Mouse antibody. Medical search. Frequent questions

Cross-reactivity of the polyclonal secondary antibody ab182022 was tested using a sandwich ELISA approach. (abcam.com)
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species. (abcam.com)
In Western blots, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6, recombinant rat EphB1, and rmEphB3 is observed. (rndsystems.com)
This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG 2a , IgG 2b , IgG 3 , and IgA, pooled human sera, and purified human paraproteins. (licor.com)
NKp30 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. (prospecbio.com)
Aydintug K, Inzana TJ, Letonja T, Davis WC, Corbeil LB. Cross-reactivity of monoclonal antibodies to Escherichia coli J5 with heterologous gram-negative bacteria and extracted lipopolysaccharides. (linscottsdirectory.com)
The antibody has been shown to recognize α-skeletal, α-cardiac, α-smooth muscle, β-cytoplasmic, γ-smooth muscle and γ- cytoplasmic actin isotypes (Fig. 1) and has broad species cross-reactivity. (cytoskeleton.com)

Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection. (licor.com)
A panel of 11 murine monoclonal antibodies directed against dengue type 2 was evaluated for antigen specificity by dot immunobinding assay and Western blot analysis and for in vitro and in vivo biological activities. (ajtmh.org)
In this study we have profiled a panel of mouse antihuman CD40 monoclonal antibodies (MoAbs), regarding their CD40 binding affinity and epitope-specificity relative to the CD40L binding in relation to their cellular activating potential. (lu.se)

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. (abcam.com)
Based on ELISA and flow cytometry, the antibody reacts with the heavy chain of mouse IgG 1 . (licor.com)
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab') 2 /Fab portion of mouse IgG. (jacksonimmuno.com)

The details of the immunogen for this antibody are not available. (abcam.com)
Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. (abcam.com)
Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. (biocheminfo.org)
The immunogen used for antibody production was purified actin protein from rabbit skeletal muscle. (cytoskeleton.com)

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram). (stemcell.com)
Enriched BALB/c mouse splenic T cells were stimulated with PMA plus ionomycin for 6 hours and then stained with mouse CD154 (CD40L) (clone SA047C3) FITC (flled histogram) or rat IgG2a FITC isotype control (open histogram). (biolegend.com)
Search, find, compare suppliers for Mouse IgG3 Isotype Control products. (linscottsdirectory.com)

Antibodies are proteins which are naturally formed by the body in response to a foreign substance, known as an antigen. (wikipedia.org)
FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. (jacksonimmuno.com)
No antibody was detected against non-immunoglobulin serum proteins. (abcam.com)
No antibody was detected against the Fc portion of mouse IgG or against non-immunoglobulin serum proteins. (jacksonimmuno.com)
Absolute Antibody has created mouse bispecific antibodies to target key proteins involved in different immunological pathways, including PD-L1, CD3ε and CD47. (nepic.co.uk)

These types of antibodies are typically called monoclonal antibodies because they are created to target one specific antigen. (wikipedia.org)
FOLR1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Mouse FOLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6936) at 10 µg/mL for 3 hours at room temperature. (rndsystems.com)
avenue of research has been the development of protein-based "point-of-care" diagnostic assays for antigen and antibody detection. (who.int)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

PUS1 antibody LS-C30189 is an unconjugated rabbit polyclonal antibody to mouse PUS1 (C-Terminus). (lsbio.com)
Affinity purified polyclonal antibody to mouse IgG and IgM heavy and light chains (whole IgA, IgG, IgM) made in goat and biotinylated. (seracare.com)

This antibody may cross react with IgG from other species. (abcam.com)
This antibody is recommended for detection of actin in a broad spectrum of species such as human, mouse, rat, and bovine. (cytoskeleton.com)
Of the four antibodies tested, only aducanumab dramatically reduces the flux of amyloid-β oligomers, a key neurotoxic species associated with AD. (lu.se)

