Mouse embryonic stem cells mice cell cycle. Medical search. Frequent questions

Single-cell RNA sequencing studies on gene co-expression patterns could yield important regulatory and functional insights, but have so far been limited by the confounding effects of differentiation and cell cycle. (nih.gov)
We discovered that activation of BMP signaling promotes the exit of DIPG tumor cells from 'prolonged stem-cell-like' state to differentiation by epigenetically regulating CXXC5, which acts as a tumor suppressor and positive regulator of BMP signaling. (nature.com)
Thus, targeting the prolonged stem-cell-like state of DIPG cells to differentiation has been proposed as a potential strategy for DIPG treatment. (nature.com)
Mouse studies have suggested that Acvr1 G328V cooperates with Hist1h3b K27M and Pik3ca H1047R to generate high-grade diffuse gliomas, specifically by arresting the differentiation of oligodendroglial lineage cells 16 . (nature.com)
Furthermore, we used this assay to determine the effects of BRCA1 variants on cell cycle regulation, differentiation, and genomic stability. (jci.org)
To establish protocols for the safe and efficient differentiation of healthy cells for therapies, we must develop a better understanding of the dynamic continuum of metabolic states that span pluripotency and differentiation, and how to influence them. (conditionmed.org)
Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
The models use isolated primary support cells cultured onto a biological scaffold and within a microfluidic system to create a niche and support the desired differentiation of epithelia, germ and somatic cells from patient-derived induced pluripotent stem cells. (biomedcentral.com)
Morphological changes during cell differentiation are linked to IF network remodeling. (biomedcentral.com)
However, little is known concerning the presence and the role of IFs in embryonic stem (ES) cells and during their differentiation. (biomedcentral.com)
We have examined the expression profile of synemin isoforms in mouse pluripotent ES cells and during their neural differentiation induced by retinoic acid. (biomedcentral.com)
Overall, our study demonstrates the coordinated expression of functionally related genes during proliferation and differentiation of rainbow trout adipocyte cells. (biomedcentral.com)
In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
The ECM provides structural and biochemical support to the cells and has various other functions including cell adhesion, cell to cell communication, and differentiation [ 17 , 18 ]. (hindawi.com)
Dazl can regulate the expression, transport and localization of target mRNAs of proteins which control the differentiation, growth and maturation of germ cells. (ijbs.com)
the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)

He has been leader of a research group at the Max-Delbrück-Center for Molecular Medicine in Berlin until 2011 focusing his research on signal transduction mechanisms in human and murine embryonic stem cells. (eurostemcell.org)

The other, described in a scientific paper in Nature in March 2021, was the electronically controlled device the team had developed over seven years of trial and error for growing natural mouse embryos outside the womb. (disabled-world.com)
In the earlier research, the team successfully used this device to grow natural mouse embryos from day 5 to day 11. (disabled-world.com)
Even in the case of mice, certain experiments are currently unfeasible because they would require thousands of embryos. (disabled-world.com)
In this issue of the JCI , Chang, Sharan, and colleagues describe a novel system to evaluate human BRCA1 alleles for in vivo function using BACs containing human BRCA1 vectors in mouse cells and embryos (see the related article beginning on page 3160). (jci.org)
NBC involves molecular biology approaches and techniques involving mouse embryonic stem cells and mouse embryos that fall within the technical repertoire of laboratories addressing neurobiological and developmental questions. (researchsquare.com)
When in culture, a small fraction of these cells exhibit at any given time the gene expression pattern of 2-cell stage embryos, before cycling back to the features of more advanced embryonic cells. (news-medical.net)
This prevented zygotic genome activation altogether, and precluded the growth of embryos beyond the first couple of cell divisions. (news-medical.net)
Background: The transcription factor B-Myb is present in all proliferating cells, and in mice engineered to remove this gene, embryos die in utero just after implantation due to inner cell mass defects. (harvard.edu)

