Mouse fibroblast cell line 3t3. Medical search. Frequent questions

Nonobese diabetic or BALB/c mice were injected i.p. with xenogeneic, allogeneic, or syngeneic cells, and the responding cells in subsequent lavages were assessed by flow cytometry and adoptive transfer. (aai.org)
The analysis was extended to immature lymphocytes of the thymus, and population of thymocytes was identified which was able to form junctions with murine fibroblasts, Swiss 3T3 and a BALB/c 3T3 and the hypoxanthine guanine phosphoribosyl transferase mutant (HGPRT-) subline of BHK/C13 baby hampster kidney fibroblast line TG2. (gla.ac.uk)
Membranes were prepared from the cell line 5ET4 a Balb 3T3 mouse fibroblast cell line expressing a stably transfected mouse GRPr (29). (biopaqc.com)
In ex vivo experiments, tumor growth of BALB/C nude mice breast cancer was retarded after rAAV-2/HSVtk/Tet-On was injected into the tumors under the Dox induction. (biomedcentral.com)
The expression of HSVtk gene in MCF-7 cells and BALB/C nude mice tumors was up-regulated by Tet-On under Dox induction with reverse transcription-PCR (RT-PCR) analysis. (biomedcentral.com)
A permanent mouse fibroblast cell line - Balb/c 3T3, clone 31 - either from ATCC or from ECACC was used in the validation study, and is therefore recommended. (fingerwaverecords.com)
Hungarian Állandó egér fibroblast sejtsort - Balb/c 3T3, 31-es klón -ATCC-ből vagy ECACC-ből, használtak a … Learn more. (fingerwaverecords.com)
Balb/c-3T3 mouse fibroblasts were exposed by 24 hours to the extracts and cell viability was assayed by three parameters: mitochondrial activity (XTT), membrane integrity, measured by Neutral red uptake, and cell density as measured by the Violet Crystal dye exclusion test. (bvsalud.org)

3T3 cells are several cell lines of mouse embryonic fibroblasts. (wikipedia.org)
Cell Culture and Immunoblotting NIH 3T3 mouse fibroblast and HT-1080 human fibrosarcoma cell lines were acquired from American Type Culture Collection (Manassas, VA). Mouse embryonic fibroblasts, derived from pregnant CD-1 mice (Charles River Laboratories, Wilmington, MA), were. (scienza-under-18.org)

SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). (rndsystems.com)
PECAM-1 (Compact disc31) is a glycoprotein expressed in endothelial and bone tissue marrow precursor cells. (bio-cavagnou.info)
Keywords: Compact disc31 Cloning NIH-3T3 Angiogenesis Antibody Launch Individual platelet endothelial cell adhesion Balaglitazone molecule-1 PECAM-1 or Compact disc31 gene includes 16 exons and situated on chromosome 17 in your community 17q23. (bio-cavagnou.info)
In addition it presents in endothelial features and cells in extravasation of Balaglitazone leukocytes angiogenesis and activation of integrins. (bio-cavagnou.info)
Three major types of hCRPs are DAF , MCP , and CD59 , which are expressed mainly by endothelial cells in mammals. (ectrx.org)
Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)
Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)
ETP augmented fibrosis, angiogenesis, and inflammation through recruitment of macrophages and endothelial cells. (jci.org)

Of these some groups have got stably portrayed the gene coding for proteins appealing in mouse fibroblast cell series NIH-3T3 14 and also have utilized the cells as immunogen.15-17 Due to murine origin of NIH-3T3 cell line the just immunogen element of stably transfected cells is normally ectopically portrayed protein. (bio-cavagnou.info)
Viability of NIH 3T3 cells stably transfected with these plasmids was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (ectrx.org)
Fluorescence recovery after photobleaching (FRAP)kinetics of histone H1-zero were recorded using mouse fibroblast cell line stably expressing GFP-H1-zero. (ucsd.edu)

TSEs are characterized by the accumulation in the central nervous system and, less often, in lymphoid tissues of TSE-associated prion protein (PrP TSE ), a conformational variant of a normal host cellular prion protein (PrP C ). PrP C is a nonessential protein but, at least in mice and cows, must be expressed by animals susceptible to TSE infection. (cdc.gov)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
IHC-P: Human uterus, human smooth muscle and mouse smooth muscle tissues. (abcam.com)
Mouse lung and liver tissues. (abcam.cn)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Many of the novel cDNA sequences that were highly represented in the library showed a relative specificity to β-cells compared with other tissues, including glucagonoma, liver, kidney, brain, 3T3 fibroblasts, and AtT20 corticotrophs, and warrant further investigation. (diabetesjournals.org)
While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)
Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. (bvsalud.org)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