Human anti-mouse antibody or human anti-murine antibody (HAMA) is an antibody found in humans which reacts to immunoglobins found in mice. (wikipedia.org)
Bispecific antibodies, which permit simultaneous engagement of two different protein targets, are an exciting new class of therapeutics, but many of the formats developed for human antibodies do not readily transfer to murine antibodies. (nepic.co.uk)
These new murine bispecific reagents enable researchers and drug developers to more easily evaluate potential bispecific combinations in mouse models. (nepic.co.uk)
Absolute Antibody's new bispecific antibodies are fully murine, meaning they can be used in vivo without rapidly inducing neutralizing, anti-drug antibodies. (nepic.co.uk)
In 2018, the company launched its first-generation murine bispecific antibodies in the IgG:dAb format, in a world-first for the reagents market. (nepic.co.uk)
However, murine models, including knockout and transgenic mice, have been useful in understanding the mechanisms of B lymphopoiesis, B-cell differentiation, and antibody formation. (medscape.com)
Murine gene mutations in human counterparts may be associated with a clinical illness different from the illness seen in mice. (medscape.com)

The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. (biolegend.com)
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) FITC (solid line), or rat IgG2a, κ FITC (broken line). (biolegend.com)

The first of these is that they note that some anti-Zika antibodies cross-react with Dengue virus. (sciencemediacentre.org)

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852) and EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit (Catalog #18782). (stemcell.com)
Absolute Antibody is committed to creating a unique reagents catalog through recombinant protein engineering approaches," said Dr. Michael Fiebig, Director of Products and Innovations at Absolute Antibody. (nepic.co.uk)
Absolute Antibody has grown rapidly since its founding in 2012, offering antibody sequencing, engineering and expression as custom services, as well as a catalog of more than 3,800 engineered recombinant antibodies. (nepic.co.uk)

The antibody reacts with mouse immunoglobulins of all classes. (abcam.com)

Absolute Antibody, an industry-leading provider of recombinant antibody products and services, today announced the launch of the first commercially available mouse knob-into-hole (KIH) bispecific antibody reagents. (nepic.co.uk)

IRDye 680LT Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. (licor.com)
Computer artwork of an antibody or immunoglobulin molecule attacking a leukaemia white blood cell. (lu.se)

IgG fraction Monoclonal Mouse Anti-Digoxin may be used either as direct conjugates, or for more sensitivity, they can be used unconjugated followed by a conjugated anti-mouse IgG (H+L) for signal enhancement . (jacksonimmuno.com)
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. (biotium.com)
Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. (biotium.com)
For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates). (biotium.com)

IRDye 680LT Goat anti-Mouse IgG1-Specific Secondary Antibody. (licor.com)
Mouse IgG1 heavy chain and κ light chain. (prospecbio.com)

We modified a previously described protein-A/G horseradish peroxidase enzyme-linked immunosorbent assay (PAGEIA) to detect antibodies to SNV in deer mice ( 7 ). (cdc.gov)
The nine E-glycoprotein-reactive monoclonal antibodies also neutralized dengue 2 virus in a plaque reduction assay. (ajtmh.org)

To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (origene.com)

The primary antibody was detected with a 1:500 dilution of goat anti-mouse alexfluor-488 conjugated antibody. (cytoskeleton.com)

HAMA bridging interference produces artificially higher results because HAMA's bind to immobilized mouse antibodies in place of substrate, secondary labeled antibodies will then bind to HAMA and produce a positive signal falsely indicative of substrate presence. (wikipedia.org)
In this manner, HAMA provides a bridge between immobilized antibodies and labeled secondary antibodies. (wikipedia.org)
only the immobilized mouse antibodies and the HAMA remain in the immunoassay when the labeled secondary antibodies are administered. (wikipedia.org)
Since the substrate will no longer be able to bind to the immobilized mouse antibodies because of HAMA interference, labeled secondary antibodies will not give a signal for substrate presence. (wikipedia.org)
IRDye 680LT Secondary Antibodies are not intended for use in In-Cell Western ™ Assays or in vivo imaging applications. (licor.com)

Antibodies to Y. enterocolitica were raised for rapid Yersinia detection in the stool. (who.int)

Our new knob-into-hole bispecific antibodies illustrate how we can take antibody engineering concepts developed for human therapeutics and apply them back to research reagents, thereby bringing the technology back into the lab and opening up exciting new research possibilities for the scientific community. (nepic.co.uk)