We discuss what is known about the distinct metabolic states captured in vitro by the 2-cell-like, naïve, blastocyst-like, formative, and primed states of pluripotency. (conditionmed.org)
2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
We have previously shown that fish primary preadipocytes differentiate into mature adipocytes in vitro and that these cells represent a very helpful model system to study adipose tissue development in fish [ 5 , 6 ]. (biomedcentral.com)
However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)

and from 2002 on pluripotency in embryonic stem cells in the lab of Ali H. Brivanlou. (eurostemcell.org)
We explore the recently described metabolic surge event that occurs as pluripotency is lost and stem cells commit to differentiate. (conditionmed.org)
Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)
Using RT-PCR, Western blotting and immunostaining, we show that synemin M is present at both mRNA and protein levels in undifferentiated ES cells as early as pluripotency factor Oct-3/4 and IF keratin 8. (biomedcentral.com)

E and F) Western blot analyses for p16 in FVB (E) and B6 (F) mouse embryonic fibroblasts. (koreamed.org)

In order to understand the development of adiposity, it is crucial to identify the factors and mechanisms that regulate the recruitment of mesenchymal stem cells (MSCs) of the vascular stromal fraction of the adipose tissue and its transformation into lipid-filled adipocytes. (biomedcentral.com)
Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)

Here we combine theory and experiment to analyze the dynamics of euchromatin organization as pluripotent zebrafish cells exit mitosis and begin transcription. (nature.com)
Here, we combine experiments in pluripotent zebrafish cells and theory to show that euchromatin is organized in line with an active microemulsion model. (nature.com)
The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
Cerebral organoids have a lot of potential in personalized medicine and therapy when developed from patient-specific induced pluripotent stem cells (iPSCs). (rnabiologylab.co.in)
This led us to question whether excessive Ca V 3 channels, retained mistakenly in engineered human-induced pluripotent stem cell-derived islet (hiPSC-islet) cells, act as an obstacle to hiPSC-islet maturation. (mdpi.com)

The p16(Ink4a) and p19(Arf) knockout mice were generated via transcription activator-like effector nucleases (TALENs), and p27(Kip1) knockout mice via clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRISPR/Cas9). (koreamed.org)
Figure 3 CRISPR/Cas9-mediated generation of B6-p27Kip1 knockout mouse model. (koreamed.org)
A) Representative view of the clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRISPR/Cas9) targeting strategy for generation of p27Kip1 KO mouse. (koreamed.org)
We describe all experimental steps from genetically modifying a locus of interest, via CRISPR/Cas9 editing in mouse embryonic stem cells (ESCs), to generating chimeric mice with ESC-reconstituted forebrain regions that can be directly analyzed. (researchsquare.com)
The final piece of evidence came when the EPFL scientists removed the DUX gene from fertilized mouse oocytes using CRISPR/Cas9 genome editing. (news-medical.net)
Our lab focuses on engineering highly specific and effective CRISPR-Cas9-based genome editing tools, mainly with the use of enhanced FnCas9 and base editing for developing curative therapies for Sickle Cell Disease, Duchenne Muscular Dystrophy, FENIB, etc. (rnabiologylab.co.in)
In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. (cyberleninka.org)
Harvested GS cell colonies showed a high targeting efficiency with both TALEN and CRISPR/Cas9. (cyberleninka.org)
TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure. (cyberleninka.org)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)

The method opens new vistas for studying how stem cells self-organize into organs and may help produce transplantable tissues in the future. (disabled-world.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The fertilized oocyte is totipotent, with resultant blastomeres capable of generating all three primary germ layers, ectoderm, mesoderm, and endoderm, in addition to the extra embryonic tissues. (conditionmed.org)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)

These findings represent the first evidence that synemins could potentially be useful markers for distinguishing multipotent ES cells from undifferentiated neural stem cells and more committed progenitor cells. (biomedcentral.com)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)

