• Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. (rndsystems.com)
  • IHC-P: Human uterus, human smooth muscle and mouse smooth muscle tissues. (abcam.com)
  • Mouse lung and liver tissues. (abcam.cn)
  • While most of the decellularization protocols were originally developed for tissues, they have also more recently been adapted for CDMs from cultured cells. (researchsquare.com)
  • Eplin-α mRNA is detected in various tissues and cell lines, but strikingly absent or downregulated in cancer cells [ 2 ]. (biomedcentral.com)
  • Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. (bvsalud.org)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • WB: A431, Jurkat, Daudi and HeLa cell lysates. (abcam.com)
  • WB: Rat kidney and liver tissue lysate, A431, U-2 OS, HepG2 whole cell lysates. (abcam.cn)
  • The Comet Assay (also called Single Cell Gel Electrophoresis) is used to assess DNA damage and repair in individual cells. (neb.com)
  • Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
  • Using a tetrazolium-based assay, the dose required to reduce cell viability by 50% (ED 50 ) was found to be ∼1.8 μmol/L in FaDu (human hypopharyngeal squamous cancer) and ∼2.6 μmol/L in C666-1 (human undifferentiated nasopharyngeal cancer) cells. (aacrjournals.org)
  • Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. (angioproteomie.com)
  • Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. (wikipedia.org)
  • After killing the female mouse, the researcher should incise its stomach and then detach the embryo from the placenta in a biohazard hood. (wikipedia.org)
  • MEFs treated by mitomycin or gamma rays (such treatment makes MEF stop mitosis) are widely used as feeder in embryonic stem cell culture because they can mimic the microenvironment in embryo. (wikipedia.org)
  • In the present study, cytotoxic and apoptotic effects of melatonin were investigated on H-ras oncogene transformed rat embryo fibroblast 5RP7 cells. (tubitak.gov.tr)
  • This time lapse series illustrates the early division up to the four-cell stage of a wild-type C. elegans embryo by DIC microscopy. (ucsd.edu)
  • We showed inhibition of cell proliferation that was time- and dose-dependent in 5RP7 cells for 24 and 48 h. (tubitak.gov.tr)
  • Here we identify CSNK1E , the gene encoding casein kinase 1 epsilon (CK1ε) as required specifically for the proliferation of breast cancer cells with activated β-catenin and confirm its role as a positive regulator of β-catenin-driven transcription. (plos.org)
  • The Wnt/β-catenin pathway plays a critical role in embryonic development, maintenance of multipotent progenitor cell populations and proliferation of many tissue types [1] , [2] . (plos.org)
  • proteasome inhibition interferes with cell cycle progression, upregulates tumor suppressors such as p53, and diminishes activation of pro-proliferation pathways such as those controlled by NFB and extracellular signal-regulated kinases (ERKs) [3], [4]. (scienza-under-18.org)
  • Low levels of ROS can promote cell proliferation but high levels induce cell death [ 13 ]. (biomedcentral.com)
  • Fluorescence microscopy showed that the trimer and pentamer bound strongly to the cell surface, and within 5 minutes were prominently localized to actin fiber bundles. (biologists.com)
  • These links are used in cell motility as the actin cytoskeleton generates force pulling the integrin and attached matrix molecules towards the cell center. (biologists.com)
  • NIH 3T3 cell (mouse embryonic fibroblast line) stained for Actin (green) and DNA (blue). (ucsd.edu)
  • This protocol efficiently removes cytoskeletal proteins, including actin and beta-tubulin, and DNA from the cultures, which could interfere with the cell-matrix interactions of cells seeded on the decellularized matrices. (researchsquare.com)
  • In epithelial cells, Eplin is required for formation of the F-actin adhesion belt by binding to the E-cadherin-catenin complex through α-catenin [ 6 ]. (biomedcentral.com)
  • ICC: HeLa cells. (abcam.com)
  • It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
  • Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
  • Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)
  • HeLa human cervical carcinoma (ATCC CCL 2) and NIH 3T3 mouse fibroblasts (ATCC CRL 1658) are suggested as a positive controls for these applications. (bdbiosciences.com)
  • Flow Cyt (intra): HeLa cells. (abcam.com)
  • Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts. (jci.org)
  • These studies demonstrate differential regulation of Na/H antiporter activity and mRNA abundance in renal epithelial cells and fibroblasts in response to an acidotic environment. (jci.org)
  • Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
  • All isolated poha compounds (aerial parts and fruits) were evaluated for their anti-inflammatory activity with lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cells, and tumor necrosis factor alpha (TNF-α)-activated nuclear factor-kappa B (NF-κB) with transfected human embryonic kidney cells 293. (nih.gov)
  • This decellularization method has been used in a variety of contexts including primary human dermal and lung fibroblast cultures, cancer-associated fibroblast cultures from esophageal, ovarian, renal, pancreatic, prostatic and lung tumors, and murine mammary fibroblasts 10,12,13 . (researchsquare.com)
  • The MEF is a limited cell line. (wikipedia.org)
  • These cells developed into the commonly used cell line NIH 3T3. (wikipedia.org)
  • Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)
  • This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
  • We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
  • No PrPTSE or vCJD infections have been transmitted by transfusions of infectivity has been detected in any exposed cell line under nonleukoreduced erythrocyte concentrates and by a human- study so far. (cdc.gov)
  • Na/H antiporter activity was increased by 60% in a mouse renal cortical tubule cell line (MCT), and by 90% in an opossum kidney cell line (OKP) after 24 h of preincubation in acid (low [HCO3]) media. (jci.org)
  • The effect of phenethyl isothiocyanate (PEITC) on SNU449 hepatocellular carcinoma cell line cancer stem cells is not known. (fabad.org.tr)
  • For virus production Phoenix cells (producer cell line) were splitted (well separated) on 100mm plates. (igem.org)
  • Additionally, poha isolates compounds were evaluated for their growth inhibitory effects with U251MG glioblastoma and MDA-MB-231 breast cancer cells that harbor aberrantly-active signal transducer and activation of transcription 3 (STAT3), compared to normal NIH-3T3 mouse fibroblasts. (nih.gov)
  • Compared to control-treated cells, CD44 (3.2- fold) and p-STAT3 (2.44-fold) protein expressions were upregulated in PEITC-treated cells. (fabad.org.tr)
  • Results of this study suggest that STAT3- mediated upregulation of CD44 leads to the gain of cancer stem cell phenotype of PEITC-treated SNU449 cells. (fabad.org.tr)
  • We also find that expression of CK1ε is able to promote oncogenic transformation of human cells in a β-catenin-dependent manner. (plos.org)
  • The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. (bvsalud.org)
  • One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
  • For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)
  • To transmit MEFs, researches should use trypsin to digest the cells (making them detach from the surface) and transmit 1/5 cells digested into a new dish. (wikipedia.org)
  • Treat cultured mouse fibroblast (NIH 3T3) or osteocyte cells with trypsin to detach the cells from the dish. (neb.com)
  • Increased Na/H antiporter activity has been demonstrated after in vivo chronic metabolic acidosis as well as in vitro acid preincubation of cultured rabbit renal tubule cells. (jci.org)
  • To study the underlying molecular mechanisms of this adaptive increase in Na/H antiporter activity, the present studies examined the effect of low pH media on Na/H antiporter activity and mRNA abundance in cultured renal tubule cells. (jci.org)
  • Na/H antiporter mRNA abundance was also increased in MCT cells with high CO2 preincubation as well as in rat renal cortex with in vivo chronic acid feeding. (jci.org)
  • In contrast to renal epithelia, acid preincubation of NIH 3T3 fibroblasts led to suppression of Na/H antiporter activity. (jci.org)
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Cell cultures exposed to transmissible spongiform agent contaminating some component of culture medium, encephalopathy agents and propagated for 30 passages* especially bovine serum ( 13 ). (cdc.gov)
  • Our recent findings have shown that the widely used NH 4 OH/Triton X-100/DNase decellularization method is not able to completely remove cytoskeletal proteins from long-term three-dimensional human gingival fibroblast cultures. (researchsquare.com)
  • A widely used, alkaline detergent-based decellularization method has been developed for 3D NIH-3T3 mouse embryonic fibroblast cultures 10,11 . (researchsquare.com)
  • It involves incubation of the cultures in an alkaline detergent solution (20 mM NH 4 OH/0.5% Triton X-100, pH=8.0) to lyse cell membranes followed by digestion with DNase to remove DNA. (researchsquare.com)