WB: Rat kidney and liver tissue lysate, A431, U-2 OS, HepG2 whole cell lysates. (abcam.cn)

Similarly low concentrations (1-100 ng/mL) promoted the susceptibility of porcine macrophages and intestinal epithelial cells to Salmonella Typhimurium invasion, in a SPI-1 independent manner. (idexlab.com)
Gp120 binds to cells expressing CD4 cell-surface antigens, most notably T4-lymphocytes and monocytes/macrophages. (lookformedical.com)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

J. Cell Biol. (wikipedia.org)
Trends Cell Biol. (progen.com)
J Cell Biol (1985) 100 (5): 1415-1422. (silverchair.com)
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). (bvsalud.org)

Using a tetrazolium-based assay, the dose required to reduce cell viability by 50% (ED 50 ) was found to be ∼1.8 μmol/L in FaDu (human hypopharyngeal squamous cancer) and ∼2.6 μmol/L in C666-1 (human undifferentiated nasopharyngeal cancer) cells. (aacrjournals.org)
It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Cell viability, colony formation, and wound healing assays were performed to determine proliferative and migratory characteristics. (fabad.org.tr)
The purpose of this paper is evaluate the cytotoxicity of orthodontic archwires on polymeric resin reinforced with glass fiber, by three different cell viability parameters. (bvsalud.org)
Both archwires (stainless steel and fiber glass reinforced resin) were consisted with high levels of cell viability as measured by the three tests, without significative statistical differences among groups. (bvsalud.org)

Swiss 3T3 cells, newborn foreskin fibroblasts, substrate-attached material ("extracellular matrix"), and tissue culture plastic did not support casein synthesis, whereas monolayers of 3T3-L1 and 3T3-C2 cells, a subclone of Swiss 3T3 cells that does not undergo adipocyte differentiation, did. (silverchair.com)

Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue. (wikipedia.org)
Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. (rndsystems.com)
It has become clear, however, that the susceptibility of cells to infection with TSE agents cannot be reliably predicted either from their tissue of origin or level of expression of PrP C ( 11 , 12 ). (cdc.gov)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Nonvascularized xenografts, such as tissue cultured fetal pig pancreas (FPP) 3 or islets, are not prone to hyperacute rejection by preformed Abs, but are rejected within 10 days by an aggressive cell-mediated response that is difficult to prevent compared with allograft rejection ( 1 , 2 , 3 ). (aai.org)
These responses include: secretion of gastrointestinal human hormones (e.g. gastrin neurotensin cholecystokinin somatostatin and enteroglucagon) rules of smooth muscle tissue contractility modulation of neuronal activity and AM 694 development regulation of regular and neoplastic cells (for review discover ref. 1). (biopaqc.com)
The progress was made in increasing the product of therapeutic genes combined with suicide gene therapy using either tumor cell-specific or tissue-specific promoters. (biomedcentral.com)
Synthetic peptides or recombinant fragments containing bio- logically active motifs have been shown to partially mimic the effects of native laminin in various cellular functions, such as cell adhesion, spreading, migration, neurite outgrowth, and tumor metastasis [6-10], suggesting that laminin-derived-short peptide- modified biomaterials may have therapeutic potential in tissue regeneration applications. (mirnamimic.com)
These effects were partially mediated through enhanced TGF-β signaling, which contributes to tissue fibrosis and epithelial-mesenchymal transition (EMT) of tumor cells. (jci.org)

Mammary epithelium from mid-pregnant mice was plated on confluent irradiated monolayers of 3T3-L1 cells, a subclone of the Swiss 3T3 cell line that differentiates into adipocytes in monolayer culture and other cell monolayers (3T3-C2 cells, Swiss 3T3 cells, and human foreskin fibroblasts). (silverchair.com)

Alix (ALG-2-interacting protein X), a cytoplasmic adaptor protein involved in endosomal sorting and actin cytoskeleton assembly, is required for the maintenance of fibroblast morphology. (nih.gov)

