Mouse carotid arteries. Medical search. Frequent questions

Hypoxic-ischemic brain injury (HI) was induced by unilateral carotid artery ligation and subsequent exposure to 10% oxygen for 50 minutes in infant (P9) and juvenile (P30) mice. (wisc.edu)
Hypoxia-ischemia (HI) was induced in P9 and P30 mice using unilateral carotid artery ligation and exposure to 10% oxygen for 50min. (wisc.edu)
At the end of the blocking incubation, coincubate the cells with primary mouse antibody against ubiquitin-specific peptidase 8, primary rabbit antibody against neuregulin receptor degradation protein-1, and a marker specific for beta cell identification that was not raised in mouse or rabbit so as not to interfere with the proximity ligation assay signal overnight at four degrees Celsius. (jove.com)
Co-registered 3D rendered ultrasound (greyscale) and color Doppler (red and blue) coronal image of a mouse brain during ligation of the right common carotid artery. (visualsonics.com)
Our group has shown that TGF-b1/ALK1 signalling drives SMC migration and that NF in ligation-injured carotid arteries is blunted in heterozygous Smad1 knockout mice. (gla.ac.uk)
Together, these results prompted an observational multiple timepoint carotid artery ligation study in mice which aimed to link BMP-9 presence with vascular injury responses. (gla.ac.uk)
Carotid ligation triggered intimal and medial SMC proliferation which was paralleled by a loss of SMC a-smooth muscle actin (SMA) indicating SMC phenotype switching. (gla.ac.uk)
Single-cell RNA sequencing (scRNAseq) and scATAC sequencing (scATACseq) study using the partial carotid ligation mouse model showed flow-regulated Klk10 expression at the epigenomic and transcription levels. (cdc.gov)

Then use the microneedle holder to dissect the infrarenal aorta and IVC en bloc from the lateral and dorsal surrounding retroperitoneal tissues to cross-clamp the tissues together, and dissect the surrounding tissues to expose the aortic puncture site at approximately three-quarters of the distance from the left renal vein to the aortic bifurcation. (jove.com)
The animals were sacrificed and proceeded to collect tissues of arteries (aorta, carotid and femoral arteries), heart, kidneys and lungs for analysis of the synthesis and degradation of ECM components. (usp.br)
Upper right: Slightly more dorsal (posterior) frontal view showing the main pulmonary artery (MPA), large left-sided patent ductus arteriosus (PDA), and left-sided upper descending aorta (DescAo). (medscape.com)

Kelly, S, McCulloch, J & Horsburgh, K 2001, ' Minimal ischaemic neuronal damage and HSP70 expression in MF1 strain mice following bilateral common carotid artery occlusion ', Brain Research , vol. 914, pp. 185 - 195. (bris.ac.uk)
Middle Cerebral Artery Occlusion Allowing Reperfusion via Common Carotid Artery Repair in Mice JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. (nottingham.ac.uk)
A study by Gill et al indicated that greater genetically determined levels of factor XI increase the risk of ischemic stroke arising from cardioembolisms, while not impacting ischemic stroke risk associated with large artery atherosclerosis or small artery occlusion. (medscape.com)
Researchers used a mechanism-based therapeutic approach for stroke first to examine the connection of mitochondrial, autophagy and ER stress inhibition in the protective action of GCSF and then to analyze relevant ER stress pathways in the bilateral common carotid artery occlusion (BCAO) model of stroke. (sciencedaily.com)
Expression of SOX9 did not decrease during aging and was instead upregulated by reactive astrocytes in a number of settings, including a murine model of amyotrophic lateral sclerosis (SOD1G93A), middle cerebral artery occlusion, and multiple mini-strokes. (jneurosci.org)

Previously, we identified kallikrein-related peptidase 10 (Klk10, a secreted serine protease) as a flow-sensitive gene in mouse arterial ECs, but its role in endothelial biology and atherosclerosis was unknown. (cdc.gov)
Additionally, rKLK10 injection or ultrasound-mediated transfection of Klk10-expressing plasmids inhibited atherosclerosis in Apoe(-/-) mice. (cdc.gov)

RESULTS: Between July 2003 and November 2006, 92 patients had a single internal carotid artery treated, while 7 underwent staged bilateral CAS. (uzh.ch)