  • In addition, we have used this method to decellularize 3D cultures of human gingival and skin fibroblasts 14,15 . (researchsquare.com)
  • To this end, primary fibroblasts were seeded at a high density (25,000 cells per cm 2 ) and cultured for 7 days, as originally described for mouse NIH-3T3 cells, to generate 3D cultures 11 . (researchsquare.com)
  • Analysis of the cultures showed that the cells had formed 3-5 cell layers and were surrounded by their own ECM at day 7 14,15 . (researchsquare.com)
  • MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. (wikipedia.org)
  • MEF cells can be cultured in vitro in DMEM medium with 10% FBS. (wikipedia.org)
  • In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
  • Ésta línea es un sistema hospedador in vitro de gran valor para los estudios de transformación de virus oncogénicos, ya que las células 3T3 poseen una elevada sensibilidad a la inhibición por contacto. (bvsalud.org)
  • Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)
  • The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL‑17 includes activation of NF‑ kappa B and JNK, and require TNF receptor‑associated factors 6 (TRAF6) in the signaling pathway. (rndsystems.com)
  • The polyclonal antiserum reacts with human and mouse cyclin C. A polypeptide fragment containing amino acids 290-303 (QGPNGSQNSSYSQS) at the C-terminus of human cyclin C, with the addition of a C-terminal cysteine (C) residue to facilitate conjugation to KLH, was used as immunogen. (bdbiosciences.com)
  • MCF-7 human breast carcinoma (ATCC HTB-22), 293 adenovirustransformed human kidney (ATCC CRL-1673), and NIH/3T3 mouse fibroblast (ATCC CRL-1658) cells are suggested as positive controls. (bdbiosciences.com)
  • The G168-728 antibody recognizes human and mouse MLH1 (80-85 kDa). (bdbiosciences.com)
  • In contrast, the ED 50 values were much higher in untransformed cells, specifically at ∼8.8 μmol/L in GM05757 (primary normal human fibroblast), ∼8.9 μmol/L in HNEpC (primary normal human nasal epithelial), and ∼19.6 μmol/L in NIH/3T3 (mouse embryonic fibroblast) cells. (aacrjournals.org)
  • Cell Culture and Immunoblotting NIH 3T3 mouse fibroblast and HT-1080 human fibrosarcoma cell lines were acquired from American Type Culture Collection (Manassas, VA). Mouse embryonic fibroblasts, derived from pregnant CD-1 mice (Charles River Laboratories, Wilmington, MA), were. (scienza-under-18.org)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • In this combined immunodeficiency (SCID) cancer is low in these species (as it chapter, some aspects of this issue mice, in which the human target is in humans), which renders cancer are discussed. (who.int)
  • that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
  • Melatonin was not toxic in NIH/3T3 primary mouse embryonic fibroblast cells at low doses for 24 and 48 h. (tubitak.gov.tr)
  • low dosages of GO promoted cell growth, while high doses induced cell death. (biomedcentral.com)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
  • Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
  • The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
  • In many cases, successfully mobilization is not effected in the patient and inadequate numbers of hematopoietic stem cells are harvested from these patients using current methods. (justia.com)
  • Various decellularization methods have been described in literature, and they involve removal of cells and intracellular components using various chemical (such as acids, bases, and detergents), biological (such as enzymes, chelation agents, and toxins), or physical (such as freeze-thaw cycling) agents. (researchsquare.com)
  • In order to avoid protease-mediated degradation during processing, decellularization methods also need to include protease inhibitors to inactivate intracellular proteases released during the cell lysis 5,6 . (researchsquare.com)
  • Therefore, decellularization methods need to be tailored distinctly for different cell types. (researchsquare.com)
  • Because cell shape is an infinite dimensional descriptor, the mathematics involved is non-routine. (nist.gov)
  • Fibroblast Embryonic stem cell Jian-ming Xu (2005). (wikipedia.org)
  • The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
  • Cells generate partially reduced forms of oxygen, collectively referred to as "reactive oxygen species" (ROS), during respiration and enzymatic processes. (biomedcentral.com)
