• It also associates with the general transcriptional machinery and may influence transcription initiation and promoter selection by interacting with RNA polymerase II and the TFIID complex. (wikipedia.org)
  • EWS binds RNA, calmodulin, and the RNA polymerase II 33 kD polypeptide (RPB3), but not RPB5 or RPB7. (medindex.am)
  • Within SEC two elongation factors, positive transcription elongation factor b (P-TEFb) and elongation factor for RNA polymerase II 2 (ELL2) are key components. (biomedcentral.com)
  • Here we show, however, that RNA polymerase II (Pol II) inside human nucleoli operates near genes encoding rRNAs to drive their expression. (nature.com)
  • Mechanistically, SEs recruited bromodomain containing 4 (BRD4) at the site of ZBTB16 , which then bound to RNA polymerase II-associated protein 2 (RPAP2) that transported RNA polymerase II (POL II) into the nucleus. (nature.com)
  • Short calmodulin-binding motif containing conserved Ile and Gln residues. (embl.de)
  • Calmodulin-binding motif. (embl.de)
  • The prooncoprotein EWS binds calmodulin and is phosphorylated by protein kinase C through an IQ domain. (embl.de)
  • Here we report that EWS, a nuclear RNA-binding prooncoprotein, contains an IQ domain, is phosphorylated by protein kinase C, and interacts with calmodulin. (embl.de)
  • Sequence motifs for calmodulin recognition. (embl.de)
  • Calmodulin (CaM) is recognized as a major calcium sensor and orchestrator of regulatory events through its interaction with a diverse group of cellular proteins. (embl.de)
  • Additionally, FUS/TLS has been found to bind a relatively long region in the 3′ untranslated region (UTR) of the actin-stabilising protein Nd1-L mRNA, suggesting that rather than recognising specific short sequences, FUS/TLS interacts with multiple RNA-binding motifs or recognises secondary conformations. (wikipedia.org)
  • EWS interacts with FAK2 and relocates from cytoplasm to ribosomes upon FAK2 activation. (medindex.am)
  • RNA-binding protein FUS/TLS (FUsed in Sarcoma/Translocated in LipoSarcoma), also known as heterogeneous nuclear ribonucleoprotein P2 is a protein that in humans is encoded by the FUS gene. (wikipedia.org)
  • Ubiquitous nuclear and cytoplasmic RNA Binding Protein EWS belongs to the TET family of RNP proteins, contains an RNA recognition motif and a RANBP2-type zinc finger, and may function as a repressor. (medindex.am)
  • Our findings revise theories of labour division between the major RNA polymerases, and identify nucleolar Pol II as a major factor in protein synthesis and nuclear organization, with potential implications for health and disease. (nature.com)
  • For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
  • Ribonucleoproteína nuclear heterogénea que se encuentra en el NÚCLEO CELULAR y en el CITOPLASMA. (bvsalud.org)
  • Se ha implicado a la ribonucleoproteína nuclear heterogénea K en la regulación de la expresión genética en casi todos los niveles: TRANSCRIPCIÓN GENÉTICA, procesamiento del ARNm (PROCESAMIENTO DEL ARN POSTRANSCRIPCIONAL), transporte del ARNm, estabilidad del ARNm y traducción (TRADUCCIÓN GENÉTICA). (bvsalud.org)
  • I was able to further validate these binding sites by designing cell permeable peptides designed to disrupt protein binding in vitro. (gla.ac.uk)
  • Intracellular Signaling Peptides and Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
  • Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. (jefferson.edu)
  • Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. (jefferson.edu)
  • Below are the most recent publications written about "Intracellular Signaling Peptides and Proteins" by people in Profiles. (jefferson.edu)
  • Proto-Oncogene Proteins c-mdm2" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • This graph shows the total number of publications written about "Proto-Oncogene Proteins c-mdm2" by people in this website by year, and whether "Proto-Oncogene Proteins c-mdm2" was a major or minor topic of these publications. (umassmed.edu)
  • Below are the most recent publications written about "Proto-Oncogene Proteins c-mdm2" by people in Profiles. (umassmed.edu)
  • Proto-oncogene proteins that negatively regulate RECEPTOR PROTEIN-TYROSINE KINASE signaling. (nih.gov)