Contact inhibited, a confluent monolayer of 3T3 cells yields approximately 40,000 cells per square centimeter. (fsu.edu)
Adherent cells grow as a monolayer. (wikipedia.org)
A confluent monolayer yields 40000 cells/cm2. (wikipedia.org)
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
Furthermore, T-2 Toxin (1-5 ng/mL) promoted the translocation of Salmonella Typhimurium over an intestinal porcine epithelial cell monolayer. (idexlab.com)

Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. (wikipedia.org)
Later, as a principal investigator position at the National Cancer Institute in Bethesda, Maryland, Todaro repeated the isolation procedure from the NIH Swiss mouse embryo with his students and established NIH-3T3 cell line. (wikipedia.org)

Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
GCV treatment to the rAAV infected MCF-7 cells was performed by MTT assay under the doxycycline (Dox) induction or without Dox induction at a vp (viral particle) number of ≥10 4 /cell. (biomedcentral.com)
Finally, the in vitro antiproliferative activity of all the complexes was evaluated against human cervical cancer (HeLa) and mouse embryonic fibroblast (NIH-3T3) cell lines by MTT assay and the results suggested that the complexes were significantly active compared to various clinically reported drugs. (uniss.it)
This assay system using XBF cells cocultured with irradiated 3T3 fibroblast feeder cells was examined as a possible in vitro screening assay for dioxinlike activity by testing benzene extracts of soot from a fire involving a PCB-containing transformer. (cdc.gov)
This comparison showed a good correlation, suggesting that the XBF-3T3 system has potential for use as a semiquantitative assay for dioxinlike activity. (cdc.gov)

Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
Although the precursor protein of NFκB2 (p100) is thought to act as a tumor suppressor in mammalian cells, the molecular mechanism of its anti-tumor activity is far from clear. (oncotarget.com)
NFκB1(p50) and NFκB2(p52) are synthesized as large precursors of p105 and p100, respectively in mammalian cells [ 1 ]. (oncotarget.com)
While RelA/p50 heterodimer is the predominantly form and plays a major regulatory role in mammalian cells, the RelB/p100 is also expressed and acts as the alterative pathway regulating its downstream gene expression [ 2 ]. (oncotarget.com)
Although the diverse tumor-promoting roles of NFκB in cancer cell proliferation, anti-apoptosis, angiogenesis, invasion and metastasis, are well established [ 3 - 9 ], much less is known about how p100, a precursor protein of NFκB2, acts as a tumor suppressor in many mammalian cells [ 10 ]. (oncotarget.com)
Domke, L and Franke, W. The cell-cell junctions of mammalian testes. (progen.com)

PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse SPARC Monoclonal Antibody (Catalog # MAB942) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005 ). (rndsystems.com)
Antibody binding to extracellular Alix retards WI38 cell adhesion and spreading on fibronectin and vitronectin. (nih.gov)
When using antibody 2G2 in immunolocalization studies the presence of nuclear actin could be demonstrated e.g. in cultured cells after fixation with 4% paraformaldehyde. (progen.com)
Mouse monoclonal anti-human Compact disc31 antibody (Biolegend London UK) was added on cells in last focus of 5 μg/ml. (bio-cavagnou.info)
In parallel cells had been stained with isotype control antibody (Biolegend London UK) as detrimental control. (bio-cavagnou.info)

Medullary epithelial cells (mTEC), in conjunction with dendritic cells, then take over control of developing thymocytes. (frontiersin.org)

The ubiquitin-proteasome pathway is an essential quality control mechanism directing degradation of mislocated, misfolded, and damaged proteins, and, by tempering the expression levels Carnosol of specific signaling proteins, it also exerts a level of control over cell physiology [1]. (scienza-under-18.org)
Considering that a host of intracellular proteins are affected by proteasome inhibition, coupled with evidence that knockdown of MKP3 expression enhances growth factor-stimulated ERK phosphorylation in some contexts [13] but not in others [16], led us to question whether or not the diminution of ERK signaling in MG132-treated cells could be attributed solely to upregulation of MKP3 and other DUSPs. (scienza-under-18.org)
Knockdown of p100 in cultured human bladder cancer cells promoted anchorage-independent growth accompanied with elevating abundance of cell-cycle-related proteins and accelerated cell-cycle progression. (oncotarget.com)
Furthermore transfection of cDNA Balaglitazone coding for a particular proteins in NIH-3T3 cell series continues to be performed for reasons apart from immunization of mice e.g. the signaling functional or potential properties from the molecule.18-21 Here we reported cloning of individual Compact disc31 cDNA and steady expression in NIH-3T3 mouse fibroblast cell line for forthcoming experiments to create monoclonal antibodies against Compact disc31. (bio-cavagnou.info)
To analyze the protective effects against complement-mediated cytolysis of the MCP , DAF , and CD59 human complement regulatory proteins, alone and in combination, on NIH 3T3 mouse fibroblast cells. (ectrx.org)
Adipocyte-derived factors involved in tumor progression include proteins such as adiponectin, leptin, TNF-α, monocyte chemotactic protein-1 (MCP-1), IL-6, and ECM components that control tumor cell behavior within the tumor microenvironment. (jci.org)

