• However, the control of directing pluripotent cells into mesoderm lineage which gives rise to cardiac muscle cells remains poor. (siu.edu)
  • This is a proof-of-concept that mechanical signaling together with synergistic chemical signaling can drive pluripotent cells towards mesoderm lineage. (siu.edu)
  • Future studies with human pluripotent stem cells may transduce cells towards mesoderm and finally towards cardiac fate. (siu.edu)
  • Transduction of Lhx2 into induced pluripotent stem cells (iPSCs) was also effective in generating KSL cells in vitro, as well as HSC-like activities in vivo. (ebiotrade.com)
  • Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (razavihospital.ir)
  • We have used RT-PCR to screen pluripotent murine embryonic stem cells to identify receptor tyrosine kinases (RTKs) potentially involved in the determination or differentiation of cell lineages during early mouse development. (silverchair.com)
  • In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. (biomedcentral.com)
  • According to their potentiality, embryonic stem cells (ESCs) are classified as totipotent and pluripotent, both of them can differentiate into the three germ lines. (biomedcentral.com)
  • Embryonic stem cells or induced pluripotent stem cells (iPSCs) are also utilized for AKI recovery. (biomedcentral.com)
  • Induced pluripotent stem cells (iPSCs) are a type of newly defined stem cell with properties similar to those of ESCs in terms of self-renewal and differentiation. (biomedcentral.com)
  • Chemical conversion of human conventional Pluripotent Stem Cells to Trophoblast Stem Cells. (axonmedchem.com)
  • Revealing cell populations catching the early stages of the human embryo development in naïve pluripotent stem cells. (axonmedchem.com)
  • a naïve LIF-dependent pluripotent state which is represented by murine embryonic stem cells (mESCs) and an FGF-dependent primed pluripotent state represented by murine and rat epiblast stem cells (EpiSCs). (mdc-berlin.de)
  • We find that derivation of induced pluripotent stem cells (iPSCs) under EpiSC culture conditions yields FGF-dependent iPSCs from hereon called FGF-iPSCs) which, unexpectedly, display naïve ES-like/ICM properties. (mdc-berlin.de)
  • Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions. (mdc-berlin.de)
  • Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
  • Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
  • However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
  • This study systematically analyzes multiple-omics data and builds a systems physical network including genome-wide interactions between miRNAs and their targets to reveal the systems mechanism of miRNA functions in mouse pluripotent stem cells. (biomedcentral.com)
  • Globally, miRNAs directly repress the pluripotent core factors during differentiation state. (biomedcentral.com)
  • While miRNAs directly repress pluripotent core factors to facilitate differentiation during the differentiation state, they also help stem cells to maintain pluripotency by activating pluripotent cores through directly repressing DNA methylation systems and primarily inhibiting development in the pluripotent state. (biomedcentral.com)
  • This present study employed systems physical network approaches [ 17 ] and constructed a comprehensive and unbiased map of genome-wide interactions between miRNAs and their targets to investigate the global basis of miRNA roles in pluripotent stem cells, from the pluripotent self-renewal state to the differentiation state. (biomedcentral.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
  • It appears that at these early stages of development, STAT3 activation is required for self-renewal of embryonic stem cells (ESCs). (wikipedia.org)
  • Lineage commitment during Embryonic Stem Cells (ESCs) differentiation is controlled not only by a gamut of transcription factors but also by epigenetic events, mainly histone deacetylation and promoter DNA methylation. (refine.bio)
  • Thus, the purpose of this study was to examine the effects of zebularine, a stable and non-toxic DNA cytosine methylation inhibitor, on ESCs differentiation. (refine.bio)
  • Herein are the Affymetrix Expression data obtained from RNA of murine ESCs treated with zebularine. (refine.bio)
  • Identification of genes involved in in vitro differentiation induction of embryonic stem cells (ESCs) into hematopoietic stem cells (HSCs) has been challenged during last decade. (ebiotrade.com)
  • Here, we show that HSC-like cells were efficiently induced from mouse ESCs by enforced expression of Lhx2, a LIM-homeobox transcription factor. (ebiotrade.com)