Inclusion criteria included recent (within 30 days of the date of procedure) CTA of the head and neck, evidence of an carotid stenosis of more than 70% confirmed with conventional angiography or severe intracranial major artery stenosis with distinct low perfusion of the relevant regions, age older than 45 years, and selected by the vascular disease specialist as a suitable candidate for stent placement or EC-IC by-pass. (scirp.org)
Controls were patients with carotid or/and intracranial severe stenosis who declined vascular surgery due to personal reasons or no surgery was possible due to medical reasons. (scirp.org)
Basal NO release following carotid injury was assessed by N(G)-nitro-L-arginine methyl ester-induced vascular contraction. (ox.ac.uk)
BALB/c aortas (H-2(d)) transplanted into α-TFPI-transgenic (Tg) mice (H-2(b)) regenerated a quiescent endothelium in contrast to progressive IH seen in C57BL/6 wild-type (WT) mice even though both developed aggressive anti-H-2(d) alloresponses, indicating similar vascular injuries. (nih.gov)
In addition, carotid artery wire injury in mice triggered a trend towards an increase in total vascular BMP-9 levels, which was paralleled by SMC phenotype switching, indicating a potential role for BMP-9 in NF. (gla.ac.uk)
Functionally, KLK10 protected against d-flow-induced permeability dysfunction and inflammation in human artery ECs, as determined by NFB activation, expression of vascular cell adhesion molecule 1 and intracellular adhesion molecule 1, and monocyte adhesion. (cdc.gov)

Background: To compare the neuropsychological consequences after carotid artery stenting (CAS) and extracranial-intracranial by-pass (EC-IC by-pass). (scirp.org)
Liu, L. and Zhou, Y. (2015) Effect of Carotid Artery Stenting and Extracranial-Intracranial By-Pass on Cognitive Function: Preliminary Results. (scirp.org)
To reduce the risk of stroke, carotid artery stenting (CAS) is performed and has shown to be effective in preventing from stroke in patients with severe carotid artery stenosis. (scirp.org)
BACKGROUND: Little is known on the performance of newly initiated carotid artery stenting (CAS) programs. (uzh.ch)
Impact of Carotid Stent Design on Embolic Filter Debris Load During Carotid Artery Stenting. (medscape.com)

Arachnoid membrane as a source of sphingosine-1-phosphate that regulates mouse middle cerebral artery tone. (nih.gov)
Intracranial Vessel Wall Magnetic Resonance Imaging of Middle Cerebral Artery Dissection in Neurofibromatosis Type 1. (medscape.com)

Coronary artery bypass grafting (CABG) is a surgical technique utilised to bypass one or more stenosed coronary arteries to re-establish blood supply to cardiac tissue. (gla.ac.uk)
Moreover, KLK10 expression was significantly reduced in human coronary arteries with advanced atherosclerotic plaques compared to those with less severe plaques. (cdc.gov)

2. Transluminal wire injury was induced in the left carotid arteries of adult male wild-type C57BL/6 (WT) and endothelial GTPCH transgenic (Tg-GCH) mice. (ox.ac.uk)
The study was done in vivo in C57Bl/6J inbred mice undergoing treatment for 7 and 14 days subpressor doses of Ang II, AT1 receptor blocker (Losartan) and a combination thereof. (usp.br)
Methods: ICH was induced by collagenase injection into the striatum of wild type (C57BL/6J) anticoagulated mice (warfarin or rivaroxaban) and Mmp10 -/- mice. (unav.edu)

Both arterial GTPCH enzyme activity and BH(4) levels were significantly elevated in Tg-GCH mice compared with WT mice and basal NO release of the injured carotid artery tended to increase in Tg-GCH mice. (ox.ac.uk)
This model is most commonly used to measure arterial pressure via the left carotid artery. (datasci.com)
The most problematic complication of PCI, with or without stent implantation, is the restenosis defined as the re-narrowing of the enlarged artery and characterized by an immune/inflammatory response going with a hyperplastic reaction, involving smooth muscle cell (SMC) migration/proliferation, and remodelling of the arterial wall. (unina.it)
In this study, we investigated the effect of NBD peptide on neointimal formation using two animal models of arterial injury: rat carotid artery balloon angioplasty and wire-induced carotid injury in apolipoproteinE-deficient (apoE-/-) mice. (unina.it)
Furthermore, treatment of mice in vivo with rKLK10 decreased arterial endothelial inflammation in d-flow regions. (cdc.gov)

