Mouse hela cells. Medical search. Frequent questions

WB : NIH/3T3 (mouse embryonic fibroblast cell line) nuclear lysate (triton enriched), PC-12 (rat adrenal gland pheochromocytoma cell) nuclear lysate (triton enriched). (abcam.com)
Detection:22225-1-AP 1:600) with HeLa cells lysate 1600ug. (ptglab.com)
No. ADI-SPP-848), Lane 3: HL-60 Cell Lysate, Lane 4: Hep G2 Cell Lysate, Lane 5: 3T3 Cell Lysate, Heat Shocked (Prod. (enzolifesciences.com)
No. ADI-LYC-3T101), Lane 6: HeLa Cell Lysate, Heat Shocked (Prod. (enzolifesciences.com)
Detection:10837-1-AP 1:800) with mouse brain tissue lysate 8000ug. (ptglab.com)
Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB100-58804] - Detection of Human and Mouse UBE1 on HeLa whole cell lysate using NB100-58804. (novusbio.com)
Mouse testis tissue lysate. (abcam.cn)

Purified mouse monoclonal antibody. (sigmaaldrich.com)
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. (abcam.com)
The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads. (abcam.com)
Each modified S protein was displayed on the surface of rMVA-infected human cells and was recognized by anti-RBD antibody and by soluble hACE2 receptor. (nih.gov)
HL-60 cells were subjected to SDS PAGE followed by western blot with 22225-1-AP (MPO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. (ptglab.com)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 40x lens. (ptglab.com)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 10x lens. (ptglab.com)
12. Downregulation of membrane complement inhibitors CD55 and CD59 by siRNA sensitises uterine serous carcinoma overexpressing Her2/neu to complement and antibody-dependent cell cytotoxicity in vitro: implications for trastuzumab-based immunotherapy. (nih.gov)
Two of these three antibodies (anti-citrullinated protein antibody and anti-keratin antibody) are found in the perinuclear region of the cell, whereas anti-RA33 antibody is found in the nucleolar region of the cell. (medscape.com)
Anti-RA33 antibody was discovered by Hassfeld et al in 1989 and was found to bind a specific nuclear protein in HeLa cells. (medscape.com)
Further studies done in arthritis mouse models established that this antibody is against a spliceosome, which is a heterogeneous ribonucleoprotein complex (36 KDa) A2 protein (hnRNP A2). (medscape.com)
The cells were then stained with BD Phosflow™ R718 Mouse Anti-Human NF-κB p65 (pS529) antibody (Cat. (bdbiosciences.com)
Cells were incubated with Anti-Prohibiton antibody and labeled with Alexa Fluor 594 Tyramide SuperBoost Kit , Goat anti-Mouse IgG. (thermofisher.com)
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Immunocytochemical staining of phospho-hsp27 (Ser15) in HeLa cells with this antibody results in cytoplasmic staining. (thermofisher.com)
ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. (novusbio.com)
Description Western Blot analysis of Rat Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 2A4 (11116). (qedbio.com)
Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11116) at 1:5000 for 2 hours at RT. (qedbio.com)
Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. (qedbio.com)
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 2A4 (11116). (qedbio.com)
Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (11116) at 1:100 for 60 min at RT. (qedbio.com)
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. (qedbio.com)
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OPTN (10837-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. (ptglab.com)
HeLa cells were stained with Mouse Anti-Human Insulysin/IDE Monoclonal Antibody (Catalog # MAB2496, filled histogram) or isotype control antibody (Catalog # MAB002 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab') 2 Secondary Antibody (Catalog # F0101B ). (rndsystems.com)
Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal antibody and anti-CTNNB1 mouse monoclonal antibody. (abnova.com)
We will then outline routes for antibody and immune cell entry into the CNS, with a focus on the predominant pathways leading to BBB breakdown that allows entry of autoantibodies into the CNS. (frontiersin.org)
Anti-mouse IgG, HRP-linked Antibody ( #7076 ). (cellsignal.com)

