VitroBovine embryoEmbryogenesisBlastocystPrimordial germPost-implantationCryopreservationPluripotentUndergoesSpermPatternsEmbryonicZygoticDemethylationRemethylationFertilizationPaternalChromatinMethylationTransgenicMorulaSyntheticDevelopmentPregnancyVivoHistoneOutcomesTrophectodermStemCellsOccursEarly mouseTransferActivityMalePositionIncreaseDaysEnergy
Vitro9
- In vitro manipulation of pre-implantation embryos has been shown to disrupt methylation patterns at imprinted loci and plays a crucial role in cloned animals. (wikipedia.org)
- A recently published Position Statement (PS) by the Preimplantation Genetics Diagnosis International Society (PGDIS) regarding utilization of preimplantation genetic testing for aneuploidy (PGT-A) in association with in vitro fertilization (IVF) contained inaccuracies and misrepresentations. (biomedcentral.com)
- Here presented consensus offers an alternative to the 2019 PGDIS position statement regarding utilization of preimplantation genetic testing for aneuploidy (PGT-A) in association with in vitro fertilization (IVF). (biomedcentral.com)
- Huang J.C., Lei Z.L., Shi L.H., Miao Y.L., Yang J.W., Ouyang Y.C., Sun Q.Y., Chen D.Y., Comparison of histone modifications in in vivo and in vitro fertilization mouse embryos. (biologie-journal.org)
- The synchronization and ovulatory responses of Sangsari cross bred ewes and metabolism of energy substrates in 8-cell stage embryos to hatched blastocysts stage produced in vitro or in vivo were investigated. (biomedcentral.com)
- Surgical embryo recovery was performed on Days 4 and 6 after onset of estrus (Day 0) and recovered embryos were subjected to comparative metabolism studies with in vitro derived embryos at the same stage of development. (biomedcentral.com)
- While the pattern of oxidation was similar among in vitro and in vivo derived embryos, a low pyruvate to lactate ratio was the preferred substrate of embryos derived in vitro . (biomedcentral.com)
- The production of CO2 from glucose by sheep embryos has been determined[ 3 ] and results have indicated that little glucose is utilized by the embryos of sheep and that blastocysts derived from oocytes, matured and fertilized in vitro , oxidized glucose at a lower rate than those developed in vivo . (biomedcentral.com)
- Machine learning-assisted high-content analysis of pluripotent stem cell-derived embryos in vitro. (caltech.edu)
Bovine embryo2
- Kopecny V., Fléchon J.E., Camous S., Fulka J. Jr., Nucleologenesis , and the onset of transcription in the eight-cell bovine embryo: fine-structural autoradiographic study. (biologie-journal.org)
- Rieger[ 4 ] inferred that the pre-implantation bovine embryo undergoes an intensified period of cellular synthetic processes which require appropriate substrates for production of energy and reducing equivalents in the form of nicotinamide-adenine dinucleotide phosphate. (biomedcentral.com)
Embryogenesis6
- Demethylation during early embryogenesis occurs in the preimplantation period. (wikipedia.org)
- However, robust and large-scale genome-wide reprogramming of DNA methylome occurs during two critical developmental processes: (1) development of primordial germ cells and (2) pre-implantation embryogenesis. (nature.com)
- Although the genome-wide DNA demethylation is believed to be a hallmark of mammalian embryogenesis, previous study also indicated that the somatic form of dnmt1 ( dnmt1s ) is actually expressed at each stage of pre-implantation embryos and plays a role in the maintenance of DNA imprinting 8 . (nature.com)
- These results strongly suggest the possibility of DNA remethylation during pre-implantation embryogenesis. (nature.com)
- Integrins play important roles in several reproductive processes including fertilization, embryogenesis, and implantation. (go.jp)
- Promoters with altered H3K4me3 are enriched in genes involved in preimplantation and postimplantation embryogenesis, organ development, and chromatin remodeling. (molcells.org)
Blastocyst6
- After the blastocyst is formed, methylation can begin, and with formation of the epiblast a wave of methylation then takes place until the implantation stage of the embryo. (wikipedia.org)
- Methylation begins to increase at 3.5 days after fertilization in the blastocyst, and a large wave of methylation then occurs on days 4.5 to 5.5 in the epiblast, going from 12% to 62% methylation, and reaching maximum level after implantation in the uterus. (wikipedia.org)
- In mouse, a unidirectional demethylation process from the zygote stage to blastocyst stage is observed using either reduced representation bisulfite sequencing (RRBS) or single-base resolution whole-genome bisulfite sequencing (WGBS) method. (nature.com)
- Rossant, J. & Tam, P. P. L. Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse. (nature.com)
- Fleming, T. P. A quantitative analysis of cell allocation to trophectoderm and inner cell mass in the mouse blastocyst. (nature.com)