  • ESCs are obtained from inner cell mass, characterized by unlimited self-renewal and pluripotentiality, which allow them to differentiate into various specialized cells types in a morphologically and functionally way, characteristics that make them attractive for developing differentiated kidney cells (DKCs) [ 9 ]. (biomedcentral.com)
  • Stem cells, including embryonic stem cells (ESCs) and adult stem cells, have the potential of self-renewal and differentiation into multiple cell types and therefore hold great promise in regenerative medicine [ 11 - 13 ]. (biomedcentral.com)
  • 10 ] showed that the transplantation of mouse ESCs-derived RPCs can result in the stable integration into proximal tubules with normal morphology and normal polarization injection into developing live newborn mouse kidneys, suggesting the potential of ESCs for application in regenerative therapies. (biomedcentral.com)
  • moreover, they also promote the hematopoietic differentiation of mouse ESCs and favor the development of definitive HSCs from pre-HSCs in the mouse aorta-gonad-mesonephros (AGM) region [ 8 ]. (biomedcentral.com)
  • Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
  • Furthermore, transplantation of Lhx2-transduced hematopoietic cells into lethally irradiated mice resulted in multi-lineage repopulation of hematopoietic cells over 4 months. (ebiotrade.com)
  • These results demonstrate that ectopic expression of Lhx2 confers an in vivo engrafting capacity to ESC/iPSC-derived hematopoietic cells and in vivo behavior of iPSC-derived hematopoietic cells is almost identical to that of ESC-derived cells. (ebiotrade.com)
  • Several HOX transcription factors, including HOXB4, are highly expressed in hematopoietic stem cells (HSCs) but are downregulated upon differentiation towards more mature progeny cells. (dissertations.se)
  • Adenoviral mediated overexpression of HOXB4 in human primitive hematopoietic cells in vitro generates very high levels of HOXB4 within 24 hours of transduction, leading to an increased differentiation towards myeloid lineages, and to a reduction of primitive hematopoietic progenitors, suggesting that the level of HOXB4 expression may determine the fate options following gene transfer. (dissertations.se)
  • Studies in our knockout mice reveal that deficiency of HOXB4 leads to a hypocellularity in hematopoietic organs and impaired proliferative capacity. (dissertations.se)
  • SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. (fgf-4.com)
  • In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
  • The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
  • Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
  • We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
  • Moreover, CD201 + hematopoietic cells remained in a relatively slow cycling state, consistent with high expression levels of P57 and Ccng2. (biomedcentral.com)
  • Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
  • Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
  • In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)
  • Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
  • Mathematical modeling of Hematopoietic Stem Cell and Leukaemic Stem Cell culture systems. (imperial.ac.uk)
  • The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
  • Comparison of toxicity of benzene metabolite hydroquinone in hematopoietic stem cells derived from murine embryonic yolk sac and adult bone marrow. (cdc.gov)
  • It is believed that benzene and its metabolites target hematopoietic stem cells (HSCs) to cause toxicity and cancer in the hematopoietic system. (cdc.gov)
  • In the current study, we compared the effects of hydroquinone (HQ), a major metabolite of benzene in humans and animals, on mouse embryonic yolk sac hematopoietic stem cells (YS-HSCs) and adult bone marrow hematopoietic stem cells (BM-HSCs). (cdc.gov)
  • The study established an experimental system for comparison of the hematopoietic toxicity and leukemogenicity of benzene and metabolites during mouse embryonic development and adulthood. (cdc.gov)
  • We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
  • Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. (biomedcentral.com)
  • Embryonic or adult SCs by definition are characterized by their self-renewal and potentiality, peculiarities that allow them to give rise to more SCs and differentiate into various cell lineages under appropriate conditions. (biomedcentral.com)
  • In this group of CGIs, repressive polycomb group (PcG) complexes act to block transcription in inappropriate lineages or at non-expressing stages during the differentiation programme. (biomedcentral.com)