Publications are still being populated for the years 2016, 2017, and 2018 and the number of publications using HeLa cells per year are lower as a result. (nih.gov)

Moreover, cervical cancer HeLa cells overexpressing miR-215 (HeLa-miR-215) were constructed and subcutaneously injected into the nude mice to examine the effect of miR-215 on tumor growth and metastasis in vivo. (nih.gov)
1. A study on screening and antitumor effect of CD55-specific ligand peptide in cervical cancer cells. (nih.gov)
2. Targeted Antitumor Mechanism of C-PC/CMC-CD55sp Nanospheres in HeLa Cervical Cancer Cells. (nih.gov)
3. The Antitumor Efficiency of Zinc Finger Nuclease Combined with Cisplatin and Trichostatin A in Cervical Cancer Cells. (nih.gov)
4. Human papillomavirus E6 protein enriches the CD55(+) population in cervical cancer cells, promoting radioresistance and cancer aggressiveness. (nih.gov)
5. Screening and identification of a specific peptide binding to cervical cancer cells from a phage-displayed peptide library. (nih.gov)
6. The Effects Of Sevoflurane On The Progression And Cisplatinum Sensitivity Of Cervical Cancer Cells. (nih.gov)
7. Anti-proliferative and pro-apoptotic effects of 3,3'-diindolylmethane in human cervical cancer cells. (nih.gov)
8. Verteporfin Promotes the Apoptosis and Inhibits the Proliferation, Migration, and Invasion of Cervical Cancer Cells by Downregulating SULT2B1 Expression. (nih.gov)
9. Carvacrol Exhibits Chemopreventive Potential against Cervical Cancer Cells via Caspase-Dependent Apoptosis and Abrogation of Cell Cycle Progression. (nih.gov)
11. Interaction of C20-substituted derivative of pregnenolone acetate with copper (II) leads to ROS generation, DNA cleavage and apoptosis in cervical cancer cells: Therapeutic potential of copper chelation for cancer treatment. (nih.gov)
13. The anticancer effect of extract of medicinal mushroom Sanghuangprous vaninii against human cervical cancer cell via endoplasmic reticulum stress-mitochondrial apoptotic pathway. (nih.gov)
15. Combination therapy Eve and Pac to induce apoptosis in cervical cancer cells by targeting PI3K/AKT/mTOR pathways. (nih.gov)
16. [Clinical significance of σ1 receptor over-expression in cervical cancer and the effect of its synthetic ligands on the growth of cervical cancer cells]. (nih.gov)
Diels induces the apoptosis of cervical cancer HeLa cells through an intrinsic apoptotic pathway. (nih.gov)
19. Sesamin inhibits cervical cancer cell proliferation by promoting p53/PTEN-mediated apoptosis. (nih.gov)
Tissue: Cervical cancer cell line (HeLa). (qedbio.com)
A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. (nih.gov)
HeLa cells (human cervical cancer cell lines) were stained with CD49a (clone TS2/7) PerCP/Cyanine 5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine 5.5 isotype control (open histogram). (biolegend.com)

