• FGF15 is found in the absorptive cells of the mouse ileum and plays an important role in feedback inhibition of hepatic bile acid synthesis. (wikipedia.org)
  • Changes in hepatic Cyp7a1 messenger RNA (mRNA) correlated with those of Cyp24a1 (a Vdr target gene) and inversely with Shp mRNA, but not ileal Fgf15 mRNA. (nih.gov)
  • Activation of the VDR represses hepatic SHP to increase levels of mouse and human CYP7A1 and reduce cholesterol. (nih.gov)
  • In this study, the effect of dietary protein restriction on hepatic VLDLR and the role of VLDLR in fatty liver were investigated using Vldlr knockout (KO) mice. (bvsalud.org)
  • In WT mice, the amount of hepatic Vldlr mRNA and VLDLR protein increased by approximately 8- and 7-fold, respectively, due to protein restriction. (bvsalud.org)
  • In contrast, none on the hepatic FXR target genes appear to become affected by 15. (nicotinic-receptor.com)
  • To explore new functions of FXR, we examined hepatic binding sites for FXR in the entire mouse genome by ChIP-seq analysis and were able to identify previously unknown functions of FXR, including hepatic autophagy. (illinois.edu)
  • EMBO J, 2017), we found that the fed-state sensing nuclear receptor FXR and the fasting transcriptional activator CREB coordinately regulates the hepatic autophagy gene network and that CREB and FXR oppositely regulate autophagic degradation of lipid (lipophagy). (illinois.edu)
  • A micro array profile of intestinal, liver and fat tissue of GDNP treated mice was used to analyze their gene expression profile. (thno.org)
  • Increasing expression of Foxa2 leads to altering the composition of intestinal epithelial cell (IEC) exosomes of mice fed a HFD and prevents IEC exosome mediated insulin resistance. (thno.org)
  • FXR can also indirectly regulate gene expression in the liver via FXR upregulation of intestinal FGF15/19. (metacrine.com)
  • Expression of FXR target genes in C57BL/6N mouse ileum. (nicotinic-receptor.com)
  • Shp and Fgf15 as FXR target genes had been potently down-regulated by the nonsteroidal antagonist 15 at 10 and 30 mg/kg, comparable towards the outcomes obtained with all the steroidal antagonist eight.14 Conversely, the Asbt gene was induced by 15, indicating that the three genes within the ileum are coordinated by 15 (Figure 4). (nicotinic-receptor.com)
  • Variations in regulation by 15 noticed in each organ imply that it specifically exerts an effect on the target genes in the ileum in lieu of the liver. (nicotinic-receptor.com)
  • As a result, we concluded that 15 controls Shp, Fgf15, and Asbt by way of FXR antagonism in the ileum. (nicotinic-receptor.com)
  • In lean male C57Bl/6 mice, evidence of direct FXR engagement in ileum and liver was measured 8 hours post FXR agonist dosing by qPCR analysis of FGF15 in ileum and SHP in liver. (metacrine.com)
  • Expression of FXR target genes in C57BL/6N mouse liver (n = six).CD solution). (nicotinic-receptor.com)
  • FXR agonists were also evaluated in a diet-induced NASH model in which male C57BL/6 mice were fed a diet containing 40% fat, 20% fructose and 2% cholesterol. (metacrine.com)
  • Fibroblast growth factor 15 is a protein in mouse encoded by the Fgf15 gene. (wikipedia.org)
  • FGF2 , also known as basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene . (wikidoc.org)
  • The mouse Fgf15 gene is syntenic with the human FGF19 gene. (wikipedia.org)
  • FGF15 and FGF19 proteins share about 50% amino acid identity, are found in the same tissues, and have similar functions in mouse and humans. (wikipedia.org)
  • FGF15 (and FGF19 in humans) function as hormones produced in response to bile acid absorption acting on the farnesoid X receptor FXR, are secreted into the portal venous circulation and bind onto the liver membrane receptor FGFR4/β-Klotho and repress bile acid synthesis by the Cyp7a1 gene. (wikipedia.org)
  • We first confirmed the presence of Vdr mRNA and protein expression in livers of mice. (nih.gov)
  • Similarly, incubation with 1,25(OH)2D3 increased levels of Cyp24a1/CYP24A1 and Cyp7a1/CYP7A1 mRNA in mouse and human hepatocytes, and reduced levels of Shp mRNA in mouse hepatocytes. (nih.gov)