  • At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
  • Thoma et al observed that Ngn2 alone is sufficient to induce neuronal differentiation in embryonic stem cells. (bartleby.com)
  • Altering FAs by other means did not induce the same changes in pAkt as those seen by reducing ARAP2 in U118 cells. (bio2009.org)
  • On the other hand, miRNAs like let-7 induce stem cell differentiation [ 11 ]. (biomedcentral.com)
  • Membrane vesicles, actively released by cells, represent a mechanism of intercellular communication that is conserved evolutionarily and involves the transfer of molecules able to induce epigenetic changes in recipient cells. (unicyte.ch)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
  • Shih CC, Forman SJ, Chu P, Slovak M. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. (razavihospital.ir)
  • Promotes maturation of fetal mouse hepatocytes (Kamiya et al. (stemcell.com)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • FGF-iPSCs display X-chromosome activation, multi-lineage differentiation, teratoma competence and chimera contribution in vivo. (mdc-berlin.de)
  • In fact, we show here that aged murine MDSCs (MDSCs) exhibit reduced proliferation and multi-lineage differentiation potential with or without BMP4 stimulation in vitro, when compared to young murine MDSCs. (pitt.edu)
  • Targeted inactivation of p12, CDK2 associating protein 1, leads to early embryonic lethality. (nih.gov)
  • HQ reduced the proliferation and the differentiation and colony formation, but increased the apoptosis of both YS-HSCs and BM-HSCs. (cdc.gov)
  • The results revealed differential effects of benzene metabolites on embryonic and adult HSCs. (cdc.gov)
  • Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. (razavihospital.ir)
  • Kurosawa H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. (razavihospital.ir)
  • After finding favorable overlap between antibody labeling and the endogenous fluorescent signal of Eln, we used this endogenous signal to map temporal changes in Eln and ColI during murine embryoid body differentiation and found that Eln increases until day 9 and then decreases slightly by day 12, while Col1 steadily increases over the 12-day period. (umn.edu)
  • Expression of cell-cycle regulator CDK2-associating protein 1 (p12CDK2AP1) in transgenic mice induces testicular and ovarian atrophy in vivo. (nih.gov)
  • At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
  • On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
  • The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
  • These results taken together suggest that BMP4 regulates the cell cycle of aged MDSCs and consequently promotes their osteogenic potential in vitro and in vivo. (pitt.edu)
  • Here we identify extensive transcriptional changes that occur during murine astrocyte maturation in vivo that are accompanied by chromatin remodelling at enhancer elements. (nature.com)
  • The discovery of murine norovirus (MNV) in 2003 and the fact that this virus successfully replicated in a murine macrophage cell line in vitro and in primary immune cells in vivo suggested that immune cells may also support replication of human norovirus ( 7 ). (cdc.gov)
  • The HIEs, or "mini-guts," are generated from stem cells present in the intestinal crypts isolated from human intestinal tissue and cultured indefinitely as ex vivo, 3-dimensional (3D) cultures in growth-factor-enriched media ( 13 - 15 ). (cdc.gov)
  • Acts upstream of or within face morphogenesis and in utero embryonic development. (nih.gov)
  • A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform essential for development. (nih.gov)
  • Whereas vitamin A is best known for its critical role during embryonic development and visual cycle (documented by 3 Nobel prizes), its post-natal activities, including in neuroprotection, modulation of neurotransmission and synaptic plasticity only start being discovered and understood. (igbmc.fr)
  • Our goal is to decipher the highly diverse functions of vitamin A, its active derivatives (retinoic acids), and the corresponding nuclear receptors (RARs/RXRs), in neural development, aging, and in stem cell control. (igbmc.fr)
  • Our studies of specific retinoid receptors and bioactive metabolites in control of diverse biological processes should contribute to a better understanding of the mechanisms underlying development and function of specific cell types and relevant neural circuits. (igbmc.fr)
  • We also investigate mechanisms of glial cell development and biology to elaborate regenerative medicine approaches in multiple sclerosis . (igbmc.fr)
  • Moscona A. The Development in Vitro of Chimeric Aggregates of Dissociated Embryonic Chick and Mouse Cells. (razavihospital.ir)