But going in the opposite direction, causing stem cells to differentiate into specialized body cells, not to mention form entire organs, has proved much more problematic. (disabled-world.com)
Stem cells are unique due to their ability to limitlessly self-renew and differentiate into each cell type in the adult body. (biolegend.com)
The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)

Single-cell RNA-sequencing (scRNA-seq) analysis of thousands of DIPG tumor cells containing H3K27M mutations showed that most of these tumor cells resemble oligodendrocyte precursor cells (OPC-like)-a cell type that exhibits obviously enhanced proliferation and tumor-propagating potential compared with other cell subsets in tumor samples 9 . (nature.com)
Along with the labs of Souvik Maiti and Beena Pillai , we are testing small molecules involved in the growth and proliferation of mouse organoids. (rnabiologylab.co.in)
The remaining follicular cells develop into a corpus luteum that produces E and progesterone (P). In turn, P promotes more proliferation within the uterus and vagina, and cornification in the vagina. (biomedcentral.com)
By contrast, the combination of UHRF1 depletion and histone deacetylase (HDAC) inhibition reactivated the silenced genes and strongly suppressed CRC cell proliferation. (biomedcentral.com)
However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)

Our in vivo analysis also confirmed the expression of synemins H/M in multipotent neural stem cells in the subventricular zone of the adult brain, a neurogenic germinal niche of the mice. (biomedcentral.com)
We addressed this question by using the anterior chamber of the eye (ACE) of immunodeficient mice as a site for recapitulation of in vivo hiPSC-islet maturation in combination with intravitreal drug infusion, intravital microimaging, measurements of cytoplasmic-free Ca 2+ concentration ([Ca 2+ ] i ) and patch clamp analysis. (mdpi.com)
In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)

The blastocyst comprises the inner cell mass (ICM), which gives rise to the three primary germ layers and consequently the fetus, and the trophectoderm (TE), which gives rise to the extraembryonic and placental tissue. (conditionmed.org)
DAZ family proteins are found almost exclusively in germ cells in distant animal species. (ijbs.com)
Depending on the species, DAZL is expressed in primordial germ cells (PGCs) and/or pre-meiotic and meiotic germ cells of both sexes. (ijbs.com)
DAZ family proteins are located in the nucleus and/or in the cytoplasm of male and female germ cells at different developmental stages throughout the gametogenesis. (ijbs.com)

This selectivity suggests that the commitment of CNS precursor cells to form glia or neuron involves the direct regulation of the single synemin gene. (biomedcentral.com)

Our results lend support to the concept of post-transcriptional RNA operons, but we further present evidence that nuclear proximity of genes may provide substantial functional regulation in mammalian single cells. (nih.gov)
Here, analysis of IKK-dependent substrates in CRC cells after UV treatment revealed that phosphorylation of BRD4 by IKK-α is required for its chromatin-binding at target genes upon DNA damage. (bvsalud.org)
The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
De Iaco also found that when DUX4 is forcibly produced in muscle cells, it turns on a whole set of genes that are expressed during zygotic genome activation. (news-medical.net)
The combination of UHRF1 depletion and HDAC inhibition also induced marked changes in the gene expression profiles such that cell cycle-related genes were strikingly downregulated. (biomedcentral.com)
In fact, this was shown to be possible by the transfection of genes into GS cells (Kanatsu-Shinohara et al. (cyberleninka.org)

We show here that Zbtb38 is downregulated under endoplasmic reticulum (ER) stress, which promotes ER stress-associated apoptosis in human bone marrow neuroblastoma cells. (oncotarget.com)

The different expression of isoforms H, M and L of synemin in the nervous system raises several questions about the regulation of synemin gene expression during the determination of glial and neuronal cell lineages in the central and the peripheral nervous system (CNS and PNS). (biomedcentral.com)
Many investigators once believed that TSE agents infected mainly, if not exclusively, cells of neuronal and Author affi liations: University of Edinburgh, Easter Bush, UK, (P. lymphoid lineages. (cdc.gov)