Conclusions: CAS and EC-IC by-pass in patients with a carotid or intracranial stenosis may result in cognitive improvement three months after surgery. (scirp.org)
The degree of carotid and intracranial stenosis was initially evaluated by CT angiogram (CTA), and conventional cerebral angiography was followed in anticipation of CAS and EC-IC by-pass. (scirp.org)
CAS was performed if the patients had severe symptomatic carotid stenosis ≥70% without severe diffused intracranial artery stenosis. (scirp.org)
Should patients with asymptomatic carotid artery stenosis undergo carotid procedures, or does medical therapy provide sufficient protection against stroke? (medscape.com)

Adoptively transferred Tg CD34(+) (but not CD34(-)) cells inhibited IH in WT recipients, indicating the phenotype of α-TFPI-Tg mice was due to these cells. (nih.gov)
To identify a nuclear marker pathognomonic of astrocytic phenotype, we assessed differential RNA expression by FACS-purified adult astrocytes and, on that basis, evaluated the expression of the transcription factor SOX9 in both mouse and human brain. (jneurosci.org)
The observation of in vivo SMC phenotype switching in pre-implantation SVGs prompted development and generation of a ligand-independent contractile differentiation protocol for primary HSVSMCs and primary human coronary artery (HCA) SMCs. (gla.ac.uk)
[ 1 ] A recent hypothesis to explain the migraine-stroke association, based on experimental data obtained in mice expressing familial hemiplegic migraine type 1 mutations, is that the cerebral hyperexcitability phenotype associated with migraine might sensitize brain tissue to ischemia. (medscape.com)

The mouse model of cerebral artery ischemia/reperfusion injury was established to test the anticerebral ischemia-reperfusion injury activity of the puerarin derivatives. (hindawi.com)
The results showed that compound 5 significantly prolonged the survival time of mice subjected to acute cerebral ischaemia at all doses tested and exhibited potent neuroprotective activity. (scielo.org.za)
More than 87 percent are ischemic strokes, caused by obstruction of one or more cerebral arteries. (sciencedaily.com)
3D rendered photoacoustic image showing cerebral blood signal in the mouse brain. (visualsonics.com)

Immunohistochemistry (IHC) demonstrated that BMP-9 was present in human pre-implantation SVGs and murine carotid arteries. (gla.ac.uk)

The Prospect T1 is an innovative high-frequency ultrasound system designed specifically for in vivo preclinical imaging in small animals such as mice and rats. (scintica.com)
Here, we show that KLK10 is upregulated under s-flow conditions and downregulated under d-flow conditions using in vivo mouse models and in vitro studies with cultured ECs. (cdc.gov)

The present study tested the hypothesis that endothelial-targeted GTPCH overexpression retards neointimal formation, a hallmark of restenosis, in mouse carotid artery. (ox.ac.uk)
Morphological analysis was performed 2 and 4 weeks after carotid injury to assess neointimal formation. (ox.ac.uk)
Morphological analysis revealed that the endothelial-specific overexpression of GTPCH reduced neointimal formation and medial hypertrophy 2 and 4 weeks after carotid injury. (ox.ac.uk)
Similar results were observed in apoE-/- mice in which bindarit administration resulted in a 42% reduction of the number of proliferating cells at day 7 after carotid injury and in a 47% inhibition of neointimal formation at day 28. (unina.it)

Using a mouse model, researchers investigated the efficacy of GCSF beyond the typical four-hour thrombolytic therapy (tPA) clot-busting drug -- the gold standard to treat stroke for global ischemia. (sciencedaily.com)

An alternative surgical approach reduces variability following filament induction of experimental stroke in mice Disease Models & Mechanisms. (nottingham.ac.uk)
They confirmed the neuroprotection of GCSF gene therapy in the BCAO mouse stroke model by a decrease of dynamin-related protein (DRP1), a marker of mitochondrial stress, in the frontal and middle brain of the GCSF treated group. (sciencedaily.com)

In mice, knockout of the gene for factor XI does not lead to death in utero. (medscape.com)