Detects mouse FOLR1 in direct ELISAs. (rndsystems.com)
Detects mouse EphB2 in Western blots. (rndsystems.com)
The antibody detects endogenous Collagen II protein. (biocheminfo.org)
The MUC5AC Antibody detects for mucin 5AC (MUC5AC), a secretory-type mucin found in columnar mucous cells of surface gastric epithelium, in goblet cells of the fetal, and in precancerous colon, but is not found in normal colon cells. (neuromics.com)

Immunofluorescence images of mouse Swiss 3T3 cells stained with anti-actin antibody. (cytoskeleton.com)
Immunofluorescence staining using 1:500 dilution of anti-actin antibody in PBS is shown (green). (cytoskeleton.com)

Mouse Anti Human Monoclonal. (prospecbio.com)
The Committee was informed of progress in research into chimeric chimpanzee/human monoclonal antibodies. (who.int)

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. (origene.com)

Western blot analysis of PTEN expression in mouse PTEN transfected 293T whole cell lysate (lane 2) and non-transfected 293T lysate (lane 3). (licor.com)
Western blot analysis of BCMA: Lane 1: MW marker, Lane 2: Mouse BCMA Recombinant Protein. (enzolifesciences.com)
Western blot of purified actin and cell extracts probed with anti-actin antibody. (cytoskeleton.com)

This study investigated the monoclonal antibody aducanumab against amyloid plaques in the brain. (medscape.com)
They were then treated in the dose-finding study with one monthly infusion of 1, 3, 6, or 10 mg of the antibody aducanumab per kilogram of body weight. (medscape.com)
Aducanumab was compared, in a blinded study, to three clinical-stage antibodies using chemical kinetics. (lu.se)
The publication describes the use of chemical kinetic analysis to assess the mechanisms of action of four clinical stage anti-Aβ antibodies: aducanumab (Biogen), gantenerumab (Roche), bapineuzumab (Elan) and solanezumab (Eli Lilly). (lu.se)

Mice were intraperitoneally injected with STZ or sodium citrate buffer (CB) 7 days before subcutaneous inoculation with MC38 cells in the right flank on day 0 (Fig. 1 A). Then, the mice were treated with anti-PD-1 antibody or normal rat IgG on days 5, 8, and 11. (nature.com)

The IgG fraction was purified from mouse ascites by chromatographic procedures not involving affinity purification. (jacksonimmuno.com)

All antibodies are produced recombinantly for ensured batch-to-batch reproducibility, and offer high purity and low endotoxin levels ideal for in vivo applications. (nepic.co.uk)

To induce hyperglycemia, mice were intraperitoneally injected with STZ, which can damage pancreatic β cells resulting in the development of insulin dependent diabetes, a model of type 1 diabetes mellitus (T1DM). (nature.com)
Mice of C57BL/6 strain were singly injected intraperitoneally with antibodies (AB) to serotonin (5-HT). (unboundmedicine.com)
Nude female BALB/c mice, 8- to 12-wk old, were injected intraperitoneally or intravenously. (snmjournals.org)

It also reacts with the light chains of other mouse immunoglobulins. (jacksonimmuno.com)