However, due to examination of 3T3 cells and subsequent research it has become widely accepted that for immortalization of cells to take place, telomere shortening, which can instigate chromosomal rearrangements, must be overcome, a process that is not necessarily related to a cell s ability to undergo oncogenic transformation. (fsu.edu)
The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. (bvsalud.org)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)

Flow Cyt (intra): HeLa cells. (abcam.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)

At the time of their establishment, 3T3 cells were different than most other cell lines in regard to the fact they did not induce tumors to develop when injected into murine species. (fsu.edu)
We have evaluated the antitumor effect of HSVtk/ganciclovir gene therapy under Tet-On regulation by means of adeno-associated virus-2 (AAV-2)-mediated HSVtk gene transfer with direct intratumoral injections in mice bearing breast cancer tumors. (biomedcentral.com)
Infiltrating cells were also observed in tumors after Dox induction followed by GCV treatment and cells were profoundly damaged. (biomedcentral.com)
Divided into five groups of six animals in each the same fibroblast clones Fibroadenomas are benign tumors. (fingerwaverecords.com)
Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice. (lookformedical.com)

Mammary epithelium differentiates in a stromal milieu of adipocytes and fibroblasts. (silverchair.com)
These microenvironments are classically defined as distinct cortex and medulla regions that each contain specialized subsets of stromal cells. (frontiersin.org)
While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)
Arguably, the most recognized and well-studied stromal populations in thymus biology are thymic epithelial cells (TEC) within the cortical and medullary areas, which are defined by their anatomical separation and function. (frontiersin.org)
Our results highlight the crucial role of ETP as an obesity-associated factor that promotes tumor growth in the context of adipocyte interactions with tumor and stromal cells. (jci.org)

Peitsch, W. K. Cell biological and biochemical characterization of drebrin complexes in mesangial cells and podocytes of renal glomeruli. (progen.com)

This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
Na/H antiporter activity was increased by 60% in a mouse renal cortical tubule cell line (MCT), and by 90% in an opossum kidney cell line (OKP) after 24 h of preincubation in acid (low [HCO3]) media. (jci.org)

The primary aim of this research was to examine the ability of lymphoid cells to form permeable intercellular communicating junctions (gap junctions). (gla.ac.uk)
However, there is variability in the expression of Thy-1 and H-2K antigens and the frequency of thymocytes that can form junctions is of the order of 5x10-4 and 10-5 cells. (gla.ac.uk)

As Alix has sequence similarity to adhesin in Entamoeba histolytica, and we observed that Alix is secreted, we determined whether extracellular Alix affects fibroblast morphology. (nih.gov)
In vitro bioassay for dioxinlike activity based on alterations in epithelial cell proliferation and morphology. (cdc.gov)
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to induce changes in morphology and proliferation characteristics of a nonkeratinizing derivative (XBF) of a keratinizing epithelial cell line (XB), cloned from a mouse teratoma, when cocultured with irradiated feeder cells. (cdc.gov)