Protein binding depends on the level of HS sulfation and epimerization which are cell specific and dynamically regulated. (nih.gov)
Recognizes the SQSTM1 protein HeLa cells. (sigmaaldrich.com)
ture sample had protein and glucose levels of 4.56 g/L and Since the beginning of the 21st century, an explosive 0.1 mmol/L, respectively, and a leukocyte count of 4,700 spread of CTX-M-type extended-spectrum -lactamases cells/L (54% polymorphonuclear cells). (cdc.gov)
Mass spectrometry-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of protein networks in cells, but involves laborious workflows that does not cover the phospho-proteome level. (nature.com)
Protein function is tightly controlled in cells through multiple mechanisms. (nature.com)
Proliferating fibroblasts and HeLa cells co-cultured in vitro reciprocally influence growth patterns, protein expression, chromatin features and cell survival. (iiar-anticancer.org)
Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. (horizondiscovery.com)
The in situ proximity ligation assay is a powerful technology capable of detecting single protein events such as protein protein interactions (e.g. protein dimerization) and modifications (e.g. protein phosphorylation) in tissue and cell samples prepared for microscopy. (abnova.com)
The phosphorylation of DNMT1 protein on Y399 in HeLa cell. (abnova.com)
Plasma protein binding is approximately 65% and granisetron distributes freely between plasma and red blood cells. (nih.gov)
Increased FKBP38 protein levels and decreased PHD2 protein levels were found in PSEN1/2-deficient mouse embryonic fibroblasts and in the cortex of forebrain-specific PSEN1/2 conditional double knock-out mice. (jneurosci.org)
This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. (nih.gov)
The misprocessed protein is trapped intracellularly and associates more with chaperone Hsp70.This misfolded protein could be rescued to cell surface by growing at lower temperature (27°C), treatment with substrates (cyclosporine A), modulators (tariquidar) or small corrector molecules for 4-18 hrs. (nih.gov)
It was observed that mutation D800A when expressed in HeLa cells also resulted in misprocessing of this protein. (nih.gov)
Recently, we showed evidence that forces on the cell membrane can be transmitted directly into the nucleus, inducing structural changes of protein complexes in Cajal bodies. (researchsquare.com)

Furthermore, the stability of Nrf2 was dramatically decreased in IQGAP1-deficient mouse embryonic fibroblast (MEF) cells. (nih.gov)
Double minutes in fibroblast-like cells isolated from human tumors. (iiar-anticancer.org)
Interactions between human lymphoblastoid cells and human fibroblast feeder layers in vitro. (iiar-anticancer.org)
The Rhipicephalus rickettsia grew well in monolayers of chicken embryo fibroblast, Vero, mouse L, and HeLa cells. (duke.edu)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)

Flow Cyt (intra): HeLa cells. (caslab.com)

Tumor volume in nude mice injected with HeLa-miR-215 cells was significantly larger than that in mice injected with control HeLa cells. (nih.gov)
From NCBI Gene: This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. (nih.gov)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. (novusbio.com)
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. (novusbio.com)

IHC-Fr: Mouse testis tissue. (caslab.com)

Applied to wild-type and N-deacetylase/N-sulfotransferase-deficient fibroblasts as well as to epithelial cells differentiation, it also gives insights into the functional relationships existing between HS biosynthetic enzymes. (nih.gov)
Fibroblasts against cancer cells in vitro. (iiar-anticancer.org)
Teaming Up for Trouble: Cancer Cells, Transforming Growth Factor-β1 Signaling and the Epigenetic Corruption of Stromal Naïve Fibroblasts. (iiar-anticancer.org)

Direct interactions between IQGAP1 and Nrf2 proteins were verified using in vitro glutathione S-transferase (GST) pull-down, transcription/translation assays, and in vivo utilizing Nrf2 overexpressing cells. (nih.gov)
We benchmark the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in vitro in HeLa cells and in vivo in mouse tissues. (nature.com)
in transient transfection assays, increased expression of adipogenic genes, promoted adipogenic differentiation and increased lipid accumulation in mouse and human MSCs, in vitro . (nih.gov)
Cell-cell interactions, stromal cells, drug resistance, in vitro evaluation of anticancer agents, mechanisms of invasion and metastasis. (iiar-anticancer.org)
Interactions between human fibroblastsand HeLa cells in vitro. (iiar-anticancer.org)
In an effort to further understanding of the effects of radiofrequency (RF) radiation on somatic cell mitotic activity and germ cell function, cell suspensions were exposed to 27 megahertz (MHz) or 2,450MHz radiation under isothermal conditions in-vitro. (cdc.gov)