In organisms that reproduce sexually, embryonic development is the part of the life cycle that begins just after the fertilization of the female egg cell by the male sperm cell. (disabled-world.com)
An egg meets a sperm - a necessary first step in life's beginnings and a common first step in embryonic development research. (disabled-world.com)
The thymus plays a central role in the development of the body's immune system as it is the main site in which T cells are generated. (eurostemcell.org)
Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
2020). This places metabolism at the forefront of development and cell state decisions. (conditionmed.org)
Thus, we provide a reference dataset describing the expression patterns of RPRM gene products during zebrafish and mouse development as a first step to approach the physiological role of the RPRM gene family. (frontiersin.org)
RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)
Pax6 is a transcription factor present during embryonic development. (biolegend.com)
Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)
Alberto de Iaco, a postdoc in the lab of Didier Trono at EPFL, drew upon a seemingly irrelevant study of patients suffering from a form of muscular dystrophy where mutations lead to the production in muscle cells of a protein called DUX4, which is normally detected only at the earliest stage of human embryonic development. (news-medical.net)
To confirm this, the researchers analyzed publicly available data to determine what components of the human genome are expressed during the first few days of embryonic development. (news-medical.net)
Our analysis of mouse development from embryonic day 5 to 15 (E5 to E15) has demonstrated that synemin M mRNA is produced at E5 as early as nestin and vimentin mRNA, prior to the appearance of the H isoform. (biomedcentral.com)
These results suggest that maintaining MPF activity after cryopreservation may have a positive effect on further embryonic development, but is unable to fully overcome cryoinjury. (molcells.org)
Adipogenesis has been described as a two-step developmental process consisting on the commitment of undifferentiated MSCs into preadipocytes and the further development of these cells into fully functional mature adipocytes [ 4 ]. (biomedcentral.com)
We show that the enzymatic acetylation and deacetylation of a cell surface carbohydrate controls B cell development, signaling, and immunological tolerance. (harvard.edu)
All cell lines we studied resisted between accumulation of PrPTSE and the development infection with 3 TSE agents, including the BSE agent. (cdc.gov)

Mutations in cyclin-dependent kinase inhibitors controlling the G1 phase of the cell cycle are prevalent in various cancers. (koreamed.org)
Mice lacking the tumor suppressors p16(Ink4a) (Cdkn2a, cyclin-dependent kinase inhibitor 2a), p19(Arf) (an alternative reading frame product of Cdkn2a,), and p27(Kip1) (Cdkn1b, cyclin-dependent kinase inhibitor 1b) result in malignant progression of epithelial cancers, sarcomas, and melanomas, respectively. (koreamed.org)
Here, we generated knockout mouse models for each of these three cyclin-dependent kinase inhibitors using engineered nucleases. (koreamed.org)

Our study shows a developmental stage specific regulation of synemin isoforms in ES cells and its neural derivatives. (biomedcentral.com)
Insulin produces the dephosphorylation of only a small subset of proteins at discrete locations, whereas PP1 is ubiquitously expressed and is found in virtually all cellular compartments, suggesting that mechanisms exist for the targeted regulation of PP1 in insulin-responsive cells that selectively permit activation of the enzyme only at these sites. (jci.org)

He explains that scientists already know how to restore mature cells to "stemness" - pioneers of this cellular reprogramming had won a Nobel Prize in 2012. (disabled-world.com)
Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)

In the present study, we confirmed upregulated NORAD expression levels in a set of human epithelial breast cancer cell lines (MDA-MB-231, MDA-MB-436, and MDA-MB-468), which belong to the most aggressive subtypes (triple-negative breast cancer). (bvsalud.org)
We introduced BAC clones with human wild-type BRCA1 or variants into Brca1-null ES cells and confirmed that only wild-type and a known neutral variant rescued cell lethality. (jci.org)
Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
The formation of a human embryo starts with the fertilization of the oocyte by the sperm cell. (news-medical.net)
Ubiquitin-like protein containing PHD and RING finger domains 1 (UHRF1), also known as ICBP90 in human and Np95 in mouse, plays an important role in reading and maintaining the epigenetic marks [ 4 ]. (biomedcentral.com)
No PrPTSE or vCJD infections have been transmitted by transfusions of infectivity has been detected in any exposed cell line under nonleukoreduced erythrocyte concentrates and by a human- study so far. (cdc.gov)
This approach could therefore micromass test, and the embryonic stem cell test (EST) provide a means to reduce the need for animal testing in human risk assessment practices. (cdc.gov)

