• The present review discusses methods to improve oocyte quality in older women and new stimulation protocols that may improve the number of mature oocytes retrieved during an in vitro fertilization cycle. (arvigen.com)
  • They must inject themselves daily for four weeks with large doses of hormones to encourage their ovaries to produce numerous mature oocytes while simultaneously suppressing the normal menstrual cycle, which would eject the eggs into the fallopian tubes. (discovermagazine.com)
  • at the same time, others are learning how to mature oocytes outside the body. (discovermagazine.com)
  • Immature cumulus-oocyte complexes are retrieved to obtain mature oocytes by in vitro maturation (IVM), a laboratory tool in reproductive medicine to obtain mature oocytes. (biomedcentral.com)
  • To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
  • Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)
  • His PGS (Preimplantation Genetic Screening) fixation techniques has been included by ESHRE PGD Consortium (European Society of Human Reproduction and Embryology) in the best practice guidelines for clinical preimplantation genetic diagnosis (PGD) and preimplantation genetic screening (PGS) and his research on sperm genetic testing before fertilization was discussed by the Nature in 2003. (wikipedia.org)
  • Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
  • These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
  • Intracytoplasmic sperm injection ( ICSI / ˈ ɪ k s i / IK -see ) is an in vitro fertilization (IVF) procedure in which a single sperm cell is injected directly into the cytoplasm of an egg . (wikipedia.org)
  • Round spermatid injection (ROSI) is a technique of assisted reproduction whereby a round spermatid is injected into oocyte cytoplasm in order to achieve fertilization. (wikipedia.org)
  • SECM technique may be a valuable tool for accurately assessing the quality of embryos and thereby contribute to improving outcomes associated with assisted reproduction, including human in vitro fertilization. (bioone.org)
  • Following germinal vesicle break down (GVBD) the oocyte is transcriptionally quiescent until fertilization and activation of the embryonic genome, occurring at the four-cell stage of development in the pig [ 9 ]. (biomedcentral.com)
  • 11 Among women undergoing in vitro fertilization-embryo transfer in the United States, deliveries per oocyte retrieved decreased from 36.9 percent among women under the age of 35 years to 20.5 percent among women between the ages of 38 and 40 years and to 10.7 percent among women between the ages of 41 and 42 years. (ldysinger.com)
  • To ascertain whether the Kunming (KM) mouse is an available model for age-related decline in female fertility in human or not, oocytes from young (6-8 weeks), middle-aged (9 months) and aged (12 months) female mice were compared with respect to number of oocytes, frequency of in-vitro maturation (IVM) and in-vitro fertilization (IVF), and meiotic chromosome segregation and alignment. (cambridge.org)
  • Quality control in human in vitro fertilization. (shengsci.com)
  • The implementation of suitable quality control (QC) is not only required for the accreditation of a human in vitro fertilization (IVF) laboratory, but is also fundamental to its success. (shengsci.com)
  • On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential cellular functions including the function of germ cells (oocytes and spermatozoa) and thereby the fertilization process. (hindawi.com)
  • However, these techniques have to be carried out very carefully as they, if applied wrongly, bear risks of generating ROS damaging the germ cells and preventing fertilization. (hindawi.com)
  • This has been shown for intrauterine insemination (IUI), in vitro fertilization (IVF), and ICSI, and increased incidences of chromosomal abnormalities, minor and major birth defects, or early childhood cancers, particularly in the male offspring, have been linked to these procedures [ 8 - 16 ]. (hindawi.com)
  • When it comes to eggs, though, the success of in vitro fertilization has created a demand far exceeding supply. (discovermagazine.com)
  • Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs [3] "Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. (edu.au)
  • Recurrent implantation failure (RIF) refers to cases in which women have had three failed in vitro fertilization (IVF) attempts with good quality embryos. (biomedcentral.com)
  • Although there is no accepted formal definition for recurrent implantation failure, Orvieto suggests that it is after three failed in vitro fertilization-embryo transfer (IVF-ET) cycles with good quality embryos transferred [ 5 ]. (biomedcentral.com)
  • The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)
  • Injection of a MIR21 mimic increased oocyte maturation rate. (biomedcentral.com)
  • Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)
  • In particular, he is best known for his studies of in vitro fertilisation and embryo transfer. (wikipedia.org)
  • 2001 Jul;76:186-8 Nucleoli in a pronuclei-stage mouse embryo are represented by major satellite DNA of interconnecting chromosomes. (wikipedia.org)
  • The suitability of one assay in particular, the mouse embryo assay (MEA), has been questioned over the years. (shengsci.com)
  • A human female embryo develops around 7 million proto-eggs, known as primordial oocytes. (discovermagazine.com)
  • In addition, we determined that oocytes surrounded by their follicular cells expressing EGFR-Grb7 exhibit normal meiotic resumption. (bvsalud.org)
  • The oocyte must maintain arrest at the diplotene stage until meiotic resumption occurs. (biomedcentral.com)
  • This study may be an important supplement for the mechanism of oocyte meiotic resumption and provide new diagnostic targets and treatment clues for infertility patients with oocyte maturation disorder. (cambridge.org)
  • The decreased quality of oocytes involves an increased rate of chromosomal aneuploidy with aging predominantly related to meiotic errors during oocyte maturation. (arvigen.com)
  • As atresia continues, both the number and quality of oocytes fall below a critical level, and the rate of aneuploidy increases - a finding that is related at least in part to problems of the meiotic spindle 15 , 16 resulting in nondisjunction. (ldysinger.com)
  • Germinal vesicle breakdown (GVBD) occurs during oocyte meiotic maturation, a period when transcriptional processes are virtually inactive. (biomedcentral.com)
  • The mean number of ovarian follicles, ovarian germinal vesicle oocytes and ovulated MII oocytes decreased significantly with maternal age. (cambridge.org)
  • Significant reduction of germinal vesicle (GV) and MII oocytes and significant increase of metaphase chromosome misalignment and premature chromatid segregation after meiotic maturation of oocytes, similar to human, presumably contribute to the decline in aged KM mice. (cambridge.org)
  • The germinal vesicle breakdown (GVBD) is a landmark event of oocyte maturation. (cambridge.org)
  • In our previous studies, we found that zona pellucida 3 (ZP3) was strongly concentrated in the nuclear region of germinal vesicle (GV) oocytes and interacted with aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) and lamin A to promote GVBD. (cambridge.org)
  • 2020 Nov;114(5):943-944 Term oocyte maturation and term ovarian stimulation: impact on oocyte competence. (wikipedia.org)
  • in 2015 showed that supplementation of CoQ10 in an aged animal model delayed decline in ovarian reserve, restored oocyte mitochondrial gene expression, and improved mitochondrial activity with a significant reduction in oocyte aneuploidy. (arvigen.com)
  • 3 Although ovarian aging in mice has a hypothalamic component, this has not been found to be the case in humans. (ldysinger.com)
  • Dozortsev is also credited with and introducing Term Stimulation (TM), a method to improve quality of the oocytes by increasing duration of the follicular phase. (wikipedia.org)
  • Further, melatonin and its receptors play a significant role in antioxidant defense mechanism of ovary for follicular growth and maturation. (jrhm.org)
  • Xenopus oocytes are encompassed by a layer of follicular cells that contribute to oocyte growth and meiosis in relation to oocyte maturation. (bvsalud.org)
  • However, the effects of the interaction between follicular cells and the oocyte surface on meiotic processes are unclear. (bvsalud.org)
  • Here, we investigated Xenopus follicular cell function using oocyte signaling and heterologous-expressing capabilities. (bvsalud.org)
  • We found that oocytes deprotected from their surrounding layer of follicular cells and expressing the epidermal growth factor (EGF) receptor (EGFR) and the Grb7 adaptor undergo accelerated prophase I to metaphase II meiosis progression upon stimulation by EGF. (bvsalud.org)
  • Furthermore, an O-GlcNAcylation increase (by inhibition of O-GlcNAcase), the glycosidase that removes O-GlcNAc moieties, or decrease (by inhibition of OGT) amplifies oocyte spindle defects when follicular cells are absent highlighting a control of the meiotic spindle by the OGT-O-GlcNAcase duo. (bvsalud.org)
  • In summary, our study provides further insight into the role of the follicular cell layer in oocyte meiosis progression. (bvsalud.org)
  • Maturation of the mammalian oocyte is a complex process involving internal checkpoints and bidirectional communication with the surrounding cumulus cells. (biomedcentral.com)
  • It is well known that c-Jun N-terminal kinase (JNK) plays pivotal roles in various mitotic events, but its function in mammalian oocyte meiosis remains unknown. (cambridge.org)
  • In this study, by whole-exome sequencing (WES), we identified homozygous and compound heterozygous MAD2L1BP variants in three families with female patients diagnosed with primary infertility owing to oocyte metaphase I (MI) arrest. (bvsalud.org)
  • Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)
  • Oocytes were considered mature when they reached metaphase II. (sciencegate.app)
  • Female infants have 6 to 7 million oocytes at 20 weeks of gestation, when progressive atresia occurs, resulting in 1 to 2 million oocytes at birth, approximately 25,000 at the age of 37 years, and 1000 at the age of 51 years, the average age of natural menopause in the United States. (ldysinger.com)
  • By puberty there are at most only a quarter of a million oocytes left. (discovermagazine.com)
  • This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal uterus. (wikipedia.org)
  • If all requirements for round spermatid selection and injection are successfully met, the injected oocytes develop to early embryos and can be transferred to the mother's uterus to produce pregnancy. (wikipedia.org)
  • In this paper, we describe a scanning electrochemical microscopy (SECM) technique that is a non-invasive and sensitive method for measuring oxygen consumption by individual embryos. (bioone.org)
  • MicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. (bvsalud.org)
  • Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes and preimplantation embryos, and the effect of these changes on the future of offspring is unknown, and has caused concerns. (bvsalud.org)
  • The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)
  • ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. (sciencegate.app)
  • Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
  • The spindle assembly checkpoint (SAC) is an intricate surveillance mechanism that ensures accurate segregation of chromosomes throughout cell cycles. (bvsalud.org)
  • It mediates proper CHROMOSOME SEGREGATION and contractile ring function during CYTOKINESIS. (lookformedical.com)
  • Aurora kinases are found in all organisms in which their function has been conserved throughout evolution, namely the control of chromosome segregation. (lookformedical.com)
  • According to In-Vitro Fertilisation website source, "In 1995, Dmitri Dozortsev and his coworkers A. Rybouchkin, Petra De Sutter, C. Qian, M. Dhont have discovered that oocyte activation during ICSI is triggered by a water-soluble, heat-sensitive, non species specific cytosolic sperm factor. (wikipedia.org)
  • In terms of insemination, ICSI needs only one sperm cell per oocyte , while IVF needs 50,000-100,000. (wikipedia.org)
  • Even though many technical aspects of ROSI are similar to those of ICSI, there are also significant differences between both techniques. (wikipedia.org)
  • [2] The microinjection procedure for ROSI also differs slightly from that of ICSI, since additional stimuli are needed to ensure proper oocyte activation after spermatid injection. (wikipedia.org)
  • Oogonia enter meiosis during embryonic development, becoming oocytes. (wikipedia.org)
  • Understanding the biological functions of miRNAs in frozen maturated oocytes may provide a better understanding of embryonic development and a comparison of fertility conservation in female mammals. (bvsalud.org)
  • Oocyte meiosis, important to all animal life cycles yet unlike all other instances of animal cell division, occurs completely without the aid of spindle -coordinating centrosomes . (wikipedia.org)
  • Removal of the oocyte from the follicle causes meiosis to progress in the oocyte. (wikipedia.org)
  • As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)
  • [5] Because the granulosa cells and oocyte are connected by gap junctions, cyclic GMP also decreases in the oocyte, causing meiosis to resume. (wikipedia.org)
  • In those men in whom spermatogenesis is blocked at the stage of round spermatids, in which meiosis has already been completed, these round cells can successfully fertilize oocytes after being injected into their cytoplasm. (wikipedia.org)
  • It was found that FIGNL1, a member of MTSPs, was predominantly expressed in mouse oocytes and distributed at the spindle poles during meiosis in the present study. (cambridge.org)
  • Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
  • It has been hypothesized that in gonadal dysgenesis various deletions on the X chromosome cause such rapid atresia of oocytes 5 that few girls with this condition reach puberty with functioning ovaries. (ldysinger.com)
  • Gasic, S., Mihola, O. & Trachtulec, Z. Prdm9 deficiency of rat oocytes causes synapsis among non-homologous chromosomes and aneuploidy. (cuni.cz)
  • 1995 Nov;10(11):2960-4 Human oocyte activation following intracytoplasmic injection: the role of the sperm cell. (wikipedia.org)
  • This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
  • Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)
  • One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)
  • The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)
  • The multi-subunit shelterin complexes bind along mammalian telomeres, shielding the natural chromosome ends from engaging the DNA damage signaling and repair machinery [ 1 ]. (prolekarniky.cz)
  • Therefore, in future, melatonin can be implicated as preferable therapeutic especially in IVF and assisted reproductive techniques. (jrhm.org)
  • In addition, other corrective measures include sperm separation for assisted reproductive techniques. (hindawi.com)
  • Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
  • Inhibition of nuclear meiotic maturation via IBMX significantly increased MIR21 and decreased its target, PDCD4. (biomedcentral.com)
  • AIPL1 was also proved to accumulate in the GV region of oocytes, and ZP3 deletion can significantly inhibit the aggregation of AIPL1 in the nuclear region. (cambridge.org)
  • Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)
  • In the oocyte, cyclic GMP prevents the breakdown of cyclic AMP by the phosphodiesterase PDE3, and thus maintains meiotic arrest. (wikipedia.org)
  • an investigation of their specific localization in human brain and their influence on CaV2.1 voltage-dependent calcium channels expressed in Xenopus oocytes. (biomedcentral.com)
  • In addition, we investigated whether human γ 2 or γ 4 could modulate the electrophysiological properties of a neuronal VDCC complex transiently expressed in Xenopus oocytes. (biomedcentral.com)
  • When co-expressed in Xenopus oocytes with a Ca V 2.1/β 4 VDCC complex, either in the absence or presence of an α2δ 2 subunit, neither γ 2 nor γ 4 significantly modulated the VDCC peak current amplitude, voltage-dependence of activation or voltage-dependence of steady-state inactivation. (biomedcentral.com)
  • In 1913, as an undergraduate, Sturtevant created one of the earliest genetic maps of a fruit fly chromosome, which showed the relative positions of genes along the chromosome. (asu.edu)
  • 1 , 2 The mechanism underlying this process is poorly understood and involves multiple factors encoded by genes on the X chromosome, as well as on autosomes. (ldysinger.com)
  • Despite considerable ongoing effort toward the identification of chromosome regions affected in autism and the characterization of many potential gene candidates, only a few genes have been reproducibly shown to display specific mutations that segregate with autism, likely because of the complex polygenic nature of this syndrome. (neurotransmitter.net)
  • Among those, several candidate genes have been shown to control the early patterning and/or the late synaptic maturation of specific neuronal subpopulations controlling the balance between excitation and inhibition in the developing cortex and cerebellum. (neurotransmitter.net)
  • Thus, the maturing oocyte is reliant on processes such as post-transcriptional gene regulation (PTGR) to regulate the mRNA and protein repertoire. (biomedcentral.com)
  • The inability to transcribe mRNA during this stage of development and the probable necessity for post-transcriptional gene regulation (PTGR) suggests an important role for non-coding RNA in the maturing oocyte. (biomedcentral.com)
  • As a follow-up study, conditional disruption of the gene PDSS-2, resulting in isolated CoQ deficiency in the oocytes of young animals, resulted in phenotypic changes characteristic of oocyte mitochondrial dysfunction associated with aging. (arvigen.com)
  • We are investigating how female lymphocytes maintain X-chromosome Inactivation, which is an epigenetic process responsible for equalizing gene expression between sexes. (upenn.edu)
  • The mouse Tzfp gene, mapped to chromosome 7 B2-B3, encodes a 465-amino acid transcription factor containing a conserved N-terminal BTB/POZ domain and three C-terminal PLZF-like C(2)H(2) zinc fingers. (lookformedical.com)
  • Vitrifaction by ultrarapid cooling and warming is considered a standard approach in cattle oocyte preservation. (jove.com)
  • Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
  • As a result, these aged mice had more oocytes with stimulation, the developmental potential of the oocytes was improved, and more offspring were born compared to old animals receiving placebo 1. (arvigen.com)
  • The Human Genome Project (HGP) was an international scientific effort to sequence the entire human genome, that is, to produce a map of the base pairs of DNA in the human chromosomes, most of which do not vary among individuals. (asu.edu)
  • Human oocyte maturation arrest represents one of the severe conditions for female patients with primary infertility. (bvsalud.org)
  • Together, our studies identified and characterized novel biallelic variants in MAD2L1BP responsible for human oocyte maturation arrest at MI, and thus prompted new therapeutic avenues for curing female primary infertility. (bvsalud.org)
  • To characterize the effect of artificially increasing MIR21 on oocyte competence without inhibiting GVBD, a MIR21 mimic, scrambled microRNA negative control, or nuclease free water was micro-injected into denuded oocytes at 21 h of IVM. (biomedcentral.com)
  • Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
  • However, as the oocyte grows, these proteins are synthesized, and meiotic arrest becomes dependent on cyclic AMP . (wikipedia.org)
  • Once the kinetochores of chromosomes are correctly attached to bipolar spindles and the SAC is satisfied, the MAD2L1BP, best known as p31comet, binds mitosis arrest deficient 2 (MAD2) and recruits the AAA+-ATPase TRIP13 to disassemble the mitotic checkpoint complex (MCC), leading to the cell-cycle progression. (bvsalud.org)
  • Oocyte arrest at the diplotene stage is maintained in part through the activity of phosphodiesterase enzymes that catalyze the hydrolysis of cyclic adenosine monophosphate (cAMP) into AMP [ 1 , 2 ]. (biomedcentral.com)
  • Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes , a process called oocytogenesis . (wikipedia.org)
  • Injection of myc-tagged JNK2α1 mRNA into oocytes also revealed its localization on spindle poles. (cambridge.org)
  • This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
  • In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
  • In person suffering from diabetes mice in vivo, subnormal intraretinal subscriber base regarding manganese was significantly improved by simply catalase supplementing. (hsp90-inhibitors.com)
  • However, DFMO had been mainly without effect of these inside vitro or even in vivo assays. (hsp90-inhibitors.com)
  • cRNA microinjection of full-length or truncated MAD2L1BP uncovered their discordant roles in driving the extrusion of polar body 1 (PB1) in mouse oocytes. (bvsalud.org)
  • Furthermore, the patient's oocytes carrying the mutated MAD2L1BP resumed polar body extrusion (PBE) when rescued by microinjection of full-length MAD2L1BP cRNAs. (bvsalud.org)
  • In this video, we describe a protocol for the vitrification of sheep oocyte, collected from both juvenile and adult donors, and in vitro matured to prior to cryopreservation. (jove.com)
  • Our laboratory investigates X-chromosome Inactivation, and how this epigenetic process contributes to female-biased autoimmunity. (upenn.edu)
  • 2000 Feb;73(2):366-71 Sperm-associated oocyte-activating factor is released from the spermatozoon within 30 minutes after injection as a result of the sperm-oocyte interaction. (wikipedia.org)
  • 2003 Oct-Nov;7(4):392-9 An improved fixation technique for fluorescence in situ hybridization for preimplantation genetic diagnosis. (wikipedia.org)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
  • Decline in the Number of Oocytes from Birth to Menopause. (ldysinger.com)
  • The study suggests that KM mouse exhibits an age-related decline in female fertility. (cambridge.org)
  • The low maturation rate of oocytes is an important reason for female infertility and failure of assisted pregnancy. (cambridge.org)
  • Afterward they transplanted the thawed strips into mice that, thanks to genetic engineering, lacked an immune system and so couldn't reject the foreign tissue. (discovermagazine.com)
  • Here we describe the cloning and characterization of the developmental expression pattern of two mouse cdc25 homologs. (biologists.com)
  • Over the past half century, this technique has been used with increasing frequency to treat numerous malignant and nonmalignant diseases. (medscape.com)
  • Works in various organisms have revealed that the kinase is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. (lookformedical.com)
  • CoQ10 administration for 12-16 weeks in mice is likely equivalent to years of use in humans and thus additional large-scale clinical studies are needed. (arvigen.com)
  • In spite of the abundance of research on the roles of TE on the genome biology in humans, model organisms (e.g., mice and Drosophila ), and plants (especially crop species), few studies on TE have been conducted in livestock and poultry. (biomedcentral.com)
  • The TPP1/POT1 heterodimer also promotes telomerase processivity, as demonstrated by an in vitro direct telomerase activity assay [ 4 , 20 ]. (prolekarniky.cz)
  • When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
  • The rate of IVM in oocytes from young mice (73.9%) was less significantly than that in oocytes from middle-aged and aged mice (86.1% and 84.4%, respectively). (cambridge.org)
  • Immunocytochemical analysis showed that ageing caused a significantly higher rate (49.3%) of chromosome misalignment than that (15.7%) of the young mice. (cambridge.org)
  • This study was aimed to determine the effect of urea in maturation medium on in vitro oocyte maturation rate. (sciencegate.app)
  • The fact that live-birth rates from oocyte donation in older women are consistent with the age of the donor suggests that oocyte quality is the major factor responsible for reduced fecundability with aging. (arvigen.com)
  • In vitro maturation allows female goat (does) contributes toward reproduction despite the fact that the animal has been slaughtered. (sciencegate.app)