We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells. (amsbio.com)
Last, we observed TREM1+ cells in clinical brain biopsy samples from two treatment-naïve patients with MS but not in healthy control brain tissue. (stanford.edu)
mouse and rat brain tissue lysates. (caslab.com)
Cytolytic Effects of Mumps Virus in Tissue Cultures of Epithelial Cells. (nih.gov)
Leveraging on Abnova's manufacturing capability and growing collection of 50,000+ antibodies, hundreds of target specific and validated assays are being developed in cells and tissue samples. (abnova.com)
Investigations dealing with inherently low-contrast specimens, such as unstained bacteria, thin tissue slices, and adherent live cells, rely on specialized contrast-enhancing techniques to assist with imaging these virtually transparent samples. (fsu.edu)
The thin tissue section in Figure 1(a) reveals a human basal cell carcinoma stained with eosin and hematoxylin to generate color contrast in brightfield imaging mode. (fsu.edu)
Figure 1(b) shows living HeLa cells in a plastic tissue culture vessel imaged with phase contrast. (fsu.edu)
A fresh tissue section of mouse heart muscle bathed in aqueous saline solution is displayed in the Figure 1(d) panel, where contrast is generated using Hoffman modulation (or ZEISS VAREL) contrast, an oblique illumination technique. (fsu.edu)

A conditional knockout mouse line, called Rnaseh2btm1a(EUCOMM)Wtsi was generated as part of the International Knockout Mouse Consortium program - a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. (wikipedia.org)
Rnaseh2b gene knockout in mice leads to early embryonic lethality, hence genetically engineered mice with a premature stop codon in exon 7 Rnaseh2b was created. (wikipedia.org)
Staurosporine (STS) elicits long-lasting Ca 2+ elevations in wild-type but not in IP 3 R-knockout HeLa cells, sensitizing the former to STS treatment. (nature.com)
Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. (ubigene.us)

Deficient RNASEH2 activity in mice causes white matter defects, neuroinflammation and atrophy of the cerebellum, conditions that mirror Aicardi-Goutieres syndrome in humans. (wikipedia.org)

We evaluated the effects of DBT on nuclear receptor activation and adipogenic potential using human and mouse multipotent mesenchymal stromal stem cells (MSCs). (nih.gov)
Nonrandom association of acrocentric chromosomes in human epithelial cells. (iiar-anticancer.org)
Cultured cells from the human HeLa S3 (Cervical adenocarcinoma, ATCC CCL 2.2) cell line were starved overnight in Dulbecco's Minimal Eagle's Medium. (bdbiosciences.com)
ICC/IF: HeLa, human adenocarcinoma, human schlemms canal endothelium and wild-type HAP1 cells. (caslab.com)
Cell types included resting human peripheral lymphocytes, LN71 or RT2 glioma cells, Chinese-hamster-ovary cells, HeLa cells, and mouse spermatozoa. (cdc.gov)
First, we discovered that in human HCT116 colon cancer cells, knock-out (KO) of TOP3B had similar effects on mRNA turnover and translation as did TDRD3-KO, while FMRP-KO resulted in rather distinct effects, indicating that TOP3B had stronger coordination with TDRD3 than FMRP in mRNA regulation. (nih.gov)
COS 7 cell expressing Organelle LightsTM Golgi-GFP (Invitrogen)consisting of the human Golgi-resident enzyme N- acetylgalactosaminyltransferase 2. (cellimagelibrary.org)
Organization of Golgi and endoplasmic reticulum (ER) in a Human HeLa cell expressing Sialyl Transferase-EGFP (green) and Invariant Chain33-mRFP (red). (cellimagelibrary.org)
Formerly associated with the Naval Biological Laboratories in Oakland, California, and the development of a cell bank which was initially contracted with Dr. Stewart Madin, which evolved into a facility for both animal and human normal and cancer cell lines. (nih.gov)
We dealt with a variety of other domestic animals, at first dogs and cats, and then animals that were of importance in the matter of human nutrition, cells from cattle, cells from goats, cells from cows, cells from sheep. (nih.gov)
We never actually obtained these, but it was always a relationship to the human, to the animal, to the domestic, to cells that might relate. (nih.gov)
And it turned out that a number of instances were delineated where cell lines had gotten mixed up and there was a lot of contamination, where dog and cat and calf and other existing cell lines were mixed up and thought to be human, and vice versa, and that there was PPLO, or Mycoplasma , as we now call it, rampant as infections in these cultures. (nih.gov)
The homology model of human P-gp based on crystal structure of mouse P-gp shows SNPs D800N, V801M and D1088N are located spatially close to Asp805 and all these residues together result in a tight cluster. (nih.gov)
Species Reactivity: Human, does not react with mouse and rat. (scytek.com)