  • This work provides an important first step for utilizing endogenous optical signals, which can be visualized in living cells, to understand the relationship between the ECM and cardiomyocyte development and sets the stage for future studies of stem cell-ECM interactions and dynamics relevant to stem cells as well as other cell and tissue types. (umn.edu)
  • All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
  • HOXB4 is one of 39 members of the homeobox (HOX) family, a family of transcription factors involved in embryonic development. (dissertations.se)
  • The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. (biomedcentral.com)
  • Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. (biomedcentral.com)
  • The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
  • Embryonic development is tightly controlled at the level of gene expression. (amegroups.org)
  • MiRNAs critically modulate many cellular events, including the balance between proliferation and differentiation during organ development [ 1 ]. (biomedcentral.com)
  • Astrocytes are the most abundant glial cells in the mammalian central nervous system and they serve essential functions in brain development and homoeostasis. (nature.com)
  • A tight spatial and timing regulation of growth factor action during embryonic development has been suggested [4]. (unicyte.ch)
  • The data indicate that scFv78, the first fully human anti-TEM1 recombinant antibody, recognizes both human and mouse TEM1 and has unique and favorable features that are advantageous for the development of imaging probes or antibody-toxin conjugates for a large spectrum of human TEM1-positive solid tumors. (atsbio.com)
  • Transcription factors (nurr1, pitx3 and lmx1a) that promote embryonic mouse or chicken dopaminergic development were employed to determine if they would modulate lineage restriction of these adult human progenitors. (scirp.org)
  • A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. (thermofisher.com)
  • TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. (thermofisher.com)
  • The first steps toward a new cell culture system for human norovirus included the detection of human norovirus antigen in duodenal and jejunal enterocytes in tissue sections from human norovirus-infected transplant patients ( 11 ) and the development of human intestinal enteroids (HIEs) derived from nontransformed small intestine and colonic tissues ( 12 ). (cdc.gov)
  • Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
  • However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
  • STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. (wikipedia.org)
  • Increased activity of STAT3 in cancer cells, leads to changes in the function of protein complexes that control expression of inflammatory genes, with result profound change in the secretome and the cell phenotypes, their activity in the tumor, and their capacity for metastasis. (wikipedia.org)
  • Genes Cells. (razavihospital.ir)
  • The transcripts of most expressed genes, in a variety of cell types, were shown to be methylated, indicating that m6A modifications are widespread. (pharmaceuticalintelligence.com)
  • The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
  • However, these recent studies have mostly focused on individual gene functions in stem cells although genome-wide data might be employed, and the conclusions drawn from these current studies are unavoidably biased on genes selected by these studies. (biomedcentral.com)
  • Investigating astrocyte maturation in a cell culture model revealed that in vitro-differentiated astrocytes lack expression of many mature astrocyte-specific genes, including genes for the transcription factors Rorb, Dbx2, Lhx2 and Fezf2. (nature.com)
  • Mouse α-globin genes, which lack an annotated CGI, exhibit tightly regulated tissue and developmental stage-specific regulation similar to that observed for the human α-globin genes, which contain a CGI and are regulated by PcG [ 9 ]. (biomedcentral.com)
  • explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
  • Transgenic reporter mouse embryonic stem cells were used in this study. (siu.edu)
  • 2012 ) Transgenic enrichment of mouse embryonic stem cell-derived progenitor motor neurons. (neurotree.org)
  • The results reveal that the loss of BFCNs in pre-symptomatic pR5 tau transgenic mice results in a decrease in hippocampal brain-derived neurotrophic factor levels and reduced TrkB receptor activation. (atsbio.com)
  • 7. Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice. (whocc.org.cn)