however, it remains unknown whether the anti-tumor effects of anti-programmed cell death-1 antibody (PD-1-Ab) treatment are changed in hyperglycemic conditions. (nature.com)
We analyzed the effect of PD-1-Ab on tumor growth in streptozotocin-induced diabetic mice (STZ-mice) subcutaneously inoculated with MC38 (a colon carcinoma cell line). (nature.com)
Furthermore, we assessed the expression of chemokines by polymerase chain reaction (PCR) array in tumor-draining lymph nodes (dLNs) of these mice and MC38 cells cultured in different glucose concentrations. (nature.com)
This was accompanied by fewer tumor-infiltrating CD8 + T cells, and STZ-mice had fewer tumor-infiltrating CD11c + dendritic cells (DCs) than normoglycemic mice. (nature.com)
Immune checkpoint inhibitors (ICIs), including antibodies against programmed cell death-1 (PD-1) and PD-1 ligand-1 (PD-L1), induce their anti-tumor effects by improving the function of immune cells 11 . (nature.com)
To investigate the effect of hyperglycemia on anti-tumor function induced by anti-PD-1 antibody treatment, we treated STZ-induced diabetic mice with subcutaneous MC38 colon carcinoma tumors with anti-PD-1 antibody, and examined changes in tumor growth, the number of tumor-infiltrating immune cells, and the expression of chemokines and chemokine receptors. (nature.com)
The effect of anti-PD-1 antibody on MC38 tumor is attenuated in STZ-induced diabetic mice. (nature.com)
Positron emission tomography (PET)/computed tomography images clearly showed that Zr-Df-F(ab') possessed superior pharmacokinetics and imaging contrast over the radiolabeled full antibody, with much earlier and higher tumor uptake (5.5 times more at 2 h post injection) and much lower liver background (51% reduction at 2 h post injection). (physiciansweekly.com)

For comparison, another group of mice was infected with Zika virus and then given a placebo. (wustl.edu)

Mice treated in this way will give birth to pups that show some signs of brain and nervous damage by Zika, as well as other abnormalities similar, if not exactly identical, to those found in humans. (sciencemediacentre.org)

We developed a 1-hour field enzyme immunoassay (EIA) for detecting antibody to Sin Nombre virus in deer mice ( Peromyscus maniculatus ). (cdc.gov)

The CD40-CD40L interaction plays a critical role in both humoral and cellular immune responses and interfering antibodies have been suggested as an effective approach for the treatment of lymphomas and autoimmune diseases. (lu.se)
We believe that this analysis of several mouse anti-CD40 antibodies can be used to develop strategies for producing new human anti-CD40 antibodies that can more effectively induce or block B-cell proliferation. (lu.se)

The B3B4 antibody is useful for blocking IgE activity in vivo . (biolegend.com)
Of the neutralizing monoclonal antibodies, five passively protected mice in vivo against lethal intracerebral dengue 2 challenge. (ajtmh.org)

For several decades, and until recently, mice were used extensively in the production of monoclonal antibodies (MAbs). (wikipedia.org)
The rationale of this study was to determine the myelotoxicity in nude mice of the α-emitter 211 At conjugated to monoclonal antibodies (mAbs) and to compare the effect with an electron emitter, 99m Tc, and external irradiation from a 60 Co source, for estimation of the relative biological effectiveness (RBE). (snmjournals.org)
Coupled to monoclonal antibodies (mAbs), or fragments of antibodies, α-particle emitters provide a promising tool in treating different kinds of disseminated cancers. (snmjournals.org)

In North America, the principal cause of HPS is Sin Nombre virus (SNV) because of the geographically widespread nature of its rodent host, the deer mouse ( Peromyscus maniculatus ), the most common mammal in North America. (cdc.gov)
the technique could be exploited for further investigations of the virus-reservoir host interactions and characteristics and to determine whether experimental infections of deer mice with SNV accurately parallel natural infections ( 3 , 4 ). (cdc.gov)

Anatomy21

Organisms20

Diseases6

Chemicals and Drugs114

Analytical, Diagnostic and Therapeutic Techniques and Equipment56

Phenomena and Processes44

Disciplines and Occupations2

Information Science3

Health Care5

Reactivity7

Specificity3

ELISA3

Immunogen4

Isotype3

Proteins5

Antigen4

Polyclonal antibody to mouse2

Species3

Murine7

FITC2

Cross react1

Catalog3

Reacts with mouse1

Recombinant Antibody1

Immunoglobulin2

Conjugates4

IgG12

Assay2

Conjugation1

Goat1

Secondary5

Detection1

Reagents1

Detects4

Immunofluorescence2

Human Monoclonal2

Concentration1

Western Blot3

Aducanumab4

Buffer1

Ascites1

Purity1

Intraperitoneally3

Immunoglobulins1

Tumor8

Infected with Zika virus1

Found in humans1

Immunoassay1

CD402

Vivo2

MAbs3

Host2