  • FUS/TLS, EWS and TAF15 have a similar structure, characterised by an N-terminal QGSY-rich region, a highly conserved RNA recognition motif (RRM), multiple RGG repeats, which are extensively demethylated at arginine residues and a C-terminal zinc finger motif. (wikipedia.org)
  • Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. (embl.de)
  • The 3',5'-cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) are known to play a role in disease progression as they mediate downstream signalling pathways that can promote cell growth and disease progression. (gla.ac.uk)
  • Canonical Notch signaling activates the transcription of BMI1 proto‑oncogene polycomb ring finger, cyclin D1, CD44, cyclin dependent kinase inhibitor 1A, hes family bHLH transcription factor 1, hes related family bHLH transcription factor with YRPW motif 1, MYC, NOTCH3, RE1 silencing transcription factor and transcription factor 7 in a cellular context‑dependent manner, while non‑canonical Notch signaling activates NF‑κB and Rac family small GTPase 1. (spandidos-publications.com)
  • 32 and 36 by IjBa kinase, resulting in the conju-gation of ubiquitin that targets the proteins for pro-teasome-mediated degradation. (lsd1receptor.com)
  • These data suggest that PKC may regulate interactions of EWS and other RNA-binding proteins with their RNA targets and that IQ domains may provide a regulatory link between Ca2+ signal transduction pathways and RNA processing. (embl.de)
  • Interestingly, PKC phosphorylation of EWS inhibits its binding to RNA homopolymers, and conversely, RNA binding to EWS interferes with PKC phosphorylation. (embl.de)
  • PKC phosphorylation of these proteins also inhibits their binding to RNA in vitro. (embl.de)
  • MDM2 binds to and inhibits TP53 activity. (medscape.com)
  • Three recognition motifs for CaM interaction are discussed in the context of experimental investigations of a variety of CaM target proteins. (embl.de)
  • Although considerable sequence diversity is observed among the different binding regions, these three classes of recognition motifs exist for many of the known CaM binding proteins. (embl.de)
  • RPAP2 transports RNA Pol II from the cytoplasm to the nucleus and guides Pol II to target ZBTB16 via recognition of the navigator BRD4 on SEs. (nature.com)
  • DHX9 is an RNA/DNA helicase that is involved in multiple cellular processes, including transcription and maintaining genome stability. (gla.ac.uk)
  • Furthermore, PDE4D7 was found to bind within DHX9's helicase core domain, suggesting that it may play a role in regulating its activity. (gla.ac.uk)
  • A later proposal was that the GGUG motif is recognised by the zinc finger domain and not the RRM (80). (wikipedia.org)
  • Consistently, in vitro studies have shown that FUS/TLS binds RNA, single-stranded DNA and (with lower affinity) double-stranded DNA. (wikipedia.org)
  • however, using in vitro selection (SELEX), a common GGUG motif has been identified in approximately half of the RNA sequences bound by FUS/TLS. (wikipedia.org)
  • FUS/TLS has also been proposed to bind human telomeric RNA (UUAGGG)4 and single-stranded human telomeric DNA in vitro. (wikipedia.org)
  • This was independent of the increase in reactive oxygen species, p38MAPK phos-phorylation and expression of NF-jB target proteins. (lsd1receptor.com)
  • Ras GTPase-activating-like protein IQGAP1. (embl.de)
  • IQGAP1 contains 4 IQ motifs. (embl.de)
  • The hnRNP protein has a strong affinity for polypyrimidine-rich RNA and for single-stranded polypyrimidine-rich DNA. (bvsalud.org)
  • FUS/TLS was independently identified as the hnRNP P2 protein, a subunit of a complex involved in the maturation of pre-mRNA. (wikipedia.org)
  • The p50 sub-unit lacks a transactivation domain and is believed to act as a regulatory subunit, modulating the DNA-binding affinity of p65 [14,15]. (lsd1receptor.com)
  • Poly mRNA was isolated from the total RNA sam ples with oligo magnetic beads. (sykinhibitors.com)
  • The purified mRNA was fragmented through the RNA fragmenta tion kit and utilized as template for very first strand cDNA synthesis making use of random hexamer primers and reverse transcriptase. (sykinhibitors.com)
  • Two isoforms of the EWS protein are produced by differential splicing. (medindex.am)
  • Multiple hnRNP K protein isoforms exist due to alternative splicing and display different nucleic-acid-binding properties. (bvsalud.org)