  • To promote adhesion of mouse embryonic stem cells and to initiate integrin-based mechanical signaling, the extracellular matrix protein fibronectin was used. (siu.edu)
  • Czyz J, Wobus A. Embryonic stem cell differentiation: the role of extracellular factors. (razavihospital.ir)
  • Components of the extracellular matrix (ECM) have recently been shown to influence stem cell specification. (umn.edu)
  • FAs are structures composed of clustered transmembrane proteins called integrins that bind to the extracellular matrix and link to the actin cytoskeleton and control cell migration, proliferation, survival and differentiation (Gardel et al. (bio2009.org)
  • Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming. (unicyte.ch)
  • In the present review we focus on the extracellular vesicle-induced epigenetic changes in recipient cells that may lead to phenotypic and functional modifications. (unicyte.ch)
  • In particular, stem cells are highly sensitive to extracellular signals that play a critical role in mainten- ance of stem cell characteristics, differentiation, and interplay with somatic cells. (unicyte.ch)
  • Similarly, reprogramming of somatic cells involves a complex interaction among intracellular and extracellular signals leading to epigenetic remodeling [6]. (unicyte.ch)
  • Promotes osteogenic, adipogenic, and chondrogenic differentiation of human mesenchymal cells (Jaiswal et al. (stemcell.com)
  • Promotes osteogenic, adipogenic, and chondrogenic differentiation of mouse mesenchymal cells (Tropel et al. (stemcell.com)
  • Cell-based therapy with mesenchymal stem cells or renal progenitor cells (RPCs) resident in the kidney has appeared to be an effective strategy for the treatment of AKI. (biomedcentral.com)
  • Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. (nih.gov)
  • The reporter cell line shows the cellular endogenous activity of pluripotency gene Oct3/4 with green fluorescent protein (GFP) while also reporting mesoderm-specific gene, Brachyury, activity with DsRed fluorescent protein. (siu.edu)
  • To further promote differentiation and to increase mechanical signaling, Notch signaling was activated by presenting cells with fibronectin and DLL1 protein. (siu.edu)
  • The differentiation was found to have a pronounced effect in the presence of fibronectin and DLL1 protein together with the withdrawal of LIF. (siu.edu)
  • Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. (fgf-4.com)
  • Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds. (fgf-4.com)
  • Recombinant Murine EGF is a 6.0 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds. (fgf-4.com)
  • Recombinant Murine TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein. (fgf-4.com)
  • GCNA is a histone binding protein required for spermatogonial stem cell maintenance. (axonmedchem.com)
  • Bone morphogenetic protein 4 (BMP4) promotes the osteogenic potential & the bone regenerative potential of muscle-derived stem cells (MDSCs). (pitt.edu)
  • Enforced expression of HOXB4 dramatically increases the regeneration of murine HSC following transplantation, and enhances the repopulating ability of human SCID repopulating cells. (dissertations.se)
  • The transplantation of iPSC-derived RPCs decreased the levels of biomarkers indicative of renal injury and attenuated the necrosis and apoptosis of renal tissues, but resulted in the up-regulation of renal tubules formation, cell proliferation, and the expression of pro-renal factors. (biomedcentral.com)
  • Repair and mechanisms of MEECs transplantation in IUA mice were measured with qRT-PCR, Masson's trichrome, and DAB staining. (biomedcentral.com)
  • Most importantly, allogeneic transplantation of CR-MEECs successfully repaired the injured endometrium and significantly improved the pregnancy rate of IUA mice. (biomedcentral.com)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • Fundamental questions in organogenesis are related to the identification of a simplified model which is able to mimic the molecular mechanisms involved in pattern organization and cell fate determination as well. (razavihospital.ir)
  • Molecular cues for pluripotency, genetic reprogramming of adult stem cells, and widely multipotent adult cells. (razavihospital.ir)
  • Thus, GATA-2 and GATA-3 regulate adipocyte differentiation through molecular control of the preadipocyte-adipocyte transition. (nih.gov)
  • Molecular Cell , 82 (1), 190-208. (axonmedchem.com)
  • Molecular cell, 82(1), 106-122. (axonmedchem.com)
  • Characterization of the key molecular signalling pathways revealed FGF-iPSCs to depend on the Activin/Nodal and FGF pathways, while signalling through the JAK-STAT pathway is not required for FGF-iPS cell maintenance. (mdc-berlin.de)