- As a treatment paradigm in routine IVF, PGT-A mandates cumulative add-ons with their own independent potential to adversely impact IVF outcomes, such as extended blastocyst culture, embryo cryopreservation, frozen embryo transfer and disposal of what the procedure reports as chromosomal-abnormal embryos. (biomedcentral.com)
Primordial germ2
- By day seven after fertilization, the newly formed primordial germ cells (PGC) in the implanted embryo segregate from the remaining somatic cells. (wikipedia.org)
- The newly formed primordial germ cells (PGC) in the implanted embryo devolve from the somatic cells. (wikipedia.org)
Post-implantation2
- Other than the PGCs, in the post-implantation stage, methylation patterns in somatic cells are stage- and tissue-specific with changes that presumably define each individual cell type and last stably over a long time. (wikipedia.org)
- A model of the post-implantation human embryo derived from pluripotent stem cells. (caltech.edu)
Cryopreservation1
- The maintenance of transgenes in rats is reviewed with special references to embryo cryopreservation (transgene banking) and intracytoplasmic injection of spermatozoa or round spermatids (transgene passage). (go.jp)
Pluripotent1
- Fulka H., St John J.C., Fulka J., Hozak P., Chromatin in early mammalian embryos: achieving the pluripotent state. (biologie-journal.org)
Undergoes1
- Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. (caltech.edu)
Sperm7
- The mouse sperm genome is 80-90% methylated at its CpG sites in DNA, amounting to about 20 million methylated sites. (wikipedia.org)
- After fertilization some cells of the newly formed embryo migrate to the germinal ridge and will eventually become the germ cells (sperm and oocytes) of the next generation. (wikipedia.org)
- Piotrowska, K. & Zernicka-Goetz, M. Role for sperm in spatial patterning of the early mouse embryo. (nature.com)
- Postnatal paternal folate deficiency increases congenital disabilities through H3K4me3 histone methylation changes in sperm and embryos. (molcells.org)
- Folate-deficient postnatal diet can change H3K4me3 patterns in sperm that are not erased after fertilization, but persist throughout preimplantation embryos. (molcells.org)
- Later, when these mice mature, they bred these male mice with WT female mice fed with a regular chow diet to examine pregnancy outcomes, H3K4me3 histone methylation in sperm and eight-cell embryos, and transcriptome of early embryos. (molcells.org)
- Paternal folate deficiency also worsens H3K4me3 alteration in TG sperm, and FD TG fathers' embryos show severer defects. (molcells.org)
Patterns1
- Graham, C. F. & Lehtonen, E. Formation and consequences of cell patterns in preimplantation mouse development. (nature.com)
Embryonic4
- Furthermore, due to ethical and legal concerns, very limited techniques can be applied to human embryos to validate some of significant conclusions drawn from descriptive studies regarding human embryonic development. (nature.com)
- Rossant, J. & Tam, P. P. L. Emerging asymmetry and embryonic patterning in early mouse development. (nature.com)
- While PGT-A has been proposed as a tool for achieving enhanced singleton livebirth outcomes through embryo selection, continued false-positive rates and increasing evidence for embryonic self-correction downstream from the testing stage, has led IDNHG-IVF to conclude that currently available data are insufficient to impose overreaching recommendations for PGT-A utilization. (biomedcentral.com)
- Modeling human embryo development with embryonic and extra-embryonic stem cells. (caltech.edu)
Zygotic1
- Bellier S., Chastant S., Adenot P., Vincent M., Renard J.P., Bensaude O., Nuclear translocation , and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos. (biologie-journal.org)
Demethylation1
- By the 8-cell stage, remethylation becomes more pronounced than demethylation, resulting in an increase in global DNA methylation. (nature.com)
Remethylation1
- Remethylation is needed again to differentiate the embryo into a complete organism. (wikipedia.org)
Fertilization2
- Adenot P.G., Szollosi M.S., Geze M., Renard J.P., Debey P., Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization. (biologie-journal.org)
- Haaf T., The battle of the sexes after fertilization: behaviour of paternal and maternal chromosomes in the early mammalian embryo. (biologie-journal.org)
Paternal4
- Adenot P.G., Mercier Y., Renard J.P., Thompson E.M., Differential H4 acetylation of paternal, maternal chromatin precedes DNA replication, and differential transcriptional activity in pronuclei of 1-cell mouse embryos. (biologie-journal.org)
- Howlett S.K., Reik W., Methylation levels of maternal , and paternal genomes during preimplantation development. (biologie-journal.org)
- These results show that developmental genes are highly vulnerable to postnatal paternal folate deficiency, reflecting congenital disabilities, such as abnormal eyes and skull bones, in early embryos born to FD WT fathers. (molcells.org)
