miRNA by the ribonucleases Drosha and Dicer1. (careersfromscience.org)
Specificity1
The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. (cellsignal.com)
Primary1
Anti-ZKSCAN5 (SAB4501021), anti-VEGFC (ab83905), and anti-LYVE1 (ab10278) primary antibodies were used at 1:100, 1:100, and 1:50 dilutions, respectively. (bms-911543.com)
IHC-P analysis of human tonsil tissue using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
Human Antibody Laboratories manufactures the names of specialty perscription human anti bodies reagents distributed by Genprice. (sebov-detection.com)
Serum1
The mice had been pre-treated with anti-PRV swine serum and challenged with YS-81 predicated on an operation reported by Osorio and Rock and roll [13]. (colinsbraincancer.com)
Specific1
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
Host1
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
Product3
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you can trust. (cellsignal.com)
The IL2RA Antibody (Product code: CSB-PA011649LA01HU) is Non-conjugated. (cusabio.com)
Support1
in situations where an antibody will be used to support large, long-term studies, this can be an especially critical factor. (cellsignal.com)
Research1
This antibody is available for research use only and is not approved for use in diagnosis. (neobiotechnologies.com)
Analysis1
WB analysis of HeLa cell lysate using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
Time1
The tumour size was calculated, and the mice were euthanised at the indicated time. (bms-911543.com)
Region1
We determined the crystal structures of HIPK3 and DYRK1A bound to abemaciclib, showing a similar binding mode to the hinge region of the kinase as observed for Cdk6. (nature.com)
Cells3
For tumour estimation, nude mice were inoculated subcutaneously with 1 107 ZR75-1 cells with different constructs on the right side. (bms-911543.com)
BALB/c mice were injected with 1 106 MDA-MB-231 cells labelled with luciferase carrying the indicated constructs into the lateral tail vein. (bms-911543.com)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
Stress1
We've also founded a PRV latent illness model in mice using the crazy PRV YS-81 stress [20]. (colinsbraincancer.com)