We're a partnership of more than 400 stem cell and regenerative medicine labs across Europe, connected via research centres, consortia, networks and hubs. (eurostemcell.org)
Paolo De Coppi is a Consultant Paediatric Surgeon at Great Ormond Street Hospital (GOSH), and Reader and Head of Stem Cells and Regenerative Medicine at the UCL Institute of Child Health in London. (eurostemcell.org)

Unlike preexisting embryonic stem cell-based knockout mice, our mouse models are free from selectable markers or other external gene insertions, permitting more precise study of cell cycle-related diseases without confounding influences of foreign DNA. (koreamed.org)

In the mouse, a transporting epithelium is established around the 8-16-cell stage through a process known as compaction where cell definition is lost and the outer cells of the embryo form tight junctions, giving rise to the blastocyst. (conditionmed.org)
Analysis of the effectiveness of caffeine and MG132 for improving somatic cell nuclear transfer (SCNT) technology using cryopreserved eggs showed that supplementation did not improve the blastocyst formation rate of cloned mouse eggs. (molcells.org)

The study points to DUX4, and by extension the DUX family of proteins, as the master regulator responsible for kick-starting genome expression at the earliest stage of embryonic life in humans, mouse and probably all placental mammals. (news-medical.net)
Depletion of UHRF1 rapidly induced genome-wide DNA demethylation in CRC cells. (biomedcentral.com)
Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. (cyberleninka.org)
2004). In order to make GS cells more widely applicable for the study of spermatogenesis at the genetic and molecular levels, it is desirable to be able to modify their genome without disturbing their spermato-genic ability. (cyberleninka.org)

However, this technology also may have several limitations, such as the risk of contamination by external pathogens because of direct contact with liquid nitrogen and increased cell toxicity due to high CPA concentrations. (molcells.org)

In other multicellular organisms, the word "embryo" can be used more broadly to describe any early developmental or life cycle stage before birth or hatching. (disabled-world.com)

These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)

Solely from stem cells, without egg, sperm or womb, synthetic mouse embryo models were created. (disabled-world.com)
The team set out to grow a synthetic embryo model solely from naïve mouse stem cells cultured for years in a petri dish, dispensing with the need for starting with a fertilized egg. (disabled-world.com)
The method opens new horizons for studying how stem cells form various organs in the developing embryo. (disabled-world.com)
But in a Weizmann Institute of Science study published today in Cell , researchers have grown synthetic embryo models of mice outside the womb by starting solely with stem cells cultured in a petri dish - that is, without using fertilized eggs. (disabled-world.com)
Up to the 2-cell embryo, blastomeres remain totipotent (Garner and McLaren, 1974). (conditionmed.org)
EmbryoMax KSOM mouse embryo medium (Millipore, cat. (researchsquare.com)

Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)

The resulting fusion of these two cells produces a single-celled zygote that undergoes many cell divisions that make cells known as blastomeres. (disabled-world.com)

Insulin resistance in older PTG heterozygous mice correlates with a significant increase in muscle triglyceride content, with a corresponding attenuation of insulin receptor signaling. (jci.org)

In the zebrafish, rprma mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), rprmb is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas rprml is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. (frontiersin.org)
Rb , or retinoblastoma protein, is a key regulator of the cell cycle, particularly during the transition from the G1 to S phases. (biolegend.com)
PrPC is a susceptibility to infection of 5 cell lines used or proposed for nonessential protein but, at least in mice and cows, must be manufacture of biological products, as well as other lines. (cdc.gov)