  • I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
  • I cover mainly cell biology and physiology. (nottingham.ac.uk)
  • Conditionally reprogrammed physiological endometrial epithelial cells provide a novel strategy in IUA clinics in a personalized or generalized manner and also serve as a physiological model to explore biology of endometrial epithelial cells and mechanisms of IUA. (biomedcentral.com)
  • The relevance of these phenomena in stem cell biology and tissue repair is discussed. (unicyte.ch)
  • Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
  • To address these questions, we use genetic, pharmacological or behavioral mouse models of specific diseases, as well as CRISPR- and viral-based approaches to control gene expression. (igbmc.fr)
  • This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)
  • In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
  • This complexity of miRNA interaction network presents a challenge for conventional approaches like gene-knockout to unbiasedly capture the real mechanisms of miRNA functions in stem cells. (biomedcentral.com)
  • 2008 ) Embryonic stem cells as a platform for analyzing neural gene transcription. (neurotree.org)
  • 2007 ) Polony analysis of gene expression in ES cells and blastocysts. (neurotree.org)
  • Gene networks reveal stem-cell state convergence during preneoplasia and progression to malignancy in multistage skin carcinogenesis. (ucsf.edu)
  • Potentiality of embryonic stem cells: an ethical problem even with alternative stem cell sources. (razavihospital.ir)
  • Ectopic expression of Ngn2 was sufficient to form mature neurons from embryonic stem cells[5]. (bartleby.com)
  • This study optimized conditions for the differentiation of hONPs towards dopaminergic neurons. (scirp.org)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • They also showed a marked reduction in the number of neural stem progenitors in DG. (bartleby.com)
  • The use of olfactory progenitors as a cell-based therapy for Parkinson's disease (PD) would allow harvest without invasive surgery, provide an autologous cell population, eliminate need for immunosuppression and avoid the ethical concerns associated with embryonic tissues. (scirp.org)
  • It has been reported that BMP promotes the self-renewal of both embryonic and somatic stem cells and that BMP signaling activity significantly decreases with age, thus we posit that BMP induction may improve the function of MDSCs. (pitt.edu)
  • Furthermore, we combined endogenous fluorescence imaging and SHG with antibody labeling of cardiomyocytes to examine the spatial relationship between Eln and ColI accumulation and cardiomyocyte differentiation. (umn.edu)
  • Human and murine TPO exhibit cross-species reactivity. (fgf-4.com)
  • GATA-3-deficient embryonic stem cells exhibit an enhanced capacity to differentiate into adipocytes, and defective GATA-2 and GATA-3 expression is associated with obesity. (nih.gov)
  • Ngns (class II bHLH transcription factors) are known as pro-neural factors as they are necessary to initiate differentiation of NSPCs and are important to specify a neuronal subtype[3]. (bartleby.com)
  • In this study they used murine embryonic stem cells and transfected with Ngn2 expression and after five days post transfection they observed that cells expressed Tuj1 and MAP2 (both are neuronal markers). (bartleby.com)
  • In response to cytokines and growth factors, STAT3 is phosphorylated by receptor-associated Janus kinases (JAK), forms homo- or heterodimers, and translocates to the cell nucleus where it acts as a transcription activator. (wikipedia.org)
  • While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. (fgf-4.com)
  • Cell-to-cell signalling is induced by specific ligands such as growth factors which activate their cognate receptor molecules. (amegroups.org)
  • 2020 ) Chimeric antigen receptor T-cell therapy after allogeneic stem cell transplant for relapsed/refractory large B-cell lymphoma. (neurotree.org)
  • Eln was ubiquitously present, with enrichment in regions with cardiomyocyte differentiation, while there was an inverse correlation between ColI and cardiomyocyte differentiation. (umn.edu)
  • 1. Corn silk induces nitric oxide synthase in murine macrophages. (whocc.org.cn)
  • In this study, we induced iPSCs differentiation into RPCs using a nephrogenic cocktail of factors combined with the renal epithelial cell growth medium. (biomedcentral.com)
  • We showed that the differentiation efficiency of iPSCs to RPCs increased when cultured with renal epithelial cell growth medium after stimulation with a nephrogenic cocktail of factors. (biomedcentral.com)