In two experimental models of IBD, genetic ablation of ST2 significantly improved signs of colitis, while a sustained epithelial expression of the cyto-protective factor connexin-43 was observed in DSS-treated St2 -deficient mice. (bmj.com)
Accordingly, cells treated with MG132 or other proteasome inhibitors exhibit higher expression of MKP3/DUSP6, an ERK1/2-specific DUSP, accompanied by lower levels of ERK phosphorylation stimulated by growth factors [12]C[14]. (scienza-under-18.org)
Here, we are, for the first time, to report that p100 protein expression was dramatically decreased in bladder cancers of N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN)-treated mice and human patients. (oncotarget.com)
Importantly, enhanced cellular behavior and membrane translocation of PKCd induced by Go¨6976 was retained in siRNA-transfected PC12 cells to knockdown PKCa expression. (mirnamimic.com)
siRNA knockdown of Rac1 and Cdc42 expression inhibited cell spreading and lamellipodia formation in PC12 cells. (mirnamimic.com)
of NIH-3T3 cells using 3 μg of recombinant build and 6 μl of JetPEI transfection reagent steady expression was attained by collection of cells by G418 antibiotic and verified by surface stream cytometry. (bio-cavagnou.info)
Transient and steady expression of individual Compact disc31 on transfected NIH-3T3 mouse fibroblast cells was attained (23% and 96% respectively) as proven by stream cytometry. (bio-cavagnou.info)
Stream cytometry Evaluation of surface area expression of Compact disc31 molecule on KG1a being a supply for cloning of individual Compact disc31 was performed by indirect staining of KG1a cells. (bio-cavagnou.info)
In addition, mTEC screen thymocytes for their expression of high affinity αβ-TCRs, with negative selection limiting the release of these reactive T-cells into the peripheral pool ( 4 ). (frontiersin.org)

In experiments probing for a role of PKCa in human laminin-2-integrin-mediated cell adhesion and spreading of PC12 cells, we observed unexpected enhancements of adhesion, spreading and stress fiber formation to 1 mM Go¨6976 with concomitant increase in membrane translocation of PKCd and autophosphorylation of focal adhesion kinase (FAK). (mirnamimic.com)
A specific inhibitor of PKCd, rottlerin, completely abrogated Go¨6976-dependent increase in PC12 cell adhesion to laminin as well as the activation of small GTPases, Rac1 and Cdc42, that are downstream of PKCd in adhesion receptor signaling. (mirnamimic.com)
FAK signaling mediates cell spreading and lamellipodia formation in PC12 cells. (mirnamimic.com)
In PC12 cells, we recently showed that the engagement of integrin a3b1 to a binding sequence within the human laminin a2 chain was sufficient to recruit PKCa and PKCd to the integrin receptor [6]. (mirnamimic.com)

Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)
Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)
In contrast, the ED 50 values were much higher in untransformed cells, specifically at ∼8.8 μmol/L in GM05757 (primary normal human fibroblast), ∼8.9 μmol/L in HNEpC (primary normal human nasal epithelial), and ∼19.6 μmol/L in NIH/3T3 (mouse embryonic fibroblast) cells. (aacrjournals.org)
1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
1991. Genetic activity of the human carcinogen sulphur mustard towards salmonella and the mouse bone marrow. (cdc.gov)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
In this combined immunodeficiency (SCID) cancer is low in these species (as it chapter, some aspects of this issue mice, in which the human target is in humans), which renders cancer are discussed. (who.int)

Variants of the initial cell line have been tested and found negative for ectromelia virus (mousepox), but most are susceptible to polyoma and simian virus 40 ( SV40 ). (fsu.edu)
3T3 cells can be transformed with SV40 and some other polyomaviruses. (wikipedia.org)

The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
Wild-type and St2 −/− mice were used in wound healing experiments and in two experimental models of IBD triggered by 2,4,6-trinitrobenzene sulphonic acid or dextran sodium sulphate (DSS). (bmj.com)
In preliminary experiments examining the role of PKCa in cell adhesion and spreading, we found that Go¨6976, a widely used, nonglycosidic indolocarbazole inhibitor of PKCa/b, had effects that could not be explained by inhibition of PKCa/b alone. (mirnamimic.com)

In the case of afatinib this profile translates into a robust reduction of colony formation and tumor growth of EGFR G724S -driven cells. (nature.com)
Adipocytes represent a major cell type in the mammary tumor microenvironment and are important for tumor growth. (jci.org)

Because of murine origins of NIH-3T3 cell series Compact disc31-expressing NIH-3T3 cells could possibly be useful as immunogen in creation of diagnostic monoclonal antibodies against individual CD31 without the need for Balaglitazone purification of recombinant protein. (bio-cavagnou.info)