  • To uncover genetic dependencies in breast cancer cells that harbor active β-catenin signaling, we performed RNAi-based loss-of-function screens in breast cancer cell lines in which we had characterized β-catenin activity. (plos.org)
  • Furthermore, we demonstrate that breast cancer cells that harbor activated β-catenin activity exhibit enhanced sensitivity to pharmacological blockade of Wnt/β-catenin signaling. (plos.org)
  • consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
  • In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
  • Considering that a host of intracellular proteins are affected by proteasome inhibition, coupled with evidence that knockdown of MKP3 expression enhances growth factor-stimulated ERK phosphorylation in some contexts [13] but not in others [16], led us to question whether or not the diminution of ERK signaling in MG132-treated cells could be attributed solely to upregulation of MKP3 and other DUSPs. (scienza-under-18.org)
  • Overexpression of IRS-1 resulted in inhibition of basal autophagy, and reduced oxidative stress-induced autophagy and cell death. (biomedcentral.com)
  • The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
  • Cells were transduced with MuLV IRES EGFP after 24 h of incubation at 37°C. (igem.org)
  • Cells were transduced with MuLV IRES EGFP after 24 h of incubation at 37°C. This time there were no results du to high density of cells during transduction. (igem.org)
  • Cell viability, colony formation, and wound healing assays were performed to determine proliferative and migratory characteristics. (fabad.org.tr)
  • Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. (hindawi.com)
  • Melatonin inhibited cell growth and induced apoptosis in 5RP7 cells. (tubitak.gov.tr)
  • These findings show that melatonin induces cell death and apoptosis in H-ras oncogene transformed 5RP7 cells. (tubitak.gov.tr)
  • The compound induced cell cycle arrest at low concentrations and apoptosis at higher concentrations. (nih.gov)
  • In addition to inducing necrosis and apoptosis, ROS induces autophagic cell death. (biomedcentral.com)
  • Lines have been developed using several different strains of mice. (bvsalud.org)
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
  • To prepare MEFs, pregnant female mice are needed. (wikipedia.org)
  • Our study provides new information on how Env antigenic differences drastically affect the expansion of particular B cell lineages and supports immunogen-design efforts aiming at stimulating the expansion of cells expressing particular B cell receptors. (plos.org)
  • We report that the variable domains of recombinant HIV-1 Env immunogens activate a large number of B cell clones that give rise to many non-neutralizing antibodies, and that removing the variable domains from the immunogen reduces the number of activated B cell lineages and leads to the development of autologous neutralizing antibodies, a step towards bNAb-production. (plos.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • In contrast, The conclusion that PrPTSE is central to the pathogenesis animals inoculated with cells exposed to the BSE agent of TSE is based on the temporal and anatomic correlations remained asymptomatic. (cdc.gov)
  • Understanding cellular responses to stimuli, such as pharmaceuticals or environmental toxins and discovering correlations between cellular responses and the cell phenotype are applications motivating this work. (nist.gov)
  • The goal of the present study was to investigate whether PEITC regulates the cancer stem cell phenotype of SNU449 cells. (fabad.org.tr)
  • Decellularization allows for the generation of cell and tissue type-specific three-dimensional (3D) cell-derived matrices (CDMs) synthesized and organized by cultured primary cells. (researchsquare.com)
  • This statistical application involves determining the similarity of cell populations based on comparing cell shapes. (nist.gov)
  • Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
  • Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
  • After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
  • The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
  • It is not fully understood how cancer cells grow rapidly and survive in the presence of high ROS levels. (biomedcentral.com)
  • In this short paper, we confirm that MG132 treatment reduces phosphorylation of ERK in fibroblasts stimulated with platelet-derived growth factor (PDGF) or basic fibroblast growth factor (FGF) and show that this is usually caused by two parallel effects. (scienza-under-18.org)