- PCA of eight-cell embryo transcriptome revealed evident clustering based on paternal diet and genotype. (molcells.org)
Chromatin1
- Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W., Godke R.A., Bondioli K.R., Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. (biologie-journal.org)
Methylation3
- The morula (at the 16 cell stage), has only a small amount of DNA methylation (black line in Figure). (wikipedia.org)
- Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. (nature.com)
- Arney K.L., Bao S., Bannister A.J., Kouzarides T., Surani M.A., Histone methylation defines epigenetic asymmetry in the mouse zygote. (biologie-journal.org)
Transgenic2
- Two-cell stage embryos derived from the mating of transgenic rats with wild-type rats can be successfully cryopreserved by conventional two-step freezing in the presence of 10% dimethylsulfoxide. (go.jp)
- fed wild-type (WT) or KDM1A histone demethylase transgenic (TG) male mice with folate-sufficient (FS) or folate-deficient (FD) diet after weaning, establishing four experimental groups: FS WT, FD WT, FS TG, and FD TG. (molcells.org)
Morula2
- Johnson, M. H. & Ziomek, C. A. The foundation of two distinct cell lineages within the mouse morula. (nature.com)
- The first marked increase in the metabolism of glucose by ovine embryos was detected in compact morula stage, but there was no significant increase in the oxidation of glucose after the morula stage. (biomedcentral.com)
Synthetic1
- Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension. (caltech.edu)
Development3
- Unlike in rodents, X chromosome inactivation is not observed during monkey pre-implantation development. (nature.com)
- The mechanisms underlying the appearance of asymmetry between cells in the early embryo and consequently the specification of distinct cell lineages during mammalian development remain elusive. (nature.com)
- Braude P., Bolton V., Moore S., Human gene expression first occurs between the four- , and eight-cell stages of preimplantation development. (biologie-journal.org)
Pregnancy1
- The FD diet increases variability in pregnancy loss at both preimplantation and postimplantation stages. (molcells.org)
Vivo1
- Rossant, J. & Lis, W. T. Potential of isolated mouse inner cell masses to form trophectoderm derivatives in vivo . (nature.com)
Histone1
- To determine whether dysregulation of TGFβ/Klf11/Cyp3a signaling affected endometriotic progression, we treated wild-type control and Klf11-/- mice with a Tgfβ type 1 receptor inhibitor (TGFβR1I) that inhibits Tgfβ signaling upstream of the canonical Smad proteins or a combination of TGFβR1I and a histone acetyltransferase inhibitor that additionally inhibits Klf11 signaling. (endometriosi.it)
Outcomes1
- Mindful of what appears to offer best outcomes for patients, and in full consideration of patient autonomy, here presented opinion is based on best available evidence, with the goal of improving safety and efficacy of IVF and minimizing wastage of embryos with potential for healthy births. (biomedcentral.com)
Trophectoderm1
- Human embryo polarization requires PLC signaling to mediate trophectoderm specification. (caltech.edu)
Stem2
- Generation of Stem Cell-Based Mouse Embryo-Like Structures. (caltech.edu)
- Stem-cell-based human and mouse embryo models. (caltech.edu)
Cells2
- Rossant, J. & Vijh, K. M. Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. (nature.com)
- Endometriosis, a chronic disease of heterogeneous etiopathology affects 10% of young women and is characterized by ectopic implantation of endometrial cells. (endometriosi.it)
Occurs1
- Bouniol C., Nguyen E., Debey P., Endogenous transcription occurs at the 1-cell stage in the mouse embryo. (biologie-journal.org)
Early mouse1
- Figure 1: Classic models for lineage segregation in the early mouse embryo. (nature.com)
Transfer1
- Synchronization of estrus followed by induction of ovulation are basic techniques in all embryo transfer programs. (biomedcentral.com)
Activity1
- Aoki F., Worrad D.M., Schultz R.M., Regulation of transcriptional activity during the first , and second cell cycles in the preimplantation mouse embryo. (biologie-journal.org)
Male1
- For example, male mice fed with a low-folate diet produce offsprings showing a spectrum of congenital skeletal malformations. (molcells.org)
Position1
- Driever, W. & Nüsslein-Volhard, C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. (nature.com)
Increase1
- This downward trend over the past decade has paralleled a marked increase in the use of preimplantation genetic testing (PGT-A) and of other so-called add-ons to IVF. (biomedcentral.com)
Days1
- Reprogrammings that are both large scale (10% to 100% of epigenetic marks) and rapid (hours to a few days) occur at three life stages of mammals. (wikipedia.org)
Energy1
- Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. (nature.com)