Endothelium-targeted transgenic GTP-cyclohydrolase I overexpression inhibits neointima formation in mouse carotid artery. (ox.ac.uk)
Endothelium-dependent relaxation was impaired 10 days and 4 weeks after carotid injury, whereas endothelium-independent relaxation remained unaffected. (ox.ac.uk)

These effects were associated with a significant reduction of NF-κB activation and monocyte chemotactic protein-1 (MCP-1) expression in the carotid arteries of rats treated with the peptide. (unina.it)
These effects were associated with a significant reduction of MCP-1 levels both in sera and in injured carotid arteries of rats treated with bindarit. (unina.it)

This study was carried out in 6 and 12 months old transgenic PLP-α-syn and WT male and female mice. (frontiersin.org)

The effect of CM-352 could be related to MMP-10 inhibition since Mmp10 -/- mice showed lower hemorrhage volume, better neurological score, reduced IL-6 levels and neutrophil infiltration, and increased PAI-1 after experimental ICH. (unav.edu)

The assays of the water maze, Y maze, brain cortex Ca 2+ -Mg 2+ -ATP enzyme, and iNOS enzyme activity were performed in this mouse model. (hindawi.com)
[ 7 ] In a mouse model, factor XI appears to play a greater role in thrombosis than in hemostasis. (medscape.com)
GCSF improved long-term behavioral outcomes while also stimulating a neural progenitor recovery response in a mouse model. (sciencedaily.com)

Non-linear contrast bolus perfusion into a subcutaneous tumor in a mouse. (visualsonics.com)

Analysis of the cellular composition in neointimal lesions of apoE-/- mice treated with bindarit showed that the relative content of macrophages and the number of SMCs were reduced by 66% and 30%, respectively, compared with the control group. (unina.it)

The injection of BC cells in the carotid artery of mice has been proposed but the procedure is challenging, with the metastatic pattern being scarcely characterized. (mtak.hu)
With our surgical procedure, both mouse death and procedure-associated weight loss were negligible. (mtak.hu)
Before beginning the procedure, confirm a lack of response to toe pinch in a nine-to 11-week-old, C57-Black/6 mouse and place the mouse in the supine position in the surgical area. (jove.com)
Percutaneous coronary intervention (PCI) is the current procedure that allows the endovascular treatment of occlusive artery disease, without the need of bypass surgery. (unina.it)

High-resolution ultrasound (greyscale) and photoacoustic (red, white and blue) image of a long axis view of the left ventricle of the mouse heart. (visualsonics.com)
In type A interrupted left aortic arch, the arch interruption occurs distal to the origin of the left subclavian artery. (medscape.com)
the two most common abnormal sites are distal to the left subclavian artery (aberrant right subclavian artery) and from a right ductus arteriosus (isolated right subclavian artery). (medscape.com)
Suprasternal echocardiographic identification of interrupted aortic arch Type B. Upper left: Frontal (coronal) view showing the takeoffs of the innominate artery (InnA) and left common carotid artery (LCCA). (medscape.com)

For clamp and puncture site exposure, place the mouse under a dissecting microscope and, using sterile technique and a scalpel, make a skin-deep, midline, abdominal incision from the lower liver edge to just above the pubis. (jove.com)

Triple-negative BC (4T1) cells were inoculated in the common carotid artery of BALB/c mice. (mtak.hu)
For example, 90 families of miRNAs have been conserved since at least the common ancestor of mammals and fish, and most of these conserved miRNAs have important functions, as shown by studies in which genes for one or more members of a family have been knocked out in mice. (wikipedia.org)

PLP-α-syn mice have been shown to recapitulate several features of MSA such as motor dysfunction/parkinsonism ( 17 , 18 ), respiratory dysfunction ( 20 ) and dysautonomia ( 21 ). (frontiersin.org)
Pharmacological evaluation showed pterostilbene could ameliorate cognitive dysfunction, regulate glycolipid metabolism disorders, improve neuronal damage, and reduce the accumulation of β-amyloid in DCI mice. (qualitycounts.com)

Action of angiotensin II in perivascular extracellular matrix remodeling in mice. (usp.br)