  • The expression patterns of four RTKs have been examined and all are expressed in the mouse embryo during, or shortly after, gastrulation. (silverchair.com)
  • Constitutive GATA-2 and GATA-3 expression suppressed adipocyte differentiation and trapped cells at the preadipocyte stage. (nih.gov)
  • We hypothesized that BMP4 might exert beneficial effects through modulation of p16 and p18 expression in transduced MDSCs since the cyclin-dependent kinase inhibitors p16INK4A (p16) and p18INK4C (p18) cause early G1-phase cell cycle blockade. (pitt.edu)
  • Lorberbaum DS, Gottlieb D. ( 2011 ) Regulated expression of transgenes in embryonic stem cell-derived neural cells. (neurotree.org)
  • Transcription factor expression and the rate-limiting enzyme in dopamine synthesis tyrosine hydroxylase (TH) were detected in the transfected cells after 4 month-selection with G418, indicating transfected hONPs were stably restricted towards a dopaminergic lineage. (scirp.org)
  • Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R. (thermofisher.com)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
  • Our results revealed that iPSC-derived RPCs can protect AKI rat from renal function impairment and severe tubular injury by up-regulating the renal tubules formation, promoting cell proliferation, reducing apoptosis, and regulating the microenvironment in the injured kidney. (biomedcentral.com)
  • The cell phenotype is therefore determined by signals that target the cells received within a defined microenvironment. (unicyte.ch)
  • 13. Pseudolaric acid B induces apoptosis via activation of c-Jun N-terminal kinase and caspase-3 in HeLa cells. (whocc.org.cn)
  • Indeed, LIF, which is supplied to murine ESC cultures to maintain their undifferentiated state, can be omitted if STAT3 is activated through some other means. (wikipedia.org)
  • Mouse endometrial epithelial cells (MEECs) were isolated from C57BL female mice, and long-term cultures of MEECs were established and maintained with conditional reprogramming (CR) method. (biomedcentral.com)
  • HIE cultures recapitulate the complexity and cell diversity of the gastrointestinal tract in relatively the same proportions as in the intestine itself ( 14 , 15 ) and successfully support the replication of human rotavirus ( 13 ) and human norovirus ( 16 , 17 ). (cdc.gov)
  • STAT3-deficient mouse embryos cannot develop beyond embryonic day 7, when gastrulation begins. (wikipedia.org)
  • Moscona A. Cell suspensions from organ rudiments of chick embryos. (razavihospital.ir)
  • Efficient generation of embryonic stem cells from single blastomeres of cryopreserved mouse embryos in the presence of signalling modulators. (axonmedchem.com)
  • In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
  • First, we are interested in Epstein Barr Virus-mediated mechanisms of immune evasion with particular emphasis on pathways that promote survival and proliferation of EBV B cell lymphomas, the characterization of the human T cell and NK cell response to EBV and the identification of novel therapeutics for treatment of EBV B cell lymphomas. (stanford.edu)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
  • Ying QL, Stavridis M, Griffiths D, Li M, Smith A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent Ferro F et al. (razavihospital.ir)
  • Here, we show that murine GATA-2 and GATA-3 are specifically expressed in white adipocyte precursors and that their down-regulation sets the stage for terminal differentiation. (nih.gov)
  • Anderson, L. and Caldwell, M.A. (2007) Human neural progenitor cell transplants into the subthalamic nucleus lead to functional recovery in a rat model of Parkinson's disease. (scirp.org)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel. (razavihospital.ir)
  • Promotes differentiation of mature hepatocytes from mouse and human embryonic stem (ES) cells (Cai et al. (stemcell.com)
  • The Animal Serum Locor reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (fgf-4.com)
  • Stability of Imprinting and Differentiation Capacity in Naïve Human Cells Induced by Chemical Inhibition of CDK8 and CDK19. (axonmedchem.com)
  • 2019 ) Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding. (neurotree.org)
  • Here, we used ChIP followed by high-throughput sequencing (ChIP-seq) to analyse genomic occupancy of CFP1 in two human haematopoietic cell types. (biomedcentral.com)