  • Because CaM can bind with high affinity to a relatively small alpha-helical region of many proteins, success in clearly defining the essential elements of CaM binding motifs seems feasible and should provide a means of identifying CaM binding proteins. (embl.de)
  • For this step to be efficiently executed, the viral Trans-Activator of Transcription, Tat, acts as a master regulator of transcription elongation by tethering SEC to the HIV trans-activator response (TAR) stem-loop RNA on the viral short transcripts, and synergistically enhancing RNAPII pause-release and elongation. (biomedcentral.com)
  • It is also striking to note that, at least in terms of viral RNA, Omicron replicated better in pediatric NECs compared to both Delta and the ancestral virus. (plos.org)
  • The shield prevents Pol I from producing sense intergenic noncoding RNAs (sincRNAs) that can disrupt nucleolar organization and rRNA expression. (nature.com)
  • Interestingly, DHX9 maps to the PC susceptibility locus, making it an interesting protein to study in the context of PC disease progression and metastasis. (gla.ac.uk)
  • Existing models suggest that RNA polymerases I and III (Pol I and Pol III) are the only enzymes that directly mediate the expression of the ribosomal RNA (rRNA) components of ribosomes. (nature.com)
  • FUS/TLS is a member of the FET protein family that also includes the EWS protein, the TATA-binding protein TBP-associated factor TAFII68/TAF15, and the Drosophila cabeza/SARF protein. (wikipedia.org)
  • Many investigations have focused on defining the region of interaction between CaM and its cellular targets and the action of CaM on target protein function. (embl.de)
  • By using peptide array technology, I was able to map where this interaction took place and define docking sites on both protein partners. (gla.ac.uk)
  • FUS gene rearrangement has been implicated in the pathogenesis of myxoid liposarcoma, low-grade fibromyxoid sarcoma, Ewing sarcoma, and a wide range of other malignant and benign tumors (see FET protein family). (wikipedia.org)
  • During osteogenesis, histones on osteogenic identity genes are acetylated, and OB-gain SEs appear, enabling the binding of BRD4 to the osteogenic identity gene ZBTB16. (nature.com)
  • The scripts used for RNA-seq data analysis including differential gene expression and gene set enrichment analysis can be found in https://github.com/akaraw/Yanshan_Zhu_et_al . (plos.org)
  • [ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
  • Benefits Diverse categories of pathways are activated in uFB and PD We carried out GSEA of gene transcription and protein expression information obtained in human HCC cell lines cultured in uFB and PD. (sykinhibitors.com)
  • Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors. (jefferson.edu)
  • In these instances, the promoter and N-terminal part of FUS/TLS is translocated to the C-terminal domain of various DNA-binding transcription factors (e.g. (wikipedia.org)
  • Treating 293T cells with a histone deacetylase inhibitor increased acetylated histone H4 binding to the NR0B1 promoter and activated NR0B1 expression. (oncotarget.com)
  • Furthermore, the level of acetyl-histone H4 binding to the NR0B1 promoter increased, whereas the occupancy of H3K27me3 was lower in cancerous tissues than in non-cancerous tissues. (oncotarget.com)
  • In conclusion, this study shows that CpG methylation within the NR0B1 promoter is not involved in the in vivo regulation of NR0B1 expression, whereas the hyperacetylation of histone H4 and the unmethylation of histones H3K9 and H3K27, and their binding to the NR0B1 promoter results in decondensed euchromatin for NR0B1 activation. (oncotarget.com)
  • DHX9 was found to bind within the newly identified FLY multi docking site within the upstream conserved region-1 (UCR1) of PDE4D7. (gla.ac.uk)
  • La proteína hnRNP tiene una fuerte afinidad por el ARN rico en polipirimidina y por el ADN de hebra única rico en polipirimidina. (bvsalud.org)
  • Existen múltiples isoformas de la proteína K hnRNP debido a ayuste alternativo y muestran características diferentes en la unión al ácido nucleico. (bvsalud.org)