Ribonucleotide incorporation occurs in metazoans and these lesions are harmful to mammalian cells, and their removal is required for mouse embryonic development. (wikipedia.org)
1.000.000 sites per cell, making it the most common endogenous base lesion in the mammalian genome. (wikipedia.org)
Ribonucleotides can be highly deleterious to the mammalian cell, causing genome instability, and that the RNaseH2 is a critical enzyme for ensuring integrity of genomic DNA. (wikipedia.org)
Fractionation of nucleic acids from mammalian cells in culture by polylysine-Kieselguhr column chromatography. (iiar-anticancer.org)
Typically, mammalian cells are sensitive to Zeocin ® concentrations of 50-400 µg/ml, and bacteria to 25 µg/ml. (invivogen.com)
1997. Overexpression of DNA polymerase beta sensitizes mammalian cells to 2',3' deoxycytidine and 3'-azido-3'-deoxythymidine. (invivogen.com)
High-efficiency transformation of mammalian cells by plasmid DNA. (nih.gov)
Studies were also conducted on liposome permeability, and a mathematical model was used to calculate radiation energy absorption in mammalian cell models. (cdc.gov)

Biol Cell 59(1):69-77, 1987. (iiar-anticancer.org)
Delinassios JG, Kottaridis SD and Garas J. Exp Cell Biol 51(4):201-9, 1983. (iiar-anticancer.org)
Biol Cell 50(1):9-16, 1984. (iiar-anticancer.org)
Exp Cell Biol 49(4):177-84, 1981. (iiar-anticancer.org)
J Cell Biol (2005) 168 (1): 41-54. (rupress.org)

Since IQGAP1 signaling could be mediated by calcium, treating the cells with calcium showed the translocation of IQGAP1/Nrf2 complex into the nucleus, suggesting that IQGAP1 may play a critical role in Nrf2 stability. (nih.gov)
For HeLa cell nucleus volume of 690 um^3 see BNID 104716 . (harvard.edu)
Nucleus was labeled with NucBlue Fixed Cell ReadyProbes Reagent . (thermofisher.com)
Bovine pulmonary artery endothelial cell labeled with probes to visualize mitochondria, peroxisomes, and the nucleus. (thermofisher.com)
Stem cells differentiate in respond to different surface topology 11 , substrate rigidity 12 , and applied stress 13 , a clear indication of gene expression change within the nucleus. (researchsquare.com)
Because gene expression in a live cell involves many complicated processes in the cytoplasm and the nucleus and takes time, it is impossible, at the present time, to attribute any changes in gene expression to direct effects of the applied force at the cell surface, without involving intermediate biochemical signaling cascades. (researchsquare.com)

In a mouse model of DMD, serum-stabilized Pip2a or Pip2b peptides conjugated to a 20-mer PNA (PNADMD) targeting the exon 23 mutation in the dystrophin gene showed strong exon-skipping activity in differentiated mdx mouse myotubes in culture in the absence of an added transfection agent at concentrations where naked PNADMD was inactive. (ox.ac.uk)
Esterase gene expression in Chinese hamster and mouse lymphoma hybrids isolated under nonselective pressure. (iiar-anticancer.org)
Therefore, Zeocin® is a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells). (invivogen.com)
At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide. (ubigene.us)
For gene-editing mice. (ubigene.us)
When the IDE gene was specifically disrupted in mice, IDE -/- animals developed hyperinsulinemia and glucose intolerance, characteristics of DM2 (5). (rndsystems.com)
This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. (novusbio.com)
However, little is known about the mechanism by which individual cells sense the mechanical forces and convert them in to biochemical signals within the cell and influence the gene expression, a process known as mechanotransduction. (researchsquare.com)