A and B) Representative views of the transcription activator-like effector nuclease (TALEN) targeting strategy for generation of p16Ink4a KO mice in both FVB (A) and B6 (B) strains. (koreamed.org)

In the traumatic SCI mice, ER stress presented in injured spinal cord induced repression of Zbtb38 expression and triggered Zbtb38-mediated apoptosis. (oncotarget.com)
Restoring Zbtb38 function in injured spinal cord by injection of lentivirus containing Zbtb38 into SCI mice, significantly alleviated secondary damage of spinal cord with decreased ER stress-associated apoptosis and partially recovered spinal cord functions. (oncotarget.com)

Microarray profiling of hypothalamic gene expression changes in Huntington's disease mouse models. (lu.se)

In eukaryotes, DNA is packed inside the cell nucleus in the form of chromatin, which consists of DNA, proteins such as histones, and RNA. (nature.com)
It has been suggested that synemin could function as a linker between different cytoskeletal components based on the fact that it interacts with several proteins involved in the organization of the costameres, neuromuscular and myotendinous junctions within striated muscle cells. (biomedcentral.com)
One clue to this dilemma has emerged from the identification of "targeting" subunits that allow for the compartmentalized activation of the phosphatase, resulting in the specific dephosphorylation of discrete pools of proteins within the cell. (jci.org)

Adipogenesis has been extensively studied in mammals [ 7 ] and several cell and animal models have been successfully used to describe the chronology of the molecular events governing this process. (biomedcentral.com)

Moreover, myeloperoxidase (MPO), an abundant enzyme of inflammatory cells (neutrophils), - involved in the principal defense mechanisms of innate immunity -was also effective in oxidative biodegradation of CNT in biochemical models and in cells yielding the products that did not cause pulmonary inflammation in mice [14] . (cdc.gov)
In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)

Intermediate filaments (IFs) are major components of the mammalian cytoskeleton and expressed in cell-type-specific patterns. (biomedcentral.com)
Spermatogonial stem cells (SSCs) are pivotal for maintaining the lifelong sperm production of mammalian males. (cyberleninka.org)

Until now, in most studies, the specialized cells were often either hard to produce or aberrant, and they tended to form a mishmash instead of well-structured tissue suitable for transplantation. (disabled-world.com)
Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)
The Rosa26-targeted GS cells differentiated into fertility-competent sperm following transplantation. (cyberleninka.org)
On the other hand, Stra8-targeted GS cells showed defective spermatogenesis following transplantation, confirming its prime role in the initiation of meiosis. (cyberleninka.org)

C and D) Representative results of PCR genotyping for FVB (C) and C57BL/6 (D) strains of WT and p16Ink4a KO mice. (koreamed.org)
Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. (cdc.gov)

A schematic of the Cdkn2a locus with TALEN-binding nucleotides (blue) and the amino acid sequences (purple) of the WT (upper) and KO (lower) mice are shown. (koreamed.org)
A schematic of the Cdkn1b locus with single guide RNA target nucleotides (blue) and the amino acid sequences (purple) of the wild-type (upper) and knockout (lower) mice are shown. (koreamed.org)

This yields the zygote, the primordial cell that carries one copy each of the maternal and paternal genomes. (news-medical.net)

Hypothalamic expression of huntingtin causes distinct metabolic changes in Huntington's disease mice. (lu.se)

This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)

Simultaneous inhibition of BRAF-dependent IKK-α activity, BRD4, and the JAK/STAT pathway enhanced the therapeutic potential of 5-fluorouracil combined with irinotecan in CRC cells and is curative in a chemotherapy-resistant xenograft model. (bvsalud.org)

C) Semi-quantitative PCR analysis for p27 transcript in the liver of WT and p27Kip1 KO mouse. (koreamed.org)

Anatomy41

Organisms8

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Chemicals and Drugs80

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Phenomena and Processes76

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