  • Usage: Mouse endothelial cells (MS1) and MS1 cells transduced to express full-length human TEM1 (MS1-TEM1) were cultured in 96-well plates to 30% confluence and then incubated for 96h in the presence of 10-fold serially diluted Streptavidin-ZAP, scFv78, or scFv78-ZAP starting from 40nM down to 0.04nM. (atsbio.com)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • Immunohisto- have been used for positive selection of NSCs from em- chemistry on human embryonic central nervous system bryonic mice (Nagato et al. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • Initial studies included a comprehensive number of primary and continuous cell lines that had been used successfully to grow other viruses, but none supported human norovirus replication ( 2 ). (cdc.gov)
  • Other attempts included the use of a differentiated human embryonic small-intestinal cell line (INT 407) ( 3 ), but the results could not be confirmed by others ( 4 - 6 ). (cdc.gov)
  • A more recent study reported that BJAB cells, a continuous human B cell line, supported replication of a GII.4 norovirus strain in the presence of bacteria ( 9 ). (cdc.gov)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
  • Komura H, Ogita H, Ikeda W, Mizoguchi A, Miyoshi J, Takai Y. Establishment of cell polarity by afadin during the formation of embryoid bodies. (razavihospital.ir)
  • 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
  • MicroRNAs (miRNAs) critically modulate stem cell properties like pluripotency, but the fundamental mechanism remains largely unknown. (biomedcentral.com)
  • Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
  • Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
  • Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process. (biomedcentral.com)
  • To gain insights into the mechanisms driving astrocyte maturation, we have characterised acutely isolated murine astrocytes at both postnatal and adult stages, as well as in vitro differentiated astrocytes. (nature.com)
  • Promotes transdifferentiation of hepatocytes from mouse pancreatic cells (Shen et al. (stemcell.com)
  • LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. (fgf-4.com)
  • To date, a homeobox transcription factor Hoxb4 has been only demonstrated to possess such an effect in mice. (ebiotrade.com)
  • We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)
  • Applications Tested: This Dan11mag antibody has been tested by intracellular flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. (thermofisher.com)
  • Enhanced TGF-� Signaling Contributes to the Insulin-Induced Angiogenic Responses of Endothelial Cells. (ucsf.edu)
  • SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity. (axonmedchem.com)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • The stage-specific embryonic antigen 4 (SSEA4) is brain. (lu.se)
  • Using a model of unpredictable maternal separation and maternal stress (MSUS) in mice 13 , we examined whether trauma exposure in postnatal life can have beneficial effects on behaviour in the offspring. (nature.com)
  • We used MSUS 13 to examine whether traumatic stress in postnatal life can have behavioural benefits in the offspring of mice. (nature.com)
  • Neurogenesis occurs in the embryonic stage and astrogliogenesis in the postnatal stage. (bartleby.com)
  • During a subsequent phase of maturation in the first few postnatal weeks, immature astrocytes exit the cell cycle and aquire a fully mature phenotype 1 . (nature.com)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • The KSL cell induction frequency was superior to the case of Hoxb4. (ebiotrade.com)
  • The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
  • We also showed that aged MDSCs retrovirally transduced to express BMP4 can regenerate bone in a critical-sized skull defect in 6-week-old CD-1 nude mice, but the bone regenerative potential is inferior to young BMP4 expressing MDSCs. (pitt.edu)
  • During viral infection, mice lacking STAT3 in T-cells display impairment in the ability to generate T-follicular helper (Tfh) cells and fail to maintain antibody based immunity. (wikipedia.org)
  • A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
  • α-PD-1 therapy elevates Treg/Th balance and increases tumor cell pSmad3 that are both targeted by α-TGFβ antibody to promote durable rejection and immunity in squamous cell carcinomas. (ucsf.edu)
  • Transduction of Lhx2 into ESC-derived mesodermal cells resulted in robust differentiation of c-Kit+/Sca-1+/Lineage- (KSL) cells in vitro. (ebiotrade.com)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
  • 2007 ) The effects of soluble growth factors on embryonic stem cell differentiation inside of fibrin scaffolds. (neurotree.org)
  • Cells can communicate via physical interactions, in- cluding membrane bridge formation, such as tunneling nanotubes and cytonemes, and/or through the release of soluble factors [1-3]. (unicyte.ch)