HeLa or HepG2 cells. (biotium.com)

Perinatal exposure to low doses of DBT led to increased fat storage, decreased glucose tolerance, and increased circulating leptin levels in male, but not female, mice. (nih.gov)
In vivo exposure to biologically relevant doses of DBT during perinatal development led to increased fat storage, elevated leptin levels in plasma, and glucose intolerance in mice. (nih.gov)

Finally, we demonstrate that endogenous Bcl-xL also suppress IP 3 R activity in MDA-MB-231 breast cancer cells, whereby Bcl-xL knockdown augmented IP 3 R-mediated Ca 2+ release and increased the sensitivity towards STS, without altering the ER Ca 2+ content. (nature.com)

We show that Pip-PNA705 conjugates are internalized in HeLa cells by an energy-dependent mechanism and that the predominant pathway of cell uptake of biologically active conjugate seems to be via clathrin-dependent endocytosis. (ox.ac.uk)
18. Mitofusin-2 Triggers Cervical Carcinoma Cell Hela Apoptosis via Mitochondrial Pathway in Mouse Model. (nih.gov)
Therefore, we investigated the role of PSEN1/2 in the oxygen sensing pathway using a variety of genetically modified cell and mouse lines. (jneurosci.org)

[ 83 , 84 ] PDT has been predominantly used for cancer treatment by causing localized damage to tumor cells and vasculatures. (medscape.com)
Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications. (amsbio.com)
Antibodies directed against neuronal antigens can arise in the periphery when target proteins are expressed ectopically on tumor cells. (frontiersin.org)
Cells integrate both chemical and mechanical cues to regulate biological processes as diverse as differentiation, vascular development, tumor growth and malignancy 1-5 . (researchsquare.com)

18F]DASA-10 demonstrated excellent cellular uptake and retention with values significantly higher than [18F]DASA-23 in all cell lines and timepoints investigated. (stanford.edu)
18F]DASA-10 showed a 73 % and 65 % reduced uptake respectively in DU145 and HeLa cells treated with PKM2 siRNA as compared to control siRNA treated cells. (stanford.edu)
Using a stable OEA analog, KDS-5104, we found that OEA-PPARα signaling promotes TFEB lysosomal activity independent of mTORC1 and upregulates cell-surface receptor CD36, leading to enhanced microglial Aβ uptake and clearance. (nih.gov)

Improved cell-penetrating peptide-PNA conjugates for splicing redirection in HeLa cells and exon skipping in mdx mouse muscle. (ox.ac.uk)

This Anti-SQSTM1 Mouse mAb (2C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of SQSTM1. (sigmaaldrich.com)

Cell volumes are from Lynch and Marinov (2015) , in some cases supplemented with additional references from the literature. (elifesciences.org)

Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. (nature.com)
To trace these proteins, scientists in her lab metabolically label cells with precursor sugars tagged with clickable handles that can be modified with imaging probes. (acs.org)
Here we describe peroxisome stains using organelle-specific fluorescent fusion proteins in live cells or antibodies to peroxisome membrane proteins in fixed cells. (thermofisher.com)
CellLight fluorescent fusion proteins can be used as peroxisome labels in live cells and to follow the dynamics of intracellular behavior. (thermofisher.com)
Introducing CellLight fluorescent proteins involves a simple transfection step using the BacMam technology, and they work like cell stains with minimal toxicity or chemical disruption. (thermofisher.com)
These peroxisome fusion proteins are compatible with other fluorescent probes for multiplex analysis in live cells (Figures 1 and 2) , or after formaldehyde fixation for colocalization studies. (thermofisher.com)
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. (thermofisher.com)

Intramuscular injection of mice with the rMVAs induced S-binding and pseudovirus-neutralizing antibodies. (nih.gov)
[ 7 , 8 ] Past clinical evidence has demonstrated the production of several autoantibodies, including RF and other anti-collagen antibodies, in synovial plasma cells in response to RA pathogenesis. (medscape.com)
We then discuss the routes that antibodies and immune cells employ to enter the CNS and their implications for AE. (frontiersin.org)