  • Our results suggest that overexpression of IRS-1 promotes cells growth, inhibits basal autophagy, reduces oxidative stress-induced autophagy, and diminishes oxidative stress-mediated autophagy-dependent cell death. (biomedcentral.com)
  • MEFs are widely used in life science researches, especially in stem cell biology. (wikipedia.org)
  • In 2006, Shinya Yamanaka reprogrammed MEFs into iPSCs by introducing 4 factors, which is remarkable in the development of stem cell biology. (wikipedia.org)
  • 2015). Principles of Stem Cell Biology and Cancer: Future Applications and Therapeutics. (wikipedia.org)
  • Cyclin C may play a dual role within the cell in its ability to regulate both cell cycle progression as well as gene transcription. (bdbiosciences.com)
  • Because Cdc2 kinase is important for cell entry into mitosis, cyclin C's ability to regulate cell cycle progression may be attributed, in part, to modulation of Cdc2 protein expression.6 Cyclin C has a predicted molecular weight of 36 kD. (bdbiosciences.com)
  • Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
  • These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
  • Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
  • In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
  • The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
  • Also, using image analysis and visualization software for tracking live cells developed at by the Cell Systems Science Group, we have begun using our results to track, over time, the relationship between cellular characteristics (expressed by GFP intensity) and cell shapes changes. (nist.gov)
  • Western blot analysis of cyclin C in NIH 3T3 mouse fibroblasts. (bdbiosciences.com)
  • Applications include immunoprecipitation (2 µg/1x106 cells) and western blot analysis (1-3 µg/ml). (bdbiosciences.com)
  • Finally digest the remains by enzymes to obtain single isolated cells and culture the cells in a tissue culture dishes. (wikipedia.org)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • BSE scrapie in culture cells with TSE agents from animal-derived Actual or candidate cell lines for vaccine production materials in media has been considered low. (cdc.gov)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • In MCT cells, Na/H antiporter mRNA abundance measured by RNA blots increased by two- to fivefold after 24 h in low [HCO3] media. (jci.org)
  • In the case of afatinib this profile translates into a robust reduction of colony formation and tumor growth of EGFR G724S -driven cells. (nature.com)
  • bNAbs are derived from B cells that express B cell receptors formed by specific VH/VL alleles. (plos.org)
  • 30 µg of 293 cell lysate per lane was probed with 3 µg/ml (lane 1) or 1 µg/ml (lane 2) of anti- MLH1 antibody (clone G168-728). (bdbiosciences.com)
  • Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)
  • Cancer cells produce greater levels of ROS than normal cells do because of increased metabolic stresses. (biomedcentral.com)
  • Nevertheless, researchers can use several strategies, like virus infection or repeated transmission to immortalize MEF cells, which can let MEFs grown indefinitely in spite of some changes in characters. (wikipedia.org)
  • PrPC is a susceptibility to infection of 5 cell lines used or proposed for nonessential protein but, at least in mice and cows, must be manufacture of biological products, as well as other lines. (cdc.gov)
  • Cell expressed by animals susceptible to TSE infection. (cdc.gov)
  • All cell lines we studied resisted between accumulation of PrPTSE and the development infection with 3 TSE agents, including the BSE agent. (cdc.gov)
  • The protocols for decellularization are dependent on cell and tissue type and species of origin usually requiring multiple chemical and biological reagents supplied in a series of incubations 6 . (researchsquare.com)
  • Epithelial Protein Lost in Neoplasm α is a novel cytoskeleton-associated tumor suppressor whose expression inversely correlates with cell growth, motility, invasion and cancer mortality. (biomedcentral.com)
  • For two populations of cells, dissimilarity matrices are created whose entries are geodesic distances between cell shape pairs. (nist.gov)
  • Tissue and cell type-specific cell-free extracellular matrices generated by various decellularization protocols can be important tools to study cell interactions with their distinct extracellular niche. (researchsquare.com)
  • Peripheral blood mononuclear cells of healthy donors and post-transplant lymphoma patients were stimulated with or without lunasin in the presence of IL-12 or IL-2. (weeksmd.com)
  • Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. (bdbiosciences.com)