Increased Na/H antiporter activity has been demonstrated after in vivo chronic metabolic acidosis as well as in vitro acid preincubation of cultured rabbit renal tubule cells. (jci.org)
Ésta línea es un sistema hospedador in vitro de gran valor para los estudios de transformación de virus oncogénicos, ya que las células 3T3 poseen una elevada sensibilidad a la inhibición por contacto. (bvsalud.org)

9 translations and more for fibroblasts objects, keratin and suture. (fingerwaverecords.com)

proteasome inhibition interferes with cell cycle progression, upregulates tumor suppressors such as p53, and diminishes activation of pro-proliferation pathways such as those controlled by NFB and extracellular signal-regulated kinases (ERKs) [3], [4]. (scienza-under-18.org)

MTT analyses results revealed the safety of the complexes on 3T3 mouse fibroblast cell lines while Microbroth Dilution Method revealed the remarkable antifungal activities of the complexes. (anadolu.edu.tr)

Many investigators once believed that TSE agents infected mainly, if not exclusively, cells of neuronal and lymphoid lineages. (cdc.gov)
Established techniques, based on the detection of intercellular exchange of radiolabelled nucleotides, were used to analyse the junction formation between the adherent lymphoid tumour cell line RAJI and other cell lines of known junction forming ability. (gla.ac.uk)
The results show no detectable junction formation between PBL and a range of junction-forming cell lines and the adherent lymphoid line RAJI. (gla.ac.uk)

In contrast to renal epithelia, acid preincubation of NIH 3T3 fibroblasts led to suppression of Na/H antiporter activity. (jci.org)

Species of GAMMARETROVIRUS , containing many well-defined strains, producing leukemia in mice. (lookformedical.com)
Lines have been developed using several different strains of mice. (bvsalud.org)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

Compact disc31 is portrayed on several cells including monocytes polymorphonuclears (PMNs) platelets plus some subsets of T lymphocytes. (bio-cavagnou.info)

Because cell shape is an infinite dimensional descriptor, the mathematics involved is non-routine. (nist.gov)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)

In addition to intrathymic selection, and during this medullary residency period, CD4 + and CD8 + single positive (CD4SP, CD8SP) thymocytes acquire the ability to proliferate in response to TCR stimulation, and undergo licensing for cytokine production, prior to exiting the thymus to join the peripheral T-cell pool as Recent Thymus Emigrants (RTE) ( 5 , 6 ). (frontiersin.org)

In this short paper, we confirm that MG132 treatment reduces phosphorylation of ERK in fibroblasts stimulated with platelet-derived growth factor (PDGF) or basic fibroblast growth factor (FGF) and show that this is usually caused by two parallel effects. (scienza-under-18.org)
Pharmacologic blockade of PKC inhibits cell adhesion and spreading as well as focal adhesion formation and focal adhesion kinase (FAK) phosphorylation [13,14]. (mirnamimic.com)

We used three nonparametric tests, the Friedmann-Rafsky Minimum Spanning Tree test, the Schilling Nearest Neighbor test and an energy test to test the hypothesis that two cell populations are statistically equivalent. (nist.gov)

AM 694 The swollen cells were harvested by scraping and homogenized in a Dounce homogenizer (15-20 strokes with the tight pestle) and the nuclei and cell debris were removed by centrifugation at 750 × for 10 min at 4°C. The postnuclear membrane fraction (P2) was collected from the supernatant by centrifugation at 75 0 × for 30 min at 4°C. Chaotropic Extraction of Endogenous GTP Binding Activity. (biopaqc.com)

Anatomy35

Organisms22

Diseases10

Chemicals and Drugs82

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes74

Disciplines and Occupations1

Information Science3

Health Care3

BALB8

Embryonic fibroblasts2

Endothelial8

Stably3

Tissues10

A4311

Macrophages3

Biol4

Viability5

Extracellular Matrix1

Tissue15

Subclone1

Actin cytoskeleton1

Monolayer5

Swiss5

Assay5

Mammalian6

Antibody5

Dendritic cells1

Proteins6

Oncogenic4

Hela3

Tumors5

Stromal5

Characterization1

Kidney2

Junctions2

Morphology3

Expression9

PC124

Human7

SV402

Experiments3

Tumor growth2

Immunogen1

Vitro2

Keratin1

Proliferation1

Complexes1

Lymphoid3

Suppression1

Strains3

Platelets1

Descriptor1

Recombinant monoclonal1

Undergo1

Phosphorylation2

Hypothesis1

Nuclei1