  • We studied the regulation of Shp by Vdr using reporter and mobility shift assays in transfected human embryonic kidney 293 cells, quantitative polymerase chain reaction with mouse tissues and mouse and human hepatocytes, and chromatin immunoprecipitation assays with mouse liver. (nih.gov)
  • FGF15 has effects on energy homeostasis. (wikipedia.org)
  • Here, we report that treatment with orally administered ginger-derived nanoparticles (GDNP) prevents insulin resistance by restoring homeostasis in gut epithelial Foxa2 mediated signaling in mice fed a high-fat diet (HFD). (thno.org)
  • Growing wild-type (WT) and KO mice were fed a control diet containing 20% â â protein or a low protein diet containing 3% protein for 11 days. (bvsalud.org)
  • Increased liver triglyceride levels during protein restriction occurred in KO mice to the same extent as in WT mice, indicating that increased VLDLR during protein restriction was not the main cause of fatty liver, which was different from the case of ER stress. (bvsalud.org)
  • In addition, protein malnutrition increased expression of the thermogenic gene uncoupling protein 1 in inguinal white adipose and brown adipose tissue in an FGF21-dependent manner. (bvsalud.org)
  • However, surgically removing inguinal white adipose tissue did not affect liver triglyceride levels in protein-malnourished mice. (bvsalud.org)
  • For example, deacetylation of SREBP-1c decreases its gene activity by inhibiting DNA binding and promoting protein degradation. (illinois.edu)
  • A screen for genes involved in endosome-to-TGN trafficking produced two hits, the adaptor protein-1 (AP-1 complex), which facilitates vesicle budding, and WDR11. (cam.ac.uk)
  • Gene sequencing revealed that this group was in fact the same FGF2 protein and that it was a member of a family of FGF proteins. (wikidoc.org)
  • Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene . (wikidoc.org)
  • The protein encoded by this gene is a polypeptide hormone and neurotrophic factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations including astrocytes . (wikidoc.org)
  • Receptor tyrosine-protein kinase erbB-2 , also known as CD340 (cluster of differentiation 340), proto-oncogene Neu , Erbb2 (rodent), or ERBB2 (human), is a protein that in humans is encoded by the ERBB2 gene. (iiab.me)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse EGF Like Domain Protein, Multiple 7 (EGFL7) in serum, plasma, tissue homogenates and other biological fluids. (gkts.net)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse EGF Like Domain Protein, Multiple 7 (EGFL7) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (gkts.net)
  • Description: A sandwich ELISA kit for detection of EGF Like Domain Protein, Multiple 7 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (gkts.net)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Dickkopf Related Protein 4 (DKK4) in serum, plasma and other biological fluids. (gkts.net)
  • Gene expression profiles obtained without artifacts introduced by isolation procedures or gene mutations provide a valuable overview of retina development under natural developmental conditions. (biomedcentral.com)
  • One of our groundbreaking discoveries was the identification of founder mutations in the DYRK1B gene, underlying atherosclerosis, metabolic syndrome, and fatty liver disease. (yale.edu)
  • One of our recent groundbreaking discoveries was the identification of novel loss of function mutations in a gene encoding the pancreatic exocrine elastase Cela2a in patients with diabetes, CAD and MetS traits, including obesity, hypertension, hypertriglyceridemia, NAFLD (OMIM: AOMS4). (yale.edu)
  • Our studies in mice have shown that this upregulation results in mTOR activation, lipogenesis and development of NASH and dyslipidemia. (yale.edu)
  • Here we examined expression patterns of several chick orthologs of LIM-homeobox genes. (ehu.eus)
  • These genes are absent from Av-DJ but show the heinousest exceedingly of distinctiveness to orthologs from several Pseudomonas species though they are not all contiguously clustered ladose akosua pws rifles for sale in these organisms as in pAcX50f. (upb.ro)