The effect of diphtheria toxin on the metabolism of HeLa cells. (nih.gov)

Maul GG, Deaven L. Quantitative determination of nuclear pore complexes in cycling cells with differing DNA content. (harvard.edu)
Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. (nih.gov)
Simple brightfield imaging, with the microscope properly adjusted for Köhler illumination, provides a limited degree of information about the cell outline, nuclear position, and the location of larger vesicles in unstained specimens. (fsu.edu)
Although there have been ample reports showing that cytoplasmic enzymes can be directly activated by a local stress on the cell surface via integrins, there has been no evidence that mechanical forces can directly alter nuclear functions without intermediate biochemical cascades. (researchsquare.com)

Western blot analysis of extracts from various tissues using DJ-1 (E9T6J) Mouse mAb. (cellsignal.com)
Several of these techniques are limited by light originating in regions removed from the focal plane when imaging thicker plant and animal tissues, while polarized light requires birefringence (usually not present to a significant degree in animal cells) to generate contrast. (fsu.edu)

Folic acid was further conjugated for specific targeting to the colon cancer cells. (medscape.com)

We have used a HeLa pLuc705 cell splicing redirection assay to develop a series of PNA internalization peptides (Pip) conjugated to an 18-mer PNA705 model oligonucleotide with higher activity compared to a PNA705 conjugate with a leading cell-penetrating peptide being developed for therapeutic use, (R-Ahx-R)(4). (ox.ac.uk)

Cells were fixed and permeabilized with Image-iT Fixation/Permeabilization Kit . (thermofisher.com)

Establishment and characterization of a B-cell line from a patient with acute lymphoblastic leukemia. (iiar-anticancer.org)
Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)

[ 81 ] The mechanism of PDT consists of three stages: excitation of photosensitizers, generation of ROS and cell death. (medscape.com)

1975). The compound has been found to accumulate in the cauda epididymis of rats and to a lesser extent in that of mice, as observed by autoradiography (Crabo & Appelgren, 1972). (inchem.org)

These were the Cell Culture Characterization Program, the Cell Culture Characterization Laboratory (CCCL). (nih.gov)

This in vivo evidence is consistent with the hypotheses that IDE is important for the degradation of insulin in cells and for the clearance of A beta peptide in the brain. (rndsystems.com)

We then confirmed the specificity of [18F]DASA-10 by transiently modulating the expression of PKM2 in DU145 and HeLa cells. (stanford.edu)

A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. (nih.gov)
Detection of Insulysin/IDE in HeLa cells by Flow Cytometry. (rndsystems.com)

Peroxisomes are single membrane-bound vesicles found in most eukaryotic cells. (thermofisher.com)

The fluorescence histograms showing NF-κB p65 (pS529) expression were derived from gated events with the forward and side light-scatter characteristics of intact HeLa S3 cells. (bdbiosciences.com)

c ) Relationship between the total (inner + outer) surface area of mitochondria and cell volume for all species with available data. (elifesciences.org)

Anatomy9

Organisms6

Chemicals and Drugs33

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes31

Information Science3

Health Care1

Lysate7

Antibody27

Publications using HeLa1

Cervical cancer19

Protein15

Fibroblast4

Monoclonal1

Flow Cyt1

Nude mice2

Carcinoma cell line2

Testis tissue1

Fibroblasts3

Vitro6

Tissue9

Knockout4

Humans1

Human14

Mammalian8

Biol5

Nucleus6

Gene8

HepG21

Glucose2

Knockdown1

Pathway3

Tumor4

Uptake3

Exon1

ELISA1

References1

Proteins7

Antibodies3

Metabolism1

Nuclear4

Tissues2

Colon1

Assay1

Fixation1

Line2

Mechanism1

19751

Characterization1

Vivo1

Specificity1

Detection2

Eukaryotic1

Characteristics1

Mitochondria1