Mice overexpressing human α-synuclein under the control of the oligodendrocyte specific proteolipid promoter (PLP-α-syn) display aged-dependent progressive neurodegeneration and have been extensively used in the past to assess MSA-related alterations ( 16 - 19 ). (frontiersin.org)
The goal of this study was to use mice expressing human tissue factor pathway inhibitor (TFPI) on α-smooth muscle actin (α-SMA)(+) cells as recipients of allogeneic aortas to gain insights into the cellular mechanisms of intimal hyperplasia (IH). (nih.gov)
[ 9 ] Interrupted aortic arch is the first cardiovascular pattern formation anomaly to be demonstrated to have a genetic basis in both mouse and human. (medscape.com)

Calcified Basilar Artery Embolus With False-Patency Sign on Computed Tomography Angiogram. (medscape.com)

Consecutive patients who were referred to neuroendovascular unit for carotid artery stent placement or EC-IC by-pass from January 2010 to October 2011 participated in the study. (scirp.org)
These results indicate that MED1 is a positive regulator for hepatic fibrogenesis, and MED1 may be considered as a potential therapeutic target for the regression of liver fibrosis.NEW & NOTEWORTHY In this study, we present the first evidence that liver mediator 1 (MED1) deficiency attenuated carbon tetrachloride-induced hepatic fibrosis in mouse. (bvsalud.org)

An impaired HRV was observed at 12 months of age in PLP-α-syn female but not in male mice, indicative of cardiovascular AF. (frontiersin.org)
Methods: CRP deficient and wild type (WT) male mice were subjected to AAA induction via transient intra-aortic infusion of porcine pancreatic elastase. (bvsalud.org)

In P9 mice, an increase in soma size is seen in surface render images (c,d,i,j) of ipsilateral brain regions, along with a concurrent decrease in hull volume (e,f,k,l). (wisc.edu)
HI resulted in a significant decrease in branching morphology only in the P9 mice, and expression of TGF-β receptor, Serpine1, MerTK, and SOCS3 were elevated in P30 mice compared to P9 post-HI. (wisc.edu)

The Jackson Laboratory offers several hands-on workshops for those wishing to obtain training in surgical techniques for the laboratory mouse. (jax.org)
This four-day intensive workshop is for those wishing to obtain practical hands-on training in mouse surgical techniques. (jax.org)
Participants completing this course will obtain valuable skills in multiple high-demand mouse surgical techniques. (jax.org)

Here, we showed that MED1 expression was increased in livers from nonalcoholic steatohepatitis (NASH) patients and mice and positively correlated with transforming growth factor ß (TGF-ß) signaling and profibrotic factors. (bvsalud.org)

Microglia were isolated from P2, P9, P30 and P60 mice, and expression of markers of classical and alternative microglial activation was assessed, as well as transforming growth factor beta (TGF-β) receptor, Serpine1, Mer Tyrosine Kinase (MerTK), and the suppressor of cytokine signaling (SOCS3). (wisc.edu)
TGF-ß/Smad2/3 signaling pathway was inhibited, and gene expression of fibrotic markers, including α-smooth muscle actin (α-SMA), collagen type 1 α 1 (Col1a1), matrix metalloproteinase-2 (Mmp2), and metallopeptidase inhibitor 1 (Timp1) were decreased in livers of MED1ΔLiv mice with CCl4 injection. (bvsalud.org)

Representative Imaris-processed images are shown in A and B from contralateral (CL) and ipsilateral (IL) hippocampus of P9 and P30 mice at 2 days post-HI. (wisc.edu)

Upon treatment with carbon tetrachloride (CCl4), hepatic fibrosis was much less in liver-specific MED1 deletion (MED1ΔLiv) mice than in MED1fl/fl littermates. (bvsalud.org)

mouse muscle cells. (abcam.com)
WT CD34(+) cells incubated with a PAR-1 antagonist or taken from PAR-1-deficient mice inhibited IH as Tg cells did. (nih.gov)
Inside the body, the junctions between the cells that make up our arteries, veins, and capillaries form a barrier that keeps our blood flowing through our vessels rather than seeping everywhere. (harvard.edu)

It is known that neurons and glia express the putative SARS-CoV-2 receptor angiotensin converting enzyme 2 ( 7 ), and that the related coronavirus SARS-CoV (responsible for the 2003 SARS outbreak) can inoculate the mouse olfactory bulb ( 8 ). (cdc.gov)

Anatomy60

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