  • Molecular cloning of the gene showed that HER2, Neu, and ErbB-2 are all encoded by the same orthologs. (iiab.me)
  • In a murine diet-induced mouse model of biopsy-confirmed NASH, FXR agonists with systemic activity appear more efficacious than FXR agonists that preferentially target FXR receptors in the GI tract. (metacrine.com)
  • In addition, the expression amount of the FXR target genes in the liver, bile salt export pump (Bsep),33 Cyp7a1,three and Shp3 was also examined and depicted in Figure five. (nicotinic-receptor.com)
  • Activation of farnesoid X receptor (Fxr, Nr1h4) is a major mechanism in suppressing bile-acid synthesis by reducing the expression levels of genes encoding key bile-acid synthetic enzymes (e.g., cytochrome P450 [CYP]7A1/Cyp7a1 and CYP8B1/Cyp8b1). (omeka.net)
  • Isolation and characterization of alternative messenger RNAs coding class I HLA genes. (cnr.it)
  • In a mouse model of chronic diarrhea due to bile acid malabsorption, FGF15 administration, or stimulation of its production, reduced the bile acid loss by inhibiting new synthesis. (wikipedia.org)
  • Reducing isoleucine or valine rapidly restores metabolic health to diet-induced obese mice. (bvsalud.org)
  • We propose that large-scale changes in gene regulation during development are necessary for the final maturation and function of the retina. (biomedcentral.com)
  • Revealing the intrinsic program of gene regulation that accompanies mammalian retinal development is a key step for eventual cure of many human retina diseases and blindness. (biomedcentral.com)
  • Brain morphogenesis requires precise regulation of multiple genes to control specification of distinct neural progenitors (NPs) and neuronal production. (frontiersin.org)
  • Knockdown of microRNA (miRNA) miR-7 can rescue abnormal brain morphology in Gli3 knockout mice by recovering progenitor specification, neuronal production and migration through a counter-balance of the Gli3 activity. (frontiersin.org)
  • Fgf15-knock-out mice have reduced liver glycogen storage and are glucose-intolerant. (wikipedia.org)
  • FGF15 has been implicated in liver regeneration and repair. (wikipedia.org)
  • In mice fed normal diets and given injections of 1,25(OH)2D3, liver and plasma concentrations of 1,25(OH)2D3 increased and decreased in unison. (nih.gov)
  • Further, to identify global SHP binding sites and its novel functions in mouse liver, we performed genome-scale ChIP-seq and RNA-seq studies. (illinois.edu)
  • Relative liver and gastrointestinal FXR activity can be monitored via specific gene expression changes. (metacrine.com)
  • Role of liver-enriched transcription factors and nuclear receptors in regulating the human, mouse, and rat NTCP gene[J]. Am J Physiol Gastrointest Liver Physiol, 2004, 286(5):752-761. (magtechjournal.com)
  • Carson MD, Warner AJ, Geiser VL, Hathaway-Schrader JD, Alekseyenko AV , Marshall J, Westwater C , Novince CM . Prolonged Antibiotic Exposure during Adolescence Dysregulates Liver Metabolism and Promotes Adiposity in Mice. (musc.edu)
  • Genes associated with metabolism or insulin signaling were further quantified using the real time polymerase chain reaction (RT-PCR). (thno.org)
  • This study provides a complementary genome-wide view of common gene dynamics and a broad molecular classification of mouse retina development. (biomedcentral.com)
  • Molecular mechanism controlling the expression of the HOX Complex gene family in human teratocarcinoma cells (N-tera2/D1). (cnr.it)
  • Subsequent molecular and physiological studies in human mutation carriers and animal models have allowed us to unravel novel functions of the identified genes, to delineate their cognate pathways and to discover new targets for pharmaceutical intervention. (yale.edu)
  • Isolation and identification of the structural organization of the human homeobox gene family (HOX Complex). (cnr.it)
  • There is no human FGF15. (wikipedia.org)
  • We studied these processes in mice in vivo and mouse and human hepatocytes. (nih.gov)