  • Beyond nucleic acid binding, FUS/TLS was also found to associate with both general and more specialized protein factors to influence the initiation of transcription. (wikipedia.org)
  • A modified version of the IQ motif as a consensus for Ca2+-independent binding and two related motifs for Ca2+-dependent binding, termed 18-14 and 1-5-10 based on the position of conserved hydrophobic residues, are proposed. (embl.de)
  • We reveal a nucleolar Pol-II-dependent mechanism that drives ribosome biogenesis, identify disease-associated disruption of nucleoli by noncoding RNAs, and establish locus-targeted R-loop modulation. (nature.com)
  • The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)
  • This locus, however, also encodes a protein from an alternative reading frame, designated p19ARF. (medscape.com)
  • The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)
  • His research utilizes the tools of synthetic chemistry, protein biochemistry, and cancer biology to discover and validate new strategies for the inhibition of anti-cancer targets. (stanford.edu)
  • RNA-seq data is deposited at European Nucleotide Archive under the project- PRJEB43102. (plos.org)
  • Lastly, the distinct effects of matched protein expression data from the two groups led us to investigate the function of ubiquitination during the onset of oncogenic properties. (sykinhibitors.com)
  • However, close examination showed the results of protein data inclusion were rather opposite within the uFB and PD groups In uFB, the total quantity of pathways was decreased, whilst new pathways such as RIG I like receptor signaling were activated. (sykinhibitors.com)
  • CHOP) conferring a strong transcriptional activation domain onto the fusion proteins. (wikipedia.org)
  • Taxonomic distribution of proteins containing IQ domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with IQ domain is also avaliable . (embl.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing IQ domain in the selected taxonomic class. (embl.de)
  • EWS contains a transcription activation domain, but the RNA-binding domain can repress trans-activation by the activation domain. (medindex.am)
  • Without SEs located on osteogenic genes, BRD4 is not able to bind to osteogenic identity genes due to its closed structure before osteogenesis. (nature.com)
  • Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
  • Here, it binds to specific jB elements to drive transcription of target genes [14-20]. (lsd1receptor.com)
  • There are 165951 IQ domains in 60575 proteins in SMART's nrdb database. (embl.de)
  • The RNA-binding activity is localized to a C-terminal RGG box. (medindex.am)
  • The exact mechanism of fen-retinide-induced cell death is not fully understood, although it is reported to induce apoptosis indepen-dently of retinoic acid receptor expression [6-9], lacking the carboxyl group required for binding to these receptors [10]. (lsd1receptor.com)
  • FUS/TLS was initially identified as a fusion protein (FUS-CHOP) produced as a result of chromosomal translocations in human cancers, especially liposarcomas. (wikipedia.org)
  • NR0B1 protein was detected in cancerous tissues of more than 50% of human lung adenocarcinoma (ADCA) cases and tended to be expressed in low-differentiated cancerous tissues obtained from males. (oncotarget.com)
  • Proteomics-Based Discovery of First-in-Class Chemical Probes for Programmed Cell Death Protein 2 (PDCD2). (stanford.edu)
  • B cell scaffold protein with ankyri. (gsea-msigdb.org)
  • The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
  • By tracking the changes of more than 1,000 postsynaptic density (PSD) proteins from midgestation to young adulthood, we find that PSD maturation in humans separates into three major phases that are dominated by distinct pathways. (bvsalud.org)
  • After the binding of the RPAP2-Pol II complex to BRD4 on SEs, RPAP2 dephosphorylates Ser5 at the Pol II CTD to terminate the transcriptional pause, and BRD4 phosphorylates Ser2 at the Pol II CTD to initiate transcriptional elongation, which synergistically drives efficient transcription of ZBTB16, ensuring proper osteogenesis. (nature.com)
  • adaptor related protein complex 1 s. (gsea-msigdb.org)
  • The N-terminal end of FUS appears to be involved in transcriptional activation, while the C-terminal end is involved in protein and RNA binding. (wikipedia.org)