Mouse cell genome mice knockout. Medical search. Frequent questions

Using mice, the International Mouse Phenotyping Consortium (IMPC) aims to target each protein-coding gene and phenotype multiple organ systems in single-gene knockout models by a global network of mouse clinics. (springer.com)
The International Mouse Phenotyping Consortium, IMPC, has set the goal of systematically "knocking out" each protein-coding genes in the mouse and phenotyping these so-called knockout (KO) mice using highly standardized high-throughput tests (Brown and Moore 2012 ). (springer.com)
The International Mouse Phenotyping Consortium project is systematically phenotyping knockout mice from the mutant ES cells produced by the International Mouse Knockout Consortium. (jax.org)

Experimental results of another study showed that obese mice had an over expression of both CADM1 and CADM2 genes, and that loss of CADM1 protected mice from obesity, promoting a negative energy balance and weight loss. (wikipedia.org)
Beyond that, we are linking metabolism to the heart and describing phenotypes that emerge in a set of known genes, when knocked out in mice, such as the leptin receptor ( Lepr ), leptin ( Lep ), and Bardet-Biedl syndrome 5 ( Bbs5 ). (springer.com)
Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. (ubigene.us)
Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)
Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
In adipocyte Senp2-de﫿ciency mice, accumulation of the SUMOylated Setdb1 suppressed the expression of Pparg and Cebpa genes as well as lipid metabolism-related target genes, which would decrease the ability of lipid storage in adipocytes. (deepdyve.com)
adqcKO Mechanistically, adipocyte Senp2 de﫿ciency caused the downregula- Senp2 mice exhibit an ectopic lipid accumulation and tion of Pparg and Cebpa as well as their downstream target genes insulin resistance related to lipid storage. (deepdyve.com)
With CRISPR-Cas9, researchers like Latham can precisely edit specific sections of a cell's genome by removing, adding or altering genes. (wsu.edu)
Mario Capecchi and Oliver Smithies were both seeking ways of specifically altering the mammalian genome, Capecchi with a view to inserting new genes into cells and Smithies in the hope of correcting genetic defects that lead to disease. (nobelprize.org)
Combining the two, by modifying genes in embryonic stem cells and then injecting those cells into fertilized mouse eggs, made it possible to rear mice with discrete genetic modifications that would be inherited between generations. (nobelprize.org)
In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. (cyberleninka.org)
In fact, this was shown to be possible by the transfection of genes into GS cells (Kanatsu-Shinohara et al. (cyberleninka.org)
The members of the International Knockout Mouse Consortium are working together to mutate all protein-coding genes in the mouse using a combination of gene trapping and gene targeting in C57BL/6 mouse embryonic stem (ES) cells. (tigm.org)
We present a sensitive approach to predict genes expressed selectively in specific cell types, by searching publicly available expression data for genes with a similar expression profile to known cell-specific markers. (biomedcentral.com)
It is these differentially expressed genes that frequently control cell differentiation, define cell-specific phenotypes, and provide the core signature of cell identity. (biomedcentral.com)
By focusing on identifying differentially expressed genes, it turns a more complex model-fitting problem into a classification problem [ 9 ], opening the door to algorithms that may be more sensitive, especially for rare and difficult-to-isolate cell types. (biomedcentral.com)
However, these algorithms all require very large training sets of both positive and negative control genes (≥10 of each) to define any cell type. (biomedcentral.com)
This requirement poses a severe limitation for most biological applications, as it is difficult to curate such a large list of established marker genes for even well-studied cell types and impossible for many others. (biomedcentral.com)
Here, we present CellMapper , an algorithm optimized for sensitive identification of cell type-enriched genes using as little as a single query gene. (biomedcentral.com)
We then apply our algorithm to a large compendium of 19,801 microarrays and identify genes specifically expressed in 30 diverse cell types of widespread importance in human biology, demonstrating that CellMapper can be readily used for cell types from many different tissues. (biomedcentral.com)
Finally, we explore a clinically relevant application to prioritize candidate genes in loci identified by genome-wide association studies (GWAS). (biomedcentral.com)
CellMapper takes as input (1) a large set of gene expression data and (2) a query gene (or genes) specifically expressed in the cell type of interest and then estimates the probability that every other gene in the dataset is co-expressed with the query gene (Fig. 1a ). (biomedcentral.com)
These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
Moreover, genome instability was attributed to impaired autophagy and several autophagy genes with tumor suppressor functions (e.g. (hindawi.com)
In a genome-wide screen for putative tumor suppressor genes, the EBF3 locus on the human chromosome 10q26.3 was found to be deleted or methylated in 73% of the examined cases of brain tumors. (aacrjournals.org)
The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
They found that 1,084 genes in 129 strain mice have insertions, deletions, or single-nucleotide mutations that cause them to diverge in sequence from Black 6 mice. (the-scientist.com)
Germline transmission breeding and subsequent backcrosses to C57BT/6 (M6) to generate congenic stresses delay practical studies and increase costs, while at the same time genes closely linked to the unique targeted locus remain from the unique Sera cell genome and may confound study results [4]. (liveconscience.com)
Collectively, these considerations led the large level mouse mutagenesis projects structured under the umbrella Aliskiren hemifumarate of the World Knockout Mouse Consortium (IKMC) to select M6-produced Sera cells as the parental lines in which to mutate all Aliskiren hemifumarate protein coding genes of Aliskiren hemifumarate mouse [5], [6]. (liveconscience.com)
The insulin-producing cells that we can make from these patients can then be used to understand the mechanisms by which these risk genes cause dysfunction of beta-cells. (lu.se)
Whole genome analysis of lung, sampled 24 hours after challenge, showed expression changes of not only genes belonging to several Gene Ontology groups with up-regulation of inflammatory-associated genes and those associated with lung remodeling but also genes involved in downsizing these processes. (cdc.gov)

Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
This parallel detection of PB1-F2 and NP suggests that applied sensor chip technology may be amenable to an arrow immunosensor for simultaneous detection of all known influenza virus proteins in infected tissues and cells. (omicsonline.org)
Autophagy is a cellular catabolic process that eliminates damaged cell organelles, unfolded proteins, and various intracellular pathogens through lysosomal degradation. (hindawi.com)
During elongation and maturation, the phagophore encapsulates damaged proteins and cell organelles. (hindawi.com)

International Cancer Genome Consortium. (cancerindex.org)
The Mouse Genome Informatics group is a founding member of the Gene Ontology Consortium . (tigm.org)
The International Gene Trap Consortium (IGTC) represents all publicly available gene trap cell lines, which are available on a non-collaborative basis for nominal handling fees. (tigm.org)

Exclusive Red Cotton system（en.rc-crispr.com）was used to design the sgRNA fragment knockout strategy. (ubigene.us)
Two sgRNAs were constructed into CRISPR-U™ plasmid which will be subsequentially transfected into cells by electroporation/virus method. (ubigene.us)
This course will provide training in genome editing and cell engineering in mammalian cells and mouse embryos using the highly efficient CRISPR/Cas9 system. (sb-3.org)
This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and who want to learn how to create an engineered mammalian cell line or mouse model using the most recent and advanced CRISPR/Cas9 system. (sb-3.org)
The EMBL course about CRISPR applications for genome engineering was an incredible experience. (sb-3.org)
Harvested GS cell colonies showed a high targeting efficiency with both TALEN and CRISPR/Cas9. (cyberleninka.org)
TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure. (cyberleninka.org)
Researchers can genetically modify Black 6 mice using Black 6 ESCs, or they can use CRISPR/Cas to make knockout mice in just about any background. (the-scientist.com)
We describe all experimental steps from genetically modifying a locus of interest, via CRISPR/Cas9 editing in mouse embryonic stem cells (ESCs), to generating chimeric mice with ESC-reconstituted forebrain regions that can be directly analyzed. (researchsquare.com)
Established in response to a need for in-house mouse services, the LUTCF provides expertise in cryopreservation of embryos by IVF or natural matings, sperm cryopreservation, rederivation services, strain expansion by IVF, ES morula/blastocyst injections, pronuclear DNA microinjections, and injection of CRISPR edited DNA. (lu.se)
An important component of our platform is genome editing by CRISPR-Cas. (lu.se)

The researchers turned to monkeys for more insight into the capabilities of embryonic stem cells. (livescience.com)
Most experiments on stem cell therapies are based on mice, and the researchers wanted to understand whether primate embryonic stem cells respond the same way as those of mice do. (livescience.com)
This is in contrast to pluripotent stem cells, which can differentiate into any tissue type in the body, but not certain embryonic tissues or entire organisms. (livescience.com)
In this procedure, embryonic stem cells are injected into a host embryo after they have been cultured for as long as decades. (livescience.com)
The researchers guessed that the culturing somehow had changed these embryonic stem cells . (livescience.com)
18 Sep, 2007 12:13 pm Rice University researchers have engineered musculoskeletal cartilages with human embryonic stem cells, with the hope of eventually using the neotissue. (scitizen.com)
These cells have been described as embryonic stem cells (ESCs) and. (scitizen.com)
6 Jun, 2007 07:00 pm Researchers have reprogrammed skin cells into embryonic stem cells. (scitizen.com)
Happily, the embryonic stem cell cultures that Martin Evans was then developing provided the necessary vehicle for taking such gene manipulations from the Petri dish into the whole animal. (nobelprize.org)
The North American Conditional Mouse Mutagenesis project (NorCOMM) is a large-scale research initiative focused on developing and distributing a library of mouse embryonic stem (ES) cell lines carrying single conditional knockout mutations across the mouse genome. (tigm.org)
Knockout and other transgenic mice are traditionally made by genetically modifying embryonic stem cells (ESCs) in a dish and then inserting them into a recipient mouse embryo. (the-scientist.com)
NBC involves molecular biology approaches and techniques involving mouse embryonic stem cells and mouse embryos that fall within the technical repertoire of laboratories addressing neurobiological and developmental questions. (researchsquare.com)
Genetically modified mouse strains derived from embryonic stem (ES) cells have become essential tools for functional genomics and biomedical research. (liveconscience.com)

A conditional knockout mouse line, called Cadm1tm1.2Brd was generated. (wikipedia.org)
Conditional deletion of the Bmi-1 polycomb protein depleted pre-GEPCOT and GEPCOT cells, though pre-GEPCOT cells were more dependent upon Bmi-1 for Cdkn2a ( p16 Ink4a ) repression. (elifesciences.org)
A conditional knockout mouse line called Il23r tm2a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute . (wikidoc.org)

Senp2 knockout mice exhibited a lipodystrophy-like phenotype. (deepdyve.com)

These extensive high-throughput data from IMPC mice provide a promising opportunity to explore genetics causing metabolic heart disease with an important translational approach. (springer.com)
The Mouse Genome Informatics (MGI) Database at the Jackson Laboratory provides integrated access to data on the genetics, genomics and biology of the laboratory mouse. (tigm.org)
MTB integrates data on the frequency, incidence, genetics, and pathology of neoplastic disorders, emphasizing data on tumors that develop characteristically in different genetically defined strains of mice. (tigm.org)
Here, we used mouse genetics, mechanical loading, and pharmacological approaches to test the possibility that polycystin-1 and Wwtr1 have interdependent mechanosensing functions in osteoblasts. (bvsalud.org)
People don't realize that recombination occurs very infrequently and that you're going to end up with this chunk of DNA from one strain on the background of the other," said Jake Lusis , who studies mouse genetics and cardiovascular and metabolic disorders at the University of California, Los Angeles, and was not involved in the study. (the-scientist.com)
That's what led me by the nose to genetics-because if you're going to focus on something, the best lens to use initially is human genetics, and from human genetics to IPSC and the genome editing tools that we use today in the lab. (medscape.com)

To create the chimeric monkeys, researchers essentially glued together cells from individual rhesus monkey embryos and then implanting these mixed embryos into mama monkeys. (livescience.com)
The key was mixing cells from very early-stage embryos, or blastocysts, that consisted of just two to four cells - each one of the cells still totipotent, capable of transforming into a whole animal as well as the placenta and other life-sustaining tissues. (livescience.com)
Since 129 strain mice are difficult to breed, researchers typically transfer their ESCs into the embryos of Black 6 mice and then repeatedly backcross the animals with Black 6 mates while selecting for their 129 strain-derived transgene, slowly eliminating 129 strain DNA surrounding the transgene through recombination. (the-scientist.com)
medium (Millipore) almost doubled the effectiveness of M6 Sera cell derivation and generation of germline chimeras and completely Sera cell-derived animals by aggregation with tetraploid embryos [17]. (liveconscience.com)
A variety of sponsor embryos and methods possess been used to generate germline chimeras from M6 Sera cells such as BALB/c and C57BT/6-blastocyst injections. (liveconscience.com)
For these reasons, alternate sponsor embryos for M6 Sera cells have been looked into, C3HBALB/c [28]. (liveconscience.com)
Aggregation of cleavage stage embryos with Sera cells [29] gives an accessible method for generating Sera cell chimeric mice. (liveconscience.com)
This is currently the most popular method for the generation of targeted knock-out and knock-in models by ES-cell injections into 8-cell morulae and/or blastocysts of mouse embryos. (lu.se)

The researchers first tried creating chimeric monkeys using the process for chimeric mice. (livescience.com)
But the methods that work to create chimeric mice failed in rhesus monkeys, leading to offspring with cells only from the host embryo. (livescience.com)
Improved methods for the generation of germline transmitting chimeric mice from M6 Sera cell lines will make the IKMC and additional M6 Sera cell resources more accessible to the broader biomedical Aliskiren hemifumarate study community. (liveconscience.com)

The integration of in vitro cardiac tissue models, human induced pluripotent stem cells (hiPSCs) and genome-editing tools allows for the enhanced interrogation of physiological phenotypes and recapitulation of disease pathologies. (nature.com)
Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)
Our most recent initiative is having established a platform for induced pluripotent stem cells (iPSC). (lu.se)
I fell in love with the idea of human pluripotent stem cells and started surveying the United States and even Europe for who was playing in that area. (medscape.com)

If you were to pluck two cells from a chimeric mouse's body, you could get two different genomes - complete sets of chromosomes and genetic information. (livescience.com)
Causal impact of a full-gene deletion will be investigated in all organ systems by comparing phenotypic characteristics of KO-mice with wild-type control mice of the same genetic C57BL/6N background (Munoz-Fuentes et al. (springer.com)
10 Oct, 2007 12:18 pm The field of functional genomics explores the various functions of genetic sequences within the human genome. (scitizen.com)
Genetically-modified mice have therefore frequently helped to reveal a gene's function and, since mice and humans share a remarkable genetic similarity, they also serve as models of many human diseases. (nobelprize.org)
Mouse SSCs can be maintained under culture conditions, continuing to proliferate for up to 2 years without losing their spermatogenic ability as well as maintaining genetic, epigenetic, and karyotypic stability. (cyberleninka.org)
2004). In order to make GS cells more widely applicable for the study of spermatogenesis at the genetic and molecular levels, it is desirable to be able to modify their genome without disturbing their spermato-genic ability. (cyberleninka.org)
We created and compared the skeletal phenotypes of control Pkd1flox/+;Wwtr1flox/+, Pkd1Oc-cKO, Wwtr1Oc-cKO, and Pkd1/Wwtr1Oc-cKO mice to investigate genetic interactions. (bvsalud.org)
FLICKR, STEFANO Passenger mutations-unwanted alterations that accompany intentional genetic modifications-can confound experiments using certain types of transgenic mice. (the-scientist.com)
Passenger mutations affect experiments using transgenic animals models called congenic mice, whose modified genetic material comes from a different genetic background than the rest of their genome. (the-scientist.com)

Some genetically engineered mice harbor unwanted mutations that hitchhike alongside desired modifications, affecting experimental outcomes. (the-scientist.com)
When researchers compare their modified mice to control Black 6 mice, they may observe phenotypic differences that result from passenger mutations in 129 mouse DNA, rather than from changes to their actual gene of interest, according to the Ghent team. (the-scientist.com)
however, in spite of the obvious advantages of Aliskiren hemifumarate the inbred background few targeted mutations have been published compared to the widely used 129 Sera cell lines [1]. (liveconscience.com)

The International Mouse Strain Resource (IMSR) is a searchable online database of mouse strains and stocks available worldwide, including inbred, mutant, and genetically engineered mice. (tigm.org)
The Ghent researchers looked through the sequences for differences between Black 6 mice and the 129 strains that would affect protein production. (the-scientist.com)
Here, we show that the nuclear genomes of three strains of Anaeramoeba encode between 45 and 103 inteins, in stark contrast to four found in the most intein-rich eukaryotic genome described previously. (lu.se)

The cells never fuse, but they stay together and work together to form tissues and organs," said Shoukhrat Mitalipov of the Oregon National Primate Research Center at Oregon Health & Science University. (livescience.com)
These stem cells will mix with the host embryo's cells to produce tissues and organs and ultimately offspring. (livescience.com)
Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
Feric, N. T. & Radisic, M. Maturing human pluripotent stem cell-derived cardiomyocytes in human engineered cardiac tissues. (nature.com)
Although less fat stor- adqcKO pose lipid storage in adipocyte-speci﫿c Senp2 knockout mice fed age was shown in Senp2 adipose tissues, the increased with high-fat diets (HFD). (deepdyve.com)
27 Jun, 2007 06:08 pm Stem cells have the potential to become all the cells and tissues in the human body. (scitizen.com)
Furthermore, CellMapper makes accurate predictions for human brain cell types that have never been isolated, and can be rapidly applied to diverse cell types from many tissues. (biomedcentral.com)
As a result, expression data are only available for a small fraction of the ~150 cell types [ 7 ] of the human central nervous system and this problem is similar for many other tissues. (biomedcentral.com)
In facial morphogenesis, neural crest cells migrate into the facial region, where they form the skeletal and connective tissue and all dental tissues except the enamel. (medscape.com)

The course will be split into two groups (mammalian cells OR mice) based on participants' expertise. (sb-3.org)
Working against a background of skepticism, they independently discovered that they could use a natural mechanism, revealed decades before by Joshua Lederberg in bacteria, to introduce short sequences of manipulated DNA into the chromosomes of mammalian cells growing in the laboratory. (nobelprize.org)

The Genome of C57BL/6J "Eve", the Mother of the Laboratory Mouse Genome Reference Strain. (jax.org)

Genome-wide association studies identified an association between body mass index and two loci near the CADM1 and CADM2 gene. (wikipedia.org)

Whole-genome sequences were generated for preservation of the primary attenuation site [domain V in the these 251 nOPV2 cell-culture isolates. (cdc.gov)

In this review, we summarize the current advances of the IMPC in cardiac research and describe in detail the diagnostic requirements of high-throughput electrocardiography and transthoracic echocardiography capable of detecting cardiac arrhythmias and cardiomyopathies in mice. (springer.com)

Furthermore, in silico analysis from The Cancer Genome Atlas (TCGA) database suggests that overexpression of ALKBH6 provides better survival outcomes in patients with pancreatic cancer. (frontiersin.org)

She serves on NIH study sections and as reviewer for journals in her area of expertise, manages an active laboratory, supervises students in pulmonary biology and epithelial cell research and has received awards recognizing both teaching and original research accomplishments. (rochester.edu)
Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. (deepdyve.com)
But a substantial quantity of biology research has been built on traditionally constructed transgenic mice, and many labs continue to use them. (the-scientist.com)

All appear male, though testing on their cells reveals that they also contain individual female genomes. (livescience.com)
Resolution of structural variation in diverse mouse genomes reveals chromatin remodeling due to transposable elements. (jax.org)

Until recently, these cells almost always came from 129 strain mice, a group of substrains of mice that produce ESCs that are unusually robust and easy to manipulate. (the-scientist.com)

Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. (cancerindex.org)
During cell growth and proliferation, ubiquitin plays an outsized role in promoting progression through the cell cycle. (intechopen.com)
Notably, the G1/S boundary represents a major barrier to cell proliferation and is universally dysfunctional in cancer cells, allowing for the unbridled proliferation observed in malignancy. (intechopen.com)
Autophagy also regulates cell proliferation, differentiation, survival, and apoptosis, highlighting its role in maintaining cellular homeostasis [ 1 ]. (hindawi.com)

Consequently, neural stem and progenitor cells have usually been studied retrospectively, based on their ability to form colonies in laboratory cell cultures. (elifesciences.org)
Knock-out (and knock-in) mice, the workhorses of many a laboratory today, allow researchers to study the effects of removing (or inserting) a single gene. (nobelprize.org)
The Jackson Laboratory , offers a fully integrated suite of services to provide efficient and cost-effective solutions to cryopreserve, expand, breed and characterize your mice following recovery from the TIGM library. (tigm.org)

Type 1 diabetes results from the poorly understood process of islet autoimmunity, which ultimately leads to the loss of functional pancreatic beta cells. (springer.com)
How the DNA encoding these selfish elements spreads within and between genomes is poorly understood, particularly in eukaryotes where inteins are scarce. (lu.se)

Results Conditioned Sera cell medium significantly enhances chimera production from M6-produced C2 Sera cells Blastocyst injection is definitely the most common method for. (liveconscience.com)

Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)
We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)

Twenty six tests were carried out on homozygous mutant mice and one significant abnormality was observed: males were infertile. (wikipedia.org)
Moreover, FPC inactivation significantly exacerbates renal cystogenesis and triggers severe pancreatic cystogenesis in a Pkd1 mouse mutant Pkd1V/V in which cleavage of Pkd1-encoded Polycystin-1 at the GPCR Proteolysis Site is blocked. (bvsalud.org)

We procure skin biopsies from patients, reprogram fibroblasts from these into stem cells, i.e., iPSC, which are then differentiated into insulin-producing beta-like cells. (lu.se)
Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)

That's because, according to a 1995 study and subsequent work, Casp1 knockout mice did not go into septic shock when challenged with molecules signaling foreign invaders. (the-scientist.com)

Here, we tested whether ALKBH6 plays a significant role in preventing alkylating DNA damage and decreasing genomic instability in pancreatic cancer cells. (frontiersin.org)
In particular, the loss of ALKBH6 in human pancreatic cancer cells increases alkylating agent-induced DNA damage and significantly decreases cell survival. (frontiersin.org)
Overall, our data suggest that ALKBH6 is required to maintain the integrity of the genome and promote cell survival of pancreatic cancer cells. (frontiersin.org)
The extremely low 5-year overall survival of patients is, in part, due to pancreatic cancer cells having several mechanisms of resistance to different chemotherapeutic treatments, one of which is their capacity to efficiently repair alkylating agent-induced DNA damage. (frontiersin.org)

The ideal result is an almost entirely Black 6 mouse with a 129 strain transgene. (the-scientist.com)
Specifically, 129 strain congenic mice on a Black 6 background that have been backcrossed for 10 generations have more than a 90 percent chance of having around 1 million base pairs of 129 strain DNA on either side of the transgene. (the-scientist.com)
However, in 2011, researchers at Genentech showed that many Casp1 knockout mice also harbored a mutated Casp11 gene from 129 strain mice. (the-scientist.com)

Here, we review some of the recent advances and studies that highlight the roles of these changes as well as antigen presentation and stress response pathways in beta cells in the onset and propagation of the autoimmune process in type 1 diabetes. (springer.com)
Researchers can search and browse the IGTC database for cell lines of interest using accession numbers or IDs, keywords, sequence data, tissue expression profiles and biological pathways. (tigm.org)

Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. (cyberleninka.org)
The cultured SSCs, named germline stem (GS) cells, are now useful in research on various aspects of spermatogenesis. (cyberleninka.org)
A recent statement with a large quantity of clones showed only about 43% 81% of germline transmitting HSPA1 clones for M6 129S5 Sera cells [14]. (liveconscience.com)
Invitrogen) also facilitated the derivation of karyotypically stable and germline proficient M6 Sera cells [18]. (liveconscience.com)
Here, we describe a KOSR-based medium for the efficient generation of germline chimeras using our M6-produced Sera cell collection, designated C2, and its targeted subclones. (liveconscience.com)
Here, we display that ICR morula aggregation is definitely also an efficient method for generating germline transmitting chimeras from M6In Sera cell lines. (liveconscience.com)
Although good quality ES cells can provide chimeras, we do not guarantee germline transmission. (lu.se)

Neurosphere formation is commonly used as a surrogate for neural stem cell (NSC) function but the relationship between neurosphere-initiating cells (NICs) and NSCs remains unclear. (elifesciences.org)
Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)
Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes. (nature.com)
27 Nov, 2007 10:41 am Tony Maciulis talks with Dr. Jon LaPook about a breakthrough in stem cell research. (scitizen.com)
Ethical Stem Cell Breakthrough! (scitizen.com)
21 Nov, 2007 10:54 am A huge advancement in stem cell research--and a stake in the heart of human cloning--was announced yesterday. (scitizen.com)
This new technique could be used to develop stem cell lines without use of controversial. (scitizen.com)
We're thrilled to have Chad Cowan, an associate professor at Harvard University who is at the Harvard Stem Cell Institute. (medscape.com)
At International Society for Stem Cell Research-the big stem cell meeting-Yamanaka revealed one more factor. (medscape.com)

So they recovered stem cells from an embryo's inner cell mass (rather than from the freezer after being cultured) and, without culturing them, injected the stem cells into a host embryo. (livescience.com)
EmbryoMax KSOM mouse embryo medium (Millipore, cat. (researchsquare.com)

Since the early 1990s we have specifically used the aggregation method to generate multiple genetically revised mouse stresses from 129S1129X1 (L1) [30] and 129S6B6F1 (G4) [31] Sera cells. (liveconscience.com)

8 Jun, 2007 04:13 pm Stem cells provide the starting material for the development and repair of every organ and tissue in the body and they are present in all stages of life. (scitizen.com)
The human brain provides a clear example: many brain cell types display abnormal gene expression patterns when grown in culture [ 2 ] and must be acutely isolated from intact brain tissue to insure physiological relevance. (biomedcentral.com)
One promising solution has been the development of computational methods to infer cell type-specific expression information directly from heterogeneous samples [ 8 - 19 ], such as undissociated tissue. (biomedcentral.com)
To contribute to the hazard identification of low molecular weight (LMW) respiratory allergens, respiratory allergy induced by trimellitic anhydride (TMA) was characterized by whole genome analysis of lung tissue and blood proteomics in Brown Norway rats. (cdc.gov)

Further analysis showed spermatogenesis had arrested in these mice. (wikipedia.org)
Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
Micro-CT 3D image analysis indicated that the reduction in bone mass was due to greater loss in both trabecular bone volume and cortical bone thickness in Pkd1/Wwtr1Oc-cKO mice compared to either Pkd1Oc-cKO or Wwtr1Oc-cKO mice. (bvsalud.org)
Furthermore microarray analysis of gene appearance patterns in cultured 129- and M6-produced Sera cells showed that M6 Sera cells have a higher inclination to shed their pluripotency in tradition in standard 15% FBS LIF-supplemented Sera cell medium than 129 Sera cells [16]. (liveconscience.com)

At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide. (ubigene.us)
For gene-editing mice. (ubigene.us)
No previous experience in genome editing is required. (sb-3.org)
Brietta Latham, a PhD student in WSU's School of Molecular Biosciences, stands next to a tank containing beetles and caterpillars that clean mouse skeletons used in research to develop a gene editing strategy to shorten the tails of Suffolk sheep and eliminate the need for tail docking. (wsu.edu)

And the single-cell clones were isolated by the limited dilution method and the Monoclone Genotype Validation Kit (Cat: YK-MV-1000) was used for cell lysis and PCR amplification on the single-cell clones, and then the genotype was verified by Sanger sequencing. (ubigene.us)

Today, genetically-altered mice are an essential component of the experimental toolkit, with thousands of varieties contributing to research in laboratories around the world. (nobelprize.org)

Autophagy is an intracellular degradation system that ensures a dynamic recycling of a variety of building blocks required for self-renewal, homeostasis, and cell survival under stress. (hindawi.com)
Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)

We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)

In particular, ubiquitin-mediated degradation is critically important at transition points where it provides directionality and irreversibility to the cell cycle, which is essential for maintaining genome integrity. (intechopen.com)

Osteoclasts are bone cells derived from monocyte/macrophage lineage and are exclusively responsible for bone resorption, which contributes to skeletal development, bone homeostasis, and remodeling. (frontiersin.org)

Particularly, viral infection triggers specific signaling programs within the infected cells that results in substantial changes in host gene expression. (cdc.gov)
GXD integrates different types of gene expression information from the mouse and provides a searchable index of published experiments on endogenous gene expression during development. (tigm.org)
Pkd1/Wwtr1Oc-cKO mice also displayed additive reductions in mechanosensing and osteogenic gene expression profiles in bone compared to Pkd1Oc-cKO or Wwtr1Oc-cKO mice. (bvsalud.org)

Cell adhesion molecule 1 is a protein that, in humans, is encoded by the CADM1 gene. (wikipedia.org)
Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)
We found that SET domain bifurcated 1 Since no difference in food intake was observed between adqcKO f/f (Setdb1) was a SUMOylated protein and that Senp2 de-SUMOylated Senp2 and Senp2 mice fedeitherwithNCD or HFD and regulated Setdb1 action in trimethylation at histone 3 lysine 9 (Supplementary Figure S2A). (deepdyve.com)
In the case of G M2 gangliosidosis, an activator protein is deficient, which leads to the accumulation of ganglioside G M2 and related glycolipids in neuronal cells. (medscape.com)

We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)

Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)

Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)
Neural stem cells have long lives, much of which they spend in a quiescent state. (elifesciences.org)
A region of the brain called the subventricular zone contains both neural stem cells and neural progenitor cells, and is one of only two regions of the brain where neural stem cells are found in adult mammals. (elifesciences.org)
However, the exact relationship between neural stem cells and neurosphere-forming cells has been uncertain. (elifesciences.org)
have used a technique called flow cytometry to identify and isolate neural stem cells and neurosphere-forming cells directly from the subventricular zone. (elifesciences.org)
The neural stem cells, which Mich, Signer et al. (elifesciences.org)
To circumvent these obstacles, we have developed two methods for the investigation of human neural cells in culture. (lu.se)

Anatomy10

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Phenomena and Processes51

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International Mouse Phenotyping Consortium3

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