  • Expression analysis of several Hox genes in human embryos. (cnr.it)
  • Mouse FGF18 / FGF-18 derived in Human Cells. (creativebiomart.net)
  • At birth, we are colonized by a collection of microorganisms, including bacteria, fungi, viruses and archaea, that outnumber our human cells by 10:1 and provide 100-fold as many genes as found in our human genome [ 1 ] . (encyclopedia.pub)
  • By recruiting more than thousand kindreds with early onset CAD and multiple metabolic risk factors for genetics and metabolic studies we have been able to map, identify and characterize a dozen of human disease genes for CAD and MetS, which have been reported in leading journals such as Nat Genet, Science, NEJM, Cell Metab, JCI, PNAS, AJHG, etc. (yale.edu)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Ras Homolog Gene Family, Member A (RHOA) in samples from tissue homogenates, cell lysates or other biological fluids. (hiv-pharmacogenomics.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Ras Homolog Gene Family, Member A (RHOA) in Tissue homogenates, cell lysates and other biological fluids. (hiv-pharmacogenomics.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Ras Homolog Gene Family, Member A (RHOA) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (hiv-pharmacogenomics.org)
  • In vivo dissection of Otx1 and Otx2 roles in brain development by using reciprocal knock-in mouse models: definition of extended functional equivalence between OTX1 and OTX2 proteins and the relevance of the Otx1 and Otx2 differential transcriptional control. (cnr.it)
  • This include the effects of inflammatory diseases of the intestine causing malabsorption, such as coeliac disease and Crohn's disease, and the changes in gene expression during active disease and the repair process. (imperial.ac.uk)
  • Third, even using currently available comprehensive arrays, tissue-specific genes are still under-represented, including alternatively spliced variants or genes that are only briefly expressed in a tissue-specific pattern. (biomedcentral.com)
  • Cerwenka A, Baron JL, Lanier LL (2001) Ecthesis expackion of buy ladose online no prescription retinoic acid ancient inducible-1 gene (RAE-1) permits true murderer apartment-mediated rejection of a MHC catefadery I-bearing tumor in vivo. (upb.ro)
  • We then proceed to characterize the identified genes in vivo and in vitro . (yale.edu)
  • Previous researches have shown that LIM-homeobox genes are important transcription factors during diencephalon regionalization in mice. (ehu.eus)
  • Identification of transcription and processing signals of ribosomal genes of D. melanogaster. (cnr.it)
  • Within the same functional classes, however, different gene populations were expressed in distinct developmental phases. (biomedcentral.com)
  • Different genes in the same functional clusters are expressed in the different developmental stages, suggesting that cells might change gene expression profiles from differentiation to maturation stages. (biomedcentral.com)
  • Role of Otx1 and Otx2 in brain evolution: functional equivalence between the invertebrate Orthodenticle and the murine OTX1 and OTX2 gene products supports a conserved genetic program of brain development between insects and mammals. (cnr.it)
  • Despite focusing on functional studies of correlatively pathogenic genes, less research has been done to investigate possibilities of their counter-regulatory genes as potential modulators of brain morphogenesis and therapeutic targets of malformation. (frontiersin.org)
  • We leverage modern techniques of functional genomics, epigenetics, transcriptomics, proteomics, gene editing and model-driven experimentation to understand the underlying causes of atherosclerosis and metabolic syndrome and discover therapeutic targets. (yale.edu)
  • Between embryonic day 12 and postnatal day 21, six major neuronal and one glia cell type are generated from multipotential progenitors in a characteristic sequence during mouse retina development. (biomedcentral.com)
  • Genes & Development. (wikipedia.org)
  • Investigators at the Max Planck Institute of Biochemistry led by Matthias Mann aimed to capture this picture by using a sensitive and in-depth mass spectrometry approach to profile the aortas of atherosclerotic mice over the development of the disease. (asbmb.org)
  • Six individual gene expression clusters were identified based on expression patterns of transcripts through retina development. (biomedcentral.com)
  • Among 4,180 transcripts that changed significantly during development, approximately 2/3 of the genes were expressed at high levels up until PN5 and then declined whereas the other 1/3 of the genes increased expression from PN5 and remained at the higher levels until at least PN21. (biomedcentral.com)
  • A correlation coefficient analysis of gene expression during retina development between previous SAGE studies and this study was also carried out. (biomedcentral.com)
  • Our data suggest that the LIM-homeobox genes, Lhx1 and Lhx9 , regulated by ventral and/or dorsal signals, may play important roles in controlling regionalization of the diencephalon during chick development. (ehu.eus)
  • Isolation of the murine homologs of the otd and ems homeobox containing genes and analysis of their expression during brain development. (cnr.it)
  • Moreover, miR-7 likely exerts its function through silencing target gene Pax6. (frontiersin.org)
  • Jacqueline Stöckli and colleagues from the University of Sydney carried out a comprehensive machine learning-enabled metabolomic analysis of three mouse strains fed high-fat or standard diets. (asbmb.org)
  • In addition, chromatin immunoprecipitation analysis of livers from mice showed that injection of 1,25(OH)2D3 increased recruitment of Vdr and rodent retinoid X receptor to the Shp promoter. (nih.gov)
  • Isolation and expression analysis both in invertebrate and vertebrate embryos of a new homeobox containing gene named Orthopedia (Otp). (cnr.it)
  • Isolation and expression analysis in murine embryos of Dlx5 and Dlx6, two murine homologues of the Drosophila Distal-less gene. (cnr.it)
  • Our work involves population and family-based genetic studies, high throughput sequencing to identify disease genes, with a focus on coronary artery disease (CAD) and metabolic syndrome (MetS). (yale.edu)
  • ERBB is abbreviated from erythroblastic oncogene B, a gene isolated from avian genome. (iiab.me)
  • Based on FGF15 and SHP gene expression, M790 was the most GI selective FXR agonist, M450 had the highest systemic FXR activity and M780 had intermediate activity (see table). (metacrine.com)
  • Eleven stages of mouse retina, from embryonic day 12.5 (El2.5) to postnatal day 21 (PN21), were collected for RNA isolation. (biomedcentral.com)
  • Most recently, we have established techniques of high throughput gene editing and multiple parallel reporter assays in my laboratory and have successfully mapped the regulatory landscape of a number of GWAS disease genes. (yale.edu)
  • Dysregulation of these genes results in severe brain malformation such as macrocephaly and microcephaly. (frontiersin.org)
  • A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease . (wikidoc.org)
  • Description: A sandwich ELISA kit for detection of Ras Homolog Gene Family, Member A from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (hiv-pharmacogenomics.org)
  • Description: A sandwich ELISA kit for detection of Integrin Beta 6 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (antibody-tech.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse Calpain 1, Large Subunit (CAPN1) in samples from tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (clinical-trial-logistics.com)
  • At 32 weeks, mice underwent biopsy to histologically confirm NASH and obtain a baseline NAFLD activity score (NAS). (metacrine.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Integrin Beta 6 (ITGb6) in Tissue homogenates, cell lysates and other biological fluids. (antibody-tech.com)
  • Identification of an Otx gene-dosage dependent mechanism required for patterning of developing brain and proper positioning of midbrain-hindbrain isthmic organizer. (cnr.it)
  • [9] It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